CN103962573A - Method for preparing biocompatible gold nano particles - Google Patents
Method for preparing biocompatible gold nano particles Download PDFInfo
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- CN103962573A CN103962573A CN201410199467.3A CN201410199467A CN103962573A CN 103962573 A CN103962573 A CN 103962573A CN 201410199467 A CN201410199467 A CN 201410199467A CN 103962573 A CN103962573 A CN 103962573A
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Abstract
The invention discloses a method for preparing biocompatible gold nano particles. The method comprises the following steps that chloroauric acid stock solution is used, deionized water is added to the chloroauric acid stock solution for attenuation, and the mixture is heated for 15 minutes through ultrasound; Tris (buffer solution ingredient of DNA and protein molecules) is dissolved in water of proper quantity; sodium hydroxide is used for being dissolved in water of proper quantity, and the Tris solution and the sodium hydroxide solution are mixed according to a certain ratio; the mixed solution is added in the chloroauric acid solution after 15 minutes to be heated for 30-60 minutes through ultrasound, the stable gold nano particles are obtained, and the size of each particle is 15-47 nm. The method is good in biocompatibility and environment friendliness, simple in operation, and easy to realize, the preparing process can not affect biomoecules, and the method can be more easily applied to the biomedical field.
Description
Technical field
The present invention relates to the preparation method of field of nanometer technology, be specifically related to a kind of biocompatibility golden nanometer particle preparation method.
Background technology
Golden nanometer particle (goldnanoparticles in metal nano material, AuNPs) there is good biocompatibility, molecular recognition function, be considered to be large biological molecule (nucleic acid molecules-DNA) super sensitivity detection always, the optimum carrier that bio-sensing and nucleic acid molecules or drug molecule transport at cell interior orientation. method the most classical in numerous preparation methods is the natrium citricum reducing process of Turkevitch-Frens, Brust-Schiffrin phase transfer method and seed mediated growth method (Seed-growthmethod), this several method is the most easily to accomplish on golden nanometer particle surface modified biological molecule. but, in the synthetic or modification of these methods, there is certain toxicity. in order to reduce the toxicity to living organism in golden nanometer particle experiment, the golden nanometer particle of realizing environmentally friendly and bio-compatible type is synthetic, and can there is better application at biomedical sector, constantly research and development biocompatible " green " method of reducing of researchers closely during the last ten years.So-called green synthesis method is directly to utilize biomolecule or biocompatible molecules to prepare golden nanometer particle as reducing agent and stabilizing agent, their preparation process is similar with natrium citricum method of reducing. and the Au III of can reducing becomes Au0 and keeps the stability of golden nanometer particle in solution. and existing " green " reducing agent has purification thing, the shitosan etc. of liposome, natural plants at present, but reaction temperature must reach more than 70 DEG C.Practical operation complexity is higher to operating personnel's technical requirement.
Summary of the invention
The deficiency existing for prior art, the object of the present invention is to provide on a kind of basis of reducing in " green ", makes reaction condition gentleer, the biocompatibility golden nanometer particle preparation method that experimental implementation is simple.
For achieving the above object, the invention provides following technical scheme: a kind of biocompatibility golden nanometer particle preparation method, comprises the following steps:
(1) get gold chloride stoste and add in deionized water and dilute, utilize ultrasonic machine, Ultrasonic Heating 15 minutes, wherein the mass ratio of gold chloride stoste is that concentration is 1%, the concentration mass ratio after dilution is 0.01%;
(2) take trishydroxymethylaminomethane and NaOH soluble in water respectively, tris solution is mixed by a certain percentage with sodium hydroxide solution;
After (3) 15 minutes, the mixed liquor of trishydroxymethylaminomethane and NaOH is added rapidly in chlorauric acid solution, continue ultrasonic 30-60 minute.
The present invention is further set to, and the initial temperature that the ultrasonic machine in step (1) is set is: 50 DEG C or 45 DEG C.
The present invention is also further set to: in step (2), the concentration of trishydroxymethylaminomethane is: 10mM, initial p H=7.4, the initial concentration mass ratio of NaOH is: 1%, and the volume ratio of mixed solution is: trishydroxymethylaminomethane: NaOH=2:2 and 1:2.
The present invention is also further set to: in step (3), the temperature of heating chlorauric acid solution after 15 minutes is approximately 30 DEG C, and the temperature of having reacted rear solution reaches 50 DEG C or 45 DEG C.
Advantage of the present invention is: compared with prior art, the present invention program is more reasonable, on the basis of " green " reduction, make reaction condition gentleer, experimental implementation is simple, utilize first trishydroxymethylaminomethane reduction gold chloride to prepare golden nanometer particle. trishydroxymethylaminomethane, buffer solution composition and the titrimetric standard thing in modal biochemistry and molecular biology experiment, experiment can complete at relatively suitable temperature, do not need to add any stabilizing agent or surfactant, and can be by regulating the amount of trishydroxymethylaminomethane, reaction time obtains different size, stable golden nanometer particle colloidal solution.
Below in conjunction with Figure of description and specific embodiment, the invention will be further described.
Brief description of the drawings
Fig. 1 is that the trishydroxymethylaminomethane of the embodiment of the present invention is prepared golden nanometer particle process drawing;
Fig. 2 is the uv absorption spectra of embodiment of the present invention golden nanometer particle;
Fig. 3 is the ultraviolet spectrogram of Au and Au-DNA under embodiment of the present invention equivalent trishydroxymethylaminomethane reaction condition.
Detailed description of the invention
Participate in Fig. 1, Fig. 2 and Fig. 3, specific embodiments is as follows:
Trishydroxymethylaminomethane (Tris (Hydroxymethyl) aminomethane is called for short Tris) described in the present embodiment, gold chloride-chemical formula HAuCl4, NaOH-chemical formula is NaOH.
Embodiment 1
A kind of biocompatibility golden nanometer particle preparation method disclosed by the invention, comprises the following steps:
(1) be that the conical flask of 0.01% HAuCl4 solution is put into ultrasonic machine by filling 50ml mass fraction, setting ultrasonic machine initial heating temperature is 50 DEG C, Ultrasonic Heating 15 minutes,
(2) the Tris-NaOH mixed liquor of getting 4ml volume ratio 2:2 adds in HAuCl4 solution;
(3) observe liquid color and change, in 2 minutes, liquid becomes lavender, persistent oscillation, and solution colour is deepened gradually, graduates into red by pink, after ultrasonic 60 minutes, stops;
(4) reducing solution in step (2) is changed into the Tris-NaOH mixed liquor of 3ml volume ratio 1:2, under same reaction conditions, repeat experiment.
Fig. 1 represents that Tris prepares golden nanometer particle process; Fig. 2 has provided the uv-visible absorption spectroscopy image of two kinds of colloidal solution that make, and ultraviolet absorption peak, respectively at 524.5nm and 535nm, all, in golden nanometer particle plasma resonance band scope, proves solution of gold nanoparticles.The average grain diameter of two kinds of golden nanometer particles is respectively: 24.81nm and 44.31nm.
Embodiment 2
(1) be that the conical flask of 0.01% HAuCl4 solution is put into ultrasonic machine by filling 50ml mass fraction, design temperature is 45 DEG C, Ultrasonic Heating 15 minutes, and actual temperature is 28 DEG C.
(2) utilizing above-mentioned Tris solution preparation concentration is 1ng/ulDNA solution, gets the NaOH solution of 2mlTris-DNA solution mixing 2ml, joins in the chlorauric acid solution of Ultrasonic Heating after 15 minutes.
(3) continue Ultrasonic Heating 60 minutes;
As can be seen from Figure 3, DNA molecular, in golden nanometer particle preparation process, keeps self property (the UV absorption frequency band of nucleic acid is at 260nm) constant, simultaneously Success in Experiment synthesized stable golden nanometer particle.
The present invention is on the basis of " green " reduction, make reaction condition gentleer, environment is good, experimental implementation is simple, utilize first trishydroxymethylaminomethane reduction gold chloride to prepare golden nanometer particle. trishydroxymethylaminomethane, buffer solution composition and the titrimetric standard thing in modal biochemistry and molecular biology experiment, experiment can complete at relatively suitable temperature, do not need to add any stabilizing agent or surfactant, and can be by regulating the amount of trishydroxymethylaminomethane, reaction time obtains different size, stable golden nanometer particle colloidal solution.
Above-described embodiment is to specific descriptions of the present invention; only be used to further illustrate the present invention; can not be interpreted as limiting the scope of the present invention, the technician of this area according to the content of foregoing invention to the present invention make some nonessential improvement and adjust all fall into protection scope of the present invention within.
Claims (4)
1. a biocompatibility golden nanometer particle preparation method, is characterized in that, comprises the following steps:
(1) get gold chloride stoste and add in deionized water and dilute, utilize ultrasonic machine, Ultrasonic Heating 15 minutes, wherein the mass ratio of gold chloride stoste is that concentration is 1%, the concentration mass ratio after dilution is 0.01%;
(2) take trishydroxymethylaminomethane and NaOH soluble in water respectively, tris solution is mixed by a certain percentage with sodium hydroxide solution;
After (3) 15 minutes, the mixed liquor of trishydroxymethylaminomethane and NaOH is added rapidly in chlorauric acid solution, continue ultrasonic 30-60 minute.
2. a kind of biocompatibility golden nanometer particle preparation method according to claim 1, is characterized in that: the initial temperature that the ultrasonic machine in step (1) is set is: 50 DEG C or 45 DEG C.
3. a kind of biocompatibility golden nanometer particle preparation method according to claim 1 and 2, it is characterized in that: in step (2), the concentration of trishydroxymethylaminomethane is: 10mM, initial p H=7.4, the initial concentration mass ratio of NaOH is: 1%, and the volume ratio of mixed solution is: trishydroxymethylaminomethane: NaOH=2:2 and 1:2.
4. a kind of biocompatibility golden nanometer particle preparation method according to claim 3, is characterized in that: in step (3), the temperature of heating chlorauric acid solution after 15 minutes is approximately 30 DEG C, and the temperature of having reacted rear solution reaches 50 DEG C or 45 DEG C.
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| CN201410199467.3A CN103962573A (en) | 2014-05-13 | 2014-05-13 | Method for preparing biocompatible gold nano particles |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111847671A (en) * | 2020-08-14 | 2020-10-30 | 中国石油大学(北京) | Bionic pilus honeycomb superstructure and preparation method and application thereof |
| CN111975010A (en) * | 2019-05-21 | 2020-11-24 | 中国药科大学 | Preparation of gold nanoparticles based on D-arabinose reduction |
| CN112535886A (en) * | 2020-11-12 | 2021-03-23 | 杭州苏铂科技有限公司 | Method for removing CTAB in gold nanorod solution |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1577928A (en) * | 2003-07-29 | 2005-02-09 | 中国科学院大连化学物理研究所 | High electrocatalytic active fuel battery platinum-base noble metal catalyst and producing process thereof |
| CN1579618A (en) * | 2003-08-06 | 2005-02-16 | 中国科学院大连化学物理研究所 | Carrying-type metal catalyst and its preparation method |
| CN1919459A (en) * | 2006-08-25 | 2007-02-28 | 武汉理工大学 | Preparing process for metal granular catalyst modified by ionic conduction polymer |
-
2014
- 2014-05-13 CN CN201410199467.3A patent/CN103962573A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1577928A (en) * | 2003-07-29 | 2005-02-09 | 中国科学院大连化学物理研究所 | High electrocatalytic active fuel battery platinum-base noble metal catalyst and producing process thereof |
| CN1579618A (en) * | 2003-08-06 | 2005-02-16 | 中国科学院大连化学物理研究所 | Carrying-type metal catalyst and its preparation method |
| CN1919459A (en) * | 2006-08-25 | 2007-02-28 | 武汉理工大学 | Preparing process for metal granular catalyst modified by ionic conduction polymer |
Non-Patent Citations (1)
| Title |
|---|
| FENG CHEN ET AL.: "A biocompatible synthesis of gold nanoparticles by Tris(hydroxymethyl)aminomethane", 《NANOSCALE RESEARCH LETTERS》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111975010A (en) * | 2019-05-21 | 2020-11-24 | 中国药科大学 | Preparation of gold nanoparticles based on D-arabinose reduction |
| CN111847671A (en) * | 2020-08-14 | 2020-10-30 | 中国石油大学(北京) | Bionic pilus honeycomb superstructure and preparation method and application thereof |
| CN112535886A (en) * | 2020-11-12 | 2021-03-23 | 杭州苏铂科技有限公司 | Method for removing CTAB in gold nanorod solution |
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