CN103992489A - Novel green processing method for increasing resistant starch content through crosslinking of starch and chitosan - Google Patents
Novel green processing method for increasing resistant starch content through crosslinking of starch and chitosan Download PDFInfo
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- CN103992489A CN103992489A CN201410105038.5A CN201410105038A CN103992489A CN 103992489 A CN103992489 A CN 103992489A CN 201410105038 A CN201410105038 A CN 201410105038A CN 103992489 A CN103992489 A CN 103992489A
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Abstract
The invention relates to crosslinking of starch and chitosan in specific circumstances, and especially a processing technology for increasing resistant starch content through crosslinking of starch and chitosan. Chitosan and waxy maize starch are subjected to spray drying in an acetic acid solution system to prepare a blend; then the blend in the anhydrous limit state is subjected to thermal crosslinking for 2 h; and the blend sample is analyzed and determined, and molecular structure of the sample is studied through infrared spectrometer and nuclear magnetic resonance mass spectrometer. The results show obvious difference between the molecular structure of the blends and that of a control, it instructs that chitosan and starch conduct certain cross-linking reaction rather than a simple physical blending, through drying thermal treatment, to generate a novel structure. At the same time, a scanning electron microscope is used to observe the particle morphology of the blend sample before and after the gelatinization. The blend sample is subjected to a digestion test in a simulated in vivo digestive system test to obtain the resistant starch.
Description
Affiliated technical field
Patent of the present invention relate to starch and chitosan crosslinked, particularly waxy corn starch and chitosan crosslinked after can improve the complete processing of resistant starch content.The present invention is the research and development of a kind of new green processing method to raising resistant starch content, thereby processes the crosslinked resistant starch content that improves of starch and chitosan without chemical solvents method, is a kind of research of novel method.
Background technology
Resistant starch glucose in digestive process discharges slowly, this becomes the focus of a lot of Experts ' Attentions, its preparation method at home and abroad has much at present, mainly contain microwave treatment method, ultrasonication method, method of chemical treatment and hydrolysis and press hot method etc., and these methods also exists many deficiencies.For example, microwave treatment sample can be subject to the impact of Heating temperature and moisture, and various physico-chemical properties are had a certain impact, and ultrasonication can make starch degradation, but its degraded after molecular weight distribution narrower than other degradation method.
In addition, research shows that chitosan can not be well digested, but there are good biocidal property, biological degradability and film-forming properties, at present research focuses mostly in the preparation of starch and chitosan blend thing antibacterial film, have not been reported and pass through the research that special dry heat treatment prepares starch granules after the two mixes on molecular level.
Summary of the invention
Main purpose of the present invention is to provide a kind of resistant starch product, and use the green preparation of the method without chemical solvents, to eliminate the shortcoming in current institute employing method, this method is not added other chemical reagent, take full advantage of the advantage of chitosan self, prepared product had both had certain anti-microbial property, also can effectively improve the content of Resistant starch, slowed down its digestion rate.By this sample is carried out to test determination, find that this Resistant starch has higher weave construction, can be observed its surface with sweep electron microscope has one deck uniform thin film to cover, and this may be exactly the reason that slows down starch digestion.Feature of the present invention is exactly to utilize physical method can obtain the achievement of chemical process, and therefore present method is different from simply by the combination of physical method and chemical process, and it is simple, green non-pollution.
In order to realize above-mentioned object, the present invention adopts following technology incidence of criminal offenses: (1) adopts waxy corn starch and chitosan blend in acetic acid solution, taking the waxy corn starch that do not add chitosan as control sample, carries out spray dried dry after finishing dealing with; (2) spraying dried laboratory sample and control sample are dried under 45 DEG C of drying conditionss non-binding in starch and in conjunction with water, then heat cross-linking 2 hours under 135 DEG C of conditions; (3) utilize Fourier transform infrared spectrometer, mass spectrum of nuclear magnetic resonance instrument to analyze mensuration to sample, and then the composition structure of study sample, carry out the granule-morphology of observing samples simultaneously with sweep electron microscope; (4) starch 200mg different treatment being crossed carries out external digestion test in simulation human small intestine system, can obtain a certain amount of Resistant starch, analyzes relatively with control sample.
Brief description of the drawings:
Fig. 1 is the starch scanning electron microscope (SEM) photograph with specific morphology: have the uniform film of one deck and be overlying on particle surface and be more conducive to slow down digestion
Fig. 2 is starch scanning electron microscope (SEM) photograph after gelatinization: part exists the even small-particle of a lot of ungelatinizeds, can tentatively be inferred as starch and the interactional product of chitosan
Fig. 3 is the digestion characteristics of xeothermic rear different starch: result demonstration, with ative starch comparison, its digestion rate obviously reduces.
Embodiment:
Chitosan, waxy corn starch blend, through 1% acetic acid solution processing, carry out xeothermic crosslinked 2 hours under limit anhydrous state, not add the waxy corn starch of chitosan and ative starch as control sample.Starch can obtain Resistant starch after simulation human small intestine Digestive tract digests.And starch sample is analyzed to mensuration, by the molecular structure of infrared spectrometer, mass spectrum of nuclear magnetic resonance instrument study sample, result shows that blend molecular structure is obviously different from control sample, illustrate that chitosan and starch are not simple physical blending, but after dry heat treatment, there is certain crosslinking reaction, produce new structure.The observation of sample after gelatinization being carried out to scanning electronic microscope also has the small-particle that is much difficult to uniformly gelatinization to exist, and the generation that this has also just confirmed Resistant starch, has improved 7.5% than the content of Resistant starch in ative starch.
Claims (5)
- Thereby 1. a novel method that effectively improves resistant starch content by the green processes craft cross-linked chitosan without chemical solvents method and starch, its principal character is: the blend of (1) chitosan, starch; (2) dry heat treatment of blend; (3) Resistant starch is prepared in the digestion trial of blend under simulation human small intestine environment; (4) mensuration of blend sample particle molecular structure.
- 2. according to the desired novel method of right 1, it is characterized in that: adopt chitosan, waxy corn starch blend in 1% acetic acid solution system, spray afterwards dry, and carry out heat treated crosslinking reaction under pure anhydrous condition.
- 3. according to the desired novel method of right 1, it is characterized in that: the external digestion test that chitosan-starch blending matter sample 200mg is carried out at simulated in vivo environment, sample is mixed with salivin, and add pancreas enzyme and pig source stomach en-, and then add saccharifying enzyme and further degrade, carry out reaction after 12 hours and finish, its content detects with glucolase preparation box, can obtain a certain amount of Resistant starch.And carry out the calculating of digestion rate simultaneously, digestion rate has reduced by 13% than ative starch.
- 4. according to the desired novel method of right 1, it is characterized in that: utilize infrared spectrometer and mass spectrum of nuclear magnetic resonance instrument to analyze mensuration to sample, the molecular structure of research blend sample is observed the granule-morphology before and after the gelatinization of blend sample with sweep electron microscope simultaneously.Before gelatinization, sample particle surface has thin film to cover, and the sample after gelatinization has occurred that many small-particles of uniform size are difficult to gelatinization.
- 5. according to the desired novel method of right 1, it is characterized in that: use granule-morphology and the external digestion testing data of the sample gelatinization front and back of scanning electron microscopic observation to analyze relatively, institute's expression characteristics matches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410105038.5A CN103992489A (en) | 2014-03-14 | 2014-03-14 | Novel green processing method for increasing resistant starch content through crosslinking of starch and chitosan |
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| CN201410105038.5A CN103992489A (en) | 2014-03-14 | 2014-03-14 | Novel green processing method for increasing resistant starch content through crosslinking of starch and chitosan |
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| CN103992489A true CN103992489A (en) | 2014-08-20 |
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| CN201410105038.5A Pending CN103992489A (en) | 2014-03-14 | 2014-03-14 | Novel green processing method for increasing resistant starch content through crosslinking of starch and chitosan |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104225662A (en) * | 2014-09-28 | 2014-12-24 | 沈丽青 | Hemostatic product and preparation method thereof |
| CN104777025A (en) * | 2015-04-24 | 2015-07-15 | 甘肃农业大学 | Method for preparing scanning electron microscope sample of silage corn leaf |
| CN105254931A (en) * | 2015-11-20 | 2016-01-20 | 保龄宝生物股份有限公司 | Development of novel product for improving properties of crosslinked-acetylated starch particles by introducing chitosan |
| CN106380636A (en) * | 2016-08-30 | 2017-02-08 | 华南理工大学 | Chitosan-grafted resistant starch carrier material and its preparation method and use |
| CN106616926A (en) * | 2016-12-26 | 2017-05-10 | 大连民族大学 | Method for preparing slowly digestible corn starch by carrying out dry heat treatment |
| CN109022519A (en) * | 2018-08-29 | 2018-12-18 | 浙江工商大学 | The method of high-purity resistant starch is efficiently prepared under a kind of cryogenic conditions |
| CN109916844A (en) * | 2019-04-15 | 2019-06-21 | 长江大学 | Method for rapidly determining resistant starch content of wheat grains |
| CN110538344A (en) * | 2019-10-09 | 2019-12-06 | 北京诺康达医药科技股份有限公司 | Medical degradable hemostatic material and preparation method thereof |
| CN114409815A (en) * | 2022-03-30 | 2022-04-29 | 广东海天创新技术有限公司 | Preparation method of crosslinked starch |
-
2014
- 2014-03-14 CN CN201410105038.5A patent/CN103992489A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104225662A (en) * | 2014-09-28 | 2014-12-24 | 沈丽青 | Hemostatic product and preparation method thereof |
| CN104225662B (en) * | 2014-09-28 | 2016-03-23 | 沈丽青 | Hemostasia products and preparation method thereof |
| CN104777025A (en) * | 2015-04-24 | 2015-07-15 | 甘肃农业大学 | Method for preparing scanning electron microscope sample of silage corn leaf |
| CN105254931A (en) * | 2015-11-20 | 2016-01-20 | 保龄宝生物股份有限公司 | Development of novel product for improving properties of crosslinked-acetylated starch particles by introducing chitosan |
| CN106380636A (en) * | 2016-08-30 | 2017-02-08 | 华南理工大学 | Chitosan-grafted resistant starch carrier material and its preparation method and use |
| CN106616926A (en) * | 2016-12-26 | 2017-05-10 | 大连民族大学 | Method for preparing slowly digestible corn starch by carrying out dry heat treatment |
| CN109022519A (en) * | 2018-08-29 | 2018-12-18 | 浙江工商大学 | The method of high-purity resistant starch is efficiently prepared under a kind of cryogenic conditions |
| CN109022519B (en) * | 2018-08-29 | 2021-11-23 | 浙江工商大学 | Method for preparing high-purity resistant starch under low-temperature condition |
| CN109916844A (en) * | 2019-04-15 | 2019-06-21 | 长江大学 | Method for rapidly determining resistant starch content of wheat grains |
| CN110538344A (en) * | 2019-10-09 | 2019-12-06 | 北京诺康达医药科技股份有限公司 | Medical degradable hemostatic material and preparation method thereof |
| CN114409815A (en) * | 2022-03-30 | 2022-04-29 | 广东海天创新技术有限公司 | Preparation method of crosslinked starch |
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Application publication date: 20140820 |