CN103908507A - Traditional Chinese medicine perfusion fluid as well as preparation method and application thereof - Google Patents
Traditional Chinese medicine perfusion fluid as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine perfusion fluid which comprises the following components in parts by weight: 80 parts of herba epimedii, 60 parts of actinolite, 60 parts of salviae miltiorrhizae, 60 parts of flos carthami, 60 parts of leonurus, 60 parts of angelica sinensis, 60 parts of astragalus membranaceus, 60 parts of semen cuscutae and 60 parts of peach kernels. The invention further provides a preparation method and application of the perfusion fluid. The perfusion fluid disclosed by the invention has the beneficial effects that (1) according to the actual production requirements of cattle rearing industry, a novel formula is formed to effectively solve the key link in actual production of cattle rearing industry: the ovary disease infertility of cows suitable for reproduction, so that the conception rate in estrus of cows suitable for reproduction is improved, and the conception rate reaches up to 82.04%; (2) according to the Chinese veterinarian acupoint theory, the medicine gives the maximum effect by different delivery manners: uterus perfusion or acupoint injection; (3) a novel preparation which not only can be perfused in uterus but also can be injected from acupoints is refined by virtue of a water extract and alcohol precipitate method by means of a modern pharmaceutical preparation technology, so that the existing production process is improved, and the purpose of quality control can be realized.
Description
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Technical field
The present invention relates to poultry preparation, be specifically related to a kind of Chinese medicinal perfusion liquid and its preparation method and application.
Background technology
Along with the raising of China's living standards of the people, livestock breeding industry has become one of fastest-rising industry of China's agriculture field, and cattle aquaculture is one of fastest-rising industry in animal husbandry, is playing the part of more and more key player in national economy.Especially the cultivation scale of beef cattle and milch cow is increasing, and cultivation level progressively improves.According to China-styled Certain Industry Field research network industry research information, end 2010, China's beef cattle breeding stock has just exceeded 12353.2 ten thousand, and beef total output occupies the 4th, the world; 2008, national milch cow livestock on hand reached 1233.5 ten thousand, and milk yield reaches 3,870 ten thousand tons, and milk output occupies third place in the world, becomes milk big producing country.In cattle aquaculture, increase a cattle quantity and just seem particularly important, making to breed the cow pregnancy of on time oestrusing becomes important step in cattle aquaculture.
Ovarian disease barrenness is to cause that to breed During Estrous Cycle abnormal, and reproductive performance is low, causes the one of the main reasons that cow is infertile.Wherein mainly comprise the various diseases such as cow ovary is static, retained corpus luteum, ovarian cyst, alternately growth of follicle, delayed ovulation.Because this type of disease incidence is high, affect cattle-raising more serious, so all pay close attention to very much always both at home and abroad.N.Hollis regularly adds up 3172 cow head incidences, find within 150 days puerperal only ovarian cyst cattle just have 210, sickness rate is 6.26%; The reports such as Ahmadljaz, milch cow ovarian cyst incidence rate is about 6-19%.Northeast Agricultural University is investigated 6158 cow heads, suffers from 325 of ovarian disease cattle, and sickness rate is 5.29%, wherein incomplete 124 of Monitoring Ovarian Function, account for 38.15% of ovarian disease, 45 of retained corpus luteums, account for 13.85%, 123 of ovarian cyst, account for 37.85%, follicle is alternately grown 25, accounts for 7.69%, 8 of delayed ovulations, account for 2.46%.Lanzhou Livestock and Animal Drug Inst., Chinese Academy of Agricultural Science is to Heilungkiang, Tianjin, Jiangsu, Hunan, Gansu, Sichuan, Shaanxi, the provinces such as Hebei, 21596 adult cows of 83 cattle farms in city are investigated, 1830 of the not calvers causing because of ovarian disease, account for 8.45%, wherein 555 of retained corpus luteums, account for 30.32% of ovariopathy, static 443 of ovary, account for 24.20%, 379 of ovarian cyst, account for 21.69%, 184 of ovarian atrophies, account for 10.05%, 64 of delayed ovulations, account for 3.50%, ovary, 70 of salpingitis, account for 3.82%, ovary and fallopian tube Other diseases (ovarian tumor, fallopian tube scleroma, adhesion etc.) 135, account for 7.38%.As can be seen here, ovarian disease barrenness is cattle commonly encountered diseases and frequently-occurring disease, records by current document, and the economic loss that China causes because of primary disease every year reaches several hundred million units.
Below enumerate the prior art relevant for the present invention, and evaluate and analyse:
1, prior art one related to the present invention:
the technical scheme of prior art one:
What treat clinically the infertile card of cattle ovary at present is mainly application hormonotherapy.As Prostaglandin F2a (PGF2a) and analog thereof are acknowledged as the choice drug for the treatment of retained corpus luteum, there is good luteolytic effect.With 20-30mg PGF2a or the intramuscular injection of 0.5-1.0mg Cloprostenol, most cows are luteolysis in 3-5 days after injection.Gonadotropin releasing hormone (GnRH) and analog thereof are that treatment ovarian cyst and ovary are static, the comparatively desirable medicine of secretly oestrusing.No matter that cystoid, all can apply separately GnRH or with other hormone fit applications.
Lutropin (LH) and human chorionic gonadotropin (hCG) are the comparatively desirable medicines for the treatment of ovarian cyst.It is static that follicle stimulating hormone (FSH) and pregnant mare serum gonadotrop(h)in (PMSG) (PMSG) can be treated ovary.Also have estrogen, intramuscular injection 20-30mg diethylstilbestrol or 5-10mg estradiol are generally effective to secretly oestrus (injection when cattle approaches ovulation again).In addition, also have oxytocin, progesterone (P
4), thyroxine, hydrocortisone etc.
the shortcoming of prior art one:
Apply clinically at present hormone medicine treatment ovarian disease barrenness and have following side effect:
(1) majority carrys out cow ovary active situation according to examination per rectum clinically, due to technical operation person's clinical experience difference, easily causes subjective erroneous judgement, selects medicine hormone improper.So very easily cause that body reproductive hormone regulates disorderly, make cow be difficult for becoming pregnant.
(2) vaccary and cultivation self-employed entrepreneurs, in order to increase economic efficiency, in a large number, repeatedly apply hormone therapy, very easily cow body are damaged.
(3) in a large number, repeatedly apply hormone therapy, easily cause hormone residues in meat, milk, threaten human health.
2, prior art two related to the present invention:
the technical scheme of prior art two:
Clinically except majority application hormonotherapy, certain applications Chinese medicine preparation is treated at present.
1) loose, the progestogenic infusion liquid of promoting the sexual maturity:
Loose and the progestogenic infusion liquid of promoting the sexual maturity is two kinds of recording of " Chinese veterinary pharmacopoeia " (version in 2000).The classical Chinese medicine compound of faling apart for ancient books of promoting the sexual maturity, prescription is Herba Epimedii 6g, Actinolitum (wine is quenched) 6g, Radix Angelicae Sinensis 4g, Rhizoma Cyperi 5g, Herba Leonuri 6g, Semen Cuscutae 5g totally 6 taste medicines.Pulverize, sieve, mix, to obtain final product.Determined curative effect.Progestogenic infusion liquid prescription is Herba Epimedii 400g, Herba Leonuri 400g, Flos Carthami 200g totally 3 taste medicines.Water extract-alcohol precipitation, injects and uses glucose powder 50g, fine straining, and embedding, sterilizing, to obtain final product.From early 1990s, Song great Lu etc. have carried out a series of research to the mechanism of action of progestogenic infusion liquid, and quality control is studied.The use polyamide film chromatography such as Zhang Baokang have been differentiated the Herba Epimedii Flos Carthami in preparation, have differentiated Cortex Phellodendri and the Herba Leonuri in preparation by thin layer chromatography, with spectrophotometry the content of total alkaloids and total flavones in medicament.Flat magnitude is analyzed Icariin content in progestogenic infusion liquid, has set up the content assaying method of icariin in said preparation.
2) novel progestogenic infusion liquid:
The researchs such as Kong Xiangfeng show, novel progestogenic infusion liquid can increase uterine contraction wave amplitude and frequency.Zhou Shuzhen etc. are by measuring the content of icariin, berberine, total alkaloids and dry extract, compared with water extraction, semi-biomimetic method, water extract-alcohol precipitation and the impact of half bionical alcohol deposition method on novel progestogenic infusion liquid extraction effect, result semi-bionic extraction effect is best.
3) the gravidity assisting liquid of promoting the sexual maturity:
A kind of compound Chinese medicinal preparation being formed by the Chinese medicine such as Herba Epimedii, Flos Carthami, Wang Dongcai application said preparation treatment milch cow retained corpus luteum 18 examples, ovarian cyst 12 examples and static 4 examples of ovary, total effective rate reaches 100%, and cure rate reaches 94%.Close beautiful strong grade the quality control of said preparation is studied, set up the content assaying method high performance liquid chromatography of icariin in preparation.Ovary barrenness 61 examples such as the static and ovarian cyst of its treatment milch cow retained corpus luteum for Li Shihong etc., ovary, on average the rate of oestrusing is 99.4%, and pregnancy rate is 81.5%, wherein treats retained corpus luteum 36 examples, and on average the rate of oestrusing is 98.2%, and pregnancy rate is 79.8%; Static 12 examples for the treatment of ovary, on average the rate of oestrusing is 100%, pregnancy rate is 85.4%; Ovarian cyst 13 examples, on average the rate of oestrusing is 100%, pregnancy rate is 79.2%.
the shortcoming of prior art two:
(1) treat at present the effective Chinese traditional patent formulation of cattle ovarian disease barrenness single.Two kinds of recording of " Chinese veterinary pharmacopoeia " (version in 2000) only have 2, for loose and the progestogenic infusion liquid of promoting the sexual maturity, are the classical Chinese medicine compound of ancient books.There is at present scholar to carry out pharmaceutical research.Although determined curative effect, production technology is more extensive, and quality standard need to continue to improve, to carry out large-scale production.
(2) the novel progestogenic infusion liquid in other related documents data, the gravidity assisting liquid of promoting the sexual maturity have report, there is at present part pharmaceutical research, therapeutic effect still can, but treatment number of cases is less, curative effect needs further checking, production technology and quality standard need standardization, and distance reaches " Chinese veterinary pharmacopoeia ", and to include standard also far.
(3) existing treatment cattle ovarian disease barrenness Chinese medicine wholistic therapy poor effect, in other words, is exactly that cattle ovarian disease barrenness cure rate is low, and conception rate is low.
Summary of the invention
Object of the present invention is exactly for above-mentioned defect of the prior art, according to the principle of traditional Chinese veterinary medicine theory and determination for the treatment of based on pathogenesis obtained through differentiation of symptoms and signs, a kind of Chinese medicinal perfusion liquid that can effectively improve suitable numerous cow non-return rate is provided, and is a kind of pure Chinese medicinal preparation for the development of cattle (beef cattle, milch cow) ovarian disease barrenness.
To achieve these goals, technical scheme provided by the invention is: a kind of Chinese medicinal perfusion liquid, described Chinese medicinal perfusion liquid includes following component by weight: 80 parts of Herba Epimedii, 60 parts of Actinolitum, 60 parts of Radix Salviae Miltiorrhizaes, 60 parts, Flos Carthami, 60 parts of Herba Leonuris, 60 parts of Radix Angelicae Sinensis, 60 parts of the Radixs Astragali, 60 parts of Semen Cuscutae, 60 parts, Semen Persicae.
the principles of formulating prescriptions:according to clinical syndrome differentiation typing, ovarian disease barrenness belongs to the stagnation of QI-blood card of insufficiency of kidney-YANG and disharmony of Chong and Conception Channels mostly.Therefore selected to have strengthening yang and invigorating kidney, the Actinolitum of irritability function, Herba Epimedii, Semen Cuscutae, the Radix Angelicae Sinensis of tonifying blood and regulating menstruation, blood circulation promoting and blood stasis dispelling, Herba Leonuri, Semen Persicae, Radix Salviae Miltiorrhizae, Flos Carthami; Also select the Radix Astragali of QI invigorating, invigorating YANG with strengthening the body resistance, thereby reached YIN and YANG in a relative equilibrium, made to suffer from the normal reproductive function of Niu Huifu.
Second object of the present invention has been to provide the preparation method of above-mentioned Chinese medicinal perfusion liquid, be to adopt decoction and alcohol sedimentation technique to prepare Chinese medicinal perfusion liquid each tcm components, and water extraction 2 times, precipitate with ethanol 3 times, makes the clear and bright infusion liquid of 1 gram of every milliliter contained.
Further, the preparation method of above-mentioned Chinese medicinal perfusion liquid, comprises the following steps:
1) get each Chinese medicinal components according to said ratio, after cleaning, add water logging and cross after medical material, flood 12 hours;
2) after dipping step 1) being obtained, medical material decocts, the process of decocting is that intense fire decocts with slow fire after boiled, each 1 hour, decoct 2 times, the 1st time decocts amount of water is quality of medicinal material 7 times, the 2nd amount of water is quality of medicinal material 5 times, collecting decoction, and concentrated obtaining water extraction medicinal liquid, concentrated ratio is: the concentrated water extraction medicinal liquid 560ml that to obtain when Chinese medicinal components gross weight is 560g;
3) by step 2) the water extraction medicinal liquid that obtains, wait 95% ethanol that adds 3 times of volumes after temperature, after stirring evenly, to precipitate, the sedimentation time is 24 hours, discards sediment after getting supernatant distillation, recovered alcohol repeated precipitation 3 times, obtain precipitate with ethanol medicinal liquid;
4) in precipitate with ethanol medicinal liquid step 3) being obtained, add distilled water to make its every 1ml containing crude drug 1g, subpackage autoclaving obtain clear and bright Chinese medicinal perfusion liquid.
The 3rd object of the present invention has been to provide the application of above-mentioned Chinese medicinal perfusion liquid in treatment cattle ovarian disease barrenness.
Further, above-mentioned application, carries out uterine perfusion by above-mentioned Chinese medicinal perfusion liquid, concrete grammar is: by apparatus and trouble cattle external genital routine disinfection, with rectum, hold is sent seme-deposition device into uterus, inject Chinese medicinal perfusion liquid 100ml at every turn, the next day 1 time, be for 3 times 1 course for the treatment of.
Further, above-mentioned application, carries out administration by above-mentioned Chinese medicinal perfusion liquid in the mode of acupoint injection therapy.
Be mainly used in breeding the ovarian disease barrenness such as cow ovary is static, retained corpus luteum, ovarian cyst, alternately growth of follicle, delayed ovulation.
For verifying the effect of Chinese medicinal perfusion liquid provided by the invention, grouping choosing side, treats ovarian dysfunction case 514 examples altogether, and wherein Chinese medicinal perfusion liquid group uterine perfusion of the present invention is treated 323 examples, 294 examples of oestrusing, and 265 examples of being impregnated, conception rate is 82.04%.Substantially approach normal healthy controls cattle.
Beneficial effect of the present invention is: the invention provides a kind of Chinese medicinal perfusion liquid, and its preparation method and application are provided, have the following advantages:
(1) according to cattle-raising needs of production, form new formula, efficiently solve key link in cattle-raising actual production: suitable numerous cow ovarian disease barrenness problem, improve suitable numerous cow non-return rate, conception rate is up to 82.04%;
(2) according to Chinese veterinarian acupuncture point theory, pass through different modes of administration: uterine perfusion or acupoint injection therapy, make medicine bring into play maximum drug effect;
(3) utilize modern pharmaceutical technique, by decoction and alcohol sedimentation technique, being both refined into can uterine perfusion, and new formulation that also can acupoint injection therapy, improves existing production technology, can reach the object of quality controllable system.
According to not clinical experiment observation and the test of pesticide effectiveness of calver of 514 examples, tentatively to think, it is simple that said preparation has production technology, good effect, instant effect, easy to use, the advantage such as have no side effect.Carry out 323 examples of uterine perfusion treatment with Chinese medicinal perfusion liquid provided by the invention, 294 examples of oestrusing (91.02%), 265 examples of being impregnated (82.04%).This kind of therapy is compared with current main therapy, and its conception rate is all higher than other therapies.Large scale application in China.
Said preparation is promoted the use of and can be made suitable numerous cow conceived ahead of time, reduces the nonpregnant time of cow, thereby can save forage grass, feedstuff, improves feedstuff-meat ratio; Save cost of labor, voluminous calf, milch cow fecund milk.
Every 1 cow head reduces by 1 feelings phase, is generally 21 days, and saving forage grass, expecting is 21 days × 30 yuan/day=630 yuan; Save 100 yuan of artificial minimum estimations (each poultry raiser feed 20 cow head meters); Fecund milk is 21 days × 20kg/ days × 4 yuan/kg milk=1680 yuan; Every of calf of fecund is by 1000 yuan.
If by 1233.5 ten thousand calculating of national milch cow in 2008, suppose that suitable numerous cow is 1/2,616.75 ten thousand, ovarian disease barrenness sickness rate is that 5%(is lower than our Investigate incidence), so, just there are 30.8375 ten thousand for ovarian disease barrenness.Be calculated as follows:
Save forage grass and feed to be about=30.8375 ten thousand × 630 yuan=19427.625 ten thousand yuan;
Save artificial=30.8375 ten thousand × 100 yuan=3083.75 ten thousand yuan;
Fecund calf=3.08375 ten thousand × 1000 yuan=30837.5 ten thousand yuan;
Milch cow adds up to 30837.5 ten thousand yuan of 3083.75 ten thousand yuan of ﹢ of=19427.625 ten thousand yuan of ﹢
=53348.875 ten thousand yuan.
Only one of cultured dairy, can produce more than 500,000,000 yuan of economic benefit every year, cultivates annual livestock on hand more than 100,000,000 if add beef cattle, and economic benefit is more considerable.
In addition, meat, the noresidue of Lac Bovis seu Bubali Chinese medicine, be pollution-free food, to improving people's living standard, to the development of cattle-raising, significant, can produce obvious social benefit.
The specific embodiment
embodiment 1:
1, experimental animal:
From cattle farm, area just outside a city gate, Lanzhou, the cattle farm such as the national cattle farm in cattle farm, Linze, seven Li He cattle farms, railway cattle farm, Lanzhou, blue condensed milk cattle farm, Lanzhou, cattle farm, Xining, Tianjin, select milch cow 514 examples of above non-estrus in February in puerperal as treatment target, per rectum checks that determined infertile type is: retained corpus luteum 242 examples, static 180 examples of ovary, ovarian cyst 74 examples, ovarian atrophy 18 examples, grouping is treated with distinct methods.
2, clinical examination:
Row shape, size, quality, position and the contractile response of examination per rectum cornua uteri before treatment, shape, size, quality and the corpus luteum etc. of ovary.
3, lab testing:
Suffer from the mensuration of Ox blood plasma progesterone level; Hemorheological detection; The mensuration of micronutrient levels in blood; The impact experiment of Chinese medicine on female white mice sexual organ development; Medicinal liquid safety experiment.
4, medicine and method for making:
1(contrast side of side): Herba Epimedii 100g, Actinolitum 100g, Radix Salviae Miltiorrhizae 100g, Flos Carthami 100g, Herba Leonuri 100g.
Side 2(the present invention): Herba Epimedii 80g, Actinolitum 60g, Radix Salviae Miltiorrhizae 60g, Flos Carthami 60g, Herba Leonuri 60g, Radix Angelicae Sinensis 60g, Radix Astragali 60g, Semen Cuscutae 60g, Semen Persicae 60g.
Side 1 or side 2 are put into respectively to stainless steel cask, water rinses the dust in medical material, add water logging and cross powder, amount of water is 7 times of quality of medicinal material, flood 12 hours, intense fire (big fire) is boiled, slow fire (little fire) decocts, each 1 hour, decoct 2 times, decoct 5 times that front amount of water is quality of medicinal material for the 2nd time, collecting decoction, be concentrated into 500ml(side 1)/560ml(side 2), wait warm 95% ethanol that adds 3 times of volumes, stir evenly, precipitate 24 hours, get supernatant recovery of alcohol distillation, repeated precipitation 3 times, add distilled water to make its every 1ml containing crude drug 1g gained medicinal liquid.Subpackage, autoclaving.
5, Therapeutic Method:
Side 1, side 2 acupoint injection therapy or uterine perfusion respectively.
1. acupoint injection therapy: get wild goose wing (imitative horse wild goose wing), Houhai acupoint.
Wild goose wing cave: be arranged in hip tuberosity to 1/3 intersection vertical line that dorsal line is done, outer, thrust the about 6cm of gluteus medius left and right, every was for 4 times 1 course for the treatment of.
Houhai acupoint: in the depression between anus and root of the tail.Thrust about 6cm to front upper place, the side of injectioning 1 or just 2 medicinal liquid 20ml, the next day 1 time, be for 4 times 1 course for the treatment of.
2. uterine perfusion: with 0.1% bromo geramine sterilization deferent duct and latex tubing (the latex tubing 0.5m of diameter 0.5cm is connected to deferent duct end), external genital washing and sterilizing, patient holds cervix uteri with rectum hold on the other hand, another hand-held deferent duct is sent into intrauterine, assistant injects intrauterine with 100ml syringe absorption side 1 or side 2 medicinal liquid 100ml, the next day 1 time, be for 3 times 1 course for the treatment of.
6, curative effect determinate standard:
1. cure: during treatment or after treatment, retained corpus luteum, ovarian cyst disappear, and it is normal that Monitoring Ovarian Function recovers, and the semen deposition of oestrusing, the person of becoming pregnant within 3 feelings phases.
2. take a turn for the better: after treatment, retained corpus luteum, ovarian cyst disappear, and Monitoring Ovarian Function recovers normally substantially, though the semen deposition of oestrusing, the person of not becoming pregnant.
3. invalid: after treatment, as before, Monitoring Ovarian Function does not recover normally, and the person of not oestrusing for retained corpus luteum, ovarian cyst.
7, clinical test results:
The treatment cattle ovarian disease novel formulation filtering out, through safety testing, is discovery untoward reaction.Grouping choosing side treatment ovarian dysfunction case 514 examples, side's 1 acupoint injection therapy 40 examples, 32 examples of oestrusing, 13 examples of being impregnated, conception rate is 32.50%; Side's 2 acupoint injection therapy 108 examples, 74 examples of oestrusing, 55 examples of being impregnated, conception rate 50.92%; Side's 1 uterine perfusion 43 examples, 35 examples of oestrusing, 22 examples of being impregnated, conception rate is 51.16%; Side's 2 uterine perfusion 323 examples, 294 examples of oestrusing, the rate of oestrusing is 91.02%, 265 examples of being impregnated, conception rate is 82.04.Result of the test shows, the rate of oestrusing of side's 2 uterine perfusions, conception rate compared with other three groups, significant difference (p < 0.05) and the comparison of normal healthy controls group, the rate of oestrusing and conception rate are substantially approaching.The gravidity assisting filling agent therapeutic outcome of promoting the sexual maturity is as shown in table 1.
Table 1
。
In therapeutic test, side's 2 uterine perfusion groups, treat 323 examples, wherein retained corpus luteum 146 examples, static 117 examples of ovary, ovarian cyst 42 examples, ovarian atrophy 18 examples altogether.Side's 2 uterine perfusion therapeutic outcomes are as shown in table 2.
Table 2
。
Side's 2 uterine perfusion groups and current main therapy comparative result are as shown in table 3.
Table 3
。
8, brief summary:
1. suffer from cattle (point 4 treatment groups) by 514 examples and carry out therapeutic test, the side's of showing 2 uterine perfusion group effects are better.This therapy of evidence has good effect, and instant effect is easy to use, and the advantage such as have no side effect.
2. ovarian disease barrenness case is controlled in this test, according to clinical syndrome differentiation typing, mostly belongs to the stagnation of QI-blood card of insufficiency of kidney-YANG and disharmony of Chong and Conception Channels.Therefore selected to have strengthening yang and invigorating kidney, the Actinolitum of irritability function, Herba Epimedii, Semen Cuscutae, the Radix Angelicae Sinensis of tonifying blood and regulating menstruation, blood circulation promoting and blood stasis dispelling, Herba Leonuri, Semen Persicae, Radix Salviae Miltiorrhizae, Flos Carthami; Also select the Radix Astragali of QI invigorating, invigorating YANG with strengthening the body resistance, thereby reached YIN and YANG in a relative equilibrium, made to suffer from the normal reproductive function of Niu Huifu.
3. result of the test shows, side's 2 uterine perfusion groups not only have good effect to retained corpus luteum, ovary inactive infertility, and the not calver that ovarian cyst, ovarian atrophy are caused also has certain curative effect.Its rate of oestrusing reaches 91.02%, 3 non-return rate and reaches 82.04%.There is aborning promotional value.
Finally it should be noted that: the foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, although the present invention is had been described in detail with reference to previous embodiment, for a person skilled in the art, its technical scheme that still can record aforementioned each embodiment is modified, or part technical characterictic is wherein equal to replacement.Within the spirit and principles in the present invention all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (6)
1. a Chinese medicinal perfusion liquid, is characterized in that, described Chinese medicinal perfusion liquid includes following component by weight: 80 parts of Herba Epimedii, 60 parts of Actinolitum, 60 parts of Radix Salviae Miltiorrhizaes, 60 parts, Flos Carthami, 60 parts of Herba Leonuris, 60 parts of Radix Angelicae Sinensis, 60 parts of the Radixs Astragali, 60 parts of Semen Cuscutae, 60 parts, Semen Persicae.
2. a preparation method for Chinese medicinal perfusion liquid as claimed in claim 1, is characterized in that, be to adopt decoction and alcohol sedimentation technique to prepare Chinese medicinal perfusion liquid each tcm components, and water extraction 2 times, precipitate with ethanol 3 times, makes the clear and bright infusion liquid of 1 gram of every milliliter contained.
3. the preparation method of Chinese medicinal perfusion liquid according to claim 2, is characterized in that, comprises the following steps:
1) get each Chinese medicinal components according to proportioning described in claim 1, after cleaning, add water logging and cross after medical material, flood 12 hours;
2) after dipping step 1) being obtained, medical material decocts, the process of decocting is that intense fire decocts with slow fire after boiled, each 1 hour, decoct 2 times, the 1st time decocts amount of water is quality of medicinal material 7 times, the 2nd amount of water is quality of medicinal material 5 times, collecting decoction, and concentrated obtaining water extraction medicinal liquid, concentrated ratio is: the concentrated water extraction medicinal liquid 560ml that to obtain when Chinese medicinal components gross weight is 560g;
3) by step 2) the water extraction medicinal liquid that obtains, wait 95% ethanol that adds 3 times of volumes after temperature, after stirring evenly, to precipitate, the sedimentation time is 24 hours, discards sediment after getting supernatant distillation, recovered alcohol repeated precipitation 3 times, obtain precipitate with ethanol medicinal liquid;
4) in precipitate with ethanol medicinal liquid step 3) being obtained, add distilled water to make its every 1ml containing crude drug 1g, subpackage autoclaving obtain clear and bright Chinese medicinal perfusion liquid.
4. a Chinese medicinal perfusion liquid as claimed in claim 1 is in the application for the treatment of in cattle ovarian disease barrenness.
5. application according to claim 4, it is characterized in that, Chinese medicinal perfusion liquid as claimed in claim 1 is carried out to uterine perfusion, concrete grammar is: by apparatus and trouble cattle external genital routine disinfection, with rectum, hold is sent seme-deposition device into uterus, each Chinese medicinal perfusion liquid 100ml that injects, the next day 1 time, be for 3 times 1 course for the treatment of.
6. application according to claim 4, is characterized in that, Chinese medicinal perfusion liquid as claimed in claim 1 is carried out to administration in the mode of acupoint injection therapy.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104288314A (en) * | 2014-09-30 | 2015-01-21 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine perfusate for treating infertility of dairy cattle and preparation method thereof |
| CN105106431A (en) * | 2015-09-14 | 2015-12-02 | 石河子大学 | Traditional Chinese medicine perfusion agent for treating cow ovarian quiescence and persistent corpus luteum infertility and preparation method thereof |
| CN116492397A (en) * | 2023-06-08 | 2023-07-28 | 北京九州大地生物技术集团股份有限公司 | Preparation for improving cow breeding performance, preparation method thereof and method for improving cow breeding performance |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100877A (en) * | 2011-03-05 | 2011-06-22 | 中国农业科学院兰州畜牧与兽药研究所 | Medicament for treating ovarian infertility |
-
2014
- 2014-04-15 CN CN201410149820.7A patent/CN103908507A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102100877A (en) * | 2011-03-05 | 2011-06-22 | 中国农业科学院兰州畜牧与兽药研究所 | Medicament for treating ovarian infertility |
Non-Patent Citations (1)
| Title |
|---|
| 蔡守溪等: "《郭光纪中兽医研究文集》", 31 August 2008, article "母牛不孕症病机探讨及诊疗" * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104288314A (en) * | 2014-09-30 | 2015-01-21 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine perfusate for treating infertility of dairy cattle and preparation method thereof |
| CN105106431A (en) * | 2015-09-14 | 2015-12-02 | 石河子大学 | Traditional Chinese medicine perfusion agent for treating cow ovarian quiescence and persistent corpus luteum infertility and preparation method thereof |
| CN116492397A (en) * | 2023-06-08 | 2023-07-28 | 北京九州大地生物技术集团股份有限公司 | Preparation for improving cow breeding performance, preparation method thereof and method for improving cow breeding performance |
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Application publication date: 20140709 |