CN104087287A - Water-soluble polymer pH fluorescent probe PRAM and preparation method thereof - Google Patents
Water-soluble polymer pH fluorescent probe PRAM and preparation method thereof Download PDFInfo
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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- C09B69/10—Polymeric dyes; Reaction products of dyes with monomers or with macromolecular compounds
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- C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
- C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
- C08F220/52—Amides or imides
- C08F220/54—Amides, e.g. N,N-dimethylacrylamide or N-isopropylacrylamide
- C08F220/56—Acrylamide; Methacrylamide
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- C09B11/04—Diaryl- or thriarylmethane dyes derived from triarylmethanes, i.e. central C-atom is substituted by amino, cyano, alkyl
- C09B11/10—Amino derivatives of triarylmethanes
- C09B11/24—Phthaleins containing amino groups ; Phthalanes; Fluoranes; Phthalides; Rhodamine dyes; Phthaleins having heterocyclic aryl rings; Lactone or lactame forms of triarylmethane dyes
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Abstract
The invention discloses a water-soluble polymer pH fluorescent probe PRAM and a preparation method thereof. A structural formula of the water-soluble polymer pH fluorescent probe PRAM is shown in the description. The pH fluorescent probe PRAM has high applicability to a solvent and good anti-interference property, and is very sensitive to H<+>. The preparation method of the pH fluorescent probe PRAM is simple, and is fast in reaction rate and small in energy consumption.
Description
Technical field
The present invention relates to fluorescent probe technique field, relate in particular to a kind of water-soluble polymer pH fluorescent probe PRAM and preparation method thereof.
Background technology
PH fluorescent probe can be used for detection and the research work in the fields such as biology, chemistry, environment, food.More for pH fluorescent probe relevant report at present, but most practicality that lack, wherein the problem of most critical is that solvent suitability is inadequate, and most application need to be carried out in 100% the aqueous solution, and most of pH fluorescent probe need to carry out work in the mixed solvent of organic solvent and water.Secondly, freedom from jamming is inadequate, and existing pH fluorescent probe is under certain condition to H
+have outside selective response, toward contact, some metal ions are had to certain weak reponse, can cause like this interference.These defects have caused the limitation of pH fluorescent probe in application aspect.
High molecular fluorescent probe often has small molecules fluorescent probe and does not possess a little, as regulatable solvability, can multifunction, there is EPR effect etc. at biological field.Therefore, Small-molecule probe being transformed and synthesized polymer is a kind of thinking that solves a lot of technical problems.
Summary of the invention
The technical problem to be solved in the present invention is, for above-mentioned defects such as in prior art, pH fluorescent probe solvent suitability are inadequate, freedom from jamming is poor, provides that a kind of solvent suitability is strong, freedom from jamming good and to H
+highstrung water-soluble polymer pH fluorescent probe PRAM, in addition, also provides the preparation method of this water-soluble polymer pH fluorescent probe PRAM.
The technical solution adopted for the present invention to solve the technical problems is as follows:
A kind of water-soluble polymer pH fluorescent probe PRAM, its structural formula is suc as formula shown in (1):
In formula (1), x and y are natural number, wherein, 1≤x≤10,200≤y≤700, and the span of the molecular weight of structure shown in formula (1) is (10000,50000).
In technical scheme of the present invention, the direct and electrophilic acryl of N atom on the water-soluble polymer pH fluorescent probe PRAM molecule volution of structure shown in above formula (1) is connected, and it is to H
+very responsive; When this water-soluble polymer pH fluorescent probe PRAM molecule runs into H
+time, its bottom lactam nucleus is opened, and this water-soluble polymer pH fluorescent probe PRAM molecule is by becoming hyperfluorescenceZeng Yongminggaoyingguang state without fluorescence state, and the required time of response is very short, in 10 seconds.
Again because the N atom on water-soluble polymer pH fluorescent probe PRAM molecule volution of the present invention is direct and electrophilic acryl is connected; introduced electron-withdrawing group; make the polarity of described water-soluble polymer pH fluorescent probe PRAM molecule become large, greatly promoted thus the solvent suitability of this water-soluble polymer pH fluorescent probe PRAM.
In metal ion, this water-soluble polymer pH fluorescent probe PRAM is only to Cu
2+more responsive, but it is to Cu
2+time of response longer, starting time approximately need be in about 4h, therefore Cu at short notice
2+can not this water-soluble polymer pH fluorescent probe PRAM be produced and be disturbed, therefore water-soluble polymer pH fluorescent probe PRAM of the present invention has good freedom from jamming.
The present invention also provides the preparation method of a kind of above-mentioned water-soluble polymer pH fluorescent probe PRAM, and the synthetic route of above-mentioned water-soluble polymer pH fluorescent probe PRAM is as follows:
One as the preparation method to above-mentioned water-soluble polymer pH fluorescent probe PRAM is improved, and comprises following processing step:
(1) Mrh preparation;
(2) reactants dissolved: the Mrh0.255～0.345g that adds acrylamide 2.55～3.45g, sodium bisulfite 0.04～0.06g and prepared by step (1) in 25.5～34.5ml water, stirs and makes above-mentioned reactants dissolved completely and obtain reactants dissolved liquid;
(3) building-up reactions: add 0.08～0.12g Sodium Persulfate to stir in the reactants dissolved liquid being prepared by step (2) and make it to dissolve completely and obtain reactant mixed solution, this reactant mixed solution reacts 3.5-6.5h at 38～42 DEG C in protection of inert gas;
(4) product precipitating: after building-up reactions in step (3) is complete, the aqueous solution after building-up reactions is slowly poured in 200～600ml acetone, be settled out incarnadine solid matter;
(5) product extracts: collect the incarnadine solid matter being settled out by step (4), after this incarnadine solid matter is extracted, is dried, obtain desired water-soluble polymer pH fluorescent probe PRAM.
In above-mentioned steps (2), first in 25.5～34.5ml water, add acrylamide 2.55～3.45g, sodium bisulfite 0.04～0.06g and Mrh0.255～0.345g, stirring makes above-mentioned reactants dissolved completely and obtains reactants dissolved liquid, in above-mentioned steps (3), in above-mentioned reactants dissolved liquid, add 0.08～0.12g Sodium Persulfate to stir and make it to dissolve completely and obtain reactant mixed solution, why sodium bisulfite and Sodium Persulfate are added respectively respectively in step (2) and (3), to generate the product that can serve as initiator because sodium bisulfite and Sodium Persulfate can react after mixing, to improve speed of reaction, also contribute to reduce energy consumption, if sodium bisulfite and Sodium Persulfate are joined in reactant simultaneously, can cause under the jejune condition of reaction conditions and react in advance, make reaction not exclusively, affect the raising of products collection efficiency.
In addition, in above-mentioned steps (3), the building-up reactions of reactant mixed solution under protection of inert gas is all higher to the requirement of temperature of reaction and time, if temperature is lower than 38 DEG C while reaction, the speed of this building-up reactions can be affected, and can cause reaction not exclusively; If when reaction in,, temperature exceeded 42 DEG C, can hinder carrying out smoothly of this building-up reactions, and may also can cause some other side reaction, affect the purity of product; If the reaction times is less than 3.5h, can cause reaction not thorough, if the reaction times exceedes 6.5h, not only can cause the unnecessary waste of production time, more seriously may cause the generation of side reaction.
In above-mentioned steps (4), the aqueous solution after building-up reactions is slowly poured in 200～600ml acetone, be settled out incarnadine solid matter, in this step, acetone is precipitating agent, for precipitating the macromolecule product of the aqueous solution after building-up reactions, i.e. water-soluble polymer pH fluorescent probe PRAM.
In above-mentioned steps (5), adopt acetone to carry out soxhlet extraction to incarnadine solid matter, and the time of soxhlet extraction is 11～13h, soxhlet extraction is good to the selectivity of water-soluble polymer pH fluorescent probe, be conducive to improve the extraction purity of water-soluble polymer pH fluorescent probe PRAM, and it is low to carry out soxhlet extraction power consumption, operating time is short, solvent load is few, and the equipment that carries out soxhlet extraction is simple, easy and simple to handle, facilitate water-soluble polymer pH fluorescent probe PRAM to extract carrying out smoothly of work.
One as the preparation method to above-mentioned water-soluble polymer pH fluorescent probe PRAM is improved, and the synthetic route of above-mentioned Mrh is as follows:
One as the preparation method to above-mentioned water-soluble polymer pH fluorescent probe PRAM is improved, and above-mentioned Mrh preparation comprises the steps:
Steps A: adopt rhodamine B to prepare rhodamine B hydrazides;
Step B: add rhodamine B hydrazides that 2.28g obtains by steps A and the tetrahydrofuran (THF) of 3.6-4.4ml in reaction vessels, grind and be cooled to 0 DEG C after 4-8min and obtain rhodamine B hydrazides suspension;
Step C: 0.45-0.55ml acrylate chloride is dissolved in and obtains acrylate chloride suspension in 1.8-2.2ml tetrahydrofuran (THF);
Step D: the acrylate chloride suspension being prepared by step C is joined in the rhodamine B hydrazides suspension being prepared by step B and react 5-8h generation precipitation, this precipitation is Mrh;
Step e: after Mrh generates, reaction suspension in filtration step D obtains Mrh, and this Mrh surface is cleaned to filtrate and become colorless, satisfactory Mrh obtained.
In the preparation process of above-mentioned Mrh, in step B and step C, all adopt tetrahydrofuran (THF) to prepare rhodamine B hydrazides suspension and acrylate chloride suspension as the dispersion agent of rhodamine B hydrazides and acrylate chloride respectively, reduce rhodamine B hydrazides and acrylate chloride and completed required time of dispersion process and energy, can prevent sedimentation and the cohesion of solids in suspension particle, contribute to rhodamine B hydrazides suspension and acrylate chloride suspension to carry out next step reaction.
In above-mentioned steps B, adopt tetrahydrofuran (THF) to disperse to form stable rhodamine B hydrazides suspension to 2.28g rhodamine B hydrazides, now the consumption of tetrahydrofuran (THF) is had to requirement, if the consumption of tetrahydrofuran (THF) is less than 3.6ml, can cause rhodamine B hydrazides to disperse not thorough, in the rhodamine B hydrazides suspension that may cause forming, there is the solid particulate not disperseing, be unfavorable for follow-up reaction; If the consumption of tetrahydrofuran (THF) is greater than 4.4ml, can cause material wastage, affect preparation cost.
In addition, in reaction vessels, add after rhodamine B hydrazides and tetrahydrofuran (THF), need to grind, to strengthen the dispersion effect of tetrahydrofuran (THF); In rhodamine B hydrazides suspension preparation process and process of lapping, can produce heat, for preventing that this heat from producing detrimentally affect to subsequent reactions, need after grinding 4-8min, mixed solution be cooled to 0 DEG C.
In step C, also adopt tetrahydrofuran (THF) to disperse 0.45-0.55ml acrylate chloride, same as above, now the consumption of tetrahydrofuran (THF) is had to requirement, if the consumption of tetrahydrofuran (THF) is less than 1.8ml, can cause acrylate chloride to disperse not thorough, in the acrylate chloride suspension that may cause forming, have the solid particulate not disperseing, be unfavorable for follow-up reaction; If the consumption of tetrahydrofuran (THF) is greater than 2.2ml, can cause material wastage, be unfavorable for minimizing of cost.
In step D, the acrylate chloride suspension preparing is joined in rhodamine B hydrazides suspension and reacted, now also there is to requirement in the reaction times, when the reaction times is during lower than 5h, can cause reaction not thorough, the one, affect the productive rate of Mrh, the 2nd, the rhodamine B hydrazides and the acrylate chloride that remain in reaction solution can exert an influence to the purity of Mrh, and then can have influence on solvent suitability and the interference free performance of Mrh.In the time that the reaction times exceedes 8h, can cause the meaningless prolongation in reaction times, be unfavorable for the saving in reaction times.
Improve as the one of the preparation method to above-mentioned water-soluble polymer pH fluorescent probe PRAM, in above-mentioned steps E, adopt tetrahydrofuran (THF) that Mrh surface is cleaned to filtrate and become colorless.The precipitation surface being generated by step e remains red liquid, in order to wash this red liquid, therefore adopt tetrahydrofuran (THF) that Mrh surface is cleaned to filtrate and become colorless.
In addition, in the technical scheme of the present invention, all being not specifically noted, all can be by adopting the conventional means in this area realize the technical program.
Therefore, the invention has the beneficial effects as follows a kind of water-soluble polymer pH fluorescent probe PRAM and preparation method thereof is provided, PRAM is strong to the suitability of solvent for this pH fluorescent probe, and freedom from jamming is good, and to H
+very responsive, the preparation method of this pH fluorescent probe PRAM is simple, and speed of reaction is fast, and energy consumption of reaction is little.
Brief description of the drawings
Below in conjunction with drawings and Examples, the invention will be further described, in accompanying drawing:
Fig. 1 is the nuclear magnetic resonance hydrogen spectruming determining result of water-soluble polymer pH fluorescent probe PRAM of the present invention;
Fig. 2 is the fluorescence curve of water-soluble polymer pH fluorescent probe PRAM solution of the present invention under condition of different pH, and wherein, in solution, the concentration of water-soluble polymer pH fluorescent probe PRAM is 0.2mg/ml.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with drawings and Examples, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Embodiment mono-:
A kind of water-soluble polymer pH fluorescent probe PRAM, its structural formula is as follows:
In said structure formula, x and y are natural number, wherein, 1≤x≤10,200≤y≤700, and the span of the molecular weight of structure shown in formula (1) is (10000,50000).
The present embodiment also provides the preparation method of above-mentioned water-soluble polymer pH fluorescent probe PRAM, and its synthetic route is as follows:
And the preparation method of above-mentioned water-soluble polymer pH fluorescent probe PRAM, comprises following processing step:
(1) Mrh preparation;
The synthetic route of this Mrh is as follows:
And this Mrh preparation comprises the steps:
Steps A: adopt rhodamine B to prepare rhodamine B hydrazides;
Step B: add rhodamine B hydrazides that 2.28g obtains by steps A and the tetrahydrofuran (THF) of 3.6ml in reaction vessels, grind and be cooled to 0 DEG C after 8min and obtain rhodamine B hydrazides suspension;
Step C: 0.45ml acrylate chloride is dissolved in and obtains acrylate chloride suspension in 1.8ml tetrahydrofuran (THF);
Step D: the acrylate chloride suspension being prepared by step C is joined in the rhodamine B hydrazides suspension being prepared by step B and react 5h generation precipitation, described precipitation is Mrh;
Step e: after Mrh generates, the reaction suspension in filtration step D obtains described Mrh, and adopt tetrahydrofuran (THF) that described Mrh surface is cleaned to filtrate and become colorless, obtain satisfactory Mrh;
(2) reactants dissolved: the Mrh0.255g that adds acrylamide 2.55g, sodium bisulfite 0.04g and prepared by step (1) in 25.5ml water, stirs and makes above-mentioned reactants dissolved completely and obtain reactants dissolved liquid;
(3) building-up reactions: add 0.08g Sodium Persulfate to stir in the reactants dissolved liquid being prepared by step (2) and make it to dissolve completely and obtain reactant mixed solution, described reactant mixed solution reacts 3.5h at 38 DEG C in protection of inert gas;
(4) product precipitating: after building-up reactions in step (3) is complete, the aqueous solution after building-up reactions is slowly poured in 200ml acetone, be settled out incarnadine solid matter;
(5) product extracts: collect the incarnadine solid matter being settled out by step (4), described incarnadine solid matter is extracted to 11h, after being dried, obtain described water-soluble polymer pH fluorescent probe PRAM.
Embodiment bis-:
The present embodiment and embodiment mono-are basic identical, and the two difference is:
The preparation method of above-mentioned water-soluble polymer pH fluorescent probe PRAM, comprises following processing step:
(1) Mrh preparation: this Mrh preparation comprises the steps:
Steps A: adopt rhodamine B to prepare rhodamine B hydrazides;
Step B: add rhodamine B hydrazides that 2.28g obtains by steps A and the tetrahydrofuran (THF) of 4ml in reaction vessels, grind and be cooled to 0 DEG C after 5min and obtain rhodamine B hydrazides suspension;
Step C: 0.5ml acrylate chloride is dissolved in and obtains acrylate chloride suspension in 2ml tetrahydrofuran (THF);
Step D: the acrylate chloride suspension being prepared by step C is joined in the rhodamine B hydrazides suspension being prepared by step B and react 6h generation precipitation, described precipitation is Mrh;
Step e: after Mrh generates, the reaction suspension in filtration step D obtains described Mrh, and adopt tetrahydrofuran (THF) that described Mrh surface is cleaned to filtrate and become colorless, obtain satisfactory Mrh;
(2) reactants dissolved: the Mrh0.3g that adds acrylamide 3g, sodium bisulfite 0.05g and prepared by step (1) in 30ml water, stirs and makes above-mentioned reactants dissolved completely and obtain reactants dissolved liquid;
(3) building-up reactions: add 0.1g Sodium Persulfate to stir in the reactants dissolved liquid being prepared by step (2) and make it to dissolve completely and obtain reactant mixed solution, described reactant mixed solution reacts 5h at 40 DEG C in protection of inert gas;
(4) product precipitating: after building-up reactions in step (3) is complete, the aqueous solution after building-up reactions is slowly poured in 400ml acetone, be settled out incarnadine solid matter;
(5) product extracts: collect the incarnadine solid matter being settled out by step (4), described incarnadine solid matter is extracted to 12h, after being dried, obtain described water-soluble polymer pH fluorescent probe PRAM.
Embodiment tri-:
The present embodiment and embodiment mono-are basic identical, and the two difference is:
The preparation method of above-mentioned water-soluble polymer pH fluorescent probe PRAM, comprises following processing step:
(1) Mrh preparation: this Mrh preparation comprises the steps:
Steps A: adopt rhodamine B to prepare rhodamine B hydrazides;
Step B: add rhodamine B hydrazides that 2.28g obtains by steps A and the tetrahydrofuran (THF) of 4.4ml in reaction vessels, grind and be cooled to 0 DEG C after 4min and obtain rhodamine B hydrazides suspension;
Step C: 0.55ml acrylate chloride is dissolved in and obtains acrylate chloride suspension in 2.2ml tetrahydrofuran (THF);
Step D: the acrylate chloride suspension being prepared by step C is joined in the rhodamine B hydrazides suspension being prepared by step B and react 8h generation precipitation, described precipitation is Mrh;
Step e: after Mrh generates, the reaction suspension in filtration step D obtains described Mrh, and adopt tetrahydrofuran (THF) that described Mrh surface is cleaned to filtrate and become colorless, obtain satisfactory Mrh;
(2) reactants dissolved: the Mrh0.345g that adds acrylamide 3.45g, sodium bisulfite 0.06g and prepared by step (1) in 34.5ml water, stirs and makes above-mentioned reactants dissolved completely and obtain reactants dissolved liquid;
(3) building-up reactions: add 0.12g Sodium Persulfate to stir in the reactants dissolved liquid being prepared by step (2) and make it to dissolve completely and obtain reactant mixed solution, described reactant mixed solution reacts 6.5h at 42 DEG C in protection of inert gas;
(4) product precipitating: after building-up reactions in step (3) is complete, the aqueous solution after building-up reactions is slowly poured in 600ml acetone, be settled out incarnadine solid matter;
(5) product extracts: collect the incarnadine solid matter being settled out by step (4), described incarnadine solid matter is extracted to 13h, after being dried, obtain described water-soluble polymer pH fluorescent probe PRAM.
Whether identical with above-mentioned formula (1) in order to verify the structure of the water-soluble polymer pH fluorescent probe PRAM that embodiment mono-to embodiment tri-obtains, the water-soluble polymer pH fluorescent probe PRAM that applicant obtains embodiment mono-to embodiment tri-has carried out nuclear magnetic resonance hydrogen spectruming determining, concrete outcome is shown in shown in accompanying drawing 1, and relevant 1H nuclear magnetic data is:
1h NMR (CDCl
3): δ=2.09 (1H), 1.52 (d, 2H).
With reference to the accompanying drawings 1 and relevant 1H nuclear magnetic data known, the structure embodying in the structure of the water-soluble polymer pH fluorescent probe PRAM that embodiment mono-to embodiment tri-obtains and above-mentioned formula (1) is identical.
In addition, in order to detect water-soluble polymer pH fluorescent probe PRAM that embodiment mono-to embodiment tri-obtains to H
+sensitivity and response interval, applicant is by the water-soluble polymer pH fluorescent probe PRAM aqueous solution of making soluble in water this water-soluble polymer pH fluorescent probe PRAM, wherein, the concentration of water-soluble polymer pH fluorescent probe PRAM is 0.2mg/ml, fluorescent emission wavelength is got 580nm, from accompanying drawing 2, this water-soluble polymer pH fluorescent probe PRAM is to H
+response sensitivity very high, and to H
+response interval be 6-8, be the meticulous pH fluorescent probe in 6-8 region therefore the water-soluble polymer pH fluorescent probe PRAM being obtained by embodiment mono-to embodiment tri-can be used as pH.
Should be understood that, for those of ordinary skills, can be improved according to the above description or convert, and all these improvement and conversion all should belong to the protection domain of claims of the present invention.
Claims (6)
1. a water-soluble polymer pH fluorescent probe PRAM, is characterized in that, its structural formula is suc as formula shown in (1):
In formula (1), x and y are natural number, wherein, 1≤x≤10,200≤y≤700, and the span of the molecular weight of structure shown in formula (1) is (10000,50000).
2. a preparation method of water-soluble polymer pH fluorescent probe PRAM claimed in claim 1, is characterized in that, the synthetic route of described water-soluble polymer pH fluorescent probe PRAM is as follows:
3. the preparation method of solubility pH fluorescent probe PRAM according to claim 2, is characterized in that, comprises following processing step:
(1) Mrh preparation;
(2) reactants dissolved: the Mrh0.255～0.345g that adds acrylamide 2.55～3.45g, sodium bisulfite 0.04～0.06g and prepared by step (1) in 25.5～34.5ml water, stirs and makes above-mentioned reactants dissolved completely and obtain reactants dissolved liquid;
(3) building-up reactions: add 0.08～0.12g Sodium Persulfate to stir in the reactants dissolved liquid being prepared by step (2) and make it to dissolve completely and obtain reactant mixed solution, described reactant mixed solution reacts 3.5-6.5h at 38～42 DEG C in protection of inert gas;
(4) product precipitating: after building-up reactions in step (3) is complete, the aqueous solution after building-up reactions is slowly poured in 200～600ml acetone, be settled out incarnadine solid matter;
(5) product extracts: collect the incarnadine solid matter being settled out by step (4), after described incarnadine solid matter is extracted, is dried, obtain described water-soluble polymer pH fluorescent probe PRAM.
4. the preparation method of solubility pH fluorescent probe PRAM according to claim 3, is characterized in that, the synthetic route of described Mrh is as follows:
5. according to the preparation method of the solubility pH fluorescent probe PRAM described in claim 3 or 4, it is characterized in that, described Mrh preparation comprises the steps:
Steps A: adopt rhodamine B to prepare rhodamine B hydrazides;
Step B: add rhodamine B hydrazides that 2.28g obtains by steps A and the tetrahydrofuran (THF) of 3.6-4.4ml in reaction vessels, grind and be cooled to 0 DEG C after 4-8min and obtain rhodamine B hydrazides suspension;
Step C: 0.45-0.55ml acrylate chloride is dissolved in and obtains acrylate chloride suspension in 1.8-2.2ml tetrahydrofuran (THF);
Step D: the acrylate chloride suspension being prepared by step C is joined in the rhodamine B hydrazides suspension being prepared by step B and react 5-8h generation precipitation, described precipitation is Mrh;
Step e: after Mrh generates, the reaction suspension in filtration step D obtains described Mrh, and described Mrh surface is cleaned to filtrate and become colorless, satisfactory Mrh obtained.
6. the preparation method of solubility pH fluorescent probe PRAM according to claim 5, is characterized in that, in described step e, adopts tetrahydrofuran (THF) that described Mrh surface is cleaned to filtrate and become colorless.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104910309A (en) * | 2015-06-15 | 2015-09-16 | 长春理工大学 | Water-soluble polymer Hg<2+> fluorescent probe and synthesis method thereof |
| CN104910316A (en) * | 2015-05-26 | 2015-09-16 | 郑州大学 | High-polymer colorimetric nano thin-film material and preparation method and application thereof in detecting Fe<3+> and pyrophosphate |
| CN109181681A (en) * | 2018-09-14 | 2019-01-11 | 济南大学 | It is a kind of to detect hypochlorous organosilicon macromolecule fluorescence probe and preparation method thereof |
| CN112358486A (en) * | 2021-01-07 | 2021-02-12 | 黄月金 | Detection of Pb in acidic aqueous solutions2+Fluorescent probe for ions and preparation method thereof |
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| CN101418215A (en) * | 2008-12-15 | 2009-04-29 | 苏州大学 | High molecule fluorescent detecting probe containing rhodamine gene and synthetic method |
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| CN101418215A (en) * | 2008-12-15 | 2009-04-29 | 苏州大学 | High molecule fluorescent detecting probe containing rhodamine gene and synthetic method |
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| CN104910316A (en) * | 2015-05-26 | 2015-09-16 | 郑州大学 | High-polymer colorimetric nano thin-film material and preparation method and application thereof in detecting Fe<3+> and pyrophosphate |
| CN104910309A (en) * | 2015-06-15 | 2015-09-16 | 长春理工大学 | Water-soluble polymer Hg<2+> fluorescent probe and synthesis method thereof |
| CN104910309B (en) * | 2015-06-15 | 2017-09-22 | 长春理工大学 | Water-soluble polymer Hg2+Fluorescence probe and its synthetic method |
| CN109181681A (en) * | 2018-09-14 | 2019-01-11 | 济南大学 | It is a kind of to detect hypochlorous organosilicon macromolecule fluorescence probe and preparation method thereof |
| CN109181681B (en) * | 2018-09-14 | 2020-03-13 | 济南大学 | Organic silicon polymer fluorescent probe for detecting hypochlorous acid and preparation method thereof |
| CN112358486A (en) * | 2021-01-07 | 2021-02-12 | 黄月金 | Detection of Pb in acidic aqueous solutions2+Fluorescent probe for ions and preparation method thereof |
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