CN103992414A - Extraction method for polysaccharide in corn straws - Google Patents
Extraction method for polysaccharide in corn straws Download PDFInfo
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- CN103992414A CN103992414A CN201410179146.7A CN201410179146A CN103992414A CN 103992414 A CN103992414 A CN 103992414A CN 201410179146 A CN201410179146 A CN 201410179146A CN 103992414 A CN103992414 A CN 103992414A
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Abstract
The invention discloses an extraction method for polysaccharide in corn straws. The extraction method comprises: drying and crushing, preparing a mixed solution, filtering and centrifuging, preparing a concentrated solution, removing protein by employing a Sevage method, purifying and centrifuging, redissolving precipitate, and preparing polysaccharide. The extraction method has the characteristics that the prepared polysaccharide is natural, purely fragrant and healthy, is applicable to foodstuff processing and helps to improve the quality of foodstuff; the extraction technology is simple in process, produced polysaccharide is high in purity and less in impurities, and large-scale production can be realized; and waste is changed into valuables, corn straw waste problem is solved, and the economic benefit is high.
Description
Technical field
The invention belongs to a kind of extracting method of polysaccharide, be specifically related to the extracting method of polysaccharide in a kind of maize straw.
Background technology
Polysaccharide has and strengthens immunity, radioprotective, antitumor, anti-inflammatory, the biologic activity widely such as hypoglycemic, is more and more subject to people's common concern.And corn is one of staple crops of China, in China, maize sown area and output Jun Ju second place of the world, the maize straw being produced by maize planting can reach hundred million tons.In maize straw, contain abundant saccharan, yet at present maize straw, mostly as the feed of livestock or directly lose, causes significant wastage.
At present, generally in traditional industry adopt that acid is carried, alkali is carried, this can destroy its molecular structure to a certain extent, thereby affects its biological activity, and in industrialization livestock, uses corrosion and the environmental pollution that will inevitably cause equipment after a large amount of soda acids.
Summary of the invention
The object of the present invention is to provide the extracting method of polysaccharide in a kind of maize straw, adopted hot water extraction, environment protection health, safe and reliable, and purity is high, and preparation flow is simple.
In a kind of maize straw of the present invention, the technical scheme of the extracting method of polysaccharide is achieved in that
An extracting method for polysaccharide in maize straw, by following steps, realize:
(1) foreign material of maize straw are removed, be then dried, making maize straw water ratio is 25～30%, then pulverizes, and crosses 60 mesh sieves;
(2) get maize straw powder in beaker, add the water of 30～40 times, in 80～100 ℃, extract 3～5 hours, make mixed solution;
(3) mixed solution is carried out to vacuum filtration, filtrate is separated with filter residue, and gained filter residue repeats (2) step, then filtrate is merged, then uses whizzer centrifugal;
(4) filtrate after centrifugal is put in 2～5 ℃ of environment and placed 5～8 hours, then filter, then put into Rotary Evaporators and concentrate with the rotating speed of 30～60rpm/s, solution to be concentrated occurs that wall cling phenomenon stops concentrating, and makes concentrated solution;
(5) Sevage method Deproteinization: extracting solution is mixed with 5:1 ratio with Sevage reagent, vibration, centrifugal;
(6) in centrifugate, add ethanol, make alcohol concn reach 80%, standing over night, then obtains throw out with the centrifugal 4～8min of 4000r/min;
(7) throw out is multiple water-soluble, centrifugal, then supernatant liquor is put into Freeze Drying Equipment freeze-drying, make polysaccharide.
For polysaccharide is dissolved in solution more, better must purification, in step (4), Rotary Evaporators rotating speed is 45～50rpm/s.
In order to make the purity of polysaccharide higher, impurity is few, step (5) Sevage reagent refers to that chloroform and propyl carbinol are with the mixed solution of 5:1 volume ratio.
Compared with prior art, the present invention has the following advantages: natural, the pure perfume (or spice) of polysaccharide, health that the present invention prepares, can be used for food-processing, and improve the quality of food; Extraction process flow process of the present invention is simple, and the purity of polysaccharide produced is high, impurity is few, can realize scale operation; The present invention is turned waste into wealth, and can solve maize straw waste problem, and can increase farmers' income.
Embodiment
Embodiment 1
An extracting method for polysaccharide in maize straw, by following steps, realize:
(1) foreign material of maize straw are removed, be then dried, making maize straw water ratio is 25%, then pulverizes, and crosses 60 mesh sieves;
(2) get maize straw powder in beaker, add the water of 40 times, in 100 ℃, extract 3 hours, make mixed solution;
(3) mixed solution is carried out to vacuum filtration, filtrate is separated with filter residue, and gained filter residue repeats (2) step, then filtrate is merged, then uses whizzer centrifugal;
(4) filtrate after centrifugal is put in 2 ℃ of environment and placed 8 hours, then filter, then put into Rotary Evaporators and concentrate with the rotating speed of 45rpm/s, solution to be concentrated occurs that wall cling phenomenon stops concentrating, and makes concentrated solution;
(5) Sevage method Deproteinization: extracting solution is mixed with 5:1 ratio with Sevage reagent, vibration, centrifugal; Wherein, Sevage reagent is the mixed solution that chloroform and propyl carbinol volume ratio are 5:1;
(6) in centrifugate, add ethanol, make alcohol concn reach 80%, standing over night, then obtains throw out with the centrifugal 4min of 4000r/min;
(7) throw out is multiple water-soluble, centrifugal, then supernatant liquor is put into Freeze Drying Equipment freeze-drying, make polysaccharide.
Embodiment 2
An extracting method for polysaccharide in maize straw, by following steps, realize:
(1) foreign material of maize straw are removed, be then dried, making maize straw water ratio is 30%, then pulverizes, and crosses 60 mesh sieves;
(2) get maize straw powder in beaker, add the water of 30 times, in 80 ℃, extract 5 hours, make mixed solution;
(3) mixed solution is carried out to vacuum filtration, filtrate is separated with filter residue, and gained filter residue repeats (2) step, then filtrate is merged, then uses whizzer centrifugal;
(4) filtrate after centrifugal is put in 5 ℃ of environment and placed 5 hours, then filter, then put into Rotary Evaporators and concentrate with the rotating speed of 45～50rpm/s, solution to be concentrated occurs that wall cling phenomenon stops concentrating, and makes concentrated solution;
(5) Sevage method Deproteinization: extracting solution is mixed with 5:1 ratio with Sevage reagent, vibration, centrifugal; Wherein, Sevage reagent is the mixed solution that chloroform and propyl carbinol volume ratio are 5:1;
(6) in centrifugate, add ethanol, make alcohol concn reach 80%, standing over night, then obtains throw out with the centrifugal 8min of 4000r/min;
(7) throw out is multiple water-soluble, centrifugal, then supernatant liquor is put into Freeze Drying Equipment freeze-drying, make polysaccharide.
Embodiment 3
An extracting method for polysaccharide in maize straw, by following steps, realize:
(1) foreign material of maize straw are removed, be then dried, making maize straw water ratio is 28%, then pulverizes, and crosses 60 mesh sieves;
(2) get maize straw powder in beaker, add the water of 35 times, in 90 ℃, extract 4 hours, make mixed solution;
(3) mixed solution is carried out to vacuum filtration, filtrate is separated with filter residue, and gained filter residue repeats (2) step, then filtrate is merged, then uses whizzer centrifugal;
(4) filtrate after centrifugal is put in 3 ℃ of environment and placed 6 hours, then filter, then put into Rotary Evaporators and concentrate with the rotating speed of 48rpm/s, solution to be concentrated occurs that wall cling phenomenon stops concentrating, and makes concentrated solution;
(5) Sevage method Deproteinization: extracting solution is mixed with 5:1 ratio with Sevage reagent, vibration, centrifugal; Wherein, Sevage reagent is the mixed solution that chloroform and propyl carbinol volume ratio are 5:1;
(6) in centrifugate, add ethanol, make alcohol concn reach 80%, standing over night, then obtains throw out with the centrifugal 6min of 4000r/min;
(7) throw out is multiple water-soluble, centrifugal, then supernatant liquor is put into Freeze Drying Equipment freeze-drying, make polysaccharide.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. an extracting method for polysaccharide in maize straw, is characterized in that, comprises the following steps:
(1) foreign material of maize straw are removed, be then dried, making maize straw water ratio is 25～30%, then pulverizes, and crosses 60 mesh sieves;
(2) get maize straw powder in beaker, add the water of 30～40 times, in 80～100 ℃, extract 3～5 hours, make mixed solution;
(3) mixed solution is carried out to vacuum filtration, filtrate is separated with filter residue, and gained filter residue repeats (2) step, then filtrate is merged, then uses whizzer centrifugal;
(4) filtrate after centrifugal is put in 2～5 ℃ of environment and placed 5～8 hours, then filter, then put into Rotary Evaporators and concentrate with the rotating speed of 30～60rpm/s, solution to be concentrated occurs that wall cling phenomenon stops concentrating, and makes concentrated solution;
(5) Sevage method Deproteinization: extracting solution is mixed with 5:1 ratio with Sevage reagent, vibration, centrifugal;
(6) in centrifugate, add ethanol, make alcohol concn reach 80%, standing over night, then obtains throw out with the centrifugal 4～8min of 4000r/min;
(7) throw out is multiple water-soluble, centrifugal, then supernatant liquor is put into Freeze Drying Equipment freeze-drying, make polysaccharide.
2. the extracting method of polysaccharide in a kind of maize straw as claimed in claim 1, is characterized in that, in step (4), Rotary Evaporators rotating speed is 45～50rpm/s.
3. the extracting method of polysaccharide in a kind of maize straw as claimed in claim 1, is characterized in that, step (5) Sevage reagent refers to that chloroform and propyl carbinol are with the mixed solution of 5:1 volume ratio.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410179146.7A CN103992414A (en) | 2014-04-30 | 2014-04-30 | Extraction method for polysaccharide in corn straws |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410179146.7A CN103992414A (en) | 2014-04-30 | 2014-04-30 | Extraction method for polysaccharide in corn straws |
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| CN103992414A true CN103992414A (en) | 2014-08-20 |
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| CN201410179146.7A Pending CN103992414A (en) | 2014-04-30 | 2014-04-30 | Extraction method for polysaccharide in corn straws |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111978428A (en) * | 2020-09-08 | 2020-11-24 | 綦峥 | Separation and purification method and application of soybean straw polysaccharide for inhibiting human liver cancer cells |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100044627A1 (en) * | 2008-08-22 | 2010-02-25 | Robert Lee Barcus | Thermoplastic heteropolysaccharide derivatives and methods for making same |
| CN101691617A (en) * | 2009-09-11 | 2010-04-07 | 南京工业大学 | Method for preparing soluble sugar by degrading crop straws |
| CN103044568A (en) * | 2013-01-11 | 2013-04-17 | 河北工业大学 | Plant heteropolysaccharide sulfonate, as well as preparation method and application thereof |
-
2014
- 2014-04-30 CN CN201410179146.7A patent/CN103992414A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100044627A1 (en) * | 2008-08-22 | 2010-02-25 | Robert Lee Barcus | Thermoplastic heteropolysaccharide derivatives and methods for making same |
| CN101691617A (en) * | 2009-09-11 | 2010-04-07 | 南京工业大学 | Method for preparing soluble sugar by degrading crop straws |
| CN103044568A (en) * | 2013-01-11 | 2013-04-17 | 河北工业大学 | Plant heteropolysaccharide sulfonate, as well as preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 张培刚等: "玉米秸秆中多糖的提取及单糖组成研究", 《教育教学论坛》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111978428A (en) * | 2020-09-08 | 2020-11-24 | 綦峥 | Separation and purification method and application of soybean straw polysaccharide for inhibiting human liver cancer cells |
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Application publication date: 20140820 |