CN103981166B - The alkaline pectin enzyme mutant that a kind of thermotolerance improves - Google Patents

The alkaline pectin enzyme mutant that a kind of thermotolerance improves Download PDF

Info

Publication number
CN103981166B
CN103981166B CN201410191532.8A CN201410191532A CN103981166B CN 103981166 B CN103981166 B CN 103981166B CN 201410191532 A CN201410191532 A CN 201410191532A CN 103981166 B CN103981166 B CN 103981166B
Authority
CN
China
Prior art keywords
alkaline
sudden change
gene
thermotolerance
alkaline pectase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201410191532.8A
Other languages
Chinese (zh)
Other versions
CN103981166A (en
Inventor
陈坚
堵国成
刘松
汪明星
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangnan University
Original Assignee
Jiangnan University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Jiangnan University filed Critical Jiangnan University
Priority to CN201410191532.8A priority Critical patent/CN103981166B/en
Publication of CN103981166A publication Critical patent/CN103981166A/en
Application granted granted Critical
Publication of CN103981166B publication Critical patent/CN103981166B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02002—Pectate lyase (4.2.2.2)

Abstract

The invention discloses the alkaline pectin enzyme mutant that a kind of thermotolerance improves, belong to technical field of bioengineering.Will the present invention come from Bacillus? the Isoleucine I point mutation of the 325th of the alkaline pectase of sp.WSHB04-02 is phenylalanine F.Suddenly change after rear 50 DEG C of insulation 2h, residual enzyme work is 4.27 times before sudden change; The thermotolerance of alkaline pectase I325F at 50 DEG C after this illustrates sudden change significantly improves, and I325F can the maintenance rock steady structure of long period at 50 DEG C.The transformation period of alkaline pectase I325F at 55 DEG C after sudden change is 1.89 times before sudden change; K m, V max, k catand k cat/ K mdeng being all improved.The zymologic property of PGL provided by the invention is improved largely, and is more applicable to industrial production demand, meets the requirement of social production.

Description

The alkaline pectin enzyme mutant that a kind of thermotolerance improves
Technical field
The present invention relates to the alkaline pectin enzyme mutant that a kind of thermotolerance improves, belong to technical field of bioengineering.
Background technology
Alkaline pectase (E.C.4.2.2.2 is called for short PGL), full name galacturonan lyase, in the basic conditions there is high reactivity, trans-elimination can be utilized to cut off the α-Isosorbide-5-Nitrae-glycosidic link of poly lactose aldehydic acid, pectin substance is decomposed into undersaturated oligogalacturonans.This enzyme is extensively present in bacterium, yeast, fungi, plant and and some parasitic nematode.The alkaline pectase homology difference of different sources is larger.Alkaline pectase is widely used in the every field such as food, weaving, papermaking, environment, biotechnology, extracts at tea and coffee fermentation, weaving and vegetable fibre processing, oil, plays a great role containing pectin Industrial Wastewater Treatment etc.
A lot of accompanying impurities is contained in the most surface of cotton fibre primary cell wall, as: the non-cellulose class materials such as pectin substance, wax, nitrogenous substances and protein, mutual inclusion becomes complicated huge hydrophobic reticulated structure.Comprise refinement step in textile industry, its object is exactly remove the impurity on cotton fibre, improves the moisture absorbing of cotton fabric, then carries out following process.Traditional chemistry weaving method of refining instant heating alkaline process, it needs to consume a large amount of chemical and water resources, and environmental pollution is serious, and according to statistics, in dyeing process, the waste water of about 70% produces in this step.In addition, hot alkali treatment will cause major injury to fiber, and physical strength will decline to a great extent.
In recent ten years, the swift and violent fast development studied along with molecular biology and industrial microorganism, investigator starts to adopt genetic engineering means to build recombinant bacterium, to realizing the excessive recombinant expressed of alkaline pectase.At present, in succession there is the report utilizing Escherichiacoli, Bacillussubtilis and Pichiapastoris etc. as host expresses different sources polygalacturonase gene.Now synthesis alkaline pectase engineering bacteria and fermentation processes are mainly concentrated on to the research of alkaline pectase.Although the expression amount of PGL is very high, but the enzymatic properties such as the catalytic efficiency thermotolerance of enzyme need further improvement, and different hosts heterogenous expression is also just attempted in current done molecular modification, improve the research of the zymologic property aspects such as enzyme work, thermotolerance, catalytic efficiency, at present domestic do very few.Nearly ten years, the research direction of external alkaline pectase focuses on separation and purification, the aspect such as enzymatic property and protein structure properties study thereof of enzyme, China mainly concentrates on the improvement aspect of strain improvement, zymotechnique and application art to the research of alkaline pectase, and China still lacks the commodity alkaline pectase of tiring very high, for realizing industrialization place mat further, the research of this respect can not be ignored.
Summary of the invention
First technical problem that the present invention will solve is to provide the alkaline pectin enzyme mutant that a kind of thermotolerance improves.Described mutant is based on the alkaline pectase of aminoacid sequence as shown in SEQIDNO.2 (wild-type), is phenylalanine F by the Isoleucine I point mutation of the 325th.
Second technical problem that the present invention will solve is to provide the method building described alkaline pectin enzyme mutant, is to express after carrying out rite-directed mutagenesis by the gene of design primer pair encodes alkaline polygalacturonase.
Described construction process, be preferably that template design primer is carried out suddenling change and the Plastid transformation after sudden change is entered E.coliJM109 and increases with plasmid pET-20b (+)-pgl, then express the recombinant plasmid containing mutator gene with E.coliBL21 (DE3), obtain the alkaline pectin enzyme mutant that thermostability strengthens.
The construction process of described pET-20b (+)-pgl is see document: Overproductionofalkalinepolygalacturonatelyaseinrecombin antEscherichiacolibyatwo-stageglycerolfeedingapproach.20 11, ShuyingFangetal.Volume102, Issue22, p10671 – 10678.
The described primer for rite-directed mutagenesis is:
pglI325Fprimer1：5'-CTATGCCCAAAACAATGTCTTCGACGTACCGGGACTGTCAG-3'
pglI325Fprimer2：5'-CTGACAGTCCCGGTACGTCGAAGACATTGTTTTGGGCATAG-3'。
The 3rd technical problem that the present invention will solve is to provide the method for alkaline pectin enzyme mutant described in using gene engineering bacterium fermentative production, is to be inoculated into containing 100 μ gmL after recombinant bacterium E.coliBL21 (DE3) (pET-20b (+)-pglI325F) activation culture by the gene containing encoding mutant alkaline pectase -1in the fermention medium of penbritin, liquid amount is 50mL/500mL, in 37 DEG C, and 200rmi n-1cultivate; Thalli growth is to OD 600=0.6, add final concentration 0.4mMIPTG and induce, temperature is adjusted to 30 DEG C simultaneously, induction fermentation 48h.
Described activation culture is inoculated in recombinant bacterium E.coliBL21 (DE3) (pET-20b (+)-pglI325F) containing 100 μ gmL -1in the seed culture medium of penbritin, liquid amount is 20mL/250mL, culture temperature 37 DEG C, 200rpmmin -1shaking culture 10h on shaking table.
Described seed culture medium composition (g/L): yeast powder 5, Tryptones 10, NaCl10, glucose 20, pH7.0.
Described fermention medium composition: yeast powder 24g/L, Tryptones 12g/L, glycerine 5g/L, K 2hPO 472mmolL -1, KH 2pO 417mmolL -1.
The present invention, by Fixedpoint mutation modified alkaline pectinase gene, makes coded PGL thermotolerance strengthen; Before comparing sudden change, be incubated 24h through 30 DEG C, 50 DEG C of insulation 2h, after being incubated 1h at 55 DEG C, alkaline pectase (I325F) residual enzyme after sudden change increases before living and comparatively suddenling change.After 50 DEG C of insulation 2h, the residual enzyme work after sudden change is 4.27 times before sudden change; The thermotolerance of alkaline pectase I325F at 50 DEG C after this illustrates sudden change significantly improves, and I325F can the maintenance rock steady structure of long period at 50 DEG C.After sudden change, optimal reactive temperature improves 5 DEG C, and the rear alkaline pectase of sudden change can show optimum enzyme better under the high temperature conditions and live; The transformation period of alkaline pectase I325F at 55 DEG C after sudden change is 1.89 times before sudden change; K m, V max, k catand k cat/ K mdeng being all improved.Alkaline pectase provided by the invention is applicable to industrial production demand more, meets the requirement of social production.
Accompanying drawing explanation
Fig. 1 is the stability under the mutant strain that builds of the present invention and original strain differing temps, WT: the enzyme before sudden change; I325F: mutant.
Fig. 2 is the mutant strain that builds of the present invention and original strain structural changes, WT: the enzyme before sudden change; I325F: mutant.
Embodiment
Alkaline pectase enzyme activity detects: get a certain amount of fermented liquid, the centrifugal 10min of 8000rpm, and namely the outer alkaline pectase of born of the same parents is contained among fermented supernatant fluid.Alkaline pectase reaction system is: enzyme diluent 20 μ L, containing the glycine-NaOH buffer (0.2molL of 0.2%PGA -1, 0.44mmolL -1caCl 2, pH9.4) and 2mL, the enzyme liquid of non-activity is blank.Alkaline pectase reaction conditions is: 45 DEG C of water-bath 15min, uses 3mL phosphoric acid solution (0.03molL -1) termination reaction, 235nm place assaying reaction thing absorbance.
Alkaline pectase enzyme live unit definition for: within 1 minute, cracking polygalacturonic acid produces enzyme amount corresponding to 1 μm of ol unsaturated polyester galacturonic acid.
Alkaline pectase enzyme method of calculation alive:
(OD 235× 10 6× system volume × extension rate)/(10 3time of enzymatic reacting in × cuvette thickness × 4600 × enzyme reaction linearity range × enzyme liquid amasss)
Alkaline pectase purification condition: A liquid: glycine-NaOH, pH7.5; B liquid: glycine-NaOH, 2M ammonium sulfate, pH7.5; 5mL drainage column [HiTrapPhenvl (highsub) FF], 3mL/min, 30%B liquid wash-out obtains target protein; 30kDamillipore ultra-filtration centrifuge tube concentrates, and it is pure that dialysis obtains SDS-PAGE electrophoresis.
Alkaline pectase heat stability test: measure enzyme activity according to standard method at different temperatures, to process, preferment is alive is decided to be 100%., by enzyme liquid respectively at 30 DEG C of insulation 24h, 40 DEG C of insulation 2h, 50 DEG C of insulation 2h, are incubated 1h at 55 DEG C, after utilizing ice bath to cool rapidly, survey residual enzyme activity as stated above, investigate its thermotolerance.
Alkaline pectase K m, V maxdetect: measure under the concentration of substrate that 0.05-2g/L does not wait, predicted obtaining by GraphPadPrism5.
The structure of embodiment 1 mutation expression plasmid and the acquisition of recombined bacillus subtilis
1, with pET-20b (+)-pgl plasmid be template build mutant expression vector
Encoding wild type alkaline pectase and the nucleotide sequence of the gene of signal peptide that is made up of 21 amino acid are as shown in SEQIDNO.1, and the aminoacid sequence of wild-type mature alkaline pectase is as shown in SEQIDNO.2.By the heat-resisting polygalacturonase sequence of comparison: the CAD56882 of Bacilluslicheniformis, from the BAA96478 of Bacillussp.strainP-4-N, and from the AAD35518 of ThermotogamaritimaMSB82, optimum temperuture is respectively 69 DEG C, 70 DEG C and 90 DEG C.In conjunction with the three-dimensional structure of alkaline pectase, infer that the thermostability of Isoleucine I to alkaline pectase of 325 has considerable influence, design mutating experiment, is mutated into the same amino acid of other three heat-resisting sequences by the Isoleucine I of 325---phenylalanine F.
With pET-20b (+)-pgl plasmid for template, the Isoleucine I of the 325th is sported phenylalanine F by design primer.Primer for rite-directed mutagenesis:
pglI325Fprimer1：5'-CTATGCCCAAAACAATGTCTTCGACGTACCGGGACTGTCAG-3'
pglI325Fprimer2：5'-CTGACAGTCCCGGTACGTCGAAGACATTGTTTTGGGCATAG-3'。
PCR reaction system is as follows: (primer concentration is 20 μm of ol/L)
PCR reaction conditions: 94 DEG C of denaturation 10min, 98 DEG C of sex change 10s, 55 DEG C of annealing 15s, 72 DEG C extend 5min, 30 rear 72 DEG C of extension 10min of circulation, 4 DEG C of preservations.DpnI endonuclease is utilized to carry out digestions DNA profiling mutant expression vector pET-20b (+)-pglI325F obtained by PCR.
The construction process of plasmid pET-20b (+)-pgl is see document: Overproductionofalkalinepolygalacturonatelyaseinrecombin antEscherichiacolibyatwo-stageglycerolfeedingapproach.20 11, ShuyingFangetal.Volume102, Issue22, p10671 – 10678.
2, the conversion of mutant expression vector
(1) preparation of competent cell
By E.coliBL21 (DE3) picking list bacterium colony on streak plate substratum, be inoculated in LB substratum.37 DEG C of vibrations (200rpm) are cultivated.Measure OD 600value, works as OD 600(about cultivate 5 hours) when value reaches 0.35 ~ 0.5 to place in ice and stop cultivating (if OD 600value exceeds the transformation efficiency that this scope can not ensure competent cell).Get above-mentioned bacterial culture fluid 1ml (amount determines Microtube quantity as required) in 1.5mlMicrotube.1,500 × g (general microcentrifuge is about 4000rpm) 4 DEG C is centrifugal 5 minutes, abandons supernatant (noting as far as possible eliminating supernatant).The SolutionA of precooling in 100l ice is added in each Microtube, springing Microtube makes precipitation suspend gently, forbid thermal agitation 1,500 × g (general microcentrifuge is about 4000rpm) 4 DEG C is centrifugal 5 minutes, abandons supernatant (noting as far as possible eliminating supernatant).In each Microtube, add the SolutionB of precooling in 100 μ L ice, springing Microtube makes precipitation suspend gently, forbids thermal agitation.
(2) the checking amplification of mutant expression vector
Competent cell completes.This competent cell can be directly used in the transformation experiment of DNA, also can preserve in-80 DEG C, in order to using later.When preserving for-80 DEG C, can effectively preserve more than 1 year, but can not multigelation, once after melting ,-80 DEG C of preservations can not be carried out again.The DNA of competent cell transforms and the competent cell that-80 DEG C are preserved is placed in ice thawing 10 minutes.The competent cell getting 100 μ L moves in new conversion tube.In competent cell, add the conversion DNA of 0.1ng ~ 10ng (3 μ L ~ 10 μ L), place 30 minutes in ice after mixing gently.After placing for 90 seconds in 42 DEG C of water-baths, place 1 ~ 2 minute in ice immediately.Add the LB substratum of 890 μ L37 DEG C of pre-temperature.37 DEG C of shaking culture 1 hour.After getting appropriate painting flat board, flat-plate inverted is placed in 37 DEG C of incubators and cultivates a night.Confirm to cultivate bacterium colony, carry out lower step experiment.
Embodiment 2 is suddenlyd change the expression of PGL
Seed culture medium composition (g/L): yeast powder 5, Tryptones 10, NaCl10, glucose 20, pH7.0.
Fermention medium forms: yeast powder 24g/L, Tryptones 12g/L, glycerine 5g/L, K 2hPO 472mmolL -1, KH 2pO 417mmolL -1.
From glycerine pipe, the recombinant bacterium E.coliBL21 (DE3) containing mutant expression vector pET-20b (+)-pglI325F is inoculated in containing 100 μ gmL -1in the seed culture medium of penbritin, liquid amount is 20mL/250mL.Culture temperature 37 DEG C, shaking culture 10h on 200rpm shaking table.
The seed liquor of cultivating 10h is contained 100 μ gmL with the access of the inoculum size of 3% (V/V) -1in the fermention medium of penbritin, liquid amount is 50mL/500mL, in 37 DEG C, and 200rmin -1cultivate.Thalli growth is to certain phase (OD 600=0.6), add final concentration 0.4mMIPTG and induce, temperature is adjusted to 30 DEG C simultaneously, induction fermentation 48h.
The zymologic property of PGL before and after embodiment 3 sudden change
According to the method described in embodiment 2 respectively to ferment containing without the E.coliBL21 (DE3) of expression vector pET-20b (+)-pgl of sudden change and mutant strain E.coliBL21 (DE3) (pET-20b (+)-pglI325F), carry out the characterization analysis such as thermotolerance by after the enzyme purification in fermented liquid.Gained alkaline pectin enzyme mutant called after I325F.
As seen from Figure 1, before sudden change, alkaline pectase (WT) is being incubated 24h through 30 DEG C, 50 DEG C of insulation 2h, and after being incubated 1h at 55 DEG C, enzyme loss alive is obvious; After alkaline pectase (I325F) after sudden change does same treatment, residual enzyme increases before living and comparatively suddenling change, especially 50 DEG C of insulation 2h.After 50 DEG C of insulation 2h, the residual enzyme work after sudden change is 4.27 times before sudden change; The thermotolerance of alkaline pectase I325F at 50 DEG C after this illustrates sudden change significantly improves, and I325F can the maintenance rock steady structure of long period at 50 DEG C.
As can be seen from Table 1, after sudden change, optimal reactive temperature improves 5 DEG C, and the rear alkaline pectase of sudden change can show optimum enzyme better under the high temperature conditions and live; The transformation period of alkaline pectase I325F at 55 DEG C after sudden change is 1.89 times before sudden change; K m, V max, k catand k cat/ K mdeng there being a little improvement.After sudden change is described, the overall zymologic property of alkaline pectase all improves.
Table 1 mutant strain and original strain zymetology parameter
Analyze the structural changes of I325F, find that the InteractionAromatic-Aromatic (aromatic series reactive force) in I325F is increased to 28 to (331TRP-346PHE) by previous 27 couple, DiscoveryStudio2.5 structural analysis shows, 346ILE is replaced to PHE, directly results in the formation (Fig. 2) of new key.Can find out in the drawings, TRP and PHE two amino acid whose phenyl ring are very close, and it is more tight that sudden change makes this region connect than before.Thermotolerance stability, simultaneously with substrate be obtained for large increase in conjunction with stability.
Result shows, by sequence alignment and structure combination at critical sites mutating acid, this enzyme zymologic property can be made obviously to promote, and this strategy can be widely used in the improvement of zymologic property.
Although the present invention with preferred embodiment openly as above; but it is also not used to limit the present invention, any person skilled in the art, without departing from the spirit and scope of the present invention; all can do various changes and modification, what therefore protection scope of the present invention should define with claims is as the criterion.

Claims (10)

1. the alkaline pectin enzyme mutant that improves of thermotolerance, it is characterized in that, be phenylalanine F by the 325th of the alkaline pectase of aminoacid sequence as shown in SEQIDNO.2 the Isoleucine I point mutation.
2. the gene of mutant described in coding claim 1.
3. carry carrier or the cell of gene described in claim 2.
4. obtaining a method for alkaline pectin enzyme mutant described in claim 1, is express after carrying out rite-directed mutagenesis by the gene of design primer pair encodes alkaline polygalacturonase.
5. method according to claim 4, it is characterized in that, be that template design primer is carried out suddenling change and the Plastid transformation after sudden change is entered E.coliJM109 and increases with plasmid pET-20b (+)-pgl, then express the recombinant plasmid containing mutator gene with E.coliBL21 (DE3), obtain the alkaline pectin enzyme mutant that thermotolerance strengthens.
6. method according to claim 5, is characterized in that, the primer for rite-directed mutagenesis is:
pglI325Fprimer1：5'-CTATGCCCAAAACAATGTCTTCGACGTACCGGGACTGTCAG-3'
pglI325Fprimer2：5'-CTGACAGTCCCGGTACGTCGAAGACATTGTTTTGGGCATAG-3'。
7. the method for alkaline pectin enzyme mutant described in using gene engineering bacterium fermentative production claim 1, is characterized in that, is to be inoculated into after the recombinant bacterium activation culture containing sudden change alkaline pectinase gene containing 100 μ gmL -1in the fermention medium of penbritin, in 37 DEG C, 200rmin -1cultivate; Thalli growth is to OD 600=0.6, add IPTG and induce, temperature is adjusted to 30 DEG C simultaneously, induction fermentation 48h.
8. method according to claim 7, is characterized in that, described activation culture is inoculated in the recombinant bacterium E.coliBL21 (DE3) containing pET-20b (+)-pglI325F containing 100 μ gmL -1in the seed culture medium of penbritin, culture temperature 37 DEG C, shaking culture 10h on 200rpm shaking table.
9. method according to claim 8, is characterized in that, described seed culture medium composition: yeast powder 5g/L, Tryptones 10g/L, NaCl10g/L, glucose 20g/L, pH7.0.
10. method according to claim 7, is characterized in that, described fermention medium composition: yeast powder 24g/L, Tryptones 12g/L, glycerine 5g/L, K 2hPO 472mmolL -1, KH 2pO 417mmolL -1.
CN201410191532.8A 2014-05-07 2014-05-07 The alkaline pectin enzyme mutant that a kind of thermotolerance improves Expired - Fee Related CN103981166B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410191532.8A CN103981166B (en) 2014-05-07 2014-05-07 The alkaline pectin enzyme mutant that a kind of thermotolerance improves

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410191532.8A CN103981166B (en) 2014-05-07 2014-05-07 The alkaline pectin enzyme mutant that a kind of thermotolerance improves

Publications (2)

Publication Number Publication Date
CN103981166A CN103981166A (en) 2014-08-13
CN103981166B true CN103981166B (en) 2016-04-13

Family

ID=51273332

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410191532.8A Expired - Fee Related CN103981166B (en) 2014-05-07 2014-05-07 The alkaline pectin enzyme mutant that a kind of thermotolerance improves

Country Status (1)

Country Link
CN (1) CN103981166B (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106636049B (en) * 2016-12-27 2019-11-08 江南大学 A kind of alkaline pectin enzyme mutant that secernment property improves

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1662650A (en) * 2002-05-14 2005-08-31 诺和酶股份有限公司 Pectate lyase variants
CN103215244A (en) * 2013-04-28 2013-07-24 中国科学院天津工业生物技术研究所 Alkaline pectinase PelN, as well as encoded gene and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1662650A (en) * 2002-05-14 2005-08-31 诺和酶股份有限公司 Pectate lyase variants
CN103215244A (en) * 2013-04-28 2013-07-24 中国科学院天津工业生物技术研究所 Alkaline pectinase PelN, as well as encoded gene and application thereof

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Improvement of the Thermostability and Activity of a Pectate Lyase by Single Amino Acid Substitutions, Using a Strategy Based on Melting-Temperature-Guided Sequence Alingment;Zhizhuang Xiao, et al.;《Applied AND Environmental Microbiology》;20080228;第74卷(第4期);摘要，图1 *
Overproduction of alkaline polygalacturonate lyase in recombinant Esherichia coli by a two-stage glycerol feeding approach;Shuying Fang, et al.;《Bioresource Technology》;20111231;第102卷;摘要，第10672页左栏第3段-4段，右栏第1段 *
pectate lyase [Bacillus licheniformis];Berensmeier,S et al;《GenBank: CAD56882.1》;20050415;origin *
pectate lyase Pel-4B [Bacillus sp. P-4-N];Hatada,Y;《GenBank: BAA96478.1》;20070116;origin *
pectate lyase[Thermotoga maritima MSB8];Nelson,k.e. et al;《GenBank:NP_228243.1》;20121221;origin *

Also Published As

Publication number Publication date
CN103981166A (en) 2014-08-13

Similar Documents

Publication Publication Date Title
CN103981167B (en) The alkaline pectin enzyme mutant that a kind of heat stability improves
Yatmaz et al. Controlling filamentous fungi morphology with microparticles to enhanced β-mannanase production
Liu et al. A novel raw starch digesting α-amylase from a newly isolated Bacillus sp. YX-1: purification and characterization
Pirota et al. Enhancing xylanases production by a new Amazon Forest strain of Aspergillus oryzae using solid-state fermentation under controlled operation conditions
Li et al. Efficient simultaneous saccharification and fermentation of inulin to 2, 3-butanediol by thermophilic Bacillus licheniformis ATCC 14580
CN105143447B (en) Protein and application thereof with xylose isomerase activity
Lo et al. Characterization and high-level production of xylanase from an indigenous cellulolytic bacterium Acinetobacter junii F6-02 from southern Taiwan soil
Bader et al. Efficient saccharification of microalgal biomass by Trichoderma harzianum enzymes for the production of ethanol
CN102787130A (en) Acid and high temperature resistant alpha-amylase, and its gene, engineering bacterium and preparation method
CN102206659A (en) Method for enhancing activity of endoglucanase on basis of error-prone PCR (Sequential Error-Prone) technology
CN103642778A (en) Beta-endoglucanase mutant and application thereof
CN110272858A (en) A kind of bacillus coagulans of high yield L-lactic acid and its application
CN103966195B (en) A kind of than the enzyme alkaline pectin enzyme mutant improving alive
CN105886484A (en) Thermophilic cellulase, encoding gene thereof and application of thermophilic cellulase
CN105316310A (en) Alkaline pectinase mutant with improved specific enzyme activity and heat stability
CN105039371A (en) Trehalose synthase-trehalose hydrolase fusion enzyme, expression gene thereof and application
CN101307296A (en) Pichia yeast engineering strain for expressing Thermomyces lanuginosus gene 1n
CN105039374A (en) Starch induction type recombinant bacillus subtilis as well as preparation method and application thereof
CN103966194B (en) The alkaline pectin enzyme mutant that a kind of secernment property improves
CN102559567A (en) Construction of thermophilic endo-xylanase gene engineering strain and application of endo-xylanase of strain
CN103981166B (en) The alkaline pectin enzyme mutant that a kind of thermotolerance improves
CN103614354B (en) A kind of saccharifying enzyme and recombinant strains thereof
CN102994476B (en) Saccharifying enzyme
CN103045624B (en) High-temperature resistant and high-alkali resistant xylanase as well as gene, engineering bacterium and preparation method of xylanase
CN104531732A (en) Optimized extremely-thermostable xylanase XYNH coding gene and application thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20160413

Termination date: 20170507