CN103961389A - Mahonia extract, and preparation method and application thereof - Google Patents
Mahonia extract, and preparation method and application thereof Download PDFInfo
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- CN103961389A CN103961389A CN201310034208.0A CN201310034208A CN103961389A CN 103961389 A CN103961389 A CN 103961389A CN 201310034208 A CN201310034208 A CN 201310034208A CN 103961389 A CN103961389 A CN 103961389A
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Abstract
The invention provides Mahonia extract which is obtained by extracting Mahonia with ethanol. The Mahonia extract has effects of oxidation resistance, pain easing, inflammation resistance and liver protection. In addition, the invention also provides a preparation method and application of the Mahonia extract. The Mahonia extract can be applied to the preparation of medicine with functions of oxidation resistance, pain easing, inflammation resistance and liver protection.
Description
Technical field
The present invention is about Mahonia fortunei (Lindl.) Fedde (Mahonia) extract and the application in health care thereof, particularly about a kind of Mahonia fortunei (Lindl.) Fedde that contains active component (Mahonia) extract and in the purposes of antioxidation, analgesia, anti-inflammatory and hepatoprotective.
Background technology
Compatriots' hepatic disease attack rate is very high, and chronic hepatopathy and liver cirrhosis are the 6th of the ten large causes of death, and annual death toll is more than 3,000 people.Cause the principal element of hepatic disease to have viral, Alcoholic and chemical three major types.Wherein chemical liver injury in carbon tetrachloride enters body after, Cytochrome P450 in liver microsomes (cytochrome P450) participates in carbon tetrachloride (CCl
4) metabolism, form chloroform free radical (Trichloromethyl Free Radical, CCl
3), chloroform free radical can with oxygen effect, produce active larger and shorter peroxidating chloroform free radical (Trichloromethylperoxyl Radical, CCl of half-life
3oO).Peroxidating chloroform free radical can impel the sweet fabk polypeptide of bran Guang (Glutathione Reductase, GRD), sweet peptide peroxidase (the Glutathione Peroxidase of bran Guang, GPX), superoxide dismutase (Superoxide Dismutase, and catalase (Catalase SOD), the antioxidative enzyme activity decreased such as CAT), so that antioxidant with minimizing, thereby generation lipid peroxide, make AST(Aspartate Aminotransferase in liver) and ALT(Alanine Aminotransferase) ferment be released in blood plasma, cause the activity of AST and ALT in blood plasma to rise, finally cause hepatic fibrosis, hepatic necrosis and cause hepatic injury.In addition, carbon tetrachloride also can promote the formation of endogenous active oxygen and active nitrogen molecule, and it plays the part of very important role in hepatotoxic mechanism of causing a disease.In addition, in this process, the Kupffer cell of activation can discharge inflammatory factor, and it also can promote the hepatic injury of being brought out by carbon tetrachloride.
Mahonia fortunei (Lindl.) Fedde Wei little Tiller section (Berberidaceae) evergreen shrubs plant, its dried leaves, fruit, stem and root all can be used as medicine, and have effect of dampness, pathogenic fire purging, removing toxic substances dysentery relieving, can be used for improving pulmonary tuberculosis hemoptysis, osteopyrexia and fever, have a dizzy spell, hypertension.Modern pharmacological research more proves that it has the coronary artery blood flow of increasing, cardiotonic, contraception, antifertility and strengthens the pharmacological actions such as immunity.Although Mahonia fortunei (Lindl.) Fedde is in known effect that has multiple beneficial among the people, but it is not clear that its effective ingredient still belongs to.In addition, the physiologically active of Mahonia fortunei (Lindl.) Fedde is exploitation completely not, and it still has unknown curative effect, therefore have research Mahonia fortunei (Lindl.) Fedde in necessity of other physiology purposes.Therefore, there is the Mahonia fortunei (Lindl.) Fedde extract of how unknown curative effect (as: effect such as hepatoprotective, antioxidation) if can develop, for health care industry, can say very urgent and very important thing.
This case inventor is through verification experimental verification, it is unexpected that discovery, through the Mahonia fortunei (Lindl.) Fedde extract of extraction, have effect of multiple the unknown, as: antioxidation, analgesia, anti-inflammatory and hepatoprotective etc., except can, for for a long time by the bitter patient of hepatopathy provides a good recipe, also can be used as daily health care used.
Summary of the invention
An object of the present invention is to provide a kind of Mahonia fortunei (Lindl.) Fedde extract, and it has the physiologically active of multiple beneficial.
Another object of the present invention is to provide this Mahonia fortunei (Lindl.) Fedde extract, its purposes on antioxidation, analgesia, anti-inflammatory and hepatoprotective.
Another object of the present invention is to provide the method for this Mahonia fortunei (Lindl.) Fedde extract of preparation.
A further object of the present invention is to provide this Mahonia fortunei (Lindl.) Fedde extract, for the preparation of medical purposes.
The method that can reach foregoing invention object, includes: a kind of Mahonia fortunei (Lindl.) Fedde extract, its method by the following step is to make: with alcohol extraction Mahonia fortunei (Lindl.) Fedde, to make Mahonia fortunei (Lindl.) Fedde ethanolic extract, and after ethanol is removed, can make.Find through test of the present invention, this Mahonia fortunei (Lindl.) Fedde extract has effect of antioxidation, anti-inflammatory, analgesia and hepatoprotective.Therefore, Mahonia fortunei (Lindl.) Fedde extract of the present invention can be used for preparing the purposes pharmaceutically of antioxidation, anti-inflammatory, analgesia and hepatoprotective.These purposes comprise the form that this extract is prepared into various compositions, comprise the wieldy forms of daily life such as food, beverage, health food, additive, medical composition, for general user or sufferer, can be easy to long-term taking, to reach the effect of daily health caring or state of an illness control.
The present invention can, by the diagram after the description of following preferred embodiment and collocation, be able to more distinct; Even if wherein may change or modify, but neither deviate from the disclosed spirit of the present invention and category.
Brief description of the drawings
Fig. 1 is the writhing test result of Mahonia fortunei (Lindl.) Fedde extract.
Fig. 2 is that the formalin of Mahonia fortunei (Lindl.) Fedde extract is licked sufficient result of the test.
Fig. 3 A to C is that Mahonia fortunei (Lindl.) Fedde extract is in the section interpretation of result of tetrachloro-methane induction hepatic injury.The liver section that wherein Fig. 3 A is normal mouse, the liver section that Fig. 3 B is tetrachloro-methane induction group and Fig. 3 C are through tetrachloro-methane induction, and the liver section of feeding Mahonia fortunei (Lindl.) Fedde extract of the present invention.
Detailed description of the invention
All technical and scientific terminology described in the present invention, unless definition to some extent is in addition all this affiliated field and has the meaning that common skill person can understand jointly.
Singulative " one " and " being somebody's turn to do " of in the present invention, using comprise plural form, unless separately there is the clear person of indicating in literary composition.Therefore, for example, in the time mentioning " sample ", comprise a plurality of these equal sampleses and this field is had to the coordinate known to common skill person.
" approximately ", " approximately " or " being close to " second word using herein represents that in fact described numerical value or scope are positioned in 20%, be preferably in 10%, and better person are in 5%.Be approximation in digitized amount provided in this article, if also can be pushed away when intention term " about ", " approximately " or " being close to " are used.
The present invention is surprised to find that a kind of Mahonia fortunei (Lindl.) Fedde extract, and it has the biological activity of multiple beneficial, as: effect of antioxidation, anti-inflammatory, analgesia and hepatoprotective.The following row method of this Mahonia fortunei (Lindl.) Fedde extraction system is prepared and obtain: with alcohol extraction Mahonia fortunei (Lindl.) Fedde, to make Mahonia fortunei (Lindl.) Fedde ethanolic extract, after then ethanol being removed, can make Mahonia fortunei (Lindl.) Fedde extract.
In an embodiment, this Mahonia fortunei (Lindl.) Fedde is Alishan Mahonia fortunei (Lindl.) Fedde (Mahonia oiwakensis) or claims Yushan Mahonia fortunei (Lindl.) Fedde, emerald green peak Mahonia fortunei (Lindl.) Fedde.
In an embodiment, the concentration of this ethanol is approximately between 30% to 95%(percent by volume) between, preferably is 50%.
In an embodiment, this Mahonia fortunei (Lindl.) Fedde extraction position means root, stem, leaf portion, and preferably is stem and root.
In an embodiment, the step that ethanol is removed can be used concentrating under reduced pressure mode to remove it, or adopts this field to have known other usability methods of common skill person to remove ethanol.
In an embodiment, this Mahonia fortunei (Lindl.) Fedde extract confirms to have effect of antioxidation, anti-inflammatory, analgesia and hepatoprotective through test, can be used for preparing medical purposes.These medicine can be various wieldy forms, as: oral, inject, smear etc.In order to reach various wieldy forms, by applicable pharmaceutically acceptable excipient or the supporting agent of optionally arranging in pairs or groups.This pharmaceutically acceptable excipient can be and is suitable for the pharmaceutically acceptable organic or inorganic carrier mass that intestinal is outer, enteral is used, and this excipient can not produce harmful reacting with active extract of the present invention.Applicable excipient is including but not limited to water, saline solution, vegetable oil, Polyethylene Glycol, gelatin, amylose, lactose, magnesium stearate, Talcum, silicic acid, stickiness paraffin, fatty acid monoglyceride and glycerol, fatty acid ester, hydroxy methocel, polyvinylpyrrolidone etc.This pharmaceutically acceptable supporting agent comprises one or more and is selected from following reagent: solvent (solvent), emulsifying agent (emulsifier), suspending agent (suspending agent), distintegrant (decomposer), adhesive (bindingagent), tranquilizer (stabilizing agent), chelating agen (chelating agent), diluent (diluent), gellant (gelling agent), antiseptic (preservative), lubricant (lubricant), interfacial agent (surfactant), adjuvant (adjuvant), and other similar or applicable supporting agents of the present invention.
Term " protection, antagonism " means; compared to the animal or the cell sample that do not use Mahonia fortunei (Lindl.) Fedde extract of the present invention; use animal or the cell sample of Mahonia fortunei (Lindl.) Fedde extract of the present invention, there is higher oxidation resistance, anti-inflammatory ability, the pain sensation releive ability and liver-protective ability.
The present invention further sets forth it by following instance, and it is only for the object of example but not in order to limit to the present invention.
The preparation of embodiment mono-Mahonia fortunei (Lindl.) Fedde extract
Alishan Mahonia fortunei (Lindl.) Fedde (Mahonia oiwakensis) gathers from Alishan, Taiwan.After the root of Alishan Mahonia fortunei (Lindl.) Fedde, stem, leaf are cut into small pieces respectively, are placed in drying baker and dry, to be dried after, be milled to respectively powder.50% ethanol of 10 liters is added respectively in the powder of this root, stem, leaf, extract respectively 48 hours.Extract is filtered, filtrate is carried out to concentrating under reduced pressure in 40 ° of C, can make Mahonia fortunei (Lindl.) Fedde extract (root extract (MOR), stem's extract (MOS), leaf portion extract (MOL)).Concentrating under reduced pressure step has wherein been that ethanol is removed, other known applicable patterns also applicable it.
The oxidation resistance analysis (DPPH) of embodiment bis-Mahonia fortunei (Lindl.) Fedde extracts
Root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) are carried out respectively to the test of DPPH radical scavenging activity, to assess its oxidation resistance.DPPH test method, be summarized as follows: DPPH free radical is a kind of stable free radical, if the sample adding can be removed, represent that sample has the ability that reduces hydroxy radical, alkane free radical or peroxylradicals and the chain reflex effect of ending lipid peroxidation.DPPH methanol solution is darkviolet, has strong absorption at 517nm, adds after sample and measures with point luminometer under wavelength 517nm, can measure its removing effect to DPPH.Sample preparation is become to the solution (2000,1000,500 of variable concentrations, 250,125and62.5 μ g/ml), in 96 holes trace dishes, add the solution of the sample of 25 μ l variable concentrations, add immediately 175 μ l0.3mM DPPH methanol solutions, making last volume is 200 μ l.Vibration evenly mixed after 15 seconds, and under room temperature, lucifuge leaves standstill 30 minutes, under wavelength 517nm, with its light absorption value of spectrophotometric determination.In the time that DPPH free radical is eliminated the more, its light absorption value can decline the more, utilizes with respect to the light absorption value decline percentage ratio of blank group, power that can judgement sample scavenging ability of DPPH free radical, that is represent the power of sample hydrogen supply capacity.Radical scavenging activity computing formula is as follows: free radical scavenging percentage ratio (%)=[1-(ABS
517sample/ABS
517control group)] × 100%.
Result as shown in Table 1, with vitamin C (Vit.C) and BHT antioxidant (2,6-Di-tert-butyl-4-methylphenol) similar, stem's extract (MOS) and root extract (MOR) tool DPPH radical scavenging activity, the oxidation resistance of confirmation Mahonia fortunei (Lindl.) Fedde extract.
The DPPH radical scavenging activity of table one Mahonia fortunei (Lindl.) Fedde extract
The analgesia efficiency analysis of embodiment tri-Mahonia fortunei (Lindl.) Fedde extracts
Root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) are carried out respectively to writhing response test and formalin and lick sufficient test, to assess its analgesia effect.
Writhing response test method, be summarized as follows: by root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) and positive control group indomethacin (indomethacin), (20mg/kg) (non-steroid anti-inflammatory analgesic) is oral gives mice, after administration 60 minutes by lumbar injection 1% acetum to bring out writhing response (every 10g body weight casts 0.1ml), mice is placed in to inspection box, after 5 minutes, start to observe, and in 5-15 minute after Number device note Recording injection acetic acid, mouse web portion is because of the caused writhing of pain time Number.
Formalin is licked sufficient test method, be summarized as follows: give mice by (20mg/kg) oral to root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) and positive control group indomethacin (indomethacin), after administration 60 minutes, with No. 27 syringe needles of microsyringe, at the right back sufficient sole of the foot of mice, 5% formalin solution of subcutaneous injection 20 μ l, immediately mice is inserted in inspection box, observe mice and lick its right back sufficient sole of the foot injection of formalin position, calculate and lick the number of seconds (pain sensation response time) that the sole of the foot is accumulated.
Refer to Fig. 1, writhing test result shows, root extract (MOR) and stem's extract (MOS) are (Indo) similar to indomethacin (indomethacin), all can significantly reduce writhing response number of times, confirm analgesia effect of these extracts.In addition, refer to Fig. 2, formalin is licked foot test and is shown, identical, root extract (MOR) and stem's extract (MOS), similar to indomethacin (indomethacin), all can significantly reduce the sole of the foot time of licking (pain sensation response time), confirm analgesia effect of these extracts.
The anti-inflammatory analysis of embodiment tetra-Mahonia fortunei (Lindl.) Fedde extracts
Root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) are carried out respectively to the test of λ-carrageenin induction pedal swelling, to assess its anti-inflammatory effect.
λ-carrageenin induction pedal swelling test method, be summarized as follows: taking λ-carrageenin inducing mouse pedal swelling as experiment model, around mice right hind ankle joint, do a labelling with marking pen, the right back mice sufficient sole of the foot is immersed to edema determining instrument, after measurement normal foot sole of the foot volume, induce inflammation at the right back sufficient plantar subcutaneous injection 1% λ-carrageenin suspension of mice (50 μ l/ only) respectively.Induce after 2 hours, mice to give root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) and positive control group indomethacin (indomethacin) (20mg/kg), is offerd medicine with oral way respectively.Inject after 1% λ-carrageenin suspension induction inflammation, measured once sufficient sole of the foot volume every 1 hour, after discipline Recording result is also calculated respectively induction inflammation, (△ V) ， Even is continuous measures 5 hours to sufficient sole of the foot volume per hour, relatively its pedal swelling degree with the front indivedual sufficient sole of the foot volume differences of induction.
Refer to table two, result shows, root extract (MOR) and stem's extract (MOS) are similar to indomethacin (indomethacin), all can significantly reduce pedal swelling degree, confirm anti-inflammatory effect of these extracts.
The anti-inflammatory capability analysis of table two Mahonia fortunei (Lindl.) Fedde extract
The liver-protecting function analysis of embodiment five Mahonia fortunei (Lindl.) Fedde extracts
By root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) respectively with CCl
4the hepatic injury pattern of bringing out, assesses its hepatoprotective effect.By Herba Silybi mariani (silymarin), (200mg/kg) (positive controls, a kind of hepatoprotective) and root extract (MOR), stem's extract (MOS), leaf portion extract (MOL) (500mg/kg) give mouse three days continuously with oral way respectively.Afterwards, in lumbar injection mode by CCl
4(1ml/kg, 50%v/v) injects each group.After 24 hours, gather mouse blood to carry out the analysis of biochemical index (GOT and GPT).
Result demonstration, refers to table three, and GOT and GPT are usually used in detecting hepatocyte injury, once hepatocyte is impaired or downright bad, GOT and GPT just can enter in blood, and therefore numerical value can raise.Root extract (MOR) and stem's extract (MOS) are similar to positive controls (Herba Silybi mariani) significantly, can obviously reduce GOT that the hepatic injury of inducing because of carbon tetrachloride causes and the rising of GPT.Wherein better with stem's extract (MOS) effect again.
The GOT of table three Mahonia fortunei (Lindl.) Fedde extract and GPT analyze
In addition, refer to Fig. 3 A to C, be respectively the liver section of normal mouse, tetrachloro-methane induction group and stem's extract (MOS) group, can obviously find out that stem's extract (MOS) group can reduce the hepatic injury (vacuolation, inflammation and necrocytosis) of tetrachloro-methane induction.In addition,, on pathology index analysis, also can find out that stem and root extract can effectively reduce hepatic injury pathology index (vacuolation, inflammation and necrocytosis) (table four).Therefore, confirm that Mahonia fortunei (Lindl.) Fedde extract of the present invention has effect of hepatoprotective.
The analysis of table four pathology index
In sum, Mahonia fortunei (Lindl.) Fedde extract provided by the present invention has the biological activity of multiple beneficial, the effect that comprises antioxidation, analgesia, anti-inflammatory and hepatoprotective.
Above-listed detailed description system illustrates for a possible embodiments of the present invention, and only this embodiment is not in order to limit the scope of the claims of the present invention, does not allly depart from the equivalence that skill spirit of the present invention does and implements or change, and all should be contained in the scope of the claims of this case.
Claims (10)
1. Mahonia fortunei (Lindl.) Fedde (Mahonia) extract, obtained through the method for the following step: with alcohol extraction Mahonia fortunei (Lindl.) Fedde, to make Mahonia fortunei (Lindl.) Fedde ethanolic extract, after ethanol is removed, can to make Mahonia fortunei (Lindl.) Fedde extract.
2. extract as claimed in claim 1, wherein this Mahonia fortunei (Lindl.) Fedde is Alishan Mahonia fortunei (Lindl.) Fedde (Mahonia oiwakensis).
3. extract as claimed in claim 1, wherein this ethanol is between 30 to 95% concentration of volume percent.
4. extract as claimed in claim 1, wherein this Mahonia fortunei (Lindl.) Fedde means root and the stem of Mahonia fortunei (Lindl.) Fedde.
5. the extract as described in claim 1, wherein this Mahonia fortunei (Lindl.) Fedde extract has effect of antioxidation, anti-inflammatory, analgesia and hepatoprotective.
6. a preparation method for Mahonia fortunei (Lindl.) Fedde (Mahonia) extract, comprises the following step:
With alcohol extraction Mahonia fortunei (Lindl.) Fedde, to make Mahonia fortunei (Lindl.) Fedde ethanolic extract, after ethanol is removed, can make Mahonia fortunei (Lindl.) Fedde extract.
7. extract as claimed in claim 1, wherein this Mahonia fortunei (Lindl.) Fedde is Alishan Mahonia fortunei (Lindl.) Fedde (Mahonia oiwakensis).
8. extract as claimed in claim 1, wherein this ethanol is 30 to 95% concentration of volume percent.
9. extract as claimed in claim 1, wherein this Mahonia fortunei (Lindl.) Fedde means root and the stem of Mahonia fortunei (Lindl.) Fedde.
10. a Mahonia fortunei (Lindl.) Fedde as claimed in claim 1 (Mahonia) extract is in the medical purposes of preparing antioxidation, anti-inflammatory, analgesia and hepatoprotective.
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| CN201310034208.0A CN103961389A (en) | 2013-01-29 | 2013-01-29 | Mahonia extract, and preparation method and application thereof |
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| CN201310034208.0A CN103961389A (en) | 2013-01-29 | 2013-01-29 | Mahonia extract, and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265822A (en) * | 2016-10-10 | 2017-01-04 | 广东省中医院 | There are anti-inflammation raw myofunctional Mahonia bealei (Fort.) Carr. extract, preparation method and applications |
-
2013
- 2013-01-29 CN CN201310034208.0A patent/CN103961389A/en active Pending
Non-Patent Citations (2)
| Title |
|---|
| JUNG CHAO等: "Hepatoprotective Effect of Shidagonglao on Acute Liver Injury Induced by Carbon Tetrachloride", 《THE AMERICAN JOURNAL OF CHINESE MEDICINE》 * |
| 董雷等: "十大功劳属植物的药理作用研究进展", 《中国现代药物应用》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106265822A (en) * | 2016-10-10 | 2017-01-04 | 广东省中医院 | There are anti-inflammation raw myofunctional Mahonia bealei (Fort.) Carr. extract, preparation method and applications |
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Application publication date: 20140806 |