CN104107457A - 3D (three-dimensional) bio-printing hydrogel material and application thereof - Google Patents
3D (three-dimensional) bio-printing hydrogel material and application thereof Download PDFInfo
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- CN104107457A CN104107457A CN201410363077.5A CN201410363077A CN104107457A CN 104107457 A CN104107457 A CN 104107457A CN 201410363077 A CN201410363077 A CN 201410363077A CN 104107457 A CN104107457 A CN 104107457A
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Abstract
The invention discloses a 3D (three-dimensional) bio-printing hydrogel material and an application of the 3D bio-printing hydrogel material.The hydrogel material contains cell growth factors,nutrition components,water,a thermo-sensitive polymer and biological macro-molecules.The 3D bio-printing hydrogel material disclosed by the invention has similar mechanical properties with soft tissues of a human body, can be used for wrapping cells,and has good cell adhesiveness;when the 3D bio-printing hydrogel material is applied in the human body,the 3D bio-printing hydrogel material is small in immunological rejection and anti-allergic,is beneficial for health recovery of the human body,is good in biodegradability,safe and non-toxic,does not need secondary surgical removal,and can be used for reducing the secondary injury of a patient;the 3D bio-printing hydrogel material is matched with corresponding technological equipment,can achieve individualized designs on size and structure aspects,and can be used for providing'one-to-one'matching products for use objects.
Description
[technical field]
The present invention relates to a kind of 3D biometric print material and application thereof, relate in particular to a kind of 3D biometric print hydrogel material and application thereof based on biopolymer, belong to technical field of biological materials.
[background technology]
Tradition numerical control manufacture is generally on raw material basis, uses the ways such as cutting, grinding, burn into melting, removes redundance, obtains parts, then becomes final products with Combination of Methods such as assembled, welding.And 3D printing is that a kind of mathematical model file is for basic, use powdery metal or the plastics etc. can jointing material, carry out the technology of constructed object by the mode of successively stacking accumulation, therefore also referred to as " increasing material manufacture " (additive manufacturing).3D prints without proembryo and mould, just can directly generate the object of any shape according to computer graphics data, simplifies the fabrication schedule of product, shortens the lead time of product, raises the efficiency and reduces costs.
3D printing technique claims again to increase material manufacturing technology, is actually a kind of emerging technology in rapid shaping field, and it is a kind of taking mathematical model file as basis, carrys out the technology of constructed object by the mode of successively printing.3D printing technique has had the developing history of more than 30 year till now from the proposition of concept.Along with reaching its maturity of technology, equipment, 3D prints and starts to come into the popular visual field and become rapidly manufacturing new lover.And for medical profession, 3D printing technique is indicating that a new revolution of medical science maybe will arrive.From emulation medical treatment model, biomedical devices, to transplanted tissue or the trachea of more personalized, have more the bioabsorbable polymer material of potentiality, all will gather in 3D printing those under one's command.
Biometric print is the application that 3D is printed on medical field, and in order to manufacture living tissue and the organ of living, its application in organizational project becomes one of the most popular research topic already.In the end of the year 2009, Australian Invetech company and Organovo company of the U.S. announce to have developed hand in hand global first commercialization 3D biometric print machine, and printer has two printheads, and the most nearly 80,000 human body cells of a placement are called as " biological China ink "; Another printable " biochargeable paper ".The main component of so-called biochargeable paper is hydrogel, can be used as the support of Growth of Cells.
Hydrogel water content based on biomacromolecule is high, mechanical property is similar to soft tissue, have good biocompatibility, carry the high efficiency of nutrient and excretion metabolism thing and the great ability of parcel cell, these features make it be widely used in building tissue engineering bracket, drug controlled release system etc.
Biometric print can be realized personalized customization, makes and " part " of tissue matched, repairs and mouth cavity orthodontic etc. for complicated bone surgery, skull reparation, microtia.Scientist's maximum great ambition is just to print the human organ such as the heart, liver to solve the demand of global increasing wait Organ Transplantation Patients.The high level of architectural complexity of most of organs, usually forms the concurrent various function of waving by tens kinds of different cells, taking liver as example, and nearly 500 kinds of its biological functions of bringing into play.Say technically, by successively print along Z axis cell 3D printer can " build " and go out the tissue or the organ that " are built up " by cell, but from angle biology, if think, these tissues or organ are applicable to human implantation, need consideration how by extremely suitable position of dissimilar cell " printing ", so that it develops into gradually wanted tissue or organ as embryo; How also to consider, for these cells provide nutrient substance and oxygen, how to build a blood circulation.
Nowadays 3D prints and the hydrogel material that is applied to organizational project has all developed into certain stage, how both has been fused together to biometric print, has just become a new problem.Biometric print technology is in the ascendant, and major applications material also rests on phase of basic research, should consider physical chemistry and the biology performance of material, also needs to solve the problem of " printing " molding, also has a segment distance from practical application.
[summary of the invention]
The object of the invention is for the shortage of material that is applied at present biometric print, provide a kind of based on biomacromolecule, human body is little by immunologic rejection, and biological degradability is good, has the 3D biometric print hydrogel material of suitable mechanical property.
Another object of the present invention is to provide the application of above-mentioned 3D biometric print hydrogel material in 3D biometric print.
To achieve these goals, the present invention is by the following technical solutions:
A kind of 3D biometric print hydrogel material, is characterized in that containing cell growth factor and nutrition composition.
3D biometric print hydrogel material of the present invention, contains water, temperature sensing polymer and biomacromolecule.
The mass percent of the above-mentioned each component of the present invention is:
Temperature sensing polymer in the present invention is selected from poly-N-isopropyl propyl amides class, PEO-PPO-PEO triblock copolymer and has the modified natural polymer of warm degeneration, preferably wherein a kind of.
Biomacromolecule in the present invention is selected from one or more in cellulose, hyaluronic acid, chitosan, Sargassum acids natural polymer and modifier thereof.
In material of the present invention, also contain Photoepolymerizationinitiater initiater, cross-linking agent and other regulators, wherein Photoepolymerizationinitiater initiater and cross-linking agent can select its kind one or both, the two is all for regulating and controlling the degree of cross linking of network structure of hydrogel.
3D biometric print hydrogel material of the present invention is preferably made up of the component of following quality percentage composition:
The percentage ratio sum of each component is 100% above.
Temperature sensing polymer in the present invention is the one in poly-N-isopropyl propyl amides (p (NIPAAm)) class, PEO-PPO-PEO triblock copolymer (poloxamer) and the modified natural polymer with warm degeneration.
Biomacromolecule in the present invention is one or more in cellulose, hyaluronic acid, chitosan, Sargassum acids natural polymer and modifier thereof.
Time prepared by 3D biometric print hydrogel material of the present invention by each component mix homogeneously under room temperature.
The application of 3D biometric print hydrogel material of the present invention in 3D biometric print, the present invention is easy to molding while being applied to 3D biometric print.
The present invention, with respect to prior art, has following advantage:
When 3D stamping ink gel rubber material of the present invention is interior for human body, immunologic rejection is little, and antiallergic, without a large amount of anti-repulsions of long-term taking and Claritin, is conducive to health and recovers.Biological degradability is good, and safety non-toxic is operated on taking-up without secondary, has reduced patient's secondary damage.
3D stamping ink gel rubber material of the present invention has the mechanical property similar with human body soft tissue, adjustable porosity, and can wrap up cell, there is good cell adhesion, for guiding its growth, propagation to provide support skeleton and suitable physiological condition.
3D stamping ink gel rubber material of the present invention, coordinates corresponding technical equipment, can realize the personalized designs of the aspects such as its size, structure, for using object that the product of " one to one " coupling is provided.
[detailed description of the invention]
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 80%, temperature sensing polymer 10%, biomacromolecule 6.5%, cell growth factor and nutrition composition 1%, 1 part of Photoepolymerizationinitiater initiater, 1 part of cross-linking agent, 0.5 part of other regulator.
Embodiment 2:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 85%, temperature sensing polymer 5%, biomacromolecule 8%, Photoepolymerizationinitiater initiater 1%, cell growth factor and nutrition composition 1%.
Embodiment 3:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 86.5%, temperature sensing polymer 5%, biomacromolecule 5%, cell growth factor and nutrition composition 1%, Photoepolymerizationinitiater initiater 1%, cross-linking agent 1%, other regulators 0.5%.
Embodiment 4:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 50%, temperature sensing polymer 15%, biomacromolecule 25%, cell growth factor and nutrition composition 5%, Photoepolymerizationinitiater initiater 2%, cross-linking agent 2%, other regulators 1%.Embodiment 5:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 64%, temperature sensing polymer 15%, biomacromolecule 15%, cell growth factor and nutrition composition 3%, Photoepolymerizationinitiater initiater 1%, cross-linking agent 1%, other regulators 1%.Embodiment 6:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 50%, temperature sensing polymer 30%, biomacromolecule 12%, cell growth factor and nutrition composition 3%, Photoepolymerizationinitiater initiater 2%, cross-linking agent 2%, other regulators 1%.Embodiment 7:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 50%, temperature sensing polymer 14%, biomacromolecule 30%, cell growth factor and nutrition composition 3%, Photoepolymerizationinitiater initiater 1%, cross-linking agent 1%, other regulators 1%.Embodiment 8:
The quality percentage composition proportioning of 3D biometric print hydrogel material is:
Water 64%, temperature sensing polymer 15%, biomacromolecule 15%, cell growth factor and nutrition composition 3%, cross-linking agent 2%, other regulators 1%.
The preparation method of embodiment 1-8 is mix homogeneously under room temperature.
The hydrogel material of embodiment 1-8 is applied to all energy molding in 3D biometric print, and after molding, there is good biological property.
Claims (7)
1. a 3D biometric print hydrogel material, is characterized in that containing cell growth factor and nutrition composition.
2. a kind of 3D biometric print hydrogel material according to claim 1, is characterized in that containing water, temperature sensing polymer and biomacromolecule.
3. a kind of 3D biometric print hydrogel material according to claim 2, is characterized in that the mass percent of described each component is:
4. a kind of 3D biometric print hydrogel material according to claim 3, is characterized in that also containing Photoepolymerizationinitiater initiater, cross-linking agent and other regulators.
5. according to a kind of 3D biometric print hydrogel material described in any one in claim 2-4, it is characterized in that described temperature sensing polymer is selected from poly-N-isopropyl propyl amides class, PEO-PPO-PEO triblock copolymer and has the modified natural polymer of warm degeneration.
6. according to a kind of 3D biometric print hydrogel material described in any one in claim 2-4, it is characterized in that described biomacromolecule is selected from cellulose, hyaluronic acid, chitosan, Sargassum acids natural polymer and modifier thereof.
7. a 3D biometric print hydrogel material claimed in claim 1 application in 3D biometric print.
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| CN201410363077.5A CN104107457A (en) | 2014-05-28 | 2014-07-28 | 3D (three-dimensional) bio-printing hydrogel material and application thereof |
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| CN201410231198.4 | 2014-05-28 | ||
| CN201410231198.4A CN103977453A (en) | 2014-05-28 | 2014-05-28 | 3D (three-dimensional) bioprinting hydrogel material and application thereof |
| CN201410363077.5A CN104107457A (en) | 2014-05-28 | 2014-07-28 | 3D (three-dimensional) bio-printing hydrogel material and application thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104628936A (en) * | 2015-02-25 | 2015-05-20 | 湖北工业大学 | Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing |
| CN106008850A (en) * | 2016-06-08 | 2016-10-12 | 暨南大学 | Modified hydrogel material used for 3D printing and application of same to drug loading |
| CN106581762A (en) * | 2016-12-09 | 2017-04-26 | 杭州铭众生物科技有限公司 | 3D printing bio-ink and preparation method thereof, and 3D printing molding method |
| CN106902388A (en) * | 2015-12-22 | 2017-06-30 | 上海邦中高分子材料有限公司 | A kind of 3D printing biochargeable paper and its production and use |
| CN107523136A (en) * | 2016-12-09 | 2017-12-29 | 杭州铭众生物科技有限公司 | The degradable 3D printing bio-ink of temperature-responsive and 3D printing method |
| CN107698919A (en) * | 2017-10-31 | 2018-02-16 | 陕西爱骨医疗股份有限公司 | A kind of 3D printing bio-flexible material and preparation method thereof |
| CN108359143A (en) * | 2018-01-04 | 2018-08-03 | 艾伯尔三氐打印技术（重庆）有限公司 | A kind of hydrogel 3D printing material and preparation method thereof |
| CN109385140A (en) * | 2018-10-12 | 2019-02-26 | 深圳市康杰尔生物科技有限公司 | A kind of 3D biometric print nano-cellulose hydrogel ink |
| CN111070673A (en) * | 2019-12-24 | 2020-04-28 | 广东工业大学 | Method for 3D printing of polymer in condensed state |
| CN112250498A (en) * | 2020-09-28 | 2021-01-22 | 湖北富邦科技股份有限公司 | Hydrogel plant nutrition composition and preparation method thereof |
| CN113892481A (en) * | 2020-06-22 | 2022-01-07 | 苏州诺普再生医学有限公司 | Hydrogel for storage, transportation and bioprinting of cells or tissues |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104441654B (en) * | 2014-10-27 | 2016-08-24 | 清华大学深圳研究生院 | A kind of three dimensional biological printing equipment and method |
| CN105238132B (en) * | 2015-10-20 | 2018-07-31 | 中山大学 | A kind of bio-ink for 3D printing |
| CN105238690A (en) * | 2015-10-31 | 2016-01-13 | 林新颖 | Biological 3D cell planting shell printing wire as well as formula and manufacturing process thereof |
| US10738297B2 (en) | 2016-05-13 | 2020-08-11 | University Of Washington | 3D printable hydrogel materials |
| CN106237387A (en) * | 2016-08-24 | 2016-12-21 | 浙江省人民医院 | A kind of manufacture method of 3 D-printing bio-ink material |
| CN107418998B (en) * | 2016-09-14 | 2020-02-28 | 四川蓝光英诺生物科技股份有限公司 | Microparticle manipulation method, bioprinting method and biological construct |
| CN106378930B (en) * | 2016-09-20 | 2018-11-13 | 顺德职业技术学院 | The photocuring 3D printing method of temperature sensitive culture surface material |
| CN106474560B (en) * | 2016-11-04 | 2019-08-02 | 暨南大学 | A kind of hydrogel material and the preparation method and application thereof for 3D biometric print |
| US11530299B2 (en) * | 2016-12-30 | 2022-12-20 | Jf Polymers (Suzhou) Co., Ltd. | Biological ink |
| CN108098735B (en) * | 2017-12-12 | 2021-11-05 | 上海大学 | Biological micro-nano robot based on biological 3D printing and construction method thereof |
| CN109575683B (en) * | 2018-11-26 | 2021-11-16 | 西安理工大学 | Preparation method of hydrogel ink suitable for 3D bioprinting |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829361A (en) * | 2009-03-10 | 2010-09-15 | 广州迈普再生医学科技有限公司 | Nano-bionic material for tissue repair and preparation method thereof |
-
2014
- 2014-05-28 CN CN201410231198.4A patent/CN103977453A/en not_active Withdrawn
- 2014-07-28 CN CN201410363077.5A patent/CN104107457A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829361A (en) * | 2009-03-10 | 2010-09-15 | 广州迈普再生医学科技有限公司 | Nano-bionic material for tissue repair and preparation method thereof |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104628936A (en) * | 2015-02-25 | 2015-05-20 | 湖北工业大学 | Method for preparing high-strength double-network hydrogel stent by virtue of 3D printing |
| CN106902388A (en) * | 2015-12-22 | 2017-06-30 | 上海邦中高分子材料有限公司 | A kind of 3D printing biochargeable paper and its production and use |
| CN106008850A (en) * | 2016-06-08 | 2016-10-12 | 暨南大学 | Modified hydrogel material used for 3D printing and application of same to drug loading |
| CN106581762A (en) * | 2016-12-09 | 2017-04-26 | 杭州铭众生物科技有限公司 | 3D printing bio-ink and preparation method thereof, and 3D printing molding method |
| CN107523136A (en) * | 2016-12-09 | 2017-12-29 | 杭州铭众生物科技有限公司 | The degradable 3D printing bio-ink of temperature-responsive and 3D printing method |
| CN107698919A (en) * | 2017-10-31 | 2018-02-16 | 陕西爱骨医疗股份有限公司 | A kind of 3D printing bio-flexible material and preparation method thereof |
| CN108359143A (en) * | 2018-01-04 | 2018-08-03 | 艾伯尔三氐打印技术（重庆）有限公司 | A kind of hydrogel 3D printing material and preparation method thereof |
| CN109385140A (en) * | 2018-10-12 | 2019-02-26 | 深圳市康杰尔生物科技有限公司 | A kind of 3D biometric print nano-cellulose hydrogel ink |
| CN111070673A (en) * | 2019-12-24 | 2020-04-28 | 广东工业大学 | Method for 3D printing of polymer in condensed state |
| CN113892481A (en) * | 2020-06-22 | 2022-01-07 | 苏州诺普再生医学有限公司 | Hydrogel for storage, transportation and bioprinting of cells or tissues |
| CN112250498A (en) * | 2020-09-28 | 2021-01-22 | 湖北富邦科技股份有限公司 | Hydrogel plant nutrition composition and preparation method thereof |
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| CN103977453A (en) | 2014-08-13 |
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Application publication date: 20141022 |