CN103897982B - A kind of device for building anaerobic condition - Google Patents
A kind of device for building anaerobic condition Download PDFInfo
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- CN103897982B CN103897982B CN201410115615.9A CN201410115615A CN103897982B CN 103897982 B CN103897982 B CN 103897982B CN 201410115615 A CN201410115615 A CN 201410115615A CN 103897982 B CN103897982 B CN 103897982B
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- water
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- anaerobic condition
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
This patent discloses a kind of device for building anaerobic condition, this device is primarily of being oxidized rod, copper conduit, alcohol blast burner, digital display screen, integrated electric plate module, minipump, automatically controlled valve, automatically controlling the formations such as threeway, condenser, instrument air dryer, nitrogen gas stream gauge, heat exchanging holes, thermal absorption room, oxidizing chamber, casing, rubber hose.This patent can build anaerobic condition by two kinds of patterns, the first pattern reaches the effect of oxygen in air in the removing finite space by oxygen in the oxidizing reaction consumed cabin air of copper mesh, the second pattern is by the mode exhausted air of vacuum pump suction, nitrogen injection, build anaerobic condition, two kinds of patterns are all passed through the automatic mode controlled and are realized.Relatively traditional deoxygenation and anaerobic condition obtain manner, it is high that this device has tolerance range, effective, consuming time few, easy and simple to handle, efficiency advantages of higher, is applicable to the acquisition of anaerobic environment in soil and other correlative study.
Description
Technical field
This patent relates to a kind of device for building anaerobic condition, and a kind of copper oxidizing reaction that relies on to remove in air oxygen or relies on nitrogen to replace air thus build the device of anaerobic environment specifically.
Background technology
In soil, methane-oxidizing bacteria aerobic oxidation has achieved more achievement in research, and under normal circumstances, the culture condition of the aerobic methane oxidation of soil is easy to realize and control.But also rarely have report about the research of methane-oxidizing bacteria anaerobic oxidation in soil, the research of this one side has become the focus in this field gradually.Under normal circumstances, laboratory study methane oxidation adopts band plug serum bottle to cultivate, and a certain amount of soil is placed in bottle, injects quantitative CH after jam-pack bottle stopper
4, by analyzing CH
4the oxidization condition of the Changeement methane-oxidizing bacteria of concentration.
In soil methane-oxidizing bacteria anaerobic oxidation research in, related experiment equipment can't meet requirement of experiment.In order to simulate the methane oxidation in landfill yard overlying soil under anaerobic condition, be all adopt the method for nitrogen purge gas to build anaerobic condition in current existing research.But adopt the method for nitrogen purge gas to have a lot of shortcoming: the first, the average molecular mass of air is 29g/mol, N
2average molecular mass be 28g/mol, average molecular mass is more or less the same, and just there is N in this case
2with the mixing of air, usual laboratory adopts 5 minutes nitrogen purge gas methods, and this can not ensure that air is got rid of completely; The second, also there is research display, N
2existence may to methane-oxidizing bacteria methane oxidation produce certain influence, if then adopt N
2air-discharging method, the N in culturing bottle
2can have an impact to experimental anaerobic research.
At present, the creation of laboratory anaerobic culture conditions is substantially all depend on anaerobic jar, anaerobic reaction bag and anaerobic culture box, but these equipment all can not meet the requirement of this research to a large amount of batch experiments, the research of namely for methane oxidation bacteria anaerobic oxidation is usually directed to more control condition, parallel group must be comprised again, in this case, culturing bottle quantity can reach about 40-150 usually, therefore culture tank, reaction bag and incubator can not meet requirement of experiment at all.
Summary of the invention
For solving above-mentioned technical disadvantages, this patent provides a kind of anaerobic condition constructing device, this device is running operational mode once by automatically controlling the oxidation of copper mesh and the keying of alcohol blast burner in the opening and closing time of minipump, oxidation rod, achieve the deoxygenation to air in the finite space in culturing bottle, operational mode two times by automatically controlling minipump and nitrogen gas stream gauge valve opening and closing time, achieve the injection of discharge to culturing bottle air and nitrogen.Relatively traditional deoxygenation and anaerobic condition obtain manner, it is high that this device has tolerance range, effective, consuming time few, easy and simple to handle, efficiency advantages of higher, is applicable to the acquisition of anaerobic environment in soil and other correlative study.
This patent adopts following technical scheme for achieving the above object:
For building a device for anaerobic condition, wherein: be provided with oxidation rod, ceramic adapter bonnet, copper mesh, silicon nitride ceramics shell, copper conduit, ceramics bracket, alcohol blast burner, water of condensation water inlet pipe, glass curved tube, water of condensation rising pipe, integrated electric plate module, minipump, automatically controlled valve in casing, automatically control threeway, condenser, instrument air dryer, sponge, calcium chloride powder, nitrogen inlet, nitrogen gas stream gauge, culturing bottle, threeway, heat exchanging holes, thermal absorption room, oxidizing chamber, rubber hose, culturing room's side door, described apparatus main body shell is cuboid, and oxidizing chamber top cover is connected with casing by set screw nut, and oxidizing chamber top cover is provided with heat exchanging holes, culturing room's side door is connected with casing by rotating shaft, the side of casing is provided with air outlet, box house is separated into 9 cells, be respectively thermal absorption room, oxidizing chamber, the front condensing chamber of glass curved tube and rear condensing chamber are housed, the canyon of minipump is housed, the kiln of instrument air dryer is housed, the watch-keeping cubicle of integrated electric plate module is housed, the load chamber of culturing bottle is housed and the measuring room of nitrogen gas stream gauge is housed, casing is relied on to separate between 9 cells, wherein thermal absorption room and oxidizing chamber, canyon and front condensing chamber, front condensing chamber and oxidizing chamber, oxidizing chamber and rear condensing chamber, rear condensing chamber and kiln, kiln and measuring room are all connected by copper conduit between any two, kiln and load chamber, all connected by rubber hose between threeway, the overall mode by welding of digital display screen is connected to casing, copper conduit, water of condensation water inlet pipe, water of condensation rising pipe and nitrogen inlet all adopt screw to be fixed in casing, water of condensation water inlet pipe is connected with water tap by the side of rubber hose through casing, and water of condensation rising pipe is connected with extraneous collector by the side of rubber hose through casing, nitrogen inlet duct is connected with nitrogen gas storage tank by rubber hose, oxidizing chamber forms by being oxidized rod, ceramics bracket and alcohol blast burner, and ceramics bracket is arranged on the bottom of oxidation rod for playing a supportive role, condenser is made up of water of condensation water inlet pipe, water of condensation rising pipe and glass curved tube, instrument air dryer, by sponge and calcium chloride powder constituent, is provided with sponge and calcium chloride powder in instrument air dryer, minipump and alcohol blast burner are all fixed on the bottom of room, place.
The oxidation rod of described device wraps up copper mesh by silicon nitride ceramics shell and forms, in spheroid shape, two ends arrange the adapter bonnet of silicon nitride ceramic material, in operational mode once, air is imported by adapter bonnet, the oxidizing reaction that rod completes copper mesh is oxidized by alcohol blast burner heat, thus oxygen depleted, the air of deoxygenation is discharged from the other end by adapter bonnet.
The main body cover material of described device adopts 316 type stainless steels, and oxidizing chamber inner surface material adopts Si
3n
4pottery, the propping material of oxidizing chamber adopts A3 or 45# structural section.
The water of condensation water inlet pipe and water outlet pipe of described device adopts the netted engineering plastics pipe of PP-R crosslinked polypropylene high-density, and the sponge in instrument air dryer adopts pore sponge, and the air lead bacterium connecting formant adopts copper fine duct.
The cold water of the oxidation rod of described device, sponge, calcium chloride powder and thermal absorption room can be changed.
The valvular keying of described device is all controlled by integrated electric plate module, and opening and closing time is determined according to the volume of culturing bottle and the flow of nitrogen.
Described device, under the first operational mode, is aspirated by vacuum pump, and the air in culturing bottle crosses copper mesh, oxygen gas component is consumed, culturing bottle under vacuum conditions can air after active absorption deoxidation, thus achieves the circulation of oxygen free air in whole service system, reaches the object of deoxygenation; Under the second operational mode, by setting vacuum pump suction time, realize the extraction to air in culturing bottle, then by controlling the injection length of nitrogen, reach the requirement to filling nitrogen in culturing bottle.
Described device is operational mode two times, by controlling the keying of minipump thus controlling the rate of air sucked in required of air, by the charge flow rate of nitrogen gas stream gauge adjustment nitrogen, controlled the inlet period of nitrogen by the automatically controlled valve in inlet pipe, air inlet is complete, the threeway on manual-lock culturing bottle.
This patent has following beneficial effect:
1, in operation operational mode once, adopt in the product of copper mesh oxidation deoxygenation and do not produce gas, avoid assorted gas in deoxidation air and produce the impact on experiment.
2, by the automatic open close of automatic control mode operated valve, strictly can accurately control the tonifying Qi time of bleeding, decrease the error in experiment.
3, decrease the manually-operated time, improve preparation efficiency.
4, provide two kinds of alternative models, can select according to actual needs.
5, the construction of anaerobic condition under the larger amt culturing bottle of laboratory is applicable to.
Accompanying drawing explanation
Fig. 1 is the internal structure schematic diagram of this patent.
Fig. 2 is this patent stereoscopic structural representation.
Fig. 3 is the structural representation of oxidation rod 1 in this patent.
In figure: oxidation rod 1, pottery adapter bonnet 2, copper mesh 3, silicon nitride ceramics shell 4, copper conduit 5, ceramics bracket 6, alcohol blast burner 7, oxidizing chamber top cover 8, set screw nut 9, digital display screen 10, water of condensation water inlet pipe 11, glass curved tube 12, water of condensation rising pipe 13, integrated electric plate module 14, minipump 15, automatically controlled valve 16, automatic control threeway 17, condenser 18, instrument air dryer 19, sponge 20, calcium chloride powder 21, nitrogen inlet 22, nitrogen gas stream gauge 23, culturing bottle 24, threeway 25, heat exchanging holes 26, thermal absorption room 27, oxidizing chamber 28, casing 29, air outlet 30, rubber hose 31, culturing room's side door 32.
Embodiment
Below this patent and embodiment thereof are described in further detail.
Embodiment 1, a kind of device for building anaerobic condition, wherein: in casing 29, be provided with oxidation rod 1, pottery adapter bonnet 2, copper mesh 3, silicon nitride ceramics shell 4, copper conduit 5, ceramics bracket 6, alcohol blast burner 7, water of condensation water inlet pipe 11, glass curved tube 12, water of condensation rising pipe 13, integrated electric plate module 14, minipump 15, automatically controlled valve 16, automatic control threeway 17, condenser 18, instrument air dryer 19, sponge 20, calcium chloride powder 21, nitrogen inlet 22, nitrogen gas stream gauge 23, culturing bottle 24, threeway 25, heat exchanging holes 26, thermal absorption room 27, oxidizing chamber 28, rubber hose 31, culturing room's side door 32, described apparatus main body shell is cuboid, and oxidizing chamber top cover 8 is connected with casing 29 by set screw nut 9, and oxidizing chamber top cover 8 is provided with heat exchanging holes 26, culturing room's side door 32 is connected with casing 29 by rotating shaft, the side of casing 29 is provided with air outlet 30, casing 29 inside is separated into 9 cells, be respectively thermal absorption room 27, oxidizing chamber 28, the front condensing chamber of glass curved tube 12 and rear condensing chamber are housed, the canyon of minipump 15 is housed, the kiln of instrument air dryer 19 is housed, the watch-keeping cubicle of integrated electric plate module 14 is housed, the load chamber of culturing bottle 24 is housed and the measuring room of nitrogen gas stream gauge 23 is housed, casing 29 is relied on to separate between 9 cells, wherein thermal absorption room 27 and oxidizing chamber 28, canyon and front condensing chamber, front condensing chamber and oxidizing chamber 28, oxidizing chamber 28 and rear condensing chamber, rear condensing chamber and kiln, kiln and measuring room are all connected by copper conduit between any two, kiln and load chamber, all connected by rubber hose 31 between threeway 25, the overall mode by welding of digital display screen 10 is connected to casing 29, copper conduit 5, water of condensation water inlet pipe 11, water of condensation rising pipe 13 and nitrogen inlet 22 all adopt screw to be fixed in casing 29, water of condensation water inlet pipe 11 is connected with water tap by the side of rubber hose 31 through casing 29, and water of condensation rising pipe 13 is connected with extraneous collector by the side of rubber hose 31 through casing 29, nitrogen inlet duct 22 is connected with nitrogen gas storage tank by rubber hose 31, oxidizing chamber 28 is made up of oxidation rod 1, ceramics bracket 6 and alcohol blast burner 7, and ceramics bracket 6 is arranged on the bottom of oxidation rod 1 for playing a supportive role, condenser 18 is made up of water of condensation water inlet pipe 11, water of condensation rising pipe 13 and glass curved tube 12, instrument air dryer 19 is made up of sponge 20 and calcium chloride powder 21, is provided with sponge 20 and calcium chloride powder 21 in instrument air dryer 19, minipump 15 and alcohol blast burner 7 are all fixed on the bottom of room, place.
Embodiment 2, a kind of device for building anaerobic condition, wherein: oxidation rod 1 wraps up copper mesh 3 by silicon nitride ceramics shell 4 and forms, in spheroid shape, two ends arrange ceramic adapter bonnet 2,, import air by ceramic adapter bonnet 2 in operational mode once, be oxidized by alcohol blast burner 7 heat the oxidizing reaction that rod 1 completes copper mesh 3, thus oxygen depleted, the air of deoxygenation is discharged from the other end by ceramic adapter bonnet 2.All the other are with embodiment 1.
Embodiment 3, a kind of device for building anaerobic condition, wherein: the warm air after deoxygenation carries out heat exchange by the Tortuous flow in the glass curved tube 12 of condenser 18 and cold water, reach the effect of condensation air, all be provided with condenser 18 in the import and export of oxidizing chamber 28, thus prevent water vapour to reflux the harm caused other component.All the other are with embodiment 1 or 2.
Embodiment 4, a kind of device for building anaerobic condition, wherein: the air after deoxygenation is dewatered by the carrying out of the calcium chloride powder 21 of 19 li, moisture eliminator.All the other are with embodiment 1.
Embodiment 5, a kind of device for building anaerobic condition, wherein: operational mode two times, by controlling the keying of minipump 15 thus controlling the rate of air sucked in required of air, the charge flow rate of nitrogen is adjusted by nitrogen gas stream gauge 23, the inlet period of nitrogen is controlled by the automatically controlled valve 16 in inlet pipe, air inlet is complete, the threeway 25 on manual-lock culturing bottle 24.All the other are with embodiment 1.
Embodiment 6, a kind of device for building anaerobic condition, wherein: the top cover 8 of oxidizing chamber 28 is provided with bar shaped heat exchanging holes 26 and lowers the temperature for discharging warm air in overtemperature situation, thermal absorption room 27 communicates with oxidizing chamber 28, thermal absorption room 27 built with cold water for absorbing the unnecessary heat of oxidizing chamber 28.All the other are with embodiment 1 or 3.
Embodiment 7, a kind of device for building anaerobic condition, wherein: minipump 15, automatically controlled valve 16 and the automatic keying controlling threeway 17 are controlled by electroplax module 14, and opening and closing time is determined according to the volume of culturing bottle 24 and the flow of nitrogen.All the other are with embodiment 1 or 5.
Embodiment 8, a kind of device for building anaerobic condition, wherein: casing 29 pushes up upper loading one piece of digital display screen 10 configuration adjustment keyboard; The opening time of minipump 15 and the inlet period of nitrogen are configured by digital display screen 10.All the other are with embodiment 1 or 5 or 7.
Embodiment 9, a kind of device for building anaerobic condition, wherein: the material of casing 29 adopts 316 type stainless steels, oxidizing chamber 28 inner surface material adopts Si
3n
4pottery, the material of the ceramics bracket 6 of oxidizing chamber 28 adopts A3 or 45# structural section.All the other are with implementing 1 or 8.
Embodiment 10, a kind of device for building anaerobic condition, wherein: water of condensation water inlet pipe 11, water of condensation rising pipe 13 all adopt the netted engineering plastics pipe of PP-R crosslinked polypropylene high-density, sponge 20 in instrument air dryer 19 adopts pore sponge, and the air lead connecting formant all adopts copper fine duct.All the other are with embodiment 1 or 4.
Embodiment 11, a kind of device for building anaerobic condition, wherein: the cold water in oxidation rod 1, sponge 20, calcium chloride powder 21 and thermal absorption room 27 can be changed.All the other are with any one in embodiment 1-4.All the other are with embodiment 1 or 2 or 4 or 6 or 10.
Operational mode one, two times, the opening time calculation formula of minipump 15 is as follows:
In operation operational mode once, the opening time of minipump 15 calculates by formula (1):
t = k（nV-
1+V-
2）/q （1）
Wherein: V-
1culturing bottle volume (ml); N culturing bottle quantity; Q vacuum pump suction flow (ml/s); T vacuum pump suction time (s); V-
2the volume (ml) of existing air in=system; K is variation factor, and the value range of k gets 2 ~ 2.5.
Running operational mode two times, the opening time of minipump 15 calculates by formula (1) method, and wherein the value range of k gets 1.2 ~ 2.0.The injection length (i.e. the time of nitrogen flow by-pass valve control unlatching) of N2 calculates by formula (2):
t
1= α（nV-
1+V-
2）/q （2）
Wherein: V-
1culturing bottle volume (ml); N culturing bottle quantity; q
1the flow (ml/s) of nitrogen gas stream gauge display; Injection length (i.e. the time of the nitrogen flow by-pass valve control unlatching) t of N2
1; α is variation factor, and the value range of α gets 1.1 ~ 1.2.
Above variation factor k and α is recorded by test, in order to ensure suction, oxidation, inject effect and introduce.
Under the larger amt culturing bottle of laboratory, the construction of anaerobic condition adopts this equipment.Described device under the first operational mode, by regulating keyboard configuration minipump 15 suction time t, in predetermined suction time t, in culturing bottle, air is aspirated by minipump 15, by being oxidized rod 1, is oxidized excellent 1 liner copper mesh 3, under the high temperature action of alcohol blast burner 7, pass through Si
3n
4the thermal conduction of ceramic package 4, copper mesh 3 reaches the minimum temperature of oxidation, and oxidizing reaction occurs on its surface, consumes the oxygen in institute's suction air, reaches the effect of deoxygenation, be i.e. the culture condition of anaerobism.
Claims (7)
1. one kind for building the device of anaerobic condition, it is characterized in that: in casing (29), be provided with oxidation rod (1), pottery adapter bonnet (2), copper mesh (3), silicon nitride ceramics shell (4), copper conduit (5), ceramics bracket (6), alcohol blast burner (7), water of condensation water inlet pipe (11), glass curved tube (12), water of condensation rising pipe (13), integrated electric plate module (14), minipump (15), automatically controlled valve (16), automatic control threeway (17), condenser (18), instrument air dryer (19), sponge (20), calcium chloride powder (21), nitrogen gas stream gauge (23), culturing bottle (24), threeway (25), heat exchanging holes (26), thermal absorption room (27), oxidizing chamber (28), rubber hose (31), culturing room's side door (32), described apparatus main body shell is cuboid, and oxidizing chamber top cover (8) is connected with casing (29) by set screw nut (9), and oxidizing chamber top cover (8) is provided with heat exchanging holes (26), culturing room's side door (32) is connected with casing (29) by rotating shaft, the side of casing (29) is provided with water of condensation water inlet pipe (11), water of condensation rising pipe (13) and air outlet (30), casing (29) inside is separated into 9 cells, be respectively thermal absorption room (27), oxidizing chamber (28), the front condensing chamber of glass curved tube (12) and rear condensing chamber are housed, the canyon of minipump (15) is housed, the kiln of instrument air dryer (19) is housed, the watch-keeping cubicle of integrated electric plate module (14) is housed, the load chamber of culturing bottle (24) is housed and the measuring room of nitrogen gas stream gauge (23) is housed, casing (29) is relied on to separate between 9 cells, wherein thermal absorption room (27) and oxidizing chamber (28), canyon and front condensing chamber, front condensing chamber and oxidizing chamber (28), oxidizing chamber (28) and rear condensing chamber, rear condensing chamber and kiln, kiln and measuring room are all connected by copper conduit between any two, kiln and load chamber, all connected by rubber hose (31) between threeway (25), the overall mode by welding of digital display screen (10) is connected on casing (29), copper conduit (5), water of condensation water inlet pipe (11), water of condensation rising pipe (13) and nitrogen inlet (22) all adopt screw to be fixed in casing (29), water of condensation water inlet pipe (11) is connected with water tap by the side of rubber hose (31) through casing (29), and water of condensation rising pipe (13) is connected with extraneous collector by the side of rubber hose (31) through casing (29), nitrogen inlet duct (22) is connected with nitrogen gas storage tank by rubber hose (31), oxidizing chamber (28) is made up of oxidation rod (1), ceramics bracket (6) and alcohol blast burner (7), and the bottom that ceramics bracket (6) is arranged on oxidation rod (1) is used for playing a supportive role, condenser (18) is made up of water of condensation water inlet pipe (11), water of condensation rising pipe (13) and glass curved tube (12), instrument air dryer (19) is made up of sponge (20) and calcium chloride powder (21), is provided with sponge (20) and calcium chloride powder (21) in instrument air dryer (19), minipump (15) and alcohol blast burner (7) are all fixed on the bottom of room, place, oxidation rod (1) is made up of copper mesh (3), ceramic adapter bonnet (2) and silicon nitride ceramics shell (4), and copper mesh (3) is enclosed in silicon nitride ceramics shell (4), , import air by ceramic adapter bonnet (2) in operational mode once, be oxidized by alcohol blast burner (7) heat the oxidizing reaction that rod (1) completes copper mesh (3), thus oxygen depleted, the air of deoxygenation is discharged from the other end by ceramic adapter bonnet (2).
2. a kind of device for building anaerobic condition according to claims 1, it is characterized in that: the warm air after deoxygenation carries out heat exchange by the Tortuous flow in the glass curved tube (12) of condenser (18) and cold water, reach the effect of condensation air, all be provided with condenser (18) in the import and export of oxidizing chamber (28), thus prevent water vapour to reflux the harm caused other component.
3. a kind of device for building anaerobic condition according to claims 1, is characterized in that: the carrying out that the air after deoxygenation passes through the inner calcium chloride powder (21) of moisture eliminator (19) dewaters.
4. a kind of device for building anaerobic condition according to claims 1, it is characterized in that: operational mode two times, by controlling the keying of minipump (15) thus controlling the rate of air sucked in required of air, by the charge flow rate of nitrogen gas stream gauge (23) adjustment nitrogen, the inlet period of nitrogen is controlled by the automatically controlled valve (16) in inlet pipe, air inlet is complete, the threeway (25) on manual-lock culturing bottle (24).
5. a kind of device for building anaerobic condition according to claims 1, it is characterized in that: the top cover (8) of oxidizing chamber (28) is provided with bar shaped heat exchanging holes (26) and lowers the temperature for discharging warm air in overtemperature situation, thermal absorption room (27) communicates with oxidizing chamber (28), thermal absorption room (27) built with cold water for absorbing the unnecessary heat of oxidizing chamber (28).
6. a kind of device for building anaerobic condition according to claims 1 or 4, is characterized in that: minipump (15), automatically controlled valve (16) and the keying automatically controlling threeway (17) are controlled by electroplax module (14).
7. a kind of device for building anaerobic condition according to claims 1 or 4, is characterized in that: loading one piece of digital display screen (10) configuration adjustment keyboard on casing (29) top; The opening time of minipump (15) and the inlet period of nitrogen are configured by digital display screen (10).
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| CN110127172B (en) * | 2019-06-10 | 2020-07-10 | 东阳市中振永磁有限公司 | Neodymium iron boron magnetic powder storage method |
| CN112903830A (en) * | 2019-11-19 | 2021-06-04 | 上海交通大学 | Test device and method for measuring oxidation rate of methane in rice field soil |
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