CN104094865A - Rotary tracking system capable of enabling rats to have stress response - Google Patents
Rotary tracking system capable of enabling rats to have stress response Download PDFInfo
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- CN104094865A CN104094865A CN201410202407.2A CN201410202407A CN104094865A CN 104094865 A CN104094865 A CN 104094865A CN 201410202407 A CN201410202407 A CN 201410202407A CN 104094865 A CN104094865 A CN 104094865A
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Abstract
The invention discloses a rotary tracking system capable of enabling rats to have stress response. The rotary tracking system comprises a bracket (1); a motor (2) and a motor control device (3) are fixedly arranged on the bracket (1); an output shaft (4) of the motor (2) is connected with a rotary platform (6) by a bearing (5), and the rotary platform (6) is horizontally arranged; a plurality of rat cages (7) are fixedly arranged on the rotary platform (6); each rat cage (7) is divided into an upper layer and a lower layer by grids (8); a metabolism collection box (9) is arranged at the lower layer of each rat cage. A plurality of rats can be respectively arranged in the rat cages, and the rat cages can rotate along the horizontal direction, so that the problem that the rats are injured due to extruding or falling off from the vertexes of the cages can be avoided; the motor control device is used for controlling the rotating speed of the motor, so that metering is conveniently carried out, the repeatability and the consistency of experiments can be guaranteed, and a psychological stress animal model can be built; during the experiment, the excrement of the rats can be collected for the experimental analysis.
Description
Technical field
The present invention relates to a kind of medical research experimental provision, especially a kind of rotation tracking system that causes mouse generation stress reaction that builds Animal Model for Psychological Stress.
Background technology
Psychological stress can improve the probability that Subjects with Impair Glucose Tolerant develops into diabetes, and its mechanism is mainly by affecting the mood loop of hypothalamus, limbic system, causes insulin sensitivity to reduce, and brings out insulin resistance, promotes the generation of diabetes.Although existing bibliographical information at present, rotatory stimulation can simulating human because diabetogenic pathogenic process is led in psychological stress, do not have Special experimental equipment, be not all that many rats are placed in same cage conventionally, by the cage that manually rolls, stimulated.There are the following problems:
1. in rolling process, rat can be injured owing to pushing or throwing from cage summit;
2. be difficult to the number of turns that metering is rolled, cause repeatability and the uniformity of experiment poor, be difficult to build Animal Model for Psychological Stress;
3. can not collect the excreta of rat, in time experiment Analysis.
Summary of the invention
The present invention is in order to solve the existing above-mentioned technical problem of prior art, and a kind of rotation tracking system that causes mouse generation stress reaction that builds Animal Model for Psychological Stress is provided.
Technical solution of the present invention is: a kind of rotation tracking system that causes mouse generation stress reaction, be provided with support, on support, be fixed with motor and motor control assembly, the output shaft of motor joins by bearing and horizontally disposed rotation platform, on rotation platform, be fixed with a plurality of mouse cages, described mouse cage is divided into two-layer up and down by grid, under be provided with metabolism disposable box.
Described a plurality of mouse cage is uniform along rotation platform circumferencial direction.
The present invention can be placed in a plurality of rats respectively in a plurality of mouse cages and choosing in the horizontal direction turns, and avoids rat because of extruding or throws injured problem from cage summit; By motor control assembly, control motor speed, convenient metering, has guaranteed repeatability and the uniformity of testing, and can build Animal Model for Psychological Stress; The excreta that can collect rat when testing is for experimental analysis.
Accompanying drawing explanation
Fig. 1 is the overall structure schematic diagram of the embodiment of the present invention 1,2.
Fig. 2 is the cage-shaped structure schematic diagram of the embodiment of the present invention 1,2.
Fig. 3 is the vertical view of the embodiment of the present invention 1.
Fig. 4 is the vertical view of the embodiment of the present invention 2.
Embodiment
Embodiment 1:
As shown in Figure 1, 2, 3: have support 1, on support 1, be fixed with motor 2 and motor control assembly 3, the output shaft 4 of motor 2 is by connector connection rotating shaft, by bearing 5 and horizontally disposed rotation platform 6, join again, in rotation platform 6 footpaths, upwards by connector (bolt, slot etc.), be fixed with a plurality of mouse cages 7, for convenience detach.Size 25cm * 10cm * the 13cm of described mouse cage 7, by grid 8, be divided into two-layer up and down, upper and lower two-layer be equipped with can switch door, be convenient to place rat Ji lower floor metabolism disposable box 9 be set.
Embodiment 2:
As shown in Fig. 1,2,4: structure is substantially the same manner as Example 1, with embodiment 1 difference be that a plurality of mouse cages 7 are uniform along rotation platform 6 circumferencial directions.Uniform along rotation platform 6 circumferencial directions, can guarantee, under the condition of same angular speed and linear velocity, rat to be rotated to experiment, further guaranteed repeatability and the uniformity of experiment.
Experimentation:
Open motor control switch, by motor control assembly 3, regulate motor 2 rotating speeds, motor 2 output shaft 4 driven rotary platforms 6 rotate, and 6 of rotation platforms are with mouse cage 7 synchronous rotaries.In rotary course, the excreta of rat is collected by metabolism disposable box 9.After rotation finishes, metabolism disposable box 9 can be taken out, collected excreta, for experimental analysis, can continue after metabolism disposable box 9 is rinsed well to use.
Adopt the embodiment of the present invention 2 to carry out rotatory stimulation experiment.Revolution 33rpm/min, time 15min/ time, to rotate every day 4 times, the next day, carries out once, totally 6 weeks.
Experimental result is as follows:
5.1 animal groupings: blank group (cont), psychological stress group (PS)
5.2 respectively organize the cortisol levels comparison of rat blood serum
Cortisol is that what from adrenal cortex, to extract is that carbohydate metabolism is had to the cortex hormone of aadrenaline of pretending use most, belongs to a kind of of glucocorticoid.The activation of chronic psychological stress inducing hypothalamic effects-Pituitary Adrenal (hypothalamic-pituitary-adrenal, HPA) axle is found in research, and the one-step inducing cortisol levels of going forward side by side increases.Whether successfully cortisol levels increase can be used as judging whether psychological stress experiment causes hpa axis activation, be to judge psychological stress experiment important indicator.After rotation test, get the mensuration that rat blood serum has carried out cortisol levels, the results are shown in Table 1.
Table 1 is respectively organized the comparison of rat blood serum Determination of cortisol
| Group | Cortisol |
| cont | 48.514±4.39 |
| PS | 125.34±5.12** |
Compare * P<0.05, * * P<0.01 with cont group
Result shows: the rat Cortisol level afterwards of rotating obviously raises, and the embodiment of the present invention is effectively reliable.
5.3 respectively organize spacious Comparison of experiment results of rat
Spacious experiment (Open field test) is a kind of method of evaluation experimental animal independent behaviour, exploratory behavior and tensity in strange environment.With the occurrence frequency of laboratory animal some behavior among novel environment and duration etc., independent behaviour and the exploratory behavior of reaction experiment animal in foreign environment, reacts its tensity to urinate just number of times.By spacious experiment, observed the variation that gives the rat center lattice time of staying, fecal grains afterwards, wears lattice number of times (horizontal score), upright number of times (vertical score), PTS.
5.3.1 respectively organize the time of staying comparison of rat center lattice, in Table 2.
Table 2 is respectively organized the time of staying comparison of rat center lattice
| Group | The center lattice time of staying (s) |
| cont | 21.9760±3.29110 |
| PS | 9.3360±3.17366** |
Compare * P<0.05, * * P<0.01 with cont group
5.3.2 respectively organize the comparison of rat fecal grains, in Table 3.
Table 3 is respectively organized the comparison of rat fecal grains
| Group | Fecal grains |
| cont | 1.3333±0.18191 |
| PS | 3.0000±0.27735* |
Comparing difference not statistically significant between each group.
5.3.3 respectively organize rat and wear lattice number of times (horizontal score) relatively, in Table 4.
Table 4 is respectively organized rat and is worn lattice number of times (horizontal score) relatively
| Group | Wear lattice number of times |
| cont | 47.6000±2.69444 |
| PS | 31.2000±2.43721** |
Compare * P<0.05, * * P<0.01 with cont group
5.3.4 respectively organize the upright number of times of rat (vertical score) relatively, in Table 5.
Table 5 is respectively organized the upright number of times of rat (vertical score) relatively
| Group | Upright number of times |
| cont | 11.75±3.9237525 |
| PS | 31.333333±4.3333333** |
Compare * P<0.05, * * P<0.01 with cont group
(5) respectively organize the comparison of spacious experiment PTS of rat, in Table 6.
Table 6 is respectively organized the comparison of spacious experiment PTS of rat
| Group | Total points |
| cont | 45.666667±4.6666667 |
| PS | 62.75±3.1983068 ** |
Compare * P<0.05, * * P<0.01 with cont group.
Claims (2)
1. a rotation tracking system that causes mouse generation stress reaction, it is characterized in that: be provided with support (1), on support (1), be fixed with motor (2) and motor control assembly (3), the output shaft (4) of motor (2) joins by bearing (5) and horizontally disposed rotation platform (6), on rotation platform (6), be fixed with a plurality of mouse cages (7), described mouse cage (7) is divided into two-layer up and down by grid (8), under be provided with metabolism disposable box (9).
2. the rotation tracking system that causes mouse generation stress reaction according to claim 1, is characterized in that: described a plurality of mouse cages (7) are uniform along rotation platform (6) circumferencial direction.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105340773A (en) * | 2015-12-09 | 2016-02-24 | 河南中医学院 | Experimental facility used for experimental animal simple chronic psychological stress |
| CN106205262A (en) * | 2016-08-03 | 2016-12-07 | 广西大学 | Electromechanical regulation rotates and round translation test experiments machine |
| CN106489759A (en) * | 2016-11-25 | 2017-03-15 | 合肥工业大学 | A kind of rotary mouse anti-reflecting fatigue laboratory table |
| CN108012951A (en) * | 2017-12-12 | 2018-05-11 | 西安医学院 | A kind of rat integrated motion treadmill |
| CN112167091A (en) * | 2020-11-12 | 2021-01-05 | 公安部昆明警犬基地 | Stress desensitization training equipment for police dogs |
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| US20050066910A1 (en) * | 2002-07-25 | 2005-03-31 | The Regents Of The University Of California | Animal cage behavior system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105340773A (en) * | 2015-12-09 | 2016-02-24 | 河南中医学院 | Experimental facility used for experimental animal simple chronic psychological stress |
| CN105340773B (en) * | 2015-12-09 | 2018-07-24 | 河南中医学院 | A kind of experimental provision for experimental animal lichen simplex psychological stress |
| CN106205262A (en) * | 2016-08-03 | 2016-12-07 | 广西大学 | Electromechanical regulation rotates and round translation test experiments machine |
| CN106489759A (en) * | 2016-11-25 | 2017-03-15 | 合肥工业大学 | A kind of rotary mouse anti-reflecting fatigue laboratory table |
| CN108012951A (en) * | 2017-12-12 | 2018-05-11 | 西安医学院 | A kind of rat integrated motion treadmill |
| CN112167091A (en) * | 2020-11-12 | 2021-01-05 | 公安部昆明警犬基地 | Stress desensitization training equipment for police dogs |
| CN112167091B (en) * | 2020-11-12 | 2024-01-30 | 公安部昆明警犬基地 | Police dog stress desensitization training equipment |
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Application publication date: 20141015 |