CN103923903A - Preparation method for immobilized microbe oil-spill repairing agent - Google Patents
Preparation method for immobilized microbe oil-spill repairing agent Download PDFInfo
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- CN103923903A CN103923903A CN201310016703.9A CN201310016703A CN103923903A CN 103923903 A CN103923903 A CN 103923903A CN 201310016703 A CN201310016703 A CN 201310016703A CN 103923903 A CN103923903 A CN 103923903A
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- arachidis hypogaeae
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Abstract
A preparation method for an immobilized microbe oil-spill repairing agent is characterized by comprising the following steps: preparing peanut shell-based active carbon; mixing the peanut shell-based active carbon with a seed bacteria liquid to performing adsorption until adsorption is saturated, so as to form a bacteria suspension; adding a sterilized sodium alginate solution with a temperature of 30-40 DEG C into the above bacteria suspension, uniformly mixing, injecting into a CaCl2 solution with a certain concentration by employing an injector, dispersing into balls and performing crosslinking for 12-24 h, so as to obtain immobilized microbe microballoons; and finally flushing the immobilized microbe microballoons with normal saline for 2-4 times to obtain a finished product. According to the preparation method, the biology-based active carbon modified peanut shell is added into a sodium alginate-calcium chloride embedded immobilized carrier for the first time, the mechanical strength and the mass transfer property of the agent are improved, the activity and the degradation efficiency of microbes are improved; also the cost is low, the technology is simple and easy to operate, the reaction conditions are mild, microbes of the prepared repairing agent is not easy to leak, and the prepared repairing agent is good in stability and reusability, has relatively high microbe activity and cell capacity, and has petroleum hydrocarbon degradation rate of 75-92%.
Description
Technical field
The invention belongs to the bioremediation technology field that oil and petroleum products pollute, relate to a kind of preparation method of immobilized microorganism oil spilling renovation agent, a specifically preparation method for the immobilized microorganism oil spilling renovation agent taking sodium alginate-calcium chloride-Pericarppium arachidis hypogaeae matrix activated carbon as embedded immobilization material, repairs for the microorganism of oil and petroleum products Marine Pollution.
Background technology
Along with the development of offshore oil development and sea transport industry, the oil spill accident causing due to the Oil spills of blowout, ships that transport, leakage of knocking ship and oil pipeline to sinking etc. takes place frequently.After oil spill accident occurs, though through physical method (as fence recovery etc.), chemical process (spraying oil spill dispersant etc.) can be removed part surface oil spilling, but remaining petroleum hydrocarbon prolonged stay is in ocean environment, can cause huge persistence infringement (PelletierE to Marine ecosystems and HUMAN HEALTH, DelilleD, Delille B.Crude oil bioremediation in sub-Antarctic intertidal sediments:chemistry and toxicity of oiled residues[J] .Mar.Environ.Res., 2004, 57 (4): 311-327.).
The bioremediation technology of at present petroleum pollution mainly taking microorganism reparation as main (Zhang Ying, etc. the Pollution control technology [J] of marine oil overflow for Xia Wenxiang, vast stretch of wooded country great waves. Qingdao College of Architectural Engineering journal, 2004,25 (1): 54-57.).Investigator finds that growth has more than 200 kind of hydro carbons degrading microorganism in seawater and fresh water both at home and abroad, comprise bacterium, fungi and algae, closely related (Fingas M.The Basic of Oil Spill Clean up (2nd edition) [M] the .USA:Lewis Publishers of oil pollution degree in its density and reproduction speed and waters, 2001,39-50.).Microbial treatment technology is the efficient remedying oil-polluted new technology of generally acknowledging in the world, can adapt to the ecotope of various complexity based on microorganism, its breeding metabolic capacity is extremely strong, various toxic substances in energy fast degradation oil, and there is many advantages (the Harayama S such as cheap, environmental friendliness, Kasai Y, Hara A.Microbial communities in oil-contaminated seawater[J] .Curr.Opin.Biotechnol., 2004,15 (3): 205-214.).The oil spilling that adopts this technical finesse physics and chemistry method to remove is the optimal path of Revegetation of Eco-environment, and to protection China marine eco-environment, the sustainable development that maintains oceanic resources has great scientific meaning and using value.
From the seventies in last century, add suitable microbial treatment petroleum pollution be just considered to a strong strategy (Atlas RM.Petroleum biodegradation and oil spill bioremediation[J] .Mar.Pollut.Bull., 1995, 31 (4-12): 178-182.), in the wild in test successfully example as: nineteen ninety utilizes AlphaBioSea to process the crude oil leakage of Texas seashore, within 2000, utilize Terra-zyme (TM) to process Japanese Nakhodka harbour crude oil leakage (Maki H, Hirayama N, Hiwatari T, etal.Crude oil bioremediation field experiment in the sea of Japan[J] .Mar.Pollut.Bull., 2003, 47 (1-6): 74-77.), summer to 1991 in 1989 year application add nutrition agent and efficient alkane degradation bacterium to prince ExxonValdez bay, Alaska because oil tanker is revealed processing that the pollution that causes carries out etc., obtained very significantly effect (Atlad RM.Bioremediation of Petroleum Pollutants[J] .Int.Biodeter.Biodegrad., 1995, 35 (3): 317-327.).
But in marine oil spill microorganism repair process, but existing effective strain runs off serious, remediation efficiency significantly descends degradation problem (De-Bashan L E, Bashan Y.Immobilized microalgae for removing pollutants:Review of practical aspects[J] .Bioresour.Technol., 2010,101 (6): 1611-1627.).So, repair peculiar processing environment for marine oil spill, this patent adopts microbial immobilized technology.Immobilized microorganism technique is compared with other bioremediation technology, can keep microorganism high-density, high reactivity, alleviate or eliminate the loss of microorganism, processing efficiency is high, tolerance to environment is strong, easy control of reaction system, can effectively improve remediation efficiency (the Takeno K that inland sea, target area is permeated with oil pollution microbiobacterial agent, Yamaoka Y, SasakiK.Treatment of oil-containing sewage wastewater using immobilized photosynthetic bacteria[J] .WorldJ.Microbiol.Biotechnol., 2005, 21 (8-9): 1385-1391.).
Embedded immobilization technology is as the one of process for fixation, with absorption method, crosslinking, other immobilization technologies such as covalent coupling method are compared, because preparation method is simple to operation, reaction conditions is gentle easily to be realized, microorganism is difficult for leaking, stability and reusing are good, and there are higher microorganism active and cell-volume (Bao Mutai, Gong Yuanjiao, Li Yiming. the effect [J] of immobilized microorganism in degraded oily(waste)water. Wuhan University Journal (Edition), 2010, 56 (1): 109-144.), immobilization efficiency can reach more than 70%, extensively (Zhang Xiuxia of application at present, Qin Lijiao, Wu Weilin, Deng. the preparation of immobilization oil degradation bacterium and performance study [J] thereof. environmental engineering journal, 2010, 4 (3): 659-664.).Sodium alginate-CaCl
2embedding and polyvinyl alcohol-H
3bO
3embedding is the most frequently used embedding immobilization method (Massalha N, Basheer S, Sabbah I.Effect of adsorption and bead size of immobilized biomass on the rate of biodegradation of phenol at high concentration levels[J] .Ind.Eng.Chem.Res., 2007,46 (21): 6820-6824.).With respect to sodium alginate-CaCl
2entrapping method, polyvinyl alcohol-H
3bO
3though the immobilization preparation physical strength of preparation is higher, work-ing life is longer, but polyvinyl alcohol is because of comparatively difficulty of the strong balling-up of nodulizing, and the saturated boric acid solution for cross-linking polyvinyl alcohol is large to the murder by poisoning of microorganism, can significantly be reduced in microbic activity after immobilization.Sodium alginate-CaCl
2though entrapping method reaction conditions gentleness, embedded material is to no toxic biological, and the cell of embedding has higher activity, and pharmaceutical machine intensity is lower, mass transfer performances is poor.
Pericarppium arachidis hypogaeae accounts for 30% of Quality of Peanuts, and main component is hemicellulose and crude fibre, and both account for 75%～80% of Pericarppium arachidis hypogaeae quality, is that good gac is prepared presoma.Pericarppium arachidis hypogaeae based activated carbon is had to vesicular structure, can improve mass transfer performances, Pericarppium arachidis hypogaeae based activated carbon is joined to sodium alginate-CaCl
2in entrapping method, to improve the physical strength of preparation, at home and abroad have not been reported.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of preparation method of the immobilized microorganism oil spilling renovation agent taking sodium alginate-calcium chloride-Pericarppium arachidis hypogaeae matrix activated carbon as embedded immobilization material, the physical strength and the mass transfer performances that improve preparation by adding Pericarppium arachidis hypogaeae based activated carbon, have the advantages that technique is simple, material cost is low.
The present invention solves the problems of the technologies described above adopted technical scheme: a kind of preparation method of immobilized microorganism oil spilling renovation agent, is characterized in that with sodium alginate-CaCl
2-Pericarppium arachidis hypogaeae based activated carbon carries out embedding as carrier, and concrete steps are:
1) prepare Pericarppium arachidis hypogaeae based activated carbon;
2) by appropriate Pericarppium arachidis hypogaeae based activated carbon with plant daughter bacteria liquid in mass ratio 1:3～1:8 mixes and adsorbs, until adsorb saturated, formation bacteria suspension;
3), toward the sterilized 100mL sodium alginate soln (concentration is 3wt%～4wt%) that adds 30～40 DEG C in above-mentioned 10mL bacteria suspension, after mixing, draw and be injected into the certain density CaCl of 500mL with syringe
2in solution (concentration is 3wt%～4wt%), disperse balling-up, crosslinked 12h～24h, being fixed microbe microsphere;
4) finally by the immobilized microorganism microballoon obtaining normal saline flushing 2～4 times, be finished product.
As improvement, the preparation process of described Pericarppium arachidis hypogaeae based activated carbon is:
A, raw material Pericarppium arachidis hypogaeae cleaning, sieve are chosen, be dried, are pulverized, mistake 25～35 mesh sieves, for subsequent use;
B, take the above-mentioned Pericarppium arachidis hypogaeae crushing, joining massfraction is the zinc chloride activation agent solution (mass ratio of Pericarppium arachidis hypogaeae based activated carbon and liquor zinci chloridi is 1:2～1:5) of 30-50%, stirring and evenly mixing, at room temperature floods approximately 0.5～1.5h by the feed liquid mixing;
C, the feed liquid of having flooded is put into muffle furnace activate;
D, the sample having activated is taken out from muffle furnace, cooling after, sample is poured into and in hydrochloric acid soln (concentrated hydrochloric acid of 11～13mol/L and water volume ratio are 1:8～10), is soaked 15-30min, then by sample with 70～80 DEG C of hot washes to pH neutrality;
E, the sample having washed is put into electric heating convection oven, dry 3～5h at 100～120 DEG C, after taking-up, be placed in moisture eliminator cooling;
F, the sample after the activation making was pulverized to 150～250 mesh sieves, made Pericarppium arachidis hypogaeae based activated carbon.
As preferably, in described step C, activation temperature is 500～700 DEG C, and the temperature rise rate that rises to required activation temperature from room temperature is 40～60 DEG C/min, insulation 30～90min.
Preferred again, described kind of daughter bacteria liquid is using Brevibacillus brevis D-1 as petroleum hydrocarbon degradation bacterium, and the concentration of planting daughter bacteria liquid is 5～8.5 × 10
9cell/g.
Finally, the concentration of described use sodium alginate is 3wt%～4wt%, CaCl
2strength of solution is 3wt%～4wt%, and the mass percent concentration of the shared immobilized microspheres of Pericarppium arachidis hypogaeae based activated carbon is 0.6-1.8wt%, and in described immobilized microorganism oil spilling renovation agent, the microorganism concn of embedding is 6 × 10
7-8 × 10
8cell/g.
Compared with prior art, the invention has the advantages that:
1, first by bio-based activated carbon in---modified peanut is added into sodium alginate---calcium chloride entrapped immobilized carrier, improve the physical strength of preparation, and due to the vesicular structure of Pericarppium arachidis hypogaeae based activated carbon, can greatly improve its mass transfer performances, make nutritive substance more easily by microorganism panning, improved greatly microbic activity and degradation efficiency.
2, modified peanut based activated carbon is compared with general activated carbon, and raw material sources are more extensive, and price is more cheap, and makes the performance of immobilized microorganism more excellent.
3, preparation method is simple to operation, reaction conditions gentleness, and the renovation agent microorganism making is difficult for leaking, and stability and reusing are good, and have higher microorganism active and cell-volume, can reach 75-92% to the degradation rate of petroleum hydrocarbon.
Brief description of the drawings
Fig. 1 be immobilized microorganism oil spilling renovation agent of the present invention fabrication processing figure;
Fig. 2 is stereoscan photograph of the present invention, wherein a is Pericarppium arachidis hypogaeae based activated carbon stereoscan photograph, b is the blank microballoon stereoscan photograph of embedded immobilization, and c is embedded immobilization microbial microballoon stereoscan photograph, and d is the outward appearance photo (diameter 4-5mm) of embedded immobilization microbial microballoon.
Embodiment
Below in conjunction with accompanying drawing, embodiment is described in further detail the present invention.
Examples of implementation 1
A preparation method for immobilized microorganism oil spilling renovation agent taking sodium alginate-calcium chloride-Pericarppium arachidis hypogaeae matrix activated carbon as embedded immobilization material, concrete steps are:
1, the preparation of Pericarppium arachidis hypogaeae matrix activated carbon
1) cleaning of raw material Pericarppium arachidis hypogaeae, sieve are chosen, are dried, are pulverized, cross 30 mesh sieves, for subsequent use.
2) take the above-mentioned Pericarppium arachidis hypogaeae crushing, join massfraction and be 40% zinc chloride activation agent solution (mass ratio of Pericarppium arachidis hypogaeae and liquor zinci chloridi is 1:3), stirring and evenly mixing, at room temperature floods about 1h by the feed liquid mixing;
3) feed liquid of having flooded is put into high temperature muffle furnace, rise to 600 DEG C of required activation temperatures from room temperature, temperature rise rate is 50 DEG C/min, insulation 45min.
4) sample having activated is taken out from high temperature muffle furnace, cooling after, sample is poured into and is soaked 20min in hydrochloric acid soln (concentrated hydrochloric acid of 12mol/L and water volume ratio are 1:9), then by sample with 75 DEG C of hot washes to closely neutrality of pH.
5) sample having washed is put into electric heating convection oven, 110 DEG C are dried 4h, cooling in moisture eliminator.
6) sample after the activation making was pulverized to 200 mesh sieves, and made Pericarppium arachidis hypogaeae based activated carbon, the stereoscan photograph of Pericarppium arachidis hypogaeae based activated carbon is shown in Fig. 2 (a).
2. the petroleum hydrocarbon degradation bacterium adopting
The petroleum hydrocarbon degradation bacterium that embedding is used is the bacterial classification Brevibacillus brevis D-1 filtering out from certain Oceanic Samples, is 25-35 ‰ in salinity, and temperature is 15-25 DEG C, in the environment that pH is 7.5-8.5, can reach 35-55% to the degradation rate of crude oil (concentration 1-5g/L).
3, embedding (Fig. 1 is shown in technical process)
1) by the appropriate Pericarppium arachidis hypogaeae based activated carbon making with plant daughter bacteria liquid according to mass ratio 1:4 mixes adsorb until adsorb saturated, formation bacteria suspension;
2), toward the sterilized 100mL sodium alginate soln (concentration is 3wt%～4wt%) that adds 30～40 DEG C in above-mentioned 10mL bacteria suspension, after mixing, draw and be injected into the certain density CaCl of 500mL with syringe
2in solution (concentration is 3wt%～4wt%), disperse balling-up, crosslinked 12h～24h, being fixed microbe microsphere.
3) finally by the immobilized microorganism microballoon obtaining normal saline flushing 3 times, be finished product.
Examples of implementation 2
A preparation method for immobilized microorganism oil spilling renovation agent taking sodium alginate-calcium chloride-Pericarppium arachidis hypogaeae matrix activated carbon as embedded immobilization material, concrete steps are:
1, the preparation of Pericarppium arachidis hypogaeae matrix activated carbon
1) cleaning of raw material Pericarppium arachidis hypogaeae, sieve are chosen, are dried, are pulverized, cross 30 mesh sieves, for subsequent use.
2) take the above-mentioned Pericarppium arachidis hypogaeae crushing, join massfraction and be 50% zinc chloride activation agent solution (mass ratio of Pericarppium arachidis hypogaeae and liquor zinci chloridi is 1:2), stirring and evenly mixing, at room temperature floods about 0.5h by the feed liquid mixing;
3) feed liquid of having flooded is put into high temperature muffle furnace, rise to 700 DEG C of required activation temperatures from room temperature, temperature rise rate is 50 DEG C/min, insulation 30min.
4) sample having activated is taken out from high temperature muffle furnace, cooling after, sample is poured into and is soaked 15min in hydrochloric acid soln (concentrated hydrochloric acid of 13mol/L and water volume ratio are 1:10), then by sample with 80 DEG C of hot washes to closely neutrality of pH.
5) sample having washed is put into electric heating convection oven, 110 DEG C are dried 4h, cooling in moisture eliminator.
6) sample after the activation making was pulverized to 200 mesh sieves, and made Pericarppium arachidis hypogaeae based activated carbon, the stereoscan photograph of Pericarppium arachidis hypogaeae based activated carbon is shown in Fig. 2 (a).
2. the petroleum hydrocarbon degradation bacterium adopting
The petroleum hydrocarbon degradation bacterium that embedding is used is the bacterial classification Brevibacillus brevis D-1 filtering out from certain Oceanic Samples, is 25-35 ‰ in salinity, and temperature is 15-25 DEG C, in the environment that pH is 7.5-8.5, can reach 35-55% to the degradation rate of crude oil (concentration 1-5g/L).
3, embedding (Fig. 1 is shown in technical process)
1) by the appropriate Pericarppium arachidis hypogaeae based activated carbon making with plant daughter bacteria liquid according to mass ratio 1:8 mixes adsorb until adsorb saturated, formation bacteria suspension;
2), toward the sterilized 100mL sodium alginate soln (concentration is 3wt%～4wt%) that adds 30～40 DEG C in above-mentioned 10mL bacteria suspension, after mixing, draw and be injected into the certain density CaCl of 500mL with syringe
2in solution (concentration is 3wt%～4wt%), disperse balling-up, crosslinked 12h, being fixed microbe microsphere.
3) finally by the immobilized microorganism microballoon obtaining normal saline flushing 4 times, be finished product.
Examples of implementation 3
A preparation method for immobilized microorganism oil spilling renovation agent taking sodium alginate-calcium chloride-Pericarppium arachidis hypogaeae matrix activated carbon as embedded immobilization material, concrete steps are:
1, the preparation of Pericarppium arachidis hypogaeae matrix activated carbon
1) cleaning of raw material Pericarppium arachidis hypogaeae, sieve are chosen, are dried, are pulverized, cross 30 mesh sieves, for subsequent use.
2) take the above-mentioned Pericarppium arachidis hypogaeae crushing, join massfraction and be 30% zinc chloride activation agent solution (mass ratio of Pericarppium arachidis hypogaeae and liquor zinci chloridi is 1:2), stirring and evenly mixing, at room temperature floods about 1.5h by the feed liquid mixing;
3) feed liquid of having flooded is put into high temperature muffle furnace, rise to 500 DEG C of required activation temperatures from room temperature, temperature rise rate is 50 DEG C/min, insulation 60min.
4) sample having activated is taken out from high temperature muffle furnace, cooling after, sample is poured into and is soaked 30min in hydrochloric acid soln (concentrated hydrochloric acid of 11mol/L and water volume ratio are 1:8), then sample is closely neutral to pH with 70-80 DEG C of hot wash.
5) sample having washed is put into electric heating convection oven, 110 DEG C are dried 4h, cooling in moisture eliminator.
6) sample after the activation making was pulverized to 200 mesh sieves, and made Pericarppium arachidis hypogaeae based activated carbon, the stereoscan photograph of Pericarppium arachidis hypogaeae based activated carbon is shown in Fig. 2 (a).
2. the petroleum hydrocarbon degradation bacterium adopting
The petroleum hydrocarbon degradation bacterium that embedding is used is the bacterial classification Brevibacillus brevis D-1 filtering out from certain Oceanic Samples, is 25-35 ‰ in salinity, and temperature is 15-25 DEG C, in the environment that pH is 7.5-8.5, can reach 35-55% to the degradation rate of crude oil (concentration 1-5g/L).
3, embedding (Fig. 1 is shown in technical process)
1) by the appropriate Pericarppium arachidis hypogaeae based activated carbon making with plant daughter bacteria liquid according to mass ratio 1:3 mixes adsorb until adsorb saturated, formation bacteria suspension;
2), toward the sterilized 100mL sodium alginate soln (concentration is 3wt%～4wt%) that adds 30～40 DEG C in above-mentioned 10mL bacteria suspension, after mixing, draw and be injected into the certain density CaCl of 500mL with syringe
2in solution (concentration is 3wt%～4wt%), disperse balling-up, crosslinked 24h, being fixed microbe microsphere.
3) finally by the immobilized microorganism microballoon obtaining normal saline flushing 2 times, be finished product.
In above 3 examples of implementation, embedded immobilization microbial microballoon stereoscan photograph is shown in Fig. 2 (c), and Fig. 2 (d) is shown in by outward appearance photo.The microorganism concn of the immobilized microorganism preparation embedding making is after tested 5 × 10
7-7 × 10
8cell/g, is 5-55 ‰ in salinity, and temperature is 10-30 DEG C, in the environment that pH is 6.5-9.0, can reach 75-92% to the degradation rate of petroleum hydrocarbon, and with free microbial ratio, its degradation rate improves 37%-46%, and the tolerance of environment is strengthened.
The immobilized microorganism microballoon below the present invention being made and other immobilized microorganism microballoon and common inorganic active carbon that does not add modified peanut based activated carbon compare, and comparative result is as shown in the table:
The Performance Ratio of the different immobilized microorganism microballoons of table 1
As can be drawn from Table 1, the immobilized microorganism microballoon of interpolation modified peanut based activated carbon of the present invention improves a lot on physical strength and mass-transfer performance, and the cell of embedding has higher activity, can reach 75-92% to the degradation rate of petroleum hydrocarbon.
Claims (5)
1. a preparation method for immobilized microorganism oil spilling renovation agent, is characterized in that carrying out embedding using sodium alginate-CaCl2-Pericarppium arachidis hypogaeae based activated carbon as carrier, and concrete steps are:
1) prepare Pericarppium arachidis hypogaeae based activated carbon;
2) by appropriate Pericarppium arachidis hypogaeae based activated carbon with plant daughter bacteria liquid according to mass ratio 1:3～1:8 mixes adsorb until adsorb saturated, formation bacteria suspension;
3) be 3 (wt) %～4 (wt) % sodium alginate solns toward adding sterilized 100mL, the concentration of 30～40 DEG C in above-mentioned 10mL bacteria suspension, after mixing, with syringe draw and be injected into 500mL, concentration is 3(wt) CaCl of %～4 (wt) %
2in solution, disperse balling-up, crosslinked 12h～24h, being fixed microbe microsphere.
4) finally by the immobilized microorganism microballoon obtaining normal saline flushing 2～4 times, be finished product.
2. preparation method according to claim 1, is characterized in that the preparation process of described Pericarppium arachidis hypogaeae based activated carbon is:
A, raw material Pericarppium arachidis hypogaeae cleaning, sieve are chosen, be dried, are pulverized, mistake 25～35 mesh sieves, for subsequent use;
B, take the above-mentioned Pericarppium arachidis hypogaeae crushing, joining massfraction is the zinc chloride activation agent solution of 30-50%, and the mass ratio of Pericarppium arachidis hypogaeae and liquor zinci chloridi is 1:2～1:5, and the feed liquid mixing is at room temperature flooded approximately 0.5～1.5h by stirring and evenly mixing;
C, the feed liquid of having flooded is put into muffle furnace activate;
D, the sample having activated is taken out from muffle furnace, cooling after, sample is poured in the concentrated hydrochloric acid of 11～13mol/L and aqueous hydrochloric acid that water volume ratio is 1:8～10 and is soaked 15-30min, after taking-up again by sample with 70～80 DEG C of hot washes to pH neutrality;
E, the sample having washed is put into electric heating convection oven, dry 3～5h at 100～120 DEG C, after taking-up, be placed in moisture eliminator cooling;
F, the sample after the activation making was pulverized to 150～250 mesh sieves, made Pericarppium arachidis hypogaeae based activated carbon.
3. preparation method according to claim 2, is characterized in that the activation temperature in described step C is 500～700 DEG C, and the temperature rise rate that rises to required activation temperature from room temperature is 40～60 DEG C/min, insulation 30-90min.
4. preparation method according to claim 1, is characterized in that described kind of daughter bacteria liquid is using Brevibacillus brevis D-1 as petroleum hydrocarbon degradation bacterium, and the concentration of planting daughter bacteria liquid is 5～8.5 × 10
9cell/g.
5. preparation method according to claim 1, the concentration that it is characterized in that described use sodium alginate is 3(wt) %～4(wt) %, CaCl
2strength of solution is 3 (wt) %～4 (wt) %, and the mass percent concentration of the shared immobilized microspheres of Pericarppium arachidis hypogaeae based activated carbon is 0.6-1.8%, and in described immobilized microorganism oil spilling renovation agent, the microorganism concn of embedding is 6 × 10
7-8 × 10
8cell/g.
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| CN113308458A (en) * | 2021-04-26 | 2021-08-27 | 山东交通学院 | Composite material for adsorbing and degrading sea surface oil stains and preparation method thereof |
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| CN106520750A (en) * | 2016-12-08 | 2017-03-22 | 浙江海洋大学 | Immobilized microbial repairing agent for spilled oil and preparation method thereof |
| CN108018229A (en) * | 2017-11-09 | 2018-05-11 | 中南大学 | A kind of hexavalent chrome reduction immobilized microbial inoculum and preparation method |
| CN113308458A (en) * | 2021-04-26 | 2021-08-27 | 山东交通学院 | Composite material for adsorbing and degrading sea surface oil stains and preparation method thereof |
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