CN104020195B - Two identification dopamine trace electrochemical sensor and its preparation method and application - Google Patents
Two identification dopamine trace electrochemical sensor and its preparation method and application Download PDFInfo
- Publication number
- CN104020195B CN104020195B CN201410228957.1A CN201410228957A CN104020195B CN 104020195 B CN104020195 B CN 104020195B CN 201410228957 A CN201410228957 A CN 201410228957A CN 104020195 B CN104020195 B CN 104020195B
- Authority
- CN
- China
- Prior art keywords
- dopamine
- preparation
- electrochemical sensor
- polymer film
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Abstract
The invention discloses a kind of two identification dopamine trace electrochemical sensor and its preparation method and application, the method comprises following operation: 3-aminoboronic acid, 2,5-dimethoxy furans and acetic acid are carried out the operation that contact reaction prepares function monomer by (1); (2) under the existence of buffer solution, function monomer, dopamine are carried out being mixed to form polymer fluid, and glass-carbon electrode is placed in described polymer fluid and carries out according to cyclic voltammetry scanning the operation preparing polymer film modified electrode; (3) described polymer film modified electrode is placed in acid solution and carries out the two operation identifying dopamine trace electrochemical sensor of galvanochemistry wash-out preparation.Electrochemical sensor provided by the invention has excellent specific recognition capability and antijamming capability for the detection of dopamine.
Description
Technical field
The present invention relates to electrochemical sensor and molecular imprinting field, particularly, relate to a kind of two identification dopamine trace electrochemical sensor and its preparation method and application.
Background technology
In recent years, rapidly, particularly molecularly imprinted polymer (MIPs) (referring to the polymkeric substance that target molecule (also known as template molecule or microsphere) is had to selectivity selection and identifies) has higher using value in molecular recognition field in molecular imprinting development.This technology is usually in the hole of the molecular dimension of the internal build of polymkeric substance and target molecule, spatial structure and functional group's complementation, thus the identification realized target molecule and the object be separated.The enzyme that progressively instead of in bio-sensing due to the ability of the Selective recognition molecule of MIPs brilliance and antibody, and it also possesses, and preparation is simple, cost is low, steady performance, is widely used in the fields such as bionical sensing, chromatographic resolution, enzyme simulation, Pharmaceutical Analysis and chiral resolution.Imprinting factor (IF) is an important indicator of the binding ability weighing imprinted polymer and template molecule, and specifically finger mark polymkeric substance and non-imprinted polymer are to the ratio of template molecule binding ability.
Dopamine (DA) is a kind of important neurotransmitter, plays a significant role in nervous centralis, cardiovascular, kidney, Hormone system.In prior art, the detection of dopamine can utilize the molecule of borate compound and o-dihydroxy structure covalent bond can occur in the basic conditions, generate five-membered ring boron ester complexes and the covalent bond principle that can rupture fast again in acid condition formed detects, but this detection method is subject to the interference of other o-dihydroxy structural materials (as adrenaline EP, norepinephrine NE, ascorbic acid AA and uric acid UA etc.).Also the molecular imprinting electrochemical sensor prepared based on molecular imprinting can be utilized can to carry out selective enumeration method to dopamine, but this detection method exist the coarse defect of the specific recognition of target molecule.Main cause is function monomer in electrochemical sensor and is Non-covalent binding between template molecule, imprinted sites (hole) out of true, polymer film surface monomer functional group is exposed more, thus the interference of non-specific adsorption is comparatively obvious, and imprinting factor is not high.
Summary of the invention
The object of this invention is to provide a kind of two identification dopamine trace electrochemical sensor and its preparation method and application, this electrochemical sensor has higher specific recognition capability and excellent antijamming capability to DA, and the simple and cost of this preparation method makes it have a wide range of applications in the detection of DA simultaneously.
To achieve these goals, the invention provides a kind of two preparation method identifying dopamine trace electrochemical sensor, the method comprises following operation:
(1) 3-aminoboronic acid, 2,5-dimethoxy furans and acetic acid are carried out the operation of contact reaction preparation such as formula the function monomer of structure (I) Suo Shi;
(2) under the existence of buffer solution, function monomer, dopamine are carried out being mixed to form polymer fluid, and glass-carbon electrode is placed in described polymer fluid and adopts cyclic voltammetry to carry out scanning the operation preparing polymer film modified electrode;
(3) described polymer film modified electrode is placed in acid solution and carries out the two operation identifying dopamine trace electrochemical sensor of galvanochemistry wash-out preparation;
Present invention also offers a kind of two identification dopamine trace electrochemical sensor, described electrochemical sensor is prepared by above-mentioned method.
Present invention also offers two identification dopamine trace electrochemical sensor prepared by above-mentioned method and detect the application in dopamine.
By technique scheme, the present invention is by the function monomer Py-PBA of preparation such as formula structure (I) Suo Shi, and this function monomer has boric acid base group and makes it with template molecule DA, covalent bond can occur.As shown in Figure 1, under the existence of phosphate buffer solution PBS, function monomer, DA are carried out being mixed to form polymer fluid, principal ingredient wherein in mixed liquor is the compound such as formula structure (II) Suo Shi, under electrochemical effect, there is electroinitiated polymerization on glass-carbon electrode GCE surface in the compound of structure shown in formula (II), forms the polypyrrole film of one deck densification thus obtain polymer film modified electrode in glassy carbon electrode surface.As shown in Figure 2, polymer film modified electrode wash-out under electrochemical effect is removed template molecule, thus forms three-dimensional imprinted cavity on the surface of polymer film modified electrode, final formation is two identifies dopamine trace electrochemical sensor.This electrochemical sensor is because have boric acid base group and three-dimensional imprinted cavity thus realize the dual identification to dopamine simultaneously, basic reason is: not only boric acid base group only with the molecule generation covalent bond of o-dihydroxy structure, first can eliminate the interference of non-neighboring dihydroxy compounds; And three-dimensional imprinted cavity is only complementary with template molecule three-dimensional structure, thus reduce the interference of other o-dihydroxy structural material.The low electrochemical sensor prepared that makes of simple, the cost of this preparation method's step can be widely used in the detection of DA simultaneously
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for instructions, is used from explanation the present invention, but is not construed as limiting the invention with embodiment one below.In the accompanying drawings:
Fig. 1 is two schematic diagram identifying dopamine trace electrochemical sensor produced according to the present invention;
Fig. 2 is the schematic diagram of the DA molecule according to eluted polymer film modified electrode provided by the invention;
Fig. 3 be in embodiment 2 electrochemical sensor to the Electrochemical results figure of DA;
Fig. 4 be in embodiment 3 electrochemical sensor to the Electrochemical results figure of DA;
Fig. 5 be in embodiment 4 electrochemical sensor to the Electrochemical results figure of DA;
Fig. 6 be in embodiment 5 electrochemical sensor to the Electrochemical results figure of DA;
Fig. 7 be in embodiment 6 electrochemical sensor to the Electrochemical results figure of DA;
Fig. 8 is two result statistical graph identifying the interference--free experiments of dopamine trace electrochemical sensor in test case 1;
Fig. 9 is the result statistical graph that the recognition capability to DA of two identification dopamine trace electrochemical sensor in test case 2 is tested.
Embodiment
Below the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
The invention provides a kind of two preparation method identifying dopamine trace electrochemical sensor, the method comprises following operation:
(1) 3-aminoboronic acid, 2,5-dimethoxy furans and acetic acid are carried out the operation of contact reaction preparation such as formula the function monomer of structure (I) Suo Shi;
(2) under the existence of buffer solution, function monomer, dopamine are carried out being mixed to form polymer fluid, and glass-carbon electrode is placed in described polymer fluid and adopts cyclic voltammetry to carry out scanning the operation preparing polymer film modified electrode;
(3) described polymer film modified electrode is placed in acid solution and carries out the two operation identifying dopamine trace electrochemical sensor of galvanochemistry wash-out preparation;
Prepare in the operation of function monomer of the present invention, 3-aminoboronic acid, 2, the consumption of 5-dimethoxy furans and acetic acid is selected in wide scope, in order to improve catalytic yield, preferably, relative to the 3-aminoboronic acid of 1mmol, described 2, the consumption of 5-dimethoxy furans is 1.5 ~ 2.5mmol, and the consumption of described acetic acid is 0.4 ~ 0.6mmol.
Prepare in the operation of function monomer of the present invention, be not particularly limited described catalytic temperature of reaction, in order to accelerate catalytic reaction rate, preferably, described catalytic temperature is 80 ~ 100 DEG C.
Prepare in the operation of function monomer of the present invention, in order to improve catalytic yield, the described catalytic time is 6 ~ 10h.
Prepare in the operation of function monomer of the present invention, 3-aminoboronic acid, 2,5-dimethoxy furans and acetic acid can three mix after directly carry out contact reaction, also contact reaction can be carried out in organic solvent, in order to improve catalytic yield, preferably, described contact reaction is carried out in organic solvent.In order to improve catalytic yield further, more preferably, relative to the 3-aminoboronic acid of 1mmol, the consumption of described organic solvent is 10 ~ 30mL.For the concrete reagent of organic reagent, there is no particular limitation, as long as can dissolve 3-aminoboronic acid, 2,5-dimethoxy furans and acetic acid, and not with 3-aminoboronic acid, 2,5-dimethoxy furans and acetic acidreaction, taking cost into account, preferred described organic solvent is selected from acetonitrile.
Prepare in the operation of polymer film modified electrode of the present invention, the consumption of function monomer and dopamine can be selected in wide scope, in order to improve the productive rate such as formula the compound of structure (II) Suo Shi in reactant liquor, preferably, relative to 1 μm of ol dopamine, the consumption of described function monomer is 1.0 ~ 1.6 μm of ol.In order to improve the yield of the compound such as formula structure (II) Suo Shi further, function monomer and dopamine make function monomer solution and dopamine solution mixes again, the solvent of function monomer solution is water: ethanol (V/V=1), and the solvent of dopamine solution is water: ethanol (V/V=1).In order to further improve the productive rate of the compound such as formula structure (II) Suo Shi, the concentration of dopamine solution is 1mmol/L, and the concentration of function monomer solution is 1.2mmol/L, and the volume ratio of the two is 1:1 ~ 1.6.
Prepare in the operation of polymer film modified electrode of the present invention, to the concrete kind of buffer solution, there is no particular limitation, as long as the generation of the compound of structure shown in formula (II) can not be affected, in order to improve the productive rate of the compound such as formula structure (II) Suo Shi, preferably, relative to 1 μm of ol dopamine, the pH of described buffer solution is 5 ~ 9 and volume is 0.6 ~ 2mL; Due to when the pH of buffer solution is below 7, it is noncovalent interaction between the molecule of borate compound and o-dihydroxy structure, this mode of action is unstable, in order to make the molecule generation covalent bond of borate compound and o-dihydroxy structure, more preferably, relative to 1 μm of ol dopamine, the pH of described buffer solution is 7 ~ 8.5 and volume is 1 ~ 1.6mL.Further preferably, described buffer solution is phosphate buffer solution.
Prepare in the operation of polymer film modified electrode of the present invention, in order to the adhesion of the polypyrrole film and GCE that improve formation, preferably, in the operation preparing polymer film modified electrode, current potential-0.2 ~ 1.2V of described scanning, the number of turns of described scanning is 10 ~ 30 circles, and the speed of described scanning is 25 ~ 100mV/s.
In the operation of preparation of the present invention two identification dopamine trace electrochemical sensor, to acid solution, there is no particular limitation, can be H
2sO
4, HCl, CH
3cOOH and H
3pO
4in one or more, in order to accelerate elution rate, preferably, the pH of described acid solution is 0 ~ 3.In order to accelerate elution rate further, more preferably, the current potential 0 ~ 1.5V of described wash-out, the number of turns of described wash-out is 10 ~ 30 circles,
In the present invention, in order to improve the stability of the polymer film modified electrode of preparation, preferably, before preparing the operation of polymer film modified electrode, described method also comprises carries out pretreated operation by described glass-carbon electrode: carried out by described glass-carbon electrode polishing on chamois leather and the described glass-carbon electrode after polishing is placed in K
3[Fe (CN)
6]/K
4[Fe (CN)
6] carry out electrochemical treatment until the current potential value difference △ Ep < 75mV of oxidation peak and reduction peak according to cyclic voltammetry in solution, then carry out ultrasonic cleaning with second alcohol and water respectively.In order to improve the quality of the polymer film modified electrode of preparation further, more preferably, described K
3[Fe (CN)
6]/K
4[Fe (CN)
6] concentration of solution is 0.1mmol/L ~ 10mmol/L.In addition, described K
3[Fe (CN)
6]/K
4[Fe (CN)
6] solution can be prepared, in accordance with the following methods namely by getting the K of 0.1mmol
3[Fe (CN)
6], the K of 0.1mmol
4[Fe (CN)
6] and 0.1molKCl mixing to be dissolved in deionized water obtained.
In the present invention, in order to improve the quality of the polymer film modified electrode of preparation, preferably, before described glass-carbon electrode is placed in described polymer fluid, described method also comprises pass into nitrogen in described polymer fluid.More preferably, the time passing into nitrogen described in is 5 ~ 30mim.
Present invention also offers a kind of two identification dopamine trace electrochemical sensor, described electrochemical sensor is prepared by above-mentioned method.
Present invention also offers two identification dopamine trace electrochemical sensor prepared by above-mentioned method and detect the application in dopamine.
Below will be described the present invention by embodiment and test case.In following examples and test case, the test of cyclic voltammetric parameter is undertaken by electrochemical workstation CHI830C, the test of differential pulse voltametry parameter is undertaken by electrochemical workstation CHI830C, galvanochemistry wash-out is undertaken by electrochemical workstation CHI830C, and the test of nucleus magnetic hydrogen spectrum parameter is undertaken by German BrukerAV300 nuclear magnetic resonance analyser.
3-aminoboronic acid is the product of Shanghai Bai Ka chemical technology company limited, 2,5-dimethoxy furans is the product of Aladdin company, dopamine (DA) is the product of Sigma-Aldrich company, ascorbic acid (AA) is the product of company of Chemical Reagent Co., Ltd., Sinopharm Group, uric acid (UA) is the product of Sigma-Aldrich company, and adrenaline (EP) is the product of Sigma-Aldrich company, K
3[Fe (CN)
6] be the product of Solution on Chemical Reagents in Shanghai company of Chinese Medicine group, K
4[Fe (CN)
6] be the product of Chinese Shanghai experiment reagent company limited.
Embodiment 1
1) preparation of function monomer
The 3-aminobenzene boric acid getting 100mg (0.646mmol) is dissolved in 10mL acetonitrile, the acetic acid of 0.167mL (1.292mmol) 2,5-dimethoxy-tetrahydrofuran and 0.018mL (0.323mmol) is slowly added under the condition stirred.Reflux at above-mentioned mixed solution is heated to 80 DEG C 6h, obtains the thick liquid of tawny.After extraction, column chromatography are purified, obtain dark pure solution.In immigration Rotary Evaporators, heating evaporation at 80 DEG C, obtains pale yellow powder solid product, i.e. Py-PBA.
The characterization data of Py-PBA is:
1hNMR (CD
3oD, 300MHz): δ (ppm): 6.262 (bs, 2H, Pyr-H), 7.154 (s, 2H, BOH), 7.417-7.465 (m, 1H, Ar-H), 7.634 (d, 1H, Ar-H), 7.829 (bs, 1H, Ar-H).
2) preparation of polymer film modified electrode
At K after glass-carbon electrode is polished on chamois leather
3[Fe (CN)
6]/K
4[Fe (CN)
6] adopt in solution (concentration is 0.1mmol) cyclic voltammetry (CV) to process, until use the ultrasonic cleaning of second alcohol and water again after the spike potential value difference △ Ep < 75mV of oxidation peak and reduction peak and dry.The stoste 100 μ L getting the DA of Py-PBA stoste 120 μ L, 0.05mol/L of 0.05mol/L is added to the phosphate buffered solution (PBS of the 0.05mol/L of 5mL, pH=8.0) in, the concentration of rear pattern plate molecule DA is 1mmol/L, the concentration of function monomer is 1.2mmol/L, mixing concussion forms homogeneous polymer fluid, letting nitrogen in and deoxidizing 5min in polymer fluid.Immersed by glass-carbon electrode after above-mentioned process in polymer fluid, adopt the process of CV method, in-0.2V ~ 1.2V potential range, scan round 20 is enclosed, and sweeps fast 50mV/s, just obtained polymer film modified electrode.
3) preparation of two identification dopamine trace electrochemical sensor
By the H of polymer film modified electrode at 0.5mol/L
2sO
4electrochemically eluted template molecule DA in solution, wash-out current potential is at 0V ~ 1.5V, and wash-out 25 encloses rear obtained electrochemical sensor A1.
Embodiment 2
Carry out according to the method for embodiment 1, the value not being both the buffer solution pH in the morning in the operation preparing polymer film modified electrode is respectively 5,6,7,8,9 obtained electrochemical sensors, and be working electrode by electrochemical sensor, in the PBS solution of the 0.05mol/L of pH=7.0, detect 1 × 10 by DPV method
-4the current signal of mol/L dopamine, scanning current potential is-0.2 ~ 0.6V.As shown in Figure 3, obtain electrochemical sensor when pH is 8 has the strongest electrochemical sensing to DA to testing result.
Embodiment 3
Carry out according to the method for embodiment 1, the volume not being both the function monomer solution of 0.05mol/L in the operation preparing polymer film modified electrode is respectively 100 μ L, 120 μ L, 140 μ L and 160 μ L, namely the mol ratio of function monomer and template molecule DA is respectively 1.0,1.2,1.4,1.6 obtained electrochemical sensors, and be working electrode by electrochemical sensor, in the PBS solution of the 0.05mol/L of pH=7.0, detect 1 × 10 by DPV method
-4the current signal of mol/L dopamine, scanning current potential is-0.2 ~ 0.6V.As shown in Figure 4, obtain electrochemical sensor when the mol ratio of function monomer and template molecule DA is 1.2 has the strongest electrochemical sensing to DA to testing result.
Embodiment 4
Carry out according to the method for embodiment 1, the scan round number of turns be not both in the operation preparing polymer film modified electrode is respectively the obtained electrochemical sensor of 10,15,20,25,30 circles, and be working electrode by electrochemical sensor, in the PBS solution of the 0.05mol/L of pH=7.0, detect 1 × 10 by DPV method
-4the current signal of mol/L dopamine, scanning current potential is-0.2 ~ 0.6V.As shown in Figure 5, obtain electrochemical sensor when the scan round number of turns is 20 circle has the strongest electrochemical sensing to DA to testing result.
Embodiment 5
Carry out according to the method for embodiment 1, the sweep speed be not both in the operation preparing polymer film modified electrode is respectively 25,50,75, the obtained electrochemical sensor of 100mV/s, and be working electrode by electrochemical sensor, in the PBS solution of the 0.05mol/L of pH=7.0, detect 1 × 10 by DPV method
-4the current signal of mol/L dopamine, scanning current potential is-0.2 ~ 0.6V.As shown in Figure 6, obtain electrochemical sensor when sweep speed is 50mV/s has the strongest electrochemical sensing to DA to testing result.
Embodiment 6
Carry out according to the method for embodiment 1, the wash-out number of turns be not both in the preparation section of two identification dopamine trace electrochemical sensor is respectively the obtained electrochemical sensor of 10,15,20,25,30 circles, and be working electrode by electrochemical sensor, in the PBS solution of the 0.05mol/L of pH=7.0, detected the current signal of 1 × 10-4mol/L dopamine by DPV method, scanning current potential is-0.2 ~ 0.6V.As shown in Figure 7, obtain electrochemical sensor when the wash-out number of turns is respectively 25 has the strongest electrochemical sensing to DA to testing result.
Comparative example 1
Carry out according to the method for embodiment 1, be not both not containing DA solution in the operation preparing polymer film modified electrode, obtained electrochemical sensor B1.
Test case 1
In liquid at the bottom of the PBS of the 0.05mol/L of pH=7.0, add DA respectively, with chaff interference AA, UA and EP of DA equivalent, chaff interference UA, EP and AA of 10 times of DA amounts, the chaff interference AA of 100 times of DA amounts, wherein the concentration of DA is 1 × 10
-5mol/L.Using sensors A 1 as working electrode, detected the current signal of DA oxidation peak by differential pulse voltametry (DPV), scanning current potential is-0.2V ~ 0.6V.As shown in Figure 8, under 10 times of concentration interfering materials coexist, sensors A 1 for the current-responsive of DA without remarkable change.In human body, the concentration of AA is far above the concentration of DA, therefore, the AA that we add 100 times in DA solution detects, result shows, the AA of high concentration still without obviously interference to the detection of DA, therefore absolutely proves that this sensor has good Selective recognition ability to template molecule DA, namely has excellent antijamming capability to the detection of DA.
Test case 2
In the PBS solution of the 0.05mol/L of pH=7.0, be working electrode with sensors A 1 and sensor B1 respectively, detect 2 × 10 by DPV method
-5the current signal of mol/L dopamine, scanning current potential is-0.2 ~ 0.6V.As shown in Figure 9, sensors A 1 only has obvious current-responsive to template molecule DA, and to other interfering materials all without significantly response, illustrate that A1 has good recognition capability for DA, simultaneously compared with the current-responsive of B1, the low current signal produced due to B1 is produced by non-specific adsorption, illustrates that sensors A 1 effectively can suppress the interference of non-specific adsorption.
More than describe the preferred embodiment of the present invention in detail; but the present invention is not limited to the detail in above-mentioned embodiment, within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out combination in any between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (7)
1. the two preparation method identifying dopamine trace electrochemical sensor, it is characterized in that, the method comprises following operation:
(1) 3-aminobenzene boric acid, 2,5-dimethoxy-tetrahydrofurans and acetic acid are carried out in acetonitrile the operation of contact reaction preparation such as formula the function monomer of structure (I) Suo Shi;
(2) under the existence of phosphate buffer solution, function monomer, dopamine are carried out being mixed to form polymer fluid, then in described polymer fluid, pass into nitrogen, then glass-carbon electrode is placed in described polymer fluid and adopts cyclic voltammetry to carry out scanning the operation preparing polymer film modified electrode;
(3) described polymer film modified electrode is placed in acid solution and carries out the two operation identifying dopamine trace electrochemical sensor of galvanochemistry wash-out preparation;
Wherein, in the operation preparing function monomer, relative to the 3-aminobenzene boric acid of 1mmol, described 2, the consumption of 5-dimethoxy-tetrahydrofuran is 1.5 ~ 2.5mmol, and the consumption of described acetic acid is 0.4 ~ 0.6mmol, and the consumption of described acetonitrile is 10 ~ 50mL, described catalytic temperature is 80 ~ 100 DEG C, and the described catalytic time is 6 ~ 10h; In the operation preparing polymer film modified electrode, relative to 1 μm of ol dopamine, the consumption of described function monomer is 1.0 ~ 1.6 μm of ol, and the pH of described phosphate buffer solution is 5 ~ 9 and volume is 0.6 ~ 2mL; In the operation preparing polymer film modified electrode, current potential-0.2 ~ 1.2V of described scanning, the number of turns of described scanning is 10 ~ 30 circles, and the speed of described scanning is 25 ~ 100mV/s; In the operation of preparation two identification dopamine trace electrochemical sensor, the pH of described acid solution is 0 ~ 3, the current potential 0 ~ 1.5V of described wash-out, and the number of turns of described wash-out is 10 ~ 30 circles.
2. preparation method according to claim 1, wherein, relative to dopamine described in 1 μm of ol, the pH of described phosphate buffer solution solution is 7 ~ 8.5 and volume is 1 ~ 1.6mL.
3. preparation method according to claim 1 and 2, wherein, before preparing the operation of polymer film modified electrode, the method also comprises carries out pretreated operation by described glass-carbon electrode: carried out by described glass-carbon electrode polishing on chamois leather and the described glass-carbon electrode after polishing is placed in K
3[Fe (CN)
6]/K
4[Fe (CN)
6] adopt cyclic voltammetry to carry out electrochemical treatment until the current potential value difference △ Ep < 75mV of oxidation peak and reduction peak in solution, then carry out ultrasonic cleaning with second alcohol and water respectively.
4. preparation method according to claim 3, wherein, described K
3[Fe (CN)
6]/K
4[Fe (CN)
6] concentration of solution is 0.1mmol/L ~ 10mmmol/L.
5. preparation method according to claim 1 and 2, wherein, the time passing into described nitrogen is 5 ~ 30mim.
6. a two identification dopamine trace electrochemical sensor, it is characterized in that, described electrochemical sensor is obtained by the method preparation described in any one in claim 1-5.
7. prepared by the method according to any one in claim 1-5, two identification dopamine trace electrochemical sensor is detecting the application in dopamine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410228957.1A CN104020195B (en) | 2014-05-27 | 2014-05-27 | Two identification dopamine trace electrochemical sensor and its preparation method and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410228957.1A CN104020195B (en) | 2014-05-27 | 2014-05-27 | Two identification dopamine trace electrochemical sensor and its preparation method and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104020195A CN104020195A (en) | 2014-09-03 |
| CN104020195B true CN104020195B (en) | 2016-03-09 |
Family
ID=51437069
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410228957.1A Expired - Fee Related CN104020195B (en) | 2014-05-27 | 2014-05-27 | Two identification dopamine trace electrochemical sensor and its preparation method and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104020195B (en) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105223248B (en) * | 2015-09-21 | 2018-04-06 | 安徽师范大学 | Based on phenyl boric acid imprinted polymer/carbon nano tube modified electrode and its preparation method and application |
| CN105675559B (en) * | 2016-01-14 | 2018-01-30 | 中国科学院理化技术研究所 | A kind of method by the use of carbon point as fluorescence probe detection dopamine |
| CN105776183B (en) * | 2016-05-16 | 2017-09-29 | 安徽工业大学 | A kind of preparation method and applications of ferrocenyl carbon nano tube compound material |
| CN106645324B (en) * | 2016-11-30 | 2019-04-09 | 辽宁大学 | A kind of microsphere polymer and molecular imprinting electrochemical sensor based on boric acid base group |
| CN109438620B (en) * | 2018-10-10 | 2021-04-06 | 青岛大学 | Molecularly imprinted polymer with reversible covalent binding function and preparation method and application thereof |
| CN109593113B (en) * | 2018-10-18 | 2022-05-03 | 北京大学 | Stepwise molecular imprinting method and molecularly imprinted material |
| CN109254040B (en) * | 2018-10-25 | 2020-09-25 | 济南大学 | Bimolecular sandwich dopamine-recognizing electrochemical sensor |
| CN110687183B (en) * | 2019-10-30 | 2022-06-07 | 安徽师范大学 | Method for detecting ovalbumin by double-signal-double-recognition imprinting electrochemical sensor |
| CN111289481B (en) * | 2019-12-27 | 2021-09-17 | 中国农业科学院农业质量标准与检测技术研究所 | Biogenic amine coordination imprinted material, biogenic amine fluorescence array sensor, preparation method and application thereof |
| CN111420643B (en) * | 2020-04-15 | 2022-09-13 | 西安交通大学医学院第二附属医院 | Hydrophilic bifunctional monomer anthocyanin molecular imprinting magnetic nanospheres and preparation method and application thereof |
| CN113406169B (en) * | 2021-05-14 | 2022-06-14 | 杭州电子科技大学 | Acupuncture needle imprinting electrochemical sensor for detecting dopamine and preparation process thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101056845A (en) * | 2004-09-13 | 2007-10-17 | H·隆德贝克有限公司 | Substituted aniline derivatives |
-
2014
- 2014-05-27 CN CN201410228957.1A patent/CN104020195B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101056845A (en) * | 2004-09-13 | 2007-10-17 | H·隆德贝克有限公司 | Substituted aniline derivatives |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104020195A (en) | 2014-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104020195B (en) | Two identification dopamine trace electrochemical sensor and its preparation method and application | |
| Zhong et al. | Pyrrole–phenylboronic acid: A novel monomer for dopamine recognition and detection based on imprinted electrochemical sensor | |
| CN106198701B (en) | A kind of metal-organic framework material area load molecular imprinted polymer membrane is used for the electrochemical detection method of orthene | |
| CN103604849B (en) | Electrochemical sensor capable of simultaneously detecting dopamine, ascorbic acid and uric acid | |
| Mokhtari et al. | Analytical applications of calixarenes from 2005 up-to-date | |
| Alizadeh et al. | Promethazine determination in plasma samples by using carbon paste electrode modified with molecularly imprinted polymer (MIP): Coupling of extraction, preconcentration and electrochemical determination | |
| Li et al. | A strategy for constructing sensitive and renewable molecularly imprinted electrochemical sensors for melamine detection | |
| Li et al. | Molecularly imprinted polymer-based sensors for atrazine detection by electropolymerization of o-phenylenediamine | |
| CN105223248A (en) | Based on phenyl boric acid imprinted polymer/carbon nano tube modified electrode and its preparation method and application | |
| CN102721727B (en) | Electrochemical sensor based on molecular engram and preparation method and application thereof | |
| CN102495038B (en) | Optical ion sensing film for detecting metal ions, and preparation method and application thereof | |
| CN107607638A (en) | The detection method and kit of aromatic compound | |
| CN106323893A (en) | Palladium-ion multi-channel response probe and synthesis method and application thereof | |
| CN105353007A (en) | Preparation method of coumarin molecularly-imprinted electrochemical sensor | |
| CN103232572B (en) | Molecular imprinting polymer for roxarsone detection, and preparation method thereof | |
| CN112881483B (en) | Preparation method and application of molecularly imprinted electrochemical sensor for determining vomitoxin | |
| CN104165909A (en) | Biological electrochemical detection method of fumaric acid | |
| Kim et al. | Functional polyterthiophene-appended uranyl-salophen complex: Electropolymerization and ion-selective response for monohydrogen phosphate | |
| CN103713035B (en) | The electrochemical detection method of okadaic acid in shellfish | |
| CN101419186B (en) | Self-assembling electrode with resveratrol molecular imprinting and method for making same | |
| CN104655708A (en) | P-aminobenzene sulfonic acid/imprinted poly-o-phenylenediamine modified electrode as well as preparation method and application thereof | |
| Dai et al. | Rigidified tripodal chiral ligands in the asymmetric recognition of amino compounds | |
| CN101921370A (en) | Method for preparing molecularly imprinted polymer used for detecting valnemulin | |
| CN105223260A (en) | Trace is electrochemical sensor detecting butyl p-hydroxybenzoate and preparation method thereof fast | |
| CN109254046A (en) | A kind of preparation method and application of Nitrofuran antibiotics sensor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160309 Termination date: 20180527 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |