CN104014811A - Method for manufacturing coralline nanometer cobalt by using octreotide acetate as a template - Google Patents
Method for manufacturing coralline nanometer cobalt by using octreotide acetate as a template Download PDFInfo
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Abstract
A method for manufacturing coralline nanometer cobalt by using octreotide acetate as a template mainly comprises the steps that the octreotide acetate is dissolved through pH2-3 hydrochloric acid, the octreotide acetate is regulated into an acid solution, a cobalt chloride solution is added in the regulated solution with the mole ratio of 1:10-20, the solution is then put in a water bath constant temperature oscillator, and hatching is carried out for 20-26 hours under the temperature of 13-25 DEG C at 100-200 rpm.; then, the reducing agent sodium borohydride is added in the hatched solution at a time so that the hatched solution is changed into dark black from light pink, and the black product is octreotide acetate-nanometer cobalt particles, wherein the mole ratio of the reducing agent sodium borohydride and the cobalt chloride is 1:25-35. The method for manufacturing the coralline nanometer cobalt by using the octreotide acetate as the template has the advantages that the manufacturing technology is simple, conditions are mild, the coralline nanometer cobalt is cheap and easy to obtain, the reaction is easy to control, and the productivity is high.
Description
Technical field
The invention belongs to nano metal material technical field, particularly a kind of preparation method of nano material.
Background technology
Nanometer cobalt particle has many purposes at industrial circles such as electronics industry, magnetic, carbide alloy, surface spraying, chemical catalysis as high-performance magnetism recording materials, magnetic fluid, absorbing material, activated sintering additive etc.
The preparation method of nanometer cobalt particle has thermal decomposition method, precipitation-thermal decomposition method, hydrogen reduction method, microemulsion method etc.Some technical requirement of above-mentioned preparation method is high, apparatus expensive, and some complex process, environmental pollution is larger, and the reaction time that some need to be longer, higher temperature and pressure and special device anyway, cause production cost higher.In recent years, what the method for preparing nanometer cobalt was comparatively active is liquid phase reduction, but the nano cobalt granule particle diameter of existing liquid phase reduction preparation technology gained is larger, particle diameter wider distribution, and products therefrom is easily reunited, and is difficult to extensive use.
Summary of the invention
The octreotide acetate that utilizes that the object of the present invention is to provide that a kind of preparation cost is cheap, pollution-free, technique is simple, controlled is prepared the method for coralloid nano cobalt for template, solves that liquid phase reduction can not make that particle diameter is little, a difficult problem for good dispersion and the single nano cobalt granule of composition.The present invention is mainly that employing octreotide acetate molecule is template, perfect and the strict molecular recognition function of utilizing biomolecule to have, by physico-chemical process according to designing requirement at its surperficial avtive spot located growth nano particle, architectural feature and the space confinement effect thereof of biomolecule self can accurately be controlled the size of nano particle and pattern simultaneously, thereby obtain the coralloid nano cobalt particle of expection.
Technical scheme of the present invention is as follows:
(1) get octreotide acetate, taking every milliliter of pH value as 2～3 hydrochloric acid solutions dissolve 0.4～0.8mg octreotide acetates, be mixed with pH value and be 0.4～0.8mM acidity octreotide acetate solution of 2～3;
(2) in the octreotide acetate solution that 1:10～20 ratio has been reconciled to step (1) in molar ratio, add cobalt chloride solution, preferably add the cobalt chloride solution with octreotide acetate solution phase same volume, and put it in water-bath constant temperature oscillator, in 100～200rpm., hatch 20～26h at 13～25 DEG C;
(3) in the solution of dropwise hatching to step (2), the disposable borane reducing agent sodium hydride that to add with cobalt chloride mol ratio be 1:25～35, makes it become aterrimus from pale pink, and this aterrimus product is octreotide acetate-nanometer cobalt particle.
The present invention compared with prior art tool has the following advantages:
1, to adopt octreotide acetate be template in the present invention, its molecular formula is H-D-Phe-Cys-Phe-D-Trp-Lys-Thr-Cys-Thr-OL (Cys2-Cys7), its molecular structure is simple, active group is clear, be easy to analysis and control, and pass through electrostatic interaction, object ion is easy to be adsorbed onto the surface-active site of octreotide acetate, by controlling reduction rate and reducing degree, and then the nanometer cobalt particle of generation pattern rule, so octreotide acetate has the characteristic of the good biological template of the nanometer cobalt particle of preparing homogeneous.
2, the good dispersion of prepared coralliform cobalt nanoparticle, pattern typical case, form rule, structure are controlled.
3, preparation technology is simple, mild condition, and preparation cost is cheap, course of reaction is easy to control, and is easy to obtain expected results, to equipment require low.
4, the reducing agent, reaction medium and the reactant price that adopt are low, pollution-free.
Brief description of the drawings
Fig. 1 is the TEM figure of octreotide acetate-nanometer cobalt particle of obtaining of the embodiment of the present invention 1.
Fig. 2 is the TEM figure of octreotide acetate-nanometer cobalt particle of obtaining of the embodiment of the present invention 2.
Fig. 3 is the EDS figure of octreotide acetate-nanometer cobalt particle of obtaining of the embodiment of the present invention 2.
Fig. 4 is the TEM figure of octreotide acetate-nanometer cobalt particle of obtaining of the embodiment of the present invention 3.
Detailed description of the invention
Embodiment 1
The octreotide acetate (the biochemical Shanghai of gill Co., Ltd produces) of getting 0.4mg, is dissolved in the hydrochloric acid solution of 1mLpH2, and the pH value that makes octreotide acetate solution is 2; Get above-mentioned octreotide acetate solution 200 μ L, add the cobalt chloride solution (West Asia, Shanghai reagent Co., Ltd) of 200 μ L4mM; The above-mentioned solution preparing is put into water-bath constant temperature oscillator, in 100rpm., at 13 DEG C, hatch after 26h, the disposable 50mM of adding borane reducing agent sodium hydride (production of Beijing Zhong Sheng Hua Teng Science and Technology Ltd.) 80 μ L reduce, make it become aterrimus from pale pink, obtain octreotide acetate-nanometer cobalt particle.
Application transmission electron microscope carries out morphology characterization to coralloid nano cobalt particle, and as shown in Figure 1, the diameter of coralloid nano cobalt particle is 55nm left and right, and pattern rule, is evenly distributed.
Embodiment 2
The octreotide acetate (the biochemical Shanghai of gill Co., Ltd produces) of getting 0.6mg, is dissolved in the hydrochloric acid solution of 1mLpH2.5, and the pH value that makes octreotide acetate solution is 2.5; Get above-mentioned octreotide acetate solution 200 μ L, add the cobalt chloride solution (West Asia, Shanghai reagent Co., Ltd) of 200 μ L6mM; The above-mentioned solution preparing is put into water-bath constant temperature oscillator, in 150rpm., at 20 DEG C, hatch after 24h, the disposable 40mM of adding borane reducing agent sodium hydride (production of Beijing Zhong Sheng Hua Teng Science and Technology Ltd.) 90 μ L reduce, make it become aterrimus from pale pink, obtain octreotide acetate-nanometer cobalt particle.
Application transmission electron microscope carries out morphology characterization to coralloid nano cobalt particle, and as shown in Figure 2, the diameter of coralloid nano cobalt particle is 60-70nm left and right, and pattern rule, is evenly distributed.
Application energy disperse spectroscopy characterizes coralloid nano cobalt particle, as inscribe as shown in the of 3, in power spectrum, there is Co, C, O, the peak that Cl element is corresponding, description taken in conjunction is cobalt at the metallic element on octreotide acetate surface, and O element is the element in albumen, and C element is the element in copper mesh, in addition in albumen, also can there are some C elements, illustrate that octreotide acetate is combined well with cobalt particle, and purity is very high, Cl element carrys out not cobalt chloride thoroughly of autoreduction.
Embodiment 3
The octreotide acetate (the biochemical Shanghai of gill Co., Ltd produces) of getting 0.8mg, is dissolved in the hydrochloric acid solution of 1mLpH3, and the pH value that makes octreotide acetate solution is 3; Get above-mentioned octreotide acetate solution 200 μ L, add the cobalt chloride solution (West Asia, Shanghai reagent Co., Ltd) of 200 μ L8mM; The above-mentioned solution preparing is put into water-bath constant temperature oscillator, in 200rpm., at 25 DEG C, hatch after 20h, the disposable 32mM of adding borane reducing agent sodium hydride (production of Beijing Zhong Sheng Hua Teng Science and Technology Ltd.) 100 μ L reduce, make it become aterrimus from pale pink, obtain octreotide acetate-nanometer cobalt particle.
Application transmission electron microscope carries out morphology characterization to coralloid nano cobalt particle, and as shown in Figure 4, the diameter of coralloid nano cobalt particle is 40-50nm left and right, and pattern rule, is evenly distributed.
Claims (2)
1. utilize octreotide acetate to prepare the method for coralloid nano cobalt for template, it is characterized in that:
(1) get octreotide acetate, taking every milliliter of pH value as 2～3 hydrochloric acid solutions dissolve 0.4～0.8mg octreotide acetates, be mixed with pH value and be 0.4～0.8mM acidity octreotide acetate solution of 2～3;
(2) in the solution that 1:10～20 ratio has been reconciled to step (1) in molar ratio, add cobalt chloride solution, and put it in water-bath constant temperature oscillator, in 100～200rpm., hatch 20～26h at 13～25 DEG C;
(3) the borane reducing agent sodium hydride that to add with cobalt chloride mol ratio in disposable solution of hatching to step (2) be 1:25～35, makes it become aterrimus from pale pink, can obtain the good coralloid nano cobalt particle of monodispersity.
2. the octreotide acetate that utilizes according to claim 1 is prepared the method for coralloid nano cobalt for template, it is characterized in that: preferably add the cobalt chloride solution with octreotide acetate solution phase same volume.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105312591A (en) * | 2015-11-13 | 2016-02-10 | 燕山大学 | Method for preparing annular platinum nanoparticles with lanreotide acetate serving as template |
| WO2016049138A1 (en) * | 2014-09-23 | 2016-03-31 | Attostat, Inc. | Fuel additive composition and related method |
| US9434006B2 (en) | 2014-09-23 | 2016-09-06 | Attostat, Inc. | Composition containing spherical and coral-shaped nanoparticles and method of making same |
| CN106735289A (en) * | 2016-11-30 | 2017-05-31 | 燕山大学 | A kind of method for preparing cubic silver nanoparticle box as template with octreotide acetate |
| US9839652B2 (en) | 2015-04-01 | 2017-12-12 | Attostat, Inc. | Nanoparticle compositions and methods for treating or preventing tissue infections and diseases |
| US9849512B2 (en) | 2011-07-01 | 2017-12-26 | Attostat, Inc. | Method and apparatus for production of uniformly sized nanoparticles |
| US9883670B2 (en) | 2014-09-23 | 2018-02-06 | Attostat, Inc. | Compositions and methods for treating plant diseases |
| US9919363B2 (en) | 2014-09-23 | 2018-03-20 | Attostat, Inc. | System and method for making non-spherical nanoparticles and nanoparticle compositions made thereby |
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| US10201571B2 (en) | 2016-01-25 | 2019-02-12 | Attostat, Inc. | Nanoparticle compositions and methods for treating onychomychosis |
| US10774429B2 (en) | 2015-04-13 | 2020-09-15 | Attostat, Inc. | Anti-corrosion nanoparticle compositions |
| US11018376B2 (en) | 2017-11-28 | 2021-05-25 | Attostat, Inc. | Nanoparticle compositions and methods for enhancing lead-acid batteries |
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| US11646453B2 (en) | 2017-11-28 | 2023-05-09 | Attostat, Inc. | Nanoparticle compositions and methods for enhancing lead-acid batteries |
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Cited By (19)
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|---|---|---|---|---|
| US10137503B2 (en) | 2011-07-01 | 2018-11-27 | Attostat, Inc. | Method and apparatus for production of uniformly sized nanoparticles |
| US10610934B2 (en) | 2011-07-01 | 2020-04-07 | Attostat, Inc. | Method and apparatus for production of uniformly sized nanoparticles |
| US9849512B2 (en) | 2011-07-01 | 2017-12-26 | Attostat, Inc. | Method and apparatus for production of uniformly sized nanoparticles |
| WO2016049138A1 (en) * | 2014-09-23 | 2016-03-31 | Attostat, Inc. | Fuel additive composition and related method |
| US9434006B2 (en) | 2014-09-23 | 2016-09-06 | Attostat, Inc. | Composition containing spherical and coral-shaped nanoparticles and method of making same |
| US10190253B2 (en) | 2014-09-23 | 2019-01-29 | Attostat, Inc | Nanoparticle treated fabrics, fibers, filaments, and yarns and related methods |
| US9883670B2 (en) | 2014-09-23 | 2018-02-06 | Attostat, Inc. | Compositions and methods for treating plant diseases |
| US9885001B2 (en) | 2014-09-23 | 2018-02-06 | Attostat, Inc. | Fuel additive composition and related methods |
| US9919363B2 (en) | 2014-09-23 | 2018-03-20 | Attostat, Inc. | System and method for making non-spherical nanoparticles and nanoparticle compositions made thereby |
| US9839652B2 (en) | 2015-04-01 | 2017-12-12 | Attostat, Inc. | Nanoparticle compositions and methods for treating or preventing tissue infections and diseases |
| US10953043B2 (en) | 2015-04-01 | 2021-03-23 | Attostat, Inc. | Nanoparticle compositions and methods for treating or preventing tissue infections and diseases |
| US10774429B2 (en) | 2015-04-13 | 2020-09-15 | Attostat, Inc. | Anti-corrosion nanoparticle compositions |
| US11473202B2 (en) | 2015-04-13 | 2022-10-18 | Attostat, Inc. | Anti-corrosion nanoparticle compositions |
| CN105312591A (en) * | 2015-11-13 | 2016-02-10 | 燕山大学 | Method for preparing annular platinum nanoparticles with lanreotide acetate serving as template |
| US10201571B2 (en) | 2016-01-25 | 2019-02-12 | Attostat, Inc. | Nanoparticle compositions and methods for treating onychomychosis |
| CN106735289B (en) * | 2016-11-30 | 2018-09-04 | 燕山大学 | A method of preparing cubic silver nanoparticle box by template of octreotide acetate |
| CN106735289A (en) * | 2016-11-30 | 2017-05-31 | 燕山大学 | A kind of method for preparing cubic silver nanoparticle box as template with octreotide acetate |
| US11018376B2 (en) | 2017-11-28 | 2021-05-25 | Attostat, Inc. | Nanoparticle compositions and methods for enhancing lead-acid batteries |
| US11646453B2 (en) | 2017-11-28 | 2023-05-09 | Attostat, Inc. | Nanoparticle compositions and methods for enhancing lead-acid batteries |
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