CN104091962B - The method of automatic structure stable electrical catalysis bacterial biof iotalm - Google Patents
The method of automatic structure stable electrical catalysis bacterial biof iotalm Download PDFInfo
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- CN104091962B CN104091962B CN201410291215.3A CN201410291215A CN104091962B CN 104091962 B CN104091962 B CN 104091962B CN 201410291215 A CN201410291215 A CN 201410291215A CN 104091962 B CN104091962 B CN 104091962B
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Abstract
The present invention relates to a kind of method of automatic structure stable electrical catalysis bacterial biof iotalm, step is as follows: first assemble microorganism electrochemical reactor and connecting circuit, the first via is fixed resistance of connecting between the anode and the cathode, and the second tunnel is the working contact accessing adjustable DC power supply, ammeter and cycle time relay at anode and negative electrode, then anode room is injected bacterial growth media liquid and is inoculated bacterium source, connect the first via and pretrigger is carried out to microbe anode, then connect the second road adjustable DC power supply progressively to increase progressively with the speed set according to the pulse current between anode potential change control anode and negative electrode, when anode potential is increased to limit value, pulse current reduces by 40% ~ 60% at once and again enters new round growth stimulation, until pulse current value reaches and is stabilized in current settings limit value reach more than 10 hours, electro-catalysis bacterial biof iotalm has built, this electro-catalysis bacterial biof iotalm can in stable operation under comparatively high workload electric current.
Description
Technical field
The present invention relates to a kind of method of automatic structure stable electrical catalysis bacterial biof iotalm, belong to microorganism electrochemical reactor technology field.
Background technology
Microorganism electrochemical reactor comprises microbiological fuel cell, microorganism electrolysis cell and microorganism electrochemical sensor.In microorganism electrochemical reactor, the electro catalytic activity and the operation stability that are attached to the electro-catalysis bacterial biof iotalm of electrode surface are the critical components determining reactor performance, the electro-catalysis bacterial biof iotalm of stability and high efficiency can improve the energy efficiency of microbiological fuel cell and microorganism electrolysis cell, improves the sensitivity of microorganism electrochemical sensor signal response.Electro-catalysis bacterial biof iotalm is the cell accumulation layer with certain space structure jointly formed by the electrogenesis bacterium with complete vital metabolic function (sometimes also comprising non-electrogenesis bacterium) cell and the outer material of born of the same parents.On the electrode surface of bio-electrochemical reactor, bacterial biof iotalm has reaction substrate oxidation Decomposition, and the electron transmission that oxidizing process discharges is formed the electrochemical catalysis function of electric current to electrode.
At present, the method building stable electrical catalysis bacterial biof iotalm has two kinds.The first is the construction method of permanent extrernal resistance pattern, key step has: microbionation source is inoculated in the anode chamber of reactor by (1), and microbionation source comprises the mud containing electrogenesis bacterium taken from ambient deposition thing, Sewage Biological Treatment factory and the cultivation bacterium etc. containing electrogenesis bacterium cultivating out in laboratory conditions; (2) connected by certain certain value external resistance and negative electrode by anode, form closed circuit, the adaptability growth utilizing the bacterium being inoculated into anode chamber to carry out at the electrode surface, forms electro-catalysis bacterial biof iotalm.
The second is the construction method of permanent electrode potential pattern, key step has: microbionation source is inoculated in the anode chamber of reactor by (1), and microbionation source comprises the mud containing electrogenesis bacterium taken from ambient deposition thing, Sewage Biological Treatment factory and the cultivation bacterium etc. containing electrogenesis bacterium cultivating out in laboratory conditions; (2) utilize potentiostat and reference electrode to control anode potential and keep a certain steady state value, make the bacterium be inoculated in anode chamber carry out adaptability growth at electrode surface, form electro-catalysis bacterial biof iotalm.Although the electrochemical control method of two kinds of methods is different, two kinds of methods are in fact all utilize the adaptability of bacterium antianode electromotive force to grow, and reach the object building electro-catalysis bacterial biof iotalm.
Above two kinds of differences building the method for electro-catalysis bacterial biof iotalm are: in first method, the electromotive force of dynamic change that what bacterium adapted to is.According to current disclosed data, utilize first method to build in electro catalytic activity biomembrane process at anode surface, anode potential (relative to standard hydrogen electrode) all changes usually in the scope of about+0.5V ~-0.3V.In the second approach, what bacterium adapted to is constant anode potential, in biofilm formation process, usually anode potential is remained on some fixed numbers, according to related data, in the second construction method, be also all that a selected constant anode potential cultivates anode biomembrane in the scope of+0.5V ~-0.3V (relative to standard hydrogen electrode) substantially.
Utilize the electro-catalysis bacterial biof iotalm that electromotive force adaptive method builds, ubiquity is not enough as follows: the maximal work electric current that (1) electrode can reach is less, and electro-catalysis efficiency is low; (2) electro-catalysis bacterial biof iotalm is to poor compared with the ability to bear of high workload electric current, when by the operating current of electrode close to or when exceeding its limiting current, electrode can by rapid hyperpolarization, hyperpolarization can cause anode potential (to be usually no more than 10 minutes) at short notice and just move on to more than+1.2V (relative to standard hydrogen electrode), anode potential high like this can cause irreversible injury to bacterium itself, cause electro-catalysis bacterial biof iotalm to be destroyed, lose catalysis.In a word, utilize the electro-catalysis bacterial biof iotalm that electromotive force adjustment procedure builds, it is less that ubiquity catalytic activity, to the shortcoming of high workload electric current bad adaptability, the electro-catalysis bacterial biof iotalm built, even if when nutriment abundance, also cannot continous-stable work under the operating current close to its limiting current level.Therefore, develop the construction method having higher catalytic activity and have the electro-catalysis bacterial biof iotalm of operation stability under compared with high workload electric current, the bio-electrochemical reactor for exploitation stability and high efficiency has important technical meaning and application and popularization value widely.
Summary of the invention
The object of the invention is to, overcome problems of the prior art, provide a kind of method of automatic structure stable electrical catalysis bacterial biof iotalm, the electro-catalysis bacterial biof iotalm of structure can in stable operation under comparatively high workload electric current.
For solving above technical problem, the method of a kind of automatic structure stable electrical catalysis bacterial biof iotalm of the present invention, in turn include the following steps: (1) assemble microorganism electrochemical reactor, described microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed, (2) connecting circuit element, circuit element comprises selector switch, fixed resistance, reference electrode, ammeter, cycle time relay and adjustable DC power supply, the common end of described selector switch is connected with described positive plate, first selecting side and the described fixed resistance of described selector switch are connected and are connected with described minus plate afterwards, second selecting side of described selector switch is connected with the P1 output of described adjustable DC power supply, the P2 output of described adjustable DC power supply successively with described ammeter, working contact and the described minus plate of cycle time relay are in series, the coil of described cycle time relay is connected to the two ends of auxiliary DC power supply by auxiliary switch, (3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to described microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in described anode chamber, inoculation contains the microbial bacteria source of electrogenesis bacterium again, then described selector switch is allocated to and connects with the first selecting side, anode is connected with cathodic electricity by fixed resistance, pretrigger is carried out to microbe anode, (4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 2 ~ 5 days and is less than 0.1V, second stage is the quick decline stage, and anode potential declined 0.2V ~ 0.6V in 10 ~ 20 hours, phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches more than 10 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes, (5) connecting described auxiliary switch, that the coil of cycle time relay is obtained is electric, regulates the make-and-break time ratio of described cycle time relay for (0.5 ~ 1) second: 1 second, described selector switch is pushed and to connect with the second selecting side and to start described adjustable DC power supply, between described positive plate and described minus plate, form pulse current, described adjustable DC power supply is progressively increased progressively with the speed of setting by pulse current described in adjustment output voltage control, simultaneously microbe anode described in continuous measurement relative to the electrode potential of described reference electrode as the feedback control signal of described adjustable DC power supply, along with progressively increasing progressively of pulse current, the electrode potential of described microbe anode also progressively raises, and the degree of polarization of microbe anode increases, (6), when the electrode potential of described microbe anode is elevated to anode potential limit value, described adjustable DC power supply is reduced to 40% ~ 60% of currency at once by adjustment output voltage control pulse current, and the electrode potential of described microbe anode decreases, then with reduce after current value for ground zero, described adjustable DC power supply again control impuls electric current with setting speed progressively increase progressively, until the electrode potential of described microbe anode is elevated to anode potential limit value again, (7) (6) step operation is repeatedly carried out, the withstanding current capability of described microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, and electro-catalysis bacterial biof iotalm has built.
Relative to prior art, the present invention achieves following beneficial effect: (1) circuit of the present invention provides two loops, when selector switch be allocated to be connected with the first selecting side time, anode is connected with negative electrode by fixed resistance, microbe anode obtains pretrigger, after three phases, electro-catalysis bacterial biof iotalm Primary Construction completes; When selector switch be allocated to be connected with the second selecting side time, connect auxiliary switch, the coil of cycle time relay is in obtain electricity condition simultaneously, and the working contact of cycle time relay carries out the break-make circulated, and realizes the stimulation of pulse current source to microbe anode.(2) within the scope of anode potential limit value, the pulse current antianode electro-catalysis bacterial biof iotalm progressively increased progressively is utilized to stimulate, can realize carrying out high galvanism to electro-catalysis bacterial biof iotalm on the one hand, on the other hand, use pulse current, the electricity currency is no more than 1 second, otherwise can cause the hyperpolarization of microbe anode, the electric current break time is not more than 1 second, otherwise does not reach the abundant galvanism to microbe anode; By regulating suitable duty ratio, the damage can effectively avoiding electro-catalysis bacterial biof iotalm to cause because of anode hyperpolarization, makes electro-catalysis bacterial biof iotalm under high electric current environment, can constantly produce the adaptability to high electric current.(3) the power of electro-catalysis bacterial biof iotalm catalytic activity shows as the producible catalytic current size of biomembrane under certain potential range and ambient condition, and catalytic current is large, then catalytic activity is strong; Of the present invention according to anode potential control impuls galvanism method compared to electromotive force adjustment procedure, the catalytic activity of electro-catalysis bacterial biof iotalm can be enhanced about more than once.(4) utilize of the present invention according to anode potential control impuls galvanism method, the development process of electro-catalysis bacterial biof iotalm at anode surface can be accelerated, and increase electro-catalysis bacterial membrane at the coverage of anode surface and biofilm thickness, the work operation stability of electro-catalysis bacterial biof iotalm is strengthened.(5) of the present invention according to anode potential control impuls galvanism method, be not limited to specific bacterial origin and bacterial groups, utilize different environment bacterium sources, comprise natural sediment and sludge sewage in varying environment, all can in bio-electrochemical reactor, utilize the present invention to construct performance and be better than the electro-catalysis bacterial biof iotalm that electromotive force adjustment procedure obtains.(6) the present invention utilizes adjustable DC Energy control pulse current progressively to increase progressively with the speed of setting, improves automaticity, reduces the labour intensity of operator.(7) when anode potential is elevated to anode potential limit value, adjustable DC power supply at once control impuls electric current significantly reduces, both avoided electro-catalysis bacterial biof iotalm because of anode hyperpolarization cause damage, also make electro-catalysis bacterial biof iotalm obtain the stimulation of high current, strengthen electro-catalysis bacterial biof iotalm to the adaptive capacity of high electric current.
As preferred version of the present invention, described reference electrode is saturated calomel electrode, described anode potential limit value is-0.3V ~-0.1V, and the ascending rate of described pulse current is (0.03 ~ 0.3) mA/ minute, and described current settings limit value is every square centimeter of annode area 1mA.Anode potential limit value gets-0.3V ~-0.1V both can avoid microbe anode generation hyperpolarization, can ensure that again electro-catalysis bacterial biof iotalm is subject to the stimulation of limiting current as far as possible, ensured the effect of pulse current stimulating, improved biomembranous structure efficiency; The ascending rate of pulse current is the efficiency that (0.03 ~ 0.3) both ensure that stimulation for mA/ minute, has taken into account again biomembranous tolerance; When pulse current reaches every square centimeter of annode area 1mA, the withstanding current capability of electro-catalysis bacterial biof iotalm is better than the biomembrane adopting traditional permanent extrernal resistance or permanent electrode potential mode construction greatly, and it is excessive that current settings limit value then realizes difficulty higher than this value.
As preferred version of the present invention, raw material components and the weight content of described bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=(0.05 ~ 0.15): (0.05 ~ 0.15): (0.05 ~ 0.15): (0.05 ~ 0.15): (2.0 ~ 3.0): (1.0 ~ 2.0): (0.5 ~ 1.0): (0.05 ~ 0.15): (0.001 ~ 0.005): (0.001 ~ 0.005): (0.001 ~ 0.005): (0.05 ~ 0.1): (1.6 ~ 3.2): 1000.Potassium chloride, sodium chloride, calcium chloride, magnesium chloride, sodium acid carbonate, ammonium chloride, sodium dihydrogen phosphate and magnesium sulfate are constant inorganic salts, be used on the one hand supplementing potassium, sodium, calcium, magnesium, carbon, the nitrogen phosphate and sulfur element needed for bacterial growth, on the other hand, also there is ionic strength and the conductive capability of regulation culture liquid; As above proportioning makes nutrient solution keep total ion concentration to be 30mM ~ 40mM, and conductivity is 1.5mS/cm ~ 3.0mS/cm; Wherein sodium acid carbonate and sodium dihydrogen phosphate are except the inorganic carbon source being used separately as bacterial growth and phosphorus source, also have the effect of the pH of buffering nutrient solution.Ferrous sulfate heptahydrate, tetrahydrate manganese chloride and Sodium Molybdate Dihydrate supplement trace elements iron, manganese and molybdenum needed for nutrient solution respectively.Yeast juice is the growth factor in nutrient solution; Sodium acetate, as organic substrates, is the energy substance needed for bacterial growth, and above component and weight ratio can make electrogenesis bacterium under limiting current stimulates at anode advolution preferably.
As preferred version of the present invention, described cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
As preferred version of the present invention, described microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in city domestic sewage treatment plant activated sludge or fresh water environment deposit, and inoculum concentration is by inoculating described activated sludge or described deposit 50 ~ 100 grams in bacterial growth media liquid often liter described.
As preferred version of the present invention, described adjustable DC power supply comprises serial communication interface, level transferring chip, master chip, D/A conversion chip and operational amplifier, described serial communication interface be connected with described level transferring chip carry out serial ports receive with send data, described level transferring chip and described master chip carry out serial communication and are connected and realize the parameter-embedded write of master chip or amendment, described master chip is to described D/A conversion chip tranmitting data register control signal and digital voltage control signal, the signal output part of described D/A conversion chip is connected with the signal input part of described operational amplifier, the signal output part of described operational amplifier is connected with described P2 output, described P1 output is by current-limiting resistance ground connection, described master chip is exported by control clock signal and digital voltage signal exports the adjustment peak value of change in voltage and the speed of change in voltage, the digital voltage signal received is converted to voltage analog signal and sends to described operational amplifier by signal output part by described D/A conversion chip, the carrying out amplification to the voltage analog signal received and improve after its driving force and export of described operational amplifier.Master chip is exported by control clock signal and digital voltage signal exports the control finally realized change in voltage peak value and rate of change between P1 output and P2 output, thus realizes the size of pulse current and the control of rate of change between antianode plate and minus plate.
As preferred version of the present invention, described serial communication interface is DB9 serial port communication nine kinds of needles interface, described level transferring chip is MAX232 cake core, described master chip is STC12C5A60S2 type single-chip microcomputer, described D/A conversion chip is MAX517 type conversion chip, and described operational amplifier is LM358 type operational amplifier.
As preferred version of the present invention, No. 2 pin of described serial communication interface and No. 3 pin access No. 14 pin of described level transferring chip and No. 13 pin respectively to carry out serial ports and receives and send data, and No. 11 pin of described level transferring chip and No. 12 pin are connected with No. 10 pin with No. 11 pin of described master chip respectively and carry out serial communication; No. 39 pin of described master chip are connected with No. 3 pin of described D/A conversion chip and connect the pull-up resistor of 10K, No. 38 pin of described master chip are connected with No. 4 pin of described D/A conversion chip and connect the pull-up resistor of 10K, clock signal is transmitted, transmitting digital voltage signal between No. 38 pin of described master chip and No. 4 pin of described D/A conversion chip between No. 39 pin of described master chip and No. 3 pin of described D/A conversion chip; No. 1 pin of described D/A conversion chip accesses No. 3 pin transfer overvoltage analog signals of described operational amplifier, and No. 1 pin of described operational amplifier is with No. 2 pin and be connected with described P2 output after connecing.
As preferred version of the present invention, No. 1 pin and No. 3 pin of described level transferring chip are interconnected by 1 μ F filter capacitor, No. 2 pin are connected with Vcc by 1 μ F filter capacitor, No. 4 pin and No. 5 pin are interconnected by 1 μ F filter capacitor, No. 6 pin pass through 1 μ F filter capacitor ground connection, No. 7 pin and No. 8 pin short circuits, and No. 9 pin are unsettled, No. 10 pin and No. 15 pin ground connection, No. 16 pin of described level transferring chip meet Vcc and by 1 μ F filter capacitor ground connection; 10 μ F filter capacitors and 10K resistance form reset pin No. 9 pin that rc reset circuit connects described master chip, complete electrification reset, No. 18 pin of described master chip, No. 19 pin are connected by crystal oscillator and respectively by 30pF filter capacitor ground connection, No. 20 pin ground connection of described master chip, No. 40 pin meet Vcc and by filter capacitor ground connection; No. 2 pin of described D/A conversion chip, No. 5 pin, No. 6 equal ground connection of pin, No. 7 pin and No. 8 pin meet Vcc; No. 4 pin ground connection of described operational amplifier, No. 8 pin meet Vcc and by filter capacitor ground connection.
As preferred version of the present invention, described reference electrode accesses No. 7 pin of described master chip.Master chip directly according to reference electrode potential, can calculate the electrode potential of microbe anode relative to reference electrode, and as the change of feedback signal control impuls electric current.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further detailed explanation, and accompanying drawing only provides reference and explanation use, is not used to limit the present invention.
Fig. 1 is the circuit connection diagram of microorganism electrochemical reactor.
Fig. 2 is the schematic diagram of adjustable DC power supply.
Fig. 3 is the anode potential variation diagram of microbe anode in pre-boot process.
Fig. 4 is the electro-catalysis bacterial biof iotalm developed without limited pulses galvanism.
Fig. 5 is anode potential and the pulse current change procedure figure of microorganism electrochemical reactor.
Fig. 6 is the electro-catalysis bacterial biof iotalm developed through limited pulses galvanism.
In figure: QS1. selector switch; QS2. auxiliary switch; R
0. fixed resistance; DV1. D.C. regulated power supply; DV2. auxiliary DC power supply; A. ammeter; KT. cycle time relay; RE. reference electrode.
Embodiment
Figure 1 shows that the circuit connection diagram of microorganism electrochemical reactor of the present invention, circuit element comprises selector switch QS1, fixed resistance R
0, reference electrode RE, ammeter A, the on/off time is than at (0.1s ~ 10s): cycle time relay KT adjustable in (0.1s ~ 10s) scope and adjustable DC power supply, the common end of selector switch QS1 is connected with positive plate, first selecting side of selector switch QS1 and fixed resistance R
0be connected with minus plate after series connection, second selecting side of selector switch QS1 is connected with the P1 output of adjustable DC power supply, the P2 output of adjustable DC power supply is in series with the working contact of ammeter, cycle time relay KT and minus plate successively, and the coil of cycle time relay KT is connected to the two ends of auxiliary DC power supply DV2 by auxiliary switch QS2.D.C. regulated power supply DV1 provides fundamental voltage to adjustable DC power supply, and reference electrode RE selects saturated calomel electrode, and anode potential is measured monitoring and can be carried out separately, also directly can get signal measurement by adjustable DC power supply.Positive plate area is 1.5cm × 3.0cm, and minus plate area is 5.0cm × 10.0cm.
As shown in Figure 2, adjustable DC power supply comprises serial communication interface, level transferring chip, master chip, D/A conversion chip and operational amplifier, serial communication interface be connected with level transferring chip carry out serial ports receive with send data, level transferring chip and master chip carry out serial communication and are connected and realize the parameter-embedded write of master chip or amendment, master chip is to D/A conversion chip tranmitting data register control signal and digital voltage control signal, the signal output part of D/A conversion chip is connected with the signal input part of operational amplifier, the signal output part of operational amplifier is connected with P2 output, P1 output is by current-limiting resistance ground connection, master chip is exported by control clock signal and digital voltage signal exports the adjustment peak value of change in voltage and the speed of change in voltage, the digital voltage signal received is converted to voltage analog signal and sends to operational amplifier by signal output part by D/A conversion chip, the carrying out amplification to the voltage analog signal received and improve after its driving force and export of operational amplifier.Master chip is exported by control clock signal and digital voltage signal exports the control finally realized change in voltage peak value and rate of change between P1 output and P2 output, thus realizes the size of pulse current and the control of rate of change between antianode plate and minus plate.
Serial communication interface is preferably DB9 serial port communication nine kinds of needles interface, level transferring chip is preferably MAX232 cake core, master chip is preferably STC12C5A60S2 type single-chip microcomputer, and D/A conversion chip is preferably MAX517 type conversion chip, and operational amplifier is preferably LM358 type operational amplifier.
No. 2 pin of serial communication interface and No. 3 pin access No. 14 pin of level transferring chip and No. 13 pin respectively to carry out serial ports and receives and send data, and No. 11 pin of level transferring chip and No. 12 pin are connected with No. 10 pin with No. 11 pin of master chip respectively and carry out serial communication; No. 1 pin and No. 3 pin of level transferring chip are interconnected by 1 μ F filter capacitor, No. 2 pin are connected with Vcc by 1 μ F filter capacitor, No. 4 pin and No. 5 pin are interconnected by 1 μ F filter capacitor, No. 6 pin are by 1 μ F filter capacitor ground connection, No. 7 pin and No. 8 pin short circuits, No. 9 pin are unsettled, No. 10 pin and No. 15 pin ground connection, and No. 16 pin of level transferring chip meet Vcc and by 1 μ F filter capacitor ground connection.
No. 39 pin of master chip are connected with No. 3 pin of D/A conversion chip and connect the pull-up resistor of 10K, and No. 38 pin of master chip are connected with No. 4 pin of D/A conversion chip and connect the pull-up resistor of 10K; Clock signal is transmitted, transmitting digital voltage signal between No. 38 pin of master chip and No. 4 pin of D/A conversion chip between No. 39 pin of master chip and No. 3 pin of D/A conversion chip.10 μ F filter capacitors and 10K resistance form reset pin No. 9 pin that rc reset circuit connects master chip, complete electrification reset; No. 7 pin of reference electrode access master chip, No. 18 pin of master chip, No. 19 pin are connected by crystal oscillator and respectively by 30pF filter capacitor ground connection, No. 20 pin ground connection of master chip, and No. 40 pin meet Vcc and by filter capacitor ground connection.
No. 3 pin transfer overvoltage analog signals of No. 1 pin access operational amplifier of D/A conversion chip, No. 2 pin of D/A conversion chip, No. 5 pin, No. 6 equal ground connection of pin, No. 7 pin and No. 8 pin meet Vcc.
No. 1 pin of operational amplifier is with No. 2 pin and be connected with P2 output after connecing, No. 4 pin ground connection of operational amplifier, and No. 8 pin meet Vcc and by filter capacitor ground connection.
Embodiment one
The present invention builds the method for stable electrical catalysis bacterial biof iotalm automatically, in turn includes the following steps:
(1) assemble microorganism electrochemical reactor, microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed.
(2) connecting circuit element as shown in Figure 1 and Figure 2.
(3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in anode room, selector switch QS1, containing the microbial bacteria source of electrogenesis bacterium, is then allocated to connect with the first selecting side and makes anode by fixed resistance R by inoculation again
0be connected with cathodic electricity, pretrigger is carried out to microbe anode.
Raw material components and the weight content of bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=0.08: 0.07: 0.07: 0.08: 2.5: 1.5: 0.7: 0.10:0.003:0.003:0.003:0.08:2.4:1000.Cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
Microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in city domestic sewage treatment plant activated sludge, and inoculum concentration is by inoculation of activated-sludge 80 grams in often liter of bacterial growth media liquid.
(4) the anode potential change of continuous measurement microbe anode in pre-boot process, as shown in Figure 3, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 65 hours and is less than 0.1V; Second stage is the quick decline stage, and anode potential declined 0.6V in 20 hours; Phase III is the smooth change stage, the amplitude stabilization of anode potential change reaches 15 hours within ± 0.03V, complete when the anode potential change arrival phase III is microbe anode pretrigger, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes, and electro-catalysis bacterial biof iotalm now as shown in Figure 4.
(5) connecting auxiliary switch QS2, that the coil of cycle time relay KT is obtained is electric, and the make-and-break time ratio regulating cycle time relay KT is 1 second: 1 second; Pushed by selector switch and to connect with the second selecting side and to start adjustable DC power supply, between positive plate and minus plate, form pulse current, adjustable DC power supply is progressively increased progressively with the speed of setting by adjustment output voltage control pulse current; Simultaneously continuous measurement microbe anode is relative to the electrode potential of reference electrode as the feedback control signal of adjustable DC power supply, and along with progressively increasing progressively of pulse current, the electrode potential of microbe anode also progressively raises, and the degree of polarization of microbe anode increases.
(6), when the electrode potential of microbe anode is elevated to anode potential limit value, adjustable DC power supply is reduced to 50% of currency at once by adjustment output voltage control pulse current, and the electrode potential of microbe anode decreases; Then with reduce after current value for ground zero, adjustable DC power supply again control impuls electric current progressively increases progressively with the speed of setting, until the electrode potential of microbe anode is elevated to anode potential limit value again, anode potential limit value gets-0.3V, and the ascending rate of pulse current is 0.03mA/ minute.
(7) repeatedly carry out (6) step operation, the withstanding current capability of microbe anode progressively strengthens, as shown in Figure 5, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, electro-catalysis bacterial biof iotalm has built, and current settings limit value is every square centimeter of annode area 1mA, and the electro-catalysis bacterial biof iotalm developed through limited pulses galvanism as shown in Figure 6.
Embodiment two
The present invention builds the method for stable electrical catalysis bacterial biof iotalm automatically, in turn includes the following steps:
(1) assemble microorganism electrochemical reactor, microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed.
(2) connecting circuit element as shown in Figure 1 and Figure 2.
(3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in anode room, inoculation contains the microbial bacteria source of electrogenesis bacterium again, then being allocated to by selector switch QS1 to connect with the first selecting side makes anode be connected with cathodic electricity by fixed resistance R0, carries out pretrigger to microbe anode.
Raw material components and the weight content of bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=0.05: 0.05: 0.05: 0.05: 2.0: 1.0: 0.5: 0.05:0.001:0.001:0.001:0.05:1.6:1000.Cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
Microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in fresh water environment deposit, and inoculum concentration inoculates deposit 50 grams by often liter of bacterial growth media liquid.
(4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 2 days and is less than 0.1V; Second stage is the quick decline stage, and anode potential declined 0.2V in 10 hours; Phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches 10 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes.
(5) connecting auxiliary switch QS2, that the coil of cycle time relay KT is obtained is electric, and the make-and-break time ratio regulating cycle time relay KT is 0.5 second: 1 second; Pushed by selector switch and to connect with the second selecting side and to start adjustable DC power supply, between positive plate and minus plate, form pulse current, adjustable DC power supply is progressively increased progressively with the speed of setting by adjustment output voltage control pulse current; Simultaneously continuous measurement microbe anode is relative to the electrode potential of reference electrode as the feedback control signal of adjustable DC power supply, and along with progressively increasing progressively of pulse current, the electrode potential of microbe anode also progressively raises, and the degree of polarization of microbe anode increases.
(6), when the electrode potential of microbe anode is elevated to anode potential limit value, adjustable DC power supply is reduced to 40% of currency at once by adjustment output voltage control pulse current, and the electrode potential of microbe anode decreases; Then with reduce after current value for ground zero, adjustable DC power supply again control impuls electric current progressively increases progressively with the speed of setting, until the electrode potential of microbe anode is elevated to anode potential limit value again, anode potential limit value gets-0.2V, and the ascending rate of pulse current is 0.06mA/ minute.
(7) repeatedly carry out (6) step operation, the withstanding current capability of microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, electro-catalysis bacterial biof iotalm has built, and current settings limit value is every square centimeter of annode area 1mA.
Embodiment three
The present invention builds the method for stable electrical catalysis bacterial biof iotalm automatically, in turn includes the following steps:
(1) assemble microorganism electrochemical reactor, microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed.
(2) connecting circuit element as shown in Figure 1 and Figure 2.
(3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in anode room, selector switch QS1, containing the microbial bacteria source of electrogenesis bacterium, is then allocated to connect with the first selecting side and makes anode by fixed resistance R by inoculation again
0be connected with cathodic electricity, pretrigger is carried out to microbe anode.
Raw material components and the weight content of bacterial growth media liquid are as follows, potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=0.15: 0.15: 0.15: 0.15: 3.0: 2.0: 1.0: 0.15:0.005:0.005:0.005:0.1:3.2:1000.Cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
Microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in city domestic sewage treatment plant activated sludge, and inoculum concentration is by inoculation of activated-sludge 100 grams in often liter of bacterial growth media liquid.
(4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 7 days and is less than 0.1V; Second stage is the quick decline stage, and anode potential declined 0.6V in 20 hours; Phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches 12 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes.
(5) connecting auxiliary switch QS2, that the coil of cycle time relay KT is obtained is electric, and the make-and-break time ratio regulating cycle time relay KT is 0.7 second: 1 second; Pushed by selector switch and to connect with the second selecting side and to start adjustable DC power supply, between positive plate and minus plate, form pulse current, adjustable DC power supply is progressively increased progressively with the speed of setting by adjustment output voltage control pulse current; Simultaneously continuous measurement microbe anode is relative to the electrode potential of reference electrode as the feedback control signal of adjustable DC power supply, and along with progressively increasing progressively of pulse current, the electrode potential of microbe anode also progressively raises, and the degree of polarization of microbe anode increases.
(6), when the electrode potential of microbe anode is elevated to anode potential limit value, adjustable DC power supply is reduced to 60% of currency at once by adjustment output voltage control pulse current, and the electrode potential of microbe anode decreases; Then with reduce after current value for ground zero, adjustable DC power supply again control impuls electric current progressively increases progressively with the speed of setting, until the electrode potential of microbe anode is elevated to anode potential limit value again, anode potential limit value gets-0.1V, and the ascending rate of pulse current is 0.3mA/ minute.
(7) repeatedly carry out (6) step operation, the withstanding current capability of microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, electro-catalysis bacterial biof iotalm has built, and current settings limit value is every square centimeter of annode area 1mA.
Embodiment four
The present invention builds the method for stable electrical catalysis bacterial biof iotalm automatically, in turn includes the following steps:
(1) assemble microorganism electrochemical reactor, microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed.
(2) connecting circuit element as shown in Figure 1 and Figure 2.
(3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in anode room, selector switch QS1, containing the microbial bacteria source of electrogenesis bacterium, is then allocated to connect with the first selecting side and makes anode by fixed resistance R by inoculation again
0be connected with cathodic electricity, pretrigger is carried out to microbe anode.
Raw material components and the weight content of bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=0.05: 0.15: 0.05: 0.15: 2.5: 1.0: 1.0: 0.15:0.001:0.005:0.001:0.1:2.0:1000.Cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
Microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in fresh water environment deposit, and inoculum concentration inoculates deposit 70 grams by often liter of bacterial growth media liquid.
(4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 3 days and is less than 0.1V; Second stage is the quick decline stage, and anode potential declined 0.4V in 16 hours; Phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches 13 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes.
(5) connecting auxiliary switch QS2, that the coil of cycle time relay KT is obtained is electric, and the make-and-break time ratio regulating cycle time relay KT is 1 second: 1 second; Pushed by selector switch and to connect with the second selecting side and to start adjustable DC power supply, between positive plate and minus plate, form pulse current, adjustable DC power supply is progressively increased progressively with the speed of setting by adjustment output voltage control pulse current; Simultaneously continuous measurement microbe anode is relative to the electrode potential of reference electrode as the feedback control signal of adjustable DC power supply, and along with progressively increasing progressively of pulse current, the electrode potential of microbe anode also progressively raises, and the degree of polarization of microbe anode increases.
(6), when the electrode potential of microbe anode is elevated to anode potential limit value, adjustable DC power supply is reduced to 50% of currency at once by adjustment output voltage control pulse current, and the electrode potential of microbe anode decreases; Then with reduce after current value for ground zero, adjustable DC power supply again control impuls electric current progressively increases progressively with the speed of setting, until the electrode potential of microbe anode is elevated to anode potential limit value again, anode potential limit value gets-0.3V, and the ascending rate of pulse current is 0.1mA/ minute.
(7) repeatedly carry out (6) step operation, the withstanding current capability of microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, electro-catalysis bacterial biof iotalm has built, and current settings limit value is every square centimeter of annode area 1mA.
Embodiment five
The present invention builds the method for stable electrical catalysis bacterial biof iotalm automatically, in turn includes the following steps:
(1) assemble microorganism electrochemical reactor, microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed.
(2) connecting circuit element as shown in Figure 1 and Figure 2.
(3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in anode room, selector switch QS1, containing the microbial bacteria source of electrogenesis bacterium, is then allocated to connect with the first selecting side and makes anode by fixed resistance R by inoculation again
0be connected with cathodic electricity, pretrigger is carried out to microbe anode.
Raw material components and the weight content of bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=0.09: 0.08: 0.07: 0.09: 2.4: 1.8: 0.9: 0.07:0.002:0.003:0.005:0.07:1.9:1000.Cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
Microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in city domestic sewage treatment plant activated sludge, and inoculum concentration is by inoculation of activated-sludge 90 grams in often liter of bacterial growth media liquid.
(4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 4 days and is less than 0.1V; Second stage is the quick decline stage, and anode potential declined 0.5V in 18 hours; Phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches 18 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes.
(5) connecting auxiliary switch QS2, that the coil of cycle time relay KT is obtained is electric, and the make-and-break time ratio regulating cycle time relay KT is 0.8 second: 1 second; Pushed by selector switch and to connect with the second selecting side and to start adjustable DC power supply, between positive plate and minus plate, form pulse current, adjustable DC power supply is progressively increased progressively with the speed of setting by adjustment output voltage control pulse current; Simultaneously continuous measurement microbe anode is relative to the electrode potential of reference electrode as the feedback control signal of adjustable DC power supply, and along with progressively increasing progressively of pulse current, the electrode potential of microbe anode also progressively raises, and the degree of polarization of microbe anode increases.
(6), when the electrode potential of microbe anode is elevated to anode potential limit value, adjustable DC power supply is reduced to 50% of currency at once by adjustment output voltage control pulse current, and the electrode potential of microbe anode decreases; Then with reduce after current value for ground zero, adjustable DC power supply again control impuls electric current progressively increases progressively with the speed of setting, until the electrode potential of microbe anode is elevated to anode potential limit value again, anode potential limit value gets-0.2V, and the ascending rate of pulse current is 0.2mA/ minute.
(7) repeatedly carry out (6) step operation, the withstanding current capability of microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, electro-catalysis bacterial biof iotalm has built, and current settings limit value is every square centimeter of annode area 1mA.
Embodiment six
The present invention builds the method for stable electrical catalysis bacterial biof iotalm automatically, in turn includes the following steps:
(1) assemble microorganism electrochemical reactor, microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed.
(2) connecting circuit element as shown in Figure 1 and Figure 2.
(3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in anode room, selector switch QS1, containing the microbial bacteria source of electrogenesis bacterium, is then allocated to connect with the first selecting side and makes anode by fixed resistance R by inoculation again
0be connected with cathodic electricity, pretrigger is carried out to microbe anode.
Raw material components and the weight content of bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=0.15: 0.05: 0.15: 0.08: 2.0: 2.0: 0.5: 0.08:0.003:0.004:0.005:0.05:3.2:1000.Cathode solution comprises 50mMK
3fe (CN)
6and 100mMKH
2pO
4.
Microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in city domestic sewage treatment plant activated sludge, and inoculum concentration is by inoculation of activated-sludge 90 grams in often liter of bacterial growth media liquid.
(4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 4 days and is less than 0.1V; Second stage is the quick decline stage, and anode potential declined 0.5V in 16 hours; Phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches 10 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes.
(5) connecting auxiliary switch QS2, that the coil of cycle time relay KT is obtained is electric, and the make-and-break time ratio regulating cycle time relay KT is 1 second: 1 second; Pushed by selector switch and to connect with the second selecting side and to start adjustable DC power supply, between positive plate and minus plate, form pulse current, adjustable DC power supply is progressively increased progressively with the speed of setting by adjustment output voltage control pulse current; Simultaneously continuous measurement microbe anode is relative to the electrode potential of reference electrode as the feedback control signal of adjustable DC power supply, and along with progressively increasing progressively of pulse current, the electrode potential of microbe anode also progressively raises, and the degree of polarization of microbe anode increases.
(6), when the electrode potential of microbe anode is elevated to anode potential limit value, adjustable DC power supply is reduced to 40% of currency at once by adjustment output voltage control pulse current, and the electrode potential of microbe anode decreases; Then with reduce after current value for ground zero, adjustable DC power supply again control impuls electric current progressively increases progressively with the speed of setting, until the electrode potential of microbe anode is elevated to anode potential limit value again, anode potential limit value gets-0.3V, and the ascending rate of pulse current is 0.15mA/ minute.
(7) repeatedly carry out (6) step operation, the withstanding current capability of microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, electro-catalysis bacterial biof iotalm has built, and current settings limit value is every square centimeter of annode area 1mA.
By U.S., MAX232 cake core and MAX517 type conversion chip believe that (MAXIM) company produces, STC12C5A60S2 type single-chip microcomputer is produced by macrocrystalline Science and Technology Ltd., and LM358 type operational amplifier is contained one hundred prestige Electronics Co., Ltd. by Shenzhen and produced.
The foregoing is only the better possible embodiments of the present invention, non-ly therefore limit to scope of patent protection of the present invention.In addition to the implementation, the present invention can also have other execution modes, such as, can adopt other adjustable DC power supply functionally similar.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop in the protection range of application claims.The present invention can pass through without the technical characteristic described or adopt existing techniques in realizing, does not repeat them here.
Claims (10)
1. one kind builds the method for stable electrical catalysis bacterial biof iotalm automatically, it is characterized in that, in turn include the following steps: (1) assemble microorganism electrochemical reactor, described microorganism electrochemical reactor comprises the anode chamber that positive plate is housed and the cathode chamber that minus plate is housed, (2) connecting circuit element, circuit element comprises selector switch, fixed resistance, reference electrode, ammeter, cycle time relay and adjustable DC power supply, the common end of described selector switch is connected with described positive plate, first selecting side and the described fixed resistance of described selector switch are connected and are connected with described minus plate afterwards, second selecting side of described selector switch is connected with the P1 output of described adjustable DC power supply, the P2 output of described adjustable DC power supply successively with described ammeter, working contact and the described minus plate of cycle time relay are in series, the coil of described cycle time relay is connected to the two ends of auxiliary DC power supply by auxiliary switch, (3) microbe anode pretrigger, bacterial growth media liquid injects by anode submergence in anode chamber to described microorganism electrochemical reactor, cathode chamber to microorganism electrochemical reactor injects cathode solution by negative electrode submergence, in described anode chamber, inoculation contains the microbial bacteria source of electrogenesis bacterium again, then described selector switch is allocated to and connects with the first selecting side, anode is connected with cathodic electricity by fixed resistance, pretrigger is carried out to microbe anode, (4) the anode potential change of continuous measurement microbe anode in pre-boot process, anode potential change is divided into three phases successively, and the first stage is the slow decline stage, and anode potential is in a high position common decline in 2 ~ 5 days and is less than 0.1V, second stage is the quick decline stage, and anode potential declined 0.2V ~ 0.6V in 10 ~ 20 hours, phase III is the smooth change stage, and the amplitude stabilization of anode potential change reaches more than 10 hours within ± 0.03V, and be microbe anode pretrigger complete when anode potential change arrives the phase III, the electro-catalysis bacterial biof iotalm Primary Construction of anode completes, (5) connecting described auxiliary switch, that the coil of cycle time relay is obtained is electric, regulates the make-and-break time ratio of described cycle time relay for (0.5 ~ 1) second: 1 second, described selector switch is pushed and to connect with the second selecting side and to start described adjustable DC power supply, between described positive plate and described minus plate, form pulse current, described adjustable DC power supply is progressively increased progressively with the speed of setting by pulse current described in adjustment output voltage control, simultaneously microbe anode described in continuous measurement relative to the electrode potential of described reference electrode as the feedback control signal of described adjustable DC power supply, along with progressively increasing progressively of pulse current, the electrode potential of described microbe anode also progressively raises, and the degree of polarization of microbe anode increases, (6), when the electrode potential of described microbe anode is elevated to anode potential limit value, described adjustable DC power supply is reduced to 40% ~ 60% of currency at once by adjustment output voltage control pulse current, and the electrode potential of described microbe anode decreases, then with reduce after current value for ground zero, described adjustable DC power supply again control impuls electric current with setting speed progressively increase progressively, until the electrode potential of described microbe anode is elevated to anode potential limit value again, (7) (6) step operation is repeatedly carried out, the withstanding current capability of described microbe anode progressively strengthens, until before the electrode potential of microbe anode rises to anode potential limit value, pulse current value reaches and is stabilized in current settings limit value and reaches more than 10 hours, and electro-catalysis bacterial biof iotalm has built.
2. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 1, it is characterized in that: described reference electrode is saturated calomel electrode, described anode potential limit value is-0.3V ~-0.1V, the ascending rate of described pulse current is (0.03 ~ 0.3) mA/ minute, and described current settings limit value is every square centimeter of annode area 1mA.
3. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 1, it is characterized in that, raw material components and the weight content of described bacterial growth media liquid are as follows: potassium chloride: sodium chloride: calcium chloride: magnesium chloride: sodium acid carbonate: ammonium chloride: sodium dihydrogen phosphate: magnesium sulfate: ferrous sulfate heptahydrate: tetrahydrate manganese chloride: Sodium Molybdate Dihydrate: yeast juice: sodium acetate: water=(0.05 ~ 0.15): (0.05 ~ 0.15): (0.05 ~ 0.15): (0.05 ~ 0.15): (2.0 ~ 3.0): (1.0 ~ 2.0): (0.5 ~ 1.0): (0.05 ~ 0.15): (0.001 ~ 0.005): (0.001 ~ 0.005): (0.001 ~ 0.005): (0.05 ~ 0.1): (1.6 ~ 3.2): 1000.
4. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 1, is characterized in that: described cathode solution comprises 50mM
and 100mM
.
5. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 1, it is characterized in that: described microbial bacteria source is the microbial bacteria source containing electrogenesis bacterium in city domestic sewage treatment plant activated sludge or fresh water environment deposit, and inoculum concentration is by inoculating described activated sludge or described deposit 50 ~ 100 grams in bacterial growth media liquid often liter described.
6. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 1, it is characterized in that: described adjustable DC power supply comprises serial communication interface, level transferring chip, master chip, D/A conversion chip and operational amplifier, described serial communication interface be connected with described level transferring chip carry out serial ports receive with send data, described level transferring chip and described master chip carry out serial communication and are connected and realize the parameter-embedded write of master chip or amendment, described master chip is to described D/A conversion chip tranmitting data register control signal and digital voltage control signal, the signal output part of described D/A conversion chip is connected with the signal input part of described operational amplifier, the signal output part of described operational amplifier is connected with described P2 output, described P1 output is by current-limiting resistance ground connection, described master chip is exported by control clock signal and digital voltage signal exports the adjustment peak value of change in voltage and the speed of change in voltage, the digital voltage signal received is converted to voltage analog signal and sends to described operational amplifier by signal output part by described D/A conversion chip, the carrying out amplification to the voltage analog signal received and improve after its driving force and export of described operational amplifier.
7. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 6, it is characterized in that: described serial communication interface is DB9 serial port communication nine kinds of needles interface, described level transferring chip is MAX232 cake core, described master chip is STC12C5A60S2 type single-chip microcomputer, described D/A conversion chip is MAX517 type conversion chip, and described operational amplifier is LM358 type operational amplifier.
8. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 7, it is characterized in that: No. 2 pin of described serial communication interface and No. 3 pin access No. 14 pin of described level transferring chip and No. 13 pin respectively to carry out serial ports and receive and send data, No. 11 pin of described level transferring chip and No. 12 pin are connected with No. 10 pin with No. 11 pin of described master chip respectively and carry out serial communication; No. 39 pin of described master chip are connected with No. 3 pin of described D/A conversion chip and connect the pull-up resistor of 10K, No. 38 pin of described master chip are connected with No. 4 pin of described D/A conversion chip and connect the pull-up resistor of 10K, clock signal is transmitted, transmitting digital voltage signal between No. 38 pin of described master chip and No. 4 pin of described D/A conversion chip between No. 39 pin of described master chip and No. 3 pin of described D/A conversion chip; No. 1 pin of described D/A conversion chip accesses No. 3 pin transfer overvoltage analog signals of described operational amplifier, and No. 1 pin of described operational amplifier is with No. 2 pin and be connected with described P2 output after connecing.
9. the method for automatic structure stable electrical catalysis bacterial biof iotalm according to claim 8, it is characterized in that: No. 1 pin and No. 3 pin of described level transferring chip are interconnected by 1 μ F filter capacitor, No. 2 pin are connected with Vcc by 1 μ F filter capacitor, No. 4 pin and No. 5 pin are interconnected by 1 μ F filter capacitor, No. 6 pin are by 1 μ F filter capacitor ground connection, No. 7 pin and No. 8 pin short circuits, No. 9 pin are unsettled, No. 10 pin and No. 15 pin ground connection, No. 16 pin of described level transferring chip meet Vcc and by 1 μ F filter capacitor ground connection; 10 μ F filter capacitors and 10K resistance form reset pin No. 9 pin that rc reset circuit connects described master chip, complete electrification reset, No. 18 pin of described master chip, No. 19 pin are connected by crystal oscillator and respectively by 30pF filter capacitor ground connection, No. 20 pin ground connection of described master chip, No. 40 pin meet Vcc and by filter capacitor ground connection; No. 2 pin of described D/A conversion chip, No. 5 pin, No. 6 equal ground connection of pin, No. 7 pin and No. 8 pin meet Vcc; No. 4 pin ground connection of described operational amplifier, No. 8 pin meet Vcc and by filter capacitor ground connection.
10. the method for the automatic structure stable electrical catalysis bacterial biof iotalm according to claim 7 or 8 or 9, is characterized in that: described reference electrode accesses No. 7 pin of described master chip.
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| CN103427102A (en) * | 2013-08-30 | 2013-12-04 | 华南理工大学 | Phycomycetes microbial fuel cell and preparation method and application of phycomycetes microbial fuel cell |
| CN103487385A (en) * | 2013-10-16 | 2014-01-01 | 重庆大学 | On-line measurement method of high-efficiency biological membrane photobioreactor |
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| CN102906246A (en) * | 2010-02-23 | 2013-01-30 | 贝克蒂瑞罗博蒂克斯有限责任公司 | Improved microbial fuel cell |
| CN103427102A (en) * | 2013-08-30 | 2013-12-04 | 华南理工大学 | Phycomycetes microbial fuel cell and preparation method and application of phycomycetes microbial fuel cell |
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