CN104094895A - Method for cleansing epidemic diseases on scale pig farm - Google Patents
Method for cleansing epidemic diseases on scale pig farm Download PDFInfo
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- CN104094895A CN104094895A CN201410358986.XA CN201410358986A CN104094895A CN 104094895 A CN104094895 A CN 104094895A CN 201410358986 A CN201410358986 A CN 201410358986A CN 104094895 A CN104094895 A CN 104094895A
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Abstract
The invention relates to the technical field of epidemic disease immunization on pig farms, in particular to a method for cleansing epidemic diseases on a scale pig farm. The method comprises the following steps: sampling serum samples of boars in phases and sampling serum samples of piglets according age in days; detecting an immune antibody and an infection antibody; revising an immune process according to results. By adopting the method, the problems involved in prevention and control, particularly immediate cleansing of major epidemic diseases on the scale pig farm are solved, the morbidity and mortality of pig diseases on the scale pig farm are controlled, the immune effect is improved, and the economic benefit is increased.
Description
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Technical field
The present invention relates to epidemic disease immunological technique field, pig farm, concrete a kind of scale pig farm purifies the method for epidemic disease.
Background technology
Along with the development of pig industry, scale and intensive level improve constantly, the mobile increasing of live pig and products thereof simultaneously, the thing followed is exactly that the M & M of various swine diseases progressively increases, especially Schweineseuche, swine fever has been the sick kind of the compulsory immunization of China's legal provisions, porcine pseudorabies, the swine diseases such as blue otopathy also become the main epidemic disease of harm pig industry, China's these swine diseases of prevention and control are mainly taked the method for vaccine injection at present, i.e. the be correlated with vaccine injection of epidemic disease of special time after piglet birth, after injection certain hour, body produces specific antibody, resist the infection of wild poison, thereby reach the object of prevention epidemic disease.What China taked is mainly Schweineseuche, swine fever spring, concentrate immunity autumn, and other epidemic diseases are the policy of immunity voluntarily, is sequencing immunity and scale hog man will take.
Immune antiboidy is not forever to exist in body; it is along with the continuous invasion and attack of wild poison or the continuity meeting of time reduce gradually; the invasion and attack that finally can not resist wild poison cause animal morbidity; make antibody return to guard mode so must carry out in time booster immunization in the time that antibody declines; make immune antiboidy in animal body keep for a long time certain level, just can play to antilemic effect.
The unique channel of inspection immune effect is exactly that immune antiboidy detects, and conventional is that immunity gathers serum sample for latter 21 days at present, carries out immune antiboidy detection through hemagglutination test or Elisa test.Its major defect and deficiency are that can only detect sampling time, body immune antiboidy exists situation, and can not distinguish immune antiboidy and infect antibody (antibody that zoogenetic infection epidemic disease produces), and there is no continuity, only has the distribution situation of Fluctuation and the wild virus infection antibody of having grasped immune antiboidy, could carry out vaccine immunity injection in good time, make immune antiboidy level in body remain on for a long time higher level.
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Summary of the invention
Object of the present invention is exactly to have situation in order to overcome body immune antiboidy only can detect sampling in prior art time, and can not distinguish immune antiboidy and infect antibody, and there is no successional defect, the method of a kind of scale pig farm purification epidemic disease is provided, has purified especially in time the problem of scale pig farm epidemic disease to solve prevention and control.
In order to solve the problems of the technologies described above, the present invention adopts following technical proposals:
Scale pig farm purifies a method for epidemic disease, comprises the steps:
(1) boar stage by stage, piglet press age in days gather serum sample;
(2) immune antiboidy and infection antibody test;
(3) according to epidemic disease immune antiboidy Fluctuation with infect antibody distribution situation, adjust relevant epidemic disease vaccine immunity inject time, immunization method is adjusted.
As preferably, described scale pig farm is in pig farm, to have 100 of basic sow livestock on hands, stock boar to have more than 5.
As preferably, the boar described in step (1) is stock boar, replacement gilt, 1-2 tire sow, 3-4
Tire sow, 5-6 tire sow or the above sow of 6 tires.
As preferably, the piglet described in step (1) is the piglet in 1 week postpartum, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks, 14 weeks.
As preferably, boar described in step (1) stage by stage, to gather serum sample by age in days be that stock boar is all sampled to piglet; Replacement gilt, 1-2 tire sow, 3-4 tire sow, 5-6 tire sow, the above sow of 6 tire, each raising stage is the conventional serum sample that gathers respectively, and each stage hits is no less than 5 parts; At the piglet conventional serum sample that gathers of 1 week postpartum, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks, 14 weeks, each stage hits is no less than 5 parts respectively.
As preferably, described in step (1), serum sample is limpid without haemolysis, at least 1ml.
As preferably, described in step (1), sample without carrying out same pig, can be in the same period not
Complete sample collection with in raising stage pig.
As preferably, described in step (2), antibody detection method is Elisa test method.
As preferably, the described adjustment immunization method of step (3) is to adjust relevant epidemic disease vaccine immunity inject time.
Compared with prior art, the beneficial effect that the present invention brings is as follows:
(1) can grasp in time in scale pig farm immune effect and the wild virus infection situation of relevant epidemic disease;
(2) adjust immunization method in good time, reduce the M & M of swine disease, increase economic benefit;
(3) adhere to carrying out every year at least 2 assessments simultaneously, carry out continuously 2-3, be aided with strict feeding and management, can reach the object of scale pig farm cleanse of epidemic disease.
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Brief description of the drawings
?fig. 1 is the different raising stage hog cholera antibody Fluctuation figure in scale of the present invention pig farm;
Fig. 2 is scale of the present invention pig farm pathogeny of hog cholera distribution map.
Embodiment
Below in conjunction with embodiment, the present invention is further elaborated, but these embodiment do not form any restriction to the present invention.
Carry out hog cholera immune method taking scale pig farm as example explanation embodiments of the present invention.
1, pig farm situation
600 of existence foundation sows, 10 of stock boars, year is delivered 8000 of market pigs for sale, and former immunization method is: piglet 28 age in days head exempt from newborn rabbit seedling, and 50 ages in days 2 are exempted from spleen and are drenched seedling, and 110 age in days spleens drench 1 part of seedlings; Before replacement gilt breeding, spleen drenches 2 parts of seedling; Sow spleen in 28 days postpartum drenches 2 parts of seedling; Annual 2 spleens of boar drench 2 parts of seedling.
2, sample collection
By stock boar, replacement gilt, 1-2 tire sow, 3-4 tire sow, 5-6 tire sow, piglet 2,4,6,8,10,12,14 weeks, except stock boar is all sampled, each stage gathers respectively 10 part serum samples.
3, test operation
Swine fever antigen Elisa detection kit, hog cholera antibody blocking-up Elisa kit, purchased from century Yuan Heng biotech firm (Hai Bolai and Switzerland PRIONICG product).Undertaken by reagent specification, strictly establish yin and yang attribute contrast.Hog cholera antibody value >=40 are that immunity is qualified, and swine fever antigen PP >=15% is positive.
4, interpretation of result
4.1 hog cholera immune Antibodies
Calculate respectively immune qualification rate, mean value and dispersion by sampled packet, by sequentially built immune antiboidy its growth figure in age in week, front 2 indexs of above 3 indexs are higher than critical value, and dispersion is that immune state is good lower than 40%, otherwise immune state is undesirable; Except after inoculation about 1 month, coordinate more than 20%, be traced reason before and after being higher or lower than appears in immune antiboidy mean value.Concrete condition is in shown in table 1, Fig. 1.
Table 1: certain scale pig farm swine fever antibody test result
| Age in days | 2W | 4W | 6W | 8W | 12W | 14W | 16W | 18W | P1-2 | P3-4 | P5-6 | P＞6- | Boar | Standby |
| Mean value | 68 | 35.5 | 33 | 44.5 | 57.5 | 47.6 | 38.4 | 47.5 | 48.6 | 58.4 | 47.9 | 53.2 | 55 | 51.3 |
| Sample number | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 20 | 10 |
| Passing number | 9 | 4 | 4 | 8 | 9 | 8 | 5 | 8 | 9 | 8 | 10 | 10 | 18 | 8 |
| Qualification rate % | 90 | 40 | 40 | 80 | 90 | 80 | 50 | 80 | 90 | 80 | 100 | 100 | 90 | 80 |
| Dispersion CV% | 7.07 | 53.14 | 50.33 | 32.70 | 28.40 | 23.67 | 41.50 | 27.42 | 7.47 | 10.14 | 23.27 | 14.54 | 23.11 | 12.86 |
As can be seen from the above results, this pig farm hog cholera immune is except 4W, 6W, 16W week, and other stage immune effects are good, and colony's Antibody qualification rate is all more than 70%.
4.2 pathogeny of hog cholera interpretations of result
For preventing pathogeny of hog cholera diffusion, early stage cause of disease detects extremely important, within 1 week-3 weeks after infection, just there will be because swine fever infects antibody.Calculate respectively infection rate by sampled packet, to occurring that positive group (or each group all has the positive, certain group positive rate outstanding) will trace reason, and orientate the emphasis that immune programme for children is adjusted as.In table 2 and Fig. 2.
Table 2: certain scale pig farm pathogeny of hog cholera testing result
| Age in days | 2W | 4W | 6W | 8W | 12W | 14W | 16W | 18W | P1-2 | P3-4 | P5-6 | P＞6- | Boar | Standby |
| Mean value % | 0 | 6.21 | 5.95 | 1.65 | 0 | 0 | 18.4 | 0 | 3.2 | 5.32 | 17.9 | 13.2 | 15.23 | 0 |
| Sample number | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 20 | 10 |
| Number positive | 0 | 2 | 3 | 1 | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 4 | 6 | 0 |
| Positive rate % | 0 | 20 | 30 | 10 | 0 | 0 | 30 | 0 | 10 | 10 | 10 | 40 | 30 | 0 |
This pig farm is before 2 week age, 12,14,18 week age and replacement gilt infect without pathogeny of hog cholera as can be seen from the above results, other each stages have pathogeny of hog cholera in various degree to infect, within wherein 6 weeks, 16 weeks, sow infection rates more than 6 tires is the highest, should change relevant prevention and control measure.
5, prevention and control measure
5.1 immune programme for children amendments
According to antibody and cause of disease testing result, in view of this 4W, 6W, 16W hog cholera immune antibody horizontal are lower, and pathogen infection is heavier, the Immuno-programme for Hog Cholera is revised as to 21 days head exempt from, two exempt from constant, at 105 days booster immunizations once.
5.2 measure of sifting out
Serious in view of high parity sow pathogen infection, advise this superseded high parity sow, eliminate the positive stock boars of all cause of diseases simultaneously.Eliminate the multiparity sow of the doubtful swine fever symptoms such as 1-6 tire clinical manifestation miscarriage, product stillborn foetus (weak tire).
5.3 strengthen feeding and management, establish and improve environment control measure.In purification process, strengthen feeding and management and assanation control, especially must strengthen management in the mobile aspect such as introduced pigs and commodities for export sales pig, personnel, feed, prevent from purifying swinery subinfection again, forbid raising other animals such as dog, cat, poultry simultaneously, and will carry out the measure such as protection against rodents, anti-wild bird.
6, clean-up effect
By adopting the present invention to purify the method for scale pig farm pestilence, adhere to carrying out for every 6 months once assessment, and adjust immunization method according to assessment result in good time, in March, 2014, this pathogeny of hog cholera Elisa testing result total negative, reaches the clean-up effect of expection.
The explanation of above embodiment is just for helping to understand method of the present invention and core concept thereof.It should be pointed out that for a person skilled in the art, under the premise of not departing from the present invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of the claims in the present invention.
Claims (9)
1. scale pig farm purifies a method for epidemic disease, it is characterized in that, comprises the steps:
(1) boar stage by stage, piglet press age in days gather serum sample;
(2) immune antiboidy and infection antibody test;
(3) according to epidemic disease immune antiboidy Fluctuation and infection antibody distribution situation, adjust immunization method.
2. the method that purifies epidemic disease according to the scale pig farm described in claim 1, is characterized in that, described scale pig farm is in pig farm, to have 100 of basic sow livestock on hands, stock boar to have more than 5.
3. the method that purifies epidemic disease according to the scale pig farm described in claim 1 or 2, is characterized in that, the boar described in step (1) is stock boar, replacement gilt, 1-2 tire sow, 3-4 tire sow, 5-6 tire sow or the above sow of 6 tires.
4. the method that purifies epidemic disease according to the scale pig farm described in claim 3, is characterized in that, the piglet described in step (1) is the piglet in 1 week postpartum, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks, 14 weeks.
5. the method that purifies epidemic disease according to scale pig farm described in claim 3, is characterized in that, boar described in step (1) stage by stage, to gather serum sample by age in days be that stock boar is all sampled to piglet; Replacement gilt, 1-2 tire sow, 3-4 tire sow, 5-6 tire sow, the above sow of 6 tire, each raising stage is the conventional serum sample that gathers respectively, and each stage hits is no less than 5 parts; At the piglet conventional serum sample that gathers of 1 week postpartum, 2 weeks, 4 weeks, 6 weeks, 8 weeks, 10 weeks, 12 weeks, 14 weeks, each stage hits is no less than 5 parts respectively.
6. scale according to claim 5 pig farm purifies the method for epidemic disease, it is characterized in that,
Described in step (1), serum sample is limpid without haemolysis, at least 1ml.
7. the method that purifies epidemic disease according to the scale pig farm described in claim 6, is characterized in that, sampling described in step (1), without carrying out same pig, can in the same period complete sample collection in pig of different raising stages.
8. the method that purifies epidemic disease according to the scale pig farm described in claim 1 or 2, is characterized in that, described in step (2), antibody detection method is Elisa test method.
9. the method that purifies epidemic disease according to the scale pig farm described in claim 1, is characterized in that, the described adjustment immunization method of step (3) is the relevant epidemic disease vaccine immunity of adjustment inject time.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105010235A (en) * | 2015-08-14 | 2015-11-04 | 湖南新南方养殖服务有限公司 | Method for cleaning pig farm to prevent diseases |
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| CN103385211A (en) * | 2013-07-12 | 2013-11-13 | 郭传城 | Boar healthy breeding method |
| CN103621470A (en) * | 2013-12-12 | 2014-03-12 | 李庆海 | Disease-free cultivation technology, namely comprehensive swine disease prevention and treatment measure |
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| CN101253853A (en) * | 2008-04-10 | 2008-09-03 | 华中农业大学 | Breeding method cleaning pig disease 60system small unit |
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Application publication date: 20141015 |