CN104045675A - Process for extracting tylosin - Google Patents
Process for extracting tylosin Download PDFInfo
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- CN104045675A CN104045675A CN201410283977.9A CN201410283977A CN104045675A CN 104045675 A CN104045675 A CN 104045675A CN 201410283977 A CN201410283977 A CN 201410283977A CN 104045675 A CN104045675 A CN 104045675A
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- filtrate
- molecular weight
- extraction process
- nanofiltration
- tylosin
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Abstract
The invention relates to a process for extracting tylosin. The process comprises the following steps of (s1) filtering fermentation broth, and filtering fermentation filtrate by an ultrafiltration membrane to remove soluble impurities with relatively large molecular weight from the filtrate; (s2) concentrating ultra-filtered filtrate by a nanofiltration membrane to remove water, salts and other impurities; and (s3) extracting nano-filtered filtrate with butyl acetate. According to the process disclosed by the invention, since soluble impurities with relatively large molecular weight are removed by the ultrafiltration membrane, the amount of impurities in the extraction process is effectively reduced, and the quality of the product is improved; the concentration is carried out by the nanofiltration membrane, the amount of butyl acetate in the extraction process is reduced, the production cost is saved and the potential safety hazard and environmental pollution are reduced.
Description
Technical field
The present invention relates to a kind of new tylosin extraction process.
Background technology
Tylosin is that fowl is raiseeed special-purpose microbiotic, and this microbiotic is the product of streptomyces fradiae fermentation.Traditional technology generally adopts liquid submerged fermentation, after fermented liquid adds flocculation agent (as Tai-Ace S 150 or aluminum chloride) to process, through solid-liquid separation, ferment filtrate extracts through organic solvent (as butylacetate) again, and ester phase liquid makes through treating processes such as dehydration, salifies again.This tylosin extraction process directly extracts the filtrate obtaining after filtering fermentation liquor with butylacetate; macromole impurity in technique filtrate (mainly comprising the soluble large molecule materials such as high molecular weight protein and fiber) cannot be removed in advance the production quality control of follow-up operation is brought to impact; the butylacetate large usage quantity of simultaneously using; cause the labour protection difficulty in workshop large; workshop turnover butylacetate amount is large, causes butylacetate consumption large.
Summary of the invention
For overcoming above-mentioned shortcoming, the object of this invention is to provide a kind of new tylosin extraction process, not only can improve the quality of products, while reducing extraction, the consumption of butylacetate, alleviates the pollution to environment simultaneously.
For solving the problems of the technologies described above, the present invention adopts following scheme to realize:
Tylosin extraction process, is characterized in that, mainly comprises the following steps:
(s1) after filtering fermentation liquor, use ultrafiltration membrance filter ferment filtrate, remove the larger soluble impurity of molecular weight in filtrate;
(s2) filtrate after using nanofiltration membrane to ultrafiltration concentrates, and removes the impurity such as moisture, inorganic salt;
(s3) filtrate after nanofiltration is extracted with butylacetate.
Wherein, ultra-filtration membrane described in step (s1) can see through molecular weight and be not more than 20000 daltonian macromolecular substance.
Wherein, in step (s2), nanofiltration membrane can see through molecular weight and be less than 200 daltonian small-molecule substances.
Wherein, after step (s3), also comprise successively reextraction, decolouring precipitation, rear nanofiltration, spray dried drying process.
Beneficial effect:
The one, by the larger soluble impurity of ultrafiltration membrance filter molecular weight before extraction, effectively reduced the amount of impurity in leaching process, improved quality product;
The 2nd, by nanofiltration membrane, concentrate, reduced the consumption of butylacetate in leaching process, save production cost, reduce potential safety hazard, reduce environmental pollution.
Accompanying drawing explanation
Fig. 1 is the schema of tylosin extraction process of the present invention.
Embodiment
By describing technology contents of the present invention, structural attitude in detail, being realized object and effect, below in conjunction with embodiment and coordinate accompanying drawing to be explained in detail.
Referring to Fig. 1, is the schema of tylosin extraction process of the present invention.Tylosin extraction process, mainly comprises the following steps:
(s1) after filtering fermentation liquor, use ultrafiltration membrance filter ferment filtrate, remove the larger soluble impurity of molecular weight in filtrate, ultra-filtration membrane can be greater than 20000 daltonian macromolecular substance by molecular weight cut-off here, thereby can improve filtrate quality;
(s2) filtrate after using nanofiltration membrane to ultrafiltration after ultrafiltration concentrates, remove the impurity such as water, inorganic salt, reduce the volume of filtrate, here nanofiltration membrane is that molecular weight is less than the permeable filtration membrane of 200 daltonian small-molecule substance, water in filtrate sees through nanofiltration membrane, and tylosin cannot see through nanofiltration membrane, and after nanofiltration, in filtrate, water will reduce more than 80%, filtrate can be concentrated into 5-6 doubly, thus the consumption of butylacetate while reducing follow-up n-butyl acetate extraction.
(s3) filtrate after nanofiltration is extracted with butylacetate.
After step (s3), also comprise successively that reextraction, decolouring precipitation, rear nanofiltration, spraying are dried.
Tylosin fermented liquid obtains filtrate after sheet frame or rotary drum filtration, this filtrate is after ultrafiltration and nanofiltration, by the nanofiltration concentrated solution obtaining and butylacetate under certain temperature and alkaline environment according to volume ratio 1:(1-2) extract the 50-80% left and right that now consumption of butylacetate is old technology.After extraction finishes, with tartrate or phosphoric acid solution, strip at a certain temperature.The filtrate that reextraction obtains adds medicinal carbon to decolour after regulating Ph value, and the destainer obtaining carries out stripping with uncontaminated air to residual a small amount of solvent.The filtrate finally obtaining through after nanofiltration and the dry tylosin pulvis that needs of can obtaining of spraying.
The present invention, by the larger soluble impurity of ultrafiltration membrance filter molecular weight before extraction, has effectively reduced the amount of impurity in leaching process, has improved quality product; By nanofiltration membrane, concentrate, reduced the consumption of butylacetate in leaching process, save production cost, reduce potential safety hazard, reduce environmental pollution.
These are only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes specification sheets of the present invention and accompanying drawing content to do; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (4)
1. tylosin extraction process, is characterized in that, mainly comprises the following steps:
(s1) after filtering fermentation liquor, use ultrafiltration membrance filter ferment filtrate, remove the larger soluble impurity of molecular weight in filtrate;
(s2) filtrate after using nanofiltration membrane to ultrafiltration concentrates, and removes the impurity such as moisture, inorganic salt;
(s3) filtrate after nanofiltration is extracted with butylacetate.
2. tylosin extraction process according to claim 1, is characterized in that, ultra-filtration membrane described in step (s1) can see through molecular weight and be not more than 20000 daltonian macromolecular substance.
3. tylosin extraction process according to claim 1, is characterized in that, in step (s2), nanofiltration membrane can see through molecular weight and be less than 200 daltonian small-molecule substances.
4. tylosin extraction process according to claim 1, is characterized in that, also comprises successively reextraction, decolouring precipitation, rear nanofiltration, spray dried drying process after step (s3).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410283977.9A CN104045675A (en) | 2014-06-24 | 2014-06-24 | Process for extracting tylosin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410283977.9A CN104045675A (en) | 2014-06-24 | 2014-06-24 | Process for extracting tylosin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104045675A true CN104045675A (en) | 2014-09-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410283977.9A Pending CN104045675A (en) | 2014-06-24 | 2014-06-24 | Process for extracting tylosin |
Country Status (1)
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| CN (1) | CN104045675A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107837682A (en) * | 2017-12-25 | 2018-03-27 | 南京九思高科技有限公司 | The device and method of drug products in a kind of bi-membrane method extraction organic solution |
| CN111620919A (en) * | 2020-06-05 | 2020-09-04 | 宁夏泰益欣生物科技有限公司 | Decoloration method of tylosin tartrate |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4362881A (en) * | 1980-07-02 | 1982-12-07 | Eli Lilly And Company | Tylactone |
| CN1374406A (en) * | 2000-09-30 | 2002-10-16 | 徐月清 | Extraction process of tylan |
| WO2003043642A1 (en) * | 2001-11-15 | 2003-05-30 | Enanta Pharmaceuticals Inc. | Novel tylosin derivatives having antibacterial activity |
| CN1432575A (en) * | 2002-01-14 | 2003-07-30 | 徐兵 | Tylan purifying process |
| CN101565438A (en) * | 2008-04-21 | 2009-10-28 | 王玉万 | Purification method for Tylosin |
| CN102746354A (en) * | 2012-07-17 | 2012-10-24 | 宁夏泰瑞制药股份有限公司 | Method for extracting tylosin by tylosin fermentation broth |
-
2014
- 2014-06-24 CN CN201410283977.9A patent/CN104045675A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4362881A (en) * | 1980-07-02 | 1982-12-07 | Eli Lilly And Company | Tylactone |
| CN1374406A (en) * | 2000-09-30 | 2002-10-16 | 徐月清 | Extraction process of tylan |
| WO2003043642A1 (en) * | 2001-11-15 | 2003-05-30 | Enanta Pharmaceuticals Inc. | Novel tylosin derivatives having antibacterial activity |
| CN1432575A (en) * | 2002-01-14 | 2003-07-30 | 徐兵 | Tylan purifying process |
| CN101565438A (en) * | 2008-04-21 | 2009-10-28 | 王玉万 | Purification method for Tylosin |
| CN102746354A (en) * | 2012-07-17 | 2012-10-24 | 宁夏泰瑞制药股份有限公司 | Method for extracting tylosin by tylosin fermentation broth |
Non-Patent Citations (1)
| Title |
|---|
| 王枢，等: "应用膜分离技术改进泰乐菌素提取工艺", 《食品与发酵工业》, vol. 37, no. 4, 30 April 2011 (2011-04-30) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107837682A (en) * | 2017-12-25 | 2018-03-27 | 南京九思高科技有限公司 | The device and method of drug products in a kind of bi-membrane method extraction organic solution |
| CN107837682B (en) * | 2017-12-25 | 2024-04-12 | 江苏久膜高科技股份有限公司 | Device and method for extracting medicine products in organic solution by double-membrane method |
| CN111620919A (en) * | 2020-06-05 | 2020-09-04 | 宁夏泰益欣生物科技有限公司 | Decoloration method of tylosin tartrate |
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Application publication date: 20140917 |