CN103960457A - Preparation method of high-solubility low-phytic-acid soybean protein isolate - Google Patents
Preparation method of high-solubility low-phytic-acid soybean protein isolate Download PDFInfo
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Abstract
A preparation method of a high-solubility low-phytic-acid soybean protein isolate belongs to the technical field of deep processing of soybeans. According to the preparation method, a soybean meal is firstly crushed to be good for the dissolution of the protein, then large particles like insoluble polysaccharide and fibers are screened to be removed, then a suspension liquid is prepared and then filtered and centrifuged after the protein is extracted through an alkaline process, the obtained protein solution is subjected to enzymolysis, proper optimization of the enzymolysis condition removes anti-nutritional factorphytic acid and keeps the solubleness of the protein, then acid precipitation and centrifugation are conducted, and the obtained milk curd is crushed, stirred, neutralized, sterilized and dried to obtain the soybean protein isolate. The preparation method is simple in operation and high in efficiency, the anti-nutritional factorphytic acid in the soybean protein isolate is effectively reduced, the protein is high in solubility under a neutral condition, and the preparation method is widely applied in the production like food processing, meat products, dairy processing.
Description
Technical field
The present invention relates to a kind of preparation method of the low phytic acid soybean protein isolate with high-dissolvability, be specifically related to the heavy soybean protein isolate that obtains low phytic acid with enzymatic treatment degraded phytic acid and there is high-dissolvability of the molten acid of a kind of combination alkali, belonged to soybean deep processing technology field.
Background technology
Soybean protein isolate is broad-spectrum vegetable protein raw material, it is the vegetable protein that can compare favourably with animal protein, the main method of domestic production soybean protein isolate is that the molten acid of alkali is heavy, raw material is mainly the defatted soybean meal extracting after soybean oil, in dregs of beans, contain higher phytic acid, content is up to 20.89mg/g, and in the soybean protein isolate of preparation, phytic acid content is also higher, and content range is at 13.65-19.87mg/g.Be applied in food processing and dairy products processing at soybean protein isolate, higher phytic acid content not only directly has influence on the dissolution characteristics of albumen, also can be due to the stronger sequestering power of phytic acid, be combined together to form insoluble compound with protein and mineral matter element, make soybean protein isolate be difficult to be absorbed and used, mineral matter element is also difficult to be utilized simultaneously.Phytase can impel phytic acid and phytate thereof to be hydrolyzed, produce Phos and inositol, therefore, by the phytase processing under temperate condition, can either significantly reduce phytic acid content, can make again gained soybean protein isolate under neutral environment condition, have high solubility, make its application in fields such as meat products, dairy products and wheaten food more extensive.
At present, the method that reduces phytic acid content has a lot, comprises the methods such as ion-exchange, ultrafiltration, interpolation phytase.For ion-exchange process, owing to using resin easily and protein combination, and resin is easily contaminated, applies limited in actual production.And for ultrafiltration, owing to will using milipore filter, protein macromolecule very easily stops up fenestra, separating effect is declined greatly, the yield of albumen declines, and gained albumen phytic acid content is still higher.Optimal pH for current industrial phytase used is sour environment, optimum temperature is between 55-65 DEG C, under this condition, carrying out enzymolysis can produce serious influence for producing soybean protein isolate, make albumen that sex change occur to assemble, long Temperature Treatment also can make other functional characteristics such as the solubility of albumen occur significantly to decline.Research shows, by utilizing the wider pH sphere of action of phytase of greater activity (200,000 U), even if also can be good at carrying out enzymolysis under the at room temperature shorter time, can reduce significantly phytic acid content, can ensure that again the soybean protein isolate making has the functional characteristics such as high dissolubility.
The problem such as the solubility that the present invention has well solved soybean protein isolate prepared by enzymolysis significantly reduces, phytic acid content is higher.A kind of soybean protein isolate of producing low phytic acid, high-dissolvability under gentleer environmental condition has easily been proposed.First by dregs of beans and water through the molten soy bean proteinous soln that makes of alkali, be further adjusted to peak enzymolysis-ability condition and carry out enzymolysis and remove ANFs phytic acid, the heavy centrifugal curdled milk that obtains of acid afterwards, further separates broken, neutralization and makes soybean protein isolate.
Summary of the invention
The solubility that the object of the invention is to overcome the soybean protein isolate that in prior art prepared by enzymolysis significantly reduces, the more high weak point of phytic acid content, proposes a kind of method of producing the soybean protein isolate of low phytic acid, high-dissolvability under gentleer environmental condition easily.
Technical scheme of the present invention: a kind of preparation method of the low phytic acid soybean protein isolate with high-dissolvability, dregs of beans is pulverized and sieved and removed after insoluble fibre impurity, alkaline process extracts and obtains water-soluble soybean protein solution, remove phytic acid through enzymatic hydrolysis, isoelectric precipitation obtains curdled milk, broken through separating, stir, neutralization, the dry soybean protein isolate that makes;
Concrete steps are as follows:
(1) pulverize and sieve: get pending dregs of beans, pulverize and sieve; Pulverization conditions is rotating speed 25000-35000rpm, and time 30s-2min pulverizes 2-3 time, every minor tick 30s, and sieve order number is 60-100 order;
(2) alkaline process extract soybean protein isolate: get step (1) pulverize after dregs of beans water-soluble, dregs of beans is 1:10-20 with water quality ratio, adding aqueous slkali to regulate pH is 7.0-7.5, at temperature 20-50 DEG C, reacts 1-2h, uses after completion of the reaction the gauze of layer 2-4 to filter; To filter gained mixture at the centrifugal 30min of 9500rpm, obtain protein solution stand-by;
(3) enzymolysis: get step (2) gained protein solution, add phytase to carry out enzymolysis, enzyme concentration is 15-200U/g dregs of beans, regulating enzymolysis pH with sour reagent is 4.5-6.5, and hydrolysis temperature is 10-30 DEG C, and enzymolysis time is 30min-2h;
(4) isoelectric precipitation: get product after step (3) enzymolysis and carry out isoelectric point precipitates albumen centrifugal, selected acid heavy with sour reagent adjusting pH be 4.4-4.6, temperature is room temperature, the heavy 30min-1h of acid, centrifugal condition is 5000-9500rpm, and time 15-30min, obtains curdled milk;
(5) post processing: the curdled milk to step (4) gained and deionized water are dissolved in the ratio of 1:10, separates broken and stirs at the high-speed shearing machine of 15000-30000rpm; Then add alkaline agent to neutralize, the finally dry 5-60s of spraying at 60-100 DEG C, obtains product and has the low phytic acid soybean protein isolate of high-dissolvability.
The described aqueous slkali of step (2) is that concentration is NaOH, potassium hydroxide or the aqua calcis of 2M.
The phytase adding in step (3) is: aspergillus niger or pichia yeast fermenting and producing.
Citric acid, hydrochloric acid or acetic acid that the adjusting pH value sour reagent used that step (3) and (4) are used is 2M for concentration.
The described pulverization conditions of step (1) is rotating speed 30000rpm, and the time is 30s, pulverizes twice, every minor tick 30s, and order number is 80 orders.
The mass ratio of the described dregs of beans of step (2) and water is 1:15, and extracting pH is 7.0, at 25 DEG C of temperature, reacts 1h, and the aqueous slkali of selecting is NaOH, potassium hydroxide or aqua calcis, and the gauze number of plies is two-layer.
The acid of step (4) heavy with salt acid for adjusting pH be 4.5, temperature is that room temperature, the acid time of sinking is 30min, centrifugal condition is 6000rpm, time 20min, obtains curdled milk.
Specific analytical method:
(1) phytic acid content assay method: with reference to GB/T 5009.153-2003 and A modified colorimetric method for phytic acid analysis in soybean;
Principle: phytic acid and the effect of ferric trichloride-sulfosalicylic acid mixed liquor, produce color fading reaction, phytic acid content is directly proportional to fading extent, measures absorbance, calculation sample phytic acid content with spectrophotometer at wavelength 500nm place.
(2) determining the protein quantity method, Kjeldahl's method:
By the water-soluble solution of 50mL for 1g soybean protein isolate SPI, regulate pH to 7.0, magnetic agitation 1h, and then 4000rpm, centrifugal 20min, gets supernatant, carries out kjeldahl determination, surveys protein content.
(3) solubility test method:
Albumen solubility is: (protein content in supernatant crude protein content/bulk sample) * 100%.
Beneficial effect of the present invention: the present invention by gentle, simply, enzymolysis regulates efficiently, makes the soybean protein isolate of preparation not only have lower phytic acid content, also has higher solubility simultaneously under neutral environment.Production process is simple, and cost is lower, and suitability for industrialized production is easy to realize.
Brief description of the drawings
Fig. 1 is that phytic acid content is measured schematic flow sheet.
Detailed description of the invention
In conjunction with specific embodiment below, the present invention is described in further detail, but specific embodiment is not done any restriction to the present invention.
Comparative example 1
Get the defatted soybean meal of 100g, mix with deionized water in the ratio of 1:10 (w/v), stir, regulate pH to 7.0, stablize with this understanding the molten 1h of alkali, carry out afterwards centrifugal 9500rpm, 30min obtains protein solution, regulate pH to 4.5, carry out acid heavy, centrifugal 6000rpm, 30min, obtain curdled milk, separate brokenly after curdled milk and deionized water are dissolved in the ratio of 1:10 (w/v), neutralization makes SPI.Further the phytic acid content to SPI, albumen solubility are investigated to analyze and are obtained: owing to not carrying out enzymolysis, the phytic acid content of gained SPI is up to 19.87mg/g, and albumen solubility is 91.46%.
Comparative example 2
Get the defatted soybean meal of 100g, mix with deionized water in the ratio of 1:10 (w/v), stir, regulate pH to 7.0, stablize with this understanding the molten 1h of alkali, carry out afterwards centrifugal 9500rpm, 30min obtains protein solution, regulate enzymatic hydrolysis condition to add phytase amount 20U/g dregs of beans, pH5.0,55 DEG C of temperature, regulate rapidly pH to 4.5 after time 30min, carry out acid heavy, centrifugal 6000rpm, 30min, obtains curdled milk, after curdled milk and deionized water are dissolved in the ratio of 1:10 (w/v), separate brokenly, neutralization makes SPI.Further the phytic acid content to SPI, albumen solubility are investigated to analyze and are obtained: although phytic acid content is reduced to 0.17mg/g, albumen solubility is but significantly reduced to 68.02%.
Embodiment 1
Get pending dregs of beans, pulverize and sieve; Pulverization conditions is rotating speed 35000rpm, and time 30s pulverizes 3 times, every minor tick 30s, and sieve order number is 100 orders; Get the dregs of beans of 100g, mix with deionized water in the ratio of 1:10 (w/v), stir, regulate pH to 7.0, stablize with this understanding the molten 1h of alkali, carry out afterwards centrifugal 9500rpm, 30min obtains protein solution, regulate enzymatic hydrolysis condition to add phytase amount 25U/g dregs of beans, time 30min, pH5.0,40 DEG C of temperature, regulate rapidly afterwards pH to 4.5, carry out acid heavy, centrifugal 6000rpm, 30min, obtain curdled milk, separate brokenly after curdled milk and deionized water are dissolved in the ratio of 1:10 (w/v), neutralization makes SPI.
Further the phytic acid content to SPI, albumen solubility are investigated to analyze and are obtained: although phytic acid content is reduced to 0.98mg/g, albumen solubility is reduced to 80.65% also.
Embodiment 2
Get pending dregs of beans, pulverize and sieve; Pulverization conditions is rotating speed 30000rpm, and time 1min pulverizes 2 times, every minor tick 30s, and sieve order number is 80 orders; Get the defatted soybean meal of 100g, mix with deionized water in the ratio of 1:10 (w/v), stir, regulate pH to 7.0, stablize with this understanding the molten 1h of alkali, carry out afterwards centrifugal 9500rpm, 30min obtains protein solution, regulate enzymatic hydrolysis condition to add phytase amount 50U/g dregs of beans, time 50min; PH7.0,25 DEG C of temperature, regulate rapidly pH to 4.5 afterwards, carry out acid heavy, centrifugal 6000rpm, 30min, obtains curdled milk, curdled milk and deionized water is separated after by the ratio dissolving of 1:10 (w/v) broken, neutralizes and makes SPI.
Further the phytic acid content to SPI, albumen solubility are investigated to analyze and are obtained: phytic acid content is reduced to 6.57mg/g, but content is still somewhat higher compared with control group, and albumen solubility is 90.38%; Have by a small margin and decline with control group, but change not remarkable.
Embodiment 3
Get pending dregs of beans, pulverize and sieve; Pulverization conditions is rotating speed 30000rpm, and time 1min pulverizes 2 times, every minor tick 30s, and sieve order number is 80 orders; Get the defatted soybean meal of 100g, mix with deionized water in the ratio of 1:10 (w/v), stir, regulate pH to 7.0, stablize with this understanding the molten 1h of alkali, carry out afterwards centrifugal 9500rpm, 30min obtains protein solution, regulate enzymatic hydrolysis condition to add phytase amount 30U/g dregs of beans, time 40min; PH6.5,55 DEG C of temperature, regulate rapidly pH to 4.5 afterwards, carry out acid heavy, centrifugal 6000rpm, 30min, obtains curdled milk, curdled milk and deionized water is separated after by the ratio dissolving of 1:10 (w/v) broken, neutralizes and makes SPI.
Further the phytic acid content to SPI, albumen solubility are investigated to analyze and are obtained: although phytic acid content is reduced to 0.85mg/g, albumen solubility is also reduced to 67.56%.
Embodiment 4
Get pending dregs of beans, pulverize and sieve; Pulverization conditions is rotating speed 25000rpm, and time 2min pulverizes 2 times, every minor tick 30s, and sieve order number is 60 orders; Get the dregs of beans after treatment of 100g, mix with deionized water in the ratio of 1:10 (w/v), stir, regulate pH to 7.0, stablize with this understanding the molten 1h of alkali, carry out afterwards centrifugal 9500rpm, 30min obtains protein solution, regulate enzymatic hydrolysis condition to add phytase amount 20U/g dregs of beans, time 30min, pH5.0,20 DEG C of temperature, regulate rapidly afterwards pH to 4.5, carry out acid heavy, centrifugal 6000rpm, 30min, obtain curdled milk, separate brokenly after curdled milk and deionized water are dissolved in the ratio of 1:10 (w/v), neutralization makes SPI.
Further the phytic acid content to SPI, albumen solubility are investigated to analyze and are obtained: phytic acid content is significantly reduced to 1.27mg/g; Albumen solubility is 92.53%, is improved than control group.Product has the characteristic of low phytic acid, high-dissolvability, has good functional characteristic, can be widely used in the productions such as food, meat products, dairy products processing.
Present invention includes variation and advantage that person skilled in art can expect, taking appending claims as protection domain.
Claims (7)
1. one kind has the preparation method of the low phytic acid soybean protein isolate of high-dissolvability, it is characterized in that: dregs of beans is pulverized and sieved and removed after insoluble fibre impurity, alkaline process extracts and obtains water-soluble soybean protein solution, remove phytic acid through enzymatic hydrolysis, isoelectric precipitation obtains curdled milk, broken through separating, stir, neutralization, the dry soybean protein isolate that makes;
Concrete steps are as follows:
(1) pulverize and sieve: get pending dregs of beans, pulverize and sieve; Pulverization conditions is rotating speed 25000-35000rpm, and time 30s-2min pulverizes 2-3 time, every minor tick 30s, and sieve order number is 60-100 order;
(2) alkaline process extract soybean protein isolate: get step (1) pulverize after dregs of beans water-soluble, dregs of beans is 1:10-20 with water quality ratio, adding aqueous slkali to regulate pH is 7.0-7.5, at temperature 20-50 DEG C, reacts 1-2h, uses after completion of the reaction the gauze of layer 2-4 to filter; To filter gained mixture at the centrifugal 30min of 9500rpm, obtain protein solution stand-by;
(3) enzymolysis: get step (2) gained protein solution, add phytase to carry out enzymolysis, enzyme concentration is 15-200U/g dregs of beans, regulating enzymolysis pH with sour reagent is 4.5-6.5, and hydrolysis temperature is 10-30 DEG C, and enzymolysis time is 30min-2h;
(4) isoelectric precipitation: get product after step (3) enzymolysis and carry out isoelectric point precipitates albumen centrifugal, selected acid heavy with sour reagent adjusting pH be 4.4-4.6, temperature is room temperature, the heavy 30min-1h of acid, centrifugal condition is 5000-9500rpm, and time 15-30min, obtains curdled milk;
(5) post processing: the curdled milk to step (4) gained and deionized water are dissolved in the ratio of 1:10, separates broken and stirs at the high-speed shearing machine of 15000-30000rpm; Then add alkaline agent to neutralize, the finally dry 5-60s of spraying at 60-100 DEG C, obtains product and has the low phytic acid soybean protein isolate of high-dissolvability.
2. the preparation method according to claim 1 with the low phytic acid soybean protein isolate of high-dissolvability, is characterized in that: the described aqueous slkali of step (2) is that concentration is NaOH, potassium hydroxide or the aqua calcis of 2M.
3. the preparation method according to claim 1 with the low phytic acid soybean protein isolate of high-dissolvability, is characterized in that: citric acid, hydrochloric acid or acetic acid that the adjusting pH value sour reagent used that step (3) and (4) are used is 2M for concentration.
4. the preparation method according to claim 1 with the low phytic acid soybean protein isolate of high-dissolvability, is characterized in that: the phytase adding in step (3) is: aspergillus niger or pichia yeast fermenting and producing.
5. the preparation method according to claim 1 with the low phytic acid soybean protein isolate of high-dissolvability, is characterized in that: the described pulverization conditions of step (1) is rotating speed 30000rpm, and the time is 30s, pulverizes twice, every minor tick 30s, and order number is 80 orders.
6. there is according to claim 1 the preparation method of the low phytic acid soybean protein isolate of high-dissolvability, it is characterized in that: the mass ratio of the described dregs of beans of step (2) and water is 1:15, extracting pH is 7.0, at 25 DEG C of temperature, react 1h, the aqueous slkali of selecting is that concentration is NaOH, potassium hydroxide or the aqua calcis of 2M, and the gauze number of plies is two-layer.
7. there is according to claim 1 the preparation method of the low phytic acid soybean protein isolate of high-dissolvability, it is characterized in that: the acid of step (4) heavy with salt acid for adjusting pH be 4.5, temperature is that room temperature, the acid time of sinking is 30min, centrifugal condition is 6000rpm, time 20min, obtains curdled milk.
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| CN107927320A (en) * | 2017-12-20 | 2018-04-20 | 临邑禹王植物蛋白有限公司 | Curdled milk micronization processes apparatus and method in a kind of soybean protein technique |
| CN109349418A (en) * | 2018-12-14 | 2019-02-19 | 中国农业大学 | A kind of preparation method of low irritability low phytic acid deodorization soybean protein isolate |
| CN110250316A (en) * | 2019-04-26 | 2019-09-20 | 维乐维健康产业有限公司 | A kind of fast dissolution Preparation of SPI |
| CN110384231A (en) * | 2019-07-19 | 2019-10-29 | 郑州工程技术学院 | A kind of composite nutrition powder of low phytic acid high dissolubility albumen |
| CN111374216A (en) * | 2018-12-28 | 2020-07-07 | 丰益(上海)生物技术研发中心有限公司 | A method for preparing plant-derived protein isolate and the protein obtained |
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| CN115088784A (en) * | 2022-06-29 | 2022-09-23 | 江西省农业科学院农产品加工研究所 | Natural aggregation method and extraction method of sesame protein |
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| CN107927320A (en) * | 2017-12-20 | 2018-04-20 | 临邑禹王植物蛋白有限公司 | Curdled milk micronization processes apparatus and method in a kind of soybean protein technique |
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| CN110384231A (en) * | 2019-07-19 | 2019-10-29 | 郑州工程技术学院 | A kind of composite nutrition powder of low phytic acid high dissolubility albumen |
| CN113057247A (en) * | 2019-12-31 | 2021-07-02 | 丰益(上海)生物技术研发中心有限公司 | High foaming and foaming stability soy protein composition and method of making same |
| CN113057247B (en) * | 2019-12-31 | 2024-02-02 | 丰益(上海)生物技术研发中心有限公司 | Soybean protein composition with high foamability and foamability stability and preparation method thereof |
| CN115088784A (en) * | 2022-06-29 | 2022-09-23 | 江西省农业科学院农产品加工研究所 | Natural aggregation method and extraction method of sesame protein |
| CN115088784B (en) * | 2022-06-29 | 2023-07-25 | 江西省农业科学院农产品加工研究所 | Natural aggregation method and extraction method of sesame protein |
| CN116391875A (en) * | 2023-04-13 | 2023-07-07 | 东北农业大学 | Soybean protein fibril-beta-carotene gel-based iron supplement and preparation method thereof |
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