CN104087519A - Radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment - Google Patents

Abstract

The invention discloses a radiation-resistant Aspergillus sp. and application thereof in cesium 137 adsorption biological treatment. Sampling in a certain region, screening, separation, screening and physiologic and biochemical identification are performed to obtain the Aspergillus sp. F77 CGMCC No.8382. By utilizing the application of the Aspergillus sp. in cesium 137 adsorption biological treatment, the Aspergillus sp. has the maximal resistance concentrations for Pb<2+>, Zn<2+> and Ni<+>, which can respectively reach 1000 mg/L, 500 mg/L and 500 mg/L; and the resistance concentrations for Co<2+>, Cr<2+> and Hg<2+> take second place, and can respectively reach 200 mg/L. According to the method, the growing thallus can adsorb caesium ions and radiocesium, or the dry thallus can be directly used for adsorption, wherein the maximal adsorption capacity can reach 44.5 mg/g dry thallus. The application of the strain has actual value and functions in Cs 137 adsorption biological treatment.

Description

A kind of radiation hardness aspergillus and the application in the biological treatment of absorption Ce 137 thereof

Technical field

The present invention relates to a kind of microbial strains and be applied to biological adsorption radionuclide technical field, concrete, the technical field that the present invention relates to a kind of radiation hardness aspergillus and apply in radionuclide absorption.

Background technology

Urbanization, industrialization and population growth cause global energy crisis, and Sustainable development demand makes the mankind more and more depend on new forms of energy.Nuclear energy is with its extremely low Carbon emission and huge development potentiality and be more and more subject to the attention of numerous countries.In the time of nuclear energy high speed development, also to global environment, cause huge pressure, make radiocontamination become one of current great environmental problem, the nuclear incident that Chernobyl and Fukushima, Japan Nuclear power plants be representative of particularly take has discharged a large amount of radioactive pollutants to environment, cause the radiocontamination that locality and surrounding enviroment are serious, ecotope and human health are caused to long-term huge potential threat.

As the important component part of the ecosystem, microbial population is large, it is wide to distribute, specific surface area is large, breeding is fast, and adaptable to environmental change shows the tolerance of height to radioactive radiation.Utilize biological action to remove, repair and administer radiocontamination and have that selectivity is strong, the treatment time is short, cost is low, do not cause secondary pollution and do not destroy the advantages such as ecotope, become in recent years one of hot research technology of radiocontamination improvement.

Ce 137 (Cs ¹³⁷) be one of important fission product, also be one of byproduct of nuclear fission in nuclear bomb, nuclear weapon test and nuclear reactor, its transformation period is longer, there is higher transport property, easily by biologic chain, shift and cause radiation hazradial bundle, it can discharge gamma rays, in the reparation of environmental radiation contact scar, receives much concern.The transformation period of Ce 137 reaches 30 years, can in environment or the ecosystem, retain, accumulate and move, and causes serious environmental pollution and ecological hazard.If see through feed or breathe, taken in Ce 137, or be subject to being deposited in ground Ce 137 and irradiate, all can there is more lasting impact to health.

Aspect improvement radioactive pollution, traditional method is engineering method, chemical method.Engineering method is the method for physically collecting and isolating radioactive substance, and chemical method is adopt special chemical to fix and remove radiocontamination.Although the application that aforesaid method has been succeeded, as the horse traction woods of Japanese Hiroshima and Liang Ge city, Nagasaki, the republican Bikini Atoll nuclear test site in the Marshall Islands and Australia adds reparation and the reconstruction of nuclear test site, but its cost is very high, be difficult to use in the reparation of big area radiation zone, therefore, engineering and chemical method limitation are very large.Biological restoration is to utilize the method for special biomagnification and immobilization of radioactive pollutent, it is of greatest concern to be mainly that microorganism and plant, particularly microbial function utilize, because of its cost low, be suitable for large area repair, thereby biological restoration radiocontamination becomes the focus of current research.

Research shows: microorganism not only can be by dissolving and precipitation, biological adsorption and absorption, redox etc. are used for administering the radionuclide contamination in environment, and can pass through to change plant rhizosphere microenvironment, thereby improve plant to the absorption of heavy metal ion and radionuclide, volatilization or fixing efficiency.Relevant research has also obtained positive progress, as jana Sitte ^[1]deng studies have shown that the mobility of radioactive nucleus uranium (U) in soil is subject to the impact of microorganism and environmental factor in environment, eva-Maria Burkhardt ^[2]the hyperplasia that research shows Fe (III) reducing bacteria is conducive to comprise that the heavy metal ion of U moved to underground water by soil. c.Hwangwith d.Moreels ^[3]deng research show, bacteria flora can impel soluble state U(VI) to stable U(IV) transform, beyenal ^[4]deng studies show that fixedly U of sulphate reducing bacteria, copplestone ^[5]etc. separated acquisition, can accumulate in a large number ¹³⁷cs, ^238+239+240the microorganism strains of the radionuclides such as Pu, Hu ^[6]deng research show that many microorganisms can adsorb U, as pseudomonas aeruginosa CSU, aspergillus fumigatuswith saccharomyces cerevisiae. entry ^[7]deng research, find that using AM VA Mycorrhizal Fungi can promote row plant pair ¹³⁷cs, ⁹⁰the accumulation of Sr, oneidensisshewanella MR-1, can be effectively by solubility U ⁶⁺, Cr ⁶⁺and Tc ⁷⁺be reduced to insoluble U ⁵⁺, Cr ³⁺and Tc ⁴⁺.

Utilizing microorganism to repair radioactive pollution has had successful example, groudev ^[8]in calendar year 2001, utilize indigenous microorganism to contain the same heavy metal copper of radioelement uranium, radium and thorium, cadmium and lead pollution of soil to Bulgarian south, carry out original position reparation, through the time of 8 months, the level of pollutent dropped to human-body safety scope.

Utilize microorganism to repair radioactive contaminate environment and must consider following two aspects: the first, must be strong radiation-resistant microorganism; The second, during microorganism repairing environment, can not threaten public safety, can not produce secondary pollution.Because most bacterium is more responsive to activity ratio, so the ability of their reparation radiocontamination environment can be subject to larger restriction.Therefore, need to from physical environment, go separation to there is the microorganism of radiation resistance, or by genetic engineering technique, transform some microorganisms and make it to obtain radiation resistance.There is no at present the report of relevant radiation hardness aspergillus and application in radionuclide absorption thereof.

Summary of the invention

For the relevant report there is no at present about radiation hardness aspergillus and application in radionuclide absorption thereof, particularly there is no the application relevant report of radiation hardness aspergillus in caesium biological treatment both at home and abroad.The object of the invention is intended to provide a kind of radiation hardness aspergillus and is adsorbing Cs ¹³⁷application in biological treatment, particularly the invention provides a kind of utilize radiation hardness aspergillus ( aspergillus sp.) application of F77 CGMCC No. 8382 in the biological treatment of absorption caesium.

The main technical schemes that the present invention adopts:

The present invention samples from the Bayangolmongol Autonomous Prefecture local pollution control environment of Xinjiang, take different culture temperature, pH value, substratum is enrichment condition, filter out a collection of well-grown microorganism strains, therefrom optimize the bacterial strain that a strain is numbered F77, molecular water equality campaign checking through the physio-biochemical characteristics of microbial strains, colonial morphology, bacterial classification determines, this bacterial classification be a kind of radiation hardness aspergillus ( aspergillus sp.) F77, through China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preservation, obtaining preserving number is CGMCC No. 8382, utilizes the application in radiocesium biological treatment of this bacterial classification, by this bacterial classification radiation hardness aspergillus of evidence ( aspergillus sp.) F77 CGMCC No. 8382 is for Ni ⁺, Cr ^2+,, Zn ²⁺, Co ²⁺, Pb ²⁺, Hg ²⁺six kinds of ions all have resistance characteristics, wherein to Pb ²⁺, Zn ²⁺ _,ni ⁺tolerance concentration maximum, can reach respectively 1000 mg/L and 500 mg/L, 500 mg/L; To Co ²⁺, Cr ²⁺, Hg ²⁺tolerance take second place, all can reach 200 mg/L; Particularly in radionuclide caesium absorption, application obtains significantly significantly technique effect, thereby has proved that this bacterial classification has good application prospect in the biological treatment of absorption caesium.

The present invention specifically provide a kind of utilize aspergillus ( aspergillus sp.) F77 CGMCC No. 8382.Aspergillus used in the present invention ( aspergillus sp.) separated by Microorgan Application Inst., Xinjiang Agricultural Academy's sampling from the radiocontamination soil of somewhere, Xinjiang Bayangolmongol Autonomous Prefecture area, with different culture temperature, pH value, substratum is enrichment condition, filter out a collection of well-grown microorganism strains, therefrom optimize the bacterial strain that a strain is numbered F77 and before the applying date, be preserved in the international depositary institution of budapest treaty microorganism: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preservation, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101, preservation date is on October 22nd, 2013, preserving number is CGMCC No.8382, this strain culturing temperature 28-30 ℃, 28 ℃ of left and right of the suitableeest culture temperature, this growth is potato 200g, glucose 20g, agar 15g, distilled water IL in PDA media surface, pH nature, through 28 ℃, 48h cultivation, bacterial classification F77 is observed by the microscopy of inserted sheet and hydraulic pressure sheet, this bacterial strain primary hyphae white, be loose fine hair shape, separate, bacterium colony surface rice-pudding leaf green.Sporophore and spore are homochromy, spore subcircular, and bunchiness raw around the sporophore top of expanding, and radially distributes, without mitogenetic stigma.According to above morphological specificity, this bacterial strain of preliminary evaluation is a kind of deuteromycetes Moniliales Moniliaceae list stalk Aspergillus.With reference to < < fungi identification handbook > >, identify, in conjunction with F77 bacterial strain is carried out to morphology, Physiology and biochemistry evaluation, called after radiation hardness aspergillus ( aspergillus sp.).

This bacterial strain aspergillus ( aspergillus sp.) F77 is at PDA substratum, Wort substratum well-grown, through the evidence of Biolog FF identification plate, F77 can utilize tween 80, N-ethanoyl--D-Glucose amine, ribitol, L-arabinose, D-arabitol, arbutin, D-cellobiose, D-wood sugar, oxysuccinic acid, dextrin, erythritol, maltonic acid, a-D-glucose, PEARLITOL 25C,-methyl D-glucoside, 6-O-D-Glucopyranose acyl, D-fructofuranose, D-glucuronic acid, glycerol, glycogen, trisaccharide maltose, quinic acid, Pidolidone, adenosine-5 monophosphate, this salt of a-D-glucose-1-phosphorus, D-ribose, saligenin, D-glucitol, L-sorbose, stachyose, K-trehalose, turanose, Xylitol, y-aminobutyric acid, bromosuccinic acid, FUMARIC ACID TECH GRADE,-hydroxybutyric acid, y-hydroxybutyric acid, P-HPAA, a-ketoglutaric acid, D-malic acid, D-Glucose diacid, sebacic acid, succsinic acid, succsinic acid methyl ester, L-Beta Alanine acid amides, L-Beta Alanine, L-alanyl Padil, altheine, L-Aspartic acid, glycyl-L-glutamic acid, L-phenyl handle propylhomoserin, proline(Pro), Pyrrolidonecarboxylic acid, Serine, the L-amino acid of reviving, 2-monoethanolamine, rotten glycosides, adenosine.

The present invention is by the extraction of total DNA, pcr amplification and the order-checking of ITS1 gene, according to sequencing result, with Blast search software, from the databases such as GenBank, EMBL, recall the ITS1 gene order of the relevant bacterial strain that similarity is higher, with CLUSTAL X, carry out Multiple Sequence Alignment, and adopt Saitou and Nei adjacent method ( neighbor Joining) with MEGA 5.0 softwares, carry out structure and the homology comparison of systematic evolution tree.After measured, radiation hardness aspergillus ( aspergillus sp.) the ITS1 gene order of F77 CGMCC No. 8382 is 600bp.

By the above results and ITS1 DNA homolog analyze, Phylogenetic Analysis result, by the bacterial strain radiation hardness aspergillus of purifying ( aspergillus sp.) F77 CGMCC No. 8382 carries out structure and the diversity analysis of systematic evolution tree, bacterium numbering be F77 with aspergillus flavus strainpT18 homology is the highest, but both also exist obvious difference, shows the bacterial classification of different attribute, the present invention by bacterium numbering be F77 identification of strains be aspergillus ( aspergillus sp.).

Meanwhile, the present invention specifically provide a kind of utilize radiation hardness aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 carries out utilisation technology scheme in radiocesium biological treatment: in the PDA of the stable cesium ion that contains 10mg/L substratum, add 1ml(6772.95 Bake respectively) radiocesium mother liquor, with 2ml(13545.9 Bake) radiocesium mother liquor, inoculation F77, be cultured to the suitableeest growth time, collect sample.The absorption to stability caesium and radiocesium in process of growth by ICP-MS and liquid flashing determining bacterial strain respectively.By test, show, under bacterial classification F77 exists for radiocesium, adsorption rate to stable caesium obviously declines, only have 19.3%, and under one times of radiocesium and the existence of twice radiocesium, little to stability caesium adsorption rate difference, also little for the adsorption rate difference of radiocesium, all reach more than 40%.

The present invention's bacterial strain aspergillus used ( aspergillus sp.) 8382 couples of F77 CGMCC No. are wherein to Pb ²⁺, Zn ²⁺, Ni ⁺tolerance concentration maximum, can reach respectively 1000 mg/L and 500 mg/L, 500 mg/L; To Co ²⁺cr ²⁺, Hg ²⁺tolerance take second place, all can reach 200 mg/L; In radionuclide caesium absorption, application obtains significantly significantly technique effect, thereby has proved that this bacterial classification has good application prospect in the biological treatment of absorption caesium.

The present invention so provide radiation hardness aspergillus ( aspergillus sp.) separation and the cultural method of F77 CGMCC No. 8382.

1. isolation medium adopts: PDA substratum.

2. Isolation and screening condition: take gradient dilution method, take 10g pedotheque in 90mL stroke-physiological saline solution, carry out gradient dilution after 30 ° of C activation 30min, choose 10 ^-2, 10 ^-3, 10 ^-4diluent is coated respectively isolation medium starch PDA culture medium flat plate, and each processes 3 repetitions, puts 30 ° of C and cultivates.The bacterium colony difference streak inoculation that picking shape, size, color etc. are different after growing bacterium colony is in corresponding flat board, until fall without miscellaneous bacteria.

Through cultivate the definite radiation hardness aspergillus of screening ( aspergillus sp.) the loose fine hair shape of F77 CGMCC No. 8382 bacterial strain mycelia on PDA substratum, separate bacterium colony surface rice-pudding leaf green.Can utilize tween 80, N-ethanoyl--D-Glucose amine, ribitol, L-arabinose, D-arabitol, arbutin, D-cellobiose, D-wood sugar, oxysuccinic acid, dextrin, erythritol, maltonic acid, a-D-glucose, PEARLITOL 25C,-methyl D-glucoside, 6-O-D-Glucopyranose acyl, D-fructofuranose, D-glucuronic acid, glycerol, glycogen, trisaccharide maltose, quinic acid, Pidolidone, adenosine-5 ' monophosphate, this salt of a-D-glucose-1-phosphorus, D-ribose, saligenin D-glucitol, L-sorbose, stachyose, K-trehalose, turanose, Xylitol, y-aminobutyric acid, bromosuccinic acid, FUMARIC ACID TECH GRADE,-hydroxybutyric acid, y-hydroxybutyric acid, P-HPAA a-ketoglutaric acid, D-malic acid, D-Glucose diacid, sebacic acid, succsinic acid, succsinic acid methyl ester, L-Beta Alanine acid amides, L-Beta Alanine, L-alanyl Padil, altheine, L-Aspartic acid, glycyl-L-glutamic acid, L-phenyl handle propylhomoserin proline(Pro), Pyrrolidonecarboxylic acid, Serine, the L-amino acid of reviving, 2-monoethanolamine, rotten glycosides, adenosine.

By implementing the concrete technical scheme of the present invention, realize content of the present invention and reach following beneficial effect:

Radiation hardness aspergillus provided by the invention ( aspergillus sp.) F77 CGMCC No. 8382 and the application in cesium ion absorption thereof, Ni ⁺, Cr ²⁺,, Zn ²⁺, Co ²⁺, Pb ²⁺, Hg ²⁺six kinds of ions all have resistance characteristics, wherein to Pb ²⁺, Zn ²⁺, Ni ⁺tolerance concentration maximum, can reach respectively 1000 mg/L and 500 mg/L, 500 mg/L; To Co ²⁺, Cr ²⁺, Hg ²⁺tolerance take second place, all can reach 200 mg/L and can utilize the growth absorption cesium ion of thalline by this law, and radiocesium, also can directly use dry mycelium absorption, maximum adsorption ability can reach 44.5mg/g dry mycelium.

Accompanying drawing explanation

Figure 1 shows that aspergillus ( aspergillus sp.) the colonial morphology hydraulic pressure sheet figure of F77 CGMCC No. 8382.

Figure 2 shows that aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 Phylogenetic dendrograms.

Fig. 3 aspergillus ( aspergillus sp.) the tolerance figure of 8382 pairs of acidity of F77 CGMCC No..

The affect figure of Fig. 4 aspergillus (Aspergillus sp.) F77 CGMCC No. 8382 adsorption times on caesium absorption behavior.

Fig. 5 aspergillus ( aspergillus sp.) the affect figure of F77 CGMCC No. 8382 caesium starting point concentrations on adsorption effect.

Fig. 6 aspergillus (Aspergillus sp.) F77 CGMCC No. 8382 adsorption isotherm matched curve figure.

Fig. 7 potassium to aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 growth and absorption caesium affect figure.

Fig. 8 aspergillus ( aspergillus sp.) the affect figure of F77 CGMCC No. 8382pH on adsorption effect.

The different biomasses of Fig. 9 to aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 absorption cesium ions affect figure.

Figure 10 adsorption time for to aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 absorption cesium ions affect figure.

Figure 11 temperature to aspergillus ( aspergillus sp.) the affect figure of F77 CGMCC No. 8382 thalline on cesium ion absorption.

Figure 12 aspergillus ( aspergillus sp.) the adsorption rate figure of 8382 pairs of stable caesiums of F77 CGMCC No. and radiocesium.

Embodiment

Below in conjunction with specific embodiment, further illustrate the present invention, certainly, these embodiment are only for the present invention is described, and are not used in restriction the scope of protection of present invention.

The main raw and auxiliary material, reagent and the plant and instrument that in the present invention, relate to:

Substratum is selected: PDA media surface is potato 200g, glucose 20g, agar 15g, distilled water IL, pH nature.

Key instrument and reagent: MSSPX-250 type biochemical cultivation case, MLS-3020 high-pressure steam sterilizing pan, the single two-sided clean work station of SW-CJ-1F Type B, E360K whizzer, constant-temperature table HWY-100.PCR instrument Eppendorf No:5345, electrophoresis apparatus Bio-Rad Mode 200/2.0, gel imaging instrument United-Bio, GK-330C plus, PCR premixed liquid (TaKaRa Biotechnology), all the other reagent are analytical pure.Ultrasonic disruption Yi Wei U.S. Sonics, VC 130.Xseries II type inductivity coupled plasma mass spectrometry (ICP-MS), U.S. THERMO company; The accurate pH meter of Seven Easy Plus S20P type, Shanghai plum Teller-Tuo benefit Instrument Ltd.; DHG-924OA type electric heating constant-temperature blowing drying box, Shanghai Yiheng Scientific Instruments Co., Ltd; THZ-82 gas bath constant temperature oscillation case, Community of Jin Tan County Cheng Hui instrument plant; GMSX-280 pressure steam sterilizer, Beijing is bright Medical Instruments company limited forever.Reagent is analytical pure, and water is ultrapure water, 18.2 M Ω cm.

All reagent of selecting in the present invention and instrument are all well known selecting, but do not limit enforcement of the present invention, and other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention.

embodiment mono-: aspergillus ( aspergillus sp.) separation, the cultivation of F77CGMCC No. 8382

1. separation: radiation hardness aspergillus used in the present invention ( aspergillus sp.) the sampling separation from the radiocontamination soil of somewhere, Xinjiang Bayangolmongol Autonomous Prefecture area of F77You Microorgan Application Inst., Xinjiang Agricultural Academy, utilize traditional plating method to isolate the microorganism in soil layer, plate streak purifying bacterial strain, take different culture temperature, pH value, substratum is enrichment condition, filter out a collection of well-grown microorganism strains, therefrom optimize the bacterial strain that a strain is numbered F77.

Separating step: according to gradient dilution method, take 10g pedotheque in 90mL stroke-physiological saline solution, 30 ° of C carry out gradient dilution after constant temperature oscillation case activation 30min, choose 10 ^-2, 10 ^-3, 10 ^-4diluent is coated respectively the flat board of PDA substratum, and each processes 3 repetitions, puts 30 ° of C and cultivates.The bacterium colony difference streak inoculation that picking shape, size, color etc. are different after growing bacterium colony is in new isolation medium PDA substratum, until fall without miscellaneous bacteria.A bacterial strain part after purifying is adopted to the mode preservations such as freeze-drying ampoul tube, glycerine pipe and liquid nitrogen, and a part is stored in 4 ° of C and is directly used in follow-up study.

2. culture condition: the inoculation of purifying, to solid potato culture medium inclined-plane, is cultivated 6 days in 30 ℃, put into 4 ℃ of refrigerators and save backup.

Concrete: this strain culturing temperature 28-30 ℃, 30 ℃ of left and right of the suitableeest culture temperature; This growth is potato 200g, glucose 20g, agar 15g, distilled water IL in PDA media surface, and pH nature, through 30 ℃, 48h cultivation.

Radiation hardness aspergillus used in the present invention ( aspergillus sp.) F77 bacterial strain has been preserved in the international depositary institution of budapest treaty microorganism: China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preservation, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode: 100101, preservation date is on October 22nd, 2013, culture presevation number is CGMCC No.8382, this strain culturing temperature 28-30 ℃, 30 ℃ of left and right of the suitableeest culture temperature; This growth is potato 200g, glucose 20g, agar 15g, distilled water IL in PDA media surface, and pH nature, through 30 ℃, 48h cultivation.Bacterial classification F77 observes by the microscopy of inserted sheet and hydraulic pressure sheet, this bacterial strain primary hyphae white, loose fine hair shape.Sorus rice-pudding leaf green, spore subcircular, bunchiness raw in the sporophore top surrounding of expanding, and becomes radial distribution, without mitogenetic stigma.Referring to accompanying drawing 1.According to above morphological specificity, this bacterial strain of preliminary evaluation is a kind of deuteromycetes Moniliales Moniliaceae list stalk Aspergillus.With reference to < < fungi identification handbook > >, identify, in conjunction with F77 bacterial strain is carried out to morphology, Physiology and biochemistry evaluation, called after aspergillus ( aspergillus sp.).

This bacterial strain aspergillus ( aspergillus sp.) F77 is at PDA substratum, Wort substratum well-grown, through the evidence of Biolog FF identification plate, F77 can utilize tween 80, N-ethanoyl--D-Glucose amine, ribitol, L-arabinose, D-arabitol, arbutin, D-cellobiose, D-wood sugar, oxysuccinic acid, dextrin, erythritol, maltonic acid, a-D-glucose, PEARLITOL 25C,-methyl D-glucoside, 6-O-D-Glucopyranose acyl, D-fructofuranose, D-glucuronic acid, glycerol, glycogen, trisaccharide maltose, quinic acid, Pidolidone, adenosine-5 ˊ monophosphate, this salt of a-D-glucose-1-phosphorus, D-ribose, saligenin, D-glucitol, L-sorbose, stachyose, K-trehalose, turanose, Xylitol, y-aminobutyric acid, bromosuccinic acid, FUMARIC ACID TECH GRADE,-hydroxybutyric acid, y-hydroxybutyric acid, P-HPAA, a-ketoglutaric acid, D-malic acid, D-Glucose diacid, sebacic acid, succsinic acid, succsinic acid methyl ester, L-Beta Alanine acid amides, L-Beta Alanine, L-alanyl Padil, altheine, L-Aspartic acid, glycyl-L-glutamic acid, L-phenyl handle propylhomoserin, proline(Pro), Pyrrolidonecarboxylic acid, Serine, the L-amino acid of reviving, 2-monoethanolamine, rotten glycosides, adenosine.The results are shown in Table 1.

Table 1: the impact of the factors such as temperature, pH on bacterial strain F77 growth

Temperature (℃)	4	15	25	30	32	35
							Growing state	-	+	+	++++	+++	+++
Temperature (℃)	38	45	50
							Growing state	++	-	-
pH	1	2	3	4	5	6
							Growing state	-	-	+	+	+	+++
pH	7	8	9	10
							Growing state	+++	+	-	-
NaCl concentration	0％	1％	2％	3％	4％	5％
							Growing state	+	++	+++	+++	+++	+
NaCl concentration	6％	7％	8％	9％	10％
							Growing state	-	-	-	-	-
Penbritin μ g/ml	50	60	70	80	90	100
							Growing state	-	-	-	-	-	-

3. bacterial classification is described: radiation hardness aspergillus of the present invention ( aspergillus sp.) F77 CGMCC No. 8382 is after PDA culture medium culturing 5d, microscopy by inserted sheet and hydraulic pressure sheet is observed, this bacterial strain mycelia fine hair shape that loosens, separate, bacterium colony surface is rice-pudding leaf green, and sporophore and spore are homochromy, spore subcircular, bunchiness raw around the sporophore top of expanding, and radially distributes, without mitogenetic stigma.Radiation hardness aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 colonial morphology figure are referring to accompanying drawing 1.

embodiment bis-: aspergillus ( aspergillus sp.) F77CGMCC No. 8382 molecular levels evaluations

1. DNA extraction: fungi extracting method is as follows:

(1) 200 mg thalline, liquid nitrogen grinding, adds 3 ml 3% CTAB Extraction buffers; 65 ℃ of water-bath 45 min, 4 ℃ of 4000 centrifugal 20 min of r/min.

(2) get supernatant and forward in centrifuge tube, add 4 μ l 10 mg/ml proteolytic enzyme, 37 ℃, water-bath 1 h.

(3) add the saturated phenol of 800 μ l Tris, shake up the centrifugal 10min of 13000 r/min; Get supernatant.

(4) add isopyknic chloroform/primary isoamyl alcohol, shake up, centrifugal 10 min of 13000 r/min; Get supernatant.

(5) add the RNA enzyme of 10 mg/ml, 37 ℃ of water-baths processing of spending the night.

(6) add 800 μ l chloroform/primary isoamyl alcohol, shake up, centrifugal 10 min of 13000 r/min; Get supernatant.

(7) add 600 μ l primary isoamyl alcohol ,-20 ℃ of precipitation 30 min, collecting precipitation, 75% alcohol, rinses super clean bench vacuum-drying.

(8) 100 μ l TE dissolving DNAs ,-20 ℃ save backup.The RNA enzyme that adds 1 μ l, 37 ℃ of water-bath 1 h; Add 400 μ l chloroform/primary isoamyl alcohol (24: 1), centrifugal 10 min of 12000 r/min, repeat 2 times.

2. the gene amplification of ITS1 gene and order-checking, with fungi ITS1 gene gene universal primer, increase:

Primer I TS11:5'-TCCGTAGGTGAACCTGCGG-3'

ITS14： 5'-TCCTCCGCTTATTGATATGC-3'

PCR amplification reaction system is 50 μ L, and reaction conditions is: 95 ° of C, 5 min; 95 ° of C, 45 S, 57 ° of C, 30 S, 72 ° of C, 90S, 30Cycles; 72 ° of C, 7min.Amplified production (approximately 600 bp), pcr amplification product detects with 1% agarose gel electrophoresis, amplified production is checked order, the ITS1 gene order of bacterial strain F77 is measured, after measured, aspergillus (Aspergillus sp.) F77 CGMCC No. 8382 ITS1 gene orders are 600bp, referring to the attached gene order table SEQUENCE LISTING providing.

3. ITS1 sequence alignment and Phylogenetic Analysis

The present invention is by the extraction of total DNA, pcr amplification and the order-checking of ITS1 gene gene.According to sequencing result, with Blast search software, from the databases such as GenBank, EMBL, recall the ITS1 gene gene order of the relevant actinomycetes strain that similarity is higher, with CLUSTAL X, carry out Multiple Sequence Alignment, and adopt Saitou and Nei adjacent method ( neighbor Joining) with MEGA 5.0 softwares, carry out the structure of systematic evolution tree.Result, referring to shown in accompanying drawing 2, can find out from dendrogram, and this bacterial strain and Aspergillus flavus strain PT18, in same branch, show that the sibship of the two is nearest, and comparison result shows, F77 and Aspergillus flavus ( aspergillus flavuspT18) homology is the highest, according to microorganism classification method, by bacterium numbering, is that F77 bacterial strain is initially identified as aspergillus (Aspergillus sp.).

Based on ITS1 gene order amplification and the order-checking of PCR product, acquisition 600bp sequence, through GenBank Blast homologous sequence compare of analysis, the flavus that itself and prior art are reported (Aspergillus) homology is higher; From GenBank, obtain reference culture ITS1 gene order, carry out homology evolutionary analysis, constructing system evolutionary tree, result is referring to accompanying drawing 2; Result shows, F77 bacterial strain is under the jurisdiction of Aspergillus, with Aspergillus flavus ( aspergillus flavuspT18) close source relation is nearest, but both also exist obvious difference, shows the bacterial classification of different attribute, determines that bacterial strain F77 is radiation hardness Aspergillus strain, called after aspergillus of the present invention ( aspergillus sp.).

embodiment tri-: aspergillus ( aspergillus sp.) capability of resistance to radiation of F77CGMCC No. 8382

Microbial treatment environmental radiation contact scar need meet two conditions, in radiation environment, can survive and have higher adsorption selectivity to polluting nucleic.Fungi aspergillus F77 extracts in Soil Contaminated with Radionuclides, after slant culture, and warp ⁶⁰co radioactive source gamma-ray irradiation, irradiation dose is accumulated as 10 kGy.By irradiation sample and non-irradiation sample, cultivate comparison, find the not irradiated impact of its growth, aspergillus F77 has very strong capability of resistance to radiation.The radiation characteristic of F77 (gamma-radiation) is with Ferreira etc. ^[9]the method of having set up is verified.Bacterium is cultured to stationary phase at PDA liquid nutrient medium, 4 ℃ centrifugal after with after physiological saline washing, with the concentration that suspends in physiological saline, be controlled at 1 * 10 ⁷– 10 ⁸c.f.u/ ml, is divided into every part of 2ml, ⁶⁰under Co, with the dose rate of 0.167 kGy/ min, at room temperature irradiate.Irradiation dose is raised to 10.0 kGy with the amplitude of 2.0 kGy from 0Gy.The sample irradiating is applied on PDA flat board and after 3-5 days, sees if there is survival 30 ℃ of cultivations after dilution, to detect F77 for gamma-emitting resistance characteristics.Test show aspergillus provided by the invention ( aspergillus sp.) F77 CGMCC No. 8382 irradiation of the irradiation doses through 10 kGy energy normal growths, there is stronger radiation resistance.

embodiment tetra-: aspergillus ( aspergillus sp.) 8382 pairs of sour tolerances of F77CGMCC No.

The vital movement of microorganism, substance metabolism and acid have substantial connection, and different microorganisms process of growth differs to the requirement of environment.Growing environment determines the biomass of microorganism, thereby affects its absorption to metal ion.In acidic solution, metallic element exists mainly with ionic species, is beneficial to it by biological adsorption and absorption.For this reason, investigated aspergillus F77 to sour tolerance.In temperature, be that 30 ℃, incubation time are under 70 h conditions, aspergillus F77 biomass (dry mycelium quality) can be referring in accompanying drawing 3 with the relation of culture medium solution pH.

From accompanying drawing 3, aspergillus F77 biomass increases with the increase of culture medium solution pH, and during pH<2.5, dry mycoplasma amount all, below 0.6 g, illustrates that acid has suppressed the growth of aspergillus F77.During pH 4.5, biomass reaches maximum value, and corresponding dry mycoplasma amount is 1.02 g.After pH>4.5, biomass no longer increases.Some investigators think, the vital movement of microorganism, substance metabolism and acid have substantial connection, and appropriate acid is beneficial to microbial growth, and too high or too low acid is disadvantageous to microorganism growth.

Be mainly manifested in: the too much hydrogen ion in (1) solution may change the charge property on microorganism surface, affect its absorption to nutritive substance; (2) acid affects microorganism cells except direct, also can affect the ionization of Cucumber in substratum, thereby remote effect microorganism, (3) enzyme its maximum activity of competence exertion under most suitable acidity only, the variation of acidity can make the activity decreased of enzyme, and then affects the Biochemical processes in microorganism cells.

As can be seen here, the biomass of aspergillus F77 is along with pH value increases and increases.During pH<2.5, the growth of aspergillus F77 is obviously suppressed; During pH>4.5, its hydrogen ion reduces microbial growth is not almost had to what impact; When pH=4.5, the quality of dry bacterium reaches maximum value 1.02 g.

embodiment five: aspergillus ( aspergillus sp.) the kinetics of adsorption research of 8382 pairs of stable cesium ions of F77CGMCC No.

Aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 adsorption experiment methods

Aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 adsorption experiments take following process: pipette 100 mL PDA culture medium solutions in 500 mL Erlenmeyer flasks, according to experiment, need to quantitatively add cesium ion solution, inoculate appropriate F77 bacterial classification suspension liquid (20 mL sterilized waters evenly mix with the bacterial classification on inclined-plane), 180 rpm, 30 ℃ of concussions are cultured to experiment required time.Solution centrifugal after cultivating, supernatant liquor is measured cesium ion concentration with ICP-MS, and gained thalline dries to constant weight under 60 ℃ of conditions, for calculating biomass.

The method of calculation of unit adsorptive capacity and adsorption rate:

By unit adsorptive capacity (qe) and adsorption rate (R), characterize the absorption situation of microorganism to caesium, method of calculation are shown in formula (1), (2).

q _e=(C ₀-C _t)×V÷m (1)

R=（C ₀-C _t）÷C ₀×100% (2)

Wherein, C0 is the starting point concentration of caesium in solution, mg/L; Ct is absorption t concentration of caesium in solution after the time, mg/L; V is adsorbent solution volume, L; M is the dry mycoplasma amount of aspergillus F77, g.

Aspergillus ( aspergillus sp.) F77 CGMCC No. 8382 kinetics of adsorption researchs

At adsorption temp, be 30 ℃, pH 6.5, cesium ion concentration is that under the condition of 10 mg/L, adsorption rate is rapid growth along with the time, in 70 h left and right, reaches adsorption equilibrium, and adsorption rate is in 70% left and right, and after this adsorption rate temporal evolution is not quite referring to accompanying drawing 4.This is mainly because along with microorganism, to cesium ion, absorption increases, cell surface reaches capacity gradually to the absorption of cesium ion, on cell walls, the repulsion of its generation is strengthened, the resistance that causes metal ion further to enter into cell surface increases, and then reaches the relative equilibrium stage.Along with the prolongation of incubation time, microorganism slightly reduces the adsorption rate of caesium, shows that thalline starts death along with nutritive substance approach exhaustion, even occurs autolysis, breaks solid-liquid adsorption equilibrium, causes thalline adsorption rate to decline.

Adopt false second-order kinetic equation: the kinetics of adsorption of simulation aspergillus F77, is expressed as follows:

t÷q _t=1÷(kq ² _e)+1÷q _e×t (5)

Wherein: t is duration of oscillation, h; Qt is the t adsorptive capacity of aspergillus F77 to caesium constantly, mg/g; K is false second order rate constant, g ﹒ mg ^-1﹒ h ^-1; The adsorptive capacity of aspergillus F77 to caesium when qe is balance, mg/g.With t/qe, to the t straight line of mapping to obtain, shown in the medium and small figure of accompanying drawing 4, by slope and the intercept of straight line, try to achieve k=0.091 g ﹒ mg ^-1﹒ h ^-1, qe=0.943 mg/g, coefficient R 2=0.991, illustrates that aspergillus F77 meets false second-order kinetics model to the absorption of caesium.。

Visible, aspergillus F77 growth rate is very fast, stops growing and cesium ion is adsorbed and reaches balance after 70 h, and after approximately 200 h, autolysis appears in microorganism, causes caesium adsorption rate to decline.

embodiment six: cesium ion starting point concentration to aspergillus ( aspergillus sp.) impact of F77 CGMCC No. 8382 absorption

At adsorption temp, be that 30 ℃, pH 6.5, incubation time are under the condition of 70 h, aspergillus F77 is inoculated in containing in different concns cesium ion (0.1,1.0,10.0,100,200,500 mg/L) nutrient solution, adsorptive capacity with cesium ion change in concentration referring to shown in accompanying drawing 5.

Rising along with cesium ion concentration, aspergillus F77 also increases gradually to the adsorptive capacity of caesium, in tested cesium ion concentration range, unit adsorptive capacity and the positive correlation of cesium ion concentration, when caesium concentration is 500 mg/L, aspergillus F77 is 30 mg/g to the unit adsorptive capacity of caesium, but does not reach maximal absorptive capacity, illustrates that aspergillus F77 has stronger adsorption to cesium ion.Thalline is small, and its specific surface area is large, increases the concentration that GOLD FROM PLATING SOLUTION belongs to ion, has increased the probability of collision between metal ion and thalline, contributes to the absorption of thalline to caesium.In addition,, along with GOLD FROM PLATING SOLUTION belongs to the increase of ionic concn, the resistance to mass transfer between solid-liquid two-phase is overcome, and has improved the probability of collision between metal ion and thalline, is beneficial to the absorption of thalline to metal ion.

In experiment cesium ion concentration range, aspergillus F77 all more than 60%, shows that aspergillus F77 has stronger adsorptive power to caesium to the adsorption rate of caesium.In most cases, microorganism meets Freundlich isothermal adsorption formula to the adsorption process of radionuclide, with Freundlich equation, experimental data is carried out to matching:

Q _e=K _FC _e ^1/n (3)

Take the logarithm in both sides:

1gq _e=1gK _F+1÷n×1gC _e (4)

Wherein, KF is Freundlich adsorption index; Qe is equilibrium adsorption capacity, mg/g; Ce is adsorption equilibrium mass concentration, mg/L.Lgqe is mapped to lgCe, be shown in accompanying drawing 6, obtain the straight line that coefficient R 2 is 0.998, illustrate that aspergillus F77 can describe with Freundlich isothermal adsorpting equation well to the adsorption process of caesium, calculates KF=0.25 by intercept.

Visible, aspergillus F77 has higher adsorption rate to caesium, within the scope of research caesium starting point concentration, aspergillus F77 is greater than 60% to the adsorption rate of caesium, adsorptive capacity reaches 30 mg/g when caesium concentration is 500 mg/L, and its absorption behavior more meets Freundlich equation, its KF=0.25.

embodiment seven: potassium ion to aspergillus ( aspergillus sp.) impact of F77 CGMCC No. 8382 absorption

In surrounding medium, coexisting ion is to affect microorganism to one of important factor of object ion absorption.Potassium is extensively present in various surrounding mediums, is also the essential element of microorganism growth simultaneously, and microbial growth is had to considerable influence.Because potassium and caesium belong to alkali metal together, there is similar physico-chemical property, between potassium and caesium, certainly exist like this absorption and absorb competitive relation.At adsorption temp, be that 30 ℃, pH 6.5, cesium ion concentration are that 10 mg/L, incubation time are under the condition of 70 h, investigated the impact of different concns potassium (5 mg/L ~ 20000 mg/L) on aspergillus F77 growth and absorption caesium in solution, result is referring to accompanying drawing 7.

By referring to shown in accompanying drawing 7, along with the increase of potassium concentration in solution, the biomass of aspergillus F77 also constantly increases, and when the concentration of potassium is 10000 mg/L, biomass (dry weight) reaches the highest 1.411 g.Along with the increase of potassium concn, the adsorptive capacity of caesium obviously declines.Potassium concn in solution is greater than after 5000 mg/L, and the specific absorption of caesium is only 15% left and right, but no longer reduces.This is to reach balance because of the competition exchange along with caesium and potassium, and the specific absorption of caesium tends towards stability.

Visible, potassium ion is conducive to the growth of aspergillus F77, and when the concentration of potassium ion is 10000 mg/L, biomass (dry weight) reaches the highest 1.411 g.But when potassium concentration surpasses approximately 30 times of caesium concentration, aspergillus F77 obviously reduces the adsorption rate of caesium.When potassium concentration is greater than 5000 mg/L, aspergillus F77 only has 15% left and right to the adsorption rate of caesium.Referring to accompanying drawing 7

embodiment eight: affect aspergillus ( aspergillus sp.) F77CGMCC No. 8382 adsorpting factor researchs

The substratum adopting: PDA substratum: potato 200g, glucose 20g, distilled water 1000ml, pH nature.Make Cs ⁺concentration is 20mg/L PDA substratum respectively, and leaves and takes a part of initial medium that does not connect bacterial classification and oppose in the same old way, to record Cs in substratum ⁺actual value.

From inclined-plane, picking thalline is made bacteria suspension, toward the above-mentioned different concns Cs that contains ⁺pDA substratum in inoculation experiments bacterial strain spore suspension respectively, 180rpm, culture temperature is 30 ℃, shaking table is cultivated after 72h, No. 1 filter paper filtering nutrient solution, separating thallus and filtrate, through inductivity coupled plasma mass spectrometry (ICP-MS) test sample, calculate bacterial strain absorption Cs respectively ⁺ability.Affect adsorpting factor research trial as follows:

The impact of 1.pH on absorption: solution acidity is one of principal element affecting aspergillus F77 absorption.In system, a large amount of hydrogen ions that exist, change microorganism surface properties, the absorption of impact to metal ion.For this reason, investigated the impact of system acidity on aspergillus F77 absorption caesium.In temperature, be that 30 ℃, caesium concentration are that 10 mg/L, incubation time are under the condition of 70 h, in different pH culture medium solutions, aspergillus F77 can be referring in accompanying drawing 8 to the absorption situation of caesium.

Aspergillus F77 is proportionate to the absorption of caesium and pH, and adsorption rate raises with pH value of solution and promptly increases.When 2<pH<3.5, caesium is adsorbed hardly.It is generally acknowledged, between hydrogen ion and metallic cation, have competitive adsorption effect ^[10].Hydrogen ion in solution has occupied the functional adsorption group (as hydroxyl, amino, carboxyl etc.) on aspergillus F77 surface, affects the absorption of microorganism to caesium.During pH>3.5, the adsorption rate of caesium increases rapidly, and this is consistent with aspergillus F77 growth tendency, illustrates along with strain growth measures increase, and aspergillus F77 surface adsorption site sharply increases, and has increased the interaction of caesium and adsorption group.And after pH>5, adsorption rate approximately 70% rear growth tends towards stability.Explanation is along with pH value of solution raises, and the adsorption site on aspergillus F77 surface is saturated gradually, thereby adsorption rate no longer increases.For obtaining best adsorption effect, follow-up adsorption experiment all carries out in more than 5 solution at pH.

As can be seen here, hydrogen ion is the important adsorption inhibitor of cesium ion, and when 2.0<pH<3.5, caesium is not almost adsorbed, in the slightly acidic system of pH>5.0, aspergillus F77 reaches 70% to the adsorption rate of caesium.

2. biomass is for the impact of absorption: in order to determine that the bacterial strain filter out is the thalline absorption after the absorption of growth limit, limit or growth to the absorption of cesium ion, done thalline adsorption experiment, by PDA substratum, cultivated in a large number collection thalline.First carried out the impact of thalline quality for absorption.

Take respectively the thalline (0.1g of different mass, 0.2 g, 0.5 g, 0.8 g, 1 g, 1.2 g, 1.5 g) be placed in respectively in the solution system that 15ml cesium ion concentration is 20mg/l, absorption is spent the night, sampling is measured through ICP-MS, result shows: the adsorption rate of thalline, be all less than growth adsorption rate, and determined that the bacterial strain filtering out is the absorption of growth limit, limit to the suction type of the absorption of caesium.Along with biomass increases, adsorption rate also slowly increases, and when reaching a certain amount of, adsorption rate no longer includes variation, reaches balance.Along with the increase of biomass, under fixing metal ions CONCENTRATION STATE, the adsorptive capacity of unit thalline reduces.Referring to accompanying drawing 9.

3. the impact that adsorption time adsorbs for thalline: take 0.5g thalline, concussion is suspended in the 10ml solution system that cesium ion concentration is 20mg/l respectively, interval 1h sampling.Sample is measured through ICP-MS, and result shows that thalline adsorption rate is well below growth absorption, along with the not significantly increase of increase adsorption rate of time, just in certain value scope, fluctuates up and down, and thalline absorption is likely a transients, referring to accompanying drawing 10.

4. the impact that temperature is adsorbed for thalline: take 0.5g thalline, concussion is suspended in the 10ml solution system that caesium (strontium) ionic concn is 20mg/l, is placed in respectively 10 ℃ of-90 ℃ of water-baths, absorption 1h sampling.Sample is measured through ICP-MS.

For F77 bacterial strain, along with the rising adsorption rate of temperature declines on the contrary, prove that temperature has impact significantly for F77 thalline absorption cesium ion.Referring to accompanying drawing 11.

embodiment nine: aspergillus ( aspergillus sp.) the resistance to heavy metal characteristic of F77CGMCC No. 8382

By bacterial strain aspergillus of the present invention ( aspergillus sp.) F77 CGMCC No. 8382 is inoculated in the seed test tube that 5mlPDA liquid nutrient medium is housed, and in 30 ℃ of cultivations, after 200rpm shaking culture 36h, is inoculated in the Ni that contains respectively different concns by 2% inoculum size ⁺, Cr ²⁺, Zn ²⁺, Co ²⁺, Pb ²⁺, Hg ²⁺in PDA liquid fermenting bottle, the amount of adding is the bottled 100ml PDA of 500ml triangle liquid nutrient medium, in 30 ℃ of cultivations, and 220rpm shaking culture 72h, the growing state of observation bacterial classification; Can draw bacterial strain aspergillus ( aspergillus sp.) 8382 couples of Ni of F77 CGMCC No. ⁺, Cr ²⁺,, Zn ²⁺, Co ²⁺, Pb ²⁺, Hg ²⁺six kinds of ions all have resistance characteristics, wherein to Pb ²⁺, Zn ²⁺, Ni ⁺tolerance concentration maximum, can reach respectively 1000 mg/L and 500 mg/L, 500 mg/L; To Co ²⁺, Cr ²⁺, Hg ²⁺tolerance take second place, all can reach 200 mg/L; PDA substratum (potato 200g, glucose 20g, distilled water 1000ml, pH nature).Through 28 ℃, 48h cultivation.，

embodiment ten: aspergillus ( aspergillus sp.) 8382 couples of Wen of F77CGMCC No. Ding Cesium ion and radiocesium adsorption test

Whether variant for the absorption of radiocesium and stability caesium for measuring F77, designed following experimental program, in the PDA of the stable cesium ion that contains 10mg/L substratum, add 1ml(6772.95 Bake respectively) radiocesium mother liquor, with 2ml(13545.9 Bake) radiocesium mother liquor, inoculation F77, be cultured to the suitableeest growth time, collect sample.The absorption to stability caesium and radiocesium in process of growth by ICP-MS and liquid flashing determining bacterial strain respectively.

Table 2: bacterial strain designs radiocesium-growth adsorption test

Shown in accompanying drawing 12, bacterial classification F77, under radiocesium exists, obviously declines to the adsorption rate of stable caesium, and under one times of radiocesium and the existence of twice radiocesium, little to stability caesium adsorption rate difference, also little for the adsorption rate difference of radiocesium.

In sum, by above-mentioned serial experiment, verify, by the present invention, utilize radiation hardnessaspergillus ( aspergillus sp.) the growth absorption cesium ion of F77 CGMCC NO.8382 thalline, and radiocesium, also can directly use dry mycelium absorption, maximum adsorption ability can reach 44.5mg/g dry mycelium.

Reference:

(1) Sitte, J., et al., Microbial links between sulfate reduction and metal retention in uranium- and heavy metal-contaminated soil. Appl Environ Microbiol, 2010. 76(10): 3143-3152。

(2) Burkhardt, E.M., et al., Impact of biostimulated redox processes on metal dynamics in an iron-rich creek soil of a former uranium mining area. Environ Sci Technol, 2010. 44(1): 177-183。

(3) Beyenal, H., et al., Uranium immobilization by sulfate-reducing biofilms. Environ Sci Technol, 2004. 38(7):. 2067-2074。

(4) Hwang, C., et al., Bacterial community succession during in situ uranium bioremediation: spatial similarities along controlled flow paths. ISME J, 2009. 3(1): 47-64。

(5) Bhainsa, K. C. and S. F. D'Souza (1999). "Biosorption of uranium (VI) by Aspergillus fumigatus." Biotechnology techniques 13(10): 695-699。

(6) the yellow people's livelihood, Zheng Leping, Zhu Jinliang. microorganism is to the enrichment of uranium in water and reduction. nuclear technique. 2002,25 (2): 123-131.

(7) Entry, J., L. Watrud, et al. (1999). "Accumulation of 137Cs and 90Sr from contaminated soil by three grass species inoculated with mycorrhizal fungi." Environmental Pollution 104: 449-457。

(8) Groudev, S., P. Georgiev, et al. (2001). "Bioremediation of a soil contaminated with radioactive elements." Hydrometallurgy 59(2-3): 311-318。

（9） Ferreira, A. C., Nobre, M. F., Rainey, F. A., Silva, M. T., Wait, R., Burghardt, J., Chung, A. P. & Da Costa, M. S. (1997) 。

（10） Harshala Parab. Shreeram Joshi etal. Uranium removal from aqueous solution by coir pith: equilibrium and kinetic studies[J]. Bioresource Technology. 2005, 96(11):1241-1248。

SEQUENCE LISTING

<110> Microorgan Application Inst., Xinjiang Agricultural Academy

<120> radiation hardness aspergillus and the application in the biological treatment of absorption Ce 137 thereof

<130> radiation hardness aspergillus and the application in the biological treatment of absorption Ce 137 thereof

<160> 1

<170> PatentIn version 3.3

<210> 1

<211> 600

<212> DNA

<213> Aspergillus sp.F77 CGMCC No. 8382

<220>

<221> ITS1

<222> (1)..(600)

<400> 1

cttcccgtaa agggtacctg cggaaggatc attaccgagt gtagggttcc tagcgagccc 60

aacctcccac ccgtgtttac tgtaccttag ttgcttcggc gggcccgcca ttcgtggccg 120

ccgggggctc tcagccccgg gcccgcgccc gccggagaca ccacgaactc tgtctgatct 180

agtgaagtct gagttgattg tatcgcaatc agttaaaact ttcaacaatg gatctcttgg 240

ttccggcatc gatgaagaac gcagcgaaat gcgataacta gtgtgaattg cagaattccg 300

tgaatcatcg agtctttgaa cgcacattgc gccccctggt attccggggg gcatgcctgt 360

ccgagcgtca ttgctgccca tcaagcacgg cttgtgtgtt gggtcgtcgt cccctctccg 420

ggggggacgg gccccaaagg cagcggcggc accgcgtccg atcctcgagc gtatggggct 480

ttgtcacccg ctctgtaggc ccggccggcg cttgccgaac gcaaatcaat cttttccagg 540

ttgacctcgg atcaggtagg gatacccgct gaacttaagc atatcaaaag acggaggaaa 600

Claims (3)

1. An aspergillus ( aspergillus sp.) F77, it is characterized in that, described aspergillus ( aspergillus sp.) F77 deposit number is CGMCC No. 8382.
2. Aspergillus as claimed in claim 1 ( aspergillus sp.) F77 is in application in radionuclide absorption.
3. One kind utilize radiation hardness aspergillus ( aspergillus sp.) application of F77 CGMCC No. 8382 in the biological treatment of absorption caesium.