CN103983576B - Full-automatic chemiluminescence imaging device - Google Patents
Full-automatic chemiluminescence imaging device Download PDFInfo
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- CN103983576B CN103983576B CN201410231294.9A CN201410231294A CN103983576B CN 103983576 B CN103983576 B CN 103983576B CN 201410231294 A CN201410231294 A CN 201410231294A CN 103983576 B CN103983576 B CN 103983576B
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Abstract
The present invention relates to detection technique field, be specifically related to a kind of Full-automatic chemiluminescence imaging device. The present invention compared with prior art adopts integrated industrial design, effectively saves space. Full-automatic imaging control, one-key operation luminescence imaging operation: high-precision full-automatic exposure, a key is taken sample image, is automatically saved to USB flash disk, without setting time for exposure and camera lens parameters, simple to operate. Once take and can obtain multiple time for exposure effect image, and can be displayed in full screen or split screen shows multiple images simultaneously, conveniently observe contrast. Also possesses a key printing function, printable any design sketch.
Description
Technical field
The present invention relates to detection technique field, be specifically related to a kind of Full-automatic chemiluminescence imaging device.
Background technology
Along with the development of digitized video technology, make trace luminescence technology become the most frequently used means in the research such as protein and nucleic acid, therefore there is the more chemiluminescence imaging system of high sensitivity and data analysis function and also replace gradually traditional Film Dosimeter, become the instrument of each laboratory indispensability that relates to life science.
And the operation of conventional chemical luminescence imaging system is more loaded down with trivial details on market, consuming time longer, flow process is as follows: 1. open image capture software 2. and wait for and camera cooling 3. open respective sources 4. camera preview 5. adjustable lens parameters (focusing, aperture, distance) 6. the estimated exposure time 7. takes and checks that image effect 8. again takes and check image effect until the 10. preservation pictures that meet the requirements according to the effect amendment time for exposure 9..
Summary of the invention
The object of the invention is to the defect existing for prior art, a kind of new automatic chemiluminescence imaging device is provided, make user use more shirtsleeve operation to obtain better chemiluminescence image, also can copying image be done to further processing to the PC that professional image analysis software is housed to image by USB flash disk.
The present invention adopts following technical proposals:
A kind of Full-automatic chemiluminescence imaging device, comprise outer cabinet, camera bellows, power supply, image capture system, control and communicating circuit system, illuminator, objective table, storage system, display system and startup button, start by key control electric power starting, Power supply is to image capture system, control and communicating circuit system, storage system and illuminator, and control and communicating circuit system connect respectively image capture system, storage system, display system and illuminator; It is characterized in that sample is placed in objective table, close outer cabinet, by starting button, power supply energising, control and communicating circuit system initialization, detected image capture systems temperature, arranges shooting option, detection of stored system, illuminator is opened, automatic shooting, and the automatic exposure time is pressed following algorithm:
In formula: t0For the pre-exposure time, the gray scale array of its imaging is G, k=0.0333 (grey jump > 100) or k=0.0033 (grey jump≤100), m=Max (int (G × 10-2))，n＝Min(int(G×10-2))；
Take white light egative film, illuminator is closed, and then takes chemiluminescence picture, image stack or/or inverse processing, image single screen or split screen or Overlapping display, image storage, powered-down.
In certain embodiments, described Full-automatic chemiluminescence imaging device can comprise illuminator. Can use any applicable illuminator. In certain embodiments, described illuminator can comprise at least one light source. Described light source can transmitting white maybe can be launched the light of any applicable wavelength, for example, can be used for the light of the wavelength of exciting light sensitive molecule, and described photosensitive molecular can be positioned at sample and/or comprise the sample that is that the chemiluminescence box of described system is processed. In certain embodiments, in the time sample being placed on objective table or objective table to chemiluminescence box, at least one light source can be set in order to irradiate at least one sample. In certain embodiments, described at least one light source can comprise or be made up of single lamp source, many lamp source, one or more LED or one or more LED array. In certain embodiments, described at least one light source can transmitting white and can be comprised one or more wave filters, and described wave filter can filtering irradiates sample in described device and be placed in the light of the undesirable wavelength of chemiluminescence box on objective table or objective table. Undesirable wavelength that can filter can comprise that the mode of the clear and definite signal that is not suitable for being provided for image capture system identification excites the wavelength of relative photo sensitive molecule, maybe can comprise the wavelength overlapping with transmitted beam that will imaging.
For example, in certain embodiments, described light source can comprise blue-light source, as blue LED, blue lamp, or white LEDs or the lamp that uses together with blue wave filter. The wave-length coverage of the blue light of being launched by blue-light source from about 435nm to about 500nm, from about 440nm to about 490nm, for example, from about 450nm to about 475nm, from about 460nm to about 470nm or about 475nm. In this example, the excitation wavelength range that can be used for the photosensitive molecular of sample that mark just processing on objective table or standard items can be for from about 440nm to about 490nm, for example wavelength from about 450nm to about 475nm, from about 460nm to about 470nm or about 475nm.
In certain embodiments, the wave-length coverage of light source is in about 20nm, and 20nm is the maximum excitation wavelength of photosensitive molecular. Should be understood that and can use any applicable photosensitive molecular, and than the wave-length coverage of above-mentioned blue light of giving an example, these photosensitive moleculars can have different excitation wavelength range. In this case, can use the applicable lamp of suitable excitation wavelength, LED, wave filter and other opticses are provided.
In certain embodiments, described illuminator can comprise more than one light source. In certain embodiments, described irradiation system can have at least one light source for each sample/chemiluminescence case assembly on objective table in described device. In certain embodiments, described illuminator can comprise the more than one light source for each chemiluminescence case assembly, for example, and 2-10 light source, a 3-8 light source or 4-6 light source. In certain embodiments, described light source can comprise LED array. In this manner, single light source can comprise, for example, and from I to 50 LED, from 2 to 40 LED, from 3 to 30 LED, from 5 to 25 LED, from 6 to 20 LED, from 7 to 18 LED or from 8 to 16 LED.
In certain embodiments, described illuminator can comprise one or more can with all of described illuminator or some or optically focused and/or a light manipulation device that light source is associated. In certain embodiments, described optically focused and/or light manipulation device can be selected from wave filter, colour filter, neutral density filters, low pass filter, high-pass filter, bandpass filter, double-colored phase filter, mirror, double-colored mirror, lens, collimation lens, ripple plate, wave guide, prism, diffraction grating, baffle plate, beam splitter and combination thereof etc. In certain embodiments, described optically focused and/or light manipulation device can comprise luminous intensity control assembly. In certain embodiments, described luminous intensity control assembly can comprise neutral density filters or the voltage controller for controlling the voltage (light source emission intensity by this) for light source. In certain embodiments, the control intensity of light source has been improved the dynamic range of existing illuminator. In certain embodiments, at least one light source described in predeterminable putting, thereby make except automatic or manual excite or manual and automatic shooting on group described in illuminator, do not need system described in operation bidirectional, to provide sufficient irradiation for successfully catching image.
In certain embodiments, described system can comprise control and the communicating circuit system that can in processing procedure, catch the image capture system of sample image and described image be offered to described device. In certain embodiments, described image capture system can comprise digital camera, is wherein preferably the high accuracy number refrigeration CCD camera that carries cooling system. In certain embodiments, described image capture system comprises the digital camera that can produce the output image that is greater than 300DPI, for example 300DPI is to 3000DPI, for example 300DPI to 2600DPI, for example 400DPI to 2200DPI, 400DPI to 2000DPI, 500DPI is to 1600DPI, 500DPI is to 1200DPI, and 500DPI is to 800DPI or 500DPI to 600DPI. Should be appreciated that any applicable output image DPI is acceptable, for example, at least 300DPI. In certain embodiments, described image capture system for example, through arranging the image of catching that any applicable image file format can be provided .Pdf file, tiff file or bitmap file. Equally, the resolution ratio of image of being hunted down can be greater than approximately one mega pixel, for example, from approximately 1,000,000 pixels to approximately 6,000,000 pixels, from approximately 1,000,000 pixels to approximately 5,000,000 pixels, from approximately 1,100,000 pixels to approximately 4,500,000 pixels, from approximately 1,200,000 pixels to approximately 4,000,000 pixels, from approximately 1,300,000 pixels to approximately 3,500,000 pixels, from approximately 1,400,000 pixels to approximately 3,200,000 pixels, from approximately 1,500,000 pixels to approximately 3,000,000 pixels, from approximately 1,600,000 pixels to approximately 2,800,000 pixels, from approximately 1,700,000 pixels to approximately 2,500,000 pixels or from 1,800,000 pixels to approximately 2,200,000 pixels. Should be appreciated that any applicable image resolution ratio is all acceptable, for example, at least about the resolution ratio of 1,000,000 pixels. In certain embodiments, described image capture system can comprise one or more figure image focus or manipulated image device, for example, be selected from the device of wave filter, mirror, lens lamp etc.
In certain embodiments, described Full-automatic chemiluminescence imaging device comprises applicable integrated system display, integrated computer system or computer processor and an applicable integrated memory, with operation and storing software, and service data and meeting is controlled processing, imaging and analysis and inserts the parameter of the sample of described device. In certain embodiments, one or more realtime graphics that described display can show in diversity of settings image capture, image capture and analytic process. Described display can be any applicable display, as flat screen display or touch screen displays system. In certain embodiments, described display system can show have close to or the visual image size larger than actual sample size. Described display can once be exported multiple images or once export multiple images. Described display can comprise touch screen displays, and user can input for user alternately by described touch screen displays and described device, and the data such as such as voltage, electric current, power, time and form, to provide the manual control of described device or the manual input of code.
In certain embodiments, it is the stack of white light imaging and chemiluminescence imaging that described image split screen is shown as the 1st figure, and the 2nd to the 4th figure display gray scale scope increases progressively 1-3 doubly successively.
In certain embodiments, described Full-automatic chemiluminescence imaging device or software or hardware can provide, can comprise and maybe can meet one or more in many desired conditioned disjunction features. In certain embodiments, provide external communication interface, described external communication interface is connected with control and communicating circuit system. For example, can provide USB interface to support the use of USB dish. Can provide the support of USB device and file system interface to support being directly connected to PC (mass storage), can provide ftp server and file system interface with the transmission of network enabled file.
The present invention compared with prior art adopts integrated industrial design, effectively saves space. Full-automatic imaging control, one-key operation luminescence imaging operation: high-precision full-automatic exposure, a key is taken sample image, is automatically saved to USB flash disk, without setting time for exposure and camera lens parameters, simple to operate. Once take and can obtain multiple time for exposure effect image, and can be displayed in full screen or split screen shows multiple images simultaneously, conveniently observe contrast. Also possesses a key printing function, printable any design sketch.
Brief description of the drawings
Fig. 1 is the Full-automatic chemiluminescence imaging device structural representation the present invention relates to;
Fig. 2 is the chemiluminescence imaging device imaging flow chart the present invention relates to.
Fig. 3 is split screen demonstration figure.
Detailed description of the invention
Below in conjunction with specific embodiment, further set forth the present invention. These embodiment are only not used in and limit the scope of the invention for the present invention is described. The experimental technique of unreceipted actual conditions in the following example, the condition of conventionally advising according to normal condition or according to manufacturer. Unless otherwise defined, the familiar meaning of all specialties that use in literary composition and scientific words and one skilled in the art is identical. In addition, any method similar or impartial to described content and material all can be applicable in the inventive method. The use that better implementation method described in literary composition and material only present a demonstration.
A kind of brand-new Full-automatic chemiluminescence imaging system, comprise outer cabinet 1, camera bellows 2, power supply 3, Digital CCD Camera 4, control and USB communicating circuit 5, white light 6, objective table 7, USB interface 8, panel computer 9, button 10, it is characterized in that power supply 3 provides 12V dc source for whole device, white light 6 is light source, automatically calculate the reasonable time for exposure according to different sample software, control and USB communicating circuit 5 are controlled the duty of white light 6, gather picture signal and send to panel computer 9 by Digital CCD Camera 4, after processing, software is shown on panel computer 9, and be saved to USB flash disk by USB interface 8. see Fig. 1
Its flow process as shown in Figure 2, sample is placed in objective table, closes outer cabinet, by starting button, power supply energising, control and communicating circuit system initialization, detected image capture systems temperature, arranges shooting option, detection of stored system, illuminator is opened, automatic shooting, and the automatic exposure time is pressed following algorithm:
In formula: t0For the pre-exposure time, the gray scale array of its imaging is G, k=0.0333 (grey jump > 100) or k=0.0033 (grey jump≤100), m=Max (int (G × 10-2))，n＝Min(int(G×10-2))；
Take white light egative film, illuminator is closed, and then takes chemiluminescence picture, image stack or/or inverse processing, image single screen or split screen or Overlapping display, image storage, powered-down.
Split screen shows as Fig. 3, and the advantage that split screen shows is the sample drawing that can observe directly different gradation effect, has saved the trouble of the sample drawing of later stage manual handle. The 1st figure is the stack of white light imaging and chemiluminescence imaging, can know and show protein standard size. The the 2nd to the 4th figure display gray scale scope increases progressively 1,2 and 4 times successively, thereby obtains the image of different gray scales, more directly perceived, convenient, can meet client's actual needs.
If the real minimum gray of piece image is 500, maximum gray is 5000 words. When user has manually inputted the maximum gray 1000 showing in software interface. Second of the split screen demonstration GTG indication range to the 4th pictures is followed successively by so: 500-1000; 500-2000; 500-4000. 4 shown gradation effect of different gray scale images present multiplication difference, can meet most of users' needs, and without resetting.
Scope of the present invention is not subject to the restriction of described specific embodiments, and described embodiment, only as the single example of illustrating various aspects of the present invention, also comprises method and the component of functional equivalent in the scope of the invention. In fact,, except content as herein described, those skilled in the art can easily grasp multiple improvement of the present invention with reference to description and accompanying drawing above. Within described improvement also falls into the scope of appended claims. Every section of bibliography mentioned above is listed in herein as a reference all in full.
Claims (8)
1. a Full-automatic chemiluminescence imaging device, comprises outer cabinet, camera bellows, power supply, image capture systemSystem, control and communicating circuit system, illuminator, objective table, storage system, display system and startup button,Start by key control electric power starting, Power supply is to image capture system, control and communicating circuit system, storageSystem and illuminator, control and communicating circuit system connects respectively image capture system, storage system, demonstrationSystem and illuminator; It is characterized in that sample is placed in objective table, close outer cabinet, by starting button, power supplyEnergising, controls and communicating circuit system initialization, and detected image capture systems temperature, arranges shooting option, inspectionSurvey storage system, illuminator is opened, automatic shooting, and the automatic exposure time is pressed following algorithm:
In formula: t0For the pre-exposure time, the gray scale array of its imaging is G, k=0.0333 when grey jump > 100,Ash jump≤100 o'clock k=0.0033, m=Max (int (G × 10-2))，n＝Min(int(G×10-2))；
Take white light egative film, illuminator is closed, and then takes chemiluminescence picture, image stack or inverse placeReason, image single screen or split screen or Overlapping display, image storage, powered-down.
2. Full-automatic chemiluminescence imaging device as claimed in claim 1, is characterized in that described image dividesIt is the stack of white light imaging and chemiluminescence imaging that screen display is shown the 1st figure, taking the minimum gray of the 1st figure asRadix, the 2nd figure display gray scale scope is radix to 2 × radix, the 3rd figure display gray scale scope is radixTo 4 × radix, the 4th figure display gray scale scope is radix to 8 × radix.
3. Full-automatic chemiluminescence imaging device as claimed in claim 1, is characterized in that described illumination isSystem is white light LEDs.
4. Full-automatic chemiluminescence imaging device as claimed in claim 1, is characterized in that described image catchesThe system of obtaining comprises one or more image focusing arrangements.
5. Full-automatic chemiluminescence imaging device as claimed in claim 1, is characterized in that described image catchesThe system of obtaining is the high accuracy number cooling CCD system that carries cooling system.
6. Full-automatic chemiluminescence imaging device as claimed in claim 1, is characterized in that described demonstration isSystem is flat screen display or touch screen displays system.
7. Full-automatic chemiluminescence imaging device as claimed in claim 1, it is characterized in that it also comprises outsidePortion's communication interface, described external communication interface is connected with control and communicating circuit system.
8. Full-automatic chemiluminescence imaging device as claimed in claim 7, is characterized in that described outside is logicalCommunication interface is USB interface.
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| JPH10191183A (en) * | 1996-12-27 | 1998-07-21 | Fuji Photo Film Co Ltd | Image pickup device |
| JP3678397B2 (en) * | 1998-12-15 | 2005-08-03 | 富士写真フイルム株式会社 | Shooting system |
| CN1255671C (en) * | 2002-08-02 | 2006-05-10 | 上海数康生物科技有限公司 | Multiple-marknig-object biological chip signal analyzing systems |
| CN1223683C (en) * | 2002-12-16 | 2005-10-19 | 中国科学院光电技术研究所 | Biochip detecting system |
| CN2720435Y (en) * | 2004-08-03 | 2005-08-24 | 湖北资讯信息科技有限公司 | Chemical luminous digital imaging analyzing system |
| JP2011199659A (en) * | 2010-03-19 | 2011-10-06 | Fujifilm Corp | Imaging device and imaging program |
| EP2455891A1 (en) * | 2010-11-23 | 2012-05-23 | Synoptics Limited | Methods and systems for automatic capture of an image of a faint pattern of light emitted by a specimen |
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