CN103954613A - Electrochemical luminescence system with good biocompatibility - Google Patents
Electrochemical luminescence system with good biocompatibility Download PDFInfo
- Publication number
- CN103954613A CN103954613A CN201410196909.9A CN201410196909A CN103954613A CN 103954613 A CN103954613 A CN 103954613A CN 201410196909 A CN201410196909 A CN 201410196909A CN 103954613 A CN103954613 A CN 103954613A
- Authority
- CN
- China
- Prior art keywords
- dipeptides
- nanotube
- concentration range
- solution
- electrochemical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
The invention provides an electrochemical luminescence system with good biocompatibility, and belongs to an electrochemical luminescence system with good biocompatibility. The system comprises: a dipeptide nanotube obtained by self-assembly of a diphenyl alanine dipeptide solution with a concentration range of 0.5-4.0 mg.mL<-1> is used as luminescent substance, and the dipeptide nanotube luminescent substance is modified to the surface of a glassy carbon electrode; co-reaction substance is one of tri-n-propyl amine, ammonium acetate and hexamethylenetetramine, wherein the concentration range of the tri-n-propyl amine is 0.01-20 mmol.L<-1>, the concentration range of the ammonium acetate is 1-20 mmol.L<-1>, and the concentration range of the hexamethylenetetramine is 5-20 mmol.L<-1>; the co-reaction substance exists in a buffer solution, and the pH value of the buffer solution is 6-9. Excited luminescence of the electrochemical luminescent system is analyzed by an electrochemical method. The tubular diameter of the dipeptide nanotube is 400-3000 nm and the length is 80-300 microns. The electrochemical luminescence system has the advantages of good biocompatibility, environmental protection and stable reagent, and can be widely applied to biological analysis.
Description
Technical field
The invention belongs to electrochemiluminescence analysis technical field, particularly a kind of electrochemical luminescence system of good biocompatibility.
Background technology
Electrochemiluminescence becomes a noticeable analytical technology in view of its high sensitivity and the wide range of linearity.In recent years, the application acceleration of nano material the application of electrochemiluminescence technology aspect bioanalysis.Semiconductor nano, as CdSe and CdTe, although in being commonly used in electrochemiluminescence research, the toxicity of its heavy metal element inherence has limited their widespread uses in bioanalysis.For maintaining environment good in bioanalysis, the silicon of low toxicity and carbon nano-crystal and gold nanoclusters are attempted being used in electrochemiluminescence analysis.But, their luminous intensity a little less than, also need further raising.Therefore, development is efficient, the electrochemical luminescence system of good biocompatibility to electrochemiluminescence technology the application in bioanalysis significant.
In recent years, two phenylalanine dipeptides, as the simplest dipeptides primitive, attract wide attention.It is the main identification motif of β-starch albumen of alzheimer disease, can be self-assembled into various structure, makes them to provide abundant construction module for numerous application.Dipeptides nano material has good biocompatibility, is easy to the advantage of synthetic and chemical modification.Dipeptides nanotube (Nano Lett.2009,9,3111-3115) is found in nearest research, dipeptides nanosphere (App.Phys.Lett.2009,94,261907) and gel (Adv.Mater.2010,22,2311-2315) in exist quantum confinement phenomenon.These dipeptides self-assembled nano structures are to form (J.Am.Chem.Soc.2010 by nanocrystalline or quantum dot, 132,15632-15636), make this class Bio-Nano-Materials have outstanding electrical and optical properties, eco-friendly clean optical material can be provided.But the application based on dipeptides nanotube in electrochemiluminescence technology have not been reported.
Summary of the invention
The object of the present invention is to provide a kind of electrochemical luminescence system of good biocompatibility, solved the problem such as destruction mechanics of biological tissue and environmental pollution existing in electrochemiluminescence analysis.
Electrochemical luminescence system of the present invention comprises: be 0.5～4.0mgmL by concentration range
-1the dipeptides nanotube that obtains of two phenylalanine dipeptide solution self assemblies be luminescent substance, dipeptides nanotube luminescent substance is modified to glass-carbon electrode surface; Coreaction material is Tri-n-Propylamine, ammonium acetate, and the one in hexamethylenetetramine, wherein the concentration range of Tri-n-Propylamine is 0.01～20mmolL
-1, the concentration range of ammonium acetate is 1～20mmolL
-1, the concentration range of hexamethylenetetramine is 5～20mmolL
-1; Coreaction material is present in buffer solution, and the pH value of buffer solution is 6～9.Electrochemical luminescence system stimulated luminescence is analyzed with electrochemical method.Wherein, the tubular diameter of dipeptides nanotube is 400～3000nm, and length is 80～300 μ m.
The preparation process of luminescent substance dipeptides nanotube of the present invention is:
At 20～25 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 95～105mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration range that makes dipeptides is 0.5～4.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly.
Coreaction material of the present invention is Tri-n-Propylamine, ammonium acetate, the one in hexamethylenetetramine.Wherein the concentration range of Tri-n-Propylamine is 0.01～20mmolL
-1, the concentration range of ammonium acetate is 1～20mmolL
-1, the concentration range of hexamethylenetetramine is 5～20mmolL
-1.
Buffer solution of the present invention is the phosphate buffered solution of pH6～9.
Electrochemical luminescence system assay method of the present invention adopts three-electrode system, and wherein glass-carbon electrode is working electrode, and platinum filament is to electrode, and silver-silver chloride electrode is contrast electrode.Use electrochemical method to excite before test, need be by nanometer tube modified dipeptides to clean glass-carbon electrode surface, its painting amount at electrode surface is 6 μ L, and scanning potential range is 0～+ 1.4V, and the voltage of photomultiplier is 800V.
Feature of the present invention and effect are:
The invention provides a kind of electrochemical luminescence system of good biocompatibility.The luminescent substance of this electrochemical luminescence system is the dipeptides nanotube that two phenylalanine dipeptide self assemblies form, and coreaction material is Tri-n-Propylamine, ammonium acetate, the one in hexamethylenetetramine.The luminescent system of dipeptides nanotube and amine composition has luminous signal.The advantage of this electrochemical luminescence system good biocompatibility, environmental friendliness, stable reagent, can be widely used in bioanalysis.
Brief description of the drawings
Fig. 1 is the scanning electron microscope (SEM) photograph of the dipeptides nanotube in embodiment 1.
Fig. 2 is the electrochemiluminescence spectrogram of dipeptides nanotube, and emission wavelength is 615nm.
Fig. 3 is the light intensity-voltage curve in embodiment 1, it is blank glass-carbon electrode (a), the nanometer tube modified electrode of dipeptides (b) taking Tri-n-Propylamine in the phosphate buffered solution of coreaction material, carry out that electrochemiluminescence test obtains.
Fig. 4 is the stability of photoluminescence curve map in embodiment 1, be the nanometer tube modified electrode of dipeptides taking Tri-n-Propylamine in the phosphate buffered solution of coreaction material, carry out that electrochemiluminescence test obtains.
Fig. 5 is the light intensity-voltage curve in embodiment 2, is blank glass-carbon electrode (a), the nanometer tube modified electrode of dipeptides (b) taking ammonium acetate in the phosphate buffered solution of coreaction material, carry out that electrochemiluminescence test obtains.
Fig. 6 is the stability of photoluminescence curve map in embodiment 2, be the nanometer tube modified electrode of dipeptides taking ammonium acetate in the phosphate buffered solution of coreaction material, carry out that electrochemiluminescence test obtains.
Fig. 7 is the light intensity-voltage curve in embodiment 3, blank glass-carbon electrode (a), the nanometer tube modified electrode of dipeptides (b) taking hexamethylenetetramine in the phosphate buffered solution of coreaction material, carry out that electrochemiluminescence test obtains.
Fig. 8 is the stability of photoluminescence curve map in embodiment 3, be the nanometer tube modified electrode of dipeptides taking hexamethylenetetramine in the phosphate buffered solution of coreaction material, carry out that electrochemiluminescence test obtains.
Embodiment
At embodiment 1:25 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 100mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration that makes dipeptides is 2.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly, and its scanning electron microscope (SEM) photograph as shown in Figure 1.To glass-carbon electrode surface, containing 10mmolL by nanometer tube modified dipeptides
-1the 0.1molL of Tri-n-Propylamine
-1in the phosphate buffered solution of pH7.4, analyze with electrochemical method stimulated luminescence, light intensity-the voltage curve obtaining as shown in Figure 3, (luminous intensity that Fig. 3 b) records in test fluid is 7500 to the nanometer tube modified glass-carbon electrode of dipeptides, (a) as a comparison, luminous intensity is only 750 to Fig. 3 to blank glass-carbon electrode.As shown in Figure 4, the nanometer tube modified glass-carbon electrode of dipeptides is more stable in test process light intensity for the stability of photoluminescence curve map that this test obtains simultaneously.
At embodiment 2:20 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 105mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration that makes dipeptides is 2.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly, is modified glass-carbon electrode surface, is containing 10mmolL
-1the 0.1molL of ammonium acetate
-1in the phosphate buffered solution of pH7.4, analyze with electrochemical method stimulated luminescence, light intensity-the voltage curve obtaining as shown in Figure 5, (luminous intensity that Fig. 5 b) records in test fluid is 260 to the nanometer tube modified glass-carbon electrode of dipeptides, (Fig. 5 a) as a comparison, does not have luminous signal to blank glass-carbon electrode.As shown in Figure 6, the nanometer tube modified glass-carbon electrode of dipeptides is more stable in test process light intensity for the stability of photoluminescence curve map that this test obtains simultaneously.
At embodiment 3:22 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 95mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration that makes dipeptides is 2.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly, is modified glass-carbon electrode surface, is containing 10mmolL
-1the 0.1molL of hexamethylenetetramine
-1in the phosphate buffered solution of pH7.4, analyze with electrochemical method stimulated luminescence, light intensity-the voltage curve obtaining as shown in Figure 7, (luminous intensity that Fig. 7 b) records in test fluid is 36 to the nanometer tube modified glass-carbon electrode of dipeptides, (Fig. 7 a) as a comparison, does not have luminous signal to blank glass-carbon electrode.As shown in Figure 8, the nanometer tube modified glass-carbon electrode of dipeptides is more stable in test process light intensity for the stability of photoluminescence curve map that this test obtains simultaneously.
At embodiment 4:21 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 105mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration that makes dipeptides is 4.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly, is modified glass-carbon electrode surface, is containing 0.01mmolL
-1three positive third
The 0.1molL of amine
-1in the phosphate buffered solution of pH6.0, analyze with electrochemical method stimulated luminescence, replication three times, the luminous signal mean intensity obtaining is 290.
At embodiment 5:24 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 100mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration that makes dipeptides is 3.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly, is modified glass-carbon electrode surface, is containing 5mmolL
-1the 0.1molL of hexamethylenetetramine
-1in the phosphate buffered solution of pH8.0, analyze with electrochemical method stimulated luminescence, replication three times, the luminous signal mean intensity obtaining is 35.
At embodiment 6:20 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 95mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration that makes dipeptides is 1.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly, is modified glass-carbon electrode surface, is containing 15mmolL
-1the 0.1molL of ammonium acetate
-1in the phosphate buffered solution of pH9.0, analyze with electrochemical method stimulated luminescence, replication three times, the luminous signal mean intensity obtaining is 120.
Claims (2)
1. an electrochemical luminescence system for good biocompatibility, is characterized in that, is 0.5～4.0mgmL by concentration range
-1the dipeptides nanotube that obtains of two phenylalanine dipeptide solution self assemblies be luminescent substance, dipeptides nanotube luminescent substance is modified to glass-carbon electrode surface; Coreaction material is Tri-n-Propylamine, ammonium acetate, and the one in hexamethylenetetramine, wherein the concentration range of Tri-n-Propylamine is 0.01～20mmolL
-1, the concentration range of ammonium acetate is 1～20mmolL
-1, the concentration range of hexamethylenetetramine is 5～20mmolL
-1; Coreaction material is present in buffer solution, and the pH value of buffer solution is 6～9; The tubular diameter of dipeptides nanotube is 400～3000nm, and length is 80～300 μ m.
2. electrochemical luminescence system according to claim 1, is characterized in that, described dipeptides nanotube preparation process is:
At 20～25 DEG C, the freezing powder of two phenylalanine dipeptides is dissolved in in hexafluoroisopropanol, to be mixed with concentration be 95～105mgmL
-1solution, mixes this solution with deionized-distilled water, the concentration range that makes dipeptides is 0.5～4.0mgmL
-1.Dipeptides obtains dipeptides nanotube by self assembly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410196909.9A CN103954613B (en) | 2014-05-11 | 2014-05-11 | A kind of electrochemical luminescence system of good biocompatibility |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410196909.9A CN103954613B (en) | 2014-05-11 | 2014-05-11 | A kind of electrochemical luminescence system of good biocompatibility |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103954613A true CN103954613A (en) | 2014-07-30 |
| CN103954613B CN103954613B (en) | 2016-03-23 |
Family
ID=51331918
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410196909.9A Expired - Fee Related CN103954613B (en) | 2014-05-11 | 2014-05-11 | A kind of electrochemical luminescence system of good biocompatibility |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103954613B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181769A (en) * | 2015-08-27 | 2015-12-23 | 北京化工大学 | Electrochemical cell sensor based on peptide nanotubes/chitosan and preparation method thereof |
| CN107014886A (en) * | 2017-03-08 | 2017-08-04 | 常州大学 | A kind of application of phenylalanine dipeptide self assembly product of zinc ion induction in electrochemistry chiral Recognition field |
| CN109288720A (en) * | 2017-07-24 | 2019-02-01 | 刘崇庆 | Deodorant compositions and preparation method thereof |
| CN109580597A (en) * | 2019-01-28 | 2019-04-05 | 青岛科技大学 | It is a kind of based on the electrochemical luminescence biosensor and its preparation method of DNA nanotube and application |
| CN110911655A (en) * | 2018-09-18 | 2020-03-24 | 中信国安盟固利动力科技有限公司 | Self-assembled super-fast-charging positive electrode material and lithium ion battery thereof |
-
2014
- 2014-05-11 CN CN201410196909.9A patent/CN103954613B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| CELINE VALERY: "Peptide nanotubes: molecular organisations, self-assembly mechanisms and applications", 《SOFT MATTER》, vol. 7, 31 December 2011 (2011-12-31), pages 9583 - 9594 * |
| LIHI ADLER-ABRAMOVICH ET. AL.: "Self-assembled arrays of peptide nanotubes by vapour deposition", 《NATURE NANOTECHNOLOGY》, vol. 4, 18 October 2009 (2009-10-18), pages 849 - 854, XP055021018, DOI: doi:10.1038/nnano.2009.298 * |
| NADAV AMDURSKY ET.AL.: "Elementary Building Blocks of Self-Assembled Peptide Nanotubes", 《JACS》, vol. 132, no. 44, 31 October 2010 (2010-10-31), pages 15632 - 15636, XP055021017, DOI: doi:10.1021/ja104373e * |
| SHANE SCANLON AND AMALIA AGGELI: "Self-assembling peptide nanotubes", 《NANOTODAY》, vol. 3, no. 34, 31 August 2008 (2008-08-31), pages 22 - 30 * |
| 张四方: "《大学化学实验 化学创新实验》", 31 May 2012, article "玻碳电极的使用", pages: 51-60 * |
| 李云辉: "《电化学发光》", 30 November 2007, article "共反应电化学发光", pages: 14-17 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105181769A (en) * | 2015-08-27 | 2015-12-23 | 北京化工大学 | Electrochemical cell sensor based on peptide nanotubes/chitosan and preparation method thereof |
| CN107014886A (en) * | 2017-03-08 | 2017-08-04 | 常州大学 | A kind of application of phenylalanine dipeptide self assembly product of zinc ion induction in electrochemistry chiral Recognition field |
| CN109288720A (en) * | 2017-07-24 | 2019-02-01 | 刘崇庆 | Deodorant compositions and preparation method thereof |
| CN110911655A (en) * | 2018-09-18 | 2020-03-24 | 中信国安盟固利动力科技有限公司 | Self-assembled super-fast-charging positive electrode material and lithium ion battery thereof |
| CN109580597A (en) * | 2019-01-28 | 2019-04-05 | 青岛科技大学 | It is a kind of based on the electrochemical luminescence biosensor and its preparation method of DNA nanotube and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103954613B (en) | 2016-03-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103954613B (en) | A kind of electrochemical luminescence system of good biocompatibility | |
| Niu et al. | Controllable electrochemical/electroanalytical approach to generate nitrogen-doped carbon quantum dots from varied amino acids: pinpointing the utmost quantum yield and the versatile photoluminescent and electrochemiluminescent applications | |
| Xu et al. | Fluorescent nitrogen and sulfur co-doped carbon dots from casein and their applications for sensitive detection of Hg2+ and biothiols and cellular imaging | |
| Canevari et al. | High performance electrochemical sensors for dopamine and epinephrine using nanocrystalline carbon quantum dots obtained under controlled chronoamperometric conditions | |
| Jie et al. | Electrochemiluminescence of CdSe quantum dots for immunosensing of human prealbumin | |
| Wang et al. | A near infrared fluorescence resonance energy transfer based aptamer biosensor for insulin detection in human plasma | |
| Dong et al. | Electrochemiluminescence emission from carbon quantum dot-sulfite coreactant system | |
| Deng et al. | Electrogenerated chemiluminescence of nanomaterials for bioanalysis | |
| Xu et al. | Fabrication of carbon quantum dots and their application for efficient detecting Ru (bpy) 32+ in the solution | |
| Yuan et al. | A fluorescence switch sensor used for D-Penicillamine sensing and logic gate based on the fluorescence recovery of carbon dots | |
| Han et al. | Chemiluminescence and electrochemiluminescence applications of metal nanoclusters | |
| Liu et al. | A novel electrochemiluminescent immunosensor based on CdS-coated ZnO nanorod arrays for HepG2 cell detection | |
| Liang et al. | A novel surface modification strategy of CdTe/CdS QDs and its application for sensitive detection of ct-DNA | |
| CN102749317A (en) | Surface enhanced Raman scattering probe based on carbon nanotube and preparation method thereof | |
| CN103611946B (en) | Preparation method of gold nanocluster fluorescent materials protected by methionine | |
| Wu et al. | A highly sensitive electrochemiluminescence sensor for the detection of l-cysteine based on the rhombus-shaped rubrene microsheets and platinum nanoparticles | |
| Zhu et al. | Functional InP nanocrystals as novel near-infrared fluorescent sensors for mercury ions | |
| CN103901197A (en) | Method and device for detecting carcinoembryonic antigen content | |
| US20140154184A1 (en) | Time-gated fluorescence imaging with si-containing particles | |
| Gao et al. | An “on–off–on” fluorescent nanoprobe for recognition of Cu 2+ and GSH based on nitrogen co-doped carbon quantum dots, and its logic gate operation | |
| Zhang et al. | Aggregation-induced phosphorescence enhancement of Mn-doped ZnS quantum dots: the role of dot-to-dot distance | |
| Liu et al. | Electrochemiluminescent biosensor with DNA link for selective detection of human IgG based on steric hindrance | |
| Mei et al. | Electochemiluminescence of CdTe/CdS quantum dots with triproprylamine as coreactant in aqueous solution at a lower potential and its application for highly sensitive and selective detection of Cu2+ | |
| CN108254343A (en) | Detection probe and preparation method and application thereof | |
| Zhou et al. | Sensitive determination of nucleic acids using organic nanoparticle fluorescence probes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160323 |