CN104083549A - Application of rhizoma curculiginis extract in preparing anti-depression medicament - Google Patents
Application of rhizoma curculiginis extract in preparing anti-depression medicament Download PDFInfo
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- CN104083549A CN104083549A CN201410357087.8A CN201410357087A CN104083549A CN 104083549 A CN104083549 A CN 104083549A CN 201410357087 A CN201410357087 A CN 201410357087A CN 104083549 A CN104083549 A CN 104083549A
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- rhizoma curculiginis
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Abstract
The invention relates to application of a rhizoma curculiginis extract in preparing an anti-depression medicament, so as to effectively solve the problem of preparing anti-depression medicaments and realizing an application of a rhizoma curculiginis extract in preparing an anti-depression medicament. According to the application of the rhizoma curculiginis extract in preparing the anti-depression medicament, the rhizoma curculiginis extract is prepared by virtue of the following steps: crushing rhizoma curculiginis into coarse powder, soaking with ethanol, and performing reflux extraction; decompressing the filtrate to reclaim ethanol until the liquid does not have ethanol smell, concentrating, and dispersing the concentrate with distilled water to obtain a dispersant; loading the dispersant onto macroporous adsorption resin, eluting with distilled water, and removing the aqueous liquid; continuously eluting with ethanol, and removing the ethanol eluent; eluting with ethanol, collecting the ethanol eluent, reclaiming ethanol in reduced pressure, drying and crushing. The rhizoma curculiginis extract has an anti-depression effect and can be used for effectively preparing the anti-depression medicament, so that the application of the rhizoma curculiginis extract in preparing the anti-depression medicament can be realized.
Description
Technical field
The present invention relates to medicine, the particularly application of a kind of Rhizoma Curculiginis extract in preparing antidepressant drug.
Background technology
Rhizoma Curculiginis (formal name used at school: Curculigo orchioides Gaertn.), have another name called ground palm fibre (Sichuan, Guizhou), Rhizoma Curculiginis (Sichuan), mountain Radix Codonopsis (Fujian), Radix Scorzonerae Albicaulis (Yunnan), Radix Glehniae (Hainan), thatch claw, tragopogon pratensis l., belongs to Hypoxidaceae, florescence 4～JIUYUE.Medicinal plants, has the effect of reinforcing the kidney and supporting YANG, benefiting essence-blood, bone and muscle strengthening and promoting the circulation of blood detumescence.Along with improving constantly of Rhizoma Curculiginis drug value, the research of Rhizoma Curculiginis is valued by the people day by day, but so far there are no Rhizoma Curculiginis extract the open report of the application in preparing antidepressant drug, also have no the open report that Rhizoma Curculiginis is prepared Cure of depression medicine.
Summary of the invention
For above-mentioned situation, for overcoming the defect of prior art, the present invention's object is just to provide the application of a kind of Rhizoma Curculiginis extract in preparing antidepressant drug, can effectively solve the medicine of preparing depression, realizes the application of Rhizoma Curculiginis extract in preparing anti-depression drug.
The technical scheme that the present invention solves is, the application of Rhizoma Curculiginis extract in preparing antidepressant drug, and this Rhizoma Curculiginis extract is, Rhizoma Curculiginis is ground into coarse powder, by soak with ethanol, reflux, extract,, decompression filtrate recycling ethanol is extremely without alcohol taste, and be concentrated to the concentrated solution that 60 ℃ of relative densities are 1.20～1.30, concentrated solution adding distil water is dispersed into containing crude drug amount 0.5g/ml, obtains dispersion liquid, AB-8 type macroporous adsorbent resin on dispersion liquid, first use distilled water eluting, discard water liquid; The ethanol elution that is 10% by volumetric concentration again, discards volumetric concentration and is 10% ethanol elution; The ethanol elution that is finally 75% by volumetric concentration, the ethanol elution that collected volume concentration is 75%, decompression recycling ethanol, drying and crushing, obtains Rhizoma Curculiginis extract, and in Rhizoma Curculiginis extract, curculigo rhizome total glycoside mass content is more than 50%.
Preparation method of the present invention is simple, abundant raw materials, prepared Rhizoma Curculiginis extract has antidepressant effect, can be effective to prepare antidepressant drug, realize the application of Rhizoma Curculiginis extract in preparing antidepressant drug, opened up medical value and the new purposes of Rhizoma Curculiginis, and and then the new purposes of realization in preparing antidepressant drug.
The specific embodiment
Below in conjunction with concrete condition, the specific embodiment of the present invention is elaborated.
The present invention is in concrete enforcement, the application of Rhizoma Curculiginis extract in preparing antidepressant drug, this Rhizoma Curculiginis extract is, Rhizoma Curculiginis was ground into the coarse powder of 20-30 mesh sieve, by 10 times of Rhizoma Curculiginis weight, volumetric concentration is 80% soak with ethanol 0.5h, reflux, extract, 2 times, each 1.5h, filter, merging filtrate, decompression recycling ethanol is extremely without alcohol taste, and be concentrated to the concentrated solution that 60 ℃ of relative densities are 1.20～1.30, concentrated solution adding distil water is dispersed into containing crude drug amount 0.5g/ml, obtain dispersion liquid, AB-8 type macroporous adsorbent resin on dispersion liquid, the ratio of upper column quantity and resin volume is 1 ︰ 12, resin column blade diameter length ratio is 1 ︰ 12, first use 4 times of column volume distilled water eluting, discard water liquid, the ethanol elution that is 10% by the volumetric concentration of 4 times of column volumes again, discards volumetric concentration and is 10% ethanol elution, the ethanol elution that is finally 75% by the volumetric concentration of 10 times of column volumes, the ethanol elution that collected volume concentration is 75%, decompression recycling ethanol, 60 ℃ of drying and crushing, obtain Rhizoma Curculiginis extract, and in Rhizoma Curculiginis extract, curculigo rhizome total glycoside mass content is more than 50%.
Rhizoma Curculiginis extract of the present invention has antidepressant effect, can be effective to prepare antidepressant drug, realize the application of Rhizoma Curculiginis extract in preparing antidepressant drug, and then realize the application of Rhizoma Curculiginis extract in preparing Cure of depression medicine, and having obtained valid certificates through test, related tests data is as follows.
One, the impact of Rhizoma Curculiginis extract on mice behavioral despair depression model
1 experiment material
1.1 laboratory animal
Clean level mice, Kunming kind, male, 18-22g, SPF level, is provided by Lukang Medical Co., Ltd., Shandong.Quality certification numbering 0017173; Laboratory quality certification SYXK (Henan) 2010-001.
1.2 medicine and reagent
Rhizoma Curculiginis extract of the present invention; Fluoxetine Hydrochloride, commercially available; Sodium carboxymethyl cellulose, Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd.
1.3 experimental apparatus
Electronic balance, Shanghai Precision Scientific Apparatus Co., Ltd produces, model YP420N; Mice autonomic activities tester, Chengdu TME Technology Co., Ltd., model ZZ-6; Mouse tail suspension instrument, mouse tail suspension tester, Chengdu TME Technology Co., Ltd., model T-200.
2 experimental techniques
2.1 grouping and administrations
Group technology: get 60 of body weight 18-22g male mouse of kunming, be divided into 5 groups with being about to mice: blank group, large, medium and small dosage Rhizoma Curculiginis extract group, fluoxetine Hydrochloride group.
Dosage: 0.6%CMC preparation: take CMC0.6g, be made into 100ml with distilled water.Large, medium and small dosage Rhizoma Curculiginis extract group dosage is respectively 300mgkg
-1, 150mgkg
-1, 75mgkg
-1(dosage is determined in conjunction with the content of total glycosides in clinical Rhizoma Curculiginis consumption and Rhizoma Curculiginis), collocation method: take respectively Rhizoma Curculiginis extract 756mg, 378mg, 189mg, dissolve with the 0.6%CMC of 50mL, concentration is 15mgmL
-1, 7.5mgmL
-1, 3.75mgmL
-1.Fluoxetine Hydrochloride (10mgkg
-1, concentration 0.5mgmL
-1, be equivalent to 15 times of quantity), collocation method: get 2.5 of fluoxetine tablets, dissolve with the 0.6%CMC of 50mL.Administration volume is 0.2mL/10g.
Medication: each treated animal gives relative medicine on the 1st day in grouping.Fluoxetine Hydrochloride group gavage fluoxetine Hydrochloride suspension 10mgkg
-1, large, medium and small dosage Rhizoma Curculiginis extract group is respectively by the large, medium and small dosage Rhizoma Curculiginis extract of group gavage 300mgkg
-1, 150mgkg
-1, 75mgkg
-1.Blank group and model group be gavage same volume distilled water respectively, and every day, gastric infusion was 1 time, successive administration 7d.
2.2 observation project and detection methods
2.2.1 autonomic activities number of times test
1h after last administration, puts into autonomic activities instrument by each group mice, and the 2min that first conforms, then measures mice autonomic activities number of times in 5min.
2.2.2 Tail suspension test test
1h after last administration, with medical adhesive tape, mice tail end 2cm place is fixed on metal bar, make mice be reversal of the natural order of things state, metal bar is sticked on outstanding tail instrument casing, from bottom 5cm, adapt to after 2min, record motionless (mice stops struggling, or only the has tiny limb motion) time of mice in 4min aloft.
2.2.3 mouse swimming test test
1h after mice last gastric infusion, mice is put down gently in 3000ml beaker, add 2000ml water, water temperature (25 ± 1) ℃, adapt to after 2min, survey the time of (be that mice stops struggling in water, or mice is floating state, only has tiny limb motion to keep head to keep afloat) of motionless state in mice 4min.
3 experimental results
The impact of table 1 Rhizoma Curculiginis extract on mice behavioral despair depression model autonomic activities, outstanding tail dead time and non-swimming time
Note: with the comparison of blank group, * P<0.05, * * P<0.01
With blank group ratio, large, medium and small dosage Rhizoma Curculiginis extract group and fluoxetine Hydrochloride group have no significant effect (P>0.05) to mice autonomic activities, can tentatively get rid of the excitation of medicine centering pivot nervous system and cause the possibility of false positive results.With blank group ratio, heavy dose of Rhizoma Curculiginis extract group and fluoxetine Hydrochloride group all can significantly shorten the mouse tail suspension dead time (P<0.01), and middle dosage Rhizoma Curculiginis extract group can obviously shorten the mouse tail suspension dead time (P<0.05); Heavy dose of Rhizoma Curculiginis extract group can obviously shorten mice non-swimming time (P<0.05), and fluoxetine Hydrochloride group can significantly shorten mice non-swimming time (P<0.01).
Two, the impact of Rhizoma Curculiginis extract on reserpine induction depression model mice
1 experiment material
1.1 laboratory animal
Clean level mice, Kunming kind, male, 18-22g, SPF level, is provided by Lukang Medical Co., Ltd., Shandong.Quality certification numbering 0017173; Laboratory quality certification SYXK (Henan) 2010-001.
1.2 medicine and reagent
Rhizoma Curculiginis extract of the present invention; Fluoxetine Hydrochloride, commercially available; Sodium carboxymethyl cellulose, Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd; Reserpine injection, Tianjin Jin Yao aminoacid company limited.
1.3 experimental apparatus
Electronic balance, Shanghai Precision Scientific Apparatus Co., Ltd produces, model YP420N; Contactless electronic clinical thermometer, Guangzhou kind Yu Jin Xin Bao Electronics Co., Ltd., model JXB-183; Skilful honey that depilatory cream, Guangzhou Yi Baiya cosmetics company limited, article No. T19.
2 experimental techniques
2.1 grouping and administrations
Group technology: get 72 of body weight 18-22g male mouse of kunming, be divided into 6 groups with being about to mice: blank group, large, medium and small dosage Rhizoma Curculiginis extract group, fluoxetine Hydrochloride group.
Dosage: 0.6%CMC preparation: take CMC0.6g, be made into 100ml with distilled water.Large, medium and small dosage Rhizoma Curculiginis extract group dosage is respectively 300mgkg
-1, 150mgkg
-1, 75mgkg
-1(dosage is determined in conjunction with the content of total glycosides in clinical Rhizoma Curculiginis consumption and Rhizoma Curculiginis), collocation method: take respectively Rhizoma Curculiginis extract 756mg, 378mg, 189mg, dissolve with the 0.6%CMC of 50mL, concentration is 15mgmL
-1, 7.5mgmL
-1, 3.75mgmL
-1.Fluoxetine Hydrochloride (10mgkg
-1, concentration 0.5mgmL
-1, be equivalent to 15 times of quantity), collocation method: get 2.5 of fluoxetine tablets, dissolve with the 0.6%CMC of 50mL.Administration volume is 0.2mL/10g.
Medication: each treated animal gives relative medicine on the 1st day in grouping.Fluoxetine Hydrochloride group gavage fluoxetine Hydrochloride suspension 10mgkg
-1, large, medium and small dosage Rhizoma Curculiginis extract group is respectively by the large, medium and small dosage Rhizoma Curculiginis extract of group gavage 300mgkg
-1, 150mgkg
-1, 75mgkg
-1.Blank group and model group be gavage same volume distilled water respectively, and every day, gastric infusion was 1 time, successive administration 7d.
2.2 observation items and detection method
2.2.1 blepharoptosis degree is observed
1h after last administration, lumbar injection reserpine 4mg/kg, observes after 1h: (1) ptosis: mice is put and observes on the top of the shelf 15s, observe animal and whether occur blepharoptosis and blepharoptosis degree and mark.0 minute, eyelid was not closed; Within 1 minute, eyelid closes 1/4; 2 minutes, eyelid closed 1/2; 3 minutes, eyelid closed 3/4; 4 minutes, eyelid Close All.
2.2.2 motion can not be observed
The circular blank sheet of paper that animal is placed on to diameter 7.5cm is central, observes 15s, respectively organizes mice and still stays in the number in circle.
2.2.3 body temperature is observed
After 4h, with clinical thermometer, aim at depilation place of mice forehead and measure mouse temperature, respectively organize body temperature value.
3 experimental techniques
The impact of table 2 Rhizoma Curculiginis extract on reserpine induction depression model mice
Note: with the comparison of blank group, * P<0.05, * * P<0.01
With blank group ratio, model group mice blepharoptosis degree significantly increases (P<0.01), the rate that goes too far significantly reduces (P<0.01), body temperature value significantly reduces (P<0.01), illustrates and makes the success of reserpine depression model mice.With model group ratio, heavy dose of Rhizoma Curculiginis extract group can obviously alleviate model mice blepharoptosis degree (P<0.05), and fluoxetine Hydrochloride group can significantly alleviate model mice blepharoptosis degree (P<0.01); Heavy dose of Rhizoma Curculiginis extract group can obviously increase the model mice rate (P<0.05) that goes too far, and fluoxetine Hydrochloride group can significantly increase the model mice rate (P<0.01) that goes too far; Fluoxetine Hydrochloride group can significantly increase model mice body temperature (P<0.01).
Above-mentioned testing data fully shows that in Rhizoma Curculiginis extract of the present invention, general flavone content reaches more than 50%, there is good antidepressant effect, be effective to prepare antidepressant drug, realize the application of Rhizoma Curculiginis extract in preparing antidepressant drug, and opened up the new way for the treatment of depression medication, and realize the application of Rhizoma Curculiginis extract in preparing Cure of depression medicine, and through clinical, the 55 routine patients with depression way of taking medicine is routinely taken, every day 3 times, each 1.5-2.0g, serve on 20-30 days, measure curative effect, depression all has improvement in various degree, in tested case, effective percentage is almost 100%, show no matter be through animal experiment, or through clinical verification, all prove that Rhizoma Curculiginis extract has the effect of antidepressant, new purposes and the medical value of Rhizoma Curculiginis have been opened up, there is great practical value, that one on Chinese medicine is innovated greatly.
Claims (1)
1. the Rhizoma Curculiginis extract application in preparing antidepressant drug, this Rhizoma Curculiginis extract is, Rhizoma Curculiginis was ground into the coarse powder of 20-30 mesh sieve, by 10 times of Rhizoma Curculiginis weight, volumetric concentration is 80% soak with ethanol 0.5h, reflux, extract, 2 times, each 1.5h, filter, merging filtrate, decompression recycling ethanol is extremely without alcohol taste, and be concentrated to the concentrated solution that 60 ℃ of relative densities are 1.20～1.30, concentrated solution adding distil water is dispersed into containing crude drug amount 0.5g/ml, obtain dispersion liquid, AB-8 type macroporous adsorbent resin on dispersion liquid, the ratio of upper column quantity and resin volume is 1:12, resin column blade diameter length ratio is 1:12, first use 4 times of column volume distilled water eluting, discard water liquid, the ethanol elution that is 10% by the volumetric concentration of 4 times of column volumes again, discards volumetric concentration and is 10% ethanol elution, the ethanol elution that is finally 75% by the volumetric concentration of 10 times of column volumes, the ethanol elution that collected volume concentration is 75%, decompression recycling ethanol, 60 ℃ of drying and crushing, obtain Rhizoma Curculiginis extract, and in Rhizoma Curculiginis extract, curculigo rhizome total glycoside mass content is more than 50%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547326A (en) * | 2015-01-30 | 2015-04-29 | 浙江省中药研究所有限公司 | Antidepressant traditional Chinese medicine composition for warmly invigorating kidney yang as well as preparation method and application thereof |
| CN104983985A (en) * | 2015-08-04 | 2015-10-21 | 山东中医药大学附属医院 | Chinese herba preparation for treating depression |
| CN105663506A (en) * | 2016-03-01 | 2016-06-15 | 南京中医药大学 | Traditional Chinese medicine extract with anti-depression effect and application thereof |
| CN106176899A (en) * | 2016-08-24 | 2016-12-07 | 天津中新药业研究中心 | A kind of preparation method and applications of bastard tamarind legume extract |
| CN107441166A (en) * | 2017-08-12 | 2017-12-08 | 河南中医药大学 | Application of the cinnamon oil in treatment behavior despair antidepressant agents are prepared |
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| CN101134041A (en) * | 2007-08-03 | 2008-03-05 | 中国科学院昆明植物研究所 | Antidepressant and uses thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101134041A (en) * | 2007-08-03 | 2008-03-05 | 中国科学院昆明植物研究所 | Antidepressant and uses thereof |
Non-Patent Citations (1)
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| 汪天明: "中华仙茅的化学成分研究", 《中国优秀硕士论文全文数据库 医药卫生科技辑》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104547326A (en) * | 2015-01-30 | 2015-04-29 | 浙江省中药研究所有限公司 | Antidepressant traditional Chinese medicine composition for warmly invigorating kidney yang as well as preparation method and application thereof |
| CN104547326B (en) * | 2015-01-30 | 2018-04-10 | 浙江省中药研究所有限公司 | Antidepressant Chinese medicine composition of warming and invigorating kidney Yang and preparation method thereof and purposes |
| CN104983985A (en) * | 2015-08-04 | 2015-10-21 | 山东中医药大学附属医院 | Chinese herba preparation for treating depression |
| CN104983985B (en) * | 2015-08-04 | 2016-03-16 | 山东中医药大学附属医院 | A kind of Chinese medicine preparation of Cure of depression |
| CN105663506A (en) * | 2016-03-01 | 2016-06-15 | 南京中医药大学 | Traditional Chinese medicine extract with anti-depression effect and application thereof |
| CN106176899A (en) * | 2016-08-24 | 2016-12-07 | 天津中新药业研究中心 | A kind of preparation method and applications of bastard tamarind legume extract |
| CN107441166A (en) * | 2017-08-12 | 2017-12-08 | 河南中医药大学 | Application of the cinnamon oil in treatment behavior despair antidepressant agents are prepared |
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Application publication date: 20141008 |