CN104069494B - A kind of sodium alginate micro gel capsule for tumor microwave heat treatment technology and its preparation method and application - Google Patents
A kind of sodium alginate micro gel capsule for tumor microwave heat treatment technology and its preparation method and application Download PDFInfo
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- CN104069494B CN104069494B CN201410336454.6A CN201410336454A CN104069494B CN 104069494 B CN104069494 B CN 104069494B CN 201410336454 A CN201410336454 A CN 201410336454A CN 104069494 B CN104069494 B CN 104069494B
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Abstract
The present invention discloses a kind of sodium alginate micro gel capsule for tumor microwave heat treatment technology, and this microcapsule diameter is 1 10 μm, shell thickness 100 1000nm of microcapsule.First sodium alginate micro gel capsule is applied to tumor microwave heat treatment technical field in the present invention, and achieves good therapeutic effect, substantially increase diagnoses and treatment efficiency, there is good clinical value.
Description
Technical field
The present invention relates to microcapsule technology and its biologic applications field.More particularly, to a kind of for tumor microwave heat
The sodium alginate micro gel capsule for the treatment of technology and its preparation and application.
Background technology
At present, cancer have become as lead to one of principal disease of human death (Nanoscale, 2012,4,330-342,
Nanoscale,2013,5,12409-12424).According to World Health Organization (WHO)《World's cancer report in 2014》Display, 2012
Global cancer patient and death are all disturbingly increasing, and global cancer new cases reach more than 1,400 ten thousand, year by year
It is incremented to 19,000,000 people of 2025, be up to 24,000,000 people by 2035.Report also shows, Africa, Asia and Central and South America
Developing country's pathogenesis of cancer situation the severeest.The whole world increases 14,000,000 cases of cancers altogether newly and has 8,200,000 people within 2012
Dead.Wherein, China increases 3,070,000 cancer patients newly and causes about 2,200,000 people dead, accounts for 21.9% He of global total amount respectively
26.8%.
As can be seen here, cancer has seriously threatened life and the health of the mankind, brings huge bearing to society, family and individual
Load and misery.Conventional anti-cancer therapies are mainly carried out by chemotherapy, radiotherapy, operation etc., rather can bring necessarily to patient
Misery and side effect, therefore, develop a kind of extremely urgent (Nature of platform of thermotherapy cancer
reviews.Drug discovery,2010,615-27).In recent years, with the development of nanometer technique, it is that cancer diagnosis are controlled
Treat and bring new opportunity.
Thermotherapy has become as the effective emerging therapy of one kind of current cancer.Common external heat source includes swashing
Light, microwave, ultrasonic etc..Heat effect using microwave can carry out the thermotherapy of tumor.When microwave enters biological tissue, can produce
The transmission of microwave and the absorption of energy, thus form certain temperature rise in tissue.The physical basis of microwave heat therapeutic are electrolytes
There is dielectric loss in electromagnetic field, the heat being consumed distributes in media as well in the form of heat, so that the temperature of medium is raised.With
Other thermotherapy modes are compared, and the major advantage of microwave heat therapeutic is that the internal heating-up temperature of linked groups is higher, ablated volume is bigger, disappears
Time of melting is faster more uniform with heat flux distribution, and therefore it is considered as Therapeutic Method the most promising.Microwave heat therapeutic
Typically effectively kill temperature more than 4l～43 DEG C.
The United States Patent (USP) of Patent No. US20110034916A1, discloses a kind of magnetic that can be used for tumor microwave heat treatment
The preparation method and application of nano-particle.The step preparing above-mentioned magnetic nanoparticle includes：1) ferric acetyl acetonade is scattered in
In diphenyl ether, hexadecane, Oleic acid and oleyl amine, it is passed through nitrogen, is heated to 260 DEG C, flow back 30min；2) it is cooled to room temperature, separate and receive
Collection black magnetic nano-particle.Patent controlled can obtain the magnetic nanoparticle of 1～500nm size.The magnetic that patent obtains
Nano-particle is used for tumor microwave heat treatment, and obtains good therapeutic effect.Patent No. US005330518A and Patent No.
The United States Patent (USP) of US005861021A, also discloses that application in oncotherapy for the microwave heat therapeutic technology, and achieves good
Therapeutic effect.
Although microwave heat therapeutic technology has noinvasive or minimally invasive, the preferable feature of safety, its tremendous potential is not yet at present
It is fully developed and applies, main cause is that the research related to microwave heat therapeutic owes deeply such as to be used for the material of microwave enhanced sensitivity
Material selection is difficult to choose.It was used for the material own biological compatibility such as magnetic nanoparticle of thermotherapy in organism poor in the past, right
Organism has certain toxicity.
It is therefore desirable to develop good biocompatibility, the nontoxic microcapsule for microwave heat therapeutic technology.
Content of the invention
The invention solves the problems that first technical problem be provide a kind of sodium alginate for tumor microwave heat treatment technology
Microcapsule, this capsule has good biocompatibility, has no toxic side effect, and is used directly in animal body, is applied to
Tumor microwave heat treatment aspect, has good clinical value.
The invention solves the problems that second technical problem be provide for tumor microwave heat treatment technology sodium alginate micro gel
The preparation method of capsule, the microcapsule pattern of this method preparation is good, narrow diameter distribution, shell thickness, size tunable.
The invention solves the problems that the 3rd technical problem be provide application in tumor microwave heat treatment for the microcapsule.
For solving above-mentioned first technical problem, the present invention adopts following technical proposals：
A kind of sodium alginate micro gel capsule for tumor microwave heat treatment technology, described microcapsule diameter is 1-10 μm, described
The shell thickness 100-1000nm of microcapsule.
Preferably, described microcapsule diameter is 2-3 μm, the shell thickness 200-300nm of described microcapsule.
Preferably, the core of described microcapsule is inorganic salt, and the Shell Materials of described microcapsule are sodium alginate.
Preferably, described inorganic salt is selected from one or more of sodium chloride, potassium chloride, ammonium chloride and ammonium hydrogen carbonate.
For solving above-mentioned second technical problem, the present invention adopts following technical proposals：
For the preparation method of the sodium alginate micro gel capsule of tumor microwave heat treatment technology, comprise the steps：
1) sodium alginate is dissolved in water and obtains sodium alginate soln, add core material solution in sodium alginate soln, will mix
Close solution stirring uniform；
2) by step 1) the mixed solution emulsifying that obtains, solidification, form microcapsule wall；
To step 1) sequentially add oil phase and emulsifying agent, magnetic agitation 30 in the sodium alginate that obtains and core mixed solution
Minute, obtain homogeneous milky water-in-oil type (W/O) emulsion；Add calcium chloride as firming agent, magnetic agitation in W/O emulsion
30 minutes, form microcapsule wall；
3) by step 2) emulsion breaking containing molding microcapsule wall, separate, obtain sodium alginate micro gel capsule.
4) step 3) obtain a diameter of 1-10 μm of sodium alginate micro gel capsule, shell is thick to be 100-1000nm；Preferably,
Step 3) obtain a diameter of 2-3 μm of sodium alginate micro gel capsule, shell is thick to be 200-300nm.
Preferably, step 1) described sodium alginate soln concentration be 1-10wt%；Described core is selected from sodium chloride, chlorination
One or more of potassium, ammonium chloride and ammonium hydrogen carbonate；Described core is 1 with the mass ratio of sodium alginate:10-1:1.
Preferably, step 2) volume ratio of aqueous phase and oil phase of described emulsifying is 1:10-1:1；The emulsifying agent of described emulsifying
Volume fraction be 0.05-0.5%；Described emulsifying obtains homogeneous milky W/O emulsion it is preferable that described oil phase is selected from liquid
Paraffin or soybean salad oil.
Preferably, step 2) described firming agent be calcium chloride；Step 3) described demulsification demulsifier be selected from isopropanol and/
Or n-butyl alcohol.
Emulsifying agent used by the present invention is selected from Span80 or Tween20.
3rd technical problem to be solved by this invention is to provide sodium alginate micro gel capsule in tumor microwave heat treatment technology
In application, sodium alginate micro gel capsule is scattered in normal saline, local injection in knub position, microwave irradiation affected part, swell
Tumor position temperature can be rapidly reached more than 43 DEG C, and this temperature be enough to kill tumor cell.
Sodium alginate is a kind of natural polysaccharide, is the natural polysaccharide extracting from Brown algae or antibacterial, similar to extracellular
Glycosaminoglycans in substrate, no subacute/chronic toxicity or carcinogenecity reaction, also can be able to make as edible food additive
It is used for medical usage for timbering material, possesses good biocompatibility, there is stability needed for pharmaceutical preparation adjuvant, dissolving
Property, viscosity and safety.
Sodium chloride is a kind of safe and nontoxic life component, and sodium chloride is extremely important for tellurian life.Blood
Na ion concentration in liquid is directly connected to the regulation of the level of security of body fluid.The conduction of the neural impulse being led to by signal conversion
It is also to be adjusted by sodium ion.The aqueous solution of sodium chloride-containing 0.9% is referred to as normal saline, because it has identical to permeate with blood plasma
Pressure.Normal saline is main humor substitute, is widely used in treatment and prevention dehydration, is also used for intravenous medical treatment and prevention
Hypovolemic shock.The inorganic salt such as potassium chloride is also necessary to organism normal existence simultaneously, it may have comparatively ideal life
The thing compatibility.
Using sodium alginate as shell, the solution such as water core sodium chloride, potassium chloride, as core, prepares one kind and is used for
The sodium alginate micro gel capsule of tumor microwave heat treatment technology, described sodium alginate micro gel capsule preferably remains inorganic in core material solution
The microwave absorbing property of salt ion, because inorganic ion can occur function of ion polarization under microwave radiation, microwave direction
Frequently change can lead to ionic polarization direction frequently to change therewith, and interionic collides with each other, frictional heat, and microwave energy is direct
It is converted into heat energy, and then show in the form of temperature raises；Different inorganic ions are coated on alginic acid as core
After sodium microcapsule inside, due to the constraint effect of shell, the collision between ion under microwave radiation can be more violent, confinement
Effect makes microcapsule more microwave energys can be converted into heat under microwave radiation, and temperature rise effect can become apparent from.Will
Nontoxic sodium alginate micro gel capsule is applied to tumor microwave heat treatment aspect, has good clinical value.
Beneficial effects of the present invention are as follows：
1st, the present invention and prior art is dramatically different, is applied to swell by sodium alginate micro gel capsule first in the present invention
Tumor microwave heat therapeutic technical field, and achieve good therapeutic effect, substantially increase diagnoses and treatment efficiency, have and face well
Bed using value.
2nd, sodium alginate micro gel capsule-core material of the present invention is in sodium chloride, potassium chloride, ammonium chloride and ammonium hydrogen carbonate
The mixture of one or more, shell is sodium alginate, itself is respectively provided with good biocompatibility, secondary work nontoxic to organism
With.
3rd, the preparation method of the sodium alginate micro gel capsule for tumor microwave heat treatment technology of the present invention is simple, is not required to
Want special installation, reaction raw materials are easy to get, and cost ratio is relatively low, the sodium alginate micro gel capsule size uniformity of the method preparation, shell is relatively
Thickness, and dense, can effectively suppress release and the diffusion of core.Due to the meeting under microwave radiation of core inorganic ion
There is function of ion polarization, the frequent change in microwave direction can lead to ionic polarization direction frequently to change therewith, and interionic is mutual
Collision, frictional heat, microwave energy is converted into heat energy, and then shows in the form of temperature raises；By different nothings
After machine salt ion is coated on the intracapsular portion of sodium alginate micro gel as core, due to the constraint effect of shell, under microwave radiation
Collision between ion can be more violent, and confinement effect makes microcapsule can turn more microwave energys under microwave radiation
Turn to heat, temperature rise effect can become apparent from.Fig. 7 shows that sodium alginate micro gel capsule rises the advantage mechanism figure of warming middle-JIAO in microwave.With
When, because microcapsule itself has good biocompatibility, it is used directly for animal experiment in vivo.
Brief description
Below in conjunction with the accompanying drawings the specific embodiment of the present invention is described in further detail.
Fig. 1 illustrates the preparation method schematic diagram of the sodium alginate micro gel capsule for tumor microwave heat treatment technology；
Fig. 2 the embodiment of the present invention 1 is shown obtained by the sodium alginate micro gel capsule for tumor microwave heat treatment technology light
Learn microphotograph；
Fig. 3 the embodiment of the present invention 1 is shown obtained by the sodium alginate micro gel capsule for tumor microwave heat treatment technology grain
Footpath is distributed picture；
Fig. 4 the embodiment of the present invention 1 is shown obtained by the sweeping of the sodium alginate micro gel capsule for tumor microwave heat treatment technology
Retouch electron micrograph；
Fig. 5 illustrates the sodium alginate micro gel capsule surface for tumor microwave heat treatment technology obtained by the embodiment of the present invention 1
Electron scanning micrograph；
Fig. 6 illustrates the sodium alginate micro gel softgel shell layer for tumor microwave heat treatment technology obtained by the embodiment of the present invention 1
The electron scanning micrograph in section；
Fig. 7 the embodiment of the present invention 1 is shown obtained by the sodium alginate micro gel capsule for tumor microwave heat treatment technology micro-
Ripple rises the advantage mechanism figure of warming middle-JIAO；
Fig. 8 the embodiment of the present invention 1 is shown obtained by the sodium alginate micro gel capsule for tumor microwave heat treatment technology micro-
Ripple temperature rise effect.
Specific embodiment
In order to be illustrated more clearly that the present invention, with reference to preferred embodiments and drawings, the present invention is done further
Bright.In accompanying drawing, similar part is indicated with identical reference.It will be appreciated by those skilled in the art that institute is concrete below
The content of description is illustrative and be not restrictive, and should not be limited the scope of the invention with this.
Embodiment 1
(1) prepare core material solution：
Sodium alginate stirring and dissolving is obtained 1wt% sodium alginate soln in deionized water, adds in sodium alginate soln
Enter sodium chloride as core, sodium chloride is 1 with the mass ratio of sodium alginate:10, dispersed with stirring is uniform；
(2) emulsifying step, forms W/O emulsion：
Take the sodium alginate that step (1) obtains and sodium chloride mixed solution to conical flask, sequentially add liquid paraffin with
Emulsifying agent, aqueous phase and oil phase volume are than for 1:10, emulsifying agent used is Span80, and the volume fraction of emulsifying agent is 0.05%, magnetic
Power stirs 30 minutes, obtains homogeneous milky W/O emulsion；
(3) microcapsule wall curing schedule：
Add calcium chloride as firming agent, magnetic agitation 30 minutes in the W/O emulsion obtaining to step (2), formed stable
Microcapsule wall；
(4) demulsification, microcapsule separating step：
Add isopropanol to carry out breakdown of emulsion in the microcapsule emulsion obtaining to step (3), stop stirring reaction, outwell upper liquid
Body paraffin layer, microcapsule is dispersed in lower floor's aqueous phase, and aqueous phase is centrifuged, deionized water wash 3～5 times, obtains sea
Sodium alginate microcapsule, from Fig. 2,3 and 6 understand, the size of sodium alginate micro gel capsule manufactured in the present embodiment is 2-5 μm, shell
Thickness degree is 230nm, and Fig. 4 is the electron scanning micrograph of sodium alginate micro gel capsule manufactured in the present embodiment, and Fig. 5 is this reality
Apply the electron scanning micrograph on the sodium alginate micro gel capsule surface of example preparation.
The sodium alginate micro gel capsule that the present embodiment is prepared is used for tumor microwave heat treatment technology, and it is external and micro- in vivo
Ripple intensification experiment is as follows with tumor thermotherapy effect：
Sodium alginate micro gel capsule is scattered in normal saline, is placed in external microwave heating device, microwave irradiation is processed,
Intensification result is as shown in figure 8, respond well.The experiment that heats up in animal body is as follows：Sodium alginate micro gel capsule is scattered in physiology salt
In water, local injection is processed in the ICR mouse tumor position of inoculation H22 tumor, microwave irradiation, and knub position temperature can be rapid
Reach more than 43 DEG C, this temperature be enough to kill tumor cell；About two weeks, knub position returns to one's perfect health, and skin just recovers completely
Often.
Embodiment 2
(1) prepare core material solution：
Sodium alginate stirring and dissolving is obtained 10wt% sodium alginate soln in deionized water, adds in sodium alginate soln
Enter potassium chloride as core, potassium chloride is 1 with the mass ratio of sodium alginate:5, dispersed with stirring is uniform；
(2) emulsifying step, forms W/O emulsion：
Take the sodium alginate that step (1) obtains and potassium chloride mixed solution to conical flask, sequentially add liquid paraffin with
Emulsifying agent, aqueous phase is 1 with the volume ratio of oil phase:5, emulsifying agent used is Span80, and the volume fraction of emulsifying agent is 0.5%, magnetic
Power stirs 30 minutes, obtains homogeneous milky W/O emulsion；
(3) microcapsule wall curing schedule：
Add calcium chloride as firming agent, magnetic agitation 30 minutes in the W/O emulsion obtaining to step (2), formed stable
Microcapsule wall；
(4) demulsification, microcapsule separating step：
Add isopropanol to carry out breakdown of emulsion in the microcapsule emulsion obtaining to step (3), stop stirring reaction, outwell upper liquid
Body paraffin layer, microcapsule is dispersed in lower floor's aqueous phase, and aqueous phase is centrifuged, deionized water wash 3～5 times, obtains sea
Sodium alginate microcapsule, stand-by.
The sodium alginate micro gel capsule that the present embodiment is prepared is used for tumor microwave heat treatment technology, and it is external and micro- in vivo
Ripple intensification experiment is as follows with tumor thermotherapy effect：
Sodium alginate micro gel capsule is scattered in normal saline, is placed in external microwave heating device, microwave irradiation is processed,
Temperature rise effect is good.The experiment that heats up in animal body is as follows：Sodium alginate micro gel capsule is scattered in normal saline, local injection in
The ICR mouse tumor position of inoculation H22 tumor, microwave irradiation is processed, and knub position temperature can be rapidly reached more than 43 DEG C, should
Temperature be enough to kill tumor cell；About two weeks, knub position returns to one's perfect health, and skin recovers normal completely.
Embodiment 3
(1) prepare core material solution：
Sodium alginate stirring and dissolving is obtained 5wt% sodium alginate soln in deionized water, adds in sodium alginate soln
Enter ammonium chloride as core, ammonium chloride is 1 with the mass ratio of sodium alginate:10, dispersed with stirring is uniform；
(2) emulsifying step, forms W/O emulsion：
Take the sodium alginate that step (1) obtains and ammonium chloride mixed solution to conical flask, sequentially add liquid paraffin with
Emulsifying agent, aqueous phase is 1 with the volume ratio of oil phase:1, emulsifying agent used is Span80, and the volume fraction of emulsifying agent is 0.1%, magnetic
Power stirs 30 minutes, obtains homogeneous milky W/O emulsion；
(3) microcapsule wall curing schedule：
Add calcium chloride as firming agent, magnetic agitation 30 minutes in the W/O emulsion obtaining to step (2), formed stable
Microcapsule wall；
(4) demulsification, microcapsule separating step：
Add isopropanol to carry out breakdown of emulsion in the microcapsule emulsion obtaining to step (3), stop stirring reaction, outwell upper liquid
Body paraffin layer, microcapsule is dispersed in lower floor's aqueous phase, and aqueous phase is centrifuged, deionized water wash 3～5 times, obtains sea
Sodium alginate microcapsule, stand-by.
The sodium alginate micro gel capsule that the present embodiment is prepared is used for tumor microwave heat treatment technology, and it is external and micro- in vivo
Ripple intensification experiment is as follows with tumor thermotherapy effect：
Sodium alginate micro gel capsule is scattered in normal saline, is placed in external microwave heating device, microwave irradiation is processed,
Temperature rise effect is good.The experiment that heats up in animal body is as follows：Sodium alginate micro gel capsule is scattered in normal saline, local injection in
The ICR mouse tumor position of inoculation H22 tumor, microwave irradiation is processed, and knub position temperature can be rapidly reached more than 43 DEG C, should
Temperature be enough to kill tumor cell；About two weeks, knub position returns to one's perfect health, and skin recovers normal completely.
Embodiment 4
(1) prepare core material solution：
Sodium alginate stirring and dissolving is obtained 5wt% sodium alginate soln in deionized water, adds in sodium alginate soln
Enter ammonium hydrogen carbonate as core, ammonium hydrogen carbonate is 1 with the mass ratio of sodium alginate:5, dispersed with stirring is uniform；
(2) emulsifying step, forms W/O emulsion：
Take the sodium alginate that step (1) obtains and ammonium hydrogen carbonate mixed solution to conical flask, sequentially add liquid paraffin
With emulsifying agent, aqueous phase is 1 with the volume ratio of oil phase:5, emulsifying agent used is Span80, and the volume fraction of emulsifying agent is 0.2%,
Magnetic agitation 30 minutes, obtains homogeneous milky W/O emulsion；
(3) microcapsule wall curing schedule：
Add calcium chloride as firming agent, magnetic agitation 30 minutes in the W/O emulsion obtaining to step (2), formed stable
Microcapsule wall；
(4) demulsification, microcapsule separating step：
Add isopropanol to carry out breakdown of emulsion in the microcapsule emulsion obtaining to step (3), stop stirring reaction, outwell upper liquid
Body paraffin layer, microcapsule is dispersed in lower floor's aqueous phase, and aqueous phase is centrifuged, deionized water wash 3～5 times, obtains sea
Sodium alginate microcapsule, stand-by.
The sodium alginate micro gel capsule that the present embodiment is prepared is used for tumor microwave heat treatment technology, and it is external and micro- in vivo
Ripple intensification experiment is as follows with tumor thermotherapy effect：
Sodium alginate micro gel capsule is scattered in normal saline, is placed in external microwave heating device, microwave irradiation is processed,
Temperature rise effect is good.The experiment that heats up in animal body is as follows：Sodium alginate micro gel capsule is scattered in normal saline, local injection in
The ICR mouse tumor position of inoculation H22 tumor, microwave irradiation is processed, and knub position temperature can be rapidly reached more than 43 DEG C, should
Temperature be enough to kill tumor cell；About two weeks, knub position returns to one's perfect health, and skin recovers normal completely.
Embodiment 5～8
With embodiment 1～4, it the difference is that only, the oil phase liquid paraffin soybean salad oil generation that step (2) adds
Replace, result is similar.
Embodiment 9～16
With embodiment 1～8, it the difference is that only, the emulsifying agent Span80 that step (2) adds is replaced with Tween20,
Result is similar.
Embodiment 17～32,
With embodiment 1～16, it the difference is that only, the demulsifier isopropanol that step (4) adds is replaced with n-butyl alcohol,
Result is similar.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not right
The restriction of embodiments of the present invention, for those of ordinary skill in the field, also may be used on the basis of the above description
To make other changes in different forms, all of embodiment cannot be exhaustive here, every belong to this
Obvious change that bright technical scheme is extended out or change the row still in protection scope of the present invention.
Claims (8)
1. a kind of sodium alginate micro gel capsule for tumor microwave heat treatment technology it is characterised in that：Described microcapsule diameter is 1-
10 μm, the shell of described microcapsule is thick to be 100-1000nm；
The core of described microcapsule is inorganic salt, and the Shell Materials of described microcapsule are sodium alginate；
Described inorganic salt is selected from one of sodium chloride, potassium chloride, ammonium chloride or ammonium hydrogen carbonate.
2. microcapsule according to claim 1 it is characterised in that：Described microcapsule diameter is 2-3 μm, described microcapsule
Shell is thick to be 200-300nm.
3. it is used for the preparation method of the sodium alginate micro gel capsule of tumor microwave heat treatment technology, its feature as claimed in claim 1
It is, comprise the steps：
1) sodium alginate is dissolved in water and obtains sodium alginate soln, add core material solution in sodium alginate soln, will mix molten
Liquid stirs；
2) by step 1) the mixed solution emulsifying that obtains, solidification, form microcapsule wall；
3) by step 2) emulsion breaking containing molding microcapsule wall, separate, obtain sodium alginate micro gel capsule；
4) step 3) obtain a diameter of 1-10 μm of sodium alginate micro gel capsule, shell is thick to be 100-1000nm.
4. method according to claim 3 it is characterised in that：Step 3) a diameter of 2- of sodium alginate micro gel capsule of obtaining
3 μm, shell is thick to be 200-300nm.
5. method according to claim 3 it is characterised in that：Step 1) described sodium alginate soln concentration be 1-
10wt%；Described core is selected from one or more of sodium chloride, potassium chloride, ammonium chloride and ammonium hydrogen carbonate；Described core and sea
The mass ratio of sodium alginate is 1:10-1:1.
6. method according to claim 3 it is characterised in that：Step 2) aqueous phase of described emulsifying with the volume ratio of oil phase is
1:10-1:1；The volume fraction of emulsifying agent used by described emulsifying is 0.05-0.5%；Described emulsifying obtains homogeneous milky oil bag
Water emulsion.
7. method according to claim 6 it is characterised in that：Described oil phase is selected from liquid paraffin or soybean salad oil.
8. method according to claim 3 it is characterised in that：Step 2) described firming agent be calcium chloride；Step 3) described
The demulsifier of demulsification is selected from isopropanol and/or n-butyl alcohol.
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