CN103951761B - The method of enzymic degradation sea grass polysaccharide - Google Patents
The method of enzymic degradation sea grass polysaccharide Download PDFInfo
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Abstract
The invention discloses a kind of method of enzymic degradation sea grass polysaccharide: Enteromorpha Crude polysaccharides or sea grass polysaccharide are configured to the polysaccharide soln that concentration is 1 ~ 20mg/mL; Then using saccharifying enzyme as lytic enzyme, in pH be 3.5 ~ 5.5, temperature is 40 ~ 60 DEG C of reactions 1 ~ 5 hour, enzymolysis degradation of polysaccharide solution first; After enzymolysis degradation of polysaccharide solution boils 4 ~ 6 minutes first, centrifugal to remove saccharifying enzyme, the supernatant liquor of the rear sea grass polysaccharide of enzymolysis degraded first must be contained.By above-mentioned supernatant liquor through dialysis postlyophilization, obtain pulverous enzymolysis degraded sea grass polysaccharide (molecular weight ranges is 7.2 ~ 122kDa).It is high that method of the present invention has hydrolysis efficiency, the feature that gained polysaccharide antioxidation activity in vitro is good.
Description
Technical field
The invention belongs to technical field of food biotechnology, relate to a kind of method of enzymic degradation sea grass polysaccharide.
Background technology
Enteromorpha is the large-scale economy green alga of one of China's southeastern coast, aboundresources, and itself is edible, and containing various active material, as sea grass polysaccharide, lipid pigment, phenols etc.According to the literature, sea grass polysaccharide has and improves mammalian immune power, reducing blood-fat, antiphlogistic antibacterial and the physiological function such as anti-oxidant.Therefore Enteromorpha is developed to functional food and has good prospect.Enteromorpha water extraction polysaccharide is mainly water miscible sulfated polysaccharide, and it forms mainly glucuronic acid-xylose-rhamnose polymkeric substance.Sea grass polysaccharide molecular weight is large, if be degraded into the relatively little product of molecular weight, then owing to dissociating more active group, can its biological activity can be made to be improved.
It is reported, sea grass polysaccharide sugar key type mainly β-Isosorbide-5-Nitrae, α-1,2, α-Isosorbide-5-Nitrae, α-1,2,4 and β-1,6, the β-1,3 also containing small amount and α-1,3 glycosidic link.
Acid degradation is the traditional method of polysaccharide degraded, by adjustment acidity, temperature and the hydrolysate obtaining different palliating degradation degree action time.But sulfated polysaccharide its sulfate in degradation process is easily hydrolyzed and comes off, the reduction of sulfate content can cause the physiologically active of polysaccharide to reduce; The degradation rate of the method is 40.05%; Molecular weight ranges is generally 5.32 ~ 95.6kDa.
Come off though oxidative degradation can reduce the sulfate of polysaccharide in degradation process, oxidation products is relatively complicated, adds the difficulty of separation and purification.The degradation rate of the method is 61.32%; Molecular weight ranges is generally 2.28 ~ 55.9kDa.
Enzymic degradation action condition is gentle, also can avoid coming off of sulfate, but be difficult to obtain the high enzyme of hydrolysis efficiency.But at present the enzymic degradation of sea grass polysaccharide is seldom had been reported.
Summary of the invention
The technical problem to be solved in the present invention is to provide the method for the enzymic degradation sea grass polysaccharide that a kind of hydrolysis efficiency is high, antioxidation activity in vitro is good.
In order to solve the problems of the technologies described above, the invention provides a kind of method of enzymic degradation sea grass polysaccharide, Enteromorpha Crude polysaccharides or sea grass polysaccharide are configured to the polysaccharide soln that concentration is 1 ~ 20mg/mL (being preferably 4 ~ 6mg/mL); Then using saccharifying enzyme (glucoamylase) as lytic enzyme, in polysaccharide soln, saccharifying enzyme is added until the final concentration of saccharifying enzyme is 2 ~ 100U/mL (being preferably 10 ~ 20U/mL); In pH be 3.5 ~ 5.5, temperature is 40 ~ 60 DEG C of reactions 1 ~ 5 hour (be preferably in pH be 4.5,49 DEG C of reactions 2 hours), enzymolysis degradation of polysaccharide solution first; After enzymolysis degradation of polysaccharide solution boils 4 ~ 6 minutes first, centrifugal to remove saccharifying enzyme, the supernatant liquor of the rear sea grass polysaccharide of enzymolysis degraded first must be contained.
Remarks illustrate: Enteromorpha Crude polysaccharides or sea grass polysaccharide are configured to the polysaccharide soln that concentration is 1 ~ 20mg/mL, are: when selecting Enteromorpha Crude polysaccharides, the concentration of Enteromorpha Crude polysaccharides is 1 ~ 20mg/mL; When selecting sea grass polysaccharide, the concentration of sea grass polysaccharide is 1 ~ 20mg/mL.
Centrifugally to refer to: the centrifugal 4 ~ 6min of 7000 ~ 9000r/s.
Improvement as the method for enzymic degradation sea grass polysaccharide of the present invention: by being the dialysis tubing dialysis of 500 containing the supernatant liquor molecular weight cut-off of sea grass polysaccharide after enzymolysis degraded first, to remove inorganic salt; Obtain dialyzate; By dialyzate lyophilize, obtain pulverous enzymolysis degraded sea grass polysaccharide (molecular weight is lower, and molecular weight ranges is between 7.2 ~ 122kDa).
Remarks illustrate: above-mentioned dialysis time is 20 ~ 28 hours; Lyophilize refers to-45 ~-55 DEG C of dryings 20 ~ 28 hours.
Another kind as the method for enzymic degradation sea grass polysaccharide of the present invention improves: be again hydrolyzed containing the supernatant liquor of sea grass polysaccharide after enzymolysis degraded first, described hydrolysis again comprises the steps:
In the supernatant liquor containing the rear sea grass polysaccharide of enzymolysis degraded first, add polygalacturonase, the consumption of described polygalacturonase is 1 ~ 10% (being preferably 6 ~ 9%) of Enteromorpha Crude polysaccharides or sea grass polysaccharide weight; In pH be 3.5 ~ 5.5, temperature is 45 ~ 60 DEG C of reactions 1 ~ 5 hour (being preferably pH is 4.5, and temperature is 56 DEG C of reactions 3 hours), enzymolysis degradation of polysaccharide solution again; After enzymolysis degradation of polysaccharide solution boils 4 ~ 6 minutes again, centrifugal to remove polygalacturonase, the supernatant liquor of the rear sea grass polysaccharide of enzymolysis degraded must be contained again.
Remarks illustrate: the enzyme of polygalacturonase is lived as 30U/mg.Centrifugally to refer to: the centrifugal 4 ~ 6min of 7000 ~ 9000r/s.
Further improvements in methods as enzymic degradation sea grass polysaccharide of the present invention:
By the dialysis tubing dialysis that the supernatant liquor molecular weight cut-off containing again the rear sea grass polysaccharide of enzymolysis degraded is 500, to remove inorganic salt; Obtain dialyzate; By dialyzate lyophilize, obtain pulverous enzymolysis degraded sea grass polysaccharide (molecular weight is lower, and molecular weight ranges is between 7.2 ~ 122kDa).
Remarks illustrate: above-mentioned dialysis time is 20 ~ 28 hours; Lyophilize refers to-45 ~-55 DEG C of dryings 20 ~ 28 hours.
In the present invention, the acetic acid-sodium acetate buffer solution of available pH3.5 ~ 5.5 carries out the regulable control of pH value.
The purity (mass content) of Enteromorpha Crude polysaccharides used in the present invention is 75 ~ 80%.
In the present invention, Enteromorpha Crude polysaccharides or sea grass polysaccharide all obtain by known technology, and such as, Enteromorpha Crude polysaccharides also obtains by following method preparation:
1), by the Enteromorpha stamp mill after drying (water ratio≤8.21%, quality %) pulverize, cross 120 mesh sieves, obtain Enteromorpha powder.
2), ethanol removal of impurities:
By dehydrated alcohol and the Enteromorpha powder solid-liquid ratio according to 20ml/1g, stir under 90 DEG C of water bath condition thus fat-soluble component in removing marine alga, described dedoping step repeats 2 ~ 4 times (each stir about 30 minutes), filters, gets filter cake.
3), water extraction sea grass polysaccharide:
By step 2) filter cake of gained according to " Enteromorpha water-soluble polysaccharide extraction process " in " technical study that Enteromorpha water-soluble polysaccharide extracts ", prepare the Enteromorpha Crude polysaccharides aqueous solution.
4), the removal of albumen:
By step 3) the Enteromorpha Crude polysaccharides aqueous solution of gained carries out enzyme process deproteinated according to " the sea grass polysaccharide enzyme process deproteinated technique " in " research of sea grass polysaccharide method for removing protein ": the consumption of papoid is 3% (W:V), pH value is 6.5, temperature 50 C, enzymolysis time is 2.5h.
Again the enzymolysis solution of gained is utilized Sevag method removing protein repeatedly, it is centrifugal until upper strata is almost without albumen, vacuum rotary steam removing chloroform and propyl carbinol, adding dehydrated alcohol makes its purity be not less than 80%, after 4 DEG C of refrigerator alcohol precipitation 12h, more centrifugal, the order of precipitation dehydrated alcohol, ether, acetone is repeatedly washed, finally carry out lyophilize, obtain Enteromorpha Crude polysaccharides.Wherein, the mass content of polysaccharide is 75.45%, and namely purity is 75.45%.
The enzymolysis degraded sea grass polysaccharide powder that employing the inventive method is prepared and obtained, molecular weight ranges is between 7.2 ~ 122kDa.
The enzymolysis degraded sea grass polysaccharide powder that employing the inventive method is prepared and obtained, measures its anti-oxidant activity, with High Performance Gel Permeation chromatography determining molecular weight.
The present invention has following technique effect:
1., compared with the traditional method such as acid degradation, adopt method of the present invention sea grass polysaccharide action condition of degrading gentle, the reaction times is short.
2. the degradation of polysaccharide molecular weight obtained is lower; Molecular weight ranges is between 7.2 ~ 122kDa.
3., compared with undegradable sea grass polysaccharide, there is better antioxidation activity in vitro.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in further detail.
Fig. 1 is the test result of the DPPH radical scavenging activity of enzymolysis sea grass polysaccharide and the non-degradation of polysaccharide obtained by embodiment of the present invention 1-1.
Fig. 2 is the test result of the ultra-oxygen anion free radical Scavenging activity of enzymolysis sea grass polysaccharide and the non-degradation of polysaccharide obtained by embodiment of the present invention 1-1.
Fig. 3 is the test result of the hydroxyl radical free radical Scavenging activity of enzymolysis sea grass polysaccharide and the non-degradation of polysaccharide obtained by embodiment of the present invention 1-1.
Fig. 4 is the High Performance Gel Permeation color atlas of the enzymolysis sea grass polysaccharide that embodiment of the present invention 1-1 obtains.
Embodiment
The method of embodiment 1-1, enzymic degradation sea grass polysaccharide:
Get the acetic acid-sodium acetate buffer solution (total concn of acetic acid and sodium acetate is 0.1mol/L) that purity is the Enteromorpha Crude polysaccharides pH4.5 of 75.45% and be dissolved into the Crude polysaccharides solution that Enteromorpha Crude polysaccharides concentration is 5mg/mL.
First by 10 × 10
4the saccharifying enzyme (glucoamylase) of U/mL is diluted to 100U/mL by the acetic acid-sodium acetate buffer solution (total concn of acetic acid and sodium acetate is 0.1mol/L) of pH4.5, obtains glucoamylase enzyme solution.Get 710 μ L glucoamylase enzyme solution distilled water and complement to 1mL, glucoamylase enzyme solution after must diluting.
Then in 4mL Crude polysaccharides solution, add the rear glucoamylase enzyme solution of 1ml dilution, thus make the concentration of saccharifying enzyme be 14.2U/mL, the concentration of Enteromorpha Crude polysaccharides is 4mg/mL; Now pH is 4.5, is 2 hours 49 DEG C of reaction times in temperature.
Polysaccharide soln after enzymolysis being boiled 5min, centrifugal removing enzyme (8000r/s, 5min), is then the dialysis tubing dialysis 24h of 500 by gained supernatant liquor molecular weight cut-off, to remove inorganic salt; Obtain dialyzate; Finally dialyzate is carried out lyophilize (i.e. dry 24h in vacuum freeze drying, temperature is-50 DEG C).Obtain the enzymolysis sea grass polysaccharide (that is, obtaining enzymolysis degraded sea grass polysaccharide) that degradation rate is 7.6%.
Remarks illustrate:
1, " enzymolysis polysaccharide ", namely refers to " the enzymolysis sea grass polysaccharide " of final gained.
2, " sea grass polysaccharide " refers to " the Enteromorpha Crude polysaccharides " of non-enzymolysis.
Embodiment 1-2, sea grass polysaccharide is used saccharifying enzyme enzymolysis:
Made the reaction times in embodiment 1-1 into 4 hours by 2 hours, all the other are with embodiment 1-1.
Obtain the enzymolysis sea grass polysaccharide that degradation rate is 11.6%.
Experiment 1,
Adopt document (EspinJC, Soler-RivasC, WichersHJ, etal.Anthocyanin-basednaturalcolorants:anewsourceofantir adicalactivityforfoodstuff [J] .JournalAgriculturalFoodChemistry, 2000,48 (5): 1588-1592.) report method, the enzymolysis sea grass polysaccharide obtained by embodiment 1-1 carries out the test of DPPH radical scavenging activity, and compares with non-degradation of polysaccharide.As seen from Figure 1, sea grass polysaccharide is after enzymolysis, and DPPH radical scavenging activity is significantly improved.When concentration is 5mg/mL, be respectively 21.03 ± 1.67% and 12.2 ± 1.9% by the clearance rate of the sea grass polysaccharide of saccharifying enzyme degraded and the DPPH free radical of non-degradation of polysaccharide.But the activity of the removing DPPH free radical of enzymolysis sea grass polysaccharide is still less than vitamins C.
Experiment 2,
Adopt document (Xu Yan, Qu Tingting. Radix Glycyrrhizae eliminates the in vitro study [J] of oxyradical. food research and development, 2006,27 (8): 63-65.) report method, the enzymolysis sea grass polysaccharide obtained by embodiment 1-1 carries out the test of ultra-oxygen anion free radical Scavenging activity, and compares with non-degradation of polysaccharide.As seen from Figure 2, enzymolysis sea grass polysaccharide to the Scavenging activity of ultra-oxygen anion free radical apparently higher than non-degradation of polysaccharide.When concentration is 5mg/mL, the clearance rate of the ultra-oxygen anion free radical of saccharifying enzyme degradation of polysaccharide and non-degradation of polysaccharide is respectively 56.96 ± 1.67% and 42.09 ± 1.95%.
Experiment 3,
Adopt document (ChenHW, ChenAH, ShaoY, etal.Studiesontheantioxidantcapacityofzincrichexopolysac charideofcordycepsmilitaris [J] .FoodandFermentationIndustries, 2009,35 (6): 54-57.) report method, the enzymolysis sea grass polysaccharide obtained by embodiment 1-1 carries out the test of hydroxyl radical free radical Scavenging activity, and compares with non-degradation of polysaccharide.As seen from Figure 3, enzymolysis sea grass polysaccharide to the Scavenging activity of hydroxyl radical free radical higher than non-degradation of polysaccharide.When concentration is 5mg/mL, the clearance rate of the hydroxyl radical free radical of saccharifying enzyme degradation of polysaccharide and non-degradation of polysaccharide is respectively 44.68 ± 1.72% and 32.37 ± 2.02%.
Experiment 4,
High Performance Gel Permeation Chromatography (HPGPC) is adopted to measure the molecular weight of the enzymolysis sea grass polysaccharide obtained by embodiment 1-1.From the atlas analysis of Fig. 4, this Enteromorpha enzymolysis polysaccharide is heterogencity polysaccharide, and its retention time is from 19.312min to 24.567min, show that its molecular weight ranges is between 7.2 ~ 122kDa.
Comparative example 1-1, made the enzymolysis time in embodiment 1-1 into 0.5 hour, 7 hours respectively by 2 hours, all the other contents are with embodiment 1.
The method of the enzymolysis of gained degraded sea grass polysaccharide according to above-mentioned experiment 1 ~ experiment 3 detected, acquired results is as shown in table 1 below.
Remarks illustrate: following data are result when polysaccharide concentration is 5mg/ml.
Table 1
The method of embodiment 2, enzymic degradation sea grass polysaccharide
Getting purity is that the acetic acid-sodium acetate buffer solution (0.1mol/L) of the Enteromorpha Crude polysaccharides pH4.5 of 75.45% is dissolved into the Crude polysaccharides solution that concentration is 5mg/mL.
First by 10 × 10
4the saccharifying enzyme (glucoamylase) of U/mL is diluted to 100U/mL by the acetic acid-sodium acetate buffer solution (0.1mol/L) of pH4.5, obtains glucoamylase enzyme solution.
The polygalacturonase (enzyme is lived as 30U/mg) of 156mg is settled to 100mL by the acetic acid-sodium acetate buffer solution (0.1mol/L) of pH4.5; Obtain polygalacturonase enzyme liquid.
Then in 4mL Crude polysaccharides solution, add the rear glucoamylase enzyme solution of 1ml dilution, thus make enzyme concn be 14.2U/mL, the concentration of Enteromorpha Crude polysaccharides is 4mg/mL; Now pH is 4.5, is 2 hours 49 DEG C of reaction times in temperature.
Polysaccharide soln after enzymolysis is boiled 5min, centrifugal removing enzyme (8000r/s, 5min), then carries out following hydrolysis again:
That is, thus make the enzyme concentration of polygalacturonase be 7.8% (% by weight) of Enteromorpha Crude polysaccharides polygalacturonase enzyme liquid 1mL is added again; Be 4.5 at pH, temperature is 56 DEG C of reaction 180min, the polysaccharide soln after enzymolysis is boiled 5min, centrifugal removing polygalacturonase (8000r/s, 5min).Then be the dialysis tubing dialysis 24h of 500 by gained supernatant liquor molecular weight cut-off, to remove inorganic salt; Obtain dialyzate; Finally dialyzate is carried out lyophilize (i.e. dry 24h in vacuum freeze drying, temperature is-50 DEG C).Obtain the enzymolysis sea grass polysaccharide (that is, enzymolysis degraded sea grass polysaccharide) that degradation rate is 15.28%.
The method of the enzymolysis of the compound enzyme gained of embodiment 2 degraded sea grass polysaccharide according to above-mentioned experiment 1 ~ experiment 3 detected, acquired results is as shown in table 2 below.
Remarks illustrate: following data are result when polysaccharide concentration is 5mg/ml.
Table 2
| Embodiment 2 | |
| DPPH clearance rate (%) | 66.36±1.39 |
| Ultra-oxygen anion free radical clearance rate (%) | 85.86±1.86 |
| Hydroxyl radical free radical removes (%) | 56.64±1.52 |
Comparative example 2-1, saccharifying enzyme and polygalacturonase in embodiment 2 to be used simultaneously, and the consumption of saccharifying enzyme and polygalacturonase is constant.Specific as follows:
Get 4mL Crude polysaccharides solution, add 0.5ml glucoamylase enzyme solution (142U/ml) and 0.5ml pectinase solution (3.12mg/ml), thus make final saccharifying enzyme concentration be 14.2U/mL, the enzyme concentration of polygalacturonase is 7.8% of Crude polysaccharides total amount.Now pH is 4.5, is 3 hours 50 DEG C of reaction times in temperature.Polysaccharide soln after enzymolysis is boiled 5min, centrifugal removing enzyme (8000r/s, 5min).Then gained supernatant liquor molecular weight cut-off is the dialysis tubing dialysis 24h of 500, to remove inorganic salt; Obtain dialyzate; Finally dialyzate is carried out lyophilize (i.e. dry 24h in vacuum freeze drying).Obtain the enzymolysis sea grass polysaccharide that degradation rate is 12.96%.
The use of comparative example 2-2, cancellation saccharifying enzyme, namely only adopts polygalacturonase to be hydrolyzed; That is, in 4mL Crude polysaccharides solution, add polygalacturonase enzyme liquid 1mL, carry out according to " being again hydrolyzed " described in embodiment 2.
Detected according to the method for above-mentioned experiment 1 ~ experiment 3 by the enzymolysis degraded sea grass polysaccharide of above-mentioned comparative example 2-1 and 2-2 gained, acquired results is as shown in table 3 below.
Remarks illustrate: following data are result when polysaccharide concentration is 5mg/ml.
Table 3
| Comparative example 2-1 | Comparative example 2-2 | |
| DPPH clearance rate (%) | 59.76±2.31 | 53.32±2.05 |
| Ultra-oxygen anion free radical clearance rate (%) | 78.27±2.02 | 74.31±1.72 |
| Hydroxyl radical free radical removes (%) | 51.16±2.72 | 43.86±1.56 |
Comparative example 2-3, make the saccharifying enzyme in embodiment 2 into cellulase, enzyme concn still remains 14.2U/mL; All the other contents are equal to embodiment 2.
Comparative example 2-4, make the saccharifying enzyme in embodiment 2 into α-amylase, enzyme concn still remains 14.2U/mL; All the other contents are equal to embodiment 2.
Comparative example 2-5, make the saccharifying enzyme in embodiment 2 into lipase, enzyme concn still remains 14.2U/mL; All the other contents are equal to embodiment 2.
The method of the enzymolysis of above-mentioned comparative example 2-2 ~ comparative example 2-5 gained degraded sea grass polysaccharide according to above-mentioned experiment 1 ~ experiment 3 detected, acquired results is as shown in table 4 below.
Remarks illustrate: following data are result when polysaccharide concentration is 5mg/ml.
Table 4
| Comparative example 2-3 | Comparative example 2-4 | Comparative example 2-5 | |
| DPPH clearance rate (%) | 14.54±1.63 | 13.12±1.02 | 14.56±1.24 |
| Ultra-oxygen anion free radical clearance rate (%) | 41.86±1.25 | 38.43±1.86 | 39.43±1.67 |
| Hydroxyl radical free radical removes (%) | 34.76±1.66 | 33.67±1.72 | 34.08±1.35 |
Finally, it is also to be noted that what enumerate above is only several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (4)
1. the method for enzymic degradation sea grass polysaccharide, is characterized in that: sea grass polysaccharide is configured to the polysaccharide soln that concentration is 1 ~ 20mg/mL; Then using saccharifying enzyme as lytic enzyme, in polysaccharide soln, saccharifying enzyme is added until the final concentration of saccharifying enzyme is 2 ~ 100U/mL; In pH be 3.5 ~ 5.5, temperature is 40 ~ 60 DEG C of reactions 1 ~ 5 hour, enzymolysis degradation of polysaccharide solution first; After enzymolysis degradation of polysaccharide solution boils 4 ~ 6 minutes first, centrifugal to remove saccharifying enzyme, the supernatant liquor of the rear sea grass polysaccharide of enzymolysis degraded first must be contained.
2. the method for enzymic degradation sea grass polysaccharide according to claim 1, is characterized in that: by being the dialysis tubing dialysis of 500 containing the supernatant liquor molecular weight cut-off of sea grass polysaccharide after enzymolysis degraded first, to remove inorganic salt; Obtain dialyzate; By dialyzate lyophilize, obtain pulverous enzymolysis degraded sea grass polysaccharide.
3. the method for enzymic degradation sea grass polysaccharide according to claim 1, is characterized in that: be again hydrolyzed containing the supernatant liquor of sea grass polysaccharide after enzymolysis degraded first, described hydrolysis again comprises the steps:
In the supernatant liquor containing the rear sea grass polysaccharide of enzymolysis degraded first, add polygalacturonase, the consumption of described polygalacturonase is 1 ~ 10% of sea grass polysaccharide weight; In pH be 3.5 ~ 5.5, temperature is 45 ~ 60 DEG C of reactions 1 ~ 5 hour, enzymolysis degradation of polysaccharide solution again; After enzymolysis degradation of polysaccharide solution boils 4 ~ 6 minutes again, centrifugal to remove polygalacturonase, the supernatant liquor of the rear sea grass polysaccharide of enzymolysis degraded must be contained again.
4. the method for enzymic degradation sea grass polysaccharide according to claim 3, is characterized in that: be the dialysis tubing dialysis of 500 by the supernatant liquor molecular weight cut-off containing again the rear sea grass polysaccharide of enzymolysis degraded, to remove inorganic salt; Obtain dialyzate; By dialyzate lyophilize, obtain pulverous enzymolysis degraded sea grass polysaccharide.
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| CN105399854B (en) * | 2015-12-17 | 2017-07-21 | 浙江工商大学 | Strengthen anti-oxidant and antibacterial activity the method for sea grass polysaccharide simultaneously |
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| CN108239613B (en) * | 2016-12-27 | 2021-07-06 | 中国海洋大学 | Paenibacillus feed, culture medium thereof and application of Paenibacillus feed in preparation of enteromorpha polysaccharide degrading enzyme |
| CN107574197A (en) * | 2017-07-12 | 2018-01-12 | 青岛农业大学 | A kind of biodegradation method of sea grass polysaccharide |
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| CN109924492A (en) * | 2019-04-15 | 2019-06-25 | 桂林理工大学 | A kind of probiotics chewable tablets and preparation method thereof containing intestines sea grass polysaccharide |
| CN114376233A (en) * | 2022-03-23 | 2022-04-22 | 青岛海大生物集团股份有限公司 | Enteromorpha polysaccharide health food with immunity enhancing function and preparation method thereof |
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