CN103897699B - Polypeptide-quantum dot nano complex solution and synthetic method thereof - Google Patents
Polypeptide-quantum dot nano complex solution and synthetic method thereof Download PDFInfo
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- 239000002096 quantum dot Substances 0.000 title claims abstract description 62
- 238000010189 synthetic method Methods 0.000 title claims abstract description 13
- 239000002243 precursor Substances 0.000 claims abstract description 84
- 229910021642 ultra pure water Inorganic materials 0.000 claims abstract description 40
- 239000012498 ultrapure water Substances 0.000 claims abstract description 40
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 36
- 229920001184 polypeptide Polymers 0.000 claims abstract description 27
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 27
- PJUIMOJAAPLTRJ-UHFFFAOYSA-N monothioglycerol Chemical compound OCC(O)CS PJUIMOJAAPLTRJ-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229940035024 thioglycerol Drugs 0.000 claims abstract description 23
- 238000010438 heat treatment Methods 0.000 claims abstract description 21
- PORWMNRCUJJQNO-UHFFFAOYSA-N tellurium atom Chemical compound [Te] PORWMNRCUJJQNO-UHFFFAOYSA-N 0.000 claims abstract description 13
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims abstract description 13
- 229960001763 zinc sulfate Drugs 0.000 claims abstract description 13
- 229910000368 zinc sulfate Inorganic materials 0.000 claims abstract description 13
- 229910000033 sodium borohydride Inorganic materials 0.000 claims abstract description 12
- 239000012279 sodium borohydride Substances 0.000 claims abstract description 12
- 150000001875 compounds Chemical class 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 238000001308 synthesis method Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 abstract 1
- 238000002360 preparation method Methods 0.000 description 27
- 230000015572 biosynthetic process Effects 0.000 description 22
- 238000003786 synthesis reaction Methods 0.000 description 22
- 238000000034 method Methods 0.000 description 12
- 229910052751 metal Inorganic materials 0.000 description 10
- 239000002184 metal Substances 0.000 description 10
- 238000006243 chemical reaction Methods 0.000 description 7
- 238000002189 fluorescence spectrum Methods 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 4
- 239000000523 sample Substances 0.000 description 4
- 239000008346 aqueous phase Substances 0.000 description 3
- 230000008901 benefit Effects 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000003384 imaging method Methods 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 230000035484 reaction time Effects 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- UHYPYGJEEGLRJD-UHFFFAOYSA-N cadmium(2+);selenium(2-) Chemical compound [Se-2].[Cd+2] UHYPYGJEEGLRJD-UHFFFAOYSA-N 0.000 description 2
- 230000002349 favourable effect Effects 0.000 description 2
- 235000011187 glycerol Nutrition 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 239000004065 semiconductor Substances 0.000 description 2
- 229910004613 CdTe Inorganic materials 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- -1 boron Sodium hydride Chemical compound 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 238000010382 chemical cross-linking Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 238000000799 fluorescence microscopy Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000004005 microsphere Substances 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 239000004054 semiconductor nanocrystal Substances 0.000 description 1
- 229910000077 silane Inorganic materials 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Abstract
The present invention provides the synthetic method of a peptide species quantum dot nano complex solution, and it comprises the steps: one, weighs caddy, thioglycerol and be dissolved in ultra-pure water, regulates solution ph 9 10, obtains the first precursor storing solution；Two, weigh zinc sulfate, thioglycerol and zinc sulfate, thioglycerol are dissolved in ultra-pure water, regulating solution ph 9 10, obtain the second precursor storing solution；Three, weigh polypeptide and polypeptide is dissolved in ultra-pure water, obtaining the 3rd precursor storing solution；Four, weigh tellurium powder, sodium borohydride and tellurium powder, sodium borohydride are dissolved in ultra-pure water, standing, obtain the 4th precursor storing solution；Five, take the first precursor storing solution, add the second precursor storing solution, the 3rd precursor storing solution and the 4th precursor storing solution of different volumes, then final mixed liquor heating is cooled to room temperature, i.e. obtains polypeptide quantum dot nano complex solution.Present invention also offers a peptide species quantum dot nano complex solution.
Description
Technical field
The present invention relates to the synthetic method of one peptide species-quantum dot nano compound, be specifically related to a peptide species-
One step aqueous synthesis method of quantum dot nano complex solution.
Background technology
Quantum dot is made up of hundreds and thousands of atoms, particle diameter at the semiconductor nanocrystals grain of 1 ~ 100nm,
Exciting light can be accepted and produce fluorescence, and the wavelength launching light is controlled, extensively should as bioprobe
In the subject such as molecular biology, medical diagnostics.
(the China such as at present, the synthesis of quantum dot is divided into high temperature oil phase synthesi and aqueous phase synthesis method, Pang Daiwen
Patent No.: ZL200510120546.1) use high temperature organic procedures to be prepared for CdSe quantum dot, Han He
Friends etc. (Chinese Patent Application No.: 200510019940.6) use Aqueous phase to synthesize CdSe quantum dot.
Song Zhenwei etc. (Chinese Patent Application No.: 200810032389.2) disclose a kind of silane parcel semiconductor amount
The method of son point, Sun Kang etc. (Chinese Patent Application No.: 200810032996.9) discloses a kind of water-soluble
The hydrothermal preparing process of ZnCdSe quantum dot, stores up (Chinese Patent Application No.: 200410016424.3) such as Mao Quan
Disclose the preparation method of a kind of quantum dot microsphere being used as biomedical fluorescence probe, tight elegant equality (China
Number of patent application: 200710150113.X) disclose the aqueous phase of a kind of cyclodextrin modified CdTe quantum
Preparation method, but in order to build nano-probe based on quantum dot, need the quantum dot surface in synthesis even
The biomolecule (such as: polypeptide, nucleic acid, antibody etc.) of connection target.Therefore, nano-probe based on quantum dot
Generally require and could be realized by two steps, i.e. synthesize and modify.Modification operation complexity, wastes time and energy,
Strongly limit nano-probe based on quantum dot in the popularization of biological field and application.
The preparation method of polypeptide-quantum dot nano compound, is mainly undertaken in two steps, first synthesizes quantum at present
Point, then utilize the modes such as chemical crosslinking, physical absorption polypeptide is assembled into quantum dot surface formed polypeptide-
Quantum dot nano compound.Process is complicated, and particularly cross-linking process often results in quantum dot light emitting performance loss.
Based on case above, it is necessary to study an a kind of step and realize synthesizing new with the quantum dot synthesis in water of modification
Method, and combine metal bath heating and realize a step of polypeptide functional quantum point and prepare.
Summary of the invention
The technical problem to be solved is to provide a kind of method simplicity, reaction condition gentleness, reappearance
The synthetic method of the polypeptide-quantum dot complex solution of the Wavelength tunable good, single dispersing is good and polypeptide-quantum
Point complex solution.
The technical problem solving the present invention is adopted the technical scheme that: provide one peptide species-quantum dot nano multiple
The synthetic method of polymer solution, described synthetic method comprises the steps:
Step one, weigh caddy, thioglycerol and caddy, thioglycerol be dissolved in ultra-pure water,
Regulation solution ph 9-10, obtains the first precursor storing solution；
Step 2, weigh zinc sulfate, thioglycerol and zinc sulfate, thioglycerol be dissolved in ultra-pure water,
Regulation solution ph 9-10, obtains the second precursor storing solution；
Step 3, weigh polypeptide and polypeptide is dissolved in ultra-pure water, obtaining the 3rd precursor storing solution；
Step 4, weigh tellurium powder, sodium borohydride and tellurium powder, sodium borohydride be dissolved in ultra-pure water, standing,
Obtain the 4th precursor storing solution；
Step 5, take the first precursor storing solution, add the second precursor storing solution of different volumes, add not
3rd precursor storing solution of same volume, is eventually adding the 4th precursor storing solution of different volumes, is mixed by final
Close liquid heating and be then cooled to room temperature, i.e. obtain polypeptide-quantum dot nano complex solution.
Preferably, in step 4, time of repose is 12 hours.
Preferably, in step 5, heating condition is: 90-100 DEG C of heating in constent temperature heater, add
The heat time is 1 hour to 48 hours.
Present invention also offers polypeptide-quantum dot nano prepared by a kind of synthetic method in accordance with the above multiple
Polymer solution..
Compared with prior art, due to the fact that and synchronize to have carried out synthesis and modified in quantum dot building-up process,
Can avoid synthesis in water quantum dot modification again produces the problems such as quantum dot fluorescence reduction, use constant temperature
Heating, has that method is easy, reaction condition is controlled, the time is short, high repeatability and other advantages, and product is by many
Kind of element composition, only need to change the ratio of element and the reaction time just can synthesize the quantum dot of difference fluorescence.
Have greatly at aspects such as molecular biology, cell biology, medical diagnostics and biomedical living imagings
Application prospect.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the invention will be further described, in accompanying drawing:
Fig. 1 is the emission spectrum of the quantum dot that embodiment of the present invention 1-6 obtains；
Fig. 2 is the quantum dot that obtains of embodiment of the present invention 1-6 photo under ultraviolet light irradiates；
Fig. 3 be the quantum dot that obtains of the embodiment of the present invention 6 with cell incubation after imaging results figure.
Detailed description of the invention
In order to make the purpose of the present invention, technical scheme and advantage clearer, below in conjunction with accompanying drawing and reality
Execute example, the present invention is further elaborated.Only should be appreciated that specific embodiment described herein
Only in order to explain the present invention, it is not intended to limit the present invention.
The present invention provides one peptide species-quantum dot nano complex solution, by ratio and the reaction of component
The transmitting wavelength of Timing quantum dot, regulates and controls the character of quantum dot by the kind of polypeptide.By by polypeptide,
Cd ion, Zn ion, Te ion are simultaneously introduced reaction system, use heated at constant temperature in having oxygen atmosphere
One-step synthesis polypeptide-quantum dot nano complex solution.
The invention provides a kind of method simplicity, reaction condition gentleness, favorable reproducibility, polypeptide that single dispersing is good-
The synthetic method of quantum dot complex solution.The simple synthetic method of this quantum dot, product is by multiple element
Composition, the ratio that only need to change element just can synthesize from the different fluorescence of visible near-infrared with the reaction time
Quantum dot, a step can realize the conjunction of quantum dot by adding the polypeptide of difference in functionality in reaction system simultaneously
Become with peptide modified.
The synthetic method of one peptide species-quantum dot nano complex solution that the present invention provides, including walking as follows
Rapid:
Step one, weigh caddy 0.03 ~ 0.4g, thioglycerol 0.05 ~ 1.2g and its solution is dissolved in 100ml
Ultra-pure water in, regulation solution pH value to pH value 9-10, obtain the first precursor storing solution；
Step 2, weigh zinc sulfate 0.03 ~ 0.06g, thioglycerol 0.02 ~ 0.1g, and it is dissolved in 10ml
In ultra-pure water, regulation solution ph, to pH value 9-10, obtains the second precursor storing solution；
Step 3, weigh polypeptide 1-10mg, and be dissolved in 1ml ultra-pure water, obtain the 3rd precursor storage
Standby liquid；
Step 4, weigh tellurium powder 0.01 ~ 0.04g, sodium borohydride 0.025 ~ 0.08g, and be dissolved in 1ml
In ultra-pure water, stand 12 hours, obtain the 4th precursor storing solution；
Step 5, take the first precursor storing solution of 1ml, add the second precursor storing solution of 0.01 ~ 0.1ml,
Add the 3rd precursor storing solution of 0.01 ~ 0.05ml, be eventually adding the 4th precursor deposit of 0.01 ~ 0.05ml
Liquid, in being placed on heated at constant temperature metal bath (constant temperature heating device), arranges synthesis condition and is 90 ~ 100 DEG C and adds
Heat 1 hour to 48 hours, then drops to room temperature, i.e. obtains polypeptide-quantum dot nano complex solution.
The heated at constant temperature metal bath (constant temperature heating device) used is to combine microcomputerized control and semiconductor system
Refrigeration technique manufactures a heated at constant temperature instrument, has intensification quickly, uniformly, and temperature-controllable, favorable reproducibility
Feature.
Embodiment 1:
Step one, the preparation of the first precursor storing solution (precursor storing solution A): weigh caddy 0.03g, sulphur
For glycerine 0.05g, being dissolved in 100ml ultra-pure water, regulation solution ph is to 9, standby.
Step 2, the preparation of the second precursor storing solution (precursor storing solution B): weigh zinc sulfate 0.03g, sulphur
For glycerine 0.02g, being dissolved in 10ml ultra-pure water, regulation solution ph is to 9, standby.
Step 3, the preparation of the 3rd precursor storing solution (precursor storing solution C): weigh polypeptide 10mg, dissolve
In 1ml ultra-pure water, standby.
Step 4, the preparation of the 4th precursor storing solution (precursor storing solution D): weigh tellurium powder 0.040g, boron
Sodium hydride 0.08g, is dissolved in 1ml ultra-pure water, stands 12 hours, standby.
The synthesis of step 5, polypeptide-quantum dot compound: take 1ml precursor storing solution A, adds 0.1ml's
Precursor storing solution B, adds the precursor storing solution C of 0.05ml, is eventually adding the precursor storing solution of 0.01ml
D, is placed in heated at constant temperature metal bath (constant temperature heating device), and arranging synthesis condition is 100 DEG C of heating 1
Hour (h), then cools to room temperature, i.e. obtains the polypeptide required for the present invention-quantum dot complex solution.
Its transmitting wavelength is near 530nm, Fig. 1 and Fig. 2 be shown in by its fluorescence spectrum and photo.
Embodiment 2:
Step one, the preparation of precursor storing solution A: weigh caddy 0.04g, thioglycerol 0.1g, dissolve
In 100ml ultra-pure water, regulation solution ph is to 10, standby.
Step 2, the preparation of precursor storing solution B: weigh zinc sulfate 0.05g, thioglycerol 0.08g, dissolve
In 10ml ultra-pure water, regulation solution ph is to 10, standby.
Step 3, the preparation of precursor storing solution C: weigh polypeptide 4mg, be dissolved in 1ml ultra-pure water, standby
With.
Step 4, the preparation of precursor storing solution D: weigh tellurium powder 0.01g, sodium borohydride 0.025g, dissolve
In 1ml ultra-pure water, stand 12 hours, standby.
The synthesis of step 5, polypeptide-quantum dot compound: take 1ml precursor storing solution A, adds 0.04ml
Precursor storing solution B, add the precursor storing solution C of 0.02ml, be eventually adding the precursor deposit of 0.05ml
Liquid D, in being placed on heated at constant temperature metal bath (constant temperature heating device), arranges synthesis condition and is 100 DEG C and adds
Hot 6h, then cools to room temperature, i.e. obtains the polypeptide required for the present invention-quantum dot complex solution.Its
Transmitting wavelength is near 550nm, Fig. 1 and Fig. 2 be shown in by its fluorescence spectrum and photo.
Embodiment 3:
Step one, the preparation of precursor storing solution A: weigh caddy 0.1g, thioglycerol 0.2g, be dissolved in
In 100ml ultra-pure water, regulation solution ph is to 9, standby.
Step 2, the preparation of precursor storing solution B: weigh zinc sulfate 0.03g, thioglycerol 0.07g, dissolve
In 10ml ultra-pure water, regulation solution ph is to 9, standby.
Step 3, the preparation of precursor storing solution C: weigh polypeptide 3mg, be dissolved in 1ml ultra-pure water, standby
With.
Step 4, the preparation of precursor storing solution D: weigh tellurium powder 1.2g, sodium borohydride 0.08g, be dissolved in
In 1ml ultra-pure water, stand 12 hours, standby.
The synthesis of step 5, polypeptide-quantum dot compound: take 1ml precursor storing solution A, adds 0.05ml
Precursor storing solution B, add the precursor storing solution C of 0.05ml, be eventually adding the precursor deposit of 0.05ml
Liquid D, in being placed on heated at constant temperature metal bath (constant temperature heating device), arranges synthesis condition and is 100 DEG C and adds
Hot 15h, then cools to room temperature, i.e. obtains the polypeptide required for the present invention-quantum dot complex solution.Its
Transmitting wavelength is near 560nm, Fig. 1 and Fig. 2 be shown in by its fluorescence spectrum and photo.
Embodiment 4:
Step one, the preparation of precursor storing solution A: weigh caddy 0.4g, thioglycerol 1.2g, be dissolved in
In 100ml ultra-pure water, regulation solution ph is to 10, standby.
Step 2, the preparation of precursor storing solution B: weigh zinc sulfate 0.03g, thioglycerol 0.05g, dissolve
In 100ml ultra-pure water, regulation solution ph is to 10, standby.
Step 3, the preparation of precursor storing solution C: weigh polypeptide 5mg, be dissolved in 1ml ultra-pure water, standby
With.
Step 4, the preparation of precursor storing solution D: weigh tellurium powder 0.04g, sodium borohydride 0.025g, dissolve
In 1ml ultra-pure water, stand 12 hours, standby.
The synthesis of step 5, polypeptide-quantum dot compound: take 1ml precursor storing solution A, adds 0.02ml
Precursor storing solution B, add the precursor storing solution C of 0.05ml, be eventually adding the precursor deposit of 0.03ml
Liquid D, in being placed on heated at constant temperature metal bath (constant temperature heating device), arranges synthesis condition and is 100 DEG C and adds
Hot 20h, then cools to room temperature, i.e. obtains the polypeptide required for the present invention-quantum dot complex solution.Its
Transmitting wavelength is near 570nm, Fig. 1 and Fig. 2 be shown in by its fluorescence spectrum and photo.
Embodiment 5:
Step one, the preparation of precursor storing solution A: weigh caddy 0.3g, thioglycerol 1.2g, be dissolved in
In 100ml ultra-pure water, regulation solution ph is to 10, standby.
Step 2, the preparation of precursor storing solution B: weigh zinc sulfate 0.03g, thioglycerol 0.05g, dissolve
In 10ml ultra-pure water, regulation solution ph is to 10, standby.
Step 3, the preparation of precursor storing solution C: weigh polypeptide 1mg, be dissolved in 1ml ultra-pure water, standby
With.
Step 4, the preparation of precursor storing solution D: weigh tellurium powder 0.040g, sodium borohydride 0.025g, dissolve
In 1ml ultra-pure water, stand 12 hours, standby.
The synthesis of step 5, polypeptide-quantum dot compound: take 1ml precursor storing solution A, adds 0.05ml
Precursor storing solution B, add the precursor storing solution C of 0.03ml, be eventually adding the precursor deposit of 0.04ml
Liquid D, is placed in heated at constant temperature metal bath (constant temperature heating device), and arranging synthesis condition is 95 DEG C of heating
36h, then cools to room temperature, i.e. obtains the polypeptide required for the present invention-quantum dot complex solution.Its
Ejected wave length is near 590nm, Fig. 1 and Fig. 2 be shown in by its fluorescence spectrum and photo.
Embodiment 6:
Step one, the preparation of precursor storing solution A: weigh caddy 0.4g, thioglycerol 1.2g, be dissolved in
In 100ml ultra-pure water, regulation solution ph is to 10, standby.
Step 2, the preparation of precursor storing solution B: weigh zinc sulfate 0.06g, thioglycerol 0.1g, dissolve
In 10ml ultra-pure water, regulation solution ph is to 10, standby.
Step 3, the preparation of precursor storing solution C: weigh polypeptide 1mg, be dissolved in 1ml ultra-pure water, standby
With.
Step 4, the preparation of precursor storing solution D: weigh tellurium powder 0.040g, sodium borohydride 0.08g, dissolve
In 1ml ultra-pure water, stand 12 hours, standby.
The synthesis of step 5, polypeptide-quantum dot compound: take 1ml precursor storing solution A, adds 0.1ml's
Precursor storing solution B, adds the precursor storing solution C of 0.05ml, is eventually adding the precursor storing solution of 0.05ml
D, is placed in heated at constant temperature metal bath (constant temperature heating device), and arranging synthesis condition is 90 DEG C of heating 48h,
Then cool to room temperature, i.e. obtain the polypeptide required for the present invention-quantum dot complex solution.It launches wavelength
Near 630nm, Fig. 1 and Fig. 2 be shown in by its fluorescence spectrum and photo, its with cell incubation after microscopic fluorescence
Imaging results is shown in Fig. 3, it can be seen that the fluorescence signal of quantum dot in the fluorescence signal of cell membrane, explanation amount
Son point enters cell.
Owing to synchronizing to have carried out synthesis and modified in quantum dot building-up process, synthesis in water quantum can be avoided
Point modification again produces the problems such as quantum dot fluorescence reduction, uses constant-temperature metal bath heating, the side of having
Method is easy, reaction condition is controlled, the time is short, high repeatability and other advantages, and product is made up of multiple element, only
Need to change the ratio of element and the reaction time just can synthesize the quantum dot of different fluorescence.Molecular biology,
The aspects such as cell biology, medical diagnostics and biomedical living imaging have great application prospect.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all at this
Any amendment, equivalent and the improvement etc. made within bright spirit and principle, should be included in the present invention
Protection domain within.
Claims (2)
1. the synthetic method of one peptide species-quantum dot nano complex solution, it is characterised in that described synthesis
Method comprises the steps:
Step one, weigh caddy, thioglycerol and caddy, thioglycerol be dissolved in ultra-pure water,
Regulation solution ph 9-10, obtains the first precursor storing solution；
Step 2, weigh zinc sulfate, thioglycerol and zinc sulfate, thioglycerol be dissolved in ultra-pure water,
Regulation solution ph 9-10, obtains the second precursor storing solution；
Step 3, weigh polypeptide and polypeptide is dissolved in ultra-pure water, obtaining the 3rd precursor storing solution；
Step 4, weigh tellurium powder, sodium borohydride and tellurium powder, sodium borohydride be dissolved in ultra-pure water, standing,
Obtain the 4th precursor storing solution；
Step 5, take the first precursor storing solution, add the second precursor storing solution of different volumes, add not
3rd precursor storing solution of same volume, is eventually adding the 4th precursor storing solution of different volumes, is mixed by final
Close liquid heating and be then cooled to room temperature, i.e. obtain polypeptide-quantum dot nano complex solution；
In step 4, time of repose is 12 hours；In step 5, heating condition is: in heated at constant temperature
90-100 DEG C of heating in device, the heat time is 1 hour to 48 hours.
2. polypeptide-quantum dot nano compound that prepared by a synthetic method according to claim 1 is molten
Liquid.
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|---|---|---|---|---|
| WO2011060353A2 (en) * | 2009-11-16 | 2011-05-19 | Emory University | Lattice-mismatched core-shell quantum dots |
| CN102311736A (en) * | 2011-07-06 | 2012-01-11 | 天津工业大学 | Preparation method of CdTe/CdS/SiO2 composite fluorescent nanoparticle |
| CN102559190A (en) * | 2012-01-12 | 2012-07-11 | 东南大学 | Dual-mode optical coding probe and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2011060353A2 (en) * | 2009-11-16 | 2011-05-19 | Emory University | Lattice-mismatched core-shell quantum dots |
| CN102311736A (en) * | 2011-07-06 | 2012-01-11 | 天津工业大学 | Preparation method of CdTe/CdS/SiO2 composite fluorescent nanoparticle |
| CN102559190A (en) * | 2012-01-12 | 2012-07-11 | 东南大学 | Dual-mode optical coding probe and preparation method thereof |
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