CN104073350A - Method for extracting volatile oil from small schizonepeta - Google Patents
Method for extracting volatile oil from small schizonepeta Download PDFInfo
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- CN104073350A CN104073350A CN201410266373.3A CN201410266373A CN104073350A CN 104073350 A CN104073350 A CN 104073350A CN 201410266373 A CN201410266373 A CN 201410266373A CN 104073350 A CN104073350 A CN 104073350A
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Abstract
The invention discloses a method for extracting volatile oil from small schizonepeta, relating to an extraction process of volatile oil. The method disclosed by the invention comprises the following steps: performing raw material treatment, soaking, enzymolysis, distillation, extraction and drying to obtain the volatile oil of small schizonepeta. The process is simple and reasonable, the operation time is short, the extraction rate of the volatile oil is high, the application range is wide, and the method has a great practical value.
Description
Technical field
The present invention relates to a kind of extraction process of volatile oil, a kind of method that particularly biological enzymolysis technology combines with underpressure distillation is extracted volatile oil from little schizonepeta.
Background technology
Little schizonepeta is the dry aerial parts of labiate schizonepeta, and containing volatile oil, in oil, main component is D-menthone, racemization piperitone.Can be used for that treatment is caught cold, the measles initial stage thoroughly send out smooth, frying charcoal can hemostasis, uterine bleeding, have blood in stool.The general lossless mustard fringe of using, has sweat with schizonepeta, enters blood system Herba Schizonepetae charcoal.
Volatile oil claims again essential oil, and English is essential oils, be present in a class in plant have aromatic odour, can with wet distillation out and with the general name of the not miscible volatility oily composition of water.Volatile oil is a mixture, and its component is comparatively complicated.Mainly by steam distillation and milling process, produce essential oil.Common with ter penoids in volatile oil component, in addition, still contain small molecules fatty compounds and small molecules aromatics.
Through many, studies have shown that, volatile oil has very strong pharmacological action.In document < < aromatics traditional medicine volatile oil anti-microbial effect progress > >, say: traditional medicine volatile oil has anti-inflammatory, antianaphylaxis, antimicrobial, anti-mutation and anticancer, anthelmintic action, enzyme inhibition, to the effect of central nervous system, to effect of respiratory system etc.
At present, in China, about extract the method for volatile oil from little schizonepeta, seldom seeing, is mainly steam distillation, solvent-extraction process, microwave extraction method etc., and these procedures are single, extraction efficiency is low.The method that the present invention adopts biological enzymolysis technology to combine with underpressure distillation is extracted little Herba Schizonepetae volatile oil, and the extraction yield of the volatile oil that is is greatly improved.
Summary of the invention
The object of this invention is to provide a kind of method of extracting volatile oil from little schizonepeta, the method is simple to operate, the used time is shorter, and key is that the method that the extraction yield of volatile oil extracts volatile oil compared with other wants high, and cost-saving, is applicable to suitability for industrialized production.
To achieve these goals, the present invention is by the following technical solutions:
(1) fresh little schizonepeta is cleaned up, remove root, be cut into 2-3mm segment;
(2) get broken section of little schizonepeta, add in extractor, with distilled water immersion, and stirred solution;
(3) then add the biological enzyme reagent of broken section of weight 0.5%-2% of little schizonepeta to carry out enzymolysis processing;
(4) after enzymolysis completes, adopt the technique of underpressure distillation, collect distillate, add distilled water and repeat to distill 1-2 time;
(5) merge distillate, use distillate volume 2-5 extracted with diethyl ether doubly, and add the desiccant dehydration of broken section of weight 1/5-1/4 of little schizonepeta;
(6) heating in water bath that adopts temperature to be controlled at 37-41 ℃ reclaims ether, obtains little Herba Schizonepetae volatile oil.
The amount that adds distilled water described in step (2) is 5-10 times of broken section of weight of little schizonepeta, and soak time is 20-40min.
Biological enzyme described in step (3) is a kind of in cellulase, amylase, hemicellulase and polygalacturonase, and Temperature Setting during enzymolysis is at 30-45 ℃, and enzymolysis time is 1-3 hour.
Underpressure distillation described in step (4), temperature is controlled at 50-60 ℃.
Siccative described in step (5) is a kind of in anhydrous sodium sulphate, anhydrous magnesium sulfate.
Adopt this law to extract the volatile oil in little schizonepeta, employing be the method that biological enzymolysis technology combines with underpressure distillation, its technological process is simple, reasonable, volatile oil extraction yield is high, the operating time is short, applied range, have very high practical value.
Below in conjunction with embodiment, further illustrate the present invention, but the scope of protection of present invention is not limited to following embodiment.
Embodiment
Embodiment 1:
First the little schizonepeta cleaning up is removed to root, is cut into the segment that length is 2mm, get broken section of the little schizonepeta of 100g, be placed in extractor, add the distilled water immersion 30 minutes of 500 milliliters, during every 10 minutes, use glass stick stirred solution.Soak after 30 minutes, in extractor, add 2g cellulase, under the condition of 35 ℃, enzymolysis is 1 hour.After enzymolysis completes, underpressure distillation, during distillation, temperature is controlled at 55 ℃, collects distillate, add distilled water, repeat distillation procedure 1 time, merge twice distillate, add the extracted with diethyl ether of 3 times of distillate volumes, and add 20g anhydrous sodium sulfate dehydration, ether is reclaimed in 37 ℃ of water-baths, obtains little Herba Schizonepetae volatile oil, and extraction yield is 52.6%.
Embodiment 2:
First the little schizonepeta cleaning up is removed to root, is cut into the segment that length is 2mm, get broken section of the little schizonepeta of 150g, be placed in extractor, add the distilled water immersion 40 minutes of 750 milliliters, during every 10 minutes, use glass stick stirred solution.Soak after 40 minutes, in extractor, add 3g cellulase, under the condition of 35 ℃, enzymolysis is 1 hour.After enzymolysis completes, underpressure distillation, during distillation, temperature is controlled at 55 ℃, collects distillate, add distilled water, repeat distillation procedure 1 time, merge twice distillate, add the extracted with diethyl ether of 3 times of distillate volumes, and add 30g anhydrous sodium sulfate dehydration, ether is reclaimed in 37 ℃ of water-baths, obtains little Herba Schizonepetae volatile oil, and extraction yield is 51.56%.
Claims (7)
1. from little schizonepeta, extract a method for volatile oil, it is characterized in that, it is mainly to adopt following steps:
(1) fresh little schizonepeta is cleaned up, remove root, be cut into 2-3mm segment;
(2) get broken section of little schizonepeta, add in extractor, with distilled water immersion, and stirred solution;
(3) then add the biological enzyme reagent of broken section of weight 0.5%-2% of little schizonepeta to carry out enzymolysis processing;
(4) after enzymolysis completes, adopt the technique of underpressure distillation, collect distillate, add distilled water and repeat to distill 1-2 time;
(5) merge distillate, by extracted with diethyl ether, and add desiccant dehydration;
(6) adopt heating in water bath to reclaim ether, obtain little Herba Schizonepetae volatile oil.
2. a kind of method of extracting volatile oil from little schizonepeta according to claim 1, is characterized in that: the amount that adds distilled water described in step (2) is 5-10 times of broken section of weight of little schizonepeta, and soak time is 20-40min.
3. a kind of method of extracting volatile oil from little schizonepeta according to claim 1, it is characterized in that: the biological enzyme described in step (3) is a kind of in cellulase, amylase, hemicellulase and polygalacturonase, Temperature Setting during enzymolysis is at 30-45 ℃, and enzymolysis time is 1-3 hour.
4. a kind of method of extracting volatile oil from little schizonepeta according to claim 1, is characterized in that: underpressure distillation described in step (4), temperature is controlled at 50-60 ℃.
5. a kind of method of extracting volatile oil from little schizonepeta according to claim 1, is characterized in that: the siccative described in step (5) is a kind of in anhydrous sodium sulphate, anhydrous magnesium sulfate.
6. a kind of method of extracting volatile oil from little schizonepeta according to claim 1, is characterized in that: the amount that adds ether described in step (5) is 2-5 times of distillate volume, and the siccative adding is the 1/5-1/4 of broken section of weight of little schizonepeta.
7. a kind of method of extracting volatile oil from little schizonepeta according to claim 1, is characterized in that: the bath temperature described in step (6) is controlled at 37-41 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410266373.3A CN104073350A (en) | 2014-06-16 | 2014-06-16 | Method for extracting volatile oil from small schizonepeta |
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| CN201410266373.3A CN104073350A (en) | 2014-06-16 | 2014-06-16 | Method for extracting volatile oil from small schizonepeta |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560400A (en) * | 2014-12-26 | 2015-04-29 | 江苏省中国科学院植物研究所 | Method for extracting peppermint oil |
| CN104719351A (en) * | 2015-02-05 | 2015-06-24 | 成都新朝阳作物科学有限公司 | Schizonepeta extractive and application thereof |
| CN104974853A (en) * | 2015-07-17 | 2015-10-14 | 王再青 | Method for extracting peppermint essential oil |
| CN105001993A (en) * | 2015-07-17 | 2015-10-28 | 王再青 | Method for increasing extraction ratio of Sandalwood essential oil |
| CN105062686A (en) * | 2015-07-17 | 2015-11-18 | 王再青 | Method for extracting lavender essential oil |
| CN105087166A (en) * | 2015-07-17 | 2015-11-25 | 王再青 | Method for extracting Jasminum sambac essential oil |
| CN105112163A (en) * | 2015-07-27 | 2015-12-02 | 湖北中烟工业有限责任公司 | Method for extracting volatile oil for cigarettes from schizonepeta leaves |
| CN105602733A (en) * | 2016-02-22 | 2016-05-25 | 广西梧州制药（集团）股份有限公司 | Method for auxiliary extraction of atractylodes volatile oil through enzymolysis method |
| CN107974350A (en) * | 2017-12-14 | 2018-05-01 | 北京国康本草物种生物科学技术研究院有限公司 | A kind of volatile argyi leaf oil and preparation method thereof |
| CN112715647A (en) * | 2020-12-29 | 2021-04-30 | 江西中医药大学 | Plant body coating pretreatment drying method and volatile oil extraction process |
| CN115109653A (en) * | 2022-07-22 | 2022-09-27 | 吉水县金海天然香料油科技有限公司 | Preparation method of schizonepeta volatile oil |
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2014
- 2014-06-16 CN CN201410266373.3A patent/CN104073350A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560400A (en) * | 2014-12-26 | 2015-04-29 | 江苏省中国科学院植物研究所 | Method for extracting peppermint oil |
| CN104719351A (en) * | 2015-02-05 | 2015-06-24 | 成都新朝阳作物科学有限公司 | Schizonepeta extractive and application thereof |
| CN104974853A (en) * | 2015-07-17 | 2015-10-14 | 王再青 | Method for extracting peppermint essential oil |
| CN105001993A (en) * | 2015-07-17 | 2015-10-28 | 王再青 | Method for increasing extraction ratio of Sandalwood essential oil |
| CN105062686A (en) * | 2015-07-17 | 2015-11-18 | 王再青 | Method for extracting lavender essential oil |
| CN105087166A (en) * | 2015-07-17 | 2015-11-25 | 王再青 | Method for extracting Jasminum sambac essential oil |
| CN105112163A (en) * | 2015-07-27 | 2015-12-02 | 湖北中烟工业有限责任公司 | Method for extracting volatile oil for cigarettes from schizonepeta leaves |
| CN105602733A (en) * | 2016-02-22 | 2016-05-25 | 广西梧州制药（集团）股份有限公司 | Method for auxiliary extraction of atractylodes volatile oil through enzymolysis method |
| CN107974350A (en) * | 2017-12-14 | 2018-05-01 | 北京国康本草物种生物科学技术研究院有限公司 | A kind of volatile argyi leaf oil and preparation method thereof |
| CN112715647A (en) * | 2020-12-29 | 2021-04-30 | 江西中医药大学 | Plant body coating pretreatment drying method and volatile oil extraction process |
| CN115109653A (en) * | 2022-07-22 | 2022-09-27 | 吉水县金海天然香料油科技有限公司 | Preparation method of schizonepeta volatile oil |
| CN115109653B (en) * | 2022-07-22 | 2024-01-16 | 吉水县金海天然香料油科技有限公司 | Preparation method of schizonepeta volatile oil |
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