CN103961744A - Method for making simvastatin drug-containing hydroxyapatite coating on surface of pure titanium - Google Patents
Method for making simvastatin drug-containing hydroxyapatite coating on surface of pure titanium Download PDFInfo
- Publication number
- CN103961744A CN103961744A CN201310043285.2A CN201310043285A CN103961744A CN 103961744 A CN103961744 A CN 103961744A CN 201310043285 A CN201310043285 A CN 201310043285A CN 103961744 A CN103961744 A CN 103961744A
- Authority
- CN
- China
- Prior art keywords
- pure titanium
- simvastatin
- preparation
- hydroxyapatite coating
- electrochemical deposition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a method for depositing a simvastatin drug-containing hydroxyapatite coating on the surface of pure titanium by utilizing an electrochemical deposition process. The method comprises the following steps: polishing the surface of a pure titanium implant, sand-blasting, acid-etching, and depositing by utilizing an electrochemical deposition station, a simple apparatus and cheap materials to make the simvastatin drug-containing hydroxyapatite coating on the surface of the implant. The method has the advantages of simple preparation process, simple apparatus, low making cost, high making efficiency and the like, and the obtained coating has good biocompatibility and osteogenic cell proliferation differentiation promotion ability. A platform for making the drug-containing coating on the surface of pure titanium is established.
Description
Technical field
The present invention relates to a kind of method of preparing pastille hydroxyapatite coating layer on pure titanium surface.Especially, relate to a kind of electrochemical deposition method, method of the hydroxyapatite coating layer that contains simvastatin medicine at pure titanium surface deposition used.
Background technology
Constantly perfect along with planting technology, increasing agomphosis patient selects to plant repairing and treating.But because agomphosis overlong time or some systemic diseases as osteoporosis etc. may cause bone quantity not sufficient or the sclerotin change of planting area, thereby affect plantation success rate and long-time stability.Existing many studies confirm that, the surface topography of implantation body and chemical composition are integrated important role for the bone of implantation body.Simvastatin is a kind of fat-soluble statins, can induce expression and the stimulation bone formation of the mRNA of BMP-2.And hydroxyapatite is a kind of widely used coating material, because itself and bone have similar chemical composition, there is good biological activity simultaneously, therefore there is energy directly and the potentiality of bone surface combination.Widely used implantation body clinically at present, the general SLA technology that adopts in surface, i.e. sandblast acid etching coarse surface, its clinical application effect is better, but for the poor patient of local bone situation, still has the situation that plantation is failed.Therefore how can pass through to change implant surface pattern or composition, thereby effectively improve the poor patient's of bone condition plantation success rate, become the important research direction of global implantology.
Summary of the invention
What the object of the present invention is to provide a kind of high-biocompatibility and bone active forms the method containing the hydroxyapatite coating layer of simvastatin medicine on pure titanium surface.
The object of the invention is to be achieved through the following technical solutions: a kind of method that contains the hydroxyapatite coating layer of simvastatin medicine in the surface preparation of pure titanium, it is characterized in that, comprise the following steps:
(1) by pure titanium implant roughness be the polishing respectively of 320 orders, 500 orders and 800 object sand paper; With green silicon carbide sand grains under 4MPa pressure to the titanium surface sand-blasting after polishing; Use respectively acetone, 75% ethanol and distilled water, ultrasonic cleaning 15 minutes, and 50 DEG C of oven dry in baking oven;
(2) sample after cleaning is put into containing the Fluohydric acid. of 0.11mol/L and the salpeter solution of 0.09mol/L, and soaking at room temperature 10 minutes is rear with distilled water supersonic vibration cleaning 15 minutes twice, and 50 DEG C of oven dry in baking oven; Put into containing the hydrochloric acid of 5.8mol/L and the sulfuric acid solution of 8.96mol/L, 80 DEG C are soaked 30 minutes again, and rear taking-up is cleaned 15 minutes twice with distilled water sonic oscillation, and in baking oven 50 DEG C dry 24 hours;
(3) configuration simvastatin solution: 42 milligrams of simvastatins are dissolved in 1 milliliter of 95% alcoholic solution, add 0.1mol/L sodium hydroxide, with 0.1mol/L hydrochloric acid solution adjusting pH value to 7.2, deionized water is settled to 10 milliliters.
(4) electrochemical deposition: using pure titanium implant after treatment as the work utmost point (negative electrode), get one section of platinum filament as anode.Electrolyte is the lime nitrate 0.6mmol/L that contains AG, the deionized water solution of Ammonium biphosphate 0.36mmol/L, thus ensure that calcium-phosphorus ratio is 1.67, then add the Chile saltpeter of 0.1mol/L to increase electrolytical electric conductivity, add simvastatin solution, making its final concentration is 10 again
-6mol/L.Deposition process built in 3.0 volts, 85 DEG C, deposits 30 minutes by computer controlled.
The present invention has following technique effect, instrumental characterizing demonstration, and pure titanium implant surface forms column crystal, contains simvastatin medicine in crystal.In vitro cell experiment confirmation, there is not toxic action to cell in the standby coating of this legal system, preosteoblast is had to the effect that promotes proliferation and differentiation simultaneously.The present invention is by the electrochemical deposition of single, in the short period of time, prepared the hydroxyapatite coating layer containing simvastatin, preparation process material requested apparatus is comparatively simple, cost is lower, has realized the requirement of the artificial material of the short osteogenesis of low cost, high efficiency, high-biocompatibility.
Detailed description of the invention
By to after pure titanium implant surface finish, sandblast acid etching, utilize electrochemical deposition station, with comparatively simple apparatus and comparatively cheap material, the hydroxyapatite coating layer that contains simvastatin medicine in the preparation of implant surface deposition.In view of other surface treatment modes are for adding medicine to have some limitations, therefore the present invention selects electrochemical deposition method, thereby sets up comparatively easy drug deposition platform.
Feature of the present invention is to process pure titanium implant with sandblast aciding, the hydroxyapatite coating layer that contains simvastatin medicine is prepared in implant surface by electrochemical process, thus the pastille coating production that finds a kind of easy operating of low-cost high-efficiency to complete.
Claims (3)
1. the method containing the hydroxyapatite coating layer of simvastatin medicine in the surface preparation of pure titanium, is characterized in that, comprises the following steps: (1) is to the sand paper polishing of pure titanium implant surface, the sandblast of carborundum sand grains, cleaning, drying.(2) carry out secondary acid etching, cleaning, drying with different acid.(3) by ethanol, sodium hydroxide and hydrochloric acid preparation simvastatin solution for standby.(4) use electrochemical deposition station, by the electrolyte with simvastatin, the hydroxyapatite coating layer that preparation contains simvastatin medicine.
2. preparation method according to claim 1, is characterized in that: pure titanium surface is used to sand paper polishing, use the sandblast of green silicon carbide sand grains, use the mixed acid of special proportioning to carry out the pure titanium of secondary acid etching surface.
3. preparation method according to claim 1, is characterized in that: use electrochemical deposition station, simvastatin solution is added in electrolyte, thereby preparation is containing medicine coating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310043285.2A CN103961744A (en) | 2013-01-30 | 2013-01-30 | Method for making simvastatin drug-containing hydroxyapatite coating on surface of pure titanium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310043285.2A CN103961744A (en) | 2013-01-30 | 2013-01-30 | Method for making simvastatin drug-containing hydroxyapatite coating on surface of pure titanium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103961744A true CN103961744A (en) | 2014-08-06 |
Family
ID=51232053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310043285.2A Pending CN103961744A (en) | 2013-01-30 | 2013-01-30 | Method for making simvastatin drug-containing hydroxyapatite coating on surface of pure titanium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103961744A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105624753A (en) * | 2015-12-24 | 2016-06-01 | 广州中国科学院先进技术研究所 | Technology for uniform deposition of HA (hydroxyapatite) coatings on medical porous titanium and titanium alloy |
| CN111394766A (en) * | 2020-04-08 | 2020-07-10 | 浙江大学医学院附属口腔医院 | Pure titanium implant with cobalt-doped coating and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070289876A1 (en) * | 2006-06-15 | 2007-12-20 | Benli Luan | Bioceramic coating of a metal-containing substrate |
| CN101156963A (en) * | 2007-11-08 | 2008-04-09 | 浙江大学 | Method for preparing similar bone bioactivity coatings medical material by galvano-chemistry method |
-
2013
- 2013-01-30 CN CN201310043285.2A patent/CN103961744A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070289876A1 (en) * | 2006-06-15 | 2007-12-20 | Benli Luan | Bioceramic coating of a metal-containing substrate |
| CN101156963A (en) * | 2007-11-08 | 2008-04-09 | 浙江大学 | Method for preparing similar bone bioactivity coatings medical material by galvano-chemistry method |
Non-Patent Citations (2)
| Title |
|---|
| 杨国利: ""纯钛种植体不同表面的构建及骨结合研究"", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
| 杨帆: ""钛表面含辛伐他汀药物涂层的制备及其对骨质疏松症大鼠种植体骨结合作用的研究"", 《中国博士学位论文全文数据库 医药卫生科技辑》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105624753A (en) * | 2015-12-24 | 2016-06-01 | 广州中国科学院先进技术研究所 | Technology for uniform deposition of HA (hydroxyapatite) coatings on medical porous titanium and titanium alloy |
| CN105624753B (en) * | 2015-12-24 | 2018-08-07 | 广州中国科学院先进技术研究所 | A kind of medical porous titanium or titanium alloy uniform deposition hydroxyapatite coating layer technique |
| CN111394766A (en) * | 2020-04-08 | 2020-07-10 | 浙江大学医学院附属口腔医院 | Pure titanium implant with cobalt-doped coating and preparation method thereof |
| CN111394766B (en) * | 2020-04-08 | 2021-05-04 | 浙江大学医学院附属口腔医院 | Pure titanium implant with cobalt-doped coating and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Cipriano et al. | Anodic growth and biomedical applications of TiO2 nanotubes | |
| CN101332130B (en) | Process for providing a topography to the surface of a dental implant | |
| CN104674321B (en) | A kind of preparation method and application of titanium or titanium alloy surface copper-bearing antibacterial bioceramic film | |
| Guo et al. | Old is gold: electrolyte aging influences the topography, chemistry, and bioactivity of anodized TiO2 nanopores | |
| CN101984144B (en) | Surface mount method of mineralized collagen gradient coating for medical titanium implant | |
| CN103643274B (en) | A kind of method and application thereof preparing graphene oxide layer by being electrodeposited in titanium surface | |
| CN102912357B (en) | Method for preparing micro-nanostructure on surface of titanium implant | |
| CN104674320A (en) | Preparation method and application of wear-resistant antibacterial bioactive ceramic film for titanium or titanium alloy surface | |
| CN101972490A (en) | Anti-degradation antibacterial biological coating, and preparation method and application thereof | |
| CN105597157A (en) | Coating capable of promoting vascularization and anti-infection bioactivity and preparing method and application thereof | |
| CN101575726A (en) | Method for preparing bioactive gradient film of fluor-hydroxyapatite | |
| CN108079381A (en) | A kind of preparation method of medical titanium alloy surface biological Piezoelectric anisotropy coating | |
| CN106267342A (en) | A kind of dentistry implant and preparation method thereof | |
| CN104593850A (en) | Method for preparing composite bioactive coating based on titanium surface hierarchical pore structure | |
| CN108581392A (en) | A kind of preparation method and application of biological medical degradable magnesium alloy surface thin crystal composite layer | |
| CN103961744A (en) | Method for making simvastatin drug-containing hydroxyapatite coating on surface of pure titanium | |
| CN101455859A (en) | Titanium alloy surface Ti-YSZ-FHA biological gradient active coatings and preparation method thereof | |
| CN101380487A (en) | Preparation method of hydroxylapatite / zircite composite coating using pulse electrochemical deposition | |
| CN1282489C (en) | Boneoid apatite bio-active gradient coated artificial joint material and preparing process thereof | |
| CN103409715B (en) | A kind of porous TiO2/SiO2The preparation method of composite coating | |
| CN102286764A (en) | Method for preparing periodical absorbable Hyaluronic Acid/acyl carrier protein (HA/ACP) composite coating on titanium implant surface | |
| CN105565819B (en) | A kind of method and its application for adjusting silicon nitride ceramics bioactivity and bacteriostasis property | |
| CN101161295A (en) | Method for modifying medical metallic grafting body surface collagen by galvano-chemistry method | |
| CN108103551B (en) | A kind of method of hydroxylapatite crystal in promotion differential arc oxidation film layer | |
| RU2448741C1 (en) | Method of forming nanostructured biocompatible coating on implants |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20140806 |