CN104007105A - Bioluminescence detector and control method thereof - Google Patents
Bioluminescence detector and control method thereof Download PDFInfo
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- CN104007105A CN104007105A CN201410245378.8A CN201410245378A CN104007105A CN 104007105 A CN104007105 A CN 104007105A CN 201410245378 A CN201410245378 A CN 201410245378A CN 104007105 A CN104007105 A CN 104007105A
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- syringe
- control module
- sliding panel
- detection device
- photomultiplier
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Abstract
The invention discloses a bioluminescence detector and a control method thereof, wherein two injectors are respectively used for extracting a target sample and a standard sample; because microorganisms in the samples emit fluorescent light, two photomultipliers are respectively used for detecting the fluorescent light in the two injectors, and then two groups of electrical signals are sent to a control unit, the control unit works out light emitting value data, and the bioluminescence detector is used for testing the samples by adopting a dual-channel comparison manner. Compared with a conventional manually sampling and manual testing manner, the bioluminescence detector has the advantages of having automation performance, preventing errors caused by manual operation, and improving the detection precision and the data reliability.
Description
Technical field
The present invention relates to water quality detection equipment, relate in particular to a kind of bioluminescent detection device and control method thereof.
Background technology
Along with industrial expansion and scientific and technological progress, the quantity of chemical plant and related application enterprise is in continuous growth.In recent years, the chemical products that cause due to factors such as miscarriage, disasteies and industrial wastewater discharge, in rivers, cause water quality toxicity to strengthen, harm people's life safety.In water quality on-line detecting system, need to carry out bio-toxicity detection to the river that has chemical products to poison, in water quality detection equipment, conventionally adopt two plastic injectors respectively to target water sample with sample with reference to water sample, recycling photomultiplier is aimed at the cavity of syringe, detect target sample and with reference to the fluorescence intensity data of microorganism in water sample, according to the contrast of two groups of data, the detection of realization to target water quality toxicity, this kind of detection mode plays a significant role in water quality detection work, but, in above-mentioned water quality detection equipment, need user manually to extract sample and manual fluorescence intensity, the robotization poor-performing that causes checkout equipment, detect data precision not high, poor reliability.
Summary of the invention
The technical problem to be solved in the present invention is, for the robotization poor-performing of existing water quality detection equipment, detect the deficiencies such as the not high and poor reliability of data precision, provides a kind of bioluminescent detection device and control method thereof.
For solving the problems of the technologies described above, the present invention adopts following technical scheme.
A kind of bioluminescent detection device, it includes shell, described shell is provided with stepper motor, control module and two photomultipliers, in described shell, be provided with the screw mandrel of vertical extension, the sliding panel that can slide up and down and two syringes, described sliding panel is fixedly connected with the nut seat on screw mandrel, top and this stepper motor that described stepper motor is positioned at shell drive screw mandrel to rotate, the transfusion end of described syringe is through shell, the piston end of described syringe is fixedly connected with sliding panel, described stepper motor and photomultiplier are all electrically connected on control module, described photomultiplier is corresponding one by one with described syringe, the test side of described photomultiplier is towards the cavity of syringe, described photomultiplier, for detection of the fluorescence in syringe, is converted to electric signal by fluorescence signal, and transfers to control module after this electric signal is amplified, two groups of electric signal that described control module sends two photomultipliers contrast, and calculate luminous value data.
Preferably, described shell is provided with at least one gate controlled switch, the front panel of described gate controlled switch and shell offsets, and this gate controlled switch is electrically connected to control module, and described control module is controlled photomultiplier according to the on/off state of gate controlled switch and powered on/stop.
Preferably, the outside surface of described piston is coated with black light-absorbing material.
Preferably, described photomultiplier is near the cavity lower end of syringe, and the cavity upper end of described syringe is arranged with shading sleeve.
Preferably, described syringe is glass syringe.
Preferably, the two ends of described sliding panel are equipped with respectively a feed rod, and described feed rod is parallel with described screw mandrel, and described sliding panel slides up and down along described feed rod.
A kind of control method of bioluminescent detection device as above, it comprises the steps: step S1, the transfusion end-grain cutting of described syringe is changed and is connected to sample cell, described control module control step motor forward running, described screw mandrel drives sliding panel upward sliding, described sliding panel drives piston to move upward, and makes described syringe extract sample; Step S2, described photomultiplier detects the fluorescence in syringe, and fluorescence signal is converted to electric signal, and transfers to control module after this electric signal is amplified; Step S3, two groups of electric signal that described control module sends two photomultipliers contrast, and calculate luminous value data; Step S4, described control module control step motor antiport, described screw mandrel drives sliding panel down sliding, and described sliding panel drives piston to move downward, and makes described syringe drain sample.
Preferably, also include: step S5, the transfusion end-grain cutting of described syringe is changed and is connected to service sink, described control module control step motor forward running, described screw mandrel drives sliding panel upward sliding, and described sliding panel drives piston to move upward, and makes described syringe extract clear water and cleans.
In bioluminescent detection device disclosed by the invention and control method thereof, two syringes are respectively used to extracting objects sample and standard sample, because the microorganism in sample sends fluorescence, so respectively the fluorescence in two syringes is detected by two photomultipliers, afterwards two groups of electric signal are sent to control module, by control module, calculate luminous value data, if the luminous value of target sample is larger compared with the luminous value of standard sample, illustrate that the micro organism quantity in target sample is more, the pollution of target sample is more serious.This bioluminescent detection device is tested sample in the mode of binary channels contrast, compare traditional artificial extraction sample, manual testing's mode, the present invention has more robotization performance, and has avoided the error that causes because of manual operation, has improved the reliability of accuracy of detection and data.
Accompanying drawing explanation
Fig. 1 is the cut-away view of biological luminescence detector.
Fig. 2 is the side view of biological luminescence detector.
Embodiment
Below in conjunction with drawings and Examples, the present invention is done to more detailed description.
The invention discloses a kind of bioluminescent detection device, shown in Fig. 1 and Fig. 2, it includes shell 1, described shell 1 is provided with stepper motor 2, control module 6 and two photomultipliers 7, in described shell 1, be provided with the screw mandrel 3 of vertical extension, the sliding panel 4 that can slide up and down and two syringes 5, sliding panel 4 is fixedly connected with the nut seat 31 on screw mandrel 3, top and this stepper motor 2 that described stepper motor 2 is positioned at shell 1 drive screw mandrel 3 to rotate, the transfusion end 50 of described syringe 5 is through shell 1, the piston end of described syringe 5 is fixedly connected with sliding panel 4, described stepper motor 2 and photomultiplier 7 are all electrically connected on control module 6, described photomultiplier 7 is corresponding one by one with described syringe 5, the test side of described photomultiplier 7 is towards the cavity of syringe 5, described photomultiplier 7, for detection of the fluorescence in syringe 5, is converted to electric signal by fluorescence signal, and transfers to control module 6 after this electric signal is amplified, two groups of electric signal that described control module 6 sends two photomultipliers 7 contrast, calculate luminous value data, if the luminous value of target sample is larger compared with the luminous value of standard sample, the micro organism quantity in target sample is more, and the pollution of target sample is more serious.
In above-mentioned bioluminescent detection device, the operating condition of control module 6 control step motors 2, when stepper motor 2 forward running, screw mandrel 3 drives sliding panel 4 upward slidings by screw pair 31, simultaneously, syringe 5 extracts sample because piston 51 moves upward, two syringes 5 are respectively used to extracting objects sample and standard sample, because the microorganism in sample sends fluorescence, so respectively the fluorescence in two syringes 5 is detected by two photomultipliers 7, afterwards two groups of electric signal are sent to control module 6, by control module 6, calculate luminous value data, this bioluminescent detection device is tested sample in the mode of binary channels contrast, compare traditional artificial extraction sample, manual testing's mode, the present invention has more robotization performance, and avoided the error causing because of manual operation, improved the reliability of accuracy of detection and data.
Because photomultiplier 7 is for detection of low-lying level light; so be loaded with the photomultiplier 7 of voltage easily damages under natural light irradiation; for fear of damaging photomultiplier 7; described shell 10 is provided with at least one gate controlled switch 10; described gate controlled switch 10 offsets with the front panel of shell 1; and this gate controlled switch 10 is electrically connected to control module 6, described control module 6 is controlled photomultiplier 7 according to the on/off state of gate controlled switch 10 and is powered on/stop, thus protection photomultiplier 7.
Adjacent for fear of two pistons 51 produces luminous interference, the outside surface of described piston 51 is coated with black light-absorbing material, and described photomultiplier 7 is near the cavity lower end of syringe 5, the cavity upper end of described syringe 5 is arranged with shading sleeve 9, make the fluorescence of sample within syringe 5 only can conduct to photomultiplier 7, avoid because of light disturbing effect measuring accuracy.
In prior art, adopt plastic injector to extract sample, yet plastic injector exists the defects such as transparency is bad, in order to improve translucent effect, syringe 5 is preferably glass syringe more.
In order to realize sliding panel 4, slide up and down, the two ends of described sliding panel 4 are equipped with respectively a feed rod 11, and described feed rod 11 is parallel with described screw mandrel 3, and described sliding panel 4 slides up and down along described feed rod 11.
This bioluminescent detection device in actual applications, can coordinate preset sample cell and service sink to test and clean, and is loaded with sample in sample cell, is loaded with clear water in service sink.
On the basis of above-mentioned bioluminescent detection device structure, the invention also discloses the control method of this bioluminescent detection device, the method comprises the steps:
Step S1, the transfusion end-grain cutting of described syringe 5 is changed and is connected to sample cell, described control module 6 control step motor 2 forward runnings, and described screw mandrel 3 drives sliding panel 4 upward slidings, described sliding panel 4 drives piston 51 to move upward, and makes described syringe 5 extract sample;
Step S2, the fluorescence that described photomultiplier 7 detects in syringe 5, is converted to electric signal by fluorescence signal, and transfers to control module 6 after this electric signal is amplified;
Step S3, two groups of electric signal that described control module 6 sends two photomultipliers 7 contrast, and calculate luminous value data;
Step S4, described control module 6 control step motor 2 antiports, described screw mandrel 3 drives sliding panel 4 down slidings, and described sliding panel 4 drives piston 51 to move downward, and makes described syringe 5 drain samples.
Step S5, the transfusion end-grain cutting of described syringe 5 is changed and is connected to service sink, described control module 6 control step motor 2 forward runnings, and described screw mandrel 3 drives sliding panel 4 upward slidings, described sliding panel 4 drives piston 51 to move upward, and makes described syringe 5 extract clear water and cleans.
In said process, the work such as the extraction of sample, detection, cleaning all complete automatically, there is good robotization performance, and realized the sampling of binary channels equivalent, by contrast test, draw the pollution level of target sample, in conjunction with above, what time can find out, bioluminescent detection device disclosed by the invention and control method thereof are for river, river, lake, enterprise's discharge water etc., to carry out the ideal tools of science detection.
The above is preferred embodiment of the present invention, is not limited to the present invention, all modifications of making in technical scope of the present invention, is equal to and replaces or improvement etc., all should be included in the scope that the present invention protects.
Claims (8)
1. a bioluminescent detection device, it is characterized in that, include shell, described shell is provided with stepper motor, control module and two photomultipliers, in described shell, be provided with the screw mandrel of vertical extension, the sliding panel that can slide up and down and two syringes, described sliding panel is fixedly connected with the nut seat on screw mandrel, top and this stepper motor that described stepper motor is positioned at shell drive screw mandrel to rotate, the transfusion end of described syringe is through shell, the piston end of described syringe is fixedly connected with sliding panel, described stepper motor and photomultiplier are all electrically connected on control module, described photomultiplier is corresponding one by one with described syringe, the test side of described photomultiplier is towards the cavity of syringe,
Described photomultiplier, for detection of the fluorescence in syringe, is converted to electric signal by fluorescence signal, and transfers to control module after this electric signal is amplified;
Two groups of electric signal that described control module sends two photomultipliers contrast, and calculate luminous value data.
2. bioluminescent detection device as claimed in claim 1, it is characterized in that, described shell is provided with at least one gate controlled switch, the front panel of described gate controlled switch and shell offsets, and this gate controlled switch is electrically connected to control module, described control module is controlled photomultiplier according to the on/off state of gate controlled switch and is powered on/stop.
3. bioluminescent detection device as claimed in claim 1, is characterized in that, the outside surface of described piston is coated with black light-absorbing material.
4. bioluminescent detection device as claimed in claim 1, is characterized in that, described photomultiplier is near the cavity lower end of syringe, and the cavity upper end of described syringe is arranged with shading sleeve.
5. bioluminescent detection device as claimed in claim 1, is characterized in that, described syringe is glass syringe.
6. bioluminescent detection device as claimed in claim 1, is characterized in that, the two ends of described sliding panel are equipped with respectively a feed rod, and described feed rod is parallel with described screw mandrel, and described sliding panel slides up and down along described feed rod.
7. as a control method for the bioluminescent detection device of claim 1 to 6 as described in arbitrarily, it is characterized in that, comprise the steps:
Step S1, the transfusion end-grain cutting of described syringe is changed and is connected to sample cell, described control module control step motor forward running, described screw mandrel drives sliding panel upward sliding, and described sliding panel drives piston to move upward, and makes described syringe extract sample;
Step S2, described photomultiplier detects the fluorescence in syringe, and fluorescence signal is converted to electric signal, and transfers to control module after this electric signal is amplified;
Step S3, two groups of electric signal that described control module sends two photomultipliers contrast, and calculate luminous value data;
Step S4, described control module control step motor antiport, described screw mandrel drives sliding panel down sliding, and described sliding panel drives piston to move downward, and makes described syringe drain sample.
8. the control method of bioluminescent detection device as claimed in claim 7, is characterized in that, also includes:
Step S5, the transfusion end-grain cutting of described syringe is changed and is connected to service sink, described control module control step motor forward running, described screw mandrel drives sliding panel upward sliding, and described sliding panel drives piston to move upward, and makes described syringe extract clear water and cleans.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410245378.8A CN104007105A (en) | 2014-06-04 | 2014-06-04 | Bioluminescence detector and control method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410245378.8A CN104007105A (en) | 2014-06-04 | 2014-06-04 | Bioluminescence detector and control method thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| EP1884766A1 (en) * | 2005-05-20 | 2008-02-06 | Hitachi Chemical Company, Ltd. | Method of analyzing biochemical |
| WO2008048371A2 (en) * | 2006-04-04 | 2008-04-24 | Singulex, Inc. | Methods and compositions for highly sensitive analysis of markers and detection of molecules |
| CN201834917U (en) * | 2010-04-27 | 2011-05-18 | 杭州绿洁水务科技有限公司 | Absorption and desorption device for comprehensive toxicity detection |
| CN102445446A (en) * | 2011-09-28 | 2012-05-09 | 程澎 | Chemiluminescence immunodetection method and device |
| CN202256163U (en) * | 2011-09-26 | 2012-05-30 | 江南大学 | Portable pesticide residual toxicity photoelectric fast detector |
| CN102967588A (en) * | 2012-10-24 | 2013-03-13 | 吉林化工学院 | Formaldehyde fluorescence detector |
-
2014
- 2014-06-04 CN CN201410245378.8A patent/CN104007105A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1884766A1 (en) * | 2005-05-20 | 2008-02-06 | Hitachi Chemical Company, Ltd. | Method of analyzing biochemical |
| WO2008048371A2 (en) * | 2006-04-04 | 2008-04-24 | Singulex, Inc. | Methods and compositions for highly sensitive analysis of markers and detection of molecules |
| WO2008048371A3 (en) * | 2006-04-04 | 2008-10-16 | Singulex Inc | Methods and compositions for highly sensitive analysis of markers and detection of molecules |
| CN201834917U (en) * | 2010-04-27 | 2011-05-18 | 杭州绿洁水务科技有限公司 | Absorption and desorption device for comprehensive toxicity detection |
| CN202256163U (en) * | 2011-09-26 | 2012-05-30 | 江南大学 | Portable pesticide residual toxicity photoelectric fast detector |
| CN102445446A (en) * | 2011-09-28 | 2012-05-09 | 程澎 | Chemiluminescence immunodetection method and device |
| CN102967588A (en) * | 2012-10-24 | 2013-03-13 | 吉林化工学院 | Formaldehyde fluorescence detector |
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Application publication date: 20140827 |