CN103947538A - Method for positioning two non-allelic genes for controlling same character of plant - Google Patents
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Abstract
The invention relates to a method for positioning two non-allelic genes for controlling a same character of a plant. The method comprises the following specific steps: (1) a plant with a certain character is selected, and the condition that the character is controlled by two non-allelic genes A and B is assumed, and after mutation of A and B, the character is changed into a mutant character; (2) hybridizing a wild type character with a mutant to obtain F1, wherein a genotype of the F1 is AaBb, the expression of the genotype is the same as that of the wild type, and the F1 has a normal character; (3) the F1 is subjected to selfing to obtain an F2 segregation population, and total nine genotypes exist; (4) the F1 and the mutant are subjected to backcrossing to generate a BC1F1 segregation population, and total four genotypes exist; (5) a plurality of BC1F1 single plants with the normal character are subjected to selfing to obtain respective BC1F2 segregation populations. The method provided by the invention has the beneficial effects that the the advantages of two conventional positioning methods are combined, two non-allelic genes for controlling the same character can be accurately and quickly positioned in the BC1F2 generation, and the method is especially applicable to selfing plants which are not easy for operation in hybridization.
Description
Technical field
The present invention relates to biological technical field, particularly a kind of two non-allelic genes methods of locating the same proterties of plant control.
Background technology
How 2 non-allelic genes controlling same proterties are produced by genome duplication or gene duplication, the most common in allotetraploid plant, as common tobacco, cabbage type rape, durum wheat etc., occasionally in diplont, as paddy rice, arabidopsis etc.Because their biological function is very similar, between exist and have complementary functions, therefore can not cause corresponding recessive phenotype after individual gene sudden change.Molecular labeling is the main and the most reliable means of current gene location, in the location of individual gene and map based cloning, is bringing into play very important effect.But, aspect 2 non-allelic genes of the same proterties of location plant control, at present popular method or positioning result are too coarse, or spended time is oversize.
Aspect 2 non-allelic genes of the same proterties of location plant control, according to used segregation population, mainly contain 2 kinds of methods: one is to utilize F2 or BC1F1 segregation population, assign above-mentioned 2 genes as quantitative trait locus (Quantitative trait loci, QTLs) position, the method due to obtain segregation population very fast (wild type after hybridizing with mutant again selfing once or backcross once with mutant), therefore can obtain fast positioning result, but positioning precision is lower, and can not carry out meticulous location to individual gene.The profit in this way gene of location has 2 rice fertility restorer genes Rf3, Rf (u) and 2 yellowish green phyllopodium of durum wheat because of ygld1, ygld2 etc.; Another kind is to utilize the high method for backcross (Advanced Backcross), first 2 non-allelic genes is separated among the different individual plants that backcrosses, more respectively to its location.The method precision is higher, can be used for the map based cloning of gene, but owing to conventionally could starting location after 3 generations (BC3) that backcross, therefore spended time is longer.The gene that profit is located in this way has three Room siliqua gene mc1 of 1 cabbage type rape male sterile gene ms2 and 1 Indian mustard etc.
Summary of the invention
For the defect existing in prior art, the object of this invention is to provide a kind of two non-allelic genes methods of locating the same proterties of plant control.
The present invention adopts following technical scheme:
Two nonallelic methods of locating the same proterties of plant control, concrete step is as follows:
1) choose the plant with a certain proterties, suppose that this proterties is controlled by 2 non-allelic genes A and B, after A and B sudden change, this proterties becomes mutant character, the genotype of wild type is AABB so, show as normal proterties, and the genotype of mutant is aabb, shows as mutant character;
2) wild type and mutant hybridization are obtained to F1, the genotype of F1 is AaBb, shows same wild type, is normal proterties;
3) after F1 selfing, obtain F2 segregation population, have 9 kinds of frequency of genotypes AA BB, AABb, AAbb, AaBB, AaBb, Aabb, aaBB, aaBb, aabb, wherein aabb accounts for 1/16, so the ratio that separates of the normal proterties of F2 colony and mutant character shows as 15:1;
4) F1 is backcrossed with mutant, the BC1F1 segregation population of generation has 4 kinds of genotype AaBb, Aabb, aaBb, aabb, respectively accounts for 1/4, so the normal proterties of BC1F1 colony separates with mutant character than showing as 3:1;
5) get the BC1F1 individual plant selfing of some normal proterties, obtain BC1F2 segregation population separately;
The individual plant genotype of the normal proterties of BC1F1 due to 1/3 is AaBb, and the BC1F2 segregation population that its selfing produces is so identical with F1, has 9 kinds of genotype, and the ratio that separates of normal proterties and mutant character shows as 15:1;
Separately having 1/3 BC1F1 individual plant genotype is Aabb, and the BC1F2 segregation population that its selfing produces so has 3 kinds of frequency of genotypes AA bb, Aabb, aabb, and wherein aabb accounts for 1/4, so the ratio that separates of normal proterties and mutant character shows as 3:1; Because separating of normal proterties and mutant character is only relevant to the genotypic separation of A, containing A is normal proterties, and not containing A is mutant character, therefore this BC1F2 colony is the colony about A Gene Isolation, A gene is positioned with molecular labeling;
The BC1F1 individual plant genotype of residue 1/3 is aaBb,, the BC1F2 segregation population that its selfing produces so has 3 kinds of genotype aaBB, aaBb, aabb, and wherein aabb accounts for 1/4, so the ratio that separates of normal proterties and mutant character shows as 3:1; Because separating of normal proterties and mutant character is only relevant to the genotypic separation of B, containing B is normal proterties, is not mutant character containing B, therefore this BC1F2 colony is the colony about B Gene Isolation, can position B gene with molecular labeling;
Two non-allelic genes that utilize the above-mentioned common tobacco stem stalk of method positioning control color, concrete step is as follows:
1) the stem culm base of normal Zhongyan-100 is green, the stem culm base of mutant is white in color, with mutant and stem stalk be the green large gold dollar hybridization of tobacco bred safflower, obtain hybrid F1, F1 selfing obtains F2 segregation population, and F1 backcrosses and obtains BC1F1 segregation population with the large gold dollar of safflower simultaneously.Stem stalk green and white individual plant number in F2 and BC1F1 colony have been carried out to statistics and Chi-square Test, find green white separation than meeting respectively 15:1 and 3:1, this shows that white stem stalk proterties is subject to 2 recessive gene controls, we are respectively by its called after c and d, and the dominant gene of their correspondences is respectively C and D so;
2) in the BC1F1 segregation population from step 1, chosen the individual plant selfing of 9 strain stem stalk greens, obtained BC1F2 segregation population separately, be numbered respectively No. 1-9; Stem stalk green and white individual plant number in these colonies have been carried out to statistics and Chi-square Test, the green white separation ratio of finding 4 colonies of No. 1-4 meets 15:1, and the green white separation ratio of 5 colonies of No. 5-9 meets 3:1 in addition, this genotype that shows previous generation BC1F1 individual plant corresponding to the 5th colony is Ccdd or ccDd, can be used for c gene or d gene to position;
3) adopt tobacco SSR molecular labeling to carry out gene location
According to common tobacco SSR mark linkage map M, the genomic DNA of choosing 1376 pairs of SSR primer pair mutant and the large gold dollar of safflower carries out PCR, about common tobacco SSR mark linkage map M and 1376 couples of SSR primer bibliography: Bindler, G., Plieske, J., Bakaher, N., Gunduz, I., Ivanov, N., Van der Hoeven, R., Ganal, M.and Donini, P. (2011) A high density genetic map of tobacco (Nicotiana tabacum L.) obtained from large scale microsatellite marker development.Theoretical and Applied Genetics, 123, 219-230.
PCR product separates through 6% polyacrylamide gel electrophoresis, obtain altogether 183 pairs of primers that have polymorphism between mutant and the large gold dollar of safflower, increase and electrophoresis detection with 10 green stem stalk individual plants of No. 5 BC1F2 colony of these primer pairs and the genomic DNA of 10 white stem stalk individual plants, find that primer PT61414 in the 5th linkage group is at 10 white stem stalk individual plants, be in recessive individual plant, to only have 2 individual plants to exchange, and in 10 green stem stalk individual plants, do not observe exchange, we have further detected remaining 41 recessive individual plants in No. 5 colony with this primer, finally from 51 recessive individual plants, find altogether 6 exchange individual plants, this shows that the controlling gene in this primer and No. 5 colony is chain, we suppose that this gene is c,
4) adopt the method identical with step 3, extract the genomic DNA of each 10 green stem stalk individual plants and 10 white stem stalk individual plants in 6-9 BC1F2 colony, detect with PT61414, find that the controlling gene in this primer and the 6th, No. 7 colonies is chain, but not with the 8th, No. 9 colonies in controlling gene chain, the genotype of previous generation BC1F1 individual plant corresponding to such 5-7 colony is Ccdd, and the genotype of previous generation BC1F1 individual plant corresponding to the 8th, No. 9 colonies should be ccDd;
Increase and electrophoresis detection with 11 green stem stalk individual plants of No. 8 colony of 183 pairs of polymorphism SSR primer pairs and the genomic DNA of 10 white stem stalk individual plants, find that primer PT53716 in the 24th linkage group is at 10 white stem stalk individual plants, only have 3 individual plants to exchange, and in 11 green stem stalk individual plants, do not observe exchange, we further detect remaining 38 recessive individual plants in Liao Gai colony with this primer, finally from 48 recessive individual plants, find altogether 4 exchange individual plants, this shows this primer and d gene linkage; Like this, we have located respectively 2 non-allelic genes controlling common tobacco white stem stalk proterties, and they lay respectively in common tobacco the 5th and 24SSR mark linkage group.
Two non-allelic genes methods of the same proterties of location plant control as above, the method is controlled two non-allelic genes of same proterties for locating allotetraploid plant.
Above-mentioned allotetraploid plant is common tobacco, cabbage type rape, durum wheat.
The invention has the beneficial effects as follows:
This method combines the advantage of 2 kinds of traditional localization methods, can be not only accurate but also in BC1F2 generation, 2 non-allelic genes controlling same proterties are positioned rapidly, be specially adapted to the not easy-operating selfing plant of hybridization.
Brief description of the drawings
The present invention has following accompanying drawing:
Fig. 1 is 10 green stem stalk individual plants of No. 5 BC1F2 colony and the gel electrophoresis figure of the genomic DNA of 10 white stem stalk individual plants after tobacco SSR primer PT61414 amplification in embodiment 2; First band of left side is the large gold dollar of safflower, and second band is mutant, and 3-12 is 10 green stem stalk individual plants, and 13-22 is 10 white stem stalk individual plants; Asterisk represents to exchange individual plant;
Fig. 2 is the gel electrophoresis figure of tobacco SSR primer PT53716 after the genomic DNA of 11 of No. 8 colony green stem stalk individual plants and 10 white stem stalk individual plants is increased in embodiment 2; First band of left side is the large gold dollar of safflower, and second band is mutant, and 3-13 is 11 green stem stalk individual plants, and 14-23 is 10 white stem stalk individual plants; Asterisk represents to exchange individual plant.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail.
Embodiment 1
Two nonallelic methods of locating the same proterties of plant control, concrete step is as follows:
1) choose the plant with a certain proterties, suppose that this proterties is controlled by 2 non-allelic genes A and B, after A and B sudden change, this proterties becomes mutant character, the genotype of wild type is AABB so, show as normal proterties, and the genotype of mutant is aabb, shows as mutant character;
2) wild type and mutant hybridization are obtained to F1, the genotype of F1 is AaBb, shows same wild type, is normal proterties;
3) after F1 selfing, obtain F2 segregation population, have 9 kinds of frequency of genotypes AA BB, AABb, AAbb, AaBB, AaBb, Aabb, aaBB, aaBb, aabb, wherein aabb accounts for 1/16, so the ratio that separates of the normal proterties of F2 colony and mutant character shows as 15:1;
4) F1 is backcrossed with mutant, the BC1F1 segregation population of generation has 4 kinds of genotype AaBb, Aabb, aaBb, aabb, respectively accounts for 1/4, so the normal proterties of BC1F1 colony separates with mutant character than showing as 3:1;
5) get the BC1F1 individual plant selfing of some normal proterties, obtain BC1F2 segregation population separately;
The individual plant genotype of the normal proterties of BC1F1 due to 1/3 is AaBb, and the BC1F2 segregation population that its selfing produces is so identical with F1, has 9 kinds of genotype, and the ratio that separates of normal proterties and mutant character shows as 15:1;
Separately having 1/3 BC1F1 individual plant genotype is Aabb, and the BC1F2 segregation population that its selfing produces so has 3 kinds of frequency of genotypes AA bb, Aabb, aabb, and wherein aabb accounts for 1/4, so the ratio that separates of normal proterties and mutant character shows as 3:1; Because separating of normal proterties and mutant character is only relevant to the genotypic separation of A, containing A is normal proterties, and not containing A is mutant character, therefore this BC1F2 colony is the colony about A Gene Isolation, A gene is positioned with molecular labeling;
The BC1F1 individual plant genotype of residue 1/3 is aaBb,, the BC1F2 segregation population that its selfing produces so has 3 kinds of genotype aaBB, aaBb, aabb, and wherein aabb accounts for 1/4, so the ratio that separates of normal proterties and mutant character shows as 3:1; Because separating of normal proterties and mutant character is only relevant to the genotypic separation of B, containing B is normal proterties, is not mutant character containing B, therefore this BC1F2 colony is the colony about B Gene Isolation, can position B gene with molecular labeling;
Embodiment 2
Two non-allelic genes that utilize the above-mentioned common tobacco stem stalk of method positioning control color, concrete step is as follows:
1) the stem culm base of normal Zhongyan-100 is green, the stem culm base of mutant is white in color, with mutant and stem stalk be the green large gold dollar hybridization of tobacco bred safflower, obtain hybrid F1, F1 selfing obtains F2 segregation population, and F1 backcrosses and obtains BC1F1 segregation population with the large gold dollar of safflower simultaneously.Stem stalk green and white individual plant number in F2 and BC1F1 colony have been carried out to statistics and Chi-square Test, find green white separation than meeting respectively 15:1 and 3:1, this shows that white stem stalk proterties is subject to 2 recessive gene controls, we are respectively by its called after c and d, and the dominant gene of their correspondences is respectively C and D so;
The phenotype of F2 and BC1F1 segregation population statistics in table 1 step 1
| Segregation population | Total strain number | The green strain number of stem stalk | Stem stalk white strain number | Separate ratio | Chi-square value |
| F2 | 285 | 273 | 12 | 22.75:1 | 2.023 |
| BC1F1 | 134 | 106 | 28 | 3.79:1 | 1.204 |
2) in the BC1F1 segregation population from step 1, chosen the individual plant selfing of 9 strain stem stalk greens, obtained BC1F2 segregation population separately, be numbered respectively No. 1-9; Stem stalk green and white individual plant number in these colonies have been carried out to statistics and Chi-square Test, the green white separation ratio of finding 4 colonies of No. 1-4 meets 15:1, and the green white separation ratio of 5 colonies of No. 5-9 meets 3:1 in addition, this genotype that shows previous generation BC1F1 individual plant corresponding to the 5th colony is Ccdd or ccDd, can be used for c gene or d gene to position;
Individual plant selfing statistics and the Chi-square Test of 9 strain stem stalk greens in table 2BC1F1 segregation population,
| Numbering | Total strain number | The green strain number of stem stalk | Stem stalk white strain number | Separate ratio | Chi-square value |
| 1 | 93 | 88 | 5 | 17.60:1 | 0.121 |
| 2 | 94 | 89 | 5 | 17.80:1 | 0.139 |
| 3 | 134 | 128 | 6 | 21.33:1 | 0.718 |
| 4 | 91 | 87 | 4 | 21.75:1 | 0.534 |
| 5 | 241 | 190 | 51 | 3.73:1 | 1.893 |
| 6 | 130 | 101 | 29 | 3.48:1 | 0.503 |
| 7 | 91 | 73 | 18 | 4.06:1 | 1.322 |
| 8 | 235 | 187 | 48 | 3.90:1 | 2.623 |
| 9 | 97 | 80 | 17 | 4.71:1 | 2.890 |
3) adopt tobacco SSR molecular labeling to carry out gene location
According to common tobacco SSR mark linkage map M, the genomic DNA of choosing 1376 pairs of SSR primer pair mutant and the large gold dollar of safflower carries out PCR, PCR product separates through 6% polyacrylamide gel electrophoresis, obtain altogether 183 pairs of primers that have polymorphism between mutant and the large gold dollar of safflower, increase and electrophoresis detection with 10 green stem stalk individual plants of No. 5 BC1F2 colony of these primer pairs and the genomic DNA of 10 white stem stalk individual plants, find that primer PT61414 in the 5th linkage group is at 10 white stem stalk individual plants, be in recessive individual plant, to only have 2 individual plants to exchange, and in 10 green stem stalk individual plants, do not observe exchange, we have further detected remaining 41 recessive individual plants in No. 5 colony with this primer, finally from 51 recessive individual plants, find altogether 6 exchange individual plants, this shows that the controlling gene in this primer and No. 5 colony is chain, we suppose that this gene is c,
4) adopt the method identical with step 3, extract the genomic DNA of each 10 green stem stalk individual plants and 10 white stem stalk individual plants in 6-9 BC1F2 colony, detect with PT61414, find that the controlling gene in this primer and the 6th, No. 7 colonies is chain, but not with the 8th, No. 9 colonies in controlling gene chain, the genotype of previous generation BC1F1 individual plant corresponding to such 5-7 colony is Ccdd, and the genotype of previous generation BC1F1 individual plant corresponding to the 8th, No. 9 colonies should be ccDd;
Increase in 10 green stem stalk individual plants of 6-9 colony and 10 the white stem stalk individual plants statistics of banding pattern of table 3 tobacco SSR primer PT61414
Increase and electrophoresis detection with 11 green stem stalk individual plants of No. 8 colony of 183 pairs of polymorphism SSR primer pairs and the genomic DNA of 10 white stem stalk individual plants, find that primer PT53716 in the 24th linkage group is at 10 white stem stalk individual plants, only have 3 individual plants to exchange, and in 11 green stem stalk individual plants, do not observe exchange, we further detect remaining 38 recessive individual plants in Liao Gai colony with this primer, finally from 48 recessive individual plants, find altogether 4 exchange individual plants, this shows this primer and d gene linkage; Like this, we have located respectively 2 non-allelic genes controlling common tobacco white stem stalk proterties, and they lay respectively in common tobacco the 5th and 24SSR mark linkage group.
Claims (5)
1. two nonallelic methods of locating the same proterties of plant control, is characterized in that concrete step is as follows:
1) choose the plant with a certain proterties, suppose that this proterties is controlled by 2 non-allelic genes A and B, after A and B sudden change, this proterties becomes mutant character, the genotype of wild type is AABB so, show as normal proterties, and the genotype of mutant is aabb, shows as mutant character;
2) wild type and mutant hybridization are obtained to F1, the genotype of F1 is AaBb, shows same wild type, is normal proterties;
3) after F1 selfing, obtain F2 segregation population, have 9 kinds of frequency of genotypes AA BB, AABb, AAbb, AaBB, AaBb, Aabb, aaBB, aaBb, aabb, wherein aabb accounts for 1/16, so the ratio that separates of the normal proterties of F2 colony and mutant character shows as 15:1;
4) F1 is backcrossed with mutant, the BC1F1 segregation population of generation has 4 kinds of genotype AaBb, Aabb, aaBb, aabb, respectively accounts for 1/4, so the normal proterties of BC1F1 colony separates with mutant character than showing as 3:1;
5) get the BC1F1 individual plant selfing of some normal proterties, obtain BC1F2 segregation population separately;
The individual plant genotype of the normal proterties of BC1F1 due to 1/3 is AaBb, and the BC1F2 segregation population that its selfing produces is so identical with F1, has 9 kinds of genotype, and the ratio that separates of normal proterties and mutant character shows as 15:1;
Separately having the green individual plant genotype of 1/3 BC1F1 is Aabb, and the BC1F2 segregation population that its selfing produces so has 3 kinds of frequency of genotypes AA bb, Aabb, aabb, and wherein aabb accounts for 1/4, so the ratio that separates of normal proterties and mutant character shows as 3:1; Because separating of normal proterties and mutant character is only relevant to the genotypic separation of A, containing A is normal proterties, and not containing A is mutant character, therefore this BC1F2 colony is the colony about A Gene Isolation, A gene is positioned with molecular labeling;
The green individual plant genotype of BC1F1 of residue 1/3 is aaBb,, the BC1F2 segregation population that its selfing produces so has 3 kinds of genotype aaBB, aaBb, aabb, and wherein aabb accounts for 1/4, so the ratio that separates of normal proterties and mutant character shows as 3:1; Because separating of normal proterties and mutant character is only relevant to the genotypic separation of B, containing B is normal proterties, is not mutant character containing B, therefore this BC1F2 colony is the colony about B Gene Isolation, can position B gene with molecular labeling.
2. two non-allelic genes that utilize the common tobacco stem stalk of the method positioning control color described in claim 1, is characterized in that concrete step is as follows:
1) the stem culm base of normal Zhongyan-100 is green, the stem culm base of mutant is white in color, with mutant and stem stalk be the green large gold dollar hybridization of tobacco bred safflower, obtain hybrid F1, F1 selfing obtains F2 segregation population, and F1 backcrosses and obtains BC1F1 segregation population with the large gold dollar of safflower simultaneously.Stem stalk green and white individual plant number in F2 and BC1F1 colony have been carried out to statistics and Chi-square Test, find green white separation than meeting respectively 15:1 and 3:1, this shows that white stem stalk proterties is subject to 2 recessive gene controls, we are respectively by its called after c and d, and the dominant gene of their correspondences is respectively C and D so;
2) in the BC1F1 segregation population from step 1, chosen the individual plant selfing of 9 strain stem stalk greens, obtained BC1F2 segregation population separately, be numbered respectively No. 1-9; Stem stalk green and white individual plant number in these colonies have been carried out to statistics and Chi-square Test, the green white separation ratio of finding 4 colonies of No. 1-4 meets 15:1, and the green white separation ratio of 5 colonies of No. 5-9 meets 3:1 in addition, this genotype that shows previous generation BC1F1 individual plant corresponding to the 5th colony is Ccdd or ccDd, can be used for c gene or d gene to position;
3) adopt tobacco SSR molecular labeling to carry out gene location
According to common tobacco SSR mark linkage map M, the genomic DNA of choosing 1376 pairs of SSR primer pair mutant and the large gold dollar of safflower carries out PCR, PCR product separates through 6% polyacrylamide gel electrophoresis, obtain altogether 183 pairs of primers that have polymorphism between mutant and the large gold dollar of safflower, increase and electrophoresis detection with 10 green stem stalk individual plants of No. 5 BC1F2 colony of these primer pairs and the genomic DNA of 10 white stem stalk individual plants, find that primer PT61414 in the 5th linkage group is at 10 white stem stalk individual plants, be in recessive individual plant, to only have 2 individual plants to exchange, and in 10 green stem stalk individual plants, do not observe exchange, we have further detected remaining 41 recessive individual plants in No. 5 colony with this primer, finally from 51 recessive individual plants, find altogether 6 exchange individual plants, this shows that the controlling gene in this primer and No. 5 colony is chain, we suppose that this gene is c,
4) adopt the method identical with step 3, extract the genomic DNA of each 10 green stem stalk individual plants and 10 white stem stalk individual plants in 6-9 BC1F2 colony, detect with PT61414, find that the controlling gene in this primer and the 6th, No. 7 colonies is chain, but not with the 8th, No. 9 colonies in controlling gene chain, the genotype of previous generation BC1F1 individual plant corresponding to such 5-7 colony is Ccdd, and the genotype of previous generation BC1F1 individual plant corresponding to the 8th, No. 9 colonies should be ccDd;
Increase and electrophoresis detection with 11 green stem stalk individual plants of No. 8 colony of 183 pairs of polymorphism SSR primer pairs and the genomic DNA of 10 white stem stalk individual plants, find that primer PT53716 in the 24th linkage group is at 10 white stem stalk individual plants, only have 3 individual plants to exchange, and in 11 green stem stalk individual plants, do not observe exchange, we further detect remaining 38 recessive individual plants in Liao Gai colony with this primer, finally from 48 recessive individual plants, find altogether 4 exchange individual plants, this shows this primer and d gene linkage; Like this, we have located respectively 2 non-allelic genes controlling common tobacco white stem stalk proterties, and they lay respectively in common tobacco the 5th and 24SSR mark linkage group.
3. two non-allelic genes methods of the same proterties of location according to claim 1 plant control, is characterized in that the method controls two non-allelic genes of same proterties for locating plant.
4. two non-allelic genes methods of the same proterties of location plant control according to claim 3, is characterized in that described plant is allotetraploid plant.
5. two non-allelic genes methods of the same proterties of location plant control according to claim 4, is characterized in that described allotetraploid plant is common tobacco, cabbage type rape, durum wheat.
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| CN108707690A (en) * | 2018-06-07 | 2018-10-26 | 中国农业科学院烟草研究所 | The molecular labeling isolated with burley tobaccos control gene and its application |
| CN108707690B (en) * | 2018-06-07 | 2022-02-01 | 中国农业科学院烟草研究所 | Molecular marker coseparated with burley tobacco control gene and application thereof |
| CN108588263B (en) * | 2018-06-07 | 2022-02-01 | 中国农业科学院烟草研究所 | Method for positioning and cloning double recessive genes for controlling same character in plant |
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