CN104043379B - The preparation method of a kind of agar/glucan composite gel microsphere - Google Patents
The preparation method of a kind of agar/glucan composite gel microsphere Download PDFInfo
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- CN104043379B CN104043379B CN201410281508.3A CN201410281508A CN104043379B CN 104043379 B CN104043379 B CN 104043379B CN 201410281508 A CN201410281508 A CN 201410281508A CN 104043379 B CN104043379 B CN 104043379B
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Abstract
The invention discloses the preparation method of a kind of agar/glucan composite gel microsphere.The method is hydroxyl reaction by the hydroxyl on agar gel microspheres and glucan and obtains composite gel microsphere.This complex microsphere mechanical strength is high, good stability, good biocompatibility, and with low cost, can be used as separating medium and bioactive substance carrier, is particularly with a wide range of applications in separation and purification.
Description
Technical field:
The present invention relates to bioengineering downstream, the preparation field of separation and purification medium.In particular, the present invention relates to the preparation method of a kind of agar-glucan composite gel microsphere.
Background technology:
In biological downstream processes, chromatography technology has the irreplaceable status of other separation means, and the key of chromatographic technique development is the exploitation of separating medium.In the large molecule separation and purification of Large Scale Biology, be low cost, high flow rate and better separating effect to the requirement of separating medium.
Ago-Gel is a kind of very conventional natural polysaecharides medium, has good compatibility with large biological molecule.The raw material agarose of Ago-Gel is extracted from agar, and high extraction cost makes the expensive of Ago-Gel, makes it be restricted in heavy industrialization application.
Agar is primarily of agarose and agaropectin composition.Agar gel microspheres is similar to agarose gel microsphere, has high-hydrophilic, macroporosity, highly porous, the feature of good biocompatibility, with the hydroxyl that can react with other chemical groups in a large number, and production cost is very low, is suitable as separating medium and uses on a large scale.The structure of agar gel is formed by interaction of hydrogen bond, the microballoon bad mechanical strength that this non-covalent bond is formed, and cannot realize the requirement of quick separating.The approach improving agar gel microspheres intensity has two, the first passes through chemical crosslinking, namely gel strength is improved by introducing covalent bond between the hydroxyl on polysaccharide chain, it two is agar contents by improving in gel micro-ball, but agar content increase makes solution viscosity increase, ball processed is more difficult, so the increase of agar content is limited, so usual use approach one.And the aperture of agar microballoon is comparatively large, is difficult to control, and with a small amount of negative electrical charge, can specific adsorption be caused.
Glucan, also known as dextran, is the straight-chain polysaccharide being polymerized by numerous dextran molecule and being formed.It has higher molecular weight, according to its monose number, usually can be divided into glucan 100,000, bextran 45 ten thousand, the polymer of series such as glucan 20,000 grade.Define cross-linked structure in crosslinked dextran molecule, can be used as molecular sieve, for the separation of the compound of different molecular weight, also can be used for desalination, displacement buffer solution etc.Containing a large amount of hydrophilic hydroxyl on the strand of glucan, be easy to carry out chemical modification, and be easy to control aperture by the amount of crosslinking agent and glucan.But the market price of sephadex costly at present, as glucan G25 price 1500-2500 unit.
The pluses and minuses of comprehensive agar gel microspheres and sephadex microsphere, we have developed a kind of agar-glucan composite gel microsphere.By chemical crosslinking, between hydroxyl, introduce covalent bond improve gel strength, glucan masks agar gel surface charge, and convenient control aperture.This cheap, aperture is easily controlled, and mechanical strength is good, and uncharged microballoon is suitable as separating medium and bioactive substance carrier, is with a wide range of applications.
Summary of the invention:
The object of the present invention is to provide a kind of agar/glucan composite gel microsphere and preparation method thereof.
The preparation method of a kind of agar/glucan composite gel microsphere, comprise: steps A, in reaction vessel, add agar gel microspheres and the dextran solution of same volume, in described agar gel microspheres, the content of agar is 2 ~ 20wt%, and the concentration of dextran solution is 0.05 ~ 1.5g/ml; Step B, at 25 ~ 55 DEG C, rotating speed is 60 ~ 200rpm, adds anhydrous sodium sulfate, after dissolving, then adds NaBH
4; Add sodium sulphate and NaBH
4containing 0.1 ~ 0.25g/mlNa in rear system
2sO
4with 0.008 ~ 0.02g/mlNaBH
4; Step C, in the basic conditions, adds double-functional group crosslinking agent; Step D, reaction temperature is 40 ~ 120 degrees Celsius, and duration of the reaction is 15 ~ 25h.
Further, in the agar gel microspheres described in steps A, the content of agar is 6 ~ 12wt%, and particle diameter is at 30 ~ 500 microns.
Further, the molecular weight of glucan is 2 ~ 200,000.
Further, in step C, dripping concentration is the NaOH solution of 20 ~ 70wt%, and its addition is 0.1 ~ 5 times of agar gel microspheres volume.
Further, the addition of crosslinking agent is 0.2 ~ 5 times of agar gel microspheres volume.
Further, crosslinking agent is epoxychloropropane, BDO bisglycidyl ether or pentaerythrite glycidol ether.
Agar-the application of glucan composite gel microsphere in abstraction and purification prepared by described method.
Technical scheme of the present invention is with the agar gel microspheres of variable concentrations agar content for matrix, and by crosslinked method, it is good to synthesize a kind of mechanical strength, and stability is high, good biocompatibility, and agar with low cost/glucan composite gel microsphere.
Accompanying drawing illustrates:
Fig. 1 is the optical microscope photograph of the agar microballoon of 6% after the once screening of preparation in embodiment 1.
Fig. 2 is the grading curve of the agar microballoon of 6% after the once screening of preparation in embodiment 1.
Fig. 3 is the scanning electron microscope (SEM) photograph of the agar microballoon of 6% after the once screening of preparation in embodiment 1.
Fig. 4 is the optical microscope photograph of the agar-glucan composite gel microsphere of preparation in embodiment 1.
Fig. 5 is the grading curve of the agar-glucan composite gel microsphere of preparation in embodiment 1.
Fig. 6 is the scanning electron microscope (SEM) photograph of the agar-glucan composite gel microsphere of preparation in embodiment 1.
Fig. 7 is the pressure-current curve figure of the agar-glucan composite gel microsphere of preparation in embodiment 1.
Fig. 8 is the separating effect figure of microballoon agar-glucan composite gel microsphere for saliferous bovine serum albumen solution of preparation in embodiment 1.
Fig. 9 is the separating effect figure of microballoon agar-glucan composite gel microsphere for saliferous ovalbumin solution of preparation in embodiment 1.
Figure 10 is the separating effect figure of microballoon agar-glucan composite gel microsphere for the meter He Genmao enzyme solutions of saliferous and little peptide of preparation in embodiment 1.
Figure 11 is the separating effect figure of microballoon agar-glucan composite gel microsphere for saliferous myoglobin solution of preparation in embodiment 1.
Figure 12 is the scanning electron microscope (SEM) photograph of the agar-glucan composite gel microsphere of preparation in embodiment 4.
Figure 13 is the scanning electron microscope (SEM) photograph of the agar-glucan composite gel microsphere of preparation in embodiment 5.
Figure 14 is the separating effect figure of agar-glucan composite gel microsphere for the mixed solution containing bovine serum albumin and glutathione of preparation in embodiment 5.
Figure 15 is the scanning electron microscope (SEM) photograph of the agar-glucan composite gel microsphere of preparation in embodiment 6.
Figure 16 is the separating effect figure of microballoon agar-glucan composite gel microsphere for saliferous bovine serum albumen solution of preparation in embodiment 7.
Figure 17 is the separating effect figure of microballoon agar-glucan composite gel microsphere for saliferous bovine serum albumen solution of preparation in embodiment 8.
Detailed description of the invention:
The preparation method of agar described in examples of implementation/glucan composite gel microsphere comprises the following steps: steps A, prepares agar gel microspheres by emulsification-solidification method, and in its preparation process, in aqueous phase, the content of agar is 2 ~ 20wt%; (patent before: Tan Tianwei, Hu Yu, Yang Zixin, Wei Jun, Chen Yanmin. a kind of preparation method of agar gel microspheres. the patent No. 201110220322.3), step one: make aqueous phase W by water-soluble for agar, wherein in aqueous phase, the content of agar is 4% ~ 20wt%; Step 2: oil soluble surfactant is dissolved in and makes oil phase O with water in mutual exclusive organic solvent, wherein in oil phase, containing volume ratio is the oil soluble surfactant of 10 ~ 20%; Step 3, aqueous phase is added oil phase and carry out dispersion and emulsion and obtain w/o type emulsion droplet, wherein the volume ratio of oil phase and aqueous phase is 1:1 ~ 10:1; Step 4, w/o type emulsion droplet obtains agar gel microspheres through cooling curing.
In reaction vessel, add 50ml agar microballoon and 50ml dextran solution, dextran solution concentration is 0.05 ~ 1.5g/ml; Step B, at 25 ~ 55 DEG C, rotating speed is 60 ~ 200rpm, adds anhydrous sodium sulfate, after dissolving, then adds NaBH
4; Step C, in the basic conditions, adds crosslinking agent; Step D, reaction temperature is 40 ~ 120 degrees Celsius, and duration of the reaction is 15 ~ 25h.
In a preferred embodiment of the invention, the agar of the agar microballoon that steps A is used is 2 ~ 20wt% in aqueous phase content, and be preferably 6-12wt%, the molecular weight of glucan is preferably 2 ~ 200,000, and the concentration of dextran solution is preferably 0.05 ~ 1.5g/ml.Reaction temperature in step B is 25 ~ 55 DEG C, and rotating speed is 60 ~ 200rpm, and in order to prevent agar variable color and hydrolysis in course of reaction, reaction is at 0.1 ~ 0.25g/mlNaSO
4with 0.008 ~ 0.02g/mlNaBH
4under existence condition through row.In step C, the NaOH solution of alkaline environment to be concentration be 20 ~ 70wt%, its addition is 0.1 ~ 5 times of agar gel microspheres volume, is preferably 0.4 ~ 3 times of agar gel microspheres volume.The addition of crosslinking agent is 0.2 ~ 5 times of agar microsphere volume, be preferably 0.4 ~ 3 times of agar gel microspheres volume, its crosslinking agent is the length crosslinking agent containing difunctional such as epoxychloropropane, BDO two bisglycidyl ether or pentaerythrite glycidol ether.In step D, temperature is 40 ~ 120 degrees Celsius, and duration of the reaction is 15 ~ 25h.Stop reaction, cleaning, to neutral, is preserved.Describe the present invention in detail below in conjunction with embodiment and accompanying drawing, these embodiments and accompanying drawing only play illustrative effect, are not limited to range of application of the present invention.
If do not have specified otherwise, all particle diameter units in the present invention are micron, and concentration unit is wt%, and temperature unit is DEG C.
Complex microsphere provided by the invention and preparation method thereof can also bring lower column effect:
Preparation method provided by the invention can prepare the gel micro-ball as bio-chemistry separation medium, for separation and the desalination of the material such as albumen, nucleic acid of different size size, can also as the carrier such as living cells, gene, for cell proliferation and the result for the treatment of played in body provide favourable micro.
Embodiment 1:
The agar gel microspheres of preparation 6wt%, cleans up, once sieves out the microballoon of 60 microns-150 microns, as for subsequent use in 20wt% ethanol.Adopt OLYMPUSCX41 (Olympus Co., Ltd, Japan) observation by light microscope product form, result as shown in Figure 1.Adopt MASTERSIZER2000 laser particle size analyzer (Malvern instrument company, Britain) analytic product granularity, result as shown in Figure 2.Take a morsel freeze drying 18h, and with product after scanning electron microscopic observation freeze-drying, result as shown in Figure 3.As can be seen from Figure 1, microballoon sphericity is good, and can obtain microspherulite diameter distribution homogeneous from Fig. 2, average grain diameter is 77.487 μm.As can be seen from Figure 3, the bad mechanical strength of microballoon, after freeze drying, microballoon is out of shape.
In 250mL there-necked flask, add the agar gel microspheres of the 6wt% that 50ml is sieved, 5g molecular weight be 40,000 glucan and 50mL deionized water, 35 DEG C, add 7.8g anhydrous sodium sulfate under 140rpm condition after, stirring and dissolving 0.5 hour, adds 0.6gNaBH
42mL50wt%NaOH solution, epoxychloropropane and 50wt%NaOH is added respectively again with the speed of 0.1mL/min, continue 6h, bath temperature is risen to 50 DEG C, Keep agitation 20 hours, be cooled to room temperature, adjust pH to neutral with acetic acid, after alternately cleaning with deionized water and 50wt% ethanol, the microballoon of gained is kept in 20wt% ethanol.Observation by light microscope product form, result as shown in Figure 4.MASTERSIZER2000 laser particle size analyzer (Malvern instrument company, Britain) analytic product granularity, result as shown in Figure 5.Take a morsel freeze drying 18h, and with product after scanning electron microscopic observation freeze-drying, result as shown in Figure 6.As can be seen from Figure 4, complex microsphere still maintains good sphericity, and can obtain microballoon distribution homogeneous from Fig. 5, average grain diameter is 83.726 μm, comparison diagram 2, and the average grain diameter that can obtain complex microsphere becomes large, should be in compound glucan just.As can be seen from Figure 3, after freeze drying, microballoon is out of shape, but compares with Fig. 3, and sphericity increases, and confirms that the mechanical strength of complex microsphere increases.Do the elementary analysis of microsphere surface, result display agar microsphere surface S element is 0.15wt%, and complex microsphere surface S element is 0wt%, shows that the negative electrical charge that agar surface contains S element is covered.
Embodiment 2:
Complex microsphere in embodiment 1 is loaded the gel chromatographic columns of 10*200mm, investigate the relation of its pressure and flow velocity, as shown in Figure 7.When flow velocity is increased to 3ml/min from 0.1ml/min, post pressure increase only about 0.1mpa, and when flow velocity is increased to 3.5ml/min, column pressure increases about 0.4mpa, illustrates that the post pressure that complex microsphere produces in separation process is less, is conducive to industrial applications.
Embodiment 3:
Desalination
By the complex microsphere dress post in embodiment 1 in the gel chromatographic columns of 10*200mm, sample is the saliferous bovine serum albumen solution, ovalbumin solution, meter He Genmao enzyme solutions, the trypsin solution that are dissolved in tris-hcl, eluent is the tris-hcl solution of ph7.8, and loading elution speed is 1ml/min.Microballoon respectively for the desalting effect of saliferous bovine serum albumin (66kda) solution, ovalbumin (45kda) solution, meter He Genmao enzyme (31kda) solution, myoglobins (16700da) solution as Fig. 8, Fig. 9, Figure 10, Figure 11.Containing some little peptide molecule and salting liquids in the meter He Genmao enzyme solutions of wherein Figure 10.The application that complex microsphere can realize desalination and separation can be obtained from Fig. 8 ~ Figure 11.
Embodiment 4
Add epoxychloropropane and 50%NaOH from different being of embodiment 1 respectively with the speed of 0.1mL/min, the duration changes 12h into by 6h.The dry 18h of freeze-drying, with product after scanning electron microscopic observation freeze-drying, result as shown in figure 12.Compared with Fig. 6, sphericity increases.
Embodiment 5
Add epoxychloropropane and 50wt%NaOH from different being of embodiment 1 respectively with the speed of 0.4mL/min, the duration is 3h.Microballoon lyophilized dry 18h, with product after scanning electron microscopic observation freeze-drying, result as shown in figure 13.By filling post in embodiment 2 for being separated with the mixed solution of glutathione, as Figure 14 containing bovine serum albumin.
Embodiment 6
Be from the different of embodiment 1 microballoon having prepared 12%, once sieve the microballoon within the scope of 50 order-100 orders, add epoxychloropropane and 50wt%NaOH respectively with the speed of 0.1mL/min, the duration is 3h.The dry 18h of freeze-drying, with product after scanning electron microscopic observation freeze-drying, result as shown in figure 15.
Embodiment 7
The mode identical with embodiment 1 is adopted to prepare complex microsphere, as different from Example 1: adopt glucan 100,000.Be separated saliferous bovine serum albumen solution with it, result as shown in figure 16.
Embodiment 8
Adopt the mode identical with embodiment 1 to prepare complex microsphere, as different from Example 1: first three hour of crosslinking agent uses epoxychloropropane, within latter three hours, use BDO two bisglycidyl ether.Be separated saliferous bovine serum albumen solution with it, result as shown in figure 17.
The foregoing is only preferred embodiments of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the preparation method of agar/glucan composite gel microsphere, comprise: steps A, in reaction vessel, add agar gel microspheres and l dextran solution, in described agar gel microspheres, the content of agar is 2 ~ 20wt%, and the concentration of dextran solution is 0.05 ~ 1.5g/ml; Step B, at 25 ~ 55 DEG C, rotating speed is 60 ~ 200rpm, adds anhydrous sodium sulfate, after dissolving, then adds NaBH
4; Add sodium sulphate and NaBH
4containing 0.1 ~ 0.25g/mlNa in rear system
2sO
4with 0.008 ~ 0.02g/mlNaBH
4; Step C, in the basic conditions, adds double-functional group crosslinking agent; Step D, reaction temperature is 40 ~ 120 degrees Celsius, and duration of the reaction is 15 ~ 25h.
2. the preparation method of a kind of agar/glucan composite gel microsphere according to claim 1, is characterized in that: in the agar gel microspheres described in steps A, the content of agar is 2 ~ 12wt%, and particle diameter is at 30 ~ 500 microns.
3. the preparation method of a kind of agar/glucan composite gel microsphere according to claim 1, is characterized in that: the molecular weight of glucan is 2 ~ 200,000.
4. the preparation method of a kind of agar/glucan composite gel microsphere according to claim 1, is characterized in that: in step C, and dripping concentration is the NaOH solution of 20 ~ 70wt%, and its addition is 0.1 ~ 5 times of agar gel microspheres volume.
5. the preparation method of a kind of agar/glucan composite gel microsphere according to claim 1, is characterized in that: the addition of crosslinking agent is 0.2 ~ 5 times of agar gel microspheres volume.
6. the preparation method of a kind of agar/glucan composite gel microsphere according to claim 1, is characterized in that: crosslinking agent is epoxychloropropane, BDO two bisglycidyl ether or pentaerythrite glycidol ether.
7. a kind of agar/application of glucan composite gel microsphere in abstraction and purification that as described in any one in claim 1 ~ 6 prepared by method.
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| US6395884B1 (en) * | 1996-09-13 | 2002-05-28 | Transkaryotic Therapies, Inc. | Therapy for α-galactosidase a deficiency |
| CN102389755A (en) * | 2011-08-02 | 2012-03-28 | 北京化工大学 | Preparation method of agar gel microspheres |
| CN103374143A (en) * | 2012-04-28 | 2013-10-30 | 中国科学院过程工程研究所 | Super macroporous polymer microspheres and preparation method thereof |
| CN103769057A (en) * | 2012-10-25 | 2014-05-07 | 中国科学院过程工程研究所 | High-strength polysaccharide aerogel microsphere, and preparation method and application thereof |
| CN103831066A (en) * | 2012-11-27 | 2014-06-04 | 山东鼎欣生物科技有限公司 | Preparation technology of CM agar-based chromatographical microspheres |
-
2014
- 2014-06-21 CN CN201410281508.3A patent/CN104043379B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS62253603A (en) * | 1986-04-08 | 1987-11-05 | Bitamin Kenkyusho:Kk | Production of spherical resin |
| CN1082068A (en) * | 1992-08-10 | 1994-02-16 | 中国科学院化工冶金研究所 | Microballon of double water-phase emulsion process preparation and preparation method thereof |
| US6395884B1 (en) * | 1996-09-13 | 2002-05-28 | Transkaryotic Therapies, Inc. | Therapy for α-galactosidase a deficiency |
| CN102389755A (en) * | 2011-08-02 | 2012-03-28 | 北京化工大学 | Preparation method of agar gel microspheres |
| CN103374143A (en) * | 2012-04-28 | 2013-10-30 | 中国科学院过程工程研究所 | Super macroporous polymer microspheres and preparation method thereof |
| CN103769057A (en) * | 2012-10-25 | 2014-05-07 | 中国科学院过程工程研究所 | High-strength polysaccharide aerogel microsphere, and preparation method and application thereof |
| CN103831066A (en) * | 2012-11-27 | 2014-06-04 | 山东鼎欣生物科技有限公司 | Preparation technology of CM agar-based chromatographical microspheres |
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