CN104048957A - Glucose detection method based on peroxidase-like catalysis characteristics of graphene quantum dots - Google Patents
Glucose detection method based on peroxidase-like catalysis characteristics of graphene quantum dots Download PDFInfo
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Abstract
The invention discloses a glucose detection method based on peroxidase-like catalysis characteristics of graphene quantum dots, and belongs to the technical field of optical sensing. The graphene quantum dots have the peroxidase-like catalytic activity, can catalyze hydrogen peroxide reduction in the presence of hydrogen peroxide, also can oxidize colorless reduced-form tetramethyl benzidine into oxidized-form tetramethyl benzidine to show blue and produce an ultraviolet absorption peak at 652nm. In the presence of oxygen, glucose oxidase catalyzes oxidation of glucose to produce the hydrogen peroxide, the hydrogen peroxide reduction is catalyzed by use of the peroxidase-like catalysis characteristics of the graphene quantum dots, and the reduced-form tetramethyl benzidine in the solution is oxidized to show color. The glucose concentration is greater, the blue color of the solution is deeper, and the ultraviolet absorption strength is stronger, so that fast, sensitive and visual glucose detection can be realized.
Description
Technical field
A kind of glucose detection method that the present invention relates to class Peroxidative Characteristics based on graphene quantum dot, belongs to optical sensing technical field.
Background technology
Native enzyme is biocatalyst that exist in vivo, effective.Because they have very high biospecificity and catalytic activity, be applied to the fields such as medicine, biotechnology, chemical industry and environmental science.In these native enzyme, peroxidase, because can carry out various chemical reactions by catalyzing hydrogen peroxide, is widely used in the fields such as analyzing and diagnosing.Horseradish peroxidase is a kind of modal peroxidase, is often used in the detection of hydrogen peroxide and glucose.But, the impact that the catalytic activity of native enzyme is changed by the physical environment such as pH, temperature easily.In addition, the extraction of enzyme, purifying and carrying cost are also all higher.Therefore, study new, effectively, the bionical thing of peroxidase that can be used for practical application is significant.
Graphene quantum dot (GQD) is the graphene film that a kind of particle diameter is less than 100 nm, has good optics and characteristic electron, and scattered, fluorescence is strong, low toxicity, good biocompatibility.These character are widely used in bio-imaging, bio-sensing and photovoltaic device GQD.But, all design based on its surperficial carboxyl or electric charge about the research of GQD at present, about the research of GQD catalytic activity still less.
Summary of the invention
The object of the present invention is to provide a kind of glucose detection method of the class Peroxidative Characteristics based on graphene quantum dot.
The present invention is achieved like this, and glucose oxidase (GOx) and glucose solution are mixed, and utilizes GOx to produce hydrogen peroxide (H to the catalytic reaction of glucose
2o
2); Add again the mixed solution of GQD and reduced form tetramethyl benzidine (TMB), GQD catalysis H
2o
2reduce, meanwhile, TMB is oxidized to oxidized form TMB(oxTMB), solution, from the colourless blueness that becomes, produces ultraviolet absorption peak at 652 nm places; Concentration of glucose is larger, the H that GOx catalysis glucose produces
2o
2more, under the catalytic action of GQD, the oxTMB amount of generation is more, and the blueness of solution is darker, and absorbance increases, and has set up the glucose detection method of the class Peroxidative Characteristics based on GQD.In addition, the selectivity of GOx to glucose catalysis and GQD are to H
2o
2good catalytic activity, can realize sensitivity to glucose and selectivity and detect.
The present invention is by the following technical solutions:
(1) preparation of graphene oxide (GO): 0.5 g dag and 0.5 g sodium nitrate are joined in the concentrated sulphuric acid that 23 mL mass percentage concentration are 98%, under condition of ice bath, stir, slowly add again 3 g potassium permanganate, form brown pastel at 35 ° of C stirred in water bath to solution, add 40 mL ultrapure waters and continue and stir 30 minutes, add again after 100 mL ultrapure waters, dropwise splash into mass percentage concentration and be 30% hydrogen peroxide, until solution colour becomes glassy yellow; It is neutral product being filtered while hot and clean to filtrate with ultrapure water, and in vacuum drying chamber, 50 ° of C are dry, make graphene oxide;
(2) preparation of graphene film: graphene oxide is placed in to tubular furnace, under nitrogen protection, in 300 ° of C reductase 12s hour, is cooled to room temperature, make graphene film;
(3) preparation of GQD: the mass percentage concentration that 0.05 g graphene film is placed in to volume ratio 1:3 is respectively ultrasonic 17 hours of the mixed solution of 98% and 68% the concentrated sulphuric acid and red fuming nitric acid (RFNA), add 250 mL ultrapure waters dilutions, with the miillpore filter suction filtration of 0.22 μ m to till neutrality; Filter cake is dissolved in 20 mL ultrapure waters, with the pH of NaOH regulator solution be 8 left and right, solution is transferred in autoclave in 200 ° of C reactions 12 hours, cool to room temperature; The graphene film of the solution obtaining being removed to large volume with the filtering with microporous membrane of 0.22 μ m, the brown filtrate obtaining is GQD solution.
(4) glucose detection: GOx and glucose solution are mixed, and 37 ° of C reactions 30 minutes, GOx catalysis glucose response produced H
2o
2; Add again the mixed solution of GQD and TMB, under room temperature, react 1 hour, under the catalytic action of GQD, H
2o
2reduce, TMB is oxidized to oxTMB simultaneously, make solution by the colourless blueness that changes into, produce obvious ultraviolet absorption peak at 652 nm places; Concentration of glucose is larger, the H of generation
2o
2more, under the catalytic action of GQD, the oxTMB amount generating is more, the blueness of solution is darker, the absorbance at 652 nm places increases, degree and concentration of glucose that absorbance increases are linear within the scope of 1-100 μ M, and the detection of glucose is limited to 0.1 μ M, show that the sensor that the present invention sets up can be used for the quick and Sensitive Detection to glucose.
Technique effect of the present invention is: utilization of the present invention GOx catalysis glucose under oxygen exists produces H
2o
2, and then in conjunction with the catalysis characteristics of the class peroxidase of GQD, at catalysis H
2o
2in reduction time,, make TMB oxidation occur and develop the color, according to the increase of the intensification of solution colour and uv absorption intensity, can realize glucose fast, sensitivity and selectivity detect, have a good application prospect.
Brief description of the drawings
Fig. 1 is the glucose detection method schematic diagram of the class Peroxidative Characteristics based on GQD.
Fig. 2 is the Fourier transform infrared spectroscopy figure of (A) GO and GQD, (B) the ultraviolet-visible spectrogram of GQD and fluorescence spectrum figure (excitation wavelength is 310 nm), and interior illustration is the photo of GQD under radiation of visible light and ultra violet lamp.
Fig. 3 is the atomic force microscope figure of (A) GQD, and interior illustration is particle diameter distribution plan, (B) the transmission electron microscope figure of GQD, and interior illustration is particle diameter distribution plan.
Fig. 4 is the H of (A) variable concentrations
2o
2ultraviolet-visible spectrogram while existence, the H from a to o
2o
2concentration is 0,0.0012,0.0024,0.012,0.024,0.05,0.1,0.2,0.4,0.72,1.5,3,6,12,24 mM, (B) absorbance at 652 nm places and H successively
2o
2the linearity curve of concentration, interpolation photo is the H that has variable concentrations
2o
2time solution colour variation photo.
Fig. 5 is the ultraviolet-visible spectrogram of the glucose of (A) variable concentrations while existing, be followed successively by from a to l concentration of glucose is 0,0.001,0.002,0.005,0.01,0.02,0.05,0.1,0.2,0.5,1,2 mM, (B) 652 absorbances at nm place and the linearity curve of concentration of glucose, interpolation photo is the variation photo of solution colour while there is the glucose of variable concentrations.
Fig. 6 is to glucose, other kind sugar and the experiment of amino acid whose selectivity.
Embodiment
Below in conjunction with the drawings and specific embodiments, the present invention is further elaborated, but the present invention is not limited to this.
The preparation of graphene quantum dot:
(1) preparation of graphene oxide: 0.5 g dag and 0.5 g sodium nitrate are joined in the concentrated sulphuric acid that 23 mL mass percentage concentration are 98%, under condition of ice bath, stir, slowly add again 3 g potassium permanganate, form brown pastel at 35 ° of C stirred in water bath to solution, add 40 mL ultrapure waters and continue and stir 30 minutes, add again after 100 mL ultrapure waters, dropwise splash into mass percentage concentration and be 30% hydrogen peroxide, until solution colour becomes glassy yellow; It is neutral product being filtered while hot and clean to filtrate with ultrapure water, and in vacuum drying chamber, 50 ° of C are dry, make graphene oxide;
(2) preparation of graphene film: graphene oxide is placed in to tubular furnace, under nitrogen protection, in 300 ° of C reductase 12s hour, is cooled to room temperature, make graphene film;
(3) preparation of graphene quantum dot: the mass percentage concentration that 0.05 g graphene film is placed in to volume ratio 1:3 is respectively ultrasonic 17 hours of the mixed solution of 98% and 68% the concentrated sulphuric acid and red fuming nitric acid (RFNA), add 250 mL ultrapure waters dilutions, with the miillpore filter suction filtration of 0.22 μ m to till neutrality; Filter cake is dissolved in 20 mL ultrapure waters, with the pH of NaOH regulator solution be 8 left and right, solution is transferred in autoclave in 200 ° of C reactions 12 hours, cool to room temperature; The graphene film of the solution obtaining being removed to large volume with the filtering with microporous membrane of 0.22 μ m, the brown filtrate obtaining is graphene quantum dot solution.
Adopt Fourier transform infrared to characterize synthetic GO and GQD, result as shown in Figure 2 A.With GO(curve a) compared with, GQD(curve CO/COOH b) is at 1240 cm
-1the stretching vibration intensity at place obviously diminishes, and epoxide group in GQD is at 1052 cm
-1the shuttle belt at place almost disappears completely, shows that GQD is successfully synthetic.Fig. 2 B is that (a) (b), GQD has a very little absorption band at 310 nm places to curve to curve, and in the time that excitation wavelength is 310 nm, GQD has at 450 nm places the emission peak that fluorescence is very strong with fluorescence spectrum figure for the uv-visible absorption spectra figure of GQD.Interior illustration is the photo of GQD under visible ray and UV-irradiation, and GQD solution is weak yellow liquid under radiation of visible light, and under UV-irradiation, sends very strong blue light.Above result shows, adopts the inventive method to synthesize fluorescence GQD.
Fig. 3 is that atomic force microscope and the transmission electron microscope of GQD characterizes.From atomic force microscope figure, the sheet that GQD is evenly distributed, be highly 1.0 nm left and right (Fig. 3 A), and from transmission electron microscope picture, the diameter of GQD is 7-8 nm left and right, form is consistent with atomic force microscope result, further shows to adopt the inventive method, has made individual layer or double-deck GQD.
Class Peroxidative Characteristics based on GQD detects H
2o
2: detect principle as shown in Figure 1, the TMB of the GQD of 10 μ g/mL and 0.8 mM is mixed, add the H of variable concentrations
2o
2, under room temperature, to react after 1 hour and carry out ultraviolet spectroscopy, result is as shown in Figure 4.Work as H
2o
2concentration hour, along with H
2o
2the increase of concentration, the absorbance at 652 nm places rises rapidly; Work as H
2o
2when concentration increases to 6 mM, absorbance ascending velocity is slow gradually, until H
2o
2when concentration reaches 12 mM, absorbance no longer rises.Absorbance and H
2o
2concentration within the scope of 0.05-3 mM, be good linear relationship (illustration in Fig. 4 B), detect and be limited to 0.1 μ M.Fig. 4 B interpolation photo is with H
2o
2concentration increases, and the situation of change of solution colour, along with H
2o
2the increase of concentration, solution blueness is deepened gradually, can be used for H
2o
2quick visualization detect.
Class peroxidase activity based on GQD detects glucose: detect principle as shown in Figure 1, under oxygen exists, the glucose solution of GOx and variable concentrations is mixed, 37 ° of C reactions 30 minutes, add again the GQD of 10 μ g/mL and the TMB solution of 0.8 mM, under room temperature, react after 1 hour and carry out ultraviolet spectroscopy, result as shown in Figure 5.Along with the increase of concentration of glucose, the H that GOx catalysis glucose produces
2o
2more, under the catalytic action of GQD, the oxTMB amount of generation is more, and the blueness of solution is darker, and the absorbance at 652 nm places increases (Fig. 5 A); Degree and concentration of glucose linear within the scope of 1-100 μ M (Fig. 5 B) that absorbance increases, be limited to 0.1 μ M to the detection of glucose.Fig. 5 B interpolation photo is the situation of change that solution colour increases with concentration of glucose, and from photo, along with the increase of concentration of glucose, the color of solution deepens gradually, can be used for the quick visualization of glucose to detect.
In order to verify the selectivity of the inventive method to glucose detection, by the same way glucose is replaced with other sugar (as, fructose, sucrose, galactose, maltose) and amino acid (as, fruit methyllanthionine, proline, threonine, lysine), measurement result is as shown in Figure 6.Except glucose being had strong absorption, all very little to other sugar and amino acid whose absorbance, show that the method that the present invention builds has good selectivity to glucose detection, the selectivity that can be used for glucose in complex system detects.
Claims (3)
1. the glucose detection method of the class Peroxidative Characteristics based on graphene quantum dot, it is characterized in that the detection of glucose: glucose oxidase and glucose solution are mixed, 37 ° of C reactions 30 minutes, glucose oxidase enzymatic glucose response produced hydrogen peroxide; Add again the mixed solution of graphene quantum dot and reduced form tetramethyl benzidine, under room temperature, react 1 hour, under the catalytic action of graphene quantum dot, hydrogen peroxide reduces, reduced form tetramethyl benzidine is oxidized to oxidized form tetramethyl benzidine simultaneously, make solution by the colourless blueness that changes into, produce obvious ultraviolet absorption peak at 652 nm places; It is linear that the degree that absorbance increases and concentration of glucose are within the scope of 1-100 μ M, can be used for the quick and Sensitive Detection to glucose.
2. the glucose detection method of the class Peroxidative Characteristics based on graphene quantum dot according to claim 1, is characterized in that described graphene quantum dot preparation process is as follows:
(1) preparation of graphene oxide: 0.5 g dag and 0.5 g sodium nitrate are joined in the concentrated sulphuric acid that 23 mL mass percentage concentration are 98%, under condition of ice bath, stir, slowly add again 3 g potassium permanganate, form brown pastel at 35 ° of C stirred in water bath to solution, add 40 mL ultrapure waters and continue and stir 30 minutes, add again after 100 mL ultrapure waters, dropwise splash into mass percentage concentration and be 30% hydrogen peroxide, until solution colour becomes glassy yellow; It is neutral product being filtered while hot and clean to filtrate with ultrapure water, and in vacuum drying chamber, 50 ° of C are dry, make graphene oxide;
(2) preparation of graphene film: graphene oxide is placed in to tubular furnace, under nitrogen protection, in 300 ° of C reductase 12s hour, is cooled to room temperature, make graphene film;
(3) preparation of graphene quantum dot: 0.05 g graphene film is placed in to the concentrated sulphuric acid of volume ratio 1:3 and the mixed solution of red fuming nitric acid (RFNA) ultrasonic 17 hours, adds 250 mL ultrapure waters dilutions, with the miillpore filter suction filtration of 0.22 μ m to till neutrality; Filter cake is dissolved in 20 mL ultrapure waters, with the pH of NaOH regulator solution be 8 left and right, solution is transferred in autoclave in 200 ° of C reactions 12 hours, cool to room temperature; The graphene film of the solution obtaining being removed to large volume with the filtering with microporous membrane of 0.22 μ m, the brown filtrate obtaining is graphene quantum dot solution.
3. the glucose detection method of the class Peroxidative Characteristics based on graphene quantum dot according to claim 2, is characterized in that, in step (3), the mass percentage concentration of the described concentrated sulphuric acid is 98%, and the mass percentage concentration of red fuming nitric acid (RFNA) is 68%.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316503A (en) * | 2014-10-29 | 2015-01-28 | 安徽师范大学 | Use and detection method of sensor based on grapheme quantum dot (GQDs) |
| CN107101986A (en) * | 2017-06-13 | 2017-08-29 | 南昌大学 | A kind of blood coagulation enzyme assay method based on graphene quantum dot/erbium ion fluorescent nano probe |
| CN107179342A (en) * | 2017-05-18 | 2017-09-19 | 山东理工大学 | A kind of preparation method and application of the Serum Markers of Hepatitis B Virus immunosensor based on GQDs CuO@3D rGO |
| CN107561070A (en) * | 2017-08-28 | 2018-01-09 | 菏泽学院 | A kind of glucose quick visualization detection method and its preparation and application |
| CN110609032A (en) * | 2019-09-03 | 2019-12-24 | 东华理工大学 | Glucose detection method based on application of molybdenum oxide quantum dots as peroxide mimic enzyme |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507693A (en) * | 2011-11-03 | 2012-06-20 | 桂林医学院 | Functional-material-based glucose biosensor and manufacturing method thereof |
| CN102807209A (en) * | 2012-08-02 | 2012-12-05 | 清华大学 | Method for preparing graphene quantum dots |
| CN103512878A (en) * | 2013-08-16 | 2014-01-15 | 南昌大学 | Sensor preparation method based on ECL-RET action between GO and GQDs and application on kinas detection |
-
2014
- 2014-05-14 CN CN201410202591.0A patent/CN104048957B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507693A (en) * | 2011-11-03 | 2012-06-20 | 桂林医学院 | Functional-material-based glucose biosensor and manufacturing method thereof |
| CN102807209A (en) * | 2012-08-02 | 2012-12-05 | 清华大学 | Method for preparing graphene quantum dots |
| CN103512878A (en) * | 2013-08-16 | 2014-01-15 | 南昌大学 | Sensor preparation method based on ECL-RET action between GO and GQDs and application on kinas detection |
Non-Patent Citations (2)
| Title |
|---|
| AI-XIAN ZHENG ET AL.: "Highly-efficient peroxidase-like catalytic activity of graphene dots for biosensing", 《BIOSENSORS ANDBIOELECTRONICS》 * |
| 冯亚强等: "石墨烯量子点的制备、表征及应用研究", 《中国优秀硕士论文全文数据库 工程科技I辑》 * |
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| CN107561070A (en) * | 2017-08-28 | 2018-01-09 | 菏泽学院 | A kind of glucose quick visualization detection method and its preparation and application |
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| CN110779914A (en) * | 2019-11-05 | 2020-02-11 | 湖南科技大学 | Preparation method of kit based on hemoglobin and four-carbon or five-carbon dicarboxylic acid compound |
| CN110907589A (en) * | 2019-11-21 | 2020-03-24 | 福建师范大学福清分校 | Visible Cu detection based on GQDs photocatalysis2+Method (2) |
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