CN103897211A - Elastic cerebral dura mater - Google Patents
Elastic cerebral dura mater Download PDFInfo
- Publication number
- CN103897211A CN103897211A CN201210589564.4A CN201210589564A CN103897211A CN 103897211 A CN103897211 A CN 103897211A CN 201210589564 A CN201210589564 A CN 201210589564A CN 103897211 A CN103897211 A CN 103897211A
- Authority
- CN
- China
- Prior art keywords
- collagen
- dura mater
- cerebral dura
- dimensional structure
- type
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/34—Materials or treatment for tissue regeneration for soft tissue reconstruction
Abstract
The invention discloses a scaffold material for the clinical reconstruction of a cerebral dura mater and a preparation method of the scaffold material, belongs to the field of the medical biomaterials, and mainly aims at solving the problems of limited drawing and complex operation of an autologous membrane, and difficulty in obtaining, high price and high risk of a xenogenous cerebral dura mater, and not easy preservation and easy sticking of biological substituent materials in clinic at present. The purpose of repairing the cerebral dura mater is achieved by providing a cerebral dura mater receptor with an active substrate for cerebral dura mater cell growth and a certain spatial three-dimensional structure and by preventing adhesion of the material to a receptor cranium by use of single-sided treatment. The material prepared by use of the method has a three-dimensional structure that a type I collagen inherently has different concentrations, and therefore, the material is good in compactness and even, has certain elasticity and tenacity, and further is pressure-resistant and capable of preventing the leakage of the cerebrospinal fluid; besides, the original activity of the collagen is maintained, and thus advantageous for the growth of the cerebral dura mater cells; in addition, the probability of post-operation adhesion of the material to the cranium is reduced by use of single-sided physical cross-linking; and the material is good in biocompatibility and thus advantageous for the repair and reconstruction of the cerebral dura mater.
Description
Technical field
The present invention relates to the biomembrane material that a kind of type i collagen makes through physics, chemical process processing.Have certain elasticity and toughness, stopping property and biocompatibility are better.For the prosthetic dural substitutes of brain surgery endocranium.Belong to biomaterial for medical purpose field.
Background technology
In world's brain surgery operation of opening cranium at present, in order to prevent that cerebrospinal fluid seepage from causing intracranial infection, the patient that need to do artificial dura mater reparation has 10%～15%.Be mainly reflected in: cerebral trauma coup injury, the infiltration of tumour, endocranium open decompression in surgical procedure, and the dura defect that causes of some geneogenous factors.The dural repairment material that used is used is autologous film, but due to the restriction of the size and dimension of being drawn materials with perform the operation numerous and diverse and produce the factors such as new wound, the autologous film of damaged employing that is mostly small area substitutes endocranium, large-area damaged or will lean on other dural substitutes to complete.
Vehicles Collected from Market dural substitutes has Homologous dura, biological substitution material endocranium.Homologous dura has two significant weakness, and the one, material source is limited and expensive; The 2nd, the most fatal weakness, the existence of prion: human body dry freeze endocranium may start an inflammation of the liver, the infection of immunodeficiency virus or bacterium etc.Biological substitution material is very effective as dural substitutes, has good biological fitness, and easy handling, wide material sources, cheap, is a kind of dural substitutes that has development potentiality.Domestic bovine pericardium, sheep pericardium, Pigs Hearts bag, ox peritonaeum, pig peritonaeum, the mesentery etc. of often using clinically replace endocranium.But there is again the adhesion of causing simultaneously, be difficult for preserving, be difficult for sterilization, the shortcomings such as immune response likely occur.
In view of this, develop a kind of good biocompatibility; There are certain elasticity and toughness; Can after generating, cambium be completely absorbed; Anti; Easily preserve, easy to operate; Material source is extensive; It is particularly important that the biological endocranium that price is relatively moderate becomes.Because natural dural main component is fiber collagen, therefore having adopted, our company utilizes own intrinsic three-dimensional structure under type i collagen different concns, by chemistry, the method that the method for physics is assembled the polymerization collagem membrane that forms metastable spatial configuration of molecules makes, the dural substitutes space structure compactness that this method makes is good, evenly, there are certain elasticity and toughness, resistance to compression, can effectively prevent cerebrospinal fluid seepage, and maintain original activity of collagen, be beneficial to the growth of endocranium cell, moreover reduce and the adhesion probability of cranium by the method for one side physical crosslinking, for endocranium reparation, the powerful guarantee that provides of epilepsy is provided.
Summary of the invention
This material selection type i collagen assembled by the method for chemistry, physics the method that forms metastable spatial configuration of molecules and made.Original activity that can maintain collagen reaches the requirement that endocranium is repaired.Main operational steps is as follows:
1, the first of type i collagen three-dimensional structure built: commercially available type i collagen, be soaked in the hydrochloric acid soln of 0.01mol/L～1.0mol/L, and make collagen solution or collagen gel that concentration is 0.1%～60.0% (w/w).
2, the processing of the fixing and anti of type i collagen three-dimensional structure: the type i collagen of the good structure of Primary Construction is carried out to plastic film mulch according to required size, then the one side of film is exposed under ultraviolet lamp, carry out UV-crosslinked, crosslinking time 30min～500min.
3, the gathering of type i collagen: by commercially available sodium hydroxide, be mixed with the solution that concentration is 0.001mol/L～2.0mol/L, sterilising treatment, is then immersed in 1min～30min in sodium hydroxide solution by the collagem membrane of and Anti-adhesive fixing through three-dimensional structure.Final pH value is (6～8).
4, the dural formation of elasticity: by the collagem membrane dehydration of having assembled, making its water content is 5.0%～40.0%, packs irradiation sterilization.
Embodiment
Embodiment 1,
Get commercially available type i collagen 1.0g, being immersed in 100ml concentration is in 0.2mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 1.0mol/L sodium hydroxide solution and soak 2min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 40.0%.Pack rear irradiation sterilization.
Embodiment 2,
Get commercially available type i collagen 10.0g, being immersed in 100ml concentration is in 0.05mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 300min in UV-crosslinked instrument.Then put in 0.001mol/L sodium hydroxide solution and soak 15min, pH value is 7.By the dehydration of gained polymerization collagen, making its water content is 10.0%.Pack rear irradiation sterilization.
Embodiment 3,
Get commercially available type i collagen 15.0g, being immersed in 100ml concentration is in 0.5mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 2.0mol/L sodium hydroxide solution and soak 1min, pH value is 6.By the dehydration of gained polymerization collagen, making its water content is 20.0%.Pack rear irradiation sterilization.
Embodiment 4,
Get commercially available type i collagen 60.0g, being immersed in 100ml concentration is in 1.0mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 1.0mol/L sodium hydroxide solution and soak 2min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 10.0%.Pack rear irradiation sterilization.
Embodiment 5,
Get commercially available type i collagen 0.1g, being immersed in 100ml concentration is in 0.01mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 90min in UV-crosslinked instrument.Then put in 0.001mol/L sodium hydroxide solution and soak 2min, pH value is 6.By the dehydration of gained polymerization collagen, making its water content is 30.0%.Pack rear irradiation sterilization.
Embodiment 6,
Get commercially available type i collagen 30.0g, being immersed in 100ml concentration is in 0.2mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 150min in UV-crosslinked instrument.Then put in 0.05mol/L sodium hydroxide solution and soak 2min, pH value is 6.By the dehydration of gained polymerization collagen, making its water content is 20.0%.Pack rear irradiation sterilization.
Embodiment 7,
Get commercially available type i collagen 20.0g, being immersed in 100ml concentration is in 0.1mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 20min in UV-crosslinked instrument.Then put in 1.0mol/L sodium hydroxide solution and soak 2min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 40.0%.Pack rear irradiation sterilization.
Embodiment 8,
Get commercially available type i collagen 0.8g, being immersed in 100ml concentration is in 0.7mol/L hydrochloric acid soln.Fixing shaping after fully soaking, crosslinked 200min in UV-crosslinked instrument.Then put in 0.05mol/L sodium hydroxide solution and soak 20min, pH value is 8.By the dehydration of gained polymerization collagen, making its water content is 5.0%.Pack rear irradiation sterilization.
Claims (1)
1. assembled by the method for chemistry, physics the polymerization collagem membrane that forms metastable spatial configuration of molecules by the intrinsic three-dimensional structure of type i collagen itself for one kind, its compactness is good, evenly, there are certain elasticity and toughness, resistance to compression, can effectively prevent cerebrospinal fluid seepage, and maintain original activity of collagen, be beneficial to the growth of endocranium cell, moreover reduce and the adhesion probability of cranium by the method for one side physical crosslinking, for endocranium reparation, prevent the powerful guarantee that provides of epilepsy.Its feature comprises the following steps
(1), commercially available type i collagen, be soaked in the hydrochloric acid soln of 0.01mol/L～1.0mol/L, make collagen solution or collagen gel that concentration is 0.1%～60.0% (w/w).Just build the three-dimensional structure of certain space.
(2), the type i collagen of the good structure of Primary Construction is carried out to plastic film mulch according to required size, then the one side of film is exposed under ultraviolet lamp, carry out UV-crosslinked, crosslinking time 30～500min.The three-dimensional structure of certain space is fixed and ultraviolet processing, with anti.
(3), by commercially available sodium hydroxide, be mixed with the solution that concentration is 0.001mol/L～2.0mol/L, sterilising treatment, is then immersed in 1～30min in sodium hydroxide solution by the collagem membrane of and Anti-adhesive fixing through three-dimensional structure.Final pH value is (6～8).Assemble the three-D space structure of stablizing type i collagen.
(4), by the collagem membrane dehydration of having assembled, making its water content is 5.0%～40.0%, packs irradiation sterilization.Make elasticity endocranium.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210589564.4A CN103897211A (en) | 2012-12-26 | 2012-12-26 | Elastic cerebral dura mater |
| PCT/CN2013/000733 WO2014101254A1 (en) | 2012-12-26 | 2013-06-24 | Elastic dura mater |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210589564.4A CN103897211A (en) | 2012-12-26 | 2012-12-26 | Elastic cerebral dura mater |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103897211A true CN103897211A (en) | 2014-07-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210589564.4A Pending CN103897211A (en) | 2012-12-26 | 2012-12-26 | Elastic cerebral dura mater |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN103897211A (en) |
| WO (1) | WO2014101254A1 (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1463984A (en) * | 2002-06-10 | 2003-12-31 | 于海鹰 | Medicinal collagen material and its making process |
| CN103071189A (en) * | 2013-01-25 | 2013-05-01 | 广州华美康联生物科技有限公司 | Preparation method of collagen film for guided tissue regeneration |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9721585D0 (en) * | 1997-10-10 | 1997-12-10 | Geistlich Soehne Ag | Chemical product |
| JPH09122227A (en) * | 1995-10-31 | 1997-05-13 | Bio Eng Lab:Kk | Medical material and manufacture thereof |
| WO2006029571A1 (en) * | 2004-09-14 | 2006-03-23 | The University Of Hong Kong | Photochemically crosslinked collagen scaffolds and methods for their preparation |
| CN102716517B (en) * | 2011-03-30 | 2015-01-14 | 深圳兰度生物材料有限公司 | Guided tissue regeneration membrane and its preparation method |
-
2012
- 2012-12-26 CN CN201210589564.4A patent/CN103897211A/en active Pending
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2013
- 2013-06-24 WO PCT/CN2013/000733 patent/WO2014101254A1/en active Application Filing
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1463984A (en) * | 2002-06-10 | 2003-12-31 | 于海鹰 | Medicinal collagen material and its making process |
| CN103071189A (en) * | 2013-01-25 | 2013-05-01 | 广州华美康联生物科技有限公司 | Preparation method of collagen film for guided tissue regeneration |
Non-Patent Citations (1)
| Title |
|---|
| JONG-EUN LEE，ET AL.: "Characterization of UV-irradiated Dense/porous Collagen Membranes：Morphology，Enzymatic Degradation，and Mechanical Properties", 《YONSEI MEDICAL JOURNAL》, vol. 42, no. 2, 31 December 2001 (2001-12-31), pages 172 - 179 * |
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| Publication number | Publication date |
|---|---|
| WO2014101254A1 (en) | 2014-07-03 |
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