CN104103847B - Single-double chamber coupling MFC (microbial fuel cell) stack and self-regulation method of PH value - Google Patents

Single-double chamber coupling MFC (microbial fuel cell) stack and self-regulation method of PH value Download PDF

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CN104103847B
CN104103847B CN201410327561.2A CN201410327561A CN104103847B CN 104103847 B CN104103847 B CN 104103847B CN 201410327561 A CN201410327561 A CN 201410327561A CN 104103847 B CN104103847 B CN 104103847B
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chamber
fuel cell
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CN104103847A (en
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李俊
邹文天
叶丁丁
朱恂
廖强
王宏
陈蓉
丁玉栋
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Chongqing University
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    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
    • H01M8/00—Fuel cells; Manufacture thereof
    • H01M8/16—Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01M—PROCESSES OR MEANS, e.g. BATTERIES, FOR THE DIRECT CONVERSION OF CHEMICAL ENERGY INTO ELECTRICAL ENERGY
    • H01M8/00—Fuel cells; Manufacture thereof
    • H01M8/04—Auxiliary arrangements, e.g. for control of pressure or for circulation of fluids
    • H01M8/04298—Processes for controlling fuel cells or fuel cell systems
    • H01M8/04694—Processes for controlling fuel cells or fuel cell systems characterised by variables to be controlled
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E60/00—Enabling technologies; Technologies with a potential or indirect contribution to GHG emissions mitigation
    • Y02E60/30—Hydrogen technology
    • Y02E60/50—Fuel cells

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Abstract

The invention discloses a single-double chamber coupling MFC (microbial fuel cell) stack and a self-regulation method of the PH value. The single-double chamber coupling MFC stack comprises n single-chamber MFCs and n double-chamber MFCs, and n is a natural number larger than or equal to 1. The single-double chamber coupling MFC stack is characterized in that each single-chamber MFC adopts an air cathode, and each double-chamber MFC adopts catholyte and is divided into a cathode chamber and an anode chamber through a proton exchange membrane; a waste liquor outlet of the anode chamber of the first double-chamber MFC is connected with a nutrient solution inlet of the first single-chamber MFC; and a waste liquor outlet of the first single-chamber MFC is connected with a nutrient solution inlet of the anode chamber of the second double-chamber MFC. The single-double chamber coupling MFC stack and the self-regulation method of the PH value can be widely applied to the fields of biology, energy, environment protection and the like, the problem of culture medium acidification is solved, and amplified stacking of the cell stack can be realized as required.

Description

Single dual chamber coupling microbiological fuel cell pile and the method for self-regulation pH value
Technical field
The present invention relates to Microbial fuel list Chi Dui, be specifically related to single dual chamber coupling Microbiological fuel cell pile and the method for self-regulation pH value.
Background technology
Microbiological fuel cell (MFC) can utilize the product electricity direct degradation of organic substances of bacterium to produce electricity Can be it is considered to be a kind of potential technology utilizing waste water to produce energy.But it is single The voltage of microbiological fuel cell is relatively low, needs to go here and there multiple Microbial fuel list ponds on circuit Connection, to improve voltage, meets practical application request.Meanwhile, single microbial fuel cell waste water Disposal ability is limited, needs to carry out multiple Microbial fuel list ponds connecting on water route, to promote Water treatment effect and wastewater treatment capacity.
Traditional microbiological fuel cell pile uses merely double-chamber microbiological fuel cell or single chamber Microbiological fuel cell is connected, but all Shortcomings:
During simple use double-chamber microbiological fuel cell composition pile, owing to adjacent cell shares electricity Solve liquid, easily cause electrolyte short circuit so that pile voltage severe attrition (Zhuang and Zhou 2009)。
During simple use double-chamber microbiological fuel cell composition pile, medium acidification can be caused. Owing to traditional double chamber microbiological fuel cell uses PEM to separate chamber, easily cause anode Proton accumulation (Rozendal, Hamelers, and Buisman2006).If culture medium PH buffer capacity is not enough, then anode can be caused to be acidified, and affects anode and produces electricity bacterium metabolic activity (Puig et al.2010).Connecting in multiple galvanic anode water routes, necessarily causes acidifying problem to be aggravated.And The unfavorable service conditions such as anode acidifying, culture medium shortage lure that again reverse electrode phenomena occurs in pile into, limit The output (Oh and Logan2007) of pile processed.Therefore some scholars use artificial tune Joint pH method (Zhuang et al.2012) (Zhang et al.2013), but this side Medium pH can not be continuously adjusted by method, and needs to expend extra manpower.Some are learned Person attempts to use high concentration buffer solution to solve medium acidification problem (Fan, Hu, and Liu2007), but high concentration buffer solution not only price is high, and environment itself can be caused dirty Dye.The growth of strengthening methanogen even can be competed, shadow by partial buffer solution with producing electricity bacterium Ring and produce electricity bacterium metabolism (Fan, Hu, and Liu2007).Recently, some scholars propose to use The method of anode and cathode solution circulation stops anolyte acidifying (Freguia et al.2008), But this method easily causes the biomembranous formation of cathode chamber, formed and block, be also unfavorable for making With more efficient electrolyte.
Therefore, medium acidification problem hinders the amplification stacking of microbiological fuel cell pile, shadow Ring the actual application of microbiological fuel cell.
Summary of the invention
The technical problem to be solved is to provide microbiological fuel cell pile and self-regulated The method of joint pH value, is automatically adjusted realizing pH value, thus promotes microbiological fuel cell pile Performance.
In order to solve above-mentioned technical problem, first technical scheme of the present invention is, including as follows Step:
A, making n are only with the single-chamber microbial fuel cell of air cathode；And set in battery chamber Put culture fluid import and waste liquid outlet；N takes >=natural number of 1；
B, making n separate cathode chamber and anode cavities only with catholyte and with PEM The double-chamber microbiological fuel cell of room；And in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
C, by dirty by activity to n single-chamber microbial fuel cell and n double-chamber microbiological fuel cell Mud is inoculated, and enables to produce continuous current；
D, the culture fluid import of first double-chamber microbiological fuel cell anode chamber is connected culture medium Memorizer, the waste liquid outlet of first double-chamber microbiological fuel cell anode chamber and first list The culture fluid import of chamber microbiological fuel cell connects；First single-chamber microbial fuel cell The culture fluid import of waste liquid outlet and second double-chamber microbiological fuel cell anode chamber connects； The waste liquid outlet of second double-chamber microbiological fuel cell anode chamber and second micro-life of single chamber The culture fluid import of thing fuel cell connects；The like；N-th two-chamber microbial fuel electricity The culture fluid import of the waste liquid outlet of pond anode chamber and n-th single-chamber microbial fuel cell Connect；The electrolyte inlet of the cathode chamber of all double-chamber microbiological fuel cells is simultaneously connected with electricity Solving liquid memorizer, the waste liquid outlet of the cathode chamber of all double-chamber microbiological fuel cells connects useless Liquid bath；
E, culture medium is used the continuous batch of circulation or continuously stream mode inject, culture medium will flow sequentially through First double-chamber microbiological fuel cell anode chamber, first single-chamber microbial fuel cell chamber Room, second double-chamber microbiological fuel cell anode chamber, second single-chamber microbial fuel electricity Cell cavity room ... n-th double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial Fuel cell chamber；
F, the electrolyte inlet injection two-chamber microbial fuel electricity that catholyte is arranged by cathode chamber The cathode chamber in pond.
The principle of the method for microbiological fuel cell pile self-regulation pH value of the present invention is: In the present invention in single-chamber microbial fuel cell negative electrode biomembrane due near air, there occurs as Lower reaction:
CH3COO-+H++2O2→2CO2+2H2O
Understanding in single-chamber microbial fuel cell, the consumption of 1mol acetate is along with 1mol Hydrionic consumption, this shows that in single-chamber microbial fuel cell, degraded culture medium produces the reaction of electricity It is accompanied by what hydrion consumed.Therefore, double-chamber microbiological fuel cell chamber accumulation hydrogen from Son is consumed in single-chamber microbial fuel cell, so that culture medium keeps neutrality, it is achieved that PH continuously adjusts.
And the PEM of double-chamber microbiological fuel cell increases two adjacent microorganism combustions Ionic conduction resistance between material battery, it is to avoid the generation of electrolyte short circuit between adjacent cell.
Second technical scheme of the present invention is, single dual chamber coupling microbiological fuel cell pile, bag Include n single-chamber microbial fuel cell and n double-chamber microbiological fuel cell；N takes >=1 Natural number；It is characterized in that:
Described single-chamber microbial fuel cell is the single-chamber microbial fuel using air cathode Battery, and culture fluid import and waste liquid outlet are set in battery chamber；Described double-chamber microbiological Fuel cell is for using catholyte and separating cathode chamber and anode cavities with PEM The double-chamber microbiological fuel cell of room, and in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
The culture fluid import of first double-chamber microbiological fuel cell anode chamber connects culture medium Memorizer, the waste liquid outlet of first double-chamber microbiological fuel cell anode chamber and first list The culture fluid import of chamber microbiological fuel cell connects；First single-chamber microbial fuel cell The culture fluid import of waste liquid outlet and second double-chamber microbiological fuel cell anode chamber connects； The waste liquid outlet of second double-chamber microbiological fuel cell anode chamber and second micro-life of single chamber The culture fluid import of thing fuel cell connects；The like；N-th two-chamber microbial fuel electricity The culture fluid import of the waste liquid outlet of pond anode chamber and n-th single-chamber microbial fuel cell Connect；The electrolyte inlet of the cathode chamber of all double-chamber microbiological fuel cells is simultaneously connected with electricity Solving liquid memorizer, the waste liquid outlet of the cathode chamber of all double-chamber microbiological fuel cells connects useless Liquid bath；
Culture medium use the continuous batch of circulation or continuously stream mode inject, culture medium will flow sequentially through the One double-chamber microbiological fuel cell anode chamber, first single-chamber microbial fuel cell chamber Room, second double-chamber microbiological fuel cell anode chamber, second single-chamber microbial fuel electricity Cell cavity room ... n-th double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial Fuel cell chamber；The electrolyte inlet that catholyte is arranged by cathode chamber injects the micro-life of dual chamber The cathode chamber of thing fuel cell；
N single-chamber microbial fuel cell electrode and the electrode of n double-chamber microbiological fuel cell Series, parallel or series-parallel connection is used to connect.
The present invention proposes to coordinate single-chamber microbial fuel cell with double-chamber microbiological fuel cell Composition pile, in pile, the anolyte of double-chamber microbiological fuel cell is through single-chamber microbial fuel electricity It is neutral that pond is adjusted to pH so that subordinate's microbiological fuel cell is not affected by medium acidification. The existence in two-chamber microbial fuel list pond avoids the generation of electrolyte short circuit.Single-chamber microbial fires Material battery coordinates with double-chamber microbiological fuel cell, maintains higher output, improves The overall performance of pile.
Single dual chamber of the present invention coupling microbiological fuel cell pile and the side of self-regulation pH value Method provides the benefit that: in pile of the present invention, the anolyte of double-chamber microbiological fuel cell is through single chamber It is neutral that microbiological fuel cell is adjusted to pH so that subordinate's microbiological fuel cell is not cultivated Base acidifying impact；And single-chamber microbial fuel cell is joined with double-chamber microbiological fuel cell Close, maintain higher output, improve the overall performance of pile；The present invention solves Medium acidification problem, microbiological fuel cell pile can amplify stacking as required；Can extensively answer For fields such as biology, the energy, environmental protection, have a good application prospect.
Accompanying drawing explanation
Fig. 1 is structure and the electrode connection diagram of embodiment 4.
Fig. 2 is electrode hybrid connected structure schematic diagram.
Fig. 3 is discharge curve, pH variation diagram and the acetic acid using the method for embodiment 1 to obtain Sodium variation diagram.
Fig. 4 is the power curve of the pile of embodiment 4.
Fig. 5 is the polarization curve of the pile of embodiment 4.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further described specifically, but the enforcement of the present invention Mode is not limited to this.
Embodiment 1: the method for microbiological fuel cell pile self-regulation pH value, it is characterised in that: Comprise the steps:
A, one single-chamber microbial fuel cell of making, this single-chamber microbial fuel cell negative electrode is single The carbon cloth that face is modified with platinum and hydrophobic processes, catalyst made from platonic is facing to intermediate cavity, another side Towards air；And culture fluid import and waste liquid outlet are set in battery chamber；
B, making one separate cathode chamber and anode cavities only with catholyte and with PEM The double-chamber microbiological fuel cell of room；And in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
C, single-chamber microbial fuel cell and double-chamber microbiological fuel cell use activated sludge connect Kind, make it possible to produce continuous current；
D, assembling microbiological fuel cell pile: by the cultivation of double-chamber microbiological fuel cell anode chamber Liquid import connects culture medium memorizer, the waste liquid outlet of double-chamber microbiological fuel cell anode chamber It is connected with the culture fluid import of single-chamber microbial fuel cell；Giving up of single-chamber microbial fuel cell Liquid outlet connects the culture fluid import of double-chamber microbiological fuel cell anode chamber；Double-chamber microbiological The electrolyte inlet of the cathode chamber of fuel cell connects electrolyte reservoir, and double-chamber microbiological fires The waste liquid outlet of the cathode chamber of material battery connects waste liquid tank；And by double-chamber microbiological fuel cell Electrode is connected in parallel with single-chamber microbial fuel cell electrode；
E, culture medium are injected with the continuous batch of circulation, make culture medium flow successively through two-chamber microbial fuel electricity Pond anode chamber and single-chamber microbial fuel cell chamber；Medium component is Na2HPO4·12H2O:15.35g/L,KH2PO4:3g/L,CH3COONa·3H2O: 10.14g/L,NaCl:0.5g/L,NH4Cl:0.1g/L, MgSO4·7H2O:0.1g/L,CaCl2:11.327mg/L；
F, being stored in electrolyte reservoir by catholyte, the electrolyte arranged by cathode chamber is entered Mouth injects the cathode chamber of double-chamber microbiological fuel cell, and catholyte is 30mM L-1Ferrum cyanogen Change potassium solution.
Observe pile operational factor, see Fig. 3, in pile running, although pH value is slightly There is decline, but final stable about 6.8.Further, running can keep 0.5V Above regulated output voltage.
From fig. 3 it can also be seen that use the pile that dual chamber-double-chamber microbiological fuel cell is constituted Voltage then continuous decrease, it is impossible to keep stable.And use single chamber-single-chamber microbial fuel electricity Although the pile that pond is constituted can keep pH stable about 7.5, but output voltage only has real Execute the 80% of example 1.
Embodiment 2: the method for microbiological fuel cell pile self-regulation pH value, including walking as follows Rapid:
A, two single-chamber microbial fuel cells of making, this single-chamber microbial fuel cell negative electrode is single The carbon paper that face is modified with platinum and hydrophobic processes, catalyst made from platonic is facing to intermediate cavity, another side Towards air；And culture fluid import and waste liquid outlet are set in battery chamber；
B, make two again and separate cathode chamber and anode only with catholyte and with PEM The double-chamber microbiological fuel cell of chamber；And in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
C, by single-chamber microbial fuel cell with use double-chamber microbiological fuel cell use activated sludge Inoculation, makes it possible to produce continuous current；
D, assembling microbiological fuel cell pile: will first double-chamber microbiological fuel cell anode cavities The culture fluid import of room connects culture medium memorizer, first double-chamber microbiological fuel cell anode The culture fluid import of the waste liquid outlet of chamber and first single-chamber microbial fuel cell connects；The The waste liquid outlet of one single-chamber microbial fuel cell and second double-chamber microbiological fuel cell The culture fluid import of anode chamber connects；Second double-chamber microbiological fuel cell anode chamber The culture fluid import of waste liquid outlet and second single-chamber microbial fuel cell connects, second list The waste liquid outlet of chamber microbiological fuel cell connects waste liquid tank；All double-chamber microbiological fuel cells The electrolyte inlet of cathode chamber be simultaneously connected with electrolyte reservoir, all double-chamber microbiologicals fire The waste liquid outlet of the cathode chamber of material battery connects waste liquid tank；And by four microbiological fuel cells Electrode adopt and be connected serially；
E, culture medium is injected with continuous stream mode, make culture medium flow successively through first micro-life of dual chamber Thing anode of fuel cell chamber, first single-chamber microbial fuel cell chamber, second dual chamber Anode of microbial fuel cell chamber and second single-chamber microbial fuel cell chamber, then pass through Second single-chamber microbial fuel cell waste liquid outlet is discharged；Medium component is Na2HPO4·12H2O:8.35g/L,KH2PO4:2g/L,CH3COONa·3H2O:8.14g/L, NaCl:0.75g/L,NH4Cl:0.2g/L,MgSO4·7H2O:0.2g/L,CaCl2:13.327mg/L；
F, the electrolyte inlet injection two-chamber microbial fuel electricity that catholyte is arranged by cathode chamber The cathode chamber in pond, catholyte is 40mM L-1Potassium sulfite solution.
Embodiment 3: the method for microbiological fuel cell pile self-regulation pH value, including walking as follows Rapid:
A, making n are only with the single-chamber microbial fuel cell of air cathode；The micro-life of described single chamber Thing fuel cell be one side platinum modify and hydrophobic process carbon paper, catalyst made from platonic facing in Between chamber, another side is towards air；And culture fluid import and waste liquid outlet are set in battery chamber； N takes >=natural number of 3；
B, making n separate cathode chamber and anode cavities only with catholyte and with PEM The double-chamber microbiological fuel cell of room；And in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
C, by dirty by activity to n single-chamber microbial fuel cell and n double-chamber microbiological fuel cell Mud is inoculated, and makes it possible to produce continuous current；
D, assembling microbiological fuel cell pile: by first double-chamber microbiological fuel cell anode chamber Culture fluid import connect culture medium memorizer, first double-chamber microbiological fuel cell anode cavities The culture fluid import of the waste liquid outlet of room and first single-chamber microbial fuel cell connects；First The waste liquid outlet of single-chamber microbial fuel cell and second double-chamber microbiological fuel cell sun The culture fluid import of pole chamber connects；Giving up of second double-chamber microbiological fuel cell anode chamber Liquid outlet is connected with the culture fluid import of second single-chamber microbial fuel cell；The like； The waste liquid outlet of n-th double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial The culture fluid import of fuel cell connects, the waste liquid outlet of n-th single-chamber microbial fuel cell Connect waste liquid tank；The electrolyte inlet of the cathode chamber of all double-chamber microbiological fuel cells is simultaneously Connect electrolyte reservoir, the waste liquid outlet of the cathode chamber of all double-chamber microbiological fuel cells Connect waste liquid tank；And by every single-chamber microbial fuel cell electrode respectively with a micro-life of dual chamber The electrode of thing fuel cell is in parallel, then the electrode series connection after being connected in parallel, and i.e. uses series-parallel connection Connect；Electrode connection diagram is shown in Fig. 2；
E, culture medium using continuous stream mode inject, it is micro-that culture medium will flow sequentially through first dual chamber Biological fuel cell anode chamber, first single-chamber microbial fuel cell chamber, second double Chamber microbiological fuel cell anode chamber, second single-chamber microbial fuel cell chamber ... the N double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial fuel cell chamber Room, then discharged by n-th single-chamber microbial fuel cell waste liquid outlet；
F, electrolyte inlet injection all double-chamber microbiologicals combustion that catholyte is arranged by cathode chamber The cathode chamber of material battery.
In a particular embodiment, described medium component includes: Na2HPO4·12H2O:2.56-15.35g/L,KH2PO4:0.5-3g/L,CH3COONa·3H2O: 2.25-10.14g/L,NaCl:0.5-1g/L,NH4Cl:0.1-0.3g/L,MgSO4·7H2O:0.1-0.3 g/L,CaCl2:11.327-15mg/L。
Described catholyte can be 20～60mM L-1Potassium ferricyanide solution or concentration be 20～60mM L-1Potassium sulfite solution.
In a particular embodiment, catholyte can also add pH be 4.8, concentration be 40～80 mM·L-1Phosphate slow releasing agent, the phosphate slow releasing agent added and potassium ferricyanide solution or Asia The ratio of potassium sulfate solution is 4:1.
Embodiment 4: see Fig. 1, single dual chamber coupling microbiological fuel cell pile, adopted by two Single-chamber microbial fuel cell and two with air cathode only with catholyte and use proton Exchange membrane separates the double-chamber microbiological fuel cell of cathode chamber and anode chamber and constitutes, wherein, This single-chamber microbial fuel cell negative electrode is the carbon cloth that one side platinum is modified and hydrophobic processes, platiniferous Catalyst face is towards intermediate cavity, and another side is towards air；And culture fluid is set in battery chamber Import and waste liquid outlet；The anode chamber of double-chamber microbiological fuel cell arrange culture fluid import and Waste liquid outlet, cathode chamber arranges electrolyte inlet and electrolyte outlet；
Described single-chamber microbial fuel cell and double-chamber microbiological fuel cell use activity dirty Mud is inoculated, and enables to produce continuous current；
The culture fluid import of just first double-chamber microbiological fuel cell anode chamber connects training Support base memorizer, the waste liquid outlet of first double-chamber microbiological fuel cell anode chamber and first The culture fluid import of single-chamber microbial fuel cell connects；First single-chamber microbial fuel electricity The culture fluid import of the waste liquid outlet in pond and second double-chamber microbiological fuel cell anode chamber Connect；The waste liquid outlet of second double-chamber microbiological fuel cell anode chamber and second single chamber The culture fluid import of microbiological fuel cell connects；Giving up of second single-chamber microbial fuel cell Liquid outlet connects waste liquid tank；The electrolyte of the cathode chamber of all double-chamber microbiological fuel cells enters Mouth is simultaneously connected with electrolyte reservoir, giving up of the cathode chamber of all double-chamber microbiological fuel cells Liquid outlet connects waste liquid tank；
The electrode of four microbiological fuel cells uses series-parallel connection mode to connect, and i.e. first dual chamber is micro- Biological fuel cell and first single-chamber microbial fuel cell electrode are connected in parallel, and second double Chamber microbiological fuel cell and second single-chamber microbial fuel cell electrode are connected in parallel, in parallel After electrode connect again；Culture medium is injected with continuous stream mode, makes culture medium flow successively through One double-chamber microbiological fuel cell anode chamber, first single-chamber microbial fuel cell chamber Room, second double-chamber microbiological fuel cell anode chamber and second single-chamber microbial fuel electricity Cell cavity room, then discharged by second single-chamber microbial fuel cell waste liquid outlet；
Medium component is Na2HPO4·12H2O:6.35g/L,KH2PO4:1g/L, CH3COONa·3H2O:10.14g/L, NaCl:0.5g/L, NH4Cl:0.3g/L, MgSO4·7H2O:0.3g/L,CaCl2:15.327mg/L；
By the electrolyte inlet that cathode chamber is arranged, catholyte is injected all double-chamber microbiologicals fire The cathode chamber of material battery；Catholyte is 40mM L-1Potassium ferricyanide solution, at catholyte In be additionally added pH be 4.8, concentration be 60mM L-1Phosphate slow releasing agent, the phosphoric acid added Salt slow releasing agent is 4:1 with the ratio of potassium ferricyanide solution or potassium sulfite solution.
Observing pile operational factor, see Fig. 4, Fig. 5, in pile running, pile is High-power for 5.21mW. from Fig. 4 it can also be seen that embodiment 4 than use four dual chamber micro- The peak power of the pile that biological fuel cell is constituted is high by 44%, and ratio uses four single-chamber microbials The peak power of the pile that fuel cell is constituted is high by 56%.
In example 4, it is also possible to as required the electrode of microbiological fuel cell is connected or It is connected in parallel.
Embodiment 5: single dual chamber coupling microbiological fuel cell pile, including n single-chamber microbial Fuel cell and n double-chamber microbiological fuel cell；N takes >=natural number of 1；Its feature It is:
Described single-chamber microbial fuel cell is the single-chamber microbial fuel using air cathode Battery, and culture fluid import and waste liquid outlet are set in battery chamber；Described double-chamber microbiological Fuel cell is for using catholyte and separating cathode chamber and anode cavities with PEM The double-chamber microbiological fuel cell of room, and in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
The culture fluid import of first double-chamber microbiological fuel cell anode chamber connects culture medium Memorizer, the waste liquid outlet of first double-chamber microbiological fuel cell anode chamber and first list The culture fluid import of chamber microbiological fuel cell connects；First single-chamber microbial fuel cell The culture fluid import of waste liquid outlet and second double-chamber microbiological fuel cell anode chamber connects； The waste liquid outlet of second double-chamber microbiological fuel cell anode chamber and second micro-life of single chamber The culture fluid import of thing fuel cell connects；The like；N-th two-chamber microbial fuel electricity The culture fluid import of the waste liquid outlet of pond anode chamber and n-th single-chamber microbial fuel cell Connect；The electrolyte inlet of the cathode chamber of all double-chamber microbiological fuel cells is simultaneously connected with electricity Solving liquid memorizer, the waste liquid outlet of the cathode chamber of all double-chamber microbiological fuel cells connects useless Liquid bath；
Culture medium use the continuous batch of circulation or continuously stream mode inject, culture medium will flow sequentially through the One double-chamber microbiological fuel cell anode chamber, first single-chamber microbial fuel cell chamber Room, second double-chamber microbiological fuel cell anode chamber, second single-chamber microbial fuel electricity Cell cavity room ... n-th double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial Fuel cell chamber；The electrolyte inlet that catholyte is arranged by cathode chamber injects the micro-life of dual chamber The cathode chamber of thing fuel cell；
Single-chamber microbial fuel cell electrode uses string with the electrode of double-chamber microbiological fuel cell Connection, in parallel or series-parallel connection connection；
In a particular embodiment, described medium component includes: Na2HPO4·12H2O:2.56-15.35g/L,KH2PO4:0.5-3g/L,CH3COONa·3H2O: 2.25-10.14g/L,NaCl:0.5-1g/L,NH4Cl:0.1-0.3g/L,MgSO4·7H2O:0.1-0.3 g/L,CaCl2:11.327-15mg/L。
Described catholyte can be 20～60mM L-1Potassium ferricyanide solution or concentration be 20～60mM L-1Potassium sulfite solution.
In a particular embodiment, catholyte can also add pH be 4.8, concentration be 40～80 mM·L-1Phosphate slow releasing agent, the phosphate slow releasing agent added and potassium ferricyanide solution or Asia The ratio of potassium sulfate solution is 4:1.

Claims (8)

1. the method for microbiological fuel cell pile self-regulation pH value, it is characterised in that: include as follows Step:
A, making n are only with the single-chamber microbial fuel cell of air cathode；And set in battery chamber Put culture fluid import and waste liquid outlet；N takes >=natural number of 2；
B, making n separate cathode chamber and anode cavities only with catholyte and with PEM The double-chamber microbiological fuel cell of room；And in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
C, by dirty by activity to n single-chamber microbial fuel cell and n double-chamber microbiological fuel cell Mud is inoculated, and enables to produce continuous current；
D, the culture fluid import of first double-chamber microbiological fuel cell anode chamber is connected culture medium Memorizer, the waste liquid outlet of first double-chamber microbiological fuel cell anode chamber and first list The culture fluid import of chamber microbiological fuel cell connects；First single-chamber microbial fuel cell The culture fluid import of waste liquid outlet and second double-chamber microbiological fuel cell anode chamber connects； The waste liquid outlet of second double-chamber microbiological fuel cell anode chamber and second micro-life of single chamber The culture fluid import of thing fuel cell connects；The like；N-th two-chamber microbial fuel electricity The culture fluid import of the waste liquid outlet of pond anode chamber and n-th single-chamber microbial fuel cell Connect；The electrolyte inlet of the cathode chamber of all double-chamber microbiological fuel cells is simultaneously connected with electricity Solving liquid memorizer, the waste liquid outlet of the cathode chamber of all double-chamber microbiological fuel cells connects useless Liquid bath；Further, by the electrode of single-chamber microbial fuel cell and double-chamber microbiological fuel cell After electrode series, parallel or series-parallel connection connect, the more single dual chamber coupling Microbial fuel electricity that will obtain The electrode of Chi Dui is connected with outer load；
E, culture medium is used the continuous batch of circulation or continuously stream mode inject, culture medium will flow sequentially through First double-chamber microbiological fuel cell anode chamber, first single-chamber microbial fuel cell chamber Room, second double-chamber microbiological fuel cell anode chamber, second single-chamber microbial fuel electricity Cell cavity room ... n-th double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial Fuel cell chamber；
F, the electrolyte inlet injection two-chamber microbial fuel electricity that catholyte is arranged by cathode chamber The cathode chamber in pond.
The method of microbiological fuel cell pile self-regulation pH value the most according to claim 1, its It is characterised by: described medium component includes: Na2HPO4·12H2O:2.56-15.35g/L, KH2PO4:0.5-3g/L,CH3COONa·3H2O:2.25-10.14g/L, NaCl:0.5-1g/L, NH4Cl:0.1-0.3g/L,MgSO4·7H2O:0.1-0.3g/L,CaCl2:11.327-15mg/L。
The side of microbiological fuel cell pile self-regulation pH value the most according to claim 1 and 2 Method, it is characterised in that: described catholyte is 20～60mM L-1Potassium ferricyanide solution or Concentration is 20～60mM L-1Potassium sulfite solution.
The method of microbiological fuel cell pile self-regulation pH value the most according to claim 3, its Be characterised by: be additionally added in catholyte pH be 4.8, concentration be 40～80mM L-1Phosphoric acid Salt slow releasing agent, the phosphate slow releasing agent added and potassium ferricyanide solution or potassium sulfite solution Ratio is 4:1.
5. single dual chamber coupling microbiological fuel cell pile, including n single-chamber microbial fuel cell and N double-chamber microbiological fuel cell；N takes >=natural number of 2；It is characterized in that:
Described single-chamber microbial fuel cell is the single-chamber microbial fuel using air cathode Battery, and culture fluid import and waste liquid outlet are set in battery chamber；Described double-chamber microbiological Fuel cell is for using catholyte and separating cathode chamber and anode cavities with PEM The double-chamber microbiological fuel cell of room, and in anode chamber culture fluid import is set and waste liquid goes out Mouthful, in cathode chamber, electrolyte inlet and electrolyte outlet are set；
The culture fluid import of first double-chamber microbiological fuel cell anode chamber connects culture medium Memorizer, the waste liquid outlet of first double-chamber microbiological fuel cell anode chamber and first list The culture fluid import of chamber microbiological fuel cell connects；First single-chamber microbial fuel cell The culture fluid import of waste liquid outlet and second double-chamber microbiological fuel cell anode chamber connects； The waste liquid outlet of second double-chamber microbiological fuel cell anode chamber and second micro-life of single chamber The culture fluid import of thing fuel cell connects；The like；N-th two-chamber microbial fuel electricity The culture fluid import of the waste liquid outlet of pond anode chamber and n-th single-chamber microbial fuel cell Connect；The electrolyte inlet of the cathode chamber of all double-chamber microbiological fuel cells is simultaneously connected with electricity Solving liquid memorizer, the waste liquid outlet of the cathode chamber of all double-chamber microbiological fuel cells connects useless Liquid bath；
Culture medium use the continuous batch of circulation or continuously stream mode inject, culture medium will flow sequentially through the One double-chamber microbiological fuel cell anode chamber, first single-chamber microbial fuel cell chamber Room, second double-chamber microbiological fuel cell anode chamber, second single-chamber microbial fuel electricity Cell cavity room ... n-th double-chamber microbiological fuel cell anode chamber and n-th single-chamber microbial Fuel cell chamber；The electrolyte inlet that catholyte is arranged by cathode chamber injects the micro-life of dual chamber The cathode chamber of thing fuel cell；
N single-chamber microbial fuel cell electrode and the electrode of n double-chamber microbiological fuel cell Series, parallel or series-parallel connection is used to connect.
Single dual chamber the most according to claim 5 coupling microbiological fuel cell pile, its feature exists In: described medium component includes: Na2HPO4·12H2O:2.56-15.35g/L, KH2PO4:0.5-3g/L,CH3COONa·3H2O:2.25-10.14g/L, NaCl:0.5-1g/L, NH4Cl:0.1-0.3g/L,MgSO4·7H2O:0.1-0.3g/L,CaCl2:11.327-15mg/L。
7. couple microbiological fuel cell pile, its feature according to the single dual chamber described in claim 5 or 6 It is: described catholyte is 20～60mM L-1Potassium ferricyanide solution or concentration be 20～ 60mM·L-1Potassium sulfite solution.
Single dual chamber the most according to claim 7 coupling microbiological fuel cell pile, its feature exists In: be additionally added in catholyte pH be 4.8, concentration be 40～80mM L-1Phosphate slow release Agent, the phosphate slow releasing agent added with the ratio of potassium ferricyanide solution or potassium sulfite solution is 4:1。
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