CN104095907A - Extracting method, detection method and application of total alkaloids in sophora tonkinensis - Google Patents
Extracting method, detection method and application of total alkaloids in sophora tonkinensis Download PDFInfo
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- CN104095907A CN104095907A CN201410371787.2A CN201410371787A CN104095907A CN 104095907 A CN104095907 A CN 104095907A CN 201410371787 A CN201410371787 A CN 201410371787A CN 104095907 A CN104095907 A CN 104095907A
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- chloroform
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Abstract
The invention discloses an extracting method, a detection method and application of total alkaloids in sophora tonkinensis. The extracting method comprises two ways: (1) conducting heating, backflow and extraction on sophora tonkinensis medicinal material by 70-95% ethyl alcohol to obtain fluid extract; conducting extraction with chloroform after watering suspension and acid adjusting treatment to obtain the total alkaloids; (2) conducting percolating extraction on the sophora tonkinensis medicinal material; conducting elution extraction by 732 cation exchange resin; conducting chloroform elution treatment on the resin to obtain the total alkaloids. The obtained total alkaloids are detected through a thin layer chromatography; trichloromethane-methyl alcohol-dense ammonia detection solution (4:1:0.1) is adopted as a developing solvent; sophocarpidine, oxymatrine and oxidized sophocarpine are taken as determined ingredients in the total alkaloids; the result is accurate and reliable. According to the invention, the extracting method is simple in process; the obtained total alkaloids are provided with pharmacologic action of gastric ulcer resistance, anticoagulation, cerebral ischemia resistance, antithrombus property, blood fat reduction, and the like, and are provided with relatively broad market development prospect.
Description
Technical field
The invention belongs to medicinal liquid extractive technique field, be specifically related to extracting method, detection method and the application thereof of total alkaloids in a kind of leafy Sophora tonkinensis Gagnep..
Background technology
Leafy Sophora tonkinensis Gagnep.
sophora tonkinensis gagnep.var. polyphylla s. Z.Huang et Z.C.Zhoufor cassia leguminous plant, with parts of generic medicinal plants Radix Sophorae Tonkinensis [formal name used at school: Sophora tonkinensis Gagnep.
sophora tonkinensisgagnep] be the equal root of the same race that belongs to together, be a mutation of Sophora tonkinensis Gagnep..Grow in Mountainous Area, be distributed in the provinces such as Guangxi, Guangdong, Yunnan, Guizhou and Jiangxi, at Guangxi main, will be distributed in basin, Hongsuihe River, as ground such as peace, Mashan, Xincheng, Wuming all.It is cold in nature, bitter in the mouth, have heat-clearing and toxic substances removing, detumescence sore-throat relieving effect, be usually used in fire-toxin and pent up, laryngopharynx swelling and pain, gingivitis, cough due to lung-heat, the treatment of the illness such as hepatitis.
Relevant leafy Sophora tonkinensis Gagnep. ingredient has had some researchs at present:
1, Lu Wenjie, tooth open health has been delivered " leafy Sophora tonkinensis Gagnep. liposoluble constituent research " article in < < Guangxi science > > fourth phase in 2011, adopt soxhlet extraction extract leafy Sophora tonkinensis Gagnep. (
sophora tonkinensisgagnep.var.
polyphyllas.Z.Huang et Z.C.Zhou) liposoluble constituent, after esterification, with gas chromatography-mass spectrography (GC-MS), analyze, computer search carries out component analysis and evaluation in conjunction with artificial parsing, be divided into from obtaining 41 peaks, identified wherein 32 kinds of compositions.Leafy Sophora tonkinensis Gagnep. liposoluble constituent is mainly containing long-chain fatty acid.All the components is all identified first in this plant.
2, Lu Wenjie, Lu Guoshou, Tan Xiao, Chen Jiayuan, yellow Zhou Feng has delivered the article of " research of leafy Sophora tonkinensis Gagnep. alkaloids composition " in < < Chinese experimental pharmacology of Chinese medical formulae magazine > > the 20th the 8th phase of volume in 2014, adopt Sephadex LH-20 column chromatography, silica gel column chromatography, the alkaloid component of the method separation such as recrystallization and the leafy Sophora tonkinensis Gagnep. of purification, integrated use infrared spectrum, mass spectrum, the technology such as nuclear magnetic resonance, NMR and physico-chemical method are analyzed, and the alkaloid structure that obtains of definite separation.Result: according to physicochemical property and spectral data, identified the structure of each compound respectively: sophocarpine, matrine, sophoranl, 9 Alpha-hydroxy matrines, 12 Alpha-hydroxy sophocarpine, N-oxysophocarpine, oxymatrine, eulexine.Conclusion: compound 1～8 is first separation from leafy Sophora tonkinensis Gagnep. and obtains.
Leafy Sophora tonkinensis Gagnep. has identical medicinal efficacy with Radix Sophorae Tonkinensis, Guangxi is among the people to be often used as leafy Sophora tonkinensis Gagnep. as Radix Sophorae Tonkinensis medical material and to use, < < Guangxi Chinese crude drug standard, 1990 editions > > also one of plant origin using leafy Sophora tonkinensis Gagnep. as Radix Sophorae Tonkinensis medical material record.According to one's analysis, leafy Sophora tonkinensis Gagnep. contain matrine the same as Radix Sophorae Tonkinensis, sophocarpine, sophoranl, 9 α – sophor-anols, 12 Alpha-hydroxy sophocarpine, oxymatrine, N-oxysophocarpine, the multiple alkaloid component such as eulexine.Radix Sophorae Tonkinensis faces day by day exhausted present situation in the market, therefore, total alkaloids in leafy Sophora tonkinensis Gagnep. is extracted to exploitation, can explore leafy Sophora tonkinensis Gagnep. medical material as the succedaneum of Radix Sophorae Tonkinensis, for the utilization of resources and clinical practice provide scientific basis, seem particularly important.
Summary of the invention
The object of the invention is the problem reducing gradually for existing market Radix Sophorae Tonkinensis resource, extracting method, detection method and the application thereof of total alkaloids in a kind of leafy Sophora tonkinensis Gagnep. are provided, using this explore leafy Sophora tonkinensis Gagnep. medical material can be as the succedaneum of Radix Sophorae Tonkinensis, for the utilization of resources and clinical practice provide scientific basis.
The object of the invention is to be achieved through the following technical solutions:
In the leafy Sophora tonkinensis Gagnep. of the present invention, the extracting method of total alkaloids is divided into two kinds:
First method is: leafy Sophora tonkinensis Gagnep. medical material is extracted and obtains fluid extract with 70-95% alcohol heating reflux, through adding after water suspendible and acid adjustment are processed, use chloroform extraction, finally obtain total alkaloids.
Concrete steps are: (1) becomes 20-80 order coarse powder by leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, by 70-95% ethanol reflux, extract, 3-5 time at 80-90 ℃, and each 1-3 hour, filtered while hot, recovery solvent, obtains the fluid extract that at 60-70 ℃, relative density is 1.10-1.20; (2) fluid extract is added to water suspendible, dripping concentrated sulphuric acid to pH value is 3; (3) with chloroform extraction, to chloroform color, shoal, it is 11 that sour water layer adds potassium carbonate to pH value, with chloroform extraction, to chloroform solution evaporate to dryness inanimate object alkali reaction, reclaims chloroform and obtains chloroform extract, obtains total alkaloids.
Second method is: first leafy Sophora tonkinensis Gagnep. medical material percolation is extracted, through 732 cation exchange resin eluting, extract, finally resin is carried out to the processing of chloroform eluting and obtain total alkaloids.
Concrete steps are: (1) becomes 20-80 order coarse powder by leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, with the 1% aqueous hydrochloric acid solution percolation of 8-20 times of leafy Sophora tonkinensis Gagnep. quality of medicinal material, extract 1-7 days, collect percolate; (2) percolate, by 732 cation exchange resin columns of anticipating, washes resin column with water colourless to water liquid; (3) resin is poured in unlimited vessel, dried, pinch moltenly with ammonia, by chloroform reflux, extract, 2-5 time, check inanimate object alkali reaction to chloroform extracted solution evaporate to dryness, recovery chloroform extracted solution obtains chloroform extract, obtains total alkaloids.
732 cation exchange resiies are strongly acidic styrene type cation exchange resin, it is a kind of sulfonated polystyrene gel-type strong-acid cation-exchange resin, have the advantages that exchange capacity is high, exchange velocity is fast, mechanical strength is high, comparatively suitable in extract total alkaloids process in leafy Sophora tonkinensis Gagnep., through inventor's multiple authentication, use this resin elution extract total alkaloids to there is higher yield.In addition, 732 cation exchange resin column preprocess methods are: resin is placed in to clean container, uses clear water rinsing, until draining is clear.Be soaked in water resin 12 ~ 24 hours, fully expands resin.As be dried resin, should first with saturated nacl aqueous solution, soak, more progressively dilute sodium chloride solution, broken in order to avoid resin sharply expands suddenly.With 2 ~ 5%HCl solution soaking resin of 2 times of amounts of resin volume 2 ~ 4 hours, and frequently stir.Then use low pure water washing resin, until pH value of solution is close to 4, then uses 2 ~ 5%NaOH solution-treated, after processing, wash with water to neutrality.
For better controlling the quality of total alkaloids in leafy Sophora tonkinensis Gagnep., inventor also repeatedly explores and studies the total alkaloids extracting from leafy Sophora tonkinensis Gagnep., has formulated its examination criteria, and concrete detection method is:
The total alkaloids by thin layer chromatography qualitative identification, take the multi leaf total alkaloids of Sophora tonkinensis gapnep 10mg, placed in a 10ml volumetric flask, add chloroform to scale, shake up, as the test solution; take leafy Sophora tonkinensis gapnep medicinal powder 0.5g, add chloroform 10ml, strong ammonia solution 0.2ml, shake for 15 minutes, filtration, the filtrate evaporate the dry residue 0.5ml, add chloroform dissolved, as the control medicine solution; another matrine and Oxymatrine control products, control products, Oxysophocarpine control and chloroform mixed solution made of every 1ml each containing 1mg, as the reference solution, thin-layer chromatography test, draw the test solution, control the medicinal solution of 1~2? L, the reference solution of 4~6? L, respectively, point to the same silica gel G thin layer plate, with chloroform methanol concentrated ammonia solution (4:1:0.1) as the agent, expansion, removed, dried, bismuth potassium iodide solution to dilute spray, in the chromatography of samples, with the control herbs, control position chromatograph corresponding, show the same orange spots.
Total alkaloids in the leafy Sophora tonkinensis Gagnep. of the present invention, is preparing anti-gastric-ulcer, anticoagulation, anti-cerebral ischemia, the application in antithrombotic and blood lipid-lowering medicine.Through inventor, verify repeatedly, leafy Sophora tonkinensis Gagnep. total alkaloids high dose and total alkaloids of subprostrate sophora root high dose all can alleviate mouse gastric ulcer index, extend clotting time of mice, extend mouse brain ischemia breathing time, reduce rat vein thrombus weight, reduce hyperlipemia in mice blood lipid level, both act on quite; In leafy Sophora tonkinensis Gagnep. total alkaloids, dosage can alleviate mouse gastric ulcer index, extends mouse brain ischemia breathing time, reduces rat vein thrombus weight; Acute toxicity result shows, the median lethal dose(LD 50) (LD of leafy Sophora tonkinensis Gagnep. total alkaloids
50) be 55.30g.kg
-1, the 95% credible 48.15～64.37g.kg that is limited to
-1.
In leafy Sophora tonkinensis Gagnep. of the present invention, extracting method, detection method and the application thereof of total alkaloids have following marked improvement and advantage:
One, the extracting method of total alkaloids in leafy Sophora tonkinensis Gagnep. of the present invention, can use alcohol reflux to obtain fluid extract, then carry out the method for chloroform extraction, after can also using aqueous hydrochloric acid solution percolation to extract, through 732 cation exchange resin column eluting, the last method by chloroform extraction, above two kinds of extracting method are all without high temperature, environment under high pressure, leaching process is simple, easy to operate, easy to control, more easily realize suitability for industrialized production, for the batch extracting of total alkaloids in leafy Sophora tonkinensis Gagnep., provide full and accurate, reliable data information.
Two, the detection method of total alkaloids in leafy Sophora tonkinensis Gagnep. of the present invention, by matrine in the qualitative total alkaloids of thin layer chromatography, oxymatrine and N-oxysophocarpine are controlled the quality of total alkaloids, comparatively accurate.Because matrine, oxymatrine and N-oxysophocarpine are main components wherein in total alkaloids, in its application, play Main Function.Through inventor's multiple authentication, take chloroform-methanol-strong ammonia solution (4:1:0.1) as developing solvent for the main component in qualitative total alkaloids accurately, reliable, use thin layer chromatography flow process simple simultaneously, repeatability is high, is suitable as the production On-line Control of total alkaloids in leafy Sophora tonkinensis Gagnep. and the control criterion method of dispatching from the factory.
Three, the total alkaloid content that in leafy Sophora tonkinensis Gagnep. of the present invention, the extracting method of total alkaloids extracts is higher, through inventor, its pharmaceutical research is found, leafy Sophora tonkinensis Gagnep. total alkaloids high dose and total alkaloids of subprostrate sophora root high dose all can alleviate mouse gastric ulcer index, extend clotting time of mice, extend mouse brain ischemia breathing time, reduce rat vein thrombus weight, reduce hyperlipemia in mice blood lipid level, both act on quite, leafy Sophora tonkinensis Gagnep. total alkaloids has certain anti-gastric-ulcer, anticoagulation, anti-cerebral ischemia, antithrombotic and effect for reducing blood fat, its toxicity is lower.
the specific embodiment
total alkaloids extracting method in the leafy Sophora tonkinensis Gagnep. of embodiment 1 (method one)
1-1 becomes 20 order coarse powder by leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, and by 70% ethanol reflux, extract, 5 times at 90 ℃, each 1 hour, filtered while hot, reclaimed solvent, obtains the fluid extract that at 60 ℃, relative density is 1.20; Fluid extract is added to water suspendible, and dripping concentrated sulphuric acid to pH value is 3; With chloroform extraction, to chloroform color, shoal, it is 11 that sour water layer adds potassium carbonate to pH value, with chloroform extraction, to chloroform solution evaporate to dryness inanimate object alkali reaction, reclaims chloroform and obtains chloroform extract, obtains total alkaloids.
1-2 becomes 80 order coarse powder by leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, and by 75-90% ethanol reflux, extract, 4 times at 85 ℃, each 2 hours, filtered while hot, reclaimed solvent, the fluid extract that while obtaining 65 ℃, relative density is 1.15; Fluid extract is added to water suspendible, and dripping concentrated sulphuric acid to pH value is 3; With chloroform extraction, to chloroform color, shoal, it is 11 that sour water layer adds potassium carbonate to pH value, with chloroform extraction, to chloroform solution evaporate to dryness inanimate object alkali reaction, reclaims chloroform and obtains chloroform extract, obtains total alkaloids.
1-3 becomes 50 order coarse powder by leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, and by 95% ethanol reflux, extract, 3 times at 80 ℃, each 3 hours, filtered while hot, reclaimed solvent, the fluid extract that while obtaining 70 ℃, relative density is 1.10; Fluid extract is added to water suspendible, and dripping concentrated sulphuric acid to pH value is 3; With chloroform extraction, to chloroform color, shoal, it is 11 that sour water layer adds potassium carbonate to pH value, with chloroform extraction, to chloroform solution evaporate to dryness inanimate object alkali reaction, reclaims chloroform and obtains chloroform extract, obtains total alkaloids.
total alkaloids extracting method (method two) in the leafy Sophora tonkinensis Gagnep. of embodiment 2
2-1 becomes coarse powder by 20 kilograms of leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, crosses 20 mesh sieves, puts into percolator, and then compacting layer by layer puts into percolator with 160 kilograms of 1% aqueous hydrochloric acid solutions preparing, airtight, extracts 7 days, in percolation process, does not stir, and collects percolate; By 732 cation exchange resin columns of anticipating, then wash percolate with water resin column colourless to water liquid; Resin is poured in unlimited container, dried or dry at 55-65 ℃, with ammonia, pinch moltenly, by chloroform reflux, extract, 5 times, check inanimate object alkali reaction to chloroform extracted solution evaporate to dryness, reclaim chloroform extracted solution and obtain chloroform extract, obtain total alkali.
2-2 becomes coarse powder by 15 kilograms of leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, crosses 20 mesh sieves, puts into percolator, and then compacting layer by layer puts into percolator with 300 kilograms of 1% aqueous hydrochloric acid solutions preparing, airtight, extracts 1 day, in percolation process, does not stir, and collects percolate; By 732 cation exchange resin columns of anticipating, then wash percolate with water resin column colourless to water liquid; Resin is poured in unlimited container, dried or dry at 55-65 ℃, with ammonia, pinch moltenly, by chloroform reflux, extract, 2 times, check inanimate object alkali reaction to chloroform extracted solution evaporate to dryness, reclaim chloroform extracted solution and obtain chloroform extract, obtain total alkali.
2-3 becomes coarse powder by 30 kilograms of leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials, crosses 20 mesh sieves, puts into percolator, and then compacting layer by layer puts into percolator with 450 kilograms of 1% aqueous hydrochloric acid solutions preparing, airtight, extracts 3-5 days, in percolation process, does not stir, and collects percolate; By 732 cation exchange resin columns of anticipating, then wash percolate with water resin column colourless to water liquid; Resin is poured in unlimited container, dried or dry at 55-65 ℃, pinch moltenly with ammonia, by chloroform reflux, extract, 3-4 time, check inanimate object alkali reaction to chloroform extracted solution evaporate to dryness, recovery chloroform extracted solution obtains chloroform extract, obtains total alkali.
In said extracted method, 732 cation exchange resin column preprocess methods are: resin is placed in to clean container, uses clear water rinsing, until draining is clear.Be soaked in water resin 15 hours, fully expands resin.With the 5%HCl solution soaking resin of 2 times of amounts of resin volume 4 hours, and frequently stir, then use low pure water washing resin, until pH value of solution is close to 4, then uses 5%NaOH solution-treated, after processing, wash with water to neutrality.
732 cation exchange resin specifications and models are as follows:
the detection method of total alkaloids in the leafy Sophora tonkinensis Gagnep. of embodiment 3
Take the embodiment of income of 1 or 2 extraction methods of total alkaloids of Sophora 10mg multi leaf Vietnam, placed in a 10ml volumetric flask, add chloroform to scale, shake up, as the test solution; take leafy Sophora tonkinensis gapnep medicinal powder 0.5g, add chloroform 10ml, strong ammonia solution 0.2ml, shake for 15 minutes, filtration, the filtrate evaporated, the residue with 0.5ml chloroform dissolved, as the control medicine solution; another matrine and Oxymatrine control products, control products, Oxysophocarpine control and chloroform mixed solution made of every 1ml each containing 1mg, as the reference solution, thin-layer chromatography test, draw the test solution, the control medicine solution 1~2? L, the reference solution of 4~6? L, respectively, point to the same silica gel G thin layer plate, with chloroform methanol concentrated ammonia solution (4:1:0.1) as the agent, expansion, removed, dried, bismuth potassium iodide solution to dilute spray, in the chromatography of samples, in the control of medicinal herbs, the control position chromatograph corresponding, show the same orange spots.
embodiment 4 effect embodiment
Inventor has carried out many pharmacological research to the total alkaloids extracting from leafy Sophora tonkinensis Gagnep., and medicine on probation is as follows:
Leafy Sophora tonkinensis Gagnep. total alkaloids (in leafy Sophora tonkinensis Gagnep. from carry-embodiment 1 or 2);
Total alkaloids of subprostrate sophora root ointment; Guangxi traditional Chinese medicine academy chemistry provides;
Cimetidine slice, Jiangxi Jimin Kexin Drug Industry Co., Ltd;
Aspirin Enteric-coated Tablets, Wuhan Yuanda Pharmaceutical Group Co., Ltd.'s product;
FUFANG DANSHEN PIAN, White Cloud Mountain, Guangdong Chinese medicine company limited of Hutchison China Trade Holdings product;
XUEZHIKANG JIAONANG, Beijing WBL Peking University Biotech Co., Ltd's product.
For trying animal: Kunming mouse, body weight 18-22g, male and female half and half; SD rat, 200 ± 20g, male and female half and half, are provided credit number by Guangxi Medical University's Experimental Animal Center: SCXK osmanthus 2009-0002, SPF level.Rat feeding in air conditioning labor, 22 ± 2 ℃ of room temperatures, 60 ± 5 ℃ of humidity, freely drink water and ingest.
Inventor has carried out the impact of anti-gastric-ulcer, anticoagulation, cerebral hypoxia ischemia, anti-phlebothrombosis and lipidemia on mice, concrete outcome is in Table 1-table 5.
The present invention carries out the pharmacological research such as antiulcer, anticoagulation, anti-cerebral ischemia, antithrombotic, blood fat reducing to leafy Sophora tonkinensis Gagnep. total alkaloids and total alkaloids of subprostrate sophora root under the parallel condition of preparation, dosage, the route of administration of medicine.Experimental result shows, leafy Sophora tonkinensis Gagnep. total alkaloids and total alkaloids of subprostrate sophora root all can alleviate mouse gastric ulcer index, extend clotting time of mice, extend mouse brain ischemia breathing time, reduce rat vein thrombus weight and reduce hyperlipemia in mice blood lipid level; Leafy Sophora tonkinensis Gagnep. total alkaloids 200mg.kg
-1with total alkaloids of subprostrate sophora root 200mg.kg
-1the effect degree of pharmacodynamics index is suitable.Confirm that leafy Sophora tonkinensis Gagnep. total alkaloids and total alkaloids of subprostrate sophora root have close pharmacological action, achievement in research of the present invention substitutes Radix Sophorae Tonkinensis production total alkaloids with leafy Sophora tonkinensis Gagnep. becomes possibility, in future, will have imponderable market prospect and value.
Claims (6)
1. an extracting method for total alkaloids in leafy Sophora tonkinensis Gagnep., is characterized in that: comprise the following steps:
(1) leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials is become to 20-80 order coarse powder, by 70-95% ethanol reflux, extract, 3-5 time at 80-90 ℃, each 1-3 hour, filtered while hot, recovery solvent, obtains the fluid extract that at 60-70 ℃, relative density is 1.10-1.20;
(2) fluid extract is added to water suspendible, dripping concentrated sulphuric acid to pH value is 3;
(3) with chloroform extraction, to chloroform color, shoal, it is 11 that sour water layer adds potassium carbonate to pH value, with chloroform extraction, to chloroform solution evaporate to dryness inanimate object alkali reaction, reclaims chloroform and obtains chloroform extract, obtains total alkaloids.
2. an extracting method for total alkaloids in leafy Sophora tonkinensis Gagnep., is characterized in that, comprises the following steps:
(1) leafy Sophora tonkinensis Gagnep. pulverizing medicinal materials is become to 20-80 order coarse powder, with 1% aqueous hydrochloric acid solution percolation, extract 1-7 days, collect percolate;
(2) percolate, by 732 cation exchange resin columns of anticipating, washes resin column with water colourless to water liquid;
(3) resin is poured in unlimited vessel, dried, with ammonia, pinch moltenly, use chloroform reflux, extract,, check inanimate object alkali reaction to chloroform extracted solution evaporate to dryness, reclaim chloroform extracted solution and obtain chloroform extract, obtain total alkaloids.
3. the extracting method of total alkaloids in leafy Sophora tonkinensis Gagnep. according to claim 2, is characterized in that: described percolation in extracting, add 1% aqueous hydrochloric acid solution be leafy Sophora tonkinensis Gagnep. quality of medicinal material 8-20 doubly.
4. the extracting method of total alkaloids in leafy Sophora tonkinensis Gagnep. according to claim 2, is characterized in that: described in step (3), chloroform reflux, extract, is 2-5 time.
5. the detection method of the leafy Sophora tonkinensis Gagnep. total alkaloids that the extracting method described in claim 1-2 any one obtains, is characterized in that: adopt thin layer chromatography to carry out qualitative identification, concrete steps are:
(1) get leafy Sophora tonkinensis Gagnep. total alkaloids 10mg, put in 10ml volumetric flask, add chloroform to scale, shake up, as need testing solution;
(2) get leafy Sophora tonkinensis Gagnep. medicinal powder 0.5g, add chloroform 10ml, strong ammonia solution 0.2ml, jolting 15 minutes, filters, filtrate evaporate to dryness, residue adds 0.5ml chloroform to be made to dissolve, in contrast medical material solution;
(3) separately get matrine reference substance, oxymatrine reference substance, N-oxysophocarpine reference substance adds chloroform and makes every 1ml respectively containing the mixed solution of 1mg, in contrast product solution;
(4) and extracting the test solution, the control medicine solution each 1~2? L, the reference solution of 4~6? L, respectively, point to the same silica gel G thin layer plate, with chloroform methanol concentrated ammonia solution (4:1:0.1) as the agent, expansion, removed, dried, by spraying dilute bismuth potassium iodide test solution, in the chromatography of samples, in with contrast medicinal material, control position chromatograph corresponding, show the same orange spots.
6. the leafy Sophora tonkinensis Gagnep. total alkaloids that the extracting method described in claim 1-2 any one obtains, is preparing anti-gastric-ulcer, anticoagulation, anti-cerebral ischemia, the application in antithrombotic and blood lipid-lowering medicine.
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| CN114903930A (en) * | 2022-06-17 | 2022-08-16 | 诺斯贝尔化妆品股份有限公司 | Preparation and application of anti-inflammatory and soothing sophora flavescens extract and matrine compound |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106588929A (en) * | 2016-12-31 | 2017-04-26 | 南宁馨艺荣生物科技有限公司 | Method for extracting and purifying oxysophocarpine from radix Sophorae Tonkinensis |
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| CN109856287A (en) * | 2019-03-25 | 2019-06-07 | 广西壮族自治区药用植物园 | The detection method of alkaloid in toothpaste |
| CN114903930A (en) * | 2022-06-17 | 2022-08-16 | 诺斯贝尔化妆品股份有限公司 | Preparation and application of anti-inflammatory and soothing sophora flavescens extract and matrine compound |
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Application publication date: 20141015 Assignee: Guangxi Houde Pharmaceutical Co.,Ltd. Assignor: GUANGXI INSTITUTE OF CHINESE MEDICINE & PHARMACEUTICAL SCIENCE Contract record no.: X2023980045210 Denomination of invention: Extraction, detection, and application of total alkaloids from multi leaf Vietnamese locust Granted publication date: 20161130 License type: Common License Record date: 20231102 |
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