CN103913436A - Micro/nano-particle-filled optical microfluidic-chip biosensor - Google Patents
Micro/nano-particle-filled optical microfluidic-chip biosensor Download PDFInfo
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- CN103913436A CN103913436A CN201310019563.0A CN201310019563A CN103913436A CN 103913436 A CN103913436 A CN 103913436A CN 201310019563 A CN201310019563 A CN 201310019563A CN 103913436 A CN103913436 A CN 103913436A
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Abstract
The invention provides a micro/nano-particle-filled optical microfluidic-chip biosensor. The biosensor is characterized in that an optical chamber is filled with small spheres with micro-nano dimension, so that the chamber inner surface area and the surface adsorption quantity are increased, and further the test sensitivity is improved; the chamber can be filled with small spheres with two or more dimension for adjusting the optical chamber surface area and the sphere distance; and the biosensor is applicable to refractive index test of surface adsorbents.
Description
Technical field
The present invention relates to the refraction index test of gas and fluid sample (such as protein, DNA, antigen, antibody etc.) in micro-fluidic chip, can be applicable to environmental monitoring, chemical analysis and the field such as biomedical.
Background technology
Harmful gas detects has important application aspect environmental protection, and liquid detecting has important application at biomedical sector.Micro-fluidic chip can be used as the desirable passage of carrying gas and fluid sample; And its volume is little, can realize trace detection, promote microminiaturization, the integrated and portability of analytical instrument.
Refractive index is an important parameter of reflection gas and liquid property, and the refractive index of test sample just can be known the relevant information such as type and concentration of sample; And optical sensor has the advantages that antijamming capability is strong and response is fast.Therefore, extensively adopt at present optical sensor to carry out refractometry to the sample in micro-fluidic chip.The people such as Kobori (J.Am.Chem.Soc., 2004,126:557) utilize surface plasma resonance sensor (SPR) to detect double-stranded DNA.The people such as Liang (Appl.Phys.Lett., 2005,86:151122) utilize fiber-optic grating sensor to measure the refractive index of isopropyl alcohol.Fabry-Perot (FP) chamber that the people such as Guo (Appl.Phys.Lett., 2011,98:041104) utilize two fiber end faces to form, tests the refractive index of intracavity liquid.We propose and have realized a kind of micro-fluidic control chip sensor (Appl.Phys.Lett., 2012,100:233705) with integrated FP chamber, by improving the quality factor of resonator cavity, thereby significantly improve the resolution of sensor.
FP cavity sensor is easy to the body refractive index (being the mean refractive index of material in chamber) of material in test chamber, but is bad at test surfaces refractive index (being the refractive index of similar spr sensor test surfaces adsorbent).Therefore design research and development are applicable to the FP cavity sensor of test surfaces refractive index, are that wound of the present invention is ground motivation.
Summary of the invention
The present invention is intended to solve the surface refractive index test problem of above-mentioned FP cavity sensor, " a kind of optics microfluidic chip biosensor of filling based on micro-nano particle " proposed, by fill micro-nano size small spherical particles in optics cavity, increase inner cavity surface long-pending, thereby increase test substance, the raising measurement sensitivity of adsorption.
Sensor construction of the present invention as shown in Figure 1, is filled after bead in optics cavity, and the L × W × H when surface area of optics cavity is never filled brings up to the L × W × H+N × π × D after filling
2(wherein L, W and H are respectively length, width and the height in rectangular optical chamber, and D is the small ball's diameter, and N is bead quantity).Therefore fill bead and can significantly improve the surface area in chamber, thereby improve measurement sensitivity (note: because ball contacts as some contact with plane with ball, ball, its contact area is very little, negligible).
Sensor of the present invention, in the time that test substance is flowed through optics cavity by entrance and exit (Fig. 1), test substance can be adsorbed on the surface of optics cavity inwall and bead, the mean refractive index that causes material in whole optics cavity changes, thereby changes the light wave intensity and the phase place that see through optics cavity.Therefore by transmitted light wave intensity or the phase place in measuring optical chamber, just can know the refractive index of adsorbent in chamber, thereby obtain the information such as composition and concentration of test substance.
Sensor of the present invention can first pass into the organic substance of gaseous state or liquid before test, at the adsorption last layer organic substance of optics cavity inwall and bead, thereby increases the adsorptive power of surface to test substance.
Bead of the present invention, its material preferred index approaches the solid matter (as materials such as glass, quartz and polystyrene) of 1.33 (being the refractive index of water), thereby reduces the Discussion on Light Diffusion Caused causing due to the introducing of bead.
Sensor of the present invention, (D in Fig. 2 and bead d) combine to fill optics cavity inside can to select multiple diameter.Shown in Fig. 2 is the combination of two kinds of diameter beads, in order to regulate and to optimize the spacing of ball and ball, thereby regulates the adsorbance of test substance on ball surface.
Optics cavity of the present invention, its entrance and exit size can be less than the diameter of ball, to avoid bead to be taken to (Fig. 2) outside chamber by fluid; Can there be multiple outlets and entrance, allow fluid be more evenly distributed (Fig. 2).
Bead of the present invention, can be by the method for pressurization, heating or finishing, allows mini-pellets be polymerized to a block, can not scatter, thereby improves stable testing degree and avoid bead to be washed away by liquid.
Optics cavity of the present invention exports and entrance, can be designed to the pyramidal structure (Fig. 3) of gradual change, to obtain liquid flow stably.
In the present invention, the arrangement mode of the inner bead of optics cavity except ordered arrangement, also can be random and unordered, its mechanism that increases surface area is consistent with effect.
Accompanying drawing explanation
Accompanying drawing, it is incorporated into and becomes the part of this instructions, the embodiments of the invention of having demonstrated, and explain principle of the present invention with aforesaid summary together with detailed description below.
Fig. 1 is the sensor construction of only filling a kind of bead.
Fig. 2 is the sensor construction of filling two kinds of diameter beads and multiple gateways.
Fig. 3 is the sensor construction of importing and exporting as taper.
Embodiment
For making technical scheme of the present invention more clear, describe the specific embodiment of the present invention in detail below in conjunction with testing scheme and accompanying drawing.
Example 1
First, prepare structure micro-fluidic control chip sensor as shown in Figure 1 on silicon substrate, wherein the material of bead is glass.
Secondly, in optics cavity, first pass into aqueous solution, and then pass into the aqueous solution of test substance; Test substance can be at the Adsorption on Surface of cavity wall and bead, thereby changes the ensemble average refractive index of material in chamber and the phase place through the light wave in chamber.
Finally, see through the phase place of light wave by test, know refractive index and the composition of test substance.
Example 2
First, prepare structure micro-fluidic control chip sensor as shown in Figure 2 in glass substrate, wherein the material of bead is organic plastics.
Secondly, first pass into the solution such as glucosan in optics cavity, dextran molecule can be adsorbed on the surface of cavity wall and bead, to improve the adsorptive power on cavity wall and bead surface; And then passing into the standard solution that contains test substance, test substance can be at the Adsorption on Surface of cavity wall and bead, thereby changes the ensemble average refractive index of material in chamber and the light wave intensity through chamber.
Finally, see through light wave intensity by test, know refractive index and the concentration of test substance.
Example 3
First, prepare structure micro-fluidic control chip sensor as shown in Figure 3 on organic plastics substrate, wherein the material of bead is quartz.
Secondly, in optics cavity, first pass into air, and then pass into the air that contains alcoholic ingredient; Alcohol can be at the Adsorption on Surface of cavity wall and bead, thereby changes the ensemble average refractive index of material in chamber and the phase place through the light wave in chamber.
Finally, see through the phase place of light wave by test, know the concentration of alcohol.
In sum, optics microfluidic chip biosensor provided by the invention, by fill bead in chamber, effectively increases surface area in chamber and the adsorbance of test substance, thereby significantly improves measurement sensitivity.
The above is know-why and instantiation that the present invention applies, the equivalent transformation doing according to conception of the present invention, as long as when its scheme of using does not exceed spiritual that instructions and accompanying drawing contain yet, and all should be within the scope of the invention, explanation hereby.
Claims (8)
1. an optics microfluidic chip biosensor of filling based on micro-nano particle, is characterized in that: by fill the bead of micro-nano size in optics cavity, amass and surface excess, to improve measurement sensitivity thereby increase inner cavity surface; Can in chamber, fill the bead of two kinds and two or more sizes, regulate the surface area of optics cavity; Be applicable to the refraction index test of adsorption material.
2. sensor claimed in claim 1, in the time that test substance is flowed through optics cavity, test substance can be adsorbed on the surface of optics cavity inwall and bead, causes that the mean refractive index of material in whole optics cavity changes, thereby changes the light wave intensity and the phase place that see through optics cavity.
3. optics cavity claimed in claim 1 can first pass into the organic substance of gaseous state or liquid before test, at the adsorption last layer organic substance of optics cavity inwall and bead, thereby increases the adsorptive power of surface to test substance.
4. optics cavity claimed in claim 1, its entrance and exit size can be less than the diameter of ball, to avoid bead to be taken to outside chamber by fluid.
5. optics cavity claimed in claim 1, can have multiple outlets and entrance, allows fluid be more evenly distributed.
6. optics cavity claimed in claim 1, its outlet and entrance can be designed to taper, and fluid is more evenly distributed.
7. bead claimed in claim 1, can be by the method for pressurization, heating or finishing, allows mini-pellets be polymerized to a block, thereby improves stable testing degree and avoid bead to be washed away by fluid.
8. sensor claimed in claim 1, the arrangement mode of bead can be ordered arrangement, also can be random and unordered arrangement in optics cavity.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310019563.0A CN103913436A (en) | 2013-01-09 | 2013-01-09 | Micro/nano-particle-filled optical microfluidic-chip biosensor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310019563.0A CN103913436A (en) | 2013-01-09 | 2013-01-09 | Micro/nano-particle-filled optical microfluidic-chip biosensor |
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| CN103913436A true CN103913436A (en) | 2014-07-09 |
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| CN201310019563.0A Pending CN103913436A (en) | 2013-01-09 | 2013-01-09 | Micro/nano-particle-filled optical microfluidic-chip biosensor |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108084366A (en) * | 2017-12-18 | 2018-05-29 | 哈尔滨工业大学 | Colorimetric fluorescent microsphere emulsion preparation method based on octaethylporphyrin platinum and the application in optical oxygen sensing micro-fluidic detection chip |
| CN115656150A (en) * | 2022-09-26 | 2023-01-31 | 苏州浦隆生物有限公司 | Device and method for quantitatively detecting biomolecules by microfluidic hypersensitive chemiluminescence |
-
2013
- 2013-01-09 CN CN201310019563.0A patent/CN103913436A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108084366A (en) * | 2017-12-18 | 2018-05-29 | 哈尔滨工业大学 | Colorimetric fluorescent microsphere emulsion preparation method based on octaethylporphyrin platinum and the application in optical oxygen sensing micro-fluidic detection chip |
| CN115656150A (en) * | 2022-09-26 | 2023-01-31 | 苏州浦隆生物有限公司 | Device and method for quantitatively detecting biomolecules by microfluidic hypersensitive chemiluminescence |
| CN115656150B (en) * | 2022-09-26 | 2023-09-08 | 苏州浦隆生物有限公司 | Device and method for detecting biomolecules |
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Application publication date: 20140709 |