CN104007056A - Ultraviolet ray damage resistance evaluating method - Google Patents
Ultraviolet ray damage resistance evaluating method Download PDFInfo
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- CN104007056A CN104007056A CN201410008695.8A CN201410008695A CN104007056A CN 104007056 A CN104007056 A CN 104007056A CN 201410008695 A CN201410008695 A CN 201410008695A CN 104007056 A CN104007056 A CN 104007056A
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Abstract
The invention aims to provide an ultraviolet ray damage resistance evaluating index using a skin horny layer acquired in a non-invasive way. A tape stripping method is adopted to acquire the skin horny layer. The DJ-1 protein expression quantity in the horny layer is measured as the index which is further adopted in the ultraviolet ray damage resistance evaluating method.
Description
Technical field
The present invention relates to the evaluation method to the repellence of ultraviolet injury.
Background technology
Ultraviolet injury is to work as when irradiated ultraviolet ray surpasses melanic protective capability to cause.The phenomenon of ultraviolet injury has 2 kinds, just after irradiation ultraviolet radiation, there is not symptom, erythrosis, sunburn (erythema: in sunburn) He 24～72 hours, pigmentation continues tanned (suntan) that carry out that after 6～48 hours, pain becomes the strongest after 2～6 hours.Sunburn refers to that UVB sees through the through papilla body (mammillary body) of epidermis, and the capillary in result corpora mammillare occurs congested as inflammatory reaction, the state that the color of skin reddens (erythema).When amount of ultraviolet surpasses melanic defense reaction, cell tissue sustains damage, and causes heating, bubble, pain.Be referred to as solar dermatitis (solar dermatitis).
In addition, tanned is that UVA promotes melanocyte (melanocyte) work, thereby promotes melanic generation.UVA does not follow rubescent, inflammation, due to the metabolism of skin is slowed down, produces spot.
Fears are entertained that, and these ultraviolet ray meetings that become the root of ultraviolet injury produce multiple impact to health, as on the carcinogenesis of skin, on the impact of the eye disease of cataract etc., on impact of immunologic function etc.When skin is subject to ultraviolet ray and is exposed to the sun, can cause various ultraviolet injury.Ultraviolet ray makes skin produce inflammation, or makes the unsaturated lipids of skin be oxidized and produce lipid free radical, thereby generates lipid peroxide.This damage because of UV-induced inflammation, oxidation meeting initiation tissue, cell, DNA.The phenomenon producing as the UV light exposure because of skin, can enumerate wrinkle, spot, cutaneum carcinoma etc. conventionally.
In recent years, as epidemiology (Epidemiology) data, also with good grounds to the difference of ultraviolet susceptibility (color of skin, the type of skin etc.) and the report of the increased risk of cutaneum carcinoma.And then, show the dangerous risk as a kind of malignant mela noma (melanoma) of cutaneum carcinoma, the caused increased risk several times of gene mutation that replace because of 1 base of Melanocortins-1 acceptor (melanocortin1receptor).Ultraviolet susceptibility is differed widely according to the difference of skin type (sunburn when being subject to ultraviolet injury, tanned state is different classify), or divide into the dissimilar of 6 grades, or take the minimum ultraviolet energy (minimal erythema dose: MED) as standard is classified of general red (erythema) occurs, the former is subjective observation, and the latter follows not irradiation ultraviolet radiation with regard to unmeasured risk.Due to depletion of the ozone layer etc., become more serious problem with the ultraviolet ray increase of comparing in the past, so need to suitably evaluate ultraviolet susceptibility risk for individual.
On the other hand, can form the Pigmented starting stage that is commonly called spot, before skin surface obviously occurs due to various stimulations, the information transmitter substance of keratinization of epidermis Hemapoiesis α-MSH, bFGF, CGRP, Endothelin etc., then pass to melanocyte.After passing to melanocyte, in cell, form a large amount of melanin.And then because ultraviolet turnover (turnover) occurs extremely, the melanin that causes a large amount of formation cannot be discharged and be changed pigment venereal disease into and become.It is reported in using the experiment of vitro system, when melanocyte is irradiated to UV, as p73, the Nup88 of growth factor label, p27, Id1, PCNA and as the expression of the bcl-2 of Apoptosis related protein matter, increase and decrease (non-patent literature 1).
Up to the present, it is reported use by bioptic skin histology invasion and attack formula spot portion and normal portion are contrasted, the protein expressions such as the NT-3 of spot portion, ADAM9, HB-EGF significantly increase (patent documentation 1).And then, it is reported the inspection method forming as prediction skin spot, the mRNA that can measure MLSTD1, MOGAT1, Mcp9, Krt2-6b etc. by biopsy measures, thus the risk (patent documentation 2,3,4) that judgement spot forms.In addition, method as non-invasion and attack formula, in patent documentation 5, disclose using the cuticula from skin gathering via tape stripping or friction as sample, analyzed the amount of interleukin-11 (IL-1) and interleukin 1 receptor antagonist (IL-1ra), thereby evaluated the method for myoplasm.
In addition, the applicant has proposed following patented claim, from use the keratoderma of method collection of non-invasion and attack formula, detect annexin I I, BLMH, cathepsin D, arginase-1, SCCA2, as to the technology of the index of the repellence of skin sunburn (patent documentation 6).In addition, following patented claim has also been proposed, from use the keratoderma of method collection of non-invasion and attack formula, detect BLMH, annexin I I, cathepsin D, arginase-1, beta-actin, keratin (keratin) 14, Keratin 1, SCCA2, as the technology (patent documentation 7) of the index of pigmentation trend.
In addition it is useful so proposed patented claim (patent documentation 8) as atopic dermatitis marker that, the applicant also finds to have the DJ-1 protein (another name PARK-7 protein) of antioxidation.In addition, show gradually that in recent years the label that this DJ-1 protein can be used as various diseases utilizes.Patent documentation 9 discloses using oxidized form DJ-1 protein as specific recognition antibody.Secondly, proposed by coming diagnosis of alzheimer's disease, Parkinsonian method with this TPPA oxidized form DJ-1.In addition, patent documentation 10 discloses the method for diagnosing brain damage disease by measuring the DJ-1 protein of cerebrospinal fluid, blood etc.In addition, patent documentation 11 has proposed to come by measuring DJ-1 protein method and the reagent of diagnosing diabetic retinopathy.
Prior art document
Patent documentation
Patent documentation 1 TOHKEMY 2004-205246 communique
Patent documentation 2 TOHKEMY 2005-106745 communiques
Patent documentation 3 TOHKEMY 2005-110505 communiques
Patent documentation 4 TOHKEMY 2007-289063 communiques
No. 3590708 communique of patent documentation 5 Jap.P.s
Patent documentation 6 TOHKEMY 2010-151482 communiques
Patent documentation 7 TOHKEMY 2009-008607 communiques
Patent documentation 8 WO2007/046463 communiques
Patent documentation 9 TOHKEMY 2010-183843 communiques
Patent documentation 10 WO2007/060886 communiques
Patent documentation 11 TOHKEMY 2009-168819 communiques
Non-patent literature
Non-patent literature 1Carcinogenesis24(12): 1929-1934,2003
Summary of the invention
Problem of the present invention is to use the keratoderma with the method collection of non-invasion and attack formula, and the evaluation index to the repellence of ultraviolet injury is provided.And the repellence to ultraviolet injury of mentioning in the present invention refer to by UV light exposure, caused rubescent at 15 days with interior recovery, and the lightness of skin is also recovered together.
The present application person finds in the process of research people sunburn, uses the DJ-1 protein of people's keratoderma that the method for non-invasion and attack formula obtains by mensuration, can evaluate the repellence to ultraviolet injury.The present invention is based on this achievement in research.
(1) evaluation method to the repellence of ultraviolet injury, is characterized in that, measures the DJ-1 protein expression amount of keratoderma.
(2) according to the evaluation method (1) described, it is characterized in that possessing following operation: the cuticular collection operation at collection experimenter's evaluation object position, mensuration are by the mensuration operation of the DJ-1 expression in the cuticula of described collection operation collection, the contrast operation that the expression of the described evaluation object cuticular DJ-1 in position of the DJ-1 protein expression amount of measuring by described mensuration operation and sample group is distributed and contrasted.
(3) according to the evaluation method (2) described, it is characterized in that, the operation that gathers keratoderma is undertaken by tape stripping method.
According to the present invention, need not actual irradiation ultraviolet radiation, can evaluate the repellence of people to ultraviolet injury.In addition, because not being uses biopsy etc. to the influential method of human body, but use the keratoderma of the tape stripping method collection of non-invasion and attack formula to evaluate, so safe and easy.
In addition, according to the present invention, also can evaluate the restoring force by UV-induced inflammation (erythema).
In addition, owing to inferring when the restoring force by UV-induced inflammation is low, the risk of the skin aging being caused by UV light exposure, wrinkle, spot and then cutaneum carcinoma is high, so to the people a little less than ultraviolet injury repellence, can positively recommend to use in advance the beauty treatment consulting service of potent suncream etc.
Accompanying drawing explanation
Fig. 1 is for analyzing " general red " degree after 15 days of the skin that irradiates through ultraviolet ray and the graph of a relation of DJ-1 expression.
Fig. 2 is for analyzing " whiting " degree of skin after 15 days and the graph of a relation of DJ-1 expression irradiating through ultraviolet ray.
Fig. 3 is for representing carry out the rheological parameters' change with time figure of Δ a* value of conduct " general red " index of ultraviolet postradiation skin according to the experimenter of DJ-1 initial value classification.
Fig. 4 is for representing carry out the rheological parameters' change with time figure of Δ L* value of conduct " whiting " index of ultraviolet postradiation skin according to the experimenter of DJ-1 initial value classification.
Fig. 5 is for representing 716 adults' of random selection the distribution of DJ-1 measured value and the figure of cumulative number.The left side longitudinal axis represents number, and the right side longitudinal axis represents cumulative percentage.
Embodiment
Below, for embodiments of the present invention, illustrate in further detail.
Evaluation method of the present invention is characterised in that, using the amount of the DJ-1 protein in keratoderma as index.
DJ-1 protein, also referred to as PARK7, is molecular weight 19, the intracellular protein of 891Da.DJ-1 protein is accredited as the protein relevant with Parkinson's, shows that afterwards it is relevant with the re-epithelialization (re-epithelialization) in skin wound healing process.In addition, from its spatial structure prompting, also can be used as proteinase plays a role.
Keratoderma is the tissue that is positioned at the skin the superiors, has the effect that protection skin is not subject to exogenic foreign matter, stimulation.
Evaluation object position in the present invention so long as can obtain cuticular part, can comprise any part, as main position, can enumerate face, neck, upper arm parts.According to method in the past, can obtain the cuticula from the skin at these positions.And the method for winning skin etc. due to surgery can be brought burden to user, therefore preferably adopt tape stripping, friction etc. can obtain easily cuticular method during the invention process.Tape stripping method particularly preferably.
The DJ-1 protein expression amount of each sample can be measured by the past known method.For example, reacting of the antibody based on DJ-1 protein, can be used the method for enzyme immunoassay (EIA), radio immunoassay, immunoblotting etc.
Himself known biochemical method for sample by DJ-1 protein from gathering, for example freeze-thaw method, ultrasonic fragmentation, homogenate method are prepared soluble component.Fast Measurement DJ-1 after extraction soluble component.
Secondly, the people that DJ-1 protein expression amount is many is evaluated as the repellence of ultraviolet injury strong.
In addition, the measurement result based on DJ-1, can instruct the people who should be noted that sunburn to note outdoor activity.Or, from the measurement result of DJ-1, be judged as when low to the repellence of ultraviolet injury, can take separately to select the countermeasure of cosmetics that ultraviolet screening effect is strong etc.
Secondly, the consulting service that the cosmetics that in the past carried out with ambiguous standard are selected can have science according to correctly carrying out.
The method safety of the present application by non-invasion and attack formulas such as tape strippings and easily gather keratoderma, can be used this keratoderma to measure at short notice the expression of specified protein.Therefore, can measure simply by toilet articles counter etc., the on-the-spot information of selecting as cosmetics is applied.Based on this result, also can represent applicable cosmetics.
For implementing the mensuration of the collection of keratoderma of the present invention and the extraction of protein, DJ-1 protein expression amount, all can implement by following operation.
(1) cuticula by tape stripping method gathers
Tape stripping method refers to tape-stripping in skin, is attached to the cuticular method of bonding plane by stripping tape collection.
Can use commercially available adhesive tape, for example, by adhesive tape being pressed on to people's cheek portion, make cuticula be attached to adhesive tape collection.As commercially available adhesive tape, can enumerate ASAHIBIOMED company cuticula test pieces processed, MORITEX company cuticula envelope processed paper (seal), PROMOTOOL company cuticula test pieces processed (collar plate shape W, collar plate shape G, PRO type), the Corneofix processed of Integral company, 3M company adhesive tape processed, 3M company transparent double face glue processed etc.
(2) from cuticula, carry out the damping fluid that Protein Extraction is used
In order to extract cuticula protein use Extraction buffer from being attached to the cuticula of adhesive tape.As Extraction buffer, such as can illustration PBS(containing 0.1%SDS) etc.
(3) extraction of cuticula protein
The adhesive tape that cuticula is adhered to is put into cylindrical vessel, by homogenizing with pestle (hereinafter referred to as " the pestle ") bonding plane that rubs of use High Rotation Speed, can promptly extract cuticula protein.The bonding plane of adhesive tape is towards cylindrical vessel inner side, and the opposing face of bonding plane is put into cylindrical vessel along the inner side of cylindrical vessel.By the face of the bonding plane opposition side with adhesive tape is put into along the inner side of cylindrical vessel, the available pestle bonding plane that easily rubs.
Or the adhesive tape that also cuticula can be adhered to immerses Extraction buffer, extracts cuticula protein with scraper friction, also can use succusion, supercritical ultrasonics technology.Or, while also can be with beaded glass the fragmentation of vibrating of the device by eddy blending machine etc. extract.
(4) recovery of cuticula extracting solution of protein
By standing approximately 1 minute of cuticula extracting solution of protein, make foam calmnessization, thereafter, from cylindrical vessel, draw cuticula extracting solution of protein and reclaim.
(5) the DJ-1 protein expression quantitative analysis in cuticula protein
DJ-1 protein in the cuticula extracting solution of protein obtaining can carry out quantitative test by known methods such as Western blot, ELISA method, antibody chip method, FRET methods.
Below, embodiment is shown and further illustrates the present invention.
Embodiment
1. the mensuration that the DJ-1 albumen quality of pair repellence that ultraviolet ray is irradiated and keratoderma changes
19 men and women experimenters' of investigation irradiates the general red variation of skin causing, the degree of irradiating rear recovery and the relation of DJ-1 protein by ultraviolet ray.
The observation > of the skin condition after < ultraviolet ray irradiation and pre-irradiation
In the Medial upper arm portion with 19 men and women of healthy skin, use ultraviolet lamp (DERMARAY, Terumo Clinical Supply Co., Ltd. system), with 60mJ/cm2(1cm * 1cm) carry out irradiation ultraviolet radiation.Ultraviolet ray pre-irradiation and measure skin color with spectral photometric colour measuring meter (SM-500, Konica Minolta company system) after 1,5,8,15 day, observes skin condition.
In addition, at ultraviolet pre-irradiation and irradiate after 8 days, use cuticula test pieces
(ASAHIBIOMED company) gathers cuticula by tape stripping method.
The mensuration > of the DJ-1 protein expression amount in < cuticula
At the test tube of having put into beaded glass and 500 μ l T-PER damping fluids (Thermo scientific), put into and gathered cuticular cuticula test pieces, with eddy blending machine vibration, within 25 minutes, extract cuticula protein.BCA protein Assay Kit(Thermo Scientific for the protein content of each sample) measure.In cuticula extract, contained DJ-1 albumen quality is used HumanPark7/DJ-1 3-protein d uoSet(R & D systems company system) carry out quantitatively.Protein determination, DJ-1 protein measuring carry out according to the determination experiment guide of kit.DJ-1 albumen quality represents by the DJ-1 amount (pg/ μ g protein) of every 1 μ g protein.
The analysis > of < measurement result
(1) follow general red (rubescent) variation of ultraviolet ray irradiation and the relationship analysis of DJ-1 albumen quality
According to the Δ a* value of irradiating the general red index of the 15th day as ultraviolet ray, be divided into following 2 groups.
" the general red recovery that do not have ": the 15th day Δ a* value is more than 1 experimenter (7)
" general red recovery ": the experimenter (12) that the 15th day Δ a* value is less than 1
And the experimenter who is evaluated as " general red recovery " has ultraviolet repellence.
The DJ-1 protein measuring result of " the general red recovery that do not have " group:
DJ-1 protein median: 1.60pg/ μ g protein, mean value: 1.52pg/ μ g protein
The DJ-1 protein measuring result of " general red recovery " group:
DJ-1 protein median: 2.59pg/ μ g protein, mean value: 2.66pg/ μ g protein
Significant difference check between two groups is used graceful-Whitney (Mann – Whitney) U method of inspection to carry out.
There is significant difference in the DJ-1 protein measuring value of two groups, the experimenter's of " general red recovery " group DJ-1 protein is compared and demonstrated high value with the experimenter of " the general red recovery that do not have " group.Its boundary value is 2pg/ μ g protein left and right.The contrast of the DJ-1 albumen quality of two groups and assay are as shown in Figure 1.
(2) according to ultraviolet ray, irradiate after " whiting " and the analysis of recovery extent
According to the Δ L* value of the 15th day, be divided into following 2 groups.
Compare with ultraviolet pre-irradiation " whiting not have recovery ": the experimenter (6) that the 15th day Δ L* value is less than-1
Compare " whiting recovers " with ultraviolet pre-irradiation: the 15th day Δ L* value is more than-1 experimenter (13)
And the experimenter who is evaluated as " whiting recovers " has ultraviolet repellence.
" whiting does not recover ": DJ-1 protein median: 1.59pg/ μ g protein, mean value: 1.46pg/ μ g protein
" whiting recovery ": DJ-1 protein median: 2.11pg/ μ g protein, mean value: 2.60pg/ μ g protein
Significant difference check between two groups is used Mann Whitney U test method to carry out.
There is significant difference in the DJ-1 protein measuring value of two groups, the experimenter's of " whiting recovers " group DJ-1 protein is compared and demonstrated high value with the experimenter of " whiting does not have to recover " group.Its boundary value is 2pg/ μ g protein left and right.The contrast of the DJ-1 albumen quality of two groups and assay are as shown in Figure 2.
From the analysis result of above 1.2., DJ-1 protein expression amount can become from the index of the recovery capability of sunburn.
(3) according to " general red " of DJ-1 protein initial value, the analysis of " whiting "
The DJ-1 albumen quality (initial value) of irradiating the 0th day according to ultraviolet ray is divided into upper 30%, the next 30% experimenter, comparative analysis " general red " with " whiting " and state.The DJ-1 protein initial value that is categorized as 6 experimenters of " DJ-1 protein is few " is distributed in 0.65-1.59pg/ μ g protein.
On the other hand, the DJ-1 protein initial value that is categorized as 6 experimenters of " DJ-1 protein is many " is distributed in 2.87-4.85pg/ μ g protein.
Represent ultraviolet postradiation skin " general red " Δ a* variation as shown in Figure 3.Can confirm that ultraviolet ray irradiation is after 15 days, the high group of initial value of DJ-1 represents that the Δ a* value of " general red " is reduced to 0, is restored from rubescent.On the other hand, the group ultraviolet ray that the initial value of DJ-1 is low is irradiated Δ a* value after 15 days and is not almost reduced.The general red difference of the group that the ultraviolet ray irradiation DJ-1 of the 15th day is high and low group is significantly upper in 5% level of significance (significance level) with Mann Whitney U test.Hence one can see that, and the high group of initial value of DJ-1 has the restoring force from ultraviolet injury.
Represent ultraviolet postradiation skin " whiting " Δ L* variation as shown in Figure 4.As shown in Figure 4, skin after 24 hours is irradiated in ultraviolet ray " whiting " the degree high group group low with the initial value of DJ-1 of initial value that be DJ-1 compare and demonstrate high value.Hence one can see that, and the group group lower than DJ-1 that DJ-1 is high has more ultraviolet repellence.
In addition, can confirm that ultraviolet ray irradiation is after 15 days, the high group of initial value of DJ-1 represents that the Δ L* value of " whiting " rises and roughly returns to initial value.On the other hand, the group that the initial value of DJ-1 is low Δ L* value after ultraviolet ray is irradiated 15 days does not almost change.The difference of the Δ L* of the group that the ultraviolet ray irradiation DJ-1 of the 15th day is high and low group is remarkable in 5% level of significance with Mann Whitney U test.Hence one can see that, and the high group of initial value of DJ-1 has the restoring force from ultraviolet injury.
From the postradiation skin of above ultraviolet ray " general red " change, " whiting " change and the analysis result of DJ-1 initial value, using DJ-1 as index, can evaluate the repellence of the skin of ultraviolet injury and restorative.In addition the 2pg/ μ g protein left and right of the boundary value of, predicting its repellence when using DJ-1 as index.
2. the distribution of the DJ-1 albumen quality of 205 of random selection and ultraviolet injury repellence is pre-
survey
(1) collection of test sample
Using 205 women of random selection as object, from cheek, by tape stripping method, gather cuticula sample.Sample is that 1 position gathers 1.
(2) DJ-1 in cuticula measures
At the test tube of having put into beaded glass and 500 μ l T-PER damping fluids (Thermo scientific company), put into and gathered cuticular cuticula test pieces, with eddy blending machine vibration, within 25 minutes, extract cuticula protein.BCA protein Assay Kit(Thermo Scientific company for the protein content of each sample) measure.Be determined as and in 10 μ l cuticula samples, add 200 μ l according to reagent A: the liquid that reagent B=50:1 is mixed, at 60 ℃, hatch after 30 minutes, measure the absorbance at 562nm place.Meanwhile, with bovine serum albumin(BSA) (BSA) production standard curve.From the value of this typical curve and absorbance, calculate protein content.
The DJ-1 amount comprising in cuticula extract is used Human Park7/DJ-1DuoSet(R & D systems company) carry out quantitatively.Measurement result is shown in to Fig. 5 as take number and the accumulative total rate that 0.5pg/ μ g protein is unit.
Expression in the cuticula of the DJ-1 protein of 205 of randomly drawing as shown in Figure 5, is distributed in the wide scope from 0.29pg/ μ g protein to 8.8pg/ μ g protein.Mean value is 2.6pg/ μ g protein.
During boundary value using the 2pg/ μ g protein of predicting by 1 test as ultraviolet injury repellence, predict that the repellence of experimenter's ultraviolet injury of approximately 40% is low.
Claims (3)
1. the evaluation method to the repellence of ultraviolet injury, is characterized in that, measures the DJ-1 protein expression amount of keratoderma.
2. evaluation method according to claim 1, it is characterized in that possessing following operation: the cuticular collection operation at collection experimenter's evaluation object position, mensuration are by the mensuration operation of the DJ-1 expression in the cuticula of described collection operation collection, the contrast operation that the expression of the described evaluation object cuticular DJ-1 in position of the DJ-1 protein expression amount of measuring by described mensuration operation and sample group is distributed and contrasted.
3. evaluation method according to claim 2, is characterized in that, the operation that gathers keratoderma is undertaken by tape stripping method.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2013032745A JP5775538B2 (en) | 2013-02-22 | 2013-02-22 | Evaluation method of resistance to redness (redness) generation and reduction of whiteness induced by UV exposure |
| JP2013-032745 | 2013-02-22 |
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| Publication Number | Publication Date |
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| CN104007056A true CN104007056A (en) | 2014-08-27 |
| CN104007056B CN104007056B (en) | 2018-01-02 |
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| CN201410008695.8A Active CN104007056B (en) | 2013-02-22 | 2014-01-08 | To the evaluation method of the repellence of ultraviolet injury |
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| Country | Link |
|---|---|
| JP (1) | JP5775538B2 (en) |
| CN (1) | CN104007056B (en) |
| HK (1) | HK1198451A1 (en) |
| TW (1) | TWI612307B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109833479A (en) * | 2017-11-27 | 2019-06-04 | 上海交通大学 | It is a kind of to treat or prevent the drug and its application that Keratin 1 is degraded |
| CN109839507A (en) * | 2017-11-27 | 2019-06-04 | 江苏坤辉生物科技有限公司 | A kind of biomarker detecting ultraviolet light injury and its application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP6419654B2 (en) * | 2015-06-22 | 2018-11-07 | 株式会社ファンケル | Evaluation of hair growth and scalp compressive stress |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194022A (en) * | 2005-05-20 | 2008-06-04 | 普雷姆德股份有限公司 | Direct assay of skin protein in skin samples removed by tape stripping |
| CN101282708A (en) * | 2005-10-05 | 2008-10-08 | 江崎格力高株式会社 | External preparation for skin containing a phosphorlated saccharide |
| WO2011118812A1 (en) * | 2010-03-26 | 2011-09-29 | 国立大学法人北海道大学 | Neurodegenerative disease therapeutic agent |
| US20120114628A1 (en) * | 2008-11-21 | 2012-05-10 | L'oreal | Cosmetic and therapeutic use of proteins of dj-1 type for treating skin dryness |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009040753A (en) * | 2007-08-10 | 2009-02-26 | Advangen Inc | Skin repair composition and screening method thereof |
| US8212001B2 (en) * | 2009-09-17 | 2012-07-03 | Ramot At Tel-Aviv University Ltd. | Peptides for the treatment of oxidative stress related disorders |
-
2013
- 2013-02-22 JP JP2013032745A patent/JP5775538B2/en active Active
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2014
- 2014-01-08 CN CN201410008695.8A patent/CN104007056B/en active Active
- 2014-02-17 TW TW103105065A patent/TWI612307B/en active
- 2014-11-25 HK HK14111911A patent/HK1198451A1/en unknown
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101194022A (en) * | 2005-05-20 | 2008-06-04 | 普雷姆德股份有限公司 | Direct assay of skin protein in skin samples removed by tape stripping |
| CN101282708A (en) * | 2005-10-05 | 2008-10-08 | 江崎格力高株式会社 | External preparation for skin containing a phosphorlated saccharide |
| US20120114628A1 (en) * | 2008-11-21 | 2012-05-10 | L'oreal | Cosmetic and therapeutic use of proteins of dj-1 type for treating skin dryness |
| WO2011118812A1 (en) * | 2010-03-26 | 2011-09-29 | 国立大学法人北海道大学 | Neurodegenerative disease therapeutic agent |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109833479A (en) * | 2017-11-27 | 2019-06-04 | 上海交通大学 | It is a kind of to treat or prevent the drug and its application that Keratin 1 is degraded |
| CN109839507A (en) * | 2017-11-27 | 2019-06-04 | 江苏坤辉生物科技有限公司 | A kind of biomarker detecting ultraviolet light injury and its application |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5775538B2 (en) | 2015-09-09 |
| HK1198451A1 (en) | 2015-04-24 |
| CN104007056B (en) | 2018-01-02 |
| TWI612307B (en) | 2018-01-21 |
| JP2014163715A (en) | 2014-09-08 |
| TW201439538A (en) | 2014-10-16 |
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