CN103981232A - Preparation method of functional lactobacillus acidophilus peptidoglycan and anti-inflammatory activity use of functional lactobacillus acidophilus peptidoglycan - Google Patents
Preparation method of functional lactobacillus acidophilus peptidoglycan and anti-inflammatory activity use of functional lactobacillus acidophilus peptidoglycan Download PDFInfo
- Publication number
- CN103981232A CN103981232A CN201410156383.1A CN201410156383A CN103981232A CN 103981232 A CN103981232 A CN 103981232A CN 201410156383 A CN201410156383 A CN 201410156383A CN 103981232 A CN103981232 A CN 103981232A
- Authority
- CN
- China
- Prior art keywords
- peptidoglycan
- lactobacterium acidophilum
- functional
- lactobacillus acidophilus
- carrying
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a preparation method of a functional lactobacillus acidophilus peptidoglycan and an anti-inflammatory activity use of the functional lactobacillus acidophilus peptidoglycan. The preparation method comprises the following steps of inoculating a MRS culture medium with lactobacillus acidophilus having an accession number of CGMCC No. 8920 according to an inoculation ratio of 2.0-4.0% by volume, carrying out anaerobic cultivation at a temperature of 37 DEG C for 18-24h, carrying out centrifugation under a centrifugation force of 4000-8000g for 15-20min so that a fermentation broth is removed, adding 4wt% of a SDS solution into the precipitated acidophilus peptidoglycan, carrying out high-temperature pyrolysis at a temperature of 100 DEG C for 30min to remove a protein component, adding 10wt% of a trichloroacetic acid solution into the precipitates to remove teichoic acid, carrying out overnight dialysis desalination on the precipitates at a temperature of 4 DEG C, carrying out pre-refrigeration on the precipitates at a temperature of -80 DEG C for 6h, fast putting the refrigerated precipitates into a drying device, and carrying out drying treatment under the pressure of 80-120Pa for 24h to obtain a powdery product. The functional lactobacillus acidophilus peptidoglycan has good inhibition and conditioning effects on LPS-caused inflammation.
Description
Technical field
The present invention relates to the preparation method of peptidoglycan, especially relate to a kind of preparation method and anti-inflammatory activity application thereof of functional Lactobacterium acidophilum peptidoglycan.
Background technology
Milk-acid bacteria, as the important field planting type beneficial bacteria of intestinal tract of a class, is widely used in leavened food processing especially yogurt, cheese, pickles etc.The substantial connection of milk-acid bacteria and host health in intestines, is more and more set forth by microbiologist both at home and abroad, especially brings into play aspect its biological effect such as anti-infective, antitumor inducing about the release of immune regulatory factor carrys out stimulating immune system.Milk-acid bacteria to aspect host's immunoregulatory activity, research recently finds not have significant difference between some viable bacteria and dead bacterium, and one of reason of inferring its immunoregulation effect of performance may be the materials such as peptidoglycan (PGN), teichoic acid (LTA) and the S-layer proteins in its cell walls.Probiotic bacterium peptidoglycan, probes into relative shortage due to what it played a role in immunomodulatory, and just subtle is paid close attention to by increasing investigators.
Lactobacterium acidophilum (
lactobacillus acidophilus) as a kind of important beneficial bacteria of intestinal tract, its whole cell peptidoglycan is as a kind of immunostimulant, by regulating intestinal canal immunity system, can enhancing body non-specific immune function, body health is had to vital role.But the preparation method of Lactobacterium acidophilum peptidoglycan and anti-inflammatory activity research have no relevant report, on market, be also to occur with the relevant functional food of Lactobacterium acidophilum peptidoglycan.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of inflammatory reaction that LPS is caused and has preparation method and the anti-inflammatory activity application thereof of the functional Lactobacterium acidophilum peptidoglycan that presses down preferably scorching regulatory function.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of preparation method of functional Lactobacterium acidophilum peptidoglycan, specifically comprises the following steps: by the Lactobacterium acidophilum in source, ocean (
lactobacillus acidophilus) preserving number is CGMCC No.8920, the by volume inoculum size of per-cent 2.0-4.0%, be seeded on MRS substratum, at 37 DEG C, anaerobism is cultivated after 18-24h, in the centrifugal 15-20min of 4000-8000g, remove thalline fermented liquid, then precipitation being added to massfraction in thalline is 4% SDS solution, and Pintsch process 30min at 100 DEG C, removes protein ingredient; Taking precipitate, in throw out, adding massfraction is 10% trichoroacetic acid(TCA) solution, removes teichoic acid; Taking precipitate, after 4 DEG C of dialyzed overnight desalinations, after-80 DEG C of pre-freeze 6h, puts into rapidly vacuum freeze in 80-120Pa drying treatment 24h by throw out by throw out, and the powder obtaining is Lactobacterium acidophilum peptidoglycan.
The ratio of described precipitation thalline and described SDS solution is 1g:100ml, and the ratio of described precipitation thalline and described trichoroacetic acid(TCA) solution is 1g:100ml.
The anti-inflammatory activity application of above-mentioned functions Lactobacterium acidophilum peptidoglycan, but the inflammatory reaction that this functional Lactobacterium acidophilum peptidoglycan causes e. coli lipopolysaccharide (LPS) has preferably scorching regulatory function, be that Lactobacterium acidophilum peptidoglycan is inhibited to iNOS and COX-2 protein expression, on Raw264.7 scavenger cell model, effectively pressing down scorching concentration is 100-200 μ g/mL.
This functional Lactobacterium acidophilum peptidoglycan finds through immunity section, and this functional Lactobacterium acidophilum peptidoglycan effectively reduces the expression amount of iNOS small intestine intestines parietal cell, on mouse model effectively to press down scorching dosage be 100mg/kg/d.
Compared with prior art, the invention has the advantages that:
The preparation method of a kind of functional Lactobacterium acidophilum peptidoglycan of the present invention and anti-inflammatory activity application thereof, prove that first this Lactobacterium acidophilum peptidoglycan exists the inflammatory reaction that reduction e. coli lipopolysaccharide causes, for milk-acid bacteria regulating intestinal canal immunocompetence, the release of the regulation and control related immune factor carrys out stimulating immune system and brings into play that it is anti-infective, and the exploitation milk-acid bacteria new type of health factor, functional foodstuff or medicine provide theoretical foundation.
Lactobacterium acidophilum (
lactobacillus acidophilus), bacterial strain is WZ-D1, and preserving number is CGMCC No.8920, is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 03 14th, 2014, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Brief description of the drawings
Fig. 1 is Lactobacterium acidophilum thalline scanning electron microscope (SEM) photograph in embodiment 1;
Fig. 2 is the Lactobacterium acidophilum peptidoglycan scanning electron microscope (SEM) photograph of preparation in embodiment 1;
Fig. 3 is that the HPLC of Lactobacterium acidophilum peptidoglycan in embodiment 1 analyzes collection of illustrative plates;
Fig. 4 is Lactobacterium acidophilum peptidoglycan infrared scan spectrogram in embodiment 2;
Fig. 5 is the inhibiting Experimental Comparison figure of the RAW264.7 scavenger cell inflammatory factor of Lactobacterium acidophilum peptidoglycan to LPS induction in embodiment 3;
Fig. 6 is the Experimental Comparison figure of the expression amount difference of iNOS in the intestinal cells in different treatment group in embodiment 3 ICR mouse experiments; From left to right be followed successively by Normal group, pathogenic colon bacillus infected group, peptidoglycan treatment group.
Embodiment
Below in conjunction with accompanying drawing, embodiment is described in further detail the present invention.
Specific embodiment 1
The preparation of Lactobacterium acidophilum peptidoglycan
By the Lactobacterium acidophilum in source, ocean (
lactobacillus acidophilus) preserving number is the by volume inoculum size of per-cent 2.0-4.0% of CGMCC No.8920, be seeded on MRS substratum, at 37 DEG C, anaerobism is cultivated after 18-24h, in the centrifugal 15-20min of 4000-8000g, remove thalline fermented liquid, then getting precipitation thalline 1g, to add massfraction be 4% SDS solution, at 100 DEG C, Pintsch process 30min, removes protein ingredient; Taking precipitate, in throw out, adding massfraction is 10% trichoroacetic acid(TCA) solution, removes teichoic acid; Taking precipitate, after 4 DEG C of dialyzed overnight desalinations, after-80 DEG C of pre-freeze 6h, puts into rapidly vacuum freeze in 80-120Pa drying treatment 24h by throw out by throw out, and the powder obtaining is Lactobacterium acidophilum peptidoglycan.Sample is stored in-40 DEG C of refrigerators with for subsequent use.Wherein Fig. 1 is Lactobacterium acidophilum thalline scanning electron microscope (SEM) photograph, and Fig. 2 is the Lactobacterium acidophilum peptidoglycan scanning electron microscope (SEM) photograph of preparation.Lactobacterium acidophilum peptidoglycan is compared Lactobacterium acidophilum significant difference, and the form of ellipse bar is destroyed, and Fig. 2 is the peptidoglycan composition extracting.
The extraction yield of peptidoglycan calculates by following formula, and the peptidoglycan extraction yield that this example obtains is 26.5%.
Extraction yield (%)=(peptidoglycan dry weight/dry cell weight) × 100
The purity of peptidoglycan is by HPLC method validation, Agilent Agilent 1200 Infinity equipment, the Aminex HPX-87H sugar post (300 × 7.8 mm) of Bole company, sample size 20ul, sample introduction concentration 1mg/ml, 37 DEG C of column temperatures, elutriant is 5 mM sulphuric acid solns, flow velocity 0.5 ml/min, time 20min, detection method 225 nm ultraviolet spectrometries.As shown in Figure 3, the HPLC appearance time that derives from lactobacillus acidophilus cells's wall peptidoglycan is 7.618min to result.The peptidoglycan purity that this example obtains is 99.9%.
Purity (%)=(peptidoglycan go out peak area/detection sample always go out peak area) × 100
Above-mentioned Lactobacterium acidophilum (
lactobacillus acidophilus), bacterial strain is WZ-D1, and preserving number is CGMCC No.8920, is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on 03 14th, 2014, preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica.
Specific embodiment 2
The Lactobacterium acidophilum peptidoglycan that above-described embodiment 1 is prepared carries out mass spectrum (MALDI-TOF/TOF MS) and detects analysis; Lactobacterium acidophilum peptidoglycan powder carries out amino acid analysis and infrared (RI) detects.
1, the molecular weight of peptidoglycan and substructure
The Lactobacterium acidophilum peptidoglycan solution that is 1mg/ml by 1 microlitre concentration mixes with the DHB of 1 microlitre 50 mg/ml, after mixing, gets 1 microlitre mixed solution loading, carries out mass spectroscopy.Instrument model FLEX-PC, type is ultraflex TOF/TOF.The molecular weight of the detected peptidoglycan of this scheme is 875.260 Da, makes a concrete analysis of as shown in table 1 below.
Table 1 peptidoglycan ion fragment table
2, analysis of amino acids
Utilize A200 Amino Nova amino acidanalyser, analyze the amino acid whose composition of peptidoglycan and relative content height, thereby confirm amino acid whose composition in peptidoglycan.Concrete steps are as follows: 10mg peptidoglycan is hydrolyzed to 24h with the hydrochloric acid of 6M at 110 DEG C, and the product after hydrolysis is analyzed amino acid composition and the relative content in hydrolyzate through amino acidanalyser again.Result is as shown in table 2,
The relative content of main amino acid (concentration >=80 mg/g) in table 2 peptidoglycan
3, the characteristic group in Infrared spectrum scanning peptidoglycan sample
Peptidoglycan powdered sample is placed in to agate mortar even with KBr powder mixed grinding, is pressed into thin slice through tabletting machine, at wave number 4,000-400cm
-1carry out Infrared spectrum scanning, to determine characteristic group.Infrared scan spectrogram as shown in Figure 4,1070 cm
-1, there is C-O-C glycosidic link absorption peak in point bands of a spectrum region.1650 cm
-1, there is C=O stretching vibration, the superposition peak of carbonyl peak and COC in point bands of a spectrum region; 1540 cm
-1there are N-H flexural vibration in point bands of a spectrum region, amino peak (protonated amino) shows to exist in peptidoglycan amido linkage (peptide bond) functional group.Comprehensive above detection method, show that the structural models of Lactobacterium acidophilum muramyl peptide glycan is as follows,
。
Specific embodiment 3
The anti-inflammatory activity analysis of Lactobacterium acidophilum peptidoglycan prepared by above-described embodiment 1
(1) utilize the inflammatory model of the scavenger cell Raw 264.7 of Lactobacterium acidophilum peptidoglycan to LPS induction to regulate active, analyze iNOS in end product and the expression amount of COX-2 and change, analyze peptidoglycan and whether there is good inhibition Inflammatory substances and discharge active.
Six orifice plates are cultivated Raw 264.7 cells, and normal group is that adherent rate reaches the normal scavenger cell RAW264.7 of 60-80% cell, continue to extract cytoplasm protein after cultivation 18 h; LPS stimulating group is that in adherent rate reaches RAW 264.7 scavenger cells of 60-80%, to add final concentration be that the LPS of 100ng/ml induces, and extracts cytoplasm protein after continuing to cultivate 18 h; Peptidoglycan reparation group is that adherent rate reaches that in the scavenger cell of 60-80%, to add final concentration be the peptidoglycan that the LPS of 100ng/ml and final concentration are followed successively by the concentration gradient of 25 μ g/ml, 50 μ g/ml, 100 μ g/ml, 200 μ g/ml simultaneously, extracts cytoplasm protein after continuing to cultivate 18 h.
The albumen of different groups is carried out to protein immunoblotting analysis (Western Blot), target protein iNOS and COX-2 expressing quantity are analyzed.The restraining effect result of the RAW264.7 scavenger cell inflammatory factor of Lactobacterium acidophilum peptidoglycan to LPS induction as shown in Figure 5, for the cell that produces inflammatory reaction, the expression amount of its iNOS and COX-2 can be far away higher than blank group (left side first row in diagram), and the inflammatory model of finding its scavenger cell Raw 264.7 to LPS induction in peptidoglycan treatment group has significant adjusting activity, its activity is when PNG concentration is during at 100-200 μ g/ml, the significantly expression of inflammation-inhibiting factor iNOS and COX-2.Wherein glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is house keeping protein, and the expression amount in all cells is stable, uses as internal reference.
(2) ICR mouse experiment
60 suckling mouses of 25-28g ICR mouse (each 30 of male and female) are selected in experiment, are divided into 4 groups: Normal group, pathogenic colon bacillus infected group, peptidoglycan treatment group, 15 every group.Raise after 5 days, every of infected group gavages 0.50mL pathogenic colon bacillus liquid (5 × 10 with the syringe per os of improveing
8cFU/ml); Treatment group gavages 0.50mL pathogenic colon bacillus liquid (5 × 10 on the 5th day
8cFU/ml), after 1h, gavage Lactobacterium acidophilum peptidoglycan suspension liquid with the dosage of 100mg/kg/d, play the peptidoglycan that gavages same dose every day next day; Normal control is raised after 5 days and is gavaged 0.50mL physiological saline.After 25 days, mouse is implemented to euthanasia, the immunity section of carrying out intestinal cell detects the expression amount of iNOS in different groups.As shown in Figure 6, shown spot in figure, is the inflammatory factor iNOS that confirms by the immunization method expression amount in mouse intestinal cell to result, and in the mouse intestinal cell of gavage peptidoglycan, the expression amount of iNOS is starkly lower than pathogenic colon bacillus treatment group.Visible, also absolutely prove by mouse experiment the effect that peptidoglycan plays in enteron aisle anti-inflammatory.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited to above-mentioned giving an example.Variation, remodeling, interpolation or replacement that those skilled in the art make in essential scope of the present invention, also should belong to protection domain of the present invention.
Claims (4)
1. a preparation method for functional Lactobacterium acidophilum peptidoglycan, is characterized in that specifically comprising the following steps: by the Lactobacterium acidophilum in source, ocean (
lactobacillus acidophilus) preserving number is CGMCC No.8920, the by volume inoculum size of per-cent 2.0-4.0%, be seeded on MRS substratum, at 37 DEG C, anaerobism is cultivated after 18-24h, in the centrifugal 15-20min of 4000-8000g, remove thalline fermented liquid, then precipitation being added to massfraction in thalline is 4% SDS solution, and Pintsch process 30min at 100 DEG C, removes protein ingredient; Taking precipitate, in throw out, adding massfraction is 10% trichoroacetic acid(TCA) solution, removes teichoic acid; Taking precipitate, after 4 DEG C of dialyzed overnight desalinations, after-80 DEG C of pre-freeze 6h, puts into rapidly vacuum freeze in 80-120Pa drying treatment 24h by throw out by throw out, and the powder obtaining is Lactobacterium acidophilum peptidoglycan.
2. the preparation method of a kind of functional Lactobacterium acidophilum peptidoglycan according to claim 1, it is characterized in that: the ratio of described precipitation thalline and described SDS solution is 1g:100ml, the ratio of described precipitation thalline and described trichoroacetic acid(TCA) solution is 1g:100ml.
3. the anti-inflammatory activity of a functional Lactobacterium acidophilum peptidoglycan according to claim 1 application, it is characterized in that: but the inflammatory reaction that this functional Lactobacterium acidophilum peptidoglycan causes e. coli lipopolysaccharide (LPS) has preferably scorching regulatory function, be that Lactobacterium acidophilum peptidoglycan is inhibited to iNOS and COX-2 protein expression, on Raw264.7 scavenger cell model, effectively pressing down scorching concentration is 100-200 μ g/mL.
4. the anti-inflammatory activity of a functional Lactobacterium acidophilum peptidoglycan according to claim 1 application, it is characterized in that: this functional Lactobacterium acidophilum peptidoglycan is found through immunity section, this functional Lactobacterium acidophilum peptidoglycan effectively reduces the expression amount of iNOS small intestine intestines parietal cell, on mouse model effectively to press down scorching dosage be 100mg/kg/d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410156383.1A CN103981232B (en) | 2014-04-18 | 2014-04-18 | The preparation method of a kind of functional bacillus acidophilus's Peptidoglycan and anti-inflammatory activity application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410156383.1A CN103981232B (en) | 2014-04-18 | 2014-04-18 | The preparation method of a kind of functional bacillus acidophilus's Peptidoglycan and anti-inflammatory activity application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103981232A true CN103981232A (en) | 2014-08-13 |
| CN103981232B CN103981232B (en) | 2016-08-17 |
Family
ID=51273404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410156383.1A Active CN103981232B (en) | 2014-04-18 | 2014-04-18 | The preparation method of a kind of functional bacillus acidophilus's Peptidoglycan and anti-inflammatory activity application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103981232B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107296097A (en) * | 2017-06-12 | 2017-10-27 | 宁波大学 | A kind of feature flower taste fermentation milk powder and its processing method |
| CN109553662A (en) * | 2018-11-02 | 2019-04-02 | 宁波大学 | A kind of protein-bonded expression and purification of functionality lactobacillus acidophilus mucus and its application |
| CN115068510A (en) * | 2022-05-24 | 2022-09-20 | 宁波大学 | Extraction method of lactobacillus lipoteichoic acid and anti-inflammatory activity application thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101144062A (en) * | 2006-09-15 | 2008-03-19 | 内蒙古农业大学 | Lactobacillus casei strain and application for products thereof in bird immunity |
| CN101717806A (en) * | 2009-12-03 | 2010-06-02 | 内蒙古双奇药业股份有限公司 | Integrated peptidoglycan and preparation method thereof |
| CN103396496A (en) * | 2013-07-16 | 2013-11-20 | 宁波大学 | Preparation method of modified lactobacillus acidophilus peptidoglycan |
-
2014
- 2014-04-18 CN CN201410156383.1A patent/CN103981232B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101144062A (en) * | 2006-09-15 | 2008-03-19 | 内蒙古农业大学 | Lactobacillus casei strain and application for products thereof in bird immunity |
| CN101717806A (en) * | 2009-12-03 | 2010-06-02 | 内蒙古双奇药业股份有限公司 | Integrated peptidoglycan and preparation method thereof |
| CN103396496A (en) * | 2013-07-16 | 2013-11-20 | 宁波大学 | Preparation method of modified lactobacillus acidophilus peptidoglycan |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107296097A (en) * | 2017-06-12 | 2017-10-27 | 宁波大学 | A kind of feature flower taste fermentation milk powder and its processing method |
| CN109553662A (en) * | 2018-11-02 | 2019-04-02 | 宁波大学 | A kind of protein-bonded expression and purification of functionality lactobacillus acidophilus mucus and its application |
| CN109553662B (en) * | 2018-11-02 | 2021-07-13 | 宁波大学 | Expression and purification of functional lactobacillus acidophilus mucus binding protein and application thereof |
| CN115068510A (en) * | 2022-05-24 | 2022-09-20 | 宁波大学 | Extraction method of lactobacillus lipoteichoic acid and anti-inflammatory activity application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103981232B (en) | 2016-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101772870B1 (en) | Novel Lactobacillus plantarum with probiotic activities and use thereof | |
| WO2022206300A1 (en) | Composition capable of facilitating defecation and use thereof | |
| KR101997362B1 (en) | Lactobacillus plantarum BK-021 or anti-obesity composition comprising thereof | |
| CN110564638A (en) | Lactobacillus reuteri with probiotic characteristics and application thereof | |
| CN114601174B (en) | Lactic acid bacteria lysate and preparation method and application thereof | |
| CN117568211B (en) | Lactobacillus plantarum GOLDGUT-LP618 with salmonella infection resisting function and application thereof | |
| CN1916028A (en) | Polysaccharide outside cyst of lactobacillus casei and crude product, prepartion method and application | |
| CN114774315B (en) | Application of lactobacillus rhamnosus strain LRa05 in preparation of immunity enhancing product and/or eczema relieving product | |
| CN103981232A (en) | Preparation method of functional lactobacillus acidophilus peptidoglycan and anti-inflammatory activity use of functional lactobacillus acidophilus peptidoglycan | |
| CN116083326A (en) | Preparation containing bacillus coagulans metaplasium, preparation method thereof and application thereof in preparation of weight-losing and lipid-lowering drugs | |
| CN117089505B (en) | Lactobacillus paracasei VB306 and application thereof | |
| KR20180075463A (en) | Novel Lactobacillus fermentum with probiotic activities and use thereof | |
| KR101772875B1 (en) | Novel Lactobacillus plantarum with probiotic activities and use thereof | |
| CN116790402B (en) | Bacteroides simplex strain with anti-inflammatory property, culture method and application | |
| CN117286045B (en) | Bifidobacterium longum subspecies longum KS2 and application thereof in preparation of anti-aging medicines | |
| KR20130084887A (en) | Fermentation product of schisandra chinensis and antibacterial and immune enhancing composition comprising the same | |
| JP2020100660A (en) | Lactic acid bacteria, innate immunity activator derived from lactic acid bacteria, preventive and therapeutic agent for infectious diseases, and foods and drinks | |
| KR101772872B1 (en) | Novel Lactobacillus plantarum with probiotic activities and use thereof | |
| CN113773985A (en) | Bacillus subtilis for improving traditional Chinese medicine polysaccharide yield and regulating skin barrier and immunity | |
| CN117384790B (en) | Pediococcus pentosaceus KS5 and application thereof in preparation of sleep-aiding drugs | |
| CN116656526B (en) | Lactobacillus plantarum JF4 and application thereof in preparation of blood sugar and cholesterol reducing foods and medicines | |
| CN116574634B (en) | Streptococcus salivarius thermophilus subspecies JF2 and application thereof in preparation of anti-inflammatory and lipid-relieving food and drug | |
| KR102202092B1 (en) | Weissella koreensis nd824 strain having improved productivity of ornithine and composition comprisingng the same | |
| CN118109345A (en) | Enterococcus faecalis XY3 and application thereof in preparation of anti-inflammatory and sleep-aiding foods and medicines | |
| WO2014206279A1 (en) | Lactic acid bacteria and preparation method and use of fermented grains or grain germ extracts |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201026 Address after: One of the 3rd floor of No.2 factory building, No.96 Baiyun Avenue, Tong'an District, Xiamen City, Fujian Province Patentee after: Xiamen jinhailai Food Technology Co.,Ltd. Address before: 315211 Zhejiang Province, Ningbo Jiangbei District Fenghua Road No. 818 Patentee before: Ningbo University |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220825 Address after: Room 303, No. 96-1, Baiyun Avenue, Xike Town, Tong'an District, Xiamen City, Fujian Province 361000 Patentee after: Xiamen Fuyuankang Industrial Co.,Ltd. Address before: 361000 one of the 3rd floor of No.2 workshop, No.96 Baiyun Avenue, Tong'an District, Xiamen City, Fujian Province Patentee before: Xiamen jinhailai Food Technology Co.,Ltd. |