CN104031177A - Method for degrading chitosan - Google Patents
Method for degrading chitosan Download PDFInfo
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- CN104031177A CN104031177A CN201410304918.5A CN201410304918A CN104031177A CN 104031177 A CN104031177 A CN 104031177A CN 201410304918 A CN201410304918 A CN 201410304918A CN 104031177 A CN104031177 A CN 104031177A
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Abstract
The invention discloses a method for degrading chitosan. The method comprises the following steps: preparing a chitosan solution by taking diluted hydrochloric acid as a solvent, adding a hydrogen peroxide solution, keeping the temperature, storing, performing eddy cavitation degradation, adjusting the pH value of the solution, performing centrifugal separation, and freeze-drying. The method is simple in process, the degradation time of chitosan is shortened, and the degradation effect is relatively good.
Description
Technical field
The present invention relates to the degradation technique field of chitosan, especially a kind of oxidation degradation method and convolution cavitation method combine the method for degrade chitosan.
Background technology
Chitosan claims again chitosan, is that the chitin extensively being existed by nature obtains through deacetylation, has the performance of a lot of excellences, is widely used in fields such as medicine, food, environmental protection, agricultural, makeup.The molecular weight of chitosan is up to millions of, water insoluble equal solvent, badly influence the application of chitosan, and chitosan has the characteristics such as water-soluble enhancing, moisture retention is good, antibacterial ability is strong after degraded, thereby energy broader applications are in the mankind's productive life, therefore, how effectively degrade chitosan becomes the focus of numerous scholar's research.At present, the method for degrade chitosan mainly contains chemical method, Physical, and enzymolysis process, but still there is the problem that cost is high, degradation effect is undesirable of degrading in these methods.
Summary of the invention
The object of this invention is to provide a kind of degradation method of chitosan, this method can solve the problem that degraded cost is high, degradation effect is undesirable.
In order to address the above problem, the technical solution used in the present invention is: the degradation method of this chitosan, comprises the following steps:
A, taking dilute hydrochloric acid solution as solvent, preparation chitosan solution; Wherein, the concentration of described dilute hydrochloric acid is 0.2 mol/L～05 mol/L, and the amount of the described chitosan adding in every 1 liter of described dilute hydrochloric acid is 1 gram～5 grams;
B, in steps A gained solution, add hydrogen peroxide solution, insulation storage 30 minutes; Wherein, every 1 gram of chitosan adds 2 milliliters～3 milliliters described hydrogen peroxide;
C, step B gained solution is injected to convolution cavitation device degrade, degradation time is 3 hours～4 hours;
PH value to 6～8 of solution after D, adjusting degraded, centrifugation, lyophilize.
In technique scheme, technical scheme can also be more specifically: described in steps A, the concentration of dilute hydrochloric acid is 0.15 mol/L～0.2 mol/L;
Convolution cavitation pressure described in step B is 0.1 MPa～0.3 MPa.
Owing to having adopted technique scheme, the present invention compared with prior art has following beneficial effect:
1, present method combines hydrogen peroxide oxidation degraded and convolution cavitation degraded, and degradation rate is fast, has shortened the reaction times, and effect is better.
2, present method technique is simple, and products therefrom is water-soluble good.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1:
Get the dilute hydrochloric acid of 2 liter of 0.15 mol/L, then add wherein 2 grams of chitosans, then add 4 milliliters of hydrogen peroxide solutions, be incubated 30 minutes, gained solution is injected to convolution cavitation device degraded 3 hours, and pressure-controlling when degraded is in 0.1 MPa, with pH to 6 after sodium hydroxide solution adjusting degraded, centrifugation, lyophilize.
Embodiment 2:
Get the dilute hydrochloric acid of 2 liter of 0.2 mol/L, then add wherein 6 grams of chitosans, then add 15 milliliters of hydrogen peroxide solutions, be incubated 30 minutes, gained solution is injected to convolution cavitation device degraded 3.5 hours, and pressure-controlling when degraded is in 0.2 MPa, with pH to 7 after sodium hydroxide solution adjusting degraded, centrifugation, lyophilize.
Embodiment 3:
Get the dilute hydrochloric acid of 2 liter of 0.2 mol/L, then add wherein 10 grams of chitosans, then add 30 milliliters of hydrogen peroxide solutions, be incubated 30 minutes, gained solution is injected to convolution cavitation device degraded 4 hours, and pressure-controlling when degraded is in 0.3 MPa, with pH to 8 after sodium hydroxide solution adjusting degraded, centrifugation, lyophilize.
Claims (3)
1. a degradation method for chitosan, is characterized in that comprising the following steps:
A, taking dilute hydrochloric acid solution as solvent, preparation chitosan solution; Wherein, the concentration of described dilute hydrochloric acid is 0.2 mol/L～05 mol/L, and the amount of the described chitosan adding in every 1 liter of described dilute hydrochloric acid is 1 gram～5 grams;
B, in steps A gained solution, add hydrogen peroxide solution, insulation storage 30 minutes; Wherein, every 1 gram of chitosan adds 2 milliliters～3 milliliters described hydrogen peroxide;
C, step B gained solution is injected to convolution cavitation device degrade, degradation time is 3 hours～4 hours;
PH value to 6～8 of solution after D, adjusting degraded, centrifugation, lyophilize.
2. the degradation method of chitosan according to claim 1, is characterized in that: described in steps A, the concentration of dilute hydrochloric acid is 0.15 mol/L～0.2 mol/L.
3. the degradation method of chitosan according to claim 1, is characterized in that: described in step B, convolution cavitation pressure is 0.1 MPa～0.3 MPa.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410304918.5A CN104031177A (en) | 2014-06-30 | 2014-06-30 | Method for degrading chitosan |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410304918.5A CN104031177A (en) | 2014-06-30 | 2014-06-30 | Method for degrading chitosan |
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| CN104031177A true CN104031177A (en) | 2014-09-10 |
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| CN201410304918.5A Pending CN104031177A (en) | 2014-06-30 | 2014-06-30 | Method for degrading chitosan |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016799A (en) * | 2021-02-24 | 2021-06-25 | 杭州西子卫生消毒药械有限公司 | Low-temperature-resistant disinfectant and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2215749C2 (en) * | 2001-06-14 | 2003-11-10 | Комаров Борис Александрович | Method for preparing chitosan soluble forms |
| CN101864001A (en) * | 2010-05-20 | 2010-10-20 | 广西工学院 | Preparation method of oligochitosan |
| CN102321194A (en) * | 2011-08-12 | 2012-01-18 | 厦门蓝湾科技有限公司 | Method for preparing water-soluble chitosan with low molecular weight |
| CN103304686A (en) * | 2013-06-28 | 2013-09-18 | 江苏大学 | Method for preparing oligomeric narrow-molecular weight distribution chitosan oligosaccharides through ultrasonic-microwave synergetic oxidation |
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2014
- 2014-06-30 CN CN201410304918.5A patent/CN104031177A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2215749C2 (en) * | 2001-06-14 | 2003-11-10 | Комаров Борис Александрович | Method for preparing chitosan soluble forms |
| CN101864001A (en) * | 2010-05-20 | 2010-10-20 | 广西工学院 | Preparation method of oligochitosan |
| CN102321194A (en) * | 2011-08-12 | 2012-01-18 | 厦门蓝湾科技有限公司 | Method for preparing water-soluble chitosan with low molecular weight |
| CN103304686A (en) * | 2013-06-28 | 2013-09-18 | 江苏大学 | Method for preparing oligomeric narrow-molecular weight distribution chitosan oligosaccharides through ultrasonic-microwave synergetic oxidation |
Non-Patent Citations (1)
| Title |
|---|
| 陈国强等: "《纺织品整理加工用化学品》", 30 April 2009 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113016799A (en) * | 2021-02-24 | 2021-06-25 | 杭州西子卫生消毒药械有限公司 | Low-temperature-resistant disinfectant and preparation method thereof |
| CN113016799B (en) * | 2021-02-24 | 2021-10-26 | 杭州西子卫生消毒药械有限公司 | Low-temperature-resistant disinfectant and preparation method thereof |
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Application publication date: 20140910 |