CN103908533A - Anti-oxidation traditional Chinese medicine composition, preparation method and application - Google Patents
Anti-oxidation traditional Chinese medicine composition, preparation method and application Download PDFInfo
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Abstract
A disclosed anti-oxidation traditional Chinese medicine composition comprises the following bulk drugs by weight: 20-60 parts of natto, 5-30 parts of grape seeds and 5-30 parts of ginkgo biloba leaves. The anti-oxidation traditional Chinese medicine composition is prepared from natural traditional Chinese medicinal materials which are safe, less in side effects and uneasy to cause drug resistance. The anti-oxidation traditional Chinese medicine composition fulfils functions of resisting oxidation and reducing blood fat by reducing level of triglyceride (TG), total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in peripheral blood, raising high-density lipoprotein cholesterol (HDL-C) level, removing free radicals, inhibiting lipid oxidation and employing other means.
Description
Technical field
The present invention relates to Chinese medicine composition of a kind of antioxidation, blood fat reducing and preparation method thereof, belong to the field of Chinese medicines.
Background technology
The aged industry summit forum data demonstration of first China, Chinese aging degree is accelerated day by day, and aging population are aging trend simultaneously.Cai Meiqin etc. are by the nutritional survey to District of Shanghai old people, result demonstration, middle-aged and elderly people antioxidants intake is generally on the low side, and (SOD) is on the low side for serum superoxide dismutases, (LPO) is higher for Serum LPO Levels, illustrates that body anti-oxidation function is poor.Therefore, if the long-term appropriate exogenous antioxidant of supplementing, can compensate endogenous antioxidant level (as SOD) on the one hand, increasing along with the inside and outside source property oxidation preventive content of body on the other hand, can remove in time too much oxygen-derived free radicals in body, suppress lipid peroxidation, to promoting person in middle and old age's human body health to be highly profitable.
Because the factors such as the variation of dietary habit cause crowd's Hyperlipidemia, cardiovascular disease also very common.Chinese medicine hyperlipidemia is all generally with dialectical demonstrations of mode compatibility such as invigorating spleen to remove dampness, reducing phlegm and fever, YIN nourishing and the production of body fluid promoting, is in fact mainly the index such as TC, LDL-C, TG reducing in blood fat, and the indexs such as rising HDL-C, ApoA are object.
Therefore, for current suitable crowd's present situation, develop there is antioxidation, the Chinese medicine composition of auxiliary lipid-lowering function be very applicable to the market demand.
Summary of the invention
The object of this invention is to provide a kind of Anti-oxidation teaditional Chinese medicine compositions and preparation technology and purposes.
This Chinese medicine composition is taking natto, Folium Ginkgo and Semen Vitis viniferae as primary raw material, by above raw extract and pharmaceutically acceptable adjuvant be evenly mixed.
The Chinese medicine composition of antioxidation of the present invention, blood fat reducing, comprises the crude drug of following weight proportion:
Natto 20-60 part, Semen Vitis viniferae 5-30 part, Folium Ginkgo 5-30 part.
Preferably, composition weight proportioning of the present invention is:
Natto 30-50 part, Semen Vitis viniferae 10-25 part, Folium Ginkgo 10-25 part.
More preferred, composition weight proportioning of the present invention is:
35 parts of nattos, 15 parts of Semen Vitis viniferae, 15 parts of Folium Ginkgos.
Or described composition weight proportioning is:
40 parts of nattos, 20 parts of Semen Vitis viniferae, 20 parts of Folium Ginkgos.
Compositions of the present invention can add the adjuvant of following weight proportion:
Filler 10-30 part, absorbent 1-10 part, binding agent 1-15 part, lubricant 2-8 part.
Preferably, described filler is one or more in pregelatinized Starch, starch, lactose; Described absorbent is the one or two in calcium carbonate, calcium hydrogen phosphate; Described binding agent is one or more in polyvinylpyrrolidone, starch slurry, HPMC; Described lubricant can be one or more in magnesium stearate, Pulvis Talci, differential silica gel.
Crude drug of the present invention can obtain from open market, or adopts with the following method and make:
1. natto powder: by selected defilming soybean, water cleans 2-5 time, soaks 18-24 hour, grinds a little in rearmounted cooker and boils, and carries out sterilizing (120～130 DEG C of sterilising temps, sterilization time 50min), natural cooling after sterilizing with high-pressure sterilizing pot.Bacillus natto is inoculated in the Semen sojae atricolor after sterilizing by a certain percentage, and ferment 16～24 hours (temperature 35-45 DEG C, humidity 70-85%), obtains ripe natto.Ripe bean is placed in cold room and is cooled to 5-10 DEG C, take out, put in freeze dryer and dry, pulverize, cross 40-100 mesh sieve, to obtain final product.
2. Semen Vitis viniferae extract: Semen Vitis viniferae is pulverized, with micro-twice (temperature is not less than 85 DEG C) of extraction of boiling of 30-70% ethanol, each 2 hours, filtered while hot, macroporous resin separates, and ethanol gradient elution is collected eluent, concentrated (50～80 DEG C of temperature, vacuum 0.04-0.08MPa), recovered alcohol to pol is 40-45 degree, spraying is dry, cross 60-100 order and sieve, to obtain final product.
3. Folium Ginkgo extract: Folium Ginkgo is pulverized, doubly measured 50-80% alcohol reflux 2-4 time with 10-15, extract 2-5 hour at every turn, filter, merging filtrate, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 2-5 and doubly measures, and upper amide post separates, and 50-70% ethanol elution to terminal, is collected ethanol elution, and decompression recycling ethanol (55-65 DEG C, 0.08Mpa) is to concentrated solution density 1.-1.25.Concentrated solution drying under reduced pressure (60-70 DEG C, 0.08Mpa), pulverizes, and crosses 60-120 mesh sieve, and get final product.
Formula of the present invention composition is taking Chinese Traditional Medicine health preserving theory and modern pharmacology achievement as foundation.In natto, contain the many kinds of substances such as nattokinase, protease, multivitamin, gamma glutamyl transpeptidase, γ-polyglutamic acid; also contain higher active substance, as isoflavone, superoxide dismutase (SOD), Saponin element, tocopherol, dipicolinic acid, V simultaneously
k2deng.Natto element suppresses the formation of arterial thrombus, improves fibrinolytic activity, has thrombus dissolving, blood fat reducing, reduction cholesterol, antitumor, antioxidation, sterilizes, prevents and treats the plurality of health care functions such as osteoporosis, blood pressure lowering, raising liver function and beauty treatment.In Semen Vitis viniferae, mainly contain polyatomic phenol and flavone, aminoacid, protein, the compositions such as vitamin and trace element, have higher nutritive value and medical value, have blood fat reducing, antioxidation, removing free radical, the effect such as anticancer.Main component procyanidin in Semen Vitis viniferae extract is famous, safe and effective with high-efficiency low-toxicity, high bioavailability.It not only can remove free radical, but also the effect of helpful preservation and regeneration VC, VE; The procyanidin holding time in vivo reaches 72h, and only 3h of the holding time of VC, this will strengthen the ability of its removing free radical greatly.Procyanidin in vivo its antioxidation, to remove the ability of free radical be 50 times, ascorbic 20 times of vitamin E.Folium Ginkgo extract has the blood circulation of improvement, the Reperfusion injury that resists myocardial ischemia injures the functions such as the normal Radical Metabolism of protection hepatic tissue.
The present invention selects safety, few side effects, and the natural Chinese medicine that is difficult for drug resistance processes.It is by reducing peripheral blood triglyceride (TG), T-CHOL (TC), low-density lipoprotein cholesterol (LDL-C) level, high density lipoprotein increasing cholesterol (HDL-C) level, remove free radical, suppress the aspect such as lipid oxidation reach antioxidation, blood fat reducing function.
Chinese medicine composition provided by the invention, has done Toxicological evaluation, functional evaluation experiment.
One, blood fat reducing animal experiment
1. materials and methods
1.1 laboratory animals: SPF level healthy SD rat, female.Provided laboratory animal production licence by Laboratory Animal Science portion of Department Of Medicine, Peking University: SCXK(capital) 2006-0008, laboratory animal occupancy permit: SYXK(capital) 2007-0008.Feeding environment is barrier level, 23 DEG C～24 DEG C of experimental situation temperature, humidity 54 %～58 %.
1.2 high lipid foods: 78.8% normal feedstuff, 1% cholesterol, 10% yolk powder and 10% Adeps Sus domestica, 0.2% cholate.
1.3 test methods: experimental group give compositions prepared by the embodiment of the present invention 1 respectively with the sample of 533,267,133 mg/kgBW dosage to the continuous gavage of rat of high lipid food feed 30 days, high fat matched group gives high lipid food and feeds 30 days continuously; Basis matched group gives isopyknic Fructus Maydis oil, feeds continuously 30 days.
1. 4 detect index: rat body weight, TC, TG and HDL-C
1. 5 data statisticss: adopt SPSS software to carry out data statistic analysis to testing result.
2. result
The impact of 2.1 samples on rat body weight
The body weight change of each group rat before and after table 1 experiment (
)
Note: * and the comparison of high fat matched group, P<0.05.
As seen from Table 1, before experiment, the body weight of each group rat is more balanced, no significant difference between group.Experiment to body weight and the gain in weight of the 21st, 30 days high fat control rats is greater than basic matched group, and difference has significance (P<0.05); Experiment was to the 21st, 30 days, the body weight of the each dosage group of sample rat is less than high fat matched group, but the body weight of each dosage group and gain in weight and high fat matched group be there was no significant difference (P<0.05) relatively, shows that the body weight gain of the rat of this sample to high lipid food feed has no significant effect.
The impact of 2.2 samples on serum total cholesterol (TC) content
Before the each group of table 2 rat experiment and the 30th day TC content (
)
| Dosage group (mg/kgBW) | Number of animals (only) | TC(mmol/L before experiment) | P value | The 30th day TC (mmol/L) | P value |
| 533 | 10 | 2.00±0.32 | 1.000 | 2.12±0.65 | 0.037 |
| 267 | 10 | 2.01±0.30 | 0.994 | 2.17±0.33 | 0.063 |
| 133 | 10 | 1.99±0.24 | 1.000 | 2.35±0.45 | 0.295 |
| Basis contrast | 10 | 1.98±0.23 | 1.000 | 2.07±0.48 | 0.02 |
| High fat contrast | 10 | 1.98±0.22 | — | 2.72±0.52 | — |
As seen from Table 2, before experiment, the serum TC content of each group rat is basically identical, the no significant difference (P>0.05) between each group.To the 30th day, high fat matched group TC content was higher than basic matched group in experiment, and difference has significance (P<0.05), showed that hyperlipidemia model sets up.Experiment was to the 30th day, the TC content of the each dosage group of sample rat is all lower than high fat matched group, and the difference of high dose group and high fat matched group has significance (P<0.05), show that this sample has the effect of the serum total cholesterol content of the rat that reduces high lipid food feed.
The impact of 2.3 samples on serum triglycerides (TG) content
Before the each group of table 3 rat experiment and the 30th day TG content (
)
| Dosage group (mg/kgBW) | Number of animals (only) | TG(mmol/L before experiment) | P value | The 30th day TG (mmol/L) | P value |
| 533 | 10 | 1.08±0.50 | 0.994 | 1.49±0.27 | 0.000? |
| 267 | 10 | 1.18±0.51 | 1.000 | 1.54±0.26 | 0.000? |
| 133 | 10 | 1.22±0.56 | 0.994 | 1.97±0.21 | 0.677? |
| Basis contrast | 10 | 1.25±0.59 | 0.980 | 1.37±0.19 | 0.000? |
| High fat contrast | 10 | 1.15±0.49 | — | 2.11±0.49 | — |
[0045]table 3 is visible, and before experiment, each group rat blood serum TG content is basically identical, the no significant difference (P>0.05) between each group.Experiment was to the 30th day, and the TG content of high fat control rats is apparently higher than basic matched group, and difference has very significant (P<0.01), showed hyperlipidemia model establishment.Experiment was to the 30th day, the TG content of the each dosage group of sample rat is all lower than high fat matched group, and the difference of height, middle dosage group and high fat matched group has very significant (P<0.01), show that this sample has the effect of the serum triglycerides content of the rat that reduces high lipid food feed.
The impact of 2.4 samples on serum High Density Lipoprotein Cholesterol (HDL-C) content
Before the each group of table 4 rat experiment and the 30th day HDL-C content (
)
| Dosage group (mg/kgBW) | Number of animals (only) | HDL-C(mmol/L before experiment) | P value | The 30th day HDL-C (mmol/L) | P value |
| 533 | 10 | 0.868±0.083 | 0.302 | 0.981±0.146 | 0.491? |
| 267 | 10 | 0.902±0.091 | 0.759 | 0.924±0.114 | 0.999? |
| 133 | 10 | 0.891±0.070 | 0.612 | 0.913±0.076 | 1.000? |
| Basis contrast | 10 | 0.885±0.063 | 0.517 | 0.890±0.076 | 0.963? |
| High fat contrast | 10 | 0.946±0.183 | — | 0.915±0.124 | — |
As seen from Table 4, before experiment, the Serum HDL-C content of each group rat is basically identical, the no significant difference (P>0.05) between each group.Test to the 30th day the HDL-C content of high fat control rats and basic matched group comparison, no significant difference (P>0.05); The HDL-C content of the each dosage group of sample rat and the comparison of high fat matched group, difference that there are no significant (P>0.05), show this sample on the serum High Density Lipoprotein Cholesterol content of rat without obvious impact.
Conclusion: be equivalent to human body with 533,267,133 mg/kgBW(respectively and recommend 20,10,5 times of consumption) sample of dosage is to the continuous gavage of rat of high lipid food feed 30 days, can reduce serum total cholesterol (TC) and triglyceride (TG) content of rat, body weight gain to rat and high serum High Density Lipoprotein Cholesterol (HDL-C) content have no significant effect, and point out this sample to have the function of auxiliary antilipemic.
Two, antioxidation animal experiment
1. materials and methods
1.1 laboratory animals: SPF level healthy SD old rats, female.Provided laboratory animal production licence by Laboratory Animal Science portion of Department Of Medicine, Peking University: SCXK(capital) 2006-0008, laboratory animal occupancy permit: SYXK(capital) 2007-0008.Feeding environment is barrier level, 23 DEG C～24 DEG C of experimental situation temperature, humidity 54 %～58 %.
1.2 test methods: experimental group give compositions prepared by the embodiment of the present invention 1 respectively with the sample of 533,267,133 mg/kgBW dosage to the continuous gavage of old rats 60 days, matched group gives the sample solution of respective concentration, feeds continuously 60 days; Basis matched group gives isopyknic Fructus Maydis oil, feeds continuously 60 days.
1. 3 detect index: LPO, lipofuscin (Lip), superoxide dismutase (SOD) and glutathion peroxidase (GSH-Px) in rat body weight, serum, brain and liver organization.
1. 4 data statisticss: adopt SPSS software to carry out data statistic analysis to testing result.
2. result
The impact of 2.1 samples on rat body weight
The body weight no significant difference of each group rat before experiment, rat body weight and gain in weight and the negative control group comparison of experiment mid-term and the each dosage group of test ending sample, there are no significant for difference (P>0.05), shows that this sample has no significant effect the body weight of old rats, in table 5.
Table 5 experimental session respectively organize rat body weight change (
)
Note: each body weight and gain in weight comparison of organizing rat in each period in table, there are no significant for difference (P>0.05).
2.2 samples are on rat blood serum and organize the impact of lipid peroxide (LPO) content
Before experiment, the Serum LPO content of each treated animal is more balanced, no significant difference between each group (P>0.05).Experiment is end eventually, lipid peroxidation (LPO) content of serum, brain and the hepatic tissue of the each dosage group of sample rat is all lower than negative control group, wherein the LPO content of high dose group of the height of serum and cerebral tissue, middle dosage group and hepatic tissue and the difference of negative control group have significance (P<0.05), in table 6, show that this sample has the effect of LPO in the serum tissue that reduces old rats.
The serum of the each group of table 6 rat and cerebral tissue lipid peroxide (LPO is in MDA) content (
)
The impact of 2.3 samples on rat tissue's lipofuscin (Lip) content
Experiment is end eventually, the hepatic tissue lipofuscin of the each dosage group of sample rat and cerebral tissue lipofuscin content are all a little less than negative control group, but there are no significant (P>0.05) for the difference of the liver of each dosage group rat, cerebral tissue lipofuscin content and negative control group, in table 7, show this sample to the lipofuscin in old rats tissue (Lip) content without obvious reducing effect.
Lipofuscin in table 7 rat tissue (Lip) content (
)
The impact of 2.4 samples on superoxide dismutase (SOD) vigor in rat blood serum and tissue
Experiment is end eventually, in the serum of the each dosage group of sample rat and liver, cerebral tissue, SOD vigor is all higher than negative Sui Zhao group, and the difference of brain, the SOD of hepatic tissue and the activity of SOD in serum of high dose group and the negative control group of height, middle dosage group has significance, (P<0.05 or P<0.01), in table 8, show that this sample can improve the activity of the superoxide dismutase (SOD) in tissue and the serum of old rats.
The serum of the each group of table 8 rat and liver, cerebral tissue SOD activity (
)
The impact of 2.5 samples on rat tissue's Glutathione Peroxidase (GSH-Px) vigor
Experiment is end eventually, in the serum of the each dosage group of sample rat and liver, cerebral tissue, GSH-Px vigor is a little more than negative control group, but there are no significant for its difference (P>0.05), in table 9, show that glutathion peroxidase (GSH-Px) vigor of this sample to the serum of old rats and in organizing is without significantly rising effect.
The serum of the each group of table 9 rat and liver, cerebral tissue GSH-Px activity (
)
Conclusion: respectively with the sample of 533,267,133 mg/kgBW dosage (be equivalent to human body and recommend 20,10,5 times of consumption) dosage to the continuous gavage of rat of high lipid food feed 60 days, can reduce rat serum and tissue in lipid peroxide (LPO), the activity of the superoxide dismutase (SOD) in serum and the tissue of raising rat, the vigor of the glutathion peroxidase (GSH-Px) in the body weight to rat and tissue in lipofuscin (Lip) content, serum and tissue has no significant effect, and points out this sample to have antioxidative function.
Detailed description of the invention
Embodiment 1
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 120 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 35 DEG C of temperature, humidity 85%, ferments 16 hours, is cooled to 5 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 40% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 50 DEG C, concentrated under 0.08MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 15 times of amount 80% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 2 times of amounts, and upper amide post separates, and 50% ethanol elution to terminal, is collected ethanol elution, and 55 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 20 parts of above-mentioned natto powders, 30 parts of Semen Vitis viniferae extracts, 30 parts of Folium Ginkgo extract, add 10 parts of filleies, 5 parts of absorbent, 15 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 2
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 20 hours, steaming and decocting after grinding, 130 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, temperature 45 C, humidity 70%, ferments 16 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 70% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 80 DEG C, concentrated under 0.08MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 50-70% ethanol elution to terminal, is collected 65 DEG C of ethanol elution, and 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 25 parts of above-mentioned natto powders, 25 parts of Semen Vitis viniferae extracts, 30 parts of Folium Ginkgo extract, add 15 parts of filleies, 5 parts of absorbent, 5 parts of binding agents, 6 parts of lubricants, to obtain final product.
Embodiment 3
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 130 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 35 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 40% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.05MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 15 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 4 times of amounts, and upper amide post separates, and 70% ethanol elution to terminal, is collected ethanol elution, and 65 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 30 parts of above-mentioned natto powders, 20 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 15 parts of filleies, 5 parts of absorbent, 10 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 4
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 130 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 80%, ferments 20 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 70 DEG C, concentrated under 0.04MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 15 times of amount 80% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 70% ethanol elution to terminal, is collected ethanol elution, and 65 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 35 parts of above-mentioned natto powders, 25 parts of Semen Vitis viniferae extracts, 25 parts of Folium Ginkgo extract, add 10 parts of filleies, 10 parts of absorbent, 10 parts of binding agents, 5 parts of lubricants, to obtain final product.
Embodiment 5
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 35 parts of above-mentioned natto powders, 20 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 30 parts of filleies, 10 parts of absorbent, 5 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 6
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 20 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 40 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 35 parts of above-mentioned natto powders, 15 parts of Semen Vitis viniferae extracts, 15 parts of Folium Ginkgo extract, add 30 parts of filleies, 10 parts of absorbent, 5 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 7
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 120 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 80%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 40 parts of above-mentioned natto powders, 20 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 30 parts of filleies, 8 parts of absorbent, 5 parts of binding agents, 6 parts of lubricants, to obtain final product.
Embodiment 8
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 45 parts of above-mentioned natto powders, 20 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 20 parts of filleies, 5 parts of absorbent, 5 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 9
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 40 parts of above-mentioned natto powders, 15 parts of Semen Vitis viniferae extracts, 15 parts of Folium Ginkgo extract, add 25 parts of filleies, 5 parts of absorbent, 5 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 10
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 80 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 40 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 45 parts of above-mentioned natto powders, 25 parts of Semen Vitis viniferae extracts, 25 parts of Folium Ginkgo extract, add 10 parts of filleies, 10 parts of absorbent, 10 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 11
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 120 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 80%, ferments 24 hours, is cooled to 5 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 45 parts of above-mentioned natto powders, 15 parts of Semen Vitis viniferae extracts, 15 parts of Folium Ginkgo extract, add 15 parts of filleies, 5 parts of absorbent, 5 parts of binding agents, 5 parts of lubricants, to obtain final product.
Embodiment 12
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 50 parts of above-mentioned natto powders, 20 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 30 parts of filleies, 10 parts of absorbent, 10 parts of binding agents, 58 parts of lubricants, to obtain final product.
Embodiment 13
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, temperature 45 C, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 70% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 55 parts of above-mentioned natto powders, 10 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 20 parts of filleies, 5 parts of absorbent, 5 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 14
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 55 parts of above-mentioned natto powders, 15 parts of Semen Vitis viniferae extracts, 20 parts of Folium Ginkgo extract, add 30 parts of filleies, 10 parts of absorbent, 10 parts of binding agents, 8 parts of lubricants, to obtain final product.
Embodiment 15
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf:
By defilming soybean, soak 18 hours, steaming and decocting after grinding, 125 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing, 40 DEG C of temperature, humidity 85%, ferments 24 hours, is cooled to 10 DEG C, dry, pulverize, and obtains natto powder;
Semen Vitis viniferae is pulverized, more than 85 DEG C, 50% ethanol extraction twice, macroporous resin separates, and ethanol gradient elution is collected eluent, and 60 DEG C, concentrated under 0.06MPa, reclaiming ethanol to pol is 45 degree, dry, obtains Semen Vitis viniferae extract.
Folium Ginkgo is pulverized, with 10 times of amount 60% alcohol reflux, filtered, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 5 times of amounts, and upper amide post separates, and 60% ethanol elution to terminal, is collected ethanol elution, and 60 DEG C, 0.08Mpa decompression recycling ethanol to concentrated solution density is 1.25, dry, pulverize, and obtains Folium Ginkgo extract.
(2) take 60 parts of above-mentioned natto powders, 20 parts of Semen Vitis viniferae extracts, 15 parts of Folium Ginkgo extract, add 15 parts of filleies, 10 parts of absorbent, 5 parts of binding agents, 8 parts of lubricants, to obtain final product.
Claims (10)
1. a Chinese medicine composition for antioxidation, blood fat reducing, comprises the crude drug of following weight proportion: natto 20-60 part, Semen Vitis viniferae 5-30 part, Folium Ginkgo 5-30 part.
2. Chinese medicine composition according to claim 1, is characterized in that, described compositions comprises the crude drug of following weight proportion: natto 30-50 part, Semen Vitis viniferae 10-25 part, Folium Ginkgo 10-25 part.
3. Chinese medicine composition according to claim 1 and 2, is characterized in that, described compositions comprises the crude drug of following weight proportion: 35 parts of nattos, 15 parts of Semen Vitis viniferae, 15 parts of Folium Ginkgos; Or 40 parts of nattos, 20 parts of Semen Vitis viniferae, 20 parts of Folium Ginkgos.
4. the preparation method of the Chinese medicine composition described in the arbitrary claim of claim 1-3, comprises the steps:
(1) extract natto, Semen Vitis viniferae and effective component of ginkgo leaf;
(2) take in proportion above-mentioned effective ingredient, add pharmaceutically acceptable adjuvant, to obtain final product;
The described natto extracting method of step (1) is: by defilming soybean, soak 18-24 hour, steaming and decocting after grinding, 120～130 DEG C of sterilizing 50min, natural cooling after sterilizing, is inoculated in Bacillus natto in the Semen sojae atricolor after sterilizing temperature 35-45 DEG C, humidity 70-85%, ferment 16～24 hours, be cooled to 5-10 DEG C, dry, pulverize, to obtain final product.
5. preparation method according to claim 4, is characterized in that, the described Semen Vitis viniferae extracting method of step (1) is: Semen Vitis viniferae is pulverized, more than 85 DEG C, 30-70% ethanol extraction twice, macroporous resin separates, ethanol gradient elution, collect eluent, 50～80 DEG C, concentrated under 0.04-0.08MPa, reclaiming ethanol to pol is 40-45 degree, dry, to obtain final product.
6. according to the preparation method described in claim 4 or 5, it is characterized in that, the described Folium Ginkgo extracting method of step (1) is: Folium Ginkgo is pulverized, doubly measured 50-80% alcohol reflux with 10-15, filter, decompression filtrate recycling ethanol is extremely without alcohol taste.Concentrated solution is diluted with water to 2-5 and doubly measures, and upper amide post separates, and 50-70% ethanol elution to terminal, is collected ethanol elution, 55-65 DEG C, and 0.08Mpa decompression recycling ethanol to concentrated solution density is 1-1.25, dry, pulverize, and to obtain final product.
7. Chinese medicine composition according to claim 4, is characterized in that, described Chinese medicine composition comprises the adjuvant as weight proportion: filler 10-30 part, absorbent 1-10 part, binding agent 1-15 part, lubricant 2-8 part.
8. Chinese medicine composition according to claim 7, is characterized in that, described filler is one or more in pregelatinized Starch, starch, lactose; Or
Preferably, described absorbent is one or both in calcium carbonate, calcium hydrogen phosphate; Or
Preferably, described binding agent is one or more in polyvinylpyrrolidone, starch slurry, HPMC; Or
Preferably, described lubricant is one or more in magnesium stearate, Pulvis Talci, micropowder silica gel.
9. the application of the Chinese medicine composition described in the claims in the present invention 1-8 aspect antioxidation.
10. the application of the Chinese medicine composition described in the claims in the present invention 1-8 aspect blood fat reducing.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104256583A (en) * | 2014-09-05 | 2015-01-07 | 洛阳华以生物工程有限公司 | Formula of health-care product adopting natto freeze-dried powder and ginkgo biloba extract as main materials |
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| CN1695652A (en) * | 2004-05-12 | 2005-11-16 | 陕西爱波卓科技有限责任公司 | High performance oxidation resistant compsn. extracted from plant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1695652A (en) * | 2004-05-12 | 2005-11-16 | 陕西爱波卓科技有限责任公司 | High performance oxidation resistant compsn. extracted from plant |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104256583A (en) * | 2014-09-05 | 2015-01-07 | 洛阳华以生物工程有限公司 | Formula of health-care product adopting natto freeze-dried powder and ginkgo biloba extract as main materials |
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