CN104096510A - Method for uniformly blending components of culture medium by employing fluid bed drying technology - Google Patents
Method for uniformly blending components of culture medium by employing fluid bed drying technology Download PDFInfo
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- CN104096510A CN104096510A CN201310126681.1A CN201310126681A CN104096510A CN 104096510 A CN104096510 A CN 104096510A CN 201310126681 A CN201310126681 A CN 201310126681A CN 104096510 A CN104096510 A CN 104096510A
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- culture medium
- feed bin
- material bin
- materials
- bed drying
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Abstract
The invention relates to a method for uniformly blending components of a culture medium by employing fluid bed drying technology. The method includes following steps: (1) feeding air, after being filtered, to a bottom cylinder from a bottom of a fluid bed drying machine so that air flows into a material bin and uniformly blows main materials in the culture medium to form a continuously fluidizing status; (2) dissolving micro-scale materials in the culture medium in water, spraying the dissolved micro-scale materials into the material bin so that the dissolved micro-scale materials are atomized under an effect of compressed air and are sprayed on surfaces of the main materials; (3) fully blending the main materials and the micro-scale materials in the material bin to form master batches which grows gradually to form granules which conform to a requirement; and (4) exhausting gas at the top of the material bin so that granules in the material bin are dried due to evaporation of moisture, thereby being removed out from the material bin. By means of the method, materials in different contents can be mixed more uniformly, quality of the culture medium is more homogeneous and differences among products in same batch are smaller. A finished product of the culture medium is granular in shape and is better in dissolving performance. Dust can be reduced when the finishing product is used and the culture medium is beneficial to health of a user.
Description
Technical field
The present invention relates to a kind of blending process, specifically a kind of technique of utilizing fluidized bed drying technology to mix medium component.
Background technology
When culture medium is prepared, very multicomponent ratio differs greatly, and what have even can be over 10000 times.In order to obtain the culture medium that quality is higher, the principal goods material and the trace material that form culture medium evenly need to be mixed.Prior art generally adopts machinery or manually mixes technology.Yet machinery or manually mix mixing between the material differing greatly for mentioned component ratio and cannot reach expection, cannot really mix completely.The uneven meeting that mixes of the principal goods material of culture medium and trace material produces the spotty problem of same batch products, and this is a comparatively serious problem for manufacturer.
The different culture media raw material that the object of the invention is to utilize fluidized bed drying technology to make content differ very greatly different evenly mixes, and for the problems referred to above, is good solutions.
Summary of the invention
The present invention cannot mix problem for solving above-mentioned culture medium prescription, aims to provide a kind of technique of utilizing fluidized bed drying technology to mix medium component.
In order to achieve the above object, the invention provides a kind of technique of utilizing fluidized bed drying technology to mix medium component, comprise the following steps:
Step 1: air draught imports end cylinder after filtering from fluidized bed drying motor spindle, air draught enters feed bin the principal goods material in culture medium is evenly blown afloat, and forms continuous fluidized state;
Step 2: the trace material in culture medium is dissolved in water, and injects feed bin by spray gun, atomization be ejected into material surface under compressed-air actuated effect;
Step 3: principal goods material and trace material fully mix in feed bin, and form master batch, grow up into gradually satisfactory particle;
Step 4: bleed in the top at feed bin, feed bin endoparticle is dried because moisture evaporates, and then is pulled out feed bin.
The present invention is with respect to prior art, can make the component of different content mix more evenly, culture medium quality homogeneous more; Less with the product differentiation in a collection of; Finished product culture medium is graininess, and solubility property is better; During use, also reduce dust, be of value to user's health.
The specific embodiment
Below in conjunction with embodiment, specific embodiment of the invention method is further described.This embodiment is for technical scheme of the present invention is more clearly described, and can not limit the scope of the invention with this.
Embodiment mono-:
D culture medium prescription table
| Component (principal goods material) | Content (mg/L) |
| CaCl 2(anhydrous) | 200 |
| Fe(NO 3) 3·9H 2O | 0.1 |
| KCL | 400 |
| MgSO 4(anhydrous) | 97.67 |
| NaH 2PO 4·H 2O | 125 |
| Phenol red | 15 |
| L-arginine HCl | 84 |
| CYSTINE 2HCl | 63 |
| Glu | 584 |
| Glycine | 30 |
| L-Histidine HClH 2O | 42 |
| ILE | 105 |
| L-Leu | 105 |
| 1B HCl | 146 |
| METHIONINE | 30 |
| L-Phe | 66 |
| Serine | 42 |
| L-threonine | 95 |
| L-Trp | 16 |
| TYR 2Na2H 2O | 104 |
| Valine | 94 |
| D-VB5 calcium | 4 |
| Choline Chloride | 4 |
| Folic acid | 4 |
| I-inositol | 7.2 |
| Niacinamide | 4 |
| Riboflavin | 0.4 |
| Thiamines HCl | 4 |
| Pyridoxol HC | 4 |
| Component (trace material) | Content (mg/L) |
| Copper sulphate | 0.001 |
As seen from the above table, the content of copper sulphate lower than 1,000,000 of whole compositions/.The present embodiment need to evenly mix trace material copper sulphate and principal goods material, and concrete steps are as follows:
Step 1: first the main material weighing preparation beyond copper sulphate is mixed, gross weight is 24.7537 kilograms, adds feed bin; Air draught imports end cylinder after filtering from fluidized bed drying motor spindle, and air draught enters feed bin the principal goods material in culture medium is evenly blown afloat, and forms continuous fluidized state;
Step 2: 10 milligrams of copper sulphate are dissolved in 1000mL pure water again, inject feed bin by spray gun, atomization is ejected into material surface under compressed air effect;
Step 3: principal goods material and trace material fully mix in feed bin, and form master batch, grow up into gradually satisfactory particle;
Step 4: after principal goods material and trace material fully mix, feed bin top vacuumizes, the moisture in the culture medium particle mixing evaporates and is drawn out of feed bin and collects.
From splendid attire mix culture medium bucket diverse location get three samples, by atomic absorption spectrophotometry, carry out copper sulphate and quantitatively detect.
Through converting, the ratio of copper sulphate in culture medium is respectively 0.4036ug/g, 0.4015ug/g, 0.4003ug/g, average out to 0.4018ug/g.
The present invention is with respect to prior art, can make the component of different content mix more evenly, culture medium quality homogeneous more; Less with the product differentiation in a collection of; Finished product culture medium is graininess, and solubility property is better; During use, also reduce dust, be of value to user's health.
Above are only the preferred embodiment of the present invention, be to be noted that for the ordinary skill technical staff in the industry, can be by some improvement and replacement under principle of the present invention, this improvement and replacement also should be considered as protection scope of the present invention.
Claims (1)
1. utilize fluidized bed drying technology to mix a technique for medium component, it is characterized in that having used fluid bed dryer, and comprise the following steps:
Step 1: air draught imports end cylinder after filtering from fluidized bed drying motor spindle, the feed bin that air draught enters fluid bed dryer evenly blows afloat the principal goods material in culture medium, forms continuous fluidized state;
Step 2: the trace material in culture medium is dissolved in water, and injects feed bin by spray gun, atomization be ejected into material surface under compressed-air actuated effect;
Step 3: principal goods material and trace material fully mix in feed bin, and form master batch, grow up into gradually satisfactory particle;
Step 4: bleed in the top at feed bin, feed bin endoparticle is dried because moisture evaporates, and then is pulled out feed bin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310126681.1A CN104096510A (en) | 2013-04-12 | 2013-04-12 | Method for uniformly blending components of culture medium by employing fluid bed drying technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310126681.1A CN104096510A (en) | 2013-04-12 | 2013-04-12 | Method for uniformly blending components of culture medium by employing fluid bed drying technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104096510A true CN104096510A (en) | 2014-10-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310126681.1A Pending CN104096510A (en) | 2013-04-12 | 2013-04-12 | Method for uniformly blending components of culture medium by employing fluid bed drying technology |
Country Status (1)
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| CN (1) | CN104096510A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108495926A (en) * | 2015-12-17 | 2018-09-04 | 生命技术公司 | Particle and preparation method thereof for cell culture |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308669A (en) * | 1998-04-30 | 2001-08-15 | Basf公司 | Dried microorganism cultures and method for producing same |
-
2013
- 2013-04-12 CN CN201310126681.1A patent/CN104096510A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1308669A (en) * | 1998-04-30 | 2001-08-15 | Basf公司 | Dried microorganism cultures and method for producing same |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108495926A (en) * | 2015-12-17 | 2018-09-04 | 生命技术公司 | Particle and preparation method thereof for cell culture |
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Address after: 201400 Shanghai city Fengxian District Road No. 269 Applicant after: Yuan Pei biotech inc, Shanghai Address before: 201400 Shanghai city Fengxian District Road No. 269 Applicant before: SHANGHAI BASAL MEDIA BIOTECHNOLOGY CO., LTD. |
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Free format text: CORRECT: APPLICANT; FROM: SHANGHAI BASAL MEDIA BIOTECHNOLOGY LTD. TO: SHANGHAI BASAL MEDIA BIOTECHNOLOGY CO., LTD. |
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Application publication date: 20141015 |