CN104111277A - Electroanalytical chemical evaluation method for catalysis performance of biological nanometer metal - Google Patents
Electroanalytical chemical evaluation method for catalysis performance of biological nanometer metal Download PDFInfo
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- CN104111277A CN104111277A CN201310136617.1A CN201310136617A CN104111277A CN 104111277 A CN104111277 A CN 104111277A CN 201310136617 A CN201310136617 A CN 201310136617A CN 104111277 A CN104111277 A CN 104111277A
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Abstract
The invention belongs to the field of materials and biological electrochemistry, and relates to an electroanalytical chemical evaluation method for the catalysis performance of a biological nanometer metal. A synthetic method for a nanometer metal (Au and Pd) comprises: under an anaerobic condition, taking a lactate as an electron donor, and enabling shewanella sp. and a sulfate-reducing bacteria to be co-cultured with HAuCl4 or (NH4)2PdCl4 (the concentration of Au and Pd are respectively 0.05 mM and 0.07 mM) for a period. The electroanalytical chemical evaluation method comprises: centrifuging the above bacterial liquid to remove the supernatant, washing with sterile water, taking 5 mu L of the obtained thallus precipitate and dropwise adding on the surface of a glassy carbon electrode, and naturally airing, so as to obtain a working electrode; and employing a three-electrode system taking an Ag/AgCl electrode as a reference electrode and platinum wire electrode as a counter electrode and performing cyclic voltammetry scanning and chronoamperometric determination, taking a phosphate buffer with the concentration of 50 mM (pH 7.00) as an electrolyte, and controlling the cyclic voltammetry scanning rate to be 10 mV/s. The obtained microbial nanometer gold and palladium have good catalytic performance, and are capable of well catalyzing oxidation of a lactate and reduction of oxygen.
Description
Technical field
The present invention relates to the bacterium that two strains have electrochemical activity, comprise a strain Shewanella and a strain of sulfate reduction bacteria, two bacterial strains can be through respiration synthesis of nano metal (Au, Pd), and after synthesis of nano metal, still has electrochemical activity.In addition, the invention still further relates to the catalytic performance of the biological nano metal that utilizes electroanalysis chemical process (cyclic voltammetry, chronoamperometry) evaluation synthesized, belong to materialogy and bioelectrochemistry field.
Background technology
Utilizing microorganism synthesis of nano metal, is a kind of quick, gentle, eco-friendly method.Shewanella and sulphate reducing bacteria can, by the respiration of self, be reduced to nano particle by soluble metal ion in environment.Because metal in this process is in intracellular immobilization, can alleviate to a certain extent the heavy metal pollution in environment.The synthetic nano metal of Shewanella and sulphate reducing bacteria is embedded on cell membrane, and its particle diameter is little, and even particle distribution has higher kinetic activity.The nano metal that this mode forms not only has good biocompatibility, and because gold, palladium itself have good conduction and catalytic capability, its electrochemical signals is also very easily caught in.
Electroanalytical Chemistry is the electrochemical properties in solution and change the method analyzed according to material, using electricity lead, relation between the electrochemical parameter such as current potential, electric current and electric weight and measured matter content is as the basis of measuring.The present invention, by the change of redox peak current in analysis cycle volt-ampere curve and peak position, observes the current-responsive in chronoa mperometric plot, evaluates the catalytic capability of biological nano metal pair material.The method analysis speed is fast; Selectivity is good highly sensitive; Consumption is few, is applicable to carry out micromanipulation; Instrument is simple, and economy is also easy to automatic control.
Summary of the invention
The object of this invention is to provide a kind of method of utilizing Electroanalytical Chemistry means evaluation biological nano metal catalytic performance.
In the present invention, the concrete steps of synthesis of nano metal are as follows.
1: centrifugal cultured bacterium liquid (6000 r/min, 5 min) are removed to supernatant, with after sterile distilled water washing precipitation 3 times, thalline is resuspended in the sterile distilled water containing sodium lactate (50 mM).
2: the resuspended liquid of 1 thalline of obtaining is placed in to serum bottle, keeps its anaerobic condition.
3: with syringe by a small amount of high concentration HAuCl
4or (NH
4)
2pdCl
4aqueous solution is injected serum bottle, makes Au, Pd ultimate density be respectively 0.05,0.07 mM.
After 4:30 DEG C of cultivation 1h, form nm of gold, palladium.
In the present invention, the mensuration of electrochemical activity is to adopt cyclic voltammetry.Concrete operation step is as follows.
1: the bacterium liquid centrifugal (6000 r/min, 5 min) of cultivating is as stated above removed to supernatant, with aseptic distilled water washing precipitation 3 times, obtain clean bacterial sediment.
2: 1 bacterial sediment of obtaining is got to 5 ul and be added drop-wise on clean glass-carbon electrode, naturally dry, thalline is fixed on electrode.
3: adopt three-electrode system to carry out electrochemistry experiment, 2 electrodes that prepare are working electrode, and Ag/AgCl electrode is contrast electrode, and platinum electrode is to electrode.
4: make electrolytic solution with the phosphate buffer (pH 7.00) of 50 mM, sweep speed is 10 mV/s.
The Electroanalytical Chemistry assessment method of biological nano metal catalytic performance provided by the present invention, simple, convenient quick, synthetic microorganism nano metal has good catalysis lactate oxidation and the ability of oxygen reduction.
Brief description of the drawings
Fig. 1: under anaerobic condition, the cyclic voltammogram after Shewanella synthesis of nano gold.
Fig. 2: under anaerobic condition, the chronoamperogram after Shewanella synthesis of nano gold.
Fig. 3: the synthetic Technique of Nano Pd of sulphate reducing bacteria is modified at the cyclic voltammogram on glass-carbon electrode.
Embodiment
Embodiment 1: a in Fig. 1: the cyclic voltammogram after Shewanella synthesis of nano gold; B: after Shewanella synthesis of nano gold, adding sodium lactate is the cyclic voltammogram of substrate.Fig. 1 demonstration adds sodium lactate to do after substrate, and the oxidation current of Shewanella is significantly increased, and this shows the Shewanella tool electrochemical activity after synthesis of nano gold, and oxidation has certain catalytic action to lactate.
Embodiment 2: because the cyclic voltammetry curve of Fig. 1 is mainly manifested in the oxidation peak of 0.2 V the current-responsive of sodium lactate after showing Shewanella synthesis of nano gold, therefore by voltage stabilization at 0.2V, carry out the mensuration of timing electric current.Fig. 2 shows, when after the Shewanella current stabilization after synthesis of nano gold, adding sodium lactate is substrate, electric current 10 nA that raise rapidly.This result shows, the Shewanella after synthesis of nano gold has good current-responsive to lactate, the Lactated oxidation of catalysis well.
Embodiment 3: Fig. 3 has shown the Technique of Nano Pd that sulphate reducing bacteria is synthetic, at a: anaerobic, b: the cyclic voltammogram under aerobic conditions.Pass into after oxygen, the reduction current of cyclic voltammetric enlarges markedly and occurs a reduction peak, and the emerging reduction peak of empirical tests comes from oxygen.The synthetic Technique of Nano Pd of this explanation sulphate reducing bacteria is a kind of good oxygen reduction catalyzer.
Claims (2)
1. a method of utilizing Shewanella and sulphate reducing bacteria synthesis of nano gold, palladium, comprising: by the centrifugal cultured bacterium liquid supernatant that goes, clean with sterile distilled water; Under anaerobic, clean bacterial strain, taking lactate as electron donor, is cultivated a period of time altogether with gold, palladium salt solusion, forms nm of gold, palladium.
2. the catalytic performance that utilizes electroanalysis chemical process evaluation biological nano metal, comprises the preparation of working electrode and the mensuration of electrochemical properties; Working electrode preparation: centrifugal thalline, sterile distilled water are added drop-wise to electrode surface after cleaning, are prepared into working electrode after naturally drying; Electrochemical properties is measured: utilize three-electrode system, carry out cyclic voltammetry scan and timing amperometric determination; Add the variation of substrate front and back redox electric current and the current-responsive of electric current-time curve by contrast cyclic voltammogram, evaluate the catalytic performance of microorganism nano metal to added substrate.
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| CN201310136617.1A CN104111277A (en) | 2013-04-19 | 2013-04-19 | Electroanalytical chemical evaluation method for catalysis performance of biological nanometer metal |
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| CN201310136617.1A CN104111277A (en) | 2013-04-19 | 2013-04-19 | Electroanalytical chemical evaluation method for catalysis performance of biological nanometer metal |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104588677A (en) * | 2014-12-04 | 2015-05-06 | 华南理工大学 | Method for synthesizing shewanella halitios into god nanoparticles and application of gold nanoparticles |
| CN105780067A (en) * | 2016-02-01 | 2016-07-20 | 中国科学院生态环境研究中心 | Method for in-situ synthesis of three-dimensional nanometer palladium catalyst layer through electrode activity biological membrane and application |
| CN113186228A (en) * | 2021-05-12 | 2021-07-30 | 天津城建大学 | Microbial supported palladium-gold bimetallic nano-catalyst and preparation method and application thereof |
| CN114324513A (en) * | 2021-11-29 | 2022-04-12 | 郑州大学 | Poly-L-phenylalanine/nanogold/Shewanella MR-1/multi-walled carbon nanotube modified electrode, preparation method and application |
-
2013
- 2013-04-19 CN CN201310136617.1A patent/CN104111277A/en active Pending
Non-Patent Citations (5)
| Title |
|---|
| K. DEPLANCHE,ET AL.: "Biorecovery of Gold by Escherichia coli and Desulfovibrio desulfuricans", 《BIOTECHNOLOGY AND BIOENGINEERING》 * |
| RANRAN WU,ET AL.: "Effects of Bio-Au Nanoparticles on Electrochemical Activity of Shewanella oneidensis Wild Type and DomcA/mtrC Mutant", 《SCIENTIFIC REPORTS》 * |
| SIMON DE CORTE,ET AL.: "Gold nanoparticle formation using Shewanella oneidensis: a fast biosorption and slow reduction process", 《J CHEM TECHNOL BIOTECHNOL》 * |
| XUEE WU, ET AL.: "A Role for Microbial Palladium Nanoparticles in Extracellular Electron Transfer", 《ANGEW. CHEM. INT. ED.》 * |
| YASUHIRO KONISHI,ET AL.: "Intracellular recovery of gold by microbial reduction of AuCl4- ions using the anaerobic bacterium Shewanella algae", 《HYDROMETALLURGY》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104588677A (en) * | 2014-12-04 | 2015-05-06 | 华南理工大学 | Method for synthesizing shewanella halitios into god nanoparticles and application of gold nanoparticles |
| CN105780067A (en) * | 2016-02-01 | 2016-07-20 | 中国科学院生态环境研究中心 | Method for in-situ synthesis of three-dimensional nanometer palladium catalyst layer through electrode activity biological membrane and application |
| CN113186228A (en) * | 2021-05-12 | 2021-07-30 | 天津城建大学 | Microbial supported palladium-gold bimetallic nano-catalyst and preparation method and application thereof |
| CN113186228B (en) * | 2021-05-12 | 2023-01-17 | 天津城建大学 | Microbial supported palladium-gold bimetallic nano-catalyst and preparation method and application thereof |
| CN114324513A (en) * | 2021-11-29 | 2022-04-12 | 郑州大学 | Poly-L-phenylalanine/nanogold/Shewanella MR-1/multi-walled carbon nanotube modified electrode, preparation method and application |
| CN114324513B (en) * | 2021-11-29 | 2023-11-03 | 郑州大学 | Poly L-phenylalanine/nano gold/Shewanella MR-1/multiwall carbon nanotube modified electrode, preparation method and application |
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Application publication date: 20141022 |