CN104076384A - Detection method of 99mTc-MDP brine phase - Google Patents
Detection method of 99mTc-MDP brine phase Download PDFInfo
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- CN104076384A CN104076384A CN201310106119.2A CN201310106119A CN104076384A CN 104076384 A CN104076384 A CN 104076384A CN 201310106119 A CN201310106119 A CN 201310106119A CN 104076384 A CN104076384 A CN 104076384A
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- mdp
- chromatographic paper
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- developping agent
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Abstract
The invention relates to a detection method of a 99mTc-MDP brine phase. According to the method, an existing Na I single-channel spectrometer counter is adopted for measurement and counting for detection, and the brine phase is detected through polyamide thin layer chromatography paper instead of Xinhua-1 chromatography paper. Compared with the prior art, the polyamide thin layer chromatography paper has the advantages of being high in sensitivity, high in resolving power, fast and convenient to operate, and the like. The detection result is close to the true value, and great advantages are brought to result judgment.
Description
Technical field
The present invention relates to a kind of radioisotopic detection method, especially relate to a kind of
99mthe detection method of Tc-MDP salt water.
Background technology
99mthe full name of Tc-MDP be technetium [
99mtc] MDP parenteral solution, nucleic: technetium [
99mtc]; Label: injection methylenediphsphonate and stannous chloride.
Be mainly used in whole body or local bone video picture, diagnosis bone and joint diseases, former or metastatic tumor of bone disease etc.
99mtc-MDP is the comparatively ideal skeletal imaging agent of generally acknowledging at present.By chemisorption, be incorporated into hydroxylapatite crystal (hydroxypatitecrystal) surface in the inorganic constituents of bone, immature collagen in bone ash, also right in addition
99mtc-MDP has higher affinity.Affect bone dense poly-
99mtc-MDP principal element is the blood confession of bone and the formation speed of new bone.In addition, this product can also be positioned the cardiac muscle cell of infraction or the soft tissue of calcification, enters muscle, cartilage, blood vessel and internal organs.
99mthe quality internal enterprise standards middle term of Tc-MDP is a lot, but what the most directly can reflect its quality is radio chemistry purity.Therefore, radio chemistry purity is accurate, accurately just rather important.Therefore detection method just must accomplish to approach as far as possible true value, convenient judgement.
99mthe detection method of Tc-MDP radio chemistry purity is dual system method in pharmacopeia, i.e. 85% methyl alcohol phase and sodium chloride salt water.This two-phase requires No. 1 chromatographic paper of Dou Shi Xinhua using.Then use NaI single-channel spectrometer counter, read counting, then calculate its Rf value and radio chemistry purity.
Use the shortcoming of No. 1 test paper detecting method of Xinhua:
1. No. 1 test paper of Xinhua easily collapses in the process of launching, and causes data corruption, inhomogeneous, inaccurate, is difficult to tell free technetium present position;
2. No. 1 test paper of Xinhua dries needs the longer time.The time that radiomaterial leaks cruelly in environment is like this just partially long, and it is just bigger than normal that operating personnel accept radiological dose.
Summary of the invention
Object of the present invention is exactly to provide a kind of highly sensitive in order to overcome the defect of above-mentioned prior art existence, and resolving power is strong, fast, easy to operate
99mthe detection method of Tc-MDP salt water.
Object of the present invention can be achieved through the following technical solutions: a kind of
99mthe detection method of Tc-MDP salt water, is characterized in that, the method comprises the following steps:
(1) prepare chromatographic paper
Adopt polyam ide TLC chromatography as chromatographic paper, polyam ide TLC chromatographic paper is cut into wide 0.5cm, the paper slip of long 10cm, apart from 1.0cm place, bottom picture straight line as baseline, as point of sample;
(2) point sample
Developping agent is placed in to chromatography cylinder, and cover lid, places, and makes developping agent volatilization make to be full of in chromatography cylinder saturated vapour, with kapillary draw technetium [
99mtc] MDP parenteral solution, drip on polyam ide TLC chromatographic paper baseline intermediate point, under infrared lamp, dry, the chromatographic paper that point sample is good is put into developping agent, lean in chromatography casing wall, wait and be expanded to the place's taking-up of fast forward position, record position, forward position, dry;
(3) measuring and calculating
From one end 0.5cm of chromatographic paper draw base line, cut, remainder is evenly divided into 10 equal portions, detects and record data result of calculation.
Described developping agent is sodium chloride solution.
Compared with prior art, the present invention still adopts NaI single-channel spectrometer counter measures counting, but salt water is not being used No. 1 chromatographic paper of Xinhua, but uses polyam ide TLC chromatographic paper.Using polyam ide TLC chromatographic paper method of the present invention to substitute No. 1 chromatographic paper method of used Xinhua detects
99mthe advantages such as Tc-MDP salt water radio chemistry purity has following benefit: use polyam ide TLC chromatographic paper to have highly sensitive, resolving power is strong, quick, easy to operate.Make the result detecting approach true value, to result, judgement brings very big benefit.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of
99mthe detection method of Tc-MDP salt water, the method adopts existing NaI single-channel spectrometer counter measures counting to detect, but salt water is not being used No. 1 chromatographic paper of Xinhua, but use polyam ide TLC chromatographic paper.Specifically comprise the following steps:
(1) prepare chromatographic paper
Adopt polyam ide TLC chromatography as chromatographic paper, polyam ide TLC chromatographic paper is cut into wide 0.5cm, the paper slip of long 10cm, apart from 1.0cm place, bottom picture straight line as baseline, as point of sample;
(2) point sample
Developping agent sodium chloride solution is placed in to chromatography cylinder, and cover lid, places, and makes developping agent volatilization make to be full of in chromatography cylinder saturated vapour, with kapillary draw technetium [
99mtc] MDP parenteral solution, drip on polyam ide TLC chromatographic paper baseline intermediate point, under infrared lamp, dry, the chromatographic paper that point sample is good is put into developping agent, lean in chromatography casing wall, wait and be expanded to the place's taking-up of fast forward position, record position, forward position, dry;
(3) measuring and calculating
From one end 0.5cm of chromatographic paper draw base line, cut, remainder is evenly divided into 10 equal portions, detects and record data result of calculation.
Claims (2)
1. one kind
99mthe detection method of Tc-MDP salt water, is characterized in that, the method comprises the following steps:
(1) prepare chromatographic paper
Adopt polyam ide TLC chromatography as chromatographic paper, polyam ide TLC chromatographic paper is cut into wide 0.5cm, the paper slip of long 10cm, apart from 1.0cm place, bottom picture straight line as baseline, as point of sample;
(2) point sample
Developping agent is placed in to chromatography cylinder, and cover lid, places, and makes developping agent volatilization make to be full of in chromatography cylinder saturated vapour, with kapillary draw technetium [
99mtc] MDP parenteral solution, drip on polyam ide TLC chromatographic paper baseline intermediate point, under infrared lamp, dry, the chromatographic paper that point sample is good is put into developping agent, lean in chromatography casing wall, wait and be expanded to the place's taking-up of fast forward position, record position, forward position, dry;
(3) measuring and calculating
From one end 0.5cm of chromatographic paper draw base line, cut, remainder is evenly divided into 10 equal portions, detects and record data result of calculation.
2. according to claim 1 a kind of
99mthe detection method of Tc-MDP salt water, is characterized in that, described developping agent is sodium chloride solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310106119.2A CN104076384A (en) | 2013-03-28 | 2013-03-28 | Detection method of 99mTc-MDP brine phase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201310106119.2A CN104076384A (en) | 2013-03-28 | 2013-03-28 | Detection method of 99mTc-MDP brine phase |
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| CN104076384A true CN104076384A (en) | 2014-10-01 |
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| CN201310106119.2A Pending CN104076384A (en) | 2013-03-28 | 2013-03-28 | Detection method of 99mTc-MDP brine phase |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4058593A (en) * | 1976-01-28 | 1977-11-15 | Minnesota Mining & Mfg | Technetium-labeled bone scanning agents |
| JPS5395975A (en) * | 1977-01-28 | 1978-08-22 | Daiichi Radioisotope Lab | Novel technetium labeled compound |
| CN1280013A (en) * | 1999-07-07 | 2001-01-17 | 北京师范大学应用化学研究所 | Brain perfusion developer 99m TCN (CHDT)2 and its preparing method |
| CN1331081A (en) * | 2000-06-28 | 2002-01-16 | 北京师范大学 | Tetraphosphine quatorphosphonium salt, its preparing process, and two-step tetraphosphine medicine kit and its application |
| CN1418884A (en) * | 2002-12-19 | 2003-05-21 | 北京师范大学 | 99TcMN dimercaptosuccinate compound, its preparation process and application in nucleic medicine science |
| US20040044065A1 (en) * | 2000-04-20 | 2004-03-04 | Dolphin David H. | Radiometal complexes of 2-pyrrolylthiones and their use as radiopharmaceuticals for imaging and therapy |
| CN102253174A (en) * | 2011-04-27 | 2011-11-23 | 江苏省原子医学研究所 | Method of chemical kinetics research for receptor developing agent labels |
-
2013
- 2013-03-28 CN CN201310106119.2A patent/CN104076384A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4058593A (en) * | 1976-01-28 | 1977-11-15 | Minnesota Mining & Mfg | Technetium-labeled bone scanning agents |
| JPS5395975A (en) * | 1977-01-28 | 1978-08-22 | Daiichi Radioisotope Lab | Novel technetium labeled compound |
| CN1280013A (en) * | 1999-07-07 | 2001-01-17 | 北京师范大学应用化学研究所 | Brain perfusion developer 99m TCN (CHDT)2 and its preparing method |
| US20040044065A1 (en) * | 2000-04-20 | 2004-03-04 | Dolphin David H. | Radiometal complexes of 2-pyrrolylthiones and their use as radiopharmaceuticals for imaging and therapy |
| CN1331081A (en) * | 2000-06-28 | 2002-01-16 | 北京师范大学 | Tetraphosphine quatorphosphonium salt, its preparing process, and two-step tetraphosphine medicine kit and its application |
| CN1418884A (en) * | 2002-12-19 | 2003-05-21 | 北京师范大学 | 99TcMN dimercaptosuccinate compound, its preparation process and application in nucleic medicine science |
| CN102253174A (en) * | 2011-04-27 | 2011-11-23 | 江苏省原子医学研究所 | Method of chemical kinetics research for receptor developing agent labels |
Non-Patent Citations (3)
| Title |
|---|
| CAROLINE M. CUNNANE ET AL.: "Determination of the radiochemical purity of 99mTc medronate injection by thin layer chromatography on iTLC-SG: effect of medronate concentration on the value measured", 《JOURNAL OF LABELLED COMPOUNDS AND RADIOPHARMACEUTICALS》 * |
| 郭勇: "《现代生化技术》", 28 February 2005, 科学出版社 * |
| 黄天森 等: "99mTc－MDP放化纯测定法的改进", 《核技术》 * |
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Application publication date: 20141001 |