CN103901201B - Cefalexin residual fluorescence immunochromatographydetecting detecting test strip and preparation thereof - Google Patents
Cefalexin residual fluorescence immunochromatographydetecting detecting test strip and preparation thereof Download PDFInfo
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- CN103901201B CN103901201B CN201410123427.0A CN201410123427A CN103901201B CN 103901201 B CN103901201 B CN 103901201B CN 201410123427 A CN201410123427 A CN 201410123427A CN 103901201 B CN103901201 B CN 103901201B
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- cefalexin
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- ZAIPMKNFIOOWCQ-UEKVPHQBSA-N cephalexin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@@H]3N(C2=O)C(=C(CS3)C)C(O)=O)=CC=CC=C1 ZAIPMKNFIOOWCQ-UEKVPHQBSA-N 0.000 title claims abstract description 86
- 229940106164 cephalexin Drugs 0.000 title claims abstract description 85
- 238000012360 testing method Methods 0.000 title claims abstract description 61
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 238000001514 detection method Methods 0.000 claims abstract description 54
- 239000000427 antigen Substances 0.000 claims abstract description 33
- 102000036639 antigens Human genes 0.000 claims abstract description 33
- 108091007433 antigens Proteins 0.000 claims abstract description 33
- 229940092253 ovalbumin Drugs 0.000 claims abstract description 22
- 239000002105 nanoparticle Substances 0.000 claims abstract description 19
- 108010058846 Ovalbumin Proteins 0.000 claims abstract description 17
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 16
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 16
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 12
- 102000018358 immunoglobulin Human genes 0.000 claims abstract description 12
- 239000003365 glass fiber Substances 0.000 claims abstract description 9
- 230000000274 adsorptive effect Effects 0.000 claims abstract description 7
- 230000015572 biosynthetic process Effects 0.000 claims abstract description 7
- 238000003786 synthesis reaction Methods 0.000 claims abstract description 7
- 239000000523 sample Substances 0.000 claims description 39
- 238000000034 method Methods 0.000 claims description 29
- 210000004080 milk Anatomy 0.000 claims description 16
- 239000008267 milk Substances 0.000 claims description 16
- 235000013336 milk Nutrition 0.000 claims description 16
- 239000000243 solution Substances 0.000 claims description 15
- 241000699670 Mus sp. Species 0.000 claims description 13
- 229940098773 bovine serum albumin Drugs 0.000 claims description 13
- 239000012086 standard solution Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 claims description 11
- 108091003079 Bovine Serum Albumin Proteins 0.000 claims description 11
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 claims description 11
- 241001494479 Pecora Species 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 10
- 241001465754 Metazoa Species 0.000 claims description 9
- 241000699666 Mus <mouse, genus> Species 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 9
- 230000008878 coupling Effects 0.000 claims description 9
- 238000010168 coupling process Methods 0.000 claims description 9
- 238000005859 coupling reaction Methods 0.000 claims description 9
- 239000007788 liquid Substances 0.000 claims description 9
- 239000012528 membrane Substances 0.000 claims description 8
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 7
- 210000004408 hybridoma Anatomy 0.000 claims description 7
- 230000003053 immunization Effects 0.000 claims description 7
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 101100298998 Caenorhabditis elegans pbs-3 gene Proteins 0.000 claims description 6
- 230000004913 activation Effects 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000002671 adjuvant Substances 0.000 claims description 5
- 238000004458 analytical method Methods 0.000 claims description 5
- 210000004027 cell Anatomy 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 229910052761 rare earth metal Inorganic materials 0.000 claims description 5
- 150000002910 rare earth metals Chemical class 0.000 claims description 5
- 238000002965 ELISA Methods 0.000 claims description 4
- 150000001718 carbodiimides Chemical class 0.000 claims description 4
- 239000011248 coating agent Substances 0.000 claims description 4
- 238000000576 coating method Methods 0.000 claims description 4
- 238000005238 degreasing Methods 0.000 claims description 4
- 238000002203 pretreatment Methods 0.000 claims description 4
- 206010003445 Ascites Diseases 0.000 claims description 3
- 102000006395 Globulins Human genes 0.000 claims description 3
- 108010044091 Globulins Proteins 0.000 claims description 3
- 229920001503 Glucan Polymers 0.000 claims description 3
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 3
- 206010068676 Pneumoretroperitoneum Diseases 0.000 claims description 3
- 208000005727 Retropneumoperitoneum Diseases 0.000 claims description 3
- 230000007910 cell fusion Effects 0.000 claims description 3
- 238000010367 cloning Methods 0.000 claims description 3
- 239000003085 diluting agent Substances 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 230000002163 immunogen Effects 0.000 claims description 3
- TWNIBLMWSKIRAT-VFUOTHLCSA-N levoglucosan Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@H]2CO[C@@H]1O2 TWNIBLMWSKIRAT-VFUOTHLCSA-N 0.000 claims description 3
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 3
- 238000000746 purification Methods 0.000 claims description 3
- 239000012488 sample solution Substances 0.000 claims description 3
- 238000005070 sampling Methods 0.000 claims description 3
- 210000002966 serum Anatomy 0.000 claims description 3
- 210000004988 splenocyte Anatomy 0.000 claims description 3
- 238000011282 treatment Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims 2
- HOGDNTQCSIKEEV-UHFFFAOYSA-N n'-hydroxybutanediamide Chemical compound NC(=O)CCC(=O)NO HOGDNTQCSIKEEV-UHFFFAOYSA-N 0.000 claims 2
- 239000010452 phosphate Substances 0.000 claims 2
- 239000008363 phosphate buffer Substances 0.000 claims 2
- IPTOYISYIDUYAC-UHFFFAOYSA-N [C-]#N.[Na+].[B+3].[C-]#N.[C-]#N.[C-]#N Chemical compound [C-]#N.[Na+].[B+3].[C-]#N.[C-]#N.[C-]#N IPTOYISYIDUYAC-UHFFFAOYSA-N 0.000 claims 1
- 238000004132 cross linking Methods 0.000 claims 1
- 230000001900 immune effect Effects 0.000 claims 1
- 230000014759 maintenance of location Effects 0.000 claims 1
- 238000010422 painting Methods 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 abstract description 10
- 238000002372 labelling Methods 0.000 abstract description 5
- 241000283707 Capra Species 0.000 abstract 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 8
- 239000003814 drug Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000003556 assay Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
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- 230000035945 sensitivity Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 2
- 241000283690 Bos taurus Species 0.000 description 2
- ULGZDMOVFRHVEP-RWJQBGPGSA-N Erythromycin Chemical compound O([C@@H]1[C@@H](C)C(=O)O[C@@H]([C@@]([C@H](O)[C@@H](C)C(=O)[C@H](C)C[C@@](C)(O)[C@H](O[C@H]2[C@@H]([C@H](C[C@@H](C)O2)N(C)C)O)[C@H]1C)(C)O)CC)[C@H]1C[C@@](C)(OC)[C@@H](O)[C@H](C)O1 ULGZDMOVFRHVEP-RWJQBGPGSA-N 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 229910052796 boron Inorganic materials 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 239000002274 desiccant Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 238000011065 in-situ storage Methods 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- MNWBNISUBARLIT-UHFFFAOYSA-N sodium cyanide Chemical compound [Na+].N#[C-] MNWBNISUBARLIT-UHFFFAOYSA-N 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 230000001629 suppression Effects 0.000 description 2
- 108010022579 ATP dependent 26S protease Proteins 0.000 description 1
- 102000014914 Carrier Proteins Human genes 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 229920002160 Celluloid Polymers 0.000 description 1
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 206010022678 Intestinal infections Diseases 0.000 description 1
- 239000004100 Oxytetracycline Substances 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 150000001299 aldehydes Chemical class 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000003782 beta lactam antibiotic agent Substances 0.000 description 1
- 238000004166 bioassay Methods 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229940097572 chloromycetin Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 229960003276 erythromycin Drugs 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- 230000005283 ground state Effects 0.000 description 1
- -1 has microbial method Chemical compound 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 208000004396 mastitis Diseases 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 229960000625 oxytetracycline Drugs 0.000 description 1
- IWVCMVBTMGNXQD-PXOLEDIWSA-N oxytetracycline Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(O)=C(C(N)=O)C(=O)[C@@]4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-PXOLEDIWSA-N 0.000 description 1
- 235000019366 oxytetracycline Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- SEEPANYCNGTZFQ-UHFFFAOYSA-N sulfadiazine Chemical compound C1=CC(N)=CC=C1S(=O)(=O)NC1=NC=CC=N1 SEEPANYCNGTZFQ-UHFFFAOYSA-N 0.000 description 1
- 229960004306 sulfadiazine Drugs 0.000 description 1
- IWVCMVBTMGNXQD-UHFFFAOYSA-N terramycin dehydrate Natural products C1=CC=C2C(O)(C)C3C(O)C4C(N(C)C)C(O)=C(C(N)=O)C(=O)C4(O)C(O)=C3C(=O)C2=C1O IWVCMVBTMGNXQD-UHFFFAOYSA-N 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 210000003708 urethra Anatomy 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
- 239000002132 β-lactam antibiotic Substances 0.000 description 1
- 229940124586 β-lactam antibiotics Drugs 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
Abstract
The invention discloses fluorescence immune chromatography test strip and the preparation thereof of a kind of cefalexin residual quantity quick, sensitive, easy and simple to handle, this test strips is included on end liner sequentially mutually pastes sample pad, pad, nitrocellulose filter and adsorptive pads overlap joint, nitrocellulose filter is coated with detection line and nature controlling line, and pad is coated with fluorescently-labeled cefalexin monoclonal antibody.The preparation method of this cefalexin residual fluorescence immunochromatographydetecting detecting test strip includes: the synthesis of cefalexin ovalbumin envelope antigen, the preparation of goat anti-mouse immunoglobulin antibody, is then coated on nitrocellulose filter as detection line and nature controlling line；The preparation of fluorescent nano particle labelling cefalexin monoclonal antibody, after be coated on glass fibre as pad；Backboard sequentially mutually pastes sample pad, pad, nitrocellulose filter and adsorptive pads overlap joint；The test strips obtained cuts into 4mm width and preserves in room temperature.
Description
Technical field
The present invention relates to the fluorescence immunoassay quick test technique of a kind of residue of veterinary drug, particularly relate to a kind of fluorescence immunoassay layer for cefalexin residue detection
Analysis test strip and preparation thereof.
Background technology
Cefalexin (Cephalexin) is beta-lactam antibiotic.Veterinary clinic is widely used in the mastitis controlling milch cow, treats animal urethra, stomach
Intestinal and respiratory tract infection etc..But owing to its using method is improper or does not observe the reasons such as off-drug period regulation, its residual in livestock products all can be caused,
Serious harm is brought to human health.For this medicine residual in food, " the animal food herbal medicine high residue that the Ministry of Agriculture of China issues
Limitation " middle regulation, the MRL in milk is 100 μ g/kg, and the MRL in muscle, fat is 200 μ g/kg.
The method being presently used for detecting cefalexin mainly has microbial method, chromatography or chromatograph-mass spectrometer coupling analytic process, immunoassay etc..Micro-
Bioassay method is simple to operate, but its detection cycle length and resultant error are bigger.Although chromatography or chromatograph-mass spectrometer coupling technology are highly sensitive, but
Sample pre-treatments and measure complex operation, costly, is unsuitable for the quick detection of a large amount of sample.
Coons uses fluorescein to be marked and succeed equal to nineteen forty-one first.This carry out Antigen Location with fluorescent material traget antibody
Technology be referred to as fluorescent antibody technique (fluorescent antibody technique).Fluorescence is claimed by the method for fluorescent antibody spike or inspection corresponding antigens
Antibody act；The fluorescent antigen label spike known with oneself or the method checking corresponding antibodies claim fluorescent antigen method.Both approaches general name immunofluorescence skill
Art, more common with fluorescent antibody method the most again.Based on immunofluorescence technique, the analysis method in combination with chromatography principle is referred to as fluorescence immunoassay
Chromatography, has the advantages such as sensitive, quick, special, easy with it, is widely used in on-site supervision and extensive sample examination.
Summary of the invention
It is an object of the invention to provide a kind of easy and simple to handle, low cost, highly sensitive, can on-site supervision and applicable great amount of samples examination, be used for
The fluorescence immune chromatography test strip of cefalexin drug residue in detection milk.
Cefalexin residual fluorescence immunochromatographydetecting detecting test strip of the present invention, pastes treated sample pad on backboard successively, is coated with
The pad of fluorescent-labeled antibody, it is coated with detection line and the nitrocellulose filter of nature controlling line and adsorptive pads makes cefalexin residual fluorescence immune quantitative
Chromatograph test strip.
Preferably, on described pad, the diameter range of the rare-earth fluorescent nano-particle of coating is 50-100nm.
Described rare-earth fluorescent nano-particle, the wave-length coverage that can launch is 600-630nm, and fluorescent nano particle contains rare-earth complex, in ground state
Under be stable, excitation source (340-365nm) effect under can launch the wave-length coverage fluorescence at 600-630nm.
Preferably, on described pad, the fluorescent nano particle traget antibody of coating is rare-earth fluorescent nanoparticle label cefalexin monoclonal antibody,
The detection line envelope antigen that to be cefalexin prepared with ovalbumin (OVA) it is coated with above nitrocellulose filter.
Another object of the present invention is to provide the preparation method of a kind of cefalexin residual fluorescence immunochromatographydetecting detecting test strip.
The preparation method of cefalexin residual fluorescence immunochromatographydetecting detecting test strip of the present invention, comprises the following steps:
A. the concrete preparation process of cefalexin monoclonal antibody is:
(1) animal immune: employing female BAl BIc/C mouse inbred lines is as immune animal, even with the pure albumen of carrier proteins Bovine with cefalexin
Connection thing is immunogen；
(2) cell merges and cloning: take immune balb/c mice splenocyte and Sp2/0 myeloma cell fusion, screens cell strain, obtains
The hybridoma cell strain of stably excreting monoclonal antibody；
(3) preparation of monoclonal antibody and purification: use female BAl BIc/C mouse inbred lines, lumbar injection incomplete Freund's adjuvant, 5 days pneumoretroperitoneums
Injection hybridoma cell strain 106Individual/only, take mouse ascites after 7 days, after being purified, obtain monoclonal antibody subpackage ,-20 DEG C of preservations.
B. the aldehyde radical of fluorescent nano particle:
Take 2mg fluorescent nano particle (20mg/mL, 100 μ L), with the carbonate buffer solution (pH=9.5) of 25mM wash 3 times (12000r/min,
4 minutes) after be suspended in the 100 above-mentioned carbonate buffer solutions of μ L.Adding the glucosan of 120 μ L aldehyde radicals, mixing, the most ultrasonic, lucifuge is anti-
Answer 3-4 hour (room temperature).After washing 3 times (12000r/min, 4 minutes) with above-mentioned carbonate buffer solution, it is suspended in 100 μ L carbonate buffer
In liquid.1500r/min is centrifuged 1 minute, goes the removal of impurity, is placed on 4 DEG C of standby traget antibodies and is used.
C. the preparation of fluorescent nano particle labelling cefalexin monoclonal antibody:
By 0.5mg cefalexin monoclonal antibody 4 DEG C of dialysed overnight of carbonate buffer solution, mix with the fluorescent nano particle of above-mentioned aldehyde radical, 4 DEG C
Reaction is overnight.Then, adding boron Cyanogran., final concentration of 5mM, 4 DEG C are reacted 2-4 hour.Add isopyknic confining liquid (50mM tris-HCl
PH7.8, containing 2%BSA, 4% sucrose), close 12 hours or overnight；Finally with the antibody 3 that 50mM tris-HCl pH7.8 washing labelling is good
All over (12000r/min, 4 minutes, 4 DEG C), then with 100 μ L50mM tris-HCl pH7.8 (containing 0.9%NaCl, 0.2%BSA, 0.05%
Tween-20) suspending, 4 DEG C of lucifuges store for future use.
D. the pad of fluorescent-labeled antibody it is coated with:
Glass fibre membrane is soaked in 0.2mol/L Tris-HCl (pH7.8), in the treatment fluid of 1.0%TritonX-100,3.0%BSA, in 4 DEG C
Under the conditions of soak 2 hours；Then dry 2 hours at 37 DEG C, standby.Glass fibre membrane is cut into the width of 10mm again, then at Bio-Dot
On XYZ3050 three-dimensional specking platform, it is sprayed onto glass fibre membrane with the Bio-Jet Quanti300 noncontact quantitation nozzle that declines, at 37 DEG C, dries 1
Hour, standby.
E. detection line and nature controlling line are coated on nitrocellulose filter:
Described detection line is the envelope antigen that cefalexin is prepared with ovalbumin (OVA)；By cefalexin and ovalbumin (OVA), use
Glutaraldehyde method carries out coupling and obtains envelope antigen.Take cefalexin 10mg to be dissolved in 5mL PBS (0.01mol/L, pH7.4), add 1% penta
Dialdehyde solution stirring 30 minutes, takes OVA30mg and is dissolved in 1mL PBS, joins in the cefalexin solution of activation, and 25 DEG C of stirring reactions 2 are little
Time, 4 DEG C of reactions are overnight.Under the conditions of 4 DEG C, with PBS 3 days, obtaining cefalexin envelope antigen, subpackage is frozen.Goat-anti BALB/C is little
Rat immune globulin antibody (sheep anti-mouse igg) is as antigen, immune sheep with the immunoglobulin of BALB/C mice, extracts in immune sheep serum
Antibody globulin make.Cefalexin-ovalbumin envelope antigen and goat-anti BALB/C mice immunoglobulin are resisted with being coated diluent respectively
Body is adjusted to 0.3-1.0mg/mL, and film liquid measure is 25 μ L/25-30cm, and using them, as detecting, line is parallel with nature controlling line is sprayed on celluloid
Being coated on film, detection line and nature controlling line interval 4-7mm, then dry 2 hours at 37 DEG C, envelope, in case reagent paper backboard pasting board is used.
F. once mutual the pasting treated sample pad, be coated with the pad of fluorescent-labeled antibody, be coated with detection line of overlap joint on backboard
With nitrocellulose filter and the adsorptive pads of nature controlling line, obtain test strips, be cut into 4mm width as requested, seal standby together with desiccant.
The present invention also provides for a kind of method utilizing described cefalexin residual fluorescence immunochromatographydetecting detecting test strip detection cefalexin residual, including
Following steps:
(1) sample pre-treatments: directly measure after centrifugal (12000r/min, the 10min) defat of milk sample；
(2), with ELISA test strip: the upper sampling point in test strips directly drips above-mentioned centrifugal degreasing milk sample, after chromatographing 15-20 minute, 615nm is surveyed
The fluorescence intensity level at place；
(3) Analysis of test results:
By the fluorescence intensity meansigma methods (H) of the detection line of the standard solution of each concentration obtained divided by first standard solution (0 standard)
Detection line fluorescence intensity level (H0) it is multiplied by 100% again, obtain percentage detection line fluorescence intensity level；Computing formula is: detection line percentage fluorescence is strong
Angle value (%)=(H/H0) × 100%；In formula, H is the detection line average fluorescent strength value of standard solution, H0It it is the inspection of 0 μ g/L standard solution
Survey line average fluorescent strength value；With the semilog value of cefalexin standard concentration as X-axis, detection line percentage detection line fluorescence intensity level is Y-axis,
Draw canonical plotting；Calculate the detection line percentage fluorescence intensity level of sample solution by same way, corresponding go out a sample concentration, from mark
The residual quantity of cefalexin in sample is read on directrix curve.
The Cleaning Principle of the present invention is:
When test sample passes through capillarity siphon from bottom to top along test strips, according to chromatographic theory, in chromatography moving process, through ELISA test strip
End, moves to the other end, successively sequentially passes through and detects line and nature controlling line arrival adsorptive pads on pad, nitrocellulose filter.After chromatography, if at purple
Under outer light irradiation, there is not red fluorescence band in nature controlling line, then test strips be considered as invalid；There is red fluorescence band in nature controlling line, then test strips is considered as
Effectively.When detecting with in-situs tester after chromatography, nature controlling line fluorescence signal intensity be less than 100, then test strips be considered as invalid；Nature controlling line fluorescence signal
Intensity is more than 100, then test strips is considered as effectively；Cefalexin in sample is analyzed dense with the detection line fluorescent bands depth (or fluorescence signal intensity)
Degree, fluorescent bands the deepest (signal intensity is the strongest), then in sample, cefalexin concentration is the lowest.
The present invention detects the fluorescence immune chromatography test strip of cefalexin in animal food and qualitatively or quantitatively detects cefalexin in milk
Residual quantity, sample pretreatment process is simple, after milk sample centrifugal degreasing, can directly measure, and can detect gross sample simultaneously.Assay method
Simply, saving time, whole detection process only needs to complete for 30 minutes.In milk, the lowest detection line of cefalexin residual is 0.16 μ g/L, has
Specificity is high, sensitivity high, can play a significant role in animal food in the residue detection of cefalexin.
Accompanying drawing explanation
Fig. 1 is the examination criteria curve chart of the cefalexin in embodiment 1.
Detailed description of the invention
Embodiment 1: the preparation of cefalexin residual fluorescence immunochromatographydetecting detecting test strip
1. the synthesis of protein-coupled antigen
(1) synthesis of cefalexin immunizing antigen
Use carbodlimide method to carry out coupling cefalexin and bovine serum albumin (BSA) and obtain immunizing antigen.
Concrete operations are as follows: take cefalexin 15mg and be dissolved in 5mL PBS (0.01mol/L, pH7.4), add 25mg carbodiimide (EDC)
Stirring 30 minutes with 10mgN-N-Hydroxysuccinimide (NHS), take BSA10mg and be dissolved in the cefalexin solution of activation, 25 DEG C of stirrings are anti-
Answer 2 hours, then 4 DEG C of reactions are overnight.Under the conditions of 4 DEG C, with PBS 3 days, obtaining cefalexin immunizing antigen, subpackage is frozen.
(2) synthesis of cefalexin envelope antigen
By cefalexin and ovalbumin (OVA), use glutaraldehyde method to carry out coupling and obtain envelope antigen.
Concrete operations are as follows: take cefalexin 10mg and be dissolved in 5mL PBS (0.01mol/L, pH7.4), add 1% glutaraldehyde solution stirring
30 minutes, taking OVA30mg and be dissolved in 1mLPBS, join in the cefalexin solution of activation, 25 DEG C of stirrings are reacted 2 hours, and 4 DEG C were reacted
Night.Under the conditions of 4 DEG C, with PBS 3 days, obtaining cefalexin envelope antigen, subpackage is frozen.
2. the preparation of monoclonal antibody
(1) animal immune
By the Freund's complete adjuvant emulsifying of the immunogen of synthesis with equivalent, lumbar injection 8 week old female BAl BIc/C mice, dosage is 100 μ g/.
Then add incomplete Freund's adjuvant lumbar injection booster immunization 3 times with the dosage of 100 μ g/, be spaced 2 weeks.Little respectively at the most immune latter 10 days
Rathole socket of the eye trailing edge venous blood collection, measures antibody titer.
(2) cell merges and cloning
Take the splenocyte of immune mouse, in 5: 1 ratios and Sp2/0 myeloma cell fusion, prepare hybridoma.Use limiting dilution assay screening energy
The hybridoma cell strain of enough stably excreting cefalexin monoclonal antibodies, liquid nitrogen cryopreservation after amplification culture.
(3) preparation of monoclonal antibody and purification
Using female BAl BIc/C mouse inbred lines, only, within 5 days, pneumoretroperitoneum injects hybridoma 10 to lumbar injection incomplete Freund's adjuvant 0.5mL/6Individual/only,
Take mouse ascites after 7 days, be purified through octanoic acid-saturated ammonium sulfate method, subpackage ,-20 DEG C of preservations.
3. the aldehyde radical of fluorescent nano particle:
Take 2mg fluorescent nano particle (20mg/mL, 100 μ L), with the carbonate buffer solution (pH=9.5) of 25mM wash 3 times (12000r/min,
4 minutes) after be suspended in the 100 above-mentioned carbonate buffer solutions of μ L.Adding the glucosan of 120 μ L aldehyde radicals, mixing, the most ultrasonic, lucifuge is anti-
Answer 3-4 hour (room temperature).After washing 3 times (12000r/min, 4 minutes) with above-mentioned carbonate buffer solution, it is suspended in 100 μ L carbonate buffer
In liquid.1500r/min is centrifuged 1 minute, goes the removal of impurity, is placed on 4 DEG C of standby traget antibodies and is used.
4. the preparation of fluorescent nano particle labelling cefalexin monoclonal antibody:
By 0.5mg cefalexin monoclonal antibody 4 DEG C of dialysed overnight of carbonate buffer solution, mix with the fluorescent nano particle of above-mentioned aldehyde radical, 4 DEG C
Reaction is overnight.Then, adding boron Cyanogran., final concentration of 5mM, 4 DEG C are reacted 2-4 hour.Add isopyknic confining liquid (50mM tris-HCl
PH7.8, containing 2%BSA, 4% sucrose), close 12 hours or overnight；Finally with the antibody 3 that 50mM tris-HCl pH7.8 washing labelling is good
All over (12000r/min, 4 minutes, 4 DEG C), then with 100 μ L50mM tris-HCl pH7.8 (containing 0.9%NaCl, 0.2%BSA, 0.05%
Tween-20) suspending, 4 DEG C of lucifuges store for future use.
5. it is coated with the pad of fluorescent-labeled antibody:
Glass fibre membrane is soaked in 0.2mol/L Tris-HCl (pH7.8), in the treatment fluid of 1.0%TritonX-100,3.0%BSA, in 4 DEG C of bars
Soak 2 hours under part；Then dry 2 hours at 37 DEG C, standby.Glass fibre membrane is cut into the width of 10mm again, then at Bio-Dot XYZ
On 3050 three-dimensional specking platforms, it is sprayed onto glass fibre membrane with the Bio-Jet Quanti300 noncontact quantitation nozzle that declines, dries 1 hour at 37 DEG C, standby
With.
6. detection line and nature controlling line are coated on nitrocellulose filter:
Described detection line is the envelope antigen that cefalexin is prepared with ovalbumin (OVA)；By cefalexin and ovalbumin (OVA), use
Glutaraldehyde method carries out coupling and obtains envelope antigen.Take cefalexin 10mg to be dissolved in 5mL PBS (0.01mol/L, pH7.4), add 1% penta 2
Aldehyde solution stirring 30 minutes, takes OVA30mg and is dissolved in 1mLPBS, joins in the cefalexin solution of activation, 25 DEG C stirring reaction 2 hours, 4 DEG C
Reaction is overnight.Under the conditions of 4 DEG C, with PBS 3 days, obtaining cefalexin envelope antigen, subpackage is frozen.Goat-anti BALB/C mice immune globulin
Bai Kangti (sheep anti-mouse igg) is as antigen, immune sheep with the immunoglobulin of BALB/C mice, extracts the antibody globulin in immune sheep serum
Make.Cefalexin-ovalbumin envelope antigen and goat-anti BALB/C mice immune globulin antibody are adjusted to being coated diluent respectively
0.3-1.0mg/mL, film liquid measure is 25 μ L/25-30cm, they is carried out as detection be sprayed on nitrocellulose filter parallel with nature controlling line of line
Being coated, detection line and nature controlling line interval 4-7mm, then dry 2 hours at 37 DEG C, envelope, in case reagent paper backboard pasting board is used.
7. the preparation of test strips
Once mutual the pasting treated sample pad, be coated with the pad of fluorescent-labeled antibody, be coated with detection line and matter of overlap joint on backboard
The nitrocellulose filter of control line and adsorptive pads, obtain test strips, be cut into 4mm width as requested, seal standby together with desiccant.
On described glass fibre membrane, the diameter range of the fluorescent nano particle of coating is 70nm, the described fluorescent nano particle in said method preparation,
The wave-length coverage that can launch is 600-630nm.
Embodiment 1 prepares the test example of test strips:
Test example 1
The detection of cefalexin residual in sample
(1) sample pre-treatments
Milk sample: take appropriate milk sample, 4 DEG C, 12000r/min, centrifugal 10 minutes, removes upper-layer fat, takes off a layer solution sample 150 μ L
For analyzing.
(2) ELISA test strip is used
In test strips, sampling point directly drips above-mentioned centrifugal degreasing milk sample, after chromatographing 15-20 minute, detects reading with homemade in-situs tester,
Or obtain fluorescence intensity level with Fluorescence Scanner；
(3) Analysis of test results:
By the fluorescence intensity meansigma methods (H) of the detection line of the standard solution of each concentration obtained divided by first standard solution (0 standard)
Detection line fluorescence intensity level (H0) it is multiplied by 100% again, obtain percentage detection line fluorescence intensity level；Computing formula is: detection line percentage fluorescence is strong
Angle value (%)=(H/H0) × 100%；In formula, H is the detection line average fluorescent strength value of standard solution, H0It it is the inspection of 0 μ g/L standard solution
Survey line average fluorescent strength value；With the semilog value of cefalexin standard concentration as X-axis, detection line percentage detection line fluorescence intensity level is Y-axis,
Draw canonical plotting；Calculate the detection line percentage fluorescence intensity level of sample solution by same way, corresponding go out a sample concentration, from mark
The residual quantity of cefalexin in sample is read on directrix curve.
Test example 2
Test strips sensitivity experiment
Measure the zero standard liquid of 20 different batches respectively, calculate mean percent detection line height value (H0) and standard deviation (S), at standard curve
On find H0Mass concentration corresponding for+3S is theoretical Monitoring lower-cut (LOD), and this concentration is sensitivity.
Result shows that the test strips that the present invention develops is limited to 0.09 μ g/L to the lowest detection of cefalexin residual in milk sample.
Test example 3
Test strips specific assay
Selection includes that penicillin similar with cefalexin 26S Proteasome Structure and Function and other 5 class antibiotic measure cross reacting rate.Standard by various medicines
Curve respectively obtains its 50% inhibition concentration.With following formula calculate the test kit cross reacting rate to other medicines:
Cross reacting rate (%)=(causing the analog concentration of the concentration of 50% suppression cefalexin/cause 50% suppression) × 100%.
Result of the test is as shown in table 1, and 6 kinds of medicines of test and cefalexin do not have cross reaction.
The cross reactivity of table 1 cefalexin detection kit
| Medicine name | Cross reacting rate (100%) |
| Cefalexin | 100 |
| Sulfadiazine | <0.01 |
| Chloromycetin | <0.01 |
| Oxytetracycline | <0.01 |
| Gentamycin | <0.01 |
| Erythromycin | <0.01 |
| Penicillin | <0.01 |
Test example 4
Precision, accuracy test
Sample precision test:
1) taking cefalexin standard sample, prepare variable concentrations sample, take each three of the test strips of four different batches respectively, each concentration repeats 4
Secondary, calculate relative standard deviation respectively.Result shows, the relative standard deviation in milk is below 18%.
2) in blank milk, cefalexin titer extremely final concentration of 5 μ g/L, 1.25 μ g/L and 0.16 μ g/L are added.Respectively sample is added
Recovery test, each concentration do 4 parallel, respectively accuracy in computation.Result show milk, tissue sample add accuracy 100-115% it
Between.
Test example 5
Test strips storage life is tested
Test strips preservation condition is room temperature, through the mensuration of 3 months, the maximum absorbance value (zero standard) of test strips, 50% inhibition concentration, head
Cefalexin adds practical measurement value all within normal range.Consider, during transport and using, to have improper preservation condition and occur, by reagent paper
Bar is placed 4 days under conditions of preserving at 37 DEG C, is accelerated senile experiment, and result shows that this test strips indices complies fully with requirement.More than from
Result can show that test strips can at least can preserve more than 3 months at normal temperatures.
Embodiment described above is only to be described the preferred embodiment of the present invention, is not defined the scope of the present invention, is not taking off
On the premise of the present invention designs spirit, various deformation that technical scheme is made by this area ordinary skill technical staff and improvement, all should
Fall in the protection domain that claims of the present invention determines.
Claims (3)
1. a cefalexin residual fluorescence immunochromatographydetecting detecting test strip, it is characterised in that: on backboard, paste treated sample pad, painting successively
Being covered with the pad of fluorescent-labeled antibody, nitrocellulose filter and adsorptive pads, it is cefalexin-ovalbumin that nitrocellulose filter is coated with detection line
Envelope antigen and nature controlling line are goat-anti BALB/C mice immune globulin antibody.Each portion in cefalexin residual fluorescence immunochromatographydetecting detecting test strip
Divide containing following component:
A. pad, applied atop has fluorescent-labeled antibody, described traget antibody to be rare-earth fluorescent nano-particle and cefalexin specific antibody employingization
Learning the conjugate that cross-linking method is attached obtaining, concrete preparation process is:
(1) synthesis of cefalexin-bovine serum albumin immunizing antigen: use carbodlimide method to carry out coupling cefalexin and bovine serum albumin (BSA)
Obtain immunizing antigen.Taking cefalexin 15mg to be dissolved in 5mL phosphate buffer PBS, wherein, in PBS, the concentration of phosphate radical is 0.01mol/L,
The pH of solution is 7.4, adds 25mg carbodiimide (EDC) and 10mg N-hydroxy-succinamide (NHS) stirs 30 minutes, take BSA10mg
Being dissolved in the cefalexin solution of activation, 25 DEG C of stirrings are reacted 2 hours, then 4 DEG C of reactions are overnight.Under the conditions of 4 DEG C, with PBS 3 days,
Obtaining cefalexin immunizing antigen, subpackage is frozen；
(2) animal immune: employing female BAl BIc/C mouse inbred lines is as immune animal, with cefalexin-bovine serum albumin conjugate as immunogen；
(3) cell merges and cloning: take immune balb/c mice splenocyte and Sp2/0 myeloma cell fusion, screens cell strain, obtains stable point
Secrete the hybridoma cell strain of monoclonal antibody；
(4) preparation of monoclonal antibody and purification: use female BAl BIc/C mouse inbred lines, lumbar injection incomplete Freund's adjuvant, pneumoretroperitoneum injection in 5 days
Hybridoma cell strain 106Individual/only, take mouse ascites after 7 days, after being purified, obtain monoclonal antibody subpackage ,-20 DEG C of preservations；
(5) prepared by immunological probe: take fluorescent nano particle carbonate buffer solution pH9.5 centrifuge washing, suspends, and adds the glucosan reaction of aldehyde radical
4h, adds above-mentioned cefalexin monoclonal antibody and mixes 4 DEG C overnight.Add boron sodium cyanide solution in 4 DEG C react 4h, washing, 4 DEG C of storages, standby
With.
B. nitrocellulose filter, is coated with detection line and nature controlling line above, and detection line is that cefalexin uses glutaraldehyde method to carry out with ovalbumin (OVA)
The coating antigen that coupling obtains, nature controlling line is goat-anti BALB/C mice immune globulin antibody (sheep anti-mouse igg), and concrete preparation process is:
(1) synthesis of cefalexin-ovalbumin envelope antigen: by cefalexin and ovalbumin (OVA), uses glutaraldehyde method to carry out coupling and is coated
Antigen.Taking cefalexin 10mg to be dissolved in 5mL phosphate buffer PBS, wherein, in PBS, the concentration of phosphate radical is 0.01mol/L, the pH of solution
It is 7.4, adds 1% glutaraldehyde solution and stir 30 minutes, take OVA30mg and be dissolved in 1mLPBS, join in the cefalexin solution of activation,
25 DEG C of stirrings are reacted 2 hours, and 4 DEG C of reactions are overnight.Under the conditions of 4 DEG C, with PBS 3 days, obtaining cefalexin envelope antigen, subpackage is frozen；
(2) goat-anti BALB/C mice immune globulin antibody (sheep anti-mouse igg) is as antigen with the immunoglobulin of BALB/C mice, immune sheep,
The antibody globulin extracted in immune sheep serum is made；
(3) cefalexin-ovalbumin envelope antigen and goat-anti BALB/C mice immune globulin antibody are adjusted to being coated diluent respectively
0.3-1.0mg/mL, film liquid measure is 25 μ L/25-30cm, and using them, as detecting, line is parallel with nature controlling line to be sprayed on nitrocellulose filter
It is coated, detection line and nature controlling line interval 4-7mm, then dries 2 hours at 37 DEG C.
C. sample pad, is specifically processed as: glass fibre membrane is soaked in 0.2mol/LTris-HCl, and the pH of solution is 7.8,1.0%TritonX-100,
In the treatment fluid of 3.0%BSA, soak 2 hours under the conditions of 4 DEG C；Then dry 2 hours at 37 DEG C, standby.
Test strips the most according to claim 1, it is characterised in that: the wave-length coverage that described fluorescent nano particle is launched is 600-630nm.
3. utilize the method that the cefalexin residual fluorescence immunochromatographydetecting detecting test strip detection cefalexin described in claim 1 remains, its feature
It is: comprise the following steps:
(1) sample pre-treatments: milk sample is centrifuged, and condition is rotating speed 12000r/min, and the time is 10 minutes, directly measures after defat；
(2), with ELISA test strip: the upper sampling point in test strips directly drips above-mentioned centrifugal degreasing milk sample, after chromatographing 15-20 minute, survey at 615nm
Fluorescence intensity level；
(3) Analysis of test results:
With the fluorescence intensity meansigma methods (H) detection divided by first standard solution (0 standard) detecting line of the standard solution of each concentration obtained
Line fluorescence intensity level (H0) it is multiplied by 100% again, obtain percentage detection line fluorescence intensity level；Computing formula is: percentage detection line fluorescence intensity level
(%)=(H/H0) × 100%；In formula, H is the detection line average fluorescent strength value of standard solution, H0The detection line being 0 μ g/L standard solution is flat
All fluorescence intensity levels；With the semilog value of cefalexin standard concentration as X-axis, percentage detection line fluorescence intensity level is Y-axis, draws standard
Curve chart；Calculate the percentage detection line fluorescence intensity level of sample solution by same way, corresponding go out a sample concentration, from standard curve
The residual quantity of cefalexin in upper reading sample.
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| CN103543260A (en) * | 2013-07-30 | 2014-01-29 | 中国检验检疫科学研究院 | Method for detecting biomacromolecule based on magnetic separation-quantum dot immunofluorescence sensing and reagent preparation method |
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| CN103543260A (en) * | 2013-07-30 | 2014-01-29 | 中国检验检疫科学研究院 | Method for detecting biomacromolecule based on magnetic separation-quantum dot immunofluorescence sensing and reagent preparation method |
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