CN103926421B - Autocorrelation speed detection device for cell screening - Google Patents
Autocorrelation speed detection device for cell screening Download PDFInfo
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- CN103926421B CN103926421B CN201410187981.5A CN201410187981A CN103926421B CN 103926421 B CN103926421 B CN 103926421B CN 201410187981 A CN201410187981 A CN 201410187981A CN 103926421 B CN103926421 B CN 103926421B
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- grating
- fluidic chip
- spheroidal
- microlens array
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- 238000001514 detection method Methods 0.000 title claims abstract description 18
- 238000012216 screening Methods 0.000 title claims abstract description 17
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Abstract
The invention discloses an autocorrelation speed detection device for cell screening. The autocorrelation speed detection device is characterized by comprising a micro-fluidic chip, a cylindrical micro-lens array, a spheroidal micro-lens array, a micro-channel, a photosensitive device, slits, a grating, a grating fixing groove and a laser beam, wherein the cylindrical micro-lens array, the spheroidal micro-lens array, the micro-channel, the photosensitive device, the slits, the grating, the grating fixing groove and the laser beam are designed and installed inside the micro-fluidic chip. The micro-fluidic chip is a micro laboratory made of PDMS. The micro-channel is machined inside the micro-fluidic chip according to the design requirement. The spheroidal micro-lens array is inlaid on the lower layer of the plane of the micro-channel. The grating is arranged under the spheroidal micro-lens array. The two slits with the design interval and the width are formed in the middle of the grating. A fixing groove for the grating is fixed inside the micro-fluidic chip. The fixing groove for the grating and the micro-fluidic chip are manufactured into a whole and cannot be disassembled. The photosensitive device is arranged below the grating, laid on the lower layer of the micro-fluidic chip, and solidified inside the micro-fluidic chip at the high temperature.
Description
Technical field
The present invention relates to biomedical technology, specially a kind of autocorrelation velocity detection device for cell screening.
Background technology
Cell is the elementary cell of vital movement, is the basis of life science and biomedical research.Study of disease
Root problem with cancer is exactly to study cell, because being that the irregular change of various cells leads to the generation of disease and cancer.
But the size of cell is typically in micron order, it is difficult to observe and operates, all the time, the screening of cell and separation annoying always
The research and development of cell, and detect cell speed just more difficult.Currently, measurement cell movement speed mainly has fluorescence mark
Note observational method and image method.Although these methods are capable of observation of cell, measuring speed, these methods also exist a lot
Not enough.Fluorescent labeling observational method is a kind of most common method, and the fluorescence that this method adds produces to the living environment of cell
Impact, disturbs the orthobiosiss of cell, may result in the change of cell death or cytoactive.Although image method is not
The activity of cell can be affected, but image method real-time less desirable it is impossible to quickly reflect the flow velocity of cell, this is to thin
Screening is a very big technical bottleneck to born of the same parents at a high speed.
Content of the invention
For the deficiencies in the prior art, technical problem that the present invention intends to solve be to provide a kind of for cell screening from
Relevant speed detection device.This device can be good at measuring the speed of screened cell in micro-fluidic chip passage, has inspection
Degree of testing the speed is fast, and intelligence degree is high, the features such as separate complete.
The technical scheme that the present invention solves described technical problem is that a kind of autocorrelation velocity for cell screening of design is visited
Survey device it is characterised in that this device includes the micro-fluidic chip and design and installation cylindrical lenticular battle array in micro-fluidic chip
Row, spheroidal microlens array, microchannel, sensor devices, slit, grating, grating fixing groove and laser beam, micro-fluidic chip
It is the small laboratory being made up of PDMS, need the microchannel being machined with by design in the inside of micro-fluidic chip, in microchannel
Inlay spheroidal microlens array for following one layer of plane, the underface of spheroidal microlens array be grating, in grating
Between have 2 slits of design pitch and width, grating fixing groove is fixed on inside micro-fluidic chip, and with micro-fluidic chip system
Integral, non-dismountable；It is sensor devices in the lower section of grating, sensor devices are laid in the lower floor of micro-fluidic chip, and pass through
Hot setting is inside micro-fluidic chip.
Compared with prior art, present invention proposition autocorrelation velocity detection device overcomes existing detection device well
Deficiency, enables autocorrelation velocity detection device the flowing velocity of cell is quickly and accurately detected, and this screens to high-flux cell
It is a good technical support, and autocorrelation velocity detection device is to be made on micro-fluidic chip, and chip is integrally formed,
Meet small form factor requirements, be easy to carry and actually used.
Brief description
Fig. 1 is a kind of Making programme schematic diagram of embodiment of autocorrelation velocity detection device that the present invention is used for cell screening
(upward view of bottom of device).
Fig. 2 is a kind of grating fixed slot configurations of embodiment of autocorrelation velocity detection device that the present invention is used for cell screening
Schematic diagram（The upward view of bottom of device）.
Fig. 3 is a kind of optical grating construction of embodiment of autocorrelation velocity detection device and the fixation that the present invention is used for cell screening
Slot structure schematic diagram（The upward view of bottom of device）.
When Fig. 4 is the laser guide cell of a kind of embodiment of autocorrelation velocity detection device that the present invention is used for cell screening
System Working Principle schematic diagram（Front view under device normal operating conditions）.
Specific embodiments
Further describe the present invention with reference to embodiment and its accompanying drawing.
The autocorrelation velocity detection device for cell screening of present invention design（Abbreviation device, referring to Fig. 1-4）, it is special
Levy and be that this device includes the micro-fluidic chip 3 and design and installation cylindrical lenticular array 1 in micro-fluidic chip 3, ball
Shape microlens array 2, microchannel 4, sensor devices 5, slit 6, grating 7, grating fixing groove 8 and laser beam 9, micro-fluidic chip
3 is the small laboratory being made up of PDMS, needs the microchannel 4 being machined with by design in the inside of micro-fluidic chip 3, micro- logical
Inlay spheroidal microlens array 2 for following one layer of road 4 plane, be grating 7 in the underface of spheroidal microlens array 2,
2 slits 6 of design pitch S and width are had, grating 7 is fixed on inside micro-fluidic chip 3 with fixing groove 8 in the middle of grating 7, and with
Micro-fluidic chip 3 is formed integrally, non-dismountable；It is sensor devices 5 in the lower section of grating 7, sensor devices 5 are laid in micro-fluidic core
The lower floor of piece 3, and through hot setting inside micro-fluidic chip 3.
Described microchannel 4 is etched in micro-fluidic chip 3 by design requirement, microchannel 4 with diameter greater than cell dia；?
Column type microlens array 1 is inlayed by the lower floor of microchannel 4, and the diameter of column type microlens array 1 is 50 microns, and oneself height is
35 microns, the horizontal interval between column type microlens array 1 is 50 microns；Described cylindrical lenticular array 1 is by poly- first
Base methacrylate（PMMA）Make, after 165 DEG C, the slight baking of 10 minutes, bonding is embedded on micro-fluidic chip 3,
The other end becomes spheroidal microlens array 2, and its Main Function is that the reflected light 11 being emitted onto produce on cell 10 is regular
Be irradiated on grating 7, slit 6 width on grating 7 is 10 microns, and slit 6 is apart from the distance 25 of spheroidal microlens array 2
Micron, the distance between two slits 6 are S, sensor devices 5 are presented herein below in slit 6, sensor devices 5 be coated in micro-fluidic
Inside chip 3, coating thickness is 2.5 microns.
The preparation method of apparatus of the present invention embodiment is mainly：Process microchannel 4 in micro-fluidic chip 3, in microchannel 4
Bottom inlay a diameter of 50 microns, high 35 microns, level interval is 50 microns of cylindrical lenticular array 1, through 165
DEG C, baking in 10 minutes, the lenticule of these cylindrical lenticular arrays 1 gradually forms a diameter of 50 microns, high 30 microns of circle
Grade spherical micro-lens array 2, spheroidal microlens array 2 is presented herein below the grating 7 with slit 6, and the distance between two gaps 6 are
S, grating 7 lower floor is the sensor devices 5 of coating.
The autocorrelation velocity that apparatus of the present invention are used for the microchannel 4 inner cell screening of micro-fluidic chip 3 detects, and has fast
Speed, the simple, feature of automatic measurement cell speed.
The operation principle of apparatus of the present invention and process are：Cell 10 is in the microchannel 4 of micro-fluidic chip 3 due to laser 9
Radiation and move, in moving process, the light being irradiated on cell 10 can light shine spheroidal due to reflection
On microlens array 2, thus generating scattering light 11, movement velocity under laser 9 effect for the different cells 10 is different, thus raw
The scattering light 11 becoming is also different, also different hence into the light in 2 slits 6, and then the light of sensor devices 5 impression is not yet
With the light entering slit 6 is converted into the signal of telecommunication by A/D capture card after sensor devices 5, and the signal of telecommunication is input to external part
On computer, form 2 similar waveforms, the time t through slit 6 for the light can be calculated according to waveform；Again by described two slits 6
Apart from S and light through the time t of slit 6, the speed of service of cell can be calculated.
The present invention does not address part and is applied to prior art.
Claims (3)
1. a kind of autocorrelation velocity detection device for cell screening is it is characterised in that this device includes micro-fluidic chip and sets
Count the spheroidal microlens array being arranged in micro-fluidic chip, microchannel, sensor devices, slit, grating and grating fixing groove,
Micro-fluidic chip is the small laboratory being made up of PDMS, the inside of micro-fluidic chip by design need to be machined with micro- logical
Road, inlays spheroidal microlens array for following one layer in microchannel plane, in the underface of spheroidal microlens array is
2 slits of design pitch and width are had, grating grating fixing groove is fixed on inside micro-fluidic chip in the middle of grating, grating,
And be formed integrally with micro-fluidic chip, non-dismountable；It is sensor devices in the lower section of grating, sensor devices are laid in micro-fluidic core
The lower floor of piece, and through hot setting inside micro-fluidic chip.
2. the autocorrelation velocity detection device for cell screening according to claim 1 is it is characterised in that described ball
Shape microlens array is made up of cylindrical lenticular array, and the height of spheroidal microlens array is 30 microns, and a diameter of 50
Micron, the horizontal interval between two spheroidal microlens arrays is 50 microns, in the lower section 10-25 of spheroidal microlens array
It is grating at micron, the width of slit is 10 microns, sensor devices are that coating is solidificated in inside micro-fluidic chip.
3. the autocorrelation velocity detection device for cell screening according to claim 1 is it is characterised in that described photosensitive
The coating thickness of device is 2.5 microns.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410187981.5A CN103926421B (en) | 2014-05-07 | 2014-05-07 | Autocorrelation speed detection device for cell screening |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410187981.5A CN103926421B (en) | 2014-05-07 | 2014-05-07 | Autocorrelation speed detection device for cell screening |
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| Publication Number | Publication Date |
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| CN103926421A CN103926421A (en) | 2014-07-16 |
| CN103926421B true CN103926421B (en) | 2017-02-08 |
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| CN201410187981.5A Expired - Fee Related CN103926421B (en) | 2014-05-07 | 2014-05-07 | Autocorrelation speed detection device for cell screening |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108993620B (en) * | 2018-05-31 | 2021-01-22 | 京东方科技集团股份有限公司 | Microfluidic chip and microfluidic system |
| CN113740942B (en) * | 2021-08-11 | 2022-08-16 | 广州先进技术研究所 | Micro-lens array grating and preparation method and application thereof |
| CN114100708B (en) * | 2021-11-15 | 2023-03-14 | 矽翔微机电系统（上海）有限公司 | Microfluid concentration sensing chip and microfluid characteristic measuring device |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1737536A (en) * | 2004-08-18 | 2006-02-22 | 深圳大学 | Five-dimensional fluorescent microscope imaging technique |
| WO2010092728A1 (en) * | 2009-02-16 | 2010-08-19 | コニカミノルタオプト株式会社 | Speed measuring device |
| CN101903532A (en) * | 2008-03-24 | 2010-12-01 | 株式会社尼康 | Method for analyzing image for cell observation, image processing program, and image processing device |
| CN101915855A (en) * | 2010-07-30 | 2010-12-15 | 天津大学 | Method and device for detecting flying speeds of particles in cytometer by using secondary light-scattering method |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7051599B2 (en) * | 2004-11-01 | 2006-05-30 | Board Of Trustees Operating Michigan State University | Thermal transient anemometer having sensing cell assembly |
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2014
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1737536A (en) * | 2004-08-18 | 2006-02-22 | 深圳大学 | Five-dimensional fluorescent microscope imaging technique |
| CN101903532A (en) * | 2008-03-24 | 2010-12-01 | 株式会社尼康 | Method for analyzing image for cell observation, image processing program, and image processing device |
| WO2010092728A1 (en) * | 2009-02-16 | 2010-08-19 | コニカミノルタオプト株式会社 | Speed measuring device |
| CN101915855A (en) * | 2010-07-30 | 2010-12-15 | 天津大学 | Method and device for detecting flying speeds of particles in cytometer by using secondary light-scattering method |
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| CN103926421A (en) | 2014-07-16 |
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Granted publication date: 20170208 |