CN104004615B - The application of endotoxin adsorbent in vinous fermentation - Google Patents
The application of endotoxin adsorbent in vinous fermentation Download PDFInfo
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- CN104004615B CN104004615B CN201310055641.2A CN201310055641A CN104004615B CN 104004615 B CN104004615 B CN 104004615B CN 201310055641 A CN201310055641 A CN 201310055641A CN 104004615 B CN104004615 B CN 104004615B
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Abstract
The invention discloses the application of a kind of endotoxin adsorbent in vinous fermentation.To be that endotoxin adsorbent prepared by raw material is applied to and waits to ferment or ferment in abnormal grape wine with yeast, due to the odd chain fatty acid in the adsorbable grape wine of endotoxin adsorbent and residues of pesticides, effective prevention or solve that the yeast that grape wine metabolic toxicities causes is poisoning, fermentation slowly or stagnate the problem of even fermenting and stopping, ensure that fermentation is carried out smoothly, avoid production accident, reach the object that safety is produced smoothly.The endotoxin adsorbent added, simultaneously for grape wine provides abundant Survival Factor, stimulates alcoholic fermentation and malolactic fermentation, is conducive to restarting or secondary fermentation, improves exterior quality vinous and structure sense, improve stability vinous.
Description
Technical field
The present invention relates to yeast extract technical field, in particular to the application of a kind of endotoxin adsorbent in vinous fermentation.
Background technology
Grape wine is one of large brewed wine (beer, grape wine, yellow rice wine) in the world three, the regulation of OIV is organized according to international grape wine, grape wine can only be broken or the alcoholic drink that obtains after yeast alcoholic fermentation wholly or in part of not broken fresh grape fruit or juice, and its alcoholic strength is generally between 8.5 ° ~ 16.2 °.The grape wine standard GB15037-2006 up-to-date according to China specifies, grape wine is that form through all or part of fermentation brew, alcoholic strength is not less than the alcoholic beverages of 7.0% with fresh grape or grape juice for raw material.
Pasteur once had ana: do not have grape wine without yeast! Refer to that the sugar in grape or grape juice is converted into alcohol, carbon dioxide and other accessory substances wholly or in part through the alcoholic fermentation of yeast, therefore yeast alcoholic fermentation is essential in Production of Wine.Under normal circumstances, alcoholic fermentation is except generating alcohol, fragrance and beautiful aroma substance, simultaneously also with the generation of some yeast metabolism toxin or existence mortifier, as odd chain fatty acid (sad, capric acid), metabolic toxicities and ochratoxin, or even some is attached to the residues of pesticides on Grape Skin surface, has negative effect equally.The existence of these materials can cause yeast poisoning, causes and ferments slowly or stagnate, termination of even fermenting, and then affect production safety, and as grape wine volatilizees, acid number raises, liquor output rate is low or residual sugar does not meet technological requirement or professional standard, causes production accident.Therefore during modern wine industry is produced, how to avoid occurring sluggish fermentation or stagnation or stopping, even how removing the fermentation toxin in vinous fermentation wine with dregs, residues of pesticides have necessary.
The solution of current routine is added mainly based on the nutritional agents of the inorganic salts of sulphur and phosphorus during the fermentation, maybe after appearance fermentation is abnormal, inoculation yeast add nutritional agents and restart fermentation again, needs to cultivate step by step, slowly amplify, needs higher professional operation skill, process is more loaded down with trivial details, be difficult to, control, sometimes even can not restart successfully, winemaker directly will make low-grade sweet wine or blended liquor, reduce value of the product, cause the loss of economic benefit.
Summary of the invention
The present invention aims to provide the application of a kind of endotoxin adsorbent in vinous fermentation, to solve the technical problem that the culture propagation existed in prior art is slow and stagnate.
To achieve these goals, according to an aspect of the present invention, provide the application of a kind of endotoxin adsorbent in vinous fermentation, endotoxin adsorbent is added to and waits to ferment or ferment in abnormal grape wine.
Further, the addition of endotoxin adsorbent accounts for and waits 0.02% ~ 0.04% of abnormal grape wine gross weight of fermenting or ferment.
Further, endotoxin adsorbent comprises following component: total nitrogen content counts 1.5% ~ 8.0% with butt, polysaccharide always adds up to 15 ~ 65% with glucan and mannose, fat content is 7 ~ 57%, content of mineral substances is 1.5 ~ 7.0%, moisture≤6.0%, ash content≤8.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, the pH value of the aqueous solution is 2.5 ~ 5.0.
Further, endotoxin adsorbent comprises following component: total nitrogen content counts 3.0 ~ 6.8% with butt, polysaccharide always adds up to 30 ~ 60% with glucan and mannose, fat content is 25 ~ 50%, content of mineral substances is 3.0 ~ 5.0%, moisture≤4.0%, ash content≤6.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, the pH value of the aqueous solution is 3.5 ~ 4.5.
Further, endotoxin adsorbent comprises following component: total nitrogen content counts 5.6% with butt, polysaccharide always adds up to 45% with glucan and mannose, fat content is 38.4%, content of mineral substances is 4.0%, and moisture is 3.0%, and ash content is 4.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, the pH value of the aqueous solution is 4.0.
Further, the preparation method of endotoxin adsorbent comprises: S1, to take saccharomyces cerevisiae as raw material preparation dry substance concentration be 10% ~ 18% yeast cell wall suspension; S2, yeast cell wall suspension is warming up to 50 DEG C ~ 80 DEG C, adjusts pH to 3.5 ~ 6.0, add acidity or alkali protease, obtain enzymolysis liquid; S3, go out to enzymolysis liquid ferment treatment, and centrifugation, obtains supernatant, carries out Vacuum Concentration to supernatant; And S4, the supernatant after Vacuum Concentration is carried out UF membrane, collect permeate, spraying dry, obtains endotoxin adsorbent.
Further, the acid or weight content of alkali protease in yeast cell wall suspension is 0.1% ~ 4.5%.
Further, the step of the ferment treatment that goes out comprises enzymolysis liquid is heated to 85 DEG C ~ 100 DEG C, continues 30 ~ 90 minutes.
Further, step with centrifugal separation is carried out under centrifugal force is the condition of 3500g ~ 4500g, and adopting in step S4 and shearing molecular weight is that the film of 400KD carries out UF membrane.
Further, in step S3, supernatant to be carried out supernatant Vacuum Concentration in the step of Vacuum Concentration to 10% ~ 40% of original volume.
The present invention will be that endotoxin adsorbent prepared by raw material is applied to and waits to ferment or ferment in abnormal grape wine with yeast, due to the odd chain fatty acid in the adsorbable grape wine of endotoxin adsorbent and residues of pesticides, effective prevention or solve that the yeast that grape wine metabolic toxicities causes is poisoning, fermentation slowly or stagnate the problem of even fermenting and stopping, ensure that fermentation is carried out smoothly, avoid production accident, reach the object that safety is produced smoothly.The endotoxin adsorbent added, simultaneously for grape wine provides abundant Survival Factor, stimulates alcoholic fermentation and malolactic fermentation, is conducive to restarting or secondary fermentation, improves exterior quality vinous and structure sense, improve stability vinous.
Accompanying drawing explanation
The Figure of description forming a application's part is used to provide a further understanding of the present invention, and schematic description and description of the present invention, for explaining the present invention, does not form inappropriate limitation of the present invention.In the accompanying drawings:
Fig. 1 shows according to the endotoxin adsorbent of exemplary embodiments of the present invention the schematic diagram of the residual treatment effect of grape juice agriculture.
Detailed description of the invention
It should be noted that, when not conflicting, the embodiment in the application and the feature in embodiment can combine mutually.Below with reference to the accompanying drawings and describe the present invention in detail in conjunction with the embodiments.
Endotoxin adsorbent in the present invention refers to yeast to be that raw material extracts and is prepared from, and it can adsorb odd chain fatty acid in the grape wine of fermentation and residues of pesticides, prevents sluggish fermentation or stagnation.
The invention provides the application of a kind of endotoxin adsorbent in vinous fermentation, by will be that endotoxin adsorbent prepared by raw material is applied to and waits to ferment or ferment in abnormal grape wine with yeast, odd chain fatty acid in endotoxin adsorbent absorption grape wine and residues of pesticides, effective prevention or solve that the yeast that grape wine metabolic toxicities causes is poisoning, fermentation slowly or stagnate the problem of even fermenting and stopping, ensure that fermentation is carried out smoothly, avoid production accident, reach the object that safety is produced smoothly.The endotoxin adsorbent added, simultaneously for grape wine provides abundant Survival Factor, stimulates alcoholic fermentation and malolactic fermentation, is conducive to restarting or secondary fermentation, improves exterior quality vinous and structure sense, improve stability vinous.
Vinous fermentation preparation process is roughly as follows: first grape stem removing fragmentation is obtained grape mash, grape mash is fermented, in grape mash (grape wine) to be fermented or started to ferment and occurred adding endotoxin adsorbent in abnormal grape wine, stir, continue fermentation dipping, slagging-off, filter, ageing, obtains grape wine.Occur slowly during the fermentation, stagnate, residual sugar more high abnormal problem time, mostly need to carry out inoculating yeast in prior art and restart fermentation.Creatively before fermentation or when fermentation starts or when fermentation occurs abnormal, endotoxin adsorbent is added in the present invention, the metabolic toxicities such as the endotoxin adsorbent added has supplementary sterol, Survival Factor effect, and also adsorbable LCFA, agriculture be residual stop preventing fermentation.Wherein endotoxin adsorbent can be waited to ferment or ferment in abnormal grape wine directly joining before inoculation yeast or together with yeast, stirs and makes it mix, can restart or secondary fermentation, and then avoids fermentation and stop phenomenon.
Except adding endotoxin adsorbent before fermentation or when fermentation starts, find exception of fermenting during the fermentation or close to during fermentation ends, toxin such as owing to producing in grape wine metabolic process causes yeast poisoning, make fermenting speed slow, endotoxin adsorbent can be added if there is during above-mentioned anomaly, the endotoxin adsorbent added due to containing manna oligosacchride composition, the odd chain fatty acid in the grape wine of adsorbable fermentation exception and residues of pesticides, and then improve problem slowly of fermenting.
In order to adsorb the metabolic toxicities produced in Fermentation of Grape Wine fully, supplement yeast Survival Factor, endotoxin adsorbent is played one's part to the full, and preferably, the addition of endotoxin adsorbent accounts for waits 0.02% ~ 0.04% of abnormal grape wine gross weight of fermenting or ferment.If the addition of endotoxin adsorbent is lower than 0.02%, toxin adsorption effect is not obvious, and toxin absorption not exclusively; If the addition of endotoxin adsorbent is higher than 0.04%, then can produce larger peculiar smell, and then affect the smell of product.
Endotoxin adsorbent in the present invention can adopt existing conventional method to be prepared from, the invention provides a kind of preparation method of preferred endotoxin adsorbent, the method comprises: S1, take saccharomyces cerevisiae as raw material preparation dry substance concentration be the yeast cream of 10 ~ 18%; S2, yeast cream is warming up to 50 DEG C ~ 80 DEG C, insulation 5 ~ 30min, adjust pH to 3.5 ~ 6.0, add acidity or alkali protease, isothermal holding 11 ~ 25h, obtains enzymolysis liquid; S3, go out to enzymolysis liquid ferment treatment, and centrifugation, obtains supernatant, carries out Vacuum Concentration to supernatant; And S4, the supernatant after Vacuum Concentration is carried out UF membrane, collect permeate, spraying dry, obtains endotoxin adsorbent.This preparation method's technique is simple, easy to operate.
Raw material of the present invention is saccharomyces cerevisiae (Saccharomycescerevisiae), can be other kind yeast cell wall in addition, and this raw material can be bought from market or obtain by being separated saccharomyces cerevisiae or other kind yeast autolysis.
A preferred embodiment of the invention, the acid or weight content of alkali protease in yeast cell wall suspension is 0.1% ~ 4.5%.Ferment treatment step of wherein going out comprises enzymolysis liquid is heated to 85 DEG C ~ 100 DEG C, continues 30 ~ 90 minutes.
According to a kind of exemplary embodiment of the present invention, step with centrifugal separation is carried out under centrifugal force is the condition of 3500g ~ 4500g, removes precipitation and obtain supernatant after centrifugation, carries out Vacuum Concentration make supernatant concentration to 10% ~ 40% of original volume to supernatant.Wherein adopting in step S4 and shearing molecular weight is that the film of 400KD carries out UF membrane, unwanted component can be filtered out, and then obtain the endotoxin adsorbent with required component.
Endotoxin adsorbent of the present invention is a kind of natural, safe, nontoxic bio-extract, is rich in the nutriment of tens kinds of needed by human body.The endotoxin adsorbent adopting said method to prepare comprises following component: total nitrogen content counts 1.5 ~ 8.0% with butt, polysaccharide always adds up to 15 ~ 65% with glucan and mannose, fat content is 7 ~ 57%, content of mineral substances is 1.5 ~ 7.0%, moisture≤6.0%, ash content≤8.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, the pH value of the aqueous solution is 2.5 ~ 5.0.
Although the endotoxin adsorbent of each component outside above-mentioned scope also can solve technical problem of the present invention, effect is effective not as the absorbing toxin of the endotoxin adsorbent of component in above-mentioned scope.Each component of endotoxin adsorbent is controlled the stability that can ensure product in above-mentioned scope further by the present invention.Also containing weight content in endotoxin adsorbent provided by the present invention is the fat of 7 ~ 57%, owing to containing the yeast existence materials such as sterol, sterol and phosphatide in lipoid material, can stimulate yeast activity, improves fermentation efficiency.Except containing except fat, the mineral matter also containing weight content 1.5% ~ 7.0%, mineral matter can make up the part of enzyme in yeast propagation metabolism, is also that yeast growth is necessary.
Preferably, total nitrogen content counts 3.0 ~ 6.8% with butt, polysaccharide always adds up to 30 ~ 60% with glucan and mannose, fat content is 25 ~ 50%, content of mineral substances is 3.0 ~ 5.0%, moisture≤4.0%, ash content≤6.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, the pH value of the aqueous solution is 3.5 ~ 4.5.Further preferably, endotoxin adsorbent comprises following component: total nitrogen content counts 5.6% with butt, polysaccharide always adds up to 45% with glucan and mannose, fat content is 38.4%, content of mineral substances is 4.0%, and moisture is 3.0%, and ash content is 4.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, the pH value of the aqueous solution is 4.0.
Endotoxin adsorbent preferably controls in above-mentioned scope by the present invention, although the endotoxin adsorbent of each constituent content outside above-mentioned scope also can connect solve technical problem of the present invention, not as effective when improving vinous fermentation of the endotoxin adsorbent of content in above-mentioned scope.
Beneficial effect of the present invention is further illustrated below in conjunction with specific embodiment.
Embodiment 1
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 10%, yeast cell wall suspension is warming up to 50 DEG C, be incubated 5 minutes, adjusted to ph is 3.5, adding alkali protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of alkali protease in yeast cell wall suspension be 0.1%, isothermal holding 11 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 85 DEG C continue to carry out going out ferment treatment in 30 minutes, carry out afterwards being continuously separated process under centrifugal force 3500g, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 10% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 400KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 1.5% with butt, polysaccharide always adds up to 65% with glucan and mannose, fat content is 18%, content of mineral substances is 1.5%, moisture is 6%, ash content is 8.0%, and pH value endotoxin adsorbent being formulated as the aqueous solution during aqueous solution of 2% is 2.5.
Embodiment 2
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 18%, yeast cell wall suspension is warming up to 80 DEG C, be incubated 30 minutes, adjusted to ph is 6.0, adding acid protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of acid protease in yeast cell wall suspension be 4.5%, isothermal holding 25 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 100 DEG C continue to carry out going out ferment treatment in 90 minutes, continuous flow centrifugation separating treatment is carried out afterwards under centrifugal force 4500g, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 40% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 400KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 8% with butt, polysaccharide always adds up to 15% with glucan and mannose, fat content is 57%, content of mineral substances is 7.0%, moisture is 5%, ash content is 8.0%, and pH value endotoxin adsorbent being formulated as the aqueous solution during aqueous solution of 2% is 4.0.
Embodiment 3
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 14%, yeast cell wall suspension is warming up to 65 DEG C, be incubated 18 minutes, adjusted to ph is 4.8, adding acid protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of acid protease in yeast cell wall suspension be 2.2%, isothermal holding 18 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 92 DEG C continue to carry out going out ferment treatment in 60 minutes, continuous flow centrifugation separating treatment is carried out afterwards under centrifugal force 4000g, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 25% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 400KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 3.0% with butt, polysaccharide always adds up to 60% with glucan and mannose, fat content is 25%, content of mineral substances is 3.0%, moisture is 5%, ash content is 4.0%, and pH value endotoxin adsorbent being formulated as the aqueous solution during aqueous solution of 2% is 4.0.
Embodiment 4
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 18%, yeast cell wall suspension is warming up to 80 DEG C, be incubated 30 minutes, adjusted to ph is 6.0, adding acid protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of acid protease in yeast cell wall suspension be 4.5%, isothermal holding 25 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 100 DEG C continue to carry out going out ferment treatment in 90 minutes, under centrifugal force 4500g, carry out continuous flow centrifuge afterwards carry out separating treatment, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 40% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 400KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 6.8% with butt, polysaccharide always adds up to 30% with glucan and mannose, fat content is 50%, content of mineral substances is 5.0%, moisture is 3%, ash content is 5.2%, and pH value endotoxin adsorbent being formulated as the aqueous solution during aqueous solution of 2% is 4.5.
Embodiment 5
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 15%, yeast cell wall suspension is warming up to 70 DEG C, be incubated 25 minutes, adjusted to ph is 5.8, adding alkali protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of alkali protease in yeast cell wall suspension be 3.8%, isothermal holding 20 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 92 DEG C continue to carry out going out ferment treatment in 60 minutes, continuous flow centrifugation separating treatment is carried out afterwards under centrifugal force 3800g, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 35% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 400KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 5.6% with butt, polysaccharide always adds up to 45% with glucan and mannose, fat content is 38.4%, content of mineral substances is 4.0%, moisture is 3.0%, ash content is 4.0%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, and the pH value of the aqueous solution is 4.0.
Embodiment 6
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 8%, yeast cell wall suspension is warming up to 45 DEG C, be incubated 6 minutes, adjusted to ph is 3.0, adding acid protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of acid protease in yeast cell wall suspension be 0.05%, isothermal holding 18 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 80 DEG C continue to carry out going out ferment treatment in 60 minutes, continuous flow centrifugation separating treatment is carried out afterwards under centrifugal force 2500g, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 55% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 400KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 1.0% with butt, polysaccharide always adds up to 70% with glucan and mannose, fat content is 2%, content of mineral substances is 10%, moisture is 7.0%, ash content is 10%, when endotoxin adsorbent being formulated as the aqueous solution of 2%, and the pH value of the aqueous solution is 6.0.
Embodiment 7
The saccharomyces cerevisiae (Angel Yeast Co., Ltd's production) that market is bought is mixed with the yeast cell wall suspension that dry substance concentration is 12%, yeast cell wall suspension is warming up to 60 DEG C, be incubated 10 minutes, adjusted to ph is 4.5, adding alkali protease (Pangbo Bioengineering Co Ltd, Nanning's production) makes the weight content of alkali protease in yeast cell wall suspension be 3.6%, isothermal holding 11 hours, obtains enzymolysis liquid.Enzymolysis liquid is heated to 85 DEG C continue to carry out going out ferment treatment in 30 minutes, continuous flow centrifugation separating treatment is carried out afterwards under centrifugal force 3500g, collect supernatant and carry out Vacuum Concentration, supernatant concentration is to 26% of original volume, the molecular weight of employing shearing is afterwards that the biomembrane of 5000KD carries out UF membrane, obtains permeate.Permeate spraying dry is obtained endotoxin adsorbent.Each constituent content wherein in endotoxin adsorbent is as follows: total nitrogen content counts 8% with butt, polysaccharide always adds up to 55% with glucan and mannose, fat content is 4%, content of mineral substances is 19%, moisture is 6%, ash content is 8.0%, and pH value endotoxin adsorbent being formulated as the aqueous solution during aqueous solution of 2% is 2.5.
Comparative example 1
The endotoxin adsorbent prepared in Example 1 ~ 7 respectively adds in the abnormal grape wine of fermentation, and initial pol when wherein adding initial in grape wine is 260g/L, and initial total yeast number is 10
6/ ml, fermentation temperature is 20 DEG C, continues fermentation after adding endotoxin adsorbent, and the phase detects yeast and fermented quality after fermentation, the results are shown in Table 1.
Table 1
As can be seen from Table 1, compared with comparative example 1, higher owing to all have employed endotoxin adsorbent the being fermented property pol in abnormal grape wine that makes to ferment in embodiment 1 ~ 7, the total yeast count in the grape wine of fermentation and active yeast number more.When the addition of endotoxin adsorbent is in 0.02% ~ 0.04% scope, adopt the adsorption effect of the endotoxin adsorbent of 0.04% addition better than the adsorption effect of the endotoxin adsorbent of employing 0.02% addition, and it is higher to be fermented pol, total yeast count and the viable yeast bacterium number in fermentation later stage are also higher, illustrate within the scope of the interpolation of 0.02% ~ 0.04%, along with the addition of endotoxin adsorbent increases, its ability of restarting fermentation strengthens.
When in embodiment 8, the addition of endotoxin adsorbent is 0.01%, compared with when being 0.02% ~ 0.04% with the addition of endotoxin adsorbent, it restarts fermentability reduction, the quantity being fermented sugar reduces greatly, the quantity of total yeast count of detecting of phase and viable yeast bacterium number is also lower after fermentation, and is also obviously deteriorated to the sad adsorption rate with capric acid.
Visible, by adding the endotoxin adsorbent of 0.02% ~ 0.04% in the grape wine to fermentation exception, yeasting can be improved, the metabolic toxicities produced in remarkable absorption sweat sad and certain herbaceous plants with big flowers acid etc., and along with increasing of endotoxin adsorbent addition, adsorption effect that is sad to metabolic toxicities and capric acid also increases.By removing the toxin produced in culture propagation, effective prevention or solve that the yeast that grape wine metabolic toxicities causes is poisoning, fermentation slowly or stagnate the problem of even fermenting and stopping, ensure that fermentation is carried out smoothly, promote yeast cells quality, promote alcoholic fermentation, and then improve grape wine mouthfeel and fragrance.
In order to further illustrate the adsorption effect of endotoxin adsorbent contratoxin of the present invention, the endotoxin adsorbent of preparation in embodiment 5 is added in grape juice, to detect its treatment effect to residues of pesticides, the testing result of testing result with the blank group of not adding endotoxin adsorbent compared, result as shown in Figure 1.As can be seen from Figure 1, after under equal conditions adding endotoxin adsorbent of the present invention in grape juice, compared with blank group that does not add endotoxin adsorbent, the agriculture residual in grape juice is as lower in fenisobromolate, procymidone, folpet and metalaxyl have by a relatively large margin.In often liter of agriculture raffinate, the fenisobromolate in blank group is about 1700 μ g, and after adding endotoxin adsorbent, fenisobromolate is about 1200 μ g, can reduce by 30%.Wherein the reduction amplitude of folpet is maximum, can reach 80%.
The effect in Fermentation of Grape Wine is being improved, below to stopping the grape wine fermenting to test in order to further illustrate endotoxin adsorbent of the present invention.Added in the grape wine stopping fermenting by the endotoxin adsorbent of preparation in embodiment 5 and restart fermentation to it, fermented wine degree when stopping fermentation is 10.5%, and fermentation temperature is 25 DEG C.Itself and the grape wine not adding endotoxin adsorbent are contrasted, test data is in table 2.
Table 2
As can be seen from Table 2, the blank group of not adding endotoxin adsorbent is then difficult to restart fermentation, even if it is also comparatively slow to start fermentation after fermentation, hypoglycemic is not thorough; And fermentation can be restarted to stopping the grape wine fermenting after with the addition of endotoxin adsorbent of the present invention, and restart fermentation comparatively fast, the phase after fermentation, significantly can strengthen pol utilization rate, reduce the residual sugar amount in fermented wine, hypoglycemic is thorough.Visible, the endotoxin adsorbent of interpolation, simultaneously for grape wine provides abundant Survival Factor, stimulates alcoholic fermentation and malolactic fermentation, is conducive to restarting or secondary fermentation, improves exterior quality vinous and structure sense, improve stability vinous.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (7)
1. the application of endotoxin adsorbent in vinous fermentation, is characterized in that, is added to by described endotoxin adsorbent and waits to ferment or ferment in abnormal grape wine;
Described endotoxin adsorbent comprises following component: total nitrogen content counts 1.5% ~ 8.0% with butt, polysaccharide always adds up to 15 ~ 65% with glucan and mannose, fat content is 7 ~ 57%, content of mineral substances is 1.5 ~ 7.0%, moisture≤6.0%, ash content≤8.0%, when described endotoxin adsorbent is formulated as the aqueous solution of 2%, the pH value of the described aqueous solution is 2.5 ~ 5.0;
The preparation method of described endotoxin adsorbent comprises:
S1, to take saccharomyces cerevisiae as raw material preparation dry substance concentration be 10% ~ 18% yeast cell wall suspension;
S2, described yeast cell wall suspension is warming up to 50 DEG C ~ 80 DEG C, adjusts pH to 3.5 ~ 6.0, add acidity or alkali protease carries out enzymolysis, obtain enzymolysis liquid;
S3, go out to described enzymolysis liquid ferment treatment, and centrifugation, obtains supernatant, carries out Vacuum Concentration to described supernatant; And
S4, the supernatant after Vacuum Concentration is carried out UF membrane, collect permeate, spraying dry, obtains described endotoxin adsorbent;
Described step with centrifugal separation is carried out under centrifugal force is the condition of 3500g ~ 4500g, and adopting in described step S4 and shearing molecular weight is that the film of 400KD carries out UF membrane.
2. the application of endotoxin adsorbent according to claim 1 in vinous fermentation, is characterized in that, waits 0.02% ~ 0.04% of abnormal grape wine gross weight of fermenting or ferment described in the addition of described endotoxin adsorbent accounts for.
3. the application of endotoxin adsorbent according to claim 1 in vinous fermentation, it is characterized in that, described endotoxin adsorbent comprises following component: total nitrogen content counts 3.0 ~ 6.8% with butt, polysaccharide always adds up to 30 ~ 60% with glucan and mannose, and fat content is 25 ~ 50%, and content of mineral substances is 3.0 ~ 5.0%, moisture≤4.0%, ash content≤6.0%, when described endotoxin adsorbent is formulated as the aqueous solution of 2%, the pH value of the described aqueous solution is 3.5 ~ 4.5.
4. the application of endotoxin adsorbent according to claim 1 in vinous fermentation, it is characterized in that, described endotoxin adsorbent comprises following component: total nitrogen content counts 5.6% with butt, polysaccharide always adds up to 45% with glucan and mannose, and fat content is 38.4%, and content of mineral substances is 4.0%, moisture is 3.0%, ash content is 4.0%, and when described endotoxin adsorbent is formulated as the aqueous solution of 2%, the pH value of the described aqueous solution is 4.0.
5. the application of endotoxin adsorbent according to claim 1 in vinous fermentation, is characterized in that, described acidity or the weight content of alkali protease in described yeast cell wall suspension are 0.1% ~ 4.5%.
6. the application of endotoxin adsorbent according to claim 1 in vinous fermentation, is characterized in that, described in the go out step of ferment treatment comprise described enzymolysis liquid be heated to 85 DEG C ~ 100 DEG C, continue 30 ~ 90 minutes.
7. the application of endotoxin adsorbent according to claim 1 in vinous fermentation, is characterized in that, the step of carrying out Vacuum Concentration to described supernatant in described step S3 is to 10% ~ 40% of original volume by described supernatant Vacuum Concentration.
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| CN102424795A (en) * | 2011-12-21 | 2012-04-25 | 安琪酵母股份有限公司 | Biological aging-accelerating additive for white wine |
| CN102548568A (en) * | 2009-01-14 | 2012-07-04 | 全技术公司 | Clay interlaced yeast compositions and methods of utilizing the same |
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| US8318475B2 (en) * | 2009-01-14 | 2012-11-27 | Alltech, Inc. | Clay interlaced yeast compositions and methods of utilizing the same |
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| CN102548568A (en) * | 2009-01-14 | 2012-07-04 | 全技术公司 | Clay interlaced yeast compositions and methods of utilizing the same |
| CN102051400A (en) * | 2009-11-06 | 2011-05-11 | 安琪酵母股份有限公司 | Preparation method of yeast mannose glycoprotein product |
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