CN103952365A - Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof - Google Patents
Phosphorus-accumulating bacterium strain with higher phosphorus removal at low temperature, and screening method and application thereof Download PDFInfo
- Publication number
- CN103952365A CN103952365A CN201410214084.9A CN201410214084A CN103952365A CN 103952365 A CN103952365 A CN 103952365A CN 201410214084 A CN201410214084 A CN 201410214084A CN 103952365 A CN103952365 A CN 103952365A
- Authority
- CN
- China
- Prior art keywords
- phosphorus
- culture medium
- culture
- strain
- polyp bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a phosphorus-accumulating bacterium strain with higher phosphorus removal effect at low temperature, which is characterized in that the collection number of the phosphorus-accumulating bacterium is CCTCC NO:M2014140. The phosphorus-accumulating bacterium strain is screened from Chaohu Lake sediment by low-temperature enrichment culture and a dilution mixed plate method, and can efficiently remove phosphorus at low temperature; the strain can still effectively absorb the carbon source in wastewater at 8 DEG C and convert the carbon source into poly-beta-polyhydroxyalkanoats, and meanwhile, release polyphosphates stored in the cells so as to obtain energy; the poly-beta-polyhydroxyalkanoats in the cells are oxidized in the aerobic process to generate energy, and the strain absorbs abundant phosphorus from the wastewater to as to accumulate the phosphorus in the form of polyphosphates. The strain has wide temperature adaptability, has favorable phosphorus-accumulating effect at 8-30 DEG C, and can be used for wastewater treatment in winter or under cold conditions.
Description
Technical field
The present invention relates to polyP bacteria and screening method and application that a strain still has higher phosphor-removing effect at low temperatures, belong to environmental microorganism field.
Background technology
Water pollution problems is the major issue that countries in the world generally face, and this problem is still serious in China such Yi Ge developing country.In recent decades, China's population sharp increase, urbanization paces are accelerated, and urban wastewater discharge is increasing, and water pollution present situation is very serious, and has the trend of obvious deterioration.Along with increasingly sharpening that water pollutes, the eutrophication problem of water body is more and more outstanding.Since nearly ten years, the ratio of China's eutrophication water rises to 55% from 50%, and oligotrophication's water body ratio drops to 0.53% from 3.2% simultaneously.Body eutrophication can cause the amount reproduction of algae, causes the generation of red tide or wawter bloom phenomenon, reduces the transparency of water body, affects water quality; Algal bloom also can consume the dissolved oxygen in water body, affects other hydrobiological existence, destroys aquatic ecological balance; Part algae can discharge toxic substance in water body simultaneously, harm other biological and HUMAN HEALTH.The major cause of natural water eutrophication is to contain a large amount of nutritive substances, and particularly the waste water of the element such as nitrogen phosphorus is injected in rivers and lakes.In numerous elements, exceeding standard of phosphorus content is considered to one of key factor causing body eutrophication.Therefore, how efficient, economic dephosphorization has become the important research direction in current water prevention and cure of pollution field.
Biological phosphate-eliminating technology is the current phosphorus removing method of widespread use in the world, and its advantage is to avoid a large amount of chemical sludges of producing in chemical phosphorus removal method, reduces sludge bulking phenomenon, and save energy, working cost are lower, and is conducive to the recovery of phosphorus.Biological phosphate-eliminating is mainly that the microorganism that is called as polyP bacteria (Poly-phosphate Accumulating Organisms, PAOs) by a class completes, and this quasi-microorganism all belongs to heterotroph bacterium.Its core be utilize polyP bacteria under anaerobic to discharge phosphorus and under aerobic condition the feature to the excessive absorption of phosphorus, and the phosphorus absorbing under aerobic condition is more than the phosphorus discharging under anaerobic condition, thereby the phosphorus in waste water is removed.Phosphorus content in general bacterial body only has 2% left and right of dry cell weight, and polyP bacteria can excess under aerobic condition by the phosphorus suction body in waste water, make phosphorus content surpass 10%, even can reach 30%.In recent years, people conduct in-depth research microorganism dephosphorization technique, and have designed multiple dephosphorization process.
Yet in the winter time or under the cold condition such as cold district, the effect of biological phosphate-eliminating significantly declines.This is mainly because middle temperature polyP bacteria (the phosphorus accumulation organism playing a major role in biological phosphate-eliminating, PAOs) growth metabolism under cold condition is in holddown, and low temperature PAOs under normal temps than in warm PAOs few, metabolic rate is slow, is difficult to form dominant microflora under state of nature.Therefore, realize under cold condition effectively dephosphorization, can not be only from aspect researchs such as research and development technique and operation adjustment, also must be from the feasibility of microbiological angle research and inquirement low-temperature biological dephosphorization.
In recent years, people have carried out certain research to low temperature polyP bacteria, are mainly to tame by thermograde, and therefrom in warm polyP bacteria, screening still has the bacterial strain of poly-phosphorus usefulness at low temperature.Yet the research of this bacterioid and dephosphorization characteristics thereof is all in the junior stage, do not cultivate efficiently acclimation, do not have systematic authentication method yet.Verify kind and the characteristic of low temperature polyP bacteria, define their dephosphorization condition, will contribute to research, the development and application of waste water dephosphorization technique.
Summary of the invention
For above problem, the object of this invention is to provide a strain and still there is at low temperatures the polyP bacteria of higher phosphor-removing effect.This bacterium has thermal adaptability widely, can effectively utilize various carbon sources simultaneously.Another object of the present invention is to provide screening method and the application of this bacterium.
Bacterial strain of the present invention is to utilize low temperature enrichment culture and dilution to mix flat band method from the bed mud of Chaohu, to screen a kind of polyP bacteria that at low temperatures can efficient dephosphorization obtaining, the carbon source that still can effectively absorb when anaerobism at 8 ℃ in waste water is converted into poly-beta-hydroxy alkyl salt, discharge the polyphosphoric acid salt storing in born of the same parents simultaneously, therefrom obtain energy; When aerobic, be oxidized the poly-beta-hydroxy alkyl salt generate energy in born of the same parents, and from waste water, take the photograph in large quantities phosphorus, with the form of polyphosphoric acid salt, accumulated.
The present invention adopts following technical scheme:
One of the object of the invention is to provide a strain to have at low temperatures the polyP bacteria of higher phosphor-removing effect, and the preserving number of described polyP bacteria is CCTCC NO:M2014140.
Another object of the present invention is to provide a kind of screening to have at low temperatures the method for higher phosphor-removing effect polyP bacteria, and its feature is, described method comprises the steps:
(1) prepare respectively culture medium A, B and C:
Culture medium A is strains separation, purifying, storage medium, and it consists of: every liter of culture medium A comprises peptone 10g, extractum carnis 5g, and sodium-chlor 5g, agar 19g, all the other are water, regulate pH to 7.0-7.2,
Substratum B is pre-culture medium, and it consists of: every liter of substratum B comprises sodium acetate 2g, Na
2hPO
446mg, NH
4cl152.8mg, CaCl
28.3mg, MgSO
482mg, K
2sO
417.83mg, HEPES7g, micro-2mL, all the other are water, regulate pH to 7.0-7.2;
Culture medium C is rich phosphorus substratum, and it consists of: every liter of culture medium C comprises sodium acetate 1g, MgSO
482mg, FeSO
43.7mg, CaCl
260mg, (NH
4)
2sO
40.2g, extractum carnis 0.22g, K
2hPO
474mg, micro-2mL, all the other are water, regulate pH to 7.0-7.2;
Consisting of of described trace element: every liter of trace element comprises FeCl
30.9g, H
3bO
3150mg, CuSO
430mg, KI180mg, MnCl
260mg, ZnSO
4120mg, CoCl
2140mg, Na
2moO
460mg, ethylenediamine tetraacetic acid (EDTA) 10g, all the other are water;
(2) enrichment culture
Getting active sludge 0.5g is added in 100mL sterilized water in 6 ℃ of-10 ℃ of shaking culture 24h, the mixed solution of getting after 10mL shaking culture is transferred in the culture medium A that 100mL phosphorus concentration is 5mg/L, 5-9 ℃ of enrichment culture, every cultivation 2d switching once, phosphorus concentration in culture medium A of every switching improves 5mg/L, corotation connects 4 times, and enrichment culture 8d obtains enrichment culture liquid;
(3) separation and purification
Described enrichment culture liquid to extent of dilution is reached to 10
-6, draw 0.5ml diluent in separation and purification flat board, at 40 ℃-42 ℃, mix with culture medium A, solidify rear inversion, in 8 ℃ of constant temperature culture, go out bacterium colony; Single bacterium colony described in picking on flat board purifying of repeatedly ruling on flat board, shows without miscellaneous bacteria to microscopic examination, obtains purifying bacterial strain;
(3) screening
By described purifying inoculation in the Erlenmeyer flask of 100mL substratum B is housed, at 130r/min, shaking culture 24h at 8 ℃, obtain preculture liquid, described preculture liquid is transferred in the Erlenmeyer flask that 100mL culture medium C is housed by 10%, at 130r/min, shaking culture 2d at 8 ℃; Wherein first anaerobism is cultivated 24h aerobic cultivation 24h again; After cultivation completes, get the centrifugal 10min of nutrient solution 10000r/min and get supernatant liquor, measure the total phosphorus concentration of supernatant liquor, investigate the clearance of bacterial strain to total phosphorus, thus the polyP bacteria at low temperatures with higher phosphor-removing effect filtering out.
Screening method of the present invention can filter out the bacterial strain of preservation of the present invention, but is only not limited to, for screening bacterial strain of the present invention, also can be used for the screening that other has higher phosphor-removing effect polyP bacteria at low temperatures.
Another object of the present invention is to the application of polyP bacteria in wastewater treatment.
PolyP bacteria 16SrDNA sequence of the present invention is as shown in SEQ ID NO:1.
Bacterial strain of the present invention has the following advantages:
(1) bacterial strain of the present invention has thermal adaptability widely, has preferably poly-phosphorus effect in 8-30 ℃, can be used for the wastewater treatment under winter or cold condition.
(2) bacterial strain of the present invention has very high dephosphorization activity, and this bacterial strain is after cultivating 48h, and dephosphorizing rate reaches 94.5, can be used for natural water body ecological reconstruction or original position reparation.
Bacterial strain of the present invention is by contriver, from the bed mud of Chaohu, to be screened acquisition in October, 2012, through identifying, belong to Rhodopseudomonas, it is a fluorescent pseudomonads, called after pseudomonas D6Pseudomonas SP.D6, in the center preservation of Chinese Typical Representative culture collection, preservation address: Wuhan, China Wuhan University; Preservation date is on April 20th, 2014; Deposit number is CCTCC NO:M2014140.
Accompanying drawing explanation
Fig. 1 is that bacterial strain of the present invention is cultivated after 48h under differing temps, OD value and the dephosphorizing rate of substratum.
Fig. 2 is that bacterial strain of the present invention is cultivated after 48h in different carbon source substratum, OD value and the dephosphorizing rate of substratum.
Fig. 3 is that bacterial strain of the present invention is cultivated after 48h in different pH value substratum, OD value and the dephosphorizing rate of substratum.
Fig. 4 is OD value and the real-time detected result figure of dephosphorizing rate of bacterial strain of the present invention substratum in culturing process.
Embodiment
Below by the drawings and specific embodiments, the invention will be further described.
Separation screening and the evaluation of embodiment 1 bacterial strain of the present invention:
(1) substratum
Culture medium A: every liter of substratum is containing peptone 10g, extractum carnis 5g, sodium-chlor 5g, agar 19g; Surplus is water; PH7.0-7.2.
Culture medium A is for strains separation, purifying, preservation.
Substratum B: every liter of substratum is containing sodium acetate (carbon source) 2g, Na
2hPO
446mg, NH
4cl152.8mg, CaCl
28.3mg, MgSO
482mg, K
2sO
417.83mg, HEPES7g, micro-2mL; Surplus is water, pH7.0-7.2.
Substratum B is for the pre-culture medium of bacterial strain.
Culture medium C: every liter of substratum is containing sodium acetate (carbon source) 1g, MgSO
482mg, FeSO
43.7mg, CaCl
260mg, (NH
4)
2sO
40.2g, extractum carnis 0.22g, K
2hPO
474mg, micro-2mL; Surplus is water; PH7.0-7.2.
Culture medium C is cultivated for the rich phosphorus of bacterial strain.
D, trace element form (g/L): every liter of substratum is containing FeCl
30.9g, H
3bO
3150mg, CuSO
430mg, KI180mg, MnCl
260mg, ZnSO
4120mg, CoCl
2140mg, Na
2moO
460mg, ethylenediamine tetraacetic acid (EDTA) 10g.
Before above-mentioned substratum is used, 121 ℃, sterilizing 20 minutes.
(2) separation of Pseudomonas fluorescens D6 bacterial strain, purifying and screening:
Under cold condition, carry out enrichment culture.Get Chaohu bed mud 0.5g and be added in 100mL sterilized water, shaking culture 24h at 8 ℃, gets 10mL mixed solution and is transferred to 100mL and adds phosphorus to phosphorus concentration to reach low temperature enrichment culture in 5mg/L beef-protein medium.Once, the phosphorus concentration in beef-protein medium of every switching improves 5mg/L in every cultivation 2d switching, and corotation connects 4 times, enrichment culture 8d, and enrichment obtains the poly-stronger bacterial strain of phosphorus ability.
Adopt mixed diluting flat band method and plate streak to carry out separation and purification.With liquid-transfering gun, draw 0.5ml enrichment culture liquid in the test tube of 4.5ml sterilized water is housed, mix, the rest may be inferred.Finally make the extent of dilution of enrichment culture liquid reach 10
-6.Draw the diluent after dilution, with the amount of every dull and stereotyped 0.5ml, mix with 40 ℃ of left and right isolation mediums, be positioned over Bechtop it is solidified.Afterwards flat board is inverted, is put into 8 ℃ of constant incubators and cultivate.
Picking list bacterium colony, and purifying that the single strain of picking is repeatedly rule on flat board, without till miscellaneous bacteria, now can think that bacterial strain purifying is complete to microscopic examination demonstration.
By the purifying inoculation of gained in the Erlenmeyer flask of 100mL pre-culture medium is housed, at 130r/min, shaking culture 24h at 8 ℃.Preculture liquid is transferred in the Erlenmeyer flask that the rich phosphorus substratum of 100mL is housed by 10%, at 130r/min, shaking culture 2d at 8 ℃.In culturing process, front 24h anaerobism is cultivated, and anaerobic condition seals by rubber plug, and in extracting bottle with sterilized syringe, the air high pure nitrogen that reinjects reaches.The aerobic cultivation of rear 24h of cultivating, is, in Bechtop, rubber plug is changed to air-permeable envelope, and oxygen is entered.After cultivation completes, get the centrifugal 10min of nutrient solution 10000r/min and get supernatant liquor, measure total phosphorus concentration, investigate the clearance of bacterial strain to total phosphorus, the efficient polyP bacteria filtering out is the Pseudomonas fluorescens D6 in the present invention, is seeded to slant medium and preserves.
(3) colony morphology characteristic of Pseudomonas fluorescens D6:
In culture medium A, cultivate bacterium colony oyster white after 2 days, be rule circle, intermediate projections, edge is opaque, glossy.
(4) Molecular Identification of 16SrDNA
Experimental technique: picking thalline from the inclined-plane of bacterial strain D6, add to containing in the centrifuge tube of 100 μ L distilled waters, vortex mixes, and thermo-cracking bacteria suspension be take genomic dna as template amplification 16SrDNA.
Forward primer is 7F:5 '-CAGAGTTTGATCCTGGCT-3 ';
Reverse primer is 1540R:5 '-AGGAGGTGATCCAGCCGCA-3 '.
Amplification reaction system is: DNA profiling 0.5 μ L, 5 * Buffer damping fluid, 2.5 μ L, dNTP1 μ L, positive each 0.5 μ L of anti-primer, enzyme 0.2 μ L, distilled water 19.8 μ L.
Reaction conditions is: 98 ℃ of denaturation 3min, 98 ℃ of 25sec, 55 ℃ of 25sec, 72 ℃ of 1min, 30 circulations, 4 ℃ of preservations after 72 ℃ of prolongation 10min.
With 1% sepharose, the 16SrDNA amplified production of bacterial strain is done to electrophoresis detection, after checking, cut adhesive tape, adopt DNA gel to reclaim test kit purified pcr product.Pcr amplification product entrusts Shanghai Sheng Gong biotechnology company limited to check order.
(5) 16SrDNA sequential analysis and Phylogenetic Analysis:
The sequence that the 16SrDNA length that obtains D6 bacterial strain after order-checking is 1451bp, be submitted to NCBI Genbank database and carry out Blast comparison, the evolutionary distance of finding bacterial strain D6 and Pseudomonas fluorescens is the most approaching, determine that it is Rhodopseudomonas, it is Pseudomonas fluorescens for preliminary identification, called after Pseudomonas fluorescens D6.
D6 bacterial strain 16SrDNA sequence is as shown in SEQ ID NO:1.
The best dephosphorization condition of embodiment 2 bacterial strains of the present invention
1, the seed liquor obtaining by the strain culturing of preservation of the present invention by volume 1:10 is inoculated in culture medium C, shaking culture 48h at 8 ℃, 10 ℃, 15 ℃, 20 ℃, 25 ℃, 30 ℃ respectively, wherein first anaerobism is cultivated 24h, aerobic cultivation 24h again, the OD value and the dephosphorizing rate that after 48h, detect culture medium C, detected result as shown in Figure 1.From Fig. 1 result, bacterial strain of the present invention has thermal adaptability widely, has preferably poly-phosphorus effect in 8-30 ℃, can be used for the wastewater treatment under winter or cold condition.
2, the seed liquor obtaining by the strain culturing of preservation of the present invention by volume 1:10 is inoculated in the rich phosphorus substratum of different carbon sources, at 20 ℃ of shaking culture 48h, wherein first anaerobism is cultivated 24h, more aerobic cultivation 24h, the OD value and the dephosphorizing rate that after 48h, detect culture medium C, detected result as shown in Figure 2.The rich phosphorus substratum of above-mentioned different carbon sources refers to culture medium C, with etc. the resulting culture medium C 1 of sodium acetate in carbon content glucose substitutive medium C, with etc. the resulting culture medium C 2 of sodium acetate in carbon content fructose substitutive medium C, with etc. the resulting culture medium C 3 of sodium acetate in carbon content cane sugar substitution culture medium C, with etc. the resulting culture medium C 4 of sodium acetate in carbon content lactose substitutive medium C; With etc. the resulting culture medium C 5 of sodium acetate in carbon content starch in replace culture medium C.From Fig. 2 result, bacterial strain of the present invention can well utilize various carbon sources, and the suitableeest carbon source is sodium acetate.
3, the seed liquor obtaining by the strain culturing of preservation of the present invention by volume 1:10 is inoculated in the substratum of different pH values, shaking culture 48h at 20 ℃, wherein first anaerobism is cultivated 24h, more aerobic cultivation 24h, the OD value and the dephosphorizing rate that after 48h, detect culture medium C, detected result as shown in Figure 3.The substratum of the different pH values of above-mentioned difference refers to respectively: culture medium C; Culture medium C 6: composition is identical with culture medium C, pH5.0; Culture medium C 7: composition is identical with culture medium C, pH6.0; Culture medium C 8: composition is identical with culture medium C, pH8.0; Culture medium C 9: composition is identical with culture medium C, pH9.0; Culture medium C 10: composition is identical with culture medium C, pH10.0; Culture medium C 11: composition is identical with culture medium C, pH11.0; From Fig. 3 result, bacterial strain of the present invention, in the scope of pH7-9, has very strong dephosphorization active.
4, the seed liquor obtaining by the strain culturing of preservation of the present invention by volume 1:10 is inoculated in culture medium C, shaking culture 48h at 20 ℃, wherein first anaerobism is cultivated 36h, aerobic cultivation 12h again, as shown in Figure 4, after 48h, dephosphorizing rate reaches 94.5% for real-time OD value in whole culturing process and dephosphorizing rate.
Claims (4)
1. a strain has the polyP bacteria of higher phosphor-removing effect at low temperatures, it is characterized in that, the preserving number of described polyP bacteria is CCTCC NO:M2014140.
2. screening has a method for higher phosphor-removing effect polyP bacteria at low temperatures, it is characterized in that, described method comprises the steps:
(1) prepare respectively culture medium A, B and C:
Culture medium A is strains separation, purifying, storage medium, and it consists of: every liter of culture medium A comprises peptone 10g, extractum carnis 5g, and sodium-chlor 5g, agar 19g, all the other are water, regulate pH to 7.0-7.2,
Substratum B is pre-culture medium, and it consists of: every liter of substratum B comprises sodium acetate 2g, Na
2hPO
446mg, NH
4cl152.8mg, CaCl
28.3mg, MgSO
482mg, K
2sO
417.83mg, HEPES7g, micro-2mL, all the other are water, regulate pH to 7.0-7.2;
Culture medium C is rich phosphorus substratum, and it consists of: every liter of culture medium C comprises sodium acetate 1g, MgSO
482mg, FeSO
43.7mg, CaCl
260mg, (NH
4)
2sO
40.2g, extractum carnis 0.22g, K
2hPO
474mg, micro-2mL, all the other are water, regulate pH to 7.0-7.2;
Consisting of of described trace element: every liter of trace element comprises FeCl
30.9g, H
3bO
3150mg, CuSO
430mg, KI180mg, MnCl
260mg, ZnSO
4120mg, CoCl
2140mg, Na
2moO
460mg, ethylenediamine tetraacetic acid (EDTA) 10g, all the other are water;
(2) enrichment culture
Getting active sludge 0.5g is added in 100mL sterilized water in 6 ℃ of-10 ℃ of shaking culture 24h, the mixed solution of getting after 10mL shaking culture is transferred in the culture medium A that 100mL phosphorus concentration is 5mg/L, 5-9 ℃ of enrichment culture, every cultivation 2d switching once, phosphorus concentration in culture medium A of every switching improves 5mg/L, corotation connects 4 times, and enrichment culture 8d obtains enrichment culture liquid;
(3) separation and purification
Described enrichment culture liquid to extent of dilution is reached to 10
-6, draw 0.5ml diluent in separation and purification flat board, at 40 ℃-42 ℃, mix with culture medium A, solidify rear inversion, in 8 ℃ of constant temperature culture, go out bacterium colony; Single bacterium colony described in picking on flat board purifying of repeatedly ruling on flat board, shows without miscellaneous bacteria to microscopic examination, obtains purifying bacterial strain;
(3) screening
By described purifying inoculation in the Erlenmeyer flask of 100mL substratum B is housed, at 130r/min, shaking culture 24h at 8 ℃, obtain preculture liquid, described preculture liquid is transferred in the Erlenmeyer flask that 100mL culture medium C is housed by 10%, at 130r/min, shaking culture 2d at 8 ℃; Wherein first anaerobism is cultivated 24h aerobic cultivation 24h again; After cultivation completes, get the centrifugal 10min of nutrient solution 10000r/min and get supernatant liquor, measure the total phosphorus concentration of supernatant liquor, investigate the clearance of bacterial strain to total phosphorus, thus the polyP bacteria at low temperatures with higher phosphor-removing effect filtering out.
3. the application of polyP bacteria in wastewater treatment described in claim 1.
4. a strain according to claim 1 has the polyP bacteria of higher phosphor-removing effect at low temperatures, it is characterized in that, described polyP bacteria 16SrDNA sequence is as shown in SEQ ID NO:1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410214084.9A CN103952365B (en) | 2014-05-19 | 2014-05-19 | One strain has the polyP bacteria of higher phosphor-removing effect and screening method thereof and application at low temperatures |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410214084.9A CN103952365B (en) | 2014-05-19 | 2014-05-19 | One strain has the polyP bacteria of higher phosphor-removing effect and screening method thereof and application at low temperatures |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103952365A true CN103952365A (en) | 2014-07-30 |
| CN103952365B CN103952365B (en) | 2016-02-10 |
Family
ID=51329722
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410214084.9A Active CN103952365B (en) | 2014-05-19 | 2014-05-19 | One strain has the polyP bacteria of higher phosphor-removing effect and screening method thereof and application at low temperatures |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103952365B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107129982A (en) * | 2017-04-06 | 2017-09-05 | 南华大学 | A kind of preparation method and application of polyP bacteria trace charcoal |
| CN111955387A (en) * | 2020-08-10 | 2020-11-20 | 安徽大学 | Polder area paddy field coupling pond and ditch three-level wetland system |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101386822A (en) * | 2007-09-14 | 2009-03-18 | 哈尔滨工业大学深圳研究生院 | Special effect phosphate accumulating organisms and waste water processing method using thereof |
| CN102776145A (en) * | 2012-07-31 | 2012-11-14 | 山东大学 | Denitrifying polyphosphate accumulation bacterium and application of same in sewage treatment |
-
2014
- 2014-05-19 CN CN201410214084.9A patent/CN103952365B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101386822A (en) * | 2007-09-14 | 2009-03-18 | 哈尔滨工业大学深圳研究生院 | Special effect phosphate accumulating organisms and waste water processing method using thereof |
| CN102776145A (en) * | 2012-07-31 | 2012-11-14 | 山东大学 | Denitrifying polyphosphate accumulation bacterium and application of same in sewage treatment |
Non-Patent Citations (1)
| Title |
|---|
| 王春丽等: "一株耐低温反硝化聚磷菌的筛选及其特性研究", 《环境工程学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107129982A (en) * | 2017-04-06 | 2017-09-05 | 南华大学 | A kind of preparation method and application of polyP bacteria trace charcoal |
| CN107129982B (en) * | 2017-04-06 | 2021-02-02 | 南华大学 | Preparation method and application of phosphorus-accumulating bacterium imprinted biochar |
| CN111955387A (en) * | 2020-08-10 | 2020-11-20 | 安徽大学 | Polder area paddy field coupling pond and ditch three-level wetland system |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103952365B (en) | 2016-02-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105861359A (en) | Heterotrophic nitrification-aerobic denitrification high temperature resisting strain for producing floc, and application thereof | |
| CN104911130A (en) | Halomonas sp. with denitrogenation capability and application thereof | |
| CN114703095B (en) | Pseudomonas adulthood and application thereof in field of sewage and wastewater purification | |
| CN104845920A (en) | Marine zobellella sp. and application thereof | |
| CN102154143A (en) | Marine aerobic denitrifying halomonas strain HGMN422 and application thereof | |
| CN108587970A (en) | A kind of acid-producing Klebsiella bacterium and its application in promoting RADIX CODONOPSIS PILOSULAE from Shanxi of China's growth | |
| CN103114062A (en) | Denitrifying phosphate-accumulating organism with nitrogen and phosphorus removal functions and applications thereof | |
| CN104862260A (en) | Arthrobacter with aerobic denitrification capability and application thereof | |
| CN103103153A (en) | Anaerobic denitrification phosphorus-accumulating bacteria strain with denitrification and phosphorous removal effects and application thereof | |
| CN101591628A (en) | Acinetobacter junii X8 and the application in the preparation algin catenase thereof | |
| CN104726366A (en) | Denitrifying phosphorus accumulation organism (DPAO) with function of efficiently removing nitrogen and phosphorus and application of DPAO | |
| CN108611291A (en) | One plant of salt tolerance planococcus and its application | |
| CN101811779B (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN109337832A (en) | A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application | |
| CN110846254A (en) | Compound microbial agent for denitrification and preparation method and application thereof | |
| CN102676431B (en) | Denitrifying bacteria and aquatic plant-microbe combined rehabilitation method using same | |
| CN109706096A (en) | One plant of brevibacterium frigoritolerans and its application with denitrogenation and efficient flocculating ability | |
| CN103952365B (en) | One strain has the polyP bacteria of higher phosphor-removing effect and screening method thereof and application at low temperatures | |
| CN102864098A (en) | Denitrification phosphorus removal bacterium H-hrb02 as well as screening method and application thereof | |
| CN102994394B (en) | Fungal strain LP-18-3 and application of fungal strain LP-18-3 in lead-containing water body treatment | |
| CN104694435B (en) | One plant of Shinella sp. with triazole degradation function and its application | |
| CN115353210B (en) | Application of bacillus pumilus LZP02 in treatment of pig raising wastewater | |
| CN103011423A (en) | Application of Bacillus cereus DS1 in degradation of organic pollutants in saponin waste water | |
| CN106591181B (en) | A kind of Mysore arthrobacterium and its application in purifying sea water cultivation nitrogenous effluent | |
| CN105754904B (en) | One plant of ocean Psychrobacter strain and its application in water body dephosphorized |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |