CN103977122A - Application of effective part of longan pulp in preparing medicament related to anti-aging mechanism - Google Patents

Abstract

The invention discloses application of the effective part of traditional Chinese medicament longan pulp in preparing medicaments related to anti-aging mechanism. The water part of in-vitro longan pulp has a remarkable protecting effect to toxicity of a PC12 cell caused by A beta 25-35, and the in-vivo and in-vitro longan pulp water part has an effect of remarkably improving dysmnesia of an Alzheimers disease model mouse SAMP8 and increasing the content of SOD (superoxide dismutase) and GSH-PX (glutathione peroxidase) to play a role in resisting oxidation.

Description

The application of Arillus Longan effective site in the preparation medicine relevant to mechanism of resisting senility

Technical field

The present invention relates to a kind of field of traditional Chinese medicine pharmacy, be specifically related to the application of Chinese medicine Arillus Longan water position in the preparation medicine relevant to mechanism of resisting senility.

Background technology

Oxygen is the necessary element of human survival, if but redox equilibrium in body destroyed, unnecessary oxygen-derived free radicals can cause oxidative damage to health.More and more studies have shown that, this type of oxidative damage has been participated in the pathological process of the multiple neurological type diseases such as senile dementia directly.Source and the effective antioxidation approach of making oxidative stress damage clear, have very important significance to understanding the pathogenesis of the neurological type diseases such as senile dementia and the Therapeutic Method of Development of Novel.

Alzheimer disease (Alzheimer, Alzheimer's disease, AD), be the nervous system degenerative disease that a kind of reason is not bright, the cognitive decrease of take is main clinical manifestation, become one of disease that in world wide, harm humans erosion health is the most serious.Onset is slow, progress is slow, and the course of disease is 5～10 years, shows as clinically dysmnesia, aphasia, apraxia, agnosia, visual space function damage, abstract thinking and calculating damage, personality and behavior change etc.Along with the growth of elderly population, AD has become the commonly encountered diseases of modern society.Research discovery, in 75～85 years old old man, dull-witted sickness rate is similar to heart disease, is about 2.4%; The Baltimore city of the U.S. and studies show that of Sweden, within 60～65 years old, AD sickness rate is 1%.Other community studys show: the serious dull-witted prevalence 4.6% of over-65s, wherein mild to moderate cognitive dysfunction person surpasses 10%.In recent years epidemiologic data shows, China over-65s crowd's prevalence is about 5% at present, and 85 years old above prevalence is about 20%, and along with the increase of China's population life expectancy, its number of patients can increase gradually.Expect the year two thousand fifty, China AD patient will reach more than 2,500 ten thousand, then will become an important restriction factor that affects family and social development.Control AD has become focus and the difficult point of current world the world of medicine.For the treatment of AD, Chinese scholars has all been carried out a large amount of research work, and has obtained some new progresses.But the forming process more complicated due to AD, also relates to too many levels, a plurality of systems abnormal.So the treatment for AD also lacks more satisfactory medicine so far.Therefore, the pathogenesis of further investigated AD, the medicine of finding effectively prevention and treatment AD has important meaning.

Arillus Longan another name Arillus Longan, Fructus Alpiniae Oxyphyllae, Sapindaceae, Euphoria; Nature and flavor, sweet, flat temperature, nontoxic.Gui Jing, enters the heart, spleen, stomach.Arillus Longan has multiple efficacies such as having tonifying YANG QI invigorating, invigorating the heart and spleen, nourishing blood to tranquillize the mind.Have the effect of anti-senility, because it can suppress to make in human body the activity of a kind of enzyme of people's aging, add contained rich in protein vitamin and mineral, food can " make light of one's life by commiting suicide people not old " for a long time; Arillus Longan can also benefiting qi and nourishing blood, and neurasthenia, the hypophrenia are had to good curative effect, is the good merchantable brand of nourishing the brain and improving intelligence.Modern pharmacological research shows the model mice with the continuous gavage 30d AD of variable concentrations Arillus Longan, do diving tower experiment and Y maze experiment and find that treatment group ability of learning and memory significantly raises, Arillus Longan has adjusting peroxidase activity, prevention oxidative stress function, Arillus Longan extract not only can regulate endogenic oxidative stress damage in brain, and the oxidative stress of neural poison induction is also had to regulating action, can also affect the cascade of hippocampus amyloid, change the pathology of amyloid beta in PSAPP mouse brain, the response to oxidative stress that adjustment follows Alzheimer neural degeneration to change.

Summary of the invention

The invention discloses a kind of Arillus Longan water extractive part and application thereof.Arillus Longan obtains total extract with alcohol steep, is dissolved in water into suspension, centrifuging and taking supernatant, and concentrating under reduced pressure is dry, obtains water extractive part.By in body, external pharmacological evaluation all proves that water position has the pharmacological action of antioxidation and anti-senile dementia, can be used for the medicine of the anti-alzheimer disease medicine that preparation is relevant to Antioxidation Mechanism.

Concrete grammar and result confirm by following experiment:

One, the preparation at Arillus Longan water position

1 medical material Arillus Longan: Arillus Longan is purchased from Nanning Chinese crude drug distribution center, is accredited as the sarcocarp of Sapindaceae Euphoria plant Arillus Longan (Dimocarpus longan Lour.) through professor Wei Songji of Guangxi traditional Chinese medicine university.

2 extract with separated: dry Arillus Longan is made to coarse powder, by 95%, 70%, 50% alcohol dipping, extract 3 times respectively, each 4～5d that soaks, merge extractive liquid,, decompression recycling ethanol, obtains total ethanol extract, water is dissolved into suspension again, centrifuging and taking supernatant again, concentrating under reduced pressure is dry, i.e. get Shui position.

Two. remaining Arillus Longan water position drug efficacy study

(1) pharmacodynamic study of Arillus Longan water position to AD cell model

1 experiment material

1.1 tested medicines

Arillus Longan water position.

1.2 cell strain

Mice adrenal chromaffin tumor cell strain PC12, purchased from Shanghai cell biological institute cell bank.

1.3 instrument

CO ₂incubator (Thermo Forma), the U.S. produces, model: 381

Inverted fluorescence microscope (Nikon), Japan produces, model: TE2000-U

Inverted microscope (Olympus), Japan produces, model: CK40

Microplate reader (Thermo Forma), the U.S. produces, model: MULTISKANMK3

Ten thousand/gram electronic balance, Swiss, model: AG135

Culture bottle, U.S. Promega Corporation produces

96 orifice plates, corning, the U.S. produces

Micro sample adding appliance, Japan produces, model: Nichipet

Low speed desk centrifuge, Town in Shanghai booth instrument plant, model: TDL-5

Automatic high pressure steriliser, Japan produces, model: HV-50

Ice machine, Japan produces, model: SIM-F124

Superclean bench: Dutch Clean Air Techniek model: DLF560

Automatic dual pure water distillator, Shanghai Yarong Biochemical Instrument Plant, model: SZ-93

Accurate pH meter, Anping, Shanghai Lei Ci instrument plant, model: PHS-3C

The multiplex agitator of speed governing, Changzhou Guohua Electric Appliance Co., Ltd., model: HY-4

High-power magnetic stirring apparatus, Changzhou Guohua Electric Appliance Co., Ltd., model: 99-1

Filter, the U.S. produces, model: Millex-GP

A lowpressure aspirator, Medical suction unit factory of Shanghai Medical appliance industry (group) company, model: DY-1

Cryogenic refrigerator (Thermo Forma), the U.S. produces, model: 725

Constant Temp. Oven, Shanghai leap medical apparatus and instruments factory, model: 202-3

Electro-heating standing-temperature cultivator, Shanghai leap medical apparatus and instruments factory, model: 500-BS-11

1.4 reagent

MTT (tetramethyl tetrazolium bromide) is U.S. Amresco company product, lot number: 0793

FBS (embryo's Ox blood serum) is U.S. Hyclone company product, lot number: 070205

HBS (horse serum) is U.S. GIBCO company product, lot number: 201404

A β _25-35fragment is U.S. GIBCO company product, lot number: 050M4765

RPMI1640 culture medium is U.S. GIBCO company product

Trypsin (trypsin) is purchased from Xi Bao bio tech ltd, Shanghai, lot number: 900207D

DMSO (dimethyl sulfoxide) is purchased from Tianjin Hui Ying chemical reagent company limited, lot number: T20070605

HCl (hydrochloric acid) is purchased from Luoyang City's chemical reagent factory, lot number: 100412

Hoechst33258 is purchased from Kai Ji biotechnology Development Co., Ltd, lot number: 090104

Sodium bicarbonate is purchased from Bo Di chemical inc, Tianjin, lot number: 20101203

Sodium Pyruvate is purchased from Tianjin Kermel Chemical Reagent Co., Ltd., lot number: 20100110

L-glutaminate purchased from perseverance because of biology

The preparation of 1.5 main agents and medicine

1.5.1RPMI1640 culture medium: take 0.3g L-glutaminate, 0.1g Sodium Pyruvate, 2g sodium bicarbonate, a bag RPMI1640 dry powder, is dissolved in 1L distilled water, and adjusting PH is 7.2～7.4, filtering with microporous membrane subpackage, 4 ℃ of preservations.

1.5.2Trypsin liquid: be dissolved in distilled water, ultrasonic dissolution, the filtering with microporous membrane subpackage of 0.22 μ m ,-20 ℃ save backup.

1.5.3MTT liquid (5mg/mL): precision takes 100mg MTT, is dissolved in 20mL distilled water, ultrasonic dissolution, and the filtering with microporous membrane degerming of 0.22 μ m, 4 ℃ keep in Dark Place, in 2w, are finished.

1.5.4100 μ M (μ mol/L) A β _25-35solution preparation: get 500 μ gA β _25-35powder is dissolved in the dilution of 4.72mL aseptic double-distilled water, with after organic facies 0.22 μ m microporous filter membrane degerming subpackage, is placed in 37 ℃ of constant incubators and hatches 7d to reach " aging " A β _25-35object ,-30 ℃ of preservations.

1.5.5Hoechst33258 storage liquid: take Hoechst33258 powder 1mg, filter after 20mL distilled water dissolves, 4 ℃ keep in Dark Place.10 times of used time distilled water are diluted to working solution.

1.5.6 mounting liquid: 20nmol/L citric acid, 50nmol/L sodium hydrogen phosphate, volume fraction is 50% glycerol.

1.5.7 fixative: methanol mixes with glacial acetic acid volume ratio at 3: 1,4 ℃ save backup.

1.5.8 the preparation of culture fluid: RPMI1640+5%HBS+2.5%FBS

1.5.8 tested medicine

Arillus Longan water position: take 4.8mg water position, with a small amount of DMSO (final concentration that makes DMSO in medicine is 0.5%) and distilled water, be dissolved to 3mL, making drug level is 1.6mg/mL, then with aseptic double-distilled water dilution, making medicine final concentration is 80 μ g/mL, 40 μ g/mL, 20 μ g/mL, 10 μ g/mL, 5 μ g/mL.All samples is finally all used organic facies 0.22 μ m microporous filter membrane degerming subpackage, and 4 ℃ save backup.

2 experimental techniques

Adopt A β _25-35damage PC12 cell is set up AD cell model.Evaluate the curative effect of Arillus Longan water position to AD.First explore A β _25-35concentration, this is because A β _25-35concentration is too low can not produce the pathological change of reflection AD cell model, and concentration is higher, and cell is impaired seriously also can not correctly reflect AD model, also Estimating curative effect correctly just.Prerun according to bibliographical information in conjunction with us, we think A β _25-35concentration in culture fluid is advisable with 20 μ M.

2.1 preventive effect of Arillus Longan water position to AD cell model

2.1.1 cell culture (recover, cultivate, go down to posterity and frozen)

Frozenly in the adrenal chromaffin oncocyte PC12 of liquid nitrogen cell strain, be placed in immediately the water-bath of 37 ℃, make it to melt fast, the centrifugal 5min of room temperature 2000r/min, abandons supernatant, and cell dilution becomes 10 ⁵the cell suspension of individual/mL, puts into culture bottle, and 37 ℃, 10%CO ₂in incubator, cultivate, every other day change culture fluid once.The trophophase cell of taking the logarithm, abandons old culture fluid, adds 0.25% pancreatin to digest in right amount, the centrifugal 5min of 2000r/min, and piping and druming makes into individual cells repeatedly, is resuspended in containing in the cryopreserving liquid of 10%DMSO (10 ⁷individual/mL), in cryopreservation tube subpackage, slowly frozen.

2.1.2MTT method

The take the logarithm PC12 cell of trophophase, trypsinization, adjusting concentration is 3 * 10 ⁵the single cell suspension of individual/mL, is inoculated in 96 well culture plates, every hole 100 μ L, and after 24h, adding respectively the medicine culture fluid of variable concentrations and final concentration is the A β of 20 μ M _25-35solution, matched group adds the culture fluid (containing the DMSO of 0.5% final concentration) of equal-volume solvent, establish 4 multiple holes for every group, put in incubator and cultivate 72h, before stopping, every hole adds the freshly prepared serum-free medium containing 0.2mg/mLMTT of 200 μ L, continue to cultivate 4h, abandon supernatant, every hole adds 200 μ LDMSO, after vibration mixes, in microplate reader, take wavelength as 500nm measures OD value, calculate cell survival rate.

Computational methods: survival rate (%)=(experimental group cell viability/blank group cell viability) * 100%.

2.1.3 statistical procedures

With SPSS11.7 statistics software kit, carry out statistical procedures.Measurement data with represent.Between group, relatively adopt paired t-test.

2.2 therapeutical effect of Arillus Longan water position to AD cell model

2.2.1MTT method

The take the logarithm PC12 cell of trophophase, trypsinization, adjusting concentration is 3 * 10 ⁵the single cell suspension of individual/mL, is inoculated in 96 well culture plates, every hole 100 μ L, and after 24h, adding final concentration is the A β of 20 μ M _25-35solution, matched group adds the culture fluid (containing the DMSO of 0.5% final concentration) of equal-volume solvent, treats A β _25-35after effect 24h, adding respectively the medicine culture fluid of variable concentrations and final concentration is the A β of 20 μ M _25-35solution, matched group adds the culture fluid (containing the DMSO of 0.5% final concentration) of equal-volume solvent, establish 4 multiple holes for every group, put in incubator and cultivate 72h, before stopping, every hole adds the freshly prepared serum-free medium containing 0.2mg/mL MTT of 200 μ L, continue to cultivate 4h, abandon supernatant, every hole adds 200 μ L DMSO, after vibration mixes, in microplate reader, take wavelength as 500nm measures OD value, calculate cell survival rate.

Computational methods: survival rate (%)=(experimental group cell viability/blank group cell viability) * 100%.

3 experimental results

3.1 preventive effect of Arillus Longan water position to AD cell model

3.1.1MTT method

Without A β _25-35the PC12 cell OD value of solution with without medicine and containing 20 μ lA β _25-35the PC12 cell OD value of solution is compared has the difference of highly significant (P < 0.05), with without A β _25-35the survival rate of the PC12 cell of solution is as radix (100%), without medicine and containing 20 μ lA β _25-35the survival rate of the PC12 cell of solution is 75.93%, and apoptosis rate is 24.07%, and modeling success is described.

Experimental result is in Table 1.Arillus Longan water position within the scope of concentration 5～80 μ g/mL to containing 20 μ MA β _25-35the propagation of the PC12 cell of solution all has growth facilitation action in various degree (to contain 20 μ MA β _25-35solution, drug level is that the PC12 cell survival rate of 0 μ g/mL is radix), wherein 40～80 μ g/mL are concentration dependent, all the rising survival rate along with drug level increases gradually, compare with blank group and all have significant difference (P < 0.05), wherein 80 μ g/mL have significant differences (P < 0.05).

Table 1 variable concentrations water position acts on the OD value after AD prophylaxis model, survival rate (%)

Note: with not containing A β _25-35and the blank group of medicine compares, P < 0.05; With the A β containing 20 μ M _25-35solution and do not compare Δ P < 0.05 containing the blank group of medicine

3.1.2 Arillus Longan water position Contained Serum is to external A β _25-35the impact of damage PC12 cell proliferation

Arillus Longan water position Contained Serum is to external A β _25-35damage PC12 cells in vitro has the effect that promotes propagation, and 10% Arillus Longan water position Contained Serum and blank Contained Serum comparison, without significant (P < 0.05); 2.5% Arillus Longan water position Contained Serum is to external A β _25-35damage PC12 cell has increment trend, but compares with blank Contained Serum.Without significant.In Table 2.

Table 2 Arillus Longan water position 10% Contained Serum experiment in vitro

Note: 10% Contained Serum and the comparison of blank group, ^*p < 0.05

(2) the protective effect research of Arillus Longan water position to SAMP8 mice

1 experiment material

1.1 medicines and reagent

Arillus Longan water position, huperzine A (Hup-A, Zhejiang Zhenyuan Pharmaceutical Co., Ltd; 110402), glutathion-peroxide enzyme reagent kit (Mouse GSH-PX Elisa kit lot number:, R & D, 201112), superoxide dismutase test kit (Mouse SOD Elisa kit, R & D, lot number: 201112) lot number:

1.2 experiment equipment

Morris water maze: Beijing Chinese Academy of Sciences's physiology is produced (comprising Morris water maze, computer camera system, software kit analytical system), microplate reader, and (U.S. produces, model: MULTISKANMK3), centrifuge (Town in Shanghai booth instrument plant, model: TDL-5), vortex mixer XH-C (Jintan City Medical Instruments factory), high speed centrifuge (Germany, model: 1-14), palm-type centrifuge (Haimen City kylin medical apparatus factory, model: LX-200)

1.3 laboratory animal

Healthy quick aging Model of Dementia mice SAMP8 (hereinafter to be referred as mice) 64, body weight 20～30g.Laboratory animal provides (quality certification number: SCXX (Tianjin) 2008-0001) by Tianjin University Of Traditional Chinese Medicine.

2 experimental techniques

2.1 acute toxicity testing

Because being subject to the impact of concentration and volume, single administration cannot be measured its LD ₅₀therefore, measure its maximum tolerated dose.Get 20 of healthy mices, male and female half and half, body weight 18～22g.Before experiment, water 12h is can't help in animal fasting, then gives mouse stomach Arillus Longan water position 0.2mL/10g, and gavage 2 times, every 6h gavage 1 time, is then raised routinely, observes 14d.After administration, 7d, 14d respectively weigh once respectively.In 14d, put to death mice, dissect, perusal important organ (heart, liver, spleen, lung, kidney) has or not the pathologic of drug-induced to change.By weight of mice situation, calculate body weight gain rate.And calculate the maximum dosage-feeding of mice.

The 2.2 improvement effects of Arillus Longan water position to the behavior of SAMP8 mouse memory

2.2.1 animal grouping

Get 40 of SAMP8 mices, be divided at random 5 groups, be i.e. 8 of dosage groups, 8 of Arillus Longan water position low dose group in 8 of model control group, 8 of Hup-A groups, 8 of Arillus Longan water position high dose group, Arillus Longan water position.

2.2.2 administration

Administration after Animal adaptability nursing 2w.Arillus Longan water position, Hup-A group, with gastric infusion (ig) after DDW allotment.Arillus Longan water position: high dose 1g/mL (crude drug amount, lower same), middle dosage 0.5g/mL, low dosage 0.25g/mL; Hup-A organizes 0.038mg/kg; Model control group gives the DDW gavage (0.2mL/10g) of same volume.Every day, gavage was 1 time, successive administration 2 months.

2.2.3 mice learning and memory performances test

Adopt MWM method of testing, position navigation experiment and space exploration experiment.MWM makes with reference to related documents.Labyrinth is placed in the middle of room, is a circular rustless steel pond, diameter 200cm, and high 50cm, depth of water 30cm, water temperature remains on 26 ± 1 ℃.According to east (E), south (S), west (S), (N) orientation, north, pond is divided into southwest, the southeast, northwest, northeast (EN, quadrant 1) (WN, quadrant 2) (ES, quadrant 3) (WS, quadrant 4) four quadrants.At WN quadrant (quadrant 2) center, place a platform, platform is circular, diameter 11cm, the high 29cm of platform (be platform lower than water surface 1cm, claim to hide platform).Directly over labyrinth, 2m eminence is equipped with a miniature camera, and is connected with monitor with the videocorder in another room, records swimming track and the swimming time of mice.In pond, labyrinth, pour appropriate ink into, so that pond interior edema can be by all level dyeing is black.Room is airtight, keeps quite, and room door and window hides with light tight curtain, indoor with constant lighting, assurance brightness is impartial, pastes that to take different paintings be the object of reference that mice finds platform on indoor wall, and indoor placement one recorder is uninterruptedly play music noise as a setting with small volume.MWM test program is as follows:

(1) labyrinth adaptive training/Burden-Swimming Ability of KM is measured.MWM tests 1d, removes platform, allows Model of Dementia mice free swimming 2min in pond, and the swimming track of the lower tested mice of record calculates each Mus swimming distance.

(2) orientation navigation experiment (place navigation).Carry out altogether 5d.Be 2d to the 6d in MWM experiment, position sea trial.Platform is positioned at the 2nd quadrant, 1cm in underwater, mice place of entry is all quadrants pool wall mid point, every natural gift two time periods of morning and afternoon, every Mus training of each time period 4 times, mice is discharged water in pond by 4 place of entry towards pool wall, survey and in its 60s, successfully move into platform (mice is found platform and is detained 5s on it and successfully moves into) required time (being escape latency, escape latency).As mice in 60s can not successfully be moved into platform, experimenter is drawn upper mounting plate and makes it stop 10s, and recording escape latency is 60s.Mice place of entry every day is sequentially: 1d, WS-EN-WN-ES; 2d, ES-WN-EN-WS; 3d, WN-EN-WS-ES; 4d, WN-EN-ES-WS; 5d, WN-WS-ES-EN.

2.2.4 evaluation index and statistical method

Constant-bearing navigation experiment statistics amount is escape latency, seek number of times, the time of staying, platform quadrant percentage ratio.Result is analyzed with SPSS11.7for Windows statistical software.

The impact of 2.3 medicines on SAMP8 mice free radical metabolism

2.3.1 sample treatment

Mice behavior detects and finishes rear 1w, each is organized after mice execution, on ice platform, peel off rapidly the normal saline rinsing that mouse brain is organized in 4 ℃ of pre-coolings and remove blood, with filter paper, blot excessive moisture, on electronic balance, weigh, and add the ice normal saline of 9 times of volumes (weight/volume), and with high speed dispersion homogenizer, make 10% brain homogenate, get supernatant and respectively each group mouse brain tissue is carried out to each index determining by SOD, GSH-PX, test kit operating instruction.

2.3.2 index determining and statistical procedures:

Choose SOD, GSH-PX, as radical metabolism index of correlation, measure, with SPSS11.7for Windows statistical software, carry out statistical procedures.

3 experimental results

3.1 acute toxicity tests (MTD mensuration)

Continuous Observation 14d, the outward appearance of all animals, sign, behavioral activity, the mental status, diet, urine just etc. all do not have abnormal change.Mouth, eye, nose etc. are located without abnormal secretions, and all administration animals are without death.After 14d, de-neck is put to death, and dissects, and naked eyes are seen and looked into each main organs (heart, liver spleen, lung, kidney), and result shows that main organs position, color, size are all without abnormal.Arillus Longan water position Mouse Weight is female increases to 23.98 ± 3.43g by 17.70 original ± 1.44g; Malely by 25.67 original ± 4.67g, increase to 36.54 ± 3.87g, female mice body weight gain rate is 49.54%, and male mice body weight gain rate is 58.19%.As calculated, the maximum administration concentration 1g/mL of mouse stomach administration, is equivalent to 21 times of Coming-of-Age Day consumption, shows that toxicity is lower at Arillus Longan water position.

3.2 mice Burden-Swimming Ability of KMs are measured

The total distance of swimming that experiment starts labyrinth acclimatization training small mouse has represented the Burden-Swimming Ability of KM of mice to a certain extent.Each total distance of organizing mice 2min swimming is in Table 3.Experimental data shows that Hup-A group is higher than model control group, and low dose group, middle dosage group and high dose group are compared and are all greater than model control group with model control group respectively.

In labyrinth, the position acclimatization training of table 3 water position, respectively organize the move distance of mice

3.3 orientation navigation experiment

The statistics index of orientation navigation experiment is incubation period, seek number of times, the time of staying, platform quadrant percentage ratio, incubation period be that mice searches out platform for escaping water damage, and successfully move into the required time of platform.Can concentrated expression go out study, memory and the motor behavior ability of mice incubation period, and its value is less, illustrates that each ability of mice is stronger.Seek number of times and be that mice is found but the number of times that may not successfully move into platform, the variation of reflection mice learning and remembering ability, seeks number of times more, is to a certain degree to show that ability of learning and memory in mice is better.The time of staying be each time of mice through platform and resident total time on it, and seek number of times and combine and can rationally reflect the comprehensive variation of mice study, memory and behavior motor capacity.The time of staying is longer, and every integration capability of mice is stronger to a certain extent.

3.3.1 respectively organize the parameter 5d of mice MWM behavioristics average entire change seek number of times, the time of staying and platform quadrant percentage ratio 5d average entire change the results detailed in Table 4.

The table 4 water position relevant parameter 5d of Ge ZuMWM behavioristics average totally compares

Note: compare * P < 0.05 with model control group

Seek number of times: as can be seen from Table 4, high, the middle dosage group in Arillus Longan water position is sought number of times obviously more than model control group (P < 0.05), illustrates that it has improvement effect to dementia mice memory behavior.

The time of staying: as can be seen from Table 4, high, the middle dosage group time of staying of Arillus Longan water position, obviously more than model control group (P < 0.05), illustrates that it has improvement effect to dementia mice memory behavior.

In sum, the training of the memory behavior by 5d, the training achievement of Arillus Longan water position high, middle dosage group mice is compared obvious improvement with model control group, illustrate that thing Arillus Longan water of the present invention position optimal dose has the effect that improves senile dementia learning and memory behavior disorder.

3.4 Radical Metabolism indexs of correlation

3.4.1 Arillus Longan water position

Table 5 is each group free radical index of correlation Change in Mean, by table, can find out that model control group cerebral tissue SOD, GSH-PX activity respectively organize low compared with other.It is active that Arillus Longan water position and Hup-A medicine can improve SOD, GSH-PX.

Table 5 is respectively organized Radical Metabolism index of correlation Change in Mean

Note: each group is compared with model control group, * P < 0.05

(1) SOD:Hup-A group has been compared significant difference (P < 0.05) with model control group with high dose group, middle dosage group has been compared significant difference with model control group, and model control group and low dose group comparing difference not significantly (P > 0.05) between two.Referring to the known high dose group SOD level of table 5, apparently higher than other each groups, and middle dosage group and Hup-A group SOD is on close level.This shows that high dose group, middle dosage group and Hup-A medicine lowly all have protective effect to the SOD level due to quick aging Model of Dementia, can make SOD level raise, enhancing body oxidation resistance.

(2) GSH-PX: as shown in Table 5, Hup-A group relatively has significant difference (P < 0.05) with model control group, high dose group and model control group relatively have significant difference (P < 0.05), but low dose group and model control group comparing difference be remarkable (P > 0.05) not, and this also illustrates that Arillus Longan water position high dose group and Hup-A group are by making the level of GSH-PX raise enhancing body oxidation resistance.

In sum, antioxidation is played by raising antioxidase SOD, GSH-PX level in Arillus Longan water position, removes interior free yl, thus the effect of slow down aging.

The specific embodiment

Embodiment 1:

Take dry Arillus Longan coarse powder, by 95%, 70%, 50% alcohol dipping, extract three times successively, soak 4～5 days at every turn, merge extractive liquid,, decompression recycling ethanol, obtains total ethanol extract.Be dissolved in water into suspension, centrifuging and taking supernatant, concentrating under reduced pressure is dry, i.e. get Shui position.

Arillus Longan water position adds suitable drugs adjuvant, makes corresponding pharmaceutical dosage form, as capsule, tablet, granule, powder, oral liquid or pill, and the medicine that both must utilize thing of the present invention to make.

Claims (4)

1. an Arillus Longan effective part extract, can prepare by the following method:

Arillus Longan obtains total extract with alcohol steep, is dissolved in water into suspension, centrifugal, gets supernatant, and concentrating under reduced pressure is dry, get Shui position.

2. Arillus Longan effective part extract according to claim 1, the preparation method characteristic of this extract is that the method comprises the following steps:

A. Arillus Longan cleans, and is ground into coarse powder;

B. Arillus Longan coarse powder is extracted three times by 95%, 70%, 50% alcohol dipping successively, soak 4～5 days at every turn, merge extractive liquid,, decompression recycling ethanol, obtains total ethanol extract;

C. by step b) the Arillus Longan total extract of gained, be dissolved in water into suspension, centrifuging and taking supernatant, concentrating under reduced pressure is dry, get Shui position.

3. the application of Arillus Longan effective part extract according to claim 1 in the medicine of the preparation anti-alzheimer disease relevant to Aging mechanism.

4. application according to claim 3, the dosage form that it is characterized in that wherein said medicine is capsule, tablet, granule, powder, oral liquid or pill.