CN103933066A - Method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide - Google Patents
Method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide Download PDFInfo
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- CN103933066A CN103933066A CN201410088558.XA CN201410088558A CN103933066A CN 103933066 A CN103933066 A CN 103933066A CN 201410088558 A CN201410088558 A CN 201410088558A CN 103933066 A CN103933066 A CN 103933066A
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- cefalexin
- cupric oxide
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- cephalexin
- copper oxide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention specifically relates to a method for improving antibacterial activity of cephalexin through synergy of nanometer copper oxide, which belongs to the technical field of biological medicine. According to the method, a nanometer copper oxide inorganic nano-material is added into cephalexin so as to improve antibacterial activity on Escherichia coli. According to results of antibacterial experiments, it is found that nanometer copper oxide has a good synergistic effect on antibacterial activity of cephalexin; drug effects produced in combined usage of nanometer copper oxide and cephalexin are far better than drug effects produced in separate usage of nanometer copper oxide and cephalexin. The method has the advantages of easy availability of raw materials, low cost and simple operation.
Description
Technical field
The invention belongs to biological medicine technology field, be specifically related to the synergisting method of a kind of nano cupric oxide to cefalexin bacteriostasis property.
Background technology
For a long time, people are seeking to have the antibiotic medicine of higher curative effect always.Mainly contain at present Liang Tiao road and improve effect of medicine: a kind of method is the new powerful antibiotic of exploitation, but because bringing out pathogenic bacteria, extensive use meeting produces drug resistance, thereby inevitably cause the reduction of drug effect, another kind method is the using dosage that increases medicine, declines with the curative effect that makes up medicine.But, due to the terminal that doctor cannot judge medication accurately, in the process of medication treatment, always take excessive principle, and this will bring on physiology with patient, brings psychologically and economically multiple burden.In order to address these problems, nanotechnology has been applied to drug research field.Metal nanoparticle is owing to having antibacterial activity, people's its a kind of method as effective solution drug resistance that begins one's study.But nano-metal particle itself has certain toxicity to human body cell, this has limited its application to a certain extent.
Summary of the invention
The object of the invention is to propose the synergetic method of a kind of nano cupric oxide to cefalexin anti-microbial property.The present invention by nano cupric oxide and this broad ectrum antibiotic of cefalexin under variable concentrations combined effect in escherichia coli, when significantly reducing both using dosages, the fungistatic effect while obtaining using separately far above both.
the technical scheme that the present invention provides:
Adopt synergy thinking, found out the pairing between suitable antibiotic and nano cupric oxide, it is characterized in that, proposed the synergisting method of a kind of nano cupric oxide to cefalexin anti-microbial property, comprised the following steps:
(1) setting nano oxidized copper solution is 3.125:1-12.5:1 with cefalexin quality than scope, nano cupric oxide granule is scattered in and in sterilized water, is made into certain density solution, cefalexin powder is dissolved in to sterilized water wiring solution-forming, nano oxidized copper solution is mixed with cefalexin solution, at 30 minutes mix homogeneously of ultrasonic lower effect.
(2) utilize the cefalexin and the nano cupric oxide mixed solution that obtain to produce Synergistic performance to escherichia coli.
Contrast verification effect, concrete grammar is as follows:
Escherichia coli (
bH21) join in the LB fluid medium preparing, at 37 DEG C, cultivate 24 hours so that its activity recovery.The Escherichia coli bacteria liquid that cultivation is obtained dilutes with LB fluid medium, makes its concentration reach 2.5 × 10
2cFU.The Escherichia coli bacteria liquid of getting after 2ml dilution adds in the cefalexin solution of variable concentrations, nano oxidized copper solution and cefalexin and nano cupric oxide mixed solution.Mixed solution is placed in 37 DEG C of isothermal vibration ponds and acts on 30 minutes, from every kind of solution, get 100 μ l and be uniformly coated on LB solid medium flat board, at 37 DEG C, cultivate observed result 24 hours.
The present invention proposes nano cupric oxide to be applied in the field of associating antibacterial first.The beneficial effect that technical solution of the present invention is brought: the present invention utilizes nano cupric oxide to carry out Synergistic to the commercially available medicament of routine, for effectively improving the curative effect of medicine, particularly how be optimized improvement a kind of brand-new probability is provided producing the medicine of bacterial drug resistance.Only adding on the basis of a small amount of inorganic nano material nano cupric oxide, greatly reduce the using dosage of original medicament, keep the original higher fungistatic effect that even reaches, the far super drug effect that both obtain while using separately simultaneously.This provides new experience for developing new antibacterial.
Brief description of the drawings
Fig. 1 is transmission electron microscope (TEM) photo of embodiment 1 nano cupric oxide granule used.
Fig. 2 is the cefalexin solution of variable concentrations in embodiment 1,2,3, nano oxidized copper solution and cefalexin and the antibacterial actual effect figure of nano cupric oxide mixed solution.Wherein:
A-b:4,8mg l
-1cefalexin,
E-g:25,50,200mg l
-1nano cupric oxide,
C-d, h:8 mg l
-1cefalexin+50mg l
-1nano cupric oxide, 8mg l
-1cefalexin+25mg l
-1nano cupric oxide, 4 mg l
-1cefalexin+50mg l
-1nano cupric oxide.
Fig. 3 is the cefalexin solution of variable concentrations in embodiment 1,2,3, nano oxidized copper solution and cefalexin and nano cupric oxide mixed solution the anti-bacterial result cartogram.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is further illustrated.The all raw materials that participate in embodiment in reaction system are commercially available.Cefalexin is purchased from Ming Rui bio tech ltd, Shanghai (article No. MR10808, CAS 15686-71-2), purity 98%.Nano cupric oxide is purchased from Aladdin reagent (Shanghai) Co., Ltd. (article No. C112567, CAS 1317-38-0), purity 99.5%, preparation LB culture medium sodium chloride (AR used, Shanghai examination, article No. 10019318, CAS 7647-14-5), tryptone (BR, Shanghai examination, article No. 69024138, CAS 91079-40-2), agar powder (BR, Shanghai examination, article No. 10000561, CAS 9002-18-0) be all purchased from Chemical Reagent Co., Ltd., Sinopharm Group.Yeast extract powder (article No. YSYL004, CAS 8013-01-2) is purchased from the biological company limited of Zhengzhou subepoch, and test is Tongji University's self-control with deionized water.Above reagent is not purified direct use all.
embodiment 1
The first step, is scattered in commercially available nano cupric oxide granule in sterilized water, to be made into that concentration is respectively 25,50,200mg l
-1solution, be placed in ultrasonic lower effect and it fully disperseed in 30 minutes, cefalexin powder is dissolved in to sterilized water wiring solution-forming, concentration is respectively 4,8mg l
-1; Be 8 mg l by concentration
-1cefalexin and concentration be 50mg l
-1nano cupric oxide mix, within 30 minutes, make its effect fully in ultrasonic lower effect.
Second step, escherichia coli (
bH21) join in the LB fluid medium preparing, at 37 DEG C, cultivate 24 hours so that its activity recovery.The Escherichia coli bacteria liquid that cultivation is obtained dilutes by LB culture medium, makes its its concentration reach 2.5 × 10
2cFU.Get that Escherichia coli bacteria liquid that 2ml diluted adds 4,8mg l
-1cefalexin, 25,50,200mg l
-1nano cupric oxide, 8 mg l
-1cefalexin+50mg l
-1in nano cupric oxide mixed solution.Mixed solution is put into 37 DEG C of isothermal vibration ponds and act on 30 minutes, from every kind of solution, get 100 μ l and be uniformly coated on LB solid medium flat board, at 37 DEG C, cultivate observed result 24 hours.
From
fig. 2 and Fig. 3in we can see that nano cupric oxide and cefalexin mixed solution (both mass ratioes are 6.25:1) obtained very good collaborative fungistatic effect (c, bacteriostasis rate has reached 95%), super independent use cefalexin (b far away, bacteriostasis rate 30%) and the drug effect that reaches when nano cupric oxide (f, bacteriostasis rate 28%).
embodiment 2
The first step, is scattered in commercially available nano cupric oxide granule in sterilized water, to be made into that concentration is respectively 25,50,200mg l
-1solution, be placed in ultrasonic lower effect and it fully disperseed in 30 minutes, cefalexin powder is dissolved in to sterilized water wiring solution-forming (B), concentration is respectively 4,8mg l
-1; Be 8 mg l by concentration
-1cefalexin and concentration be 25mg l
-1nano cupric oxide mix, within 30 minutes, make its effect fully in ultrasonic lower effect.
Second step, escherichia coli (
bH21) join in the LB fluid medium preparing, at 37 DEG C, cultivate 24 hours so that its activity recovery.The Escherichia coli bacteria liquid that cultivation is obtained dilutes by LB culture medium, makes its its concentration reach 2.5 × 10
2cFU.Get that Escherichia coli bacteria liquid that 2ml diluted adds 4,8mg l
-1cefalexin, 25,50,200mg l
-1nano cupric oxide, 8 mg l
-1cefalexin+25mg l
-1in nano cupric oxide mixed solution.Mixed solution is put into 37 DEG C of isothermal vibration ponds and act on 30 minutes, from every kind of solution, get 100 μ l and be uniformly coated on LB solid medium flat board, at 37 DEG C, cultivate observed result 24 hours.
From
fig. 2 and Fig. 3in we can see that nano cupric oxide and cefalexin mixed solution obtained very good collaborative fungistatic effect (both mass ratioes are 3.125:1) and obtained very good collaborative fungistatic effect (d, bacteriostasis rate has reached 96%), super independent use cefalexin (b far away, bacteriostasis rate 30%) and the drug effect that reaches when nano cupric oxide (e, bacteriostasis rate 23%).
embodiment 3
The first step, is scattered in commercially available nano cupric oxide granule in sterilized water, to be made into that concentration is respectively 25,50,200mg l
-1solution, be placed in ultrasonic lower effect and it fully disperseed in 30 minutes, cefalexin powder is dissolved in to sterilized water wiring solution-forming (B), concentration is respectively 4,8mg l
-1; Be 4 mg l by concentration
-1cefalexin and concentration be 50mg l
-1nano cupric oxide mix, within 30 minutes, make its effect fully in ultrasonic lower effect.
Second step, escherichia coli (
bH21) join in the LB fluid medium preparing, at 37 DEG C, cultivate 24 hours so that its activity recovery.The Escherichia coli bacteria liquid that cultivation is obtained dilutes by LB culture medium, makes its its concentration reach 2.5 × 10
2cFU.Get that Escherichia coli bacteria liquid that 2ml diluted adds 4,8mg l
-1cefalexin, 25,50,200mg l
-1nano cupric oxide, 4 mg l
-1cefalexin+50mg l
-1in nano cupric oxide mixed solution.Mixed solution is put into 37 DEG C of isothermal vibration ponds and act on 30 minutes, from every kind of solution, get 100 μ l and be uniformly coated on LB solid medium flat board, at 37 DEG C, cultivate observed result 24 hours.
From
fig. 2 and Fig. 3in we can see that nano cupric oxide and cefalexin mixed solution (both mass ratioes are 12.5:1) obtained very good collaborative fungistatic effect and obtained very good collaborative fungistatic effect (h, bacteriostasis rate has reached 98%), super independent use cefalexin (a far away, bacteriostasis rate 11%) and the drug effect that reaches when nano cupric oxide (f, bacteriostasis rate 28%).
The bacteriostasis rate that this embodiment reaches is the highest, is most preferred embodiment.
The above-mentioned description to embodiment is can understand and apply the invention for ease of those skilled in the art.Person skilled in the art obviously can easily make various amendments to these embodiment, and General Principle described herein is applied in other embodiment and needn't passes through performing creative labour.Therefore, the invention is not restricted to the embodiment here, the amendment of making in the situation that not departing from category of the present invention is all within protection scope of the present invention.
Claims (1)
1. nano cupric oxide, to a cefalexin bacteriostasis property synergisting method, is characterized in that, comprises the following steps:
(1) setting nano oxidized copper solution is 3.125:1-12.5:1 with cefalexin quality than scope, nano cupric oxide granule is scattered in and in sterilized water, is made into certain density solution, cefalexin powder is dissolved in to sterilized water wiring solution-forming, nano oxidized copper solution is mixed with cefalexin solution, at 30 minutes mix homogeneously of ultrasonic lower effect;
(2) utilize the cefalexin and the nano cupric oxide mixed solution that obtain to produce Synergistic performance to escherichia coli.
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| CN103933066B CN103933066B (en) | 2017-05-24 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758314A (en) * | 2015-03-10 | 2015-07-08 | 同济大学 | Synergism method of antibacterial performance of ceftazidime by nano silver phosphate |
| CN105271359A (en) * | 2015-11-06 | 2016-01-27 | 武汉工程大学 | Nanometer copper oxide and preparation method and application thereof |
| CN110241174A (en) * | 2019-06-19 | 2019-09-17 | 同济大学 | The preparation method of nano magnesia raising kanamycins antibiotic property |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001000021A1 (en) * | 1999-06-25 | 2001-01-04 | Arch Chemicals, Inc. | Pyrithione biocides enhanced by silver, copper, or zinc ions |
| CN1850113A (en) * | 2006-03-02 | 2006-10-25 | 同济大学 | Amoxicillin antibacterial agent containing nano silver, and its preparing method and use |
| US20090016990A1 (en) * | 2007-01-24 | 2009-01-15 | Alberte Randall S | Antimicrobial Compositions |
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2014
- 2014-03-12 CN CN201410088558.XA patent/CN103933066B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2001000021A1 (en) * | 1999-06-25 | 2001-01-04 | Arch Chemicals, Inc. | Pyrithione biocides enhanced by silver, copper, or zinc ions |
| CN1850113A (en) * | 2006-03-02 | 2006-10-25 | 同济大学 | Amoxicillin antibacterial agent containing nano silver, and its preparing method and use |
| US20090016990A1 (en) * | 2007-01-24 | 2009-01-15 | Alberte Randall S | Antimicrobial Compositions |
Non-Patent Citations (2)
| Title |
|---|
| GUOGANG REN ET AL: "Characterisation of copper oxide nanoparticles for antimicrobial applications", 《INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS》 * |
| MOHAMMADS IQBAL,ET AL.: "Preparation, characterization and biological evaluation of copper(Ⅱ) and zinc(Ⅱ) complexes with cephalexin", 《J. PHARM. PHARMACOL.》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104758314A (en) * | 2015-03-10 | 2015-07-08 | 同济大学 | Synergism method of antibacterial performance of ceftazidime by nano silver phosphate |
| CN104758314B (en) * | 2015-03-10 | 2018-04-27 | 同济大学 | A kind of synergisting method of nano silver to cefotaxime bacteriostasis property |
| CN105271359A (en) * | 2015-11-06 | 2016-01-27 | 武汉工程大学 | Nanometer copper oxide and preparation method and application thereof |
| CN110241174A (en) * | 2019-06-19 | 2019-09-17 | 同济大学 | The preparation method of nano magnesia raising kanamycins antibiotic property |
| CN110241174B (en) * | 2019-06-19 | 2022-11-11 | 同济大学 | Preparation method for improving kanamycin antibacterial property by nano magnesium oxide |
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| CN103933066B (en) | 2017-05-24 |
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