CN1038949A - The aflatoxin-contaminated cereal or the density fractionation of nut - Google Patents

The aflatoxin-contaminated cereal or the density fractionation of nut Download PDF

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Publication number
CN1038949A
CN1038949A CN 88103997 CN88103997A CN1038949A CN 1038949 A CN1038949 A CN 1038949A CN 88103997 CN88103997 CN 88103997 CN 88103997 A CN88103997 A CN 88103997A CN 1038949 A CN1038949 A CN 1038949A
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described method
aflatoxin
density
peanut
nut
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CN 88103997
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CN1025418C (en
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詹姆斯·查尔斯·亨德森
斯坦利·亨利·克罗伊策
阿瑟·艾尔弗雷德·施米特
查尔斯·艾尔弗雷德·史密斯
威廉·罗德尼·哈根
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Procter and Gamble Co
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Procter and Gamble Co
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Priority to CN 88103997 priority Critical patent/CN1025418C/en
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Publication of CN1025418C publication Critical patent/CN1025418C/en
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  • Apparatuses For Bulk Treatment Of Fruits And Vegetables And Apparatuses For Preparing Feeds (AREA)
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Abstract

Disclosed is a kind of will being separated with unpolluted whole grain cereal seed, have benevolence nut and seed whole or that split by the cereal of mycotoxin contamination, kernel, seed and nut, to obtain a kind of source of supply that comes down to unpolluted these foods.Contaminated that part of can further processing of separating to reduce the mycotoxin contamination degree.The method is included in the food that floating pollution in a kind of liquid has aflatoxin, thereby the material that will contain mycotoxin or aflatoxin is separated with densimetry.A kind of very preferable methods is to use the power flotation.

Description

The aflatoxin-contaminated cereal or the density fractionation of nut
The present invention relates to a kind of with unpolluted whole grain seed-grain, whole or broken kernel, nut and seed with separately, with the method for the unpolluted source of supply basically that obtains these food by the cereal of mycotoxin contamination, kernel, seed and nut.This method comprises and will be swum in a kind of liquid by the food of mycotoxin contamination, utilizes the density difference to isolate the material that contains mycotoxin.Preferably can provide the foodstuff of mycotoxin content within level of security with the further processing of the contaminated part of separating.
Mycotoxin is a big class noxious material of growing on food and producing owing to fungi.Aflatoxin be owing to a kind of fungi that belongs to aspergillus at cereal, seed, kernel or effect produces above the nut a kind of special mycotoxin.Aflatoxin contamination is the problem of a particular importance in peanut industry.In addition, aflatoxin also can influence cereal such as corn, hops, also can influence oilseeds (as cotton seed), soybean, almond, Brazil nut, hickory nut, pistachio fruit etc.The fungi growth that the good condition of nut and corn growing is equally also helped producing aflatoxin.
Since verified in animal experiment the aflatoxin content height can cause carcinogenesis, therefore, aflatoxin content is high in the food can threaten the animal and the mankind.For this reason, United States Department of Agriculture has stipulated the highest limiting the quantity of to the aflatoxin in the food and other mycotoxins.
The someone attempts aflatoxin-contaminated food is carried out detoxification, and still, in general effect is very limited, and often produces bad side effect, or local flavor degenerates, or food itself is damaged.So the method that is generally used for handling aflatoxin-contaminated material is to attempt aflatoxin-contaminated material is separated with unpolluted material.Aflatoxin contamination occurs on simple grain grain, kernel, nut or the peanut.So, use the fluorescence detector sorting usually to remove the material that pollutes and to reject the nut that damages by hand.But this method is to detecting aflatoxin-contaminated whole grain nut poor effect, do not damage because a bit see from outward appearance.
United States Patent (USP) 4,035,518 have set forth a kind of colour-selection technique, and the cereal, corn and the nut that in the method pollution are had aflatoxin be with a kind of aqueous solution short-time contact under high temperature, this aqueous solution contain a kind of water miscible, can be with the aflatoxin reagent of food coexistence.Water cleans nut then.It significantly, almost is visible immediately variation that this aflatoxin reagent makes pollution have the nut of aflatoxin to produce a kind of.Then nut is carried out the look choosing.Aflatoxin reagent is alkaline matter such as potassium hydroxide, NaOH normally.
Usually, the aflatoxin contamination amount of corn, grain, seed or nut products is that the aflatoxin that contains parts per billion or a few millionths in the sample with this product is represented.If the amount of representing with parts per billion is lower than the permission content of using as ultimate food, then this sample or this batch materials can be processed.But if sampling the time is missed the higher material of some contaminant capacities, even then the amount of the aflatoxin that contains in the sample is lower, some final finished also may contain very high aflatoxin contamination amount.In addition, it is good a collection of cereal, nut or seed basically that the difference of sampling also can make, and is declared as and can not eats owing to testing out higher content.So a kind of nut or cereal, particularly outward appearance that can separate pollution be it seems and the nut of unspoiled pollution or the method for cereal, will will be very useful.This method can be with contaminated whole grain grain, nut or seed with other whole granule products that do not damage separately, thereby reduce the mycotoxin in the material by the gross or the content of aflatoxin.
Surprisingly, have been found that to pollute have seed, grain or the nut of aflatoxin to have lower density than untainted seed, grain or nut.Therefore, can adopt a kind of liquid floatation method to separate the nut that contains aflatoxin.A kind of method very preferably is the power floatation, this separation method is to finish under the condition of careful control, make no matter be the inside of contaminated or unpolluted cereal, kernel, seed or nut, the infiltration of any degree can not arranged by the density separation medium.This method can be used to separate the whole grain kernel of single pollution, and may leak choosing with additive method.
Floatation has been used in the past unedible field trashes such as the fruit stone, stone, stalk stem in separating oil, seed, shell and the fruit.It also once was used to separating oil from unclassified stores.But, do not find as yet to take from wholesome product, to separate seed, kernel, nut or the cereal that pollution has aflatoxin with floatation.
Therefore, an object of the present invention is to come separately by the nut of mycotoxin contamination, kernel, seed, cereal and unpolluted nut, kernel, seed, cereal with a kind of fluid density separation method.
Another object of the present invention be will it seems intact but the nut, seed, kernel or the grain that are actually pollution with other unpolluted products separately, therefore reduced total mycotoxin content of product.
These purposes and other purposes are illustrated in detailed description of the present invention.
This method introduction be with the liquid floatation method will be placed in the water by the food of mycotoxin contamination or proportion between about 0.9 to 1.2 can other liquid with food coexistence in, and make thus pollute the life that aflatoxin is arranged nut, seed, kernel or cereal with unpolluted material separately.
Fig. 1 is the flow chart of this method.
Fig. 2 and 3 expressions are used for the preferred equipment of products of separated.Fig. 2 is a kind of top view of dynamic separator.In Fig. 2, (1) expression is used for a groove of separating floating material; (3) be the kerve outlet of the nut, seed, kernel or the grain that are used for separate and subside; (5) be for nut and the mobile passage of water; (7) be the device that is used for the pre-wetted nut; (9) be the adjustable plate washer that is used for separating floating thing and precipitum; (11) be the floating thing tapping equipment; (13) be a stoner and (or) recirculating water system of gleanings.Fig. 3 is the side view of same equipment, and expresses dodge gate and separator.
As previously mentioned, the fungi of generation aflatoxin is to grow under the identical general condition of cereal and nut growth.These fungies also can breed under some storage requirement.Therefore normal pollution of cereal has aflatoxin, particularly corn, oat, also has nut, particularly hickory nut, almond, walnut and seed or kernel (as shelled peanut).Method of the present invention is applicable to that after this all be referred to as kernel, seed, cereal and the nut of " foodstuff that pollution has aflatoxin ".This method is specially adapted to peanut, and will describe with the example that is separated into of peanut, but should not think and only be applicable to peanut.
Pollution has the foodstuff of aflatoxin to make whole granule product stay that sieve is gone up and with particle or damage material separately through screening.Suggestion is earlier through just branch or screening step are removed all stones, bulky grain chip, shell, withe etc.
Usually, the mesh screen size that is used for peanut should be able to be sieved and be stayed whole grain peanut.The unspoiled peanut of all whole grains is no matter its size how, can add together with the shelled peanut of staying on the mesh screen.This primary dcreening operation can be removed all peanut products broken or that damage less than prescribed level.
Usually, the water content of peanut is between 6% to 10.5%.Here preferably use the peanut of water content between about 6% to 9%.
Floatation equipment includes container and sieve, groove or other pollution that can be used for separating floating of putting flotation medium the device without the kernel, nut, seed, cereal or the peanut that pollute of the foodstuff of aflatoxin with sedimentation.A kind of simple floatation equipment is the bucket or the cylinder that fill flotation medium, with screen cloth floating product is told in this container.
A kind of preferred floatation equipment is shown in Fig. 2 and 3.This is George J.Olney, Inc., Westernville, the remodeling of the pea separator that New York produces.The machine of the same type of other food processing equipment distributors also can be used for the method.
The foodstuff that pollution has an aflatoxin gets off from vibratory sieve and enters into separation equipment through the hopper in (1) top.Water flows into (1), (3) and (13) part, and with the foodstuff skewed slot (5) of flowing through together.Floating thing flows out on the mesh screen of a vibration.The peanut that sinks is fallen on second vibrating screen.
Flotation medium can be the material of any density between 0.9 to 1.2 scope, but, so must be edible and/or can share with food because this flotation medium will be with contacting for animal and human class nut, kernel, cereal or the seed as food.Preferred flotation medium is a water.Outside dewatering, also can use salting liquid, salt solution, sugar juice, glycerine, alcohol or alcohol water blend and edible wet goods.Can add antioxidant, antifungal agent and antibacterial agent in the flotation medium.
Mixture, soya-bean oil, corn oil, peanut oil and the sunflower oil of the sodium chloride solution that preferred flotation medium is water, 0.001-1.0M, ethanol, second alcohol and water.These oil can carry out partial hydrogenation.
Preferably earlier peanut or other pollutions there are the foodstuff pre-wetted of aflatoxin or carry out moistening in the phase I of lock out operation.The pre-wetted on peanut surface can be destroyed the surface tension of flotation medium, thereby makes the peanut sedimentation.Pre-wetted can be when product enters separator or before entering be finished by spray, also product can be placed under the flotation medium liquid level access to plant and finish.The mode of pre-wetted should make the time of material contact medium minimize.This can prevent owing to material is exposed to the local flavor degraded that makes material in higher moisture or other materials such as oil and alcohol.Can prevent that also sealing or other flotation mediums infiltrate through the inside of product.
In general, therefore the density of untainted seed, kernel, nut or grain sink greater than 1.For example, the density of whole grain peanut on average is about 1.05 to 1.2.Pollution has the density of peanut of aflatoxin less than 1, usually between 0.98 to 0.99, and therefore come-up.
In a preferred embodiment, peanut or other pollutions have the foodstuff of aflatoxin to enter separator by an oscillating feeder.They are the slit by a weak point or skewed slot and stone is branched away by a little open slot then.Usually should have a slight hydraulic pressure to make progress from slit, nut or grain that common meeting is sunk in flotation medium remain in the flotation medium not sink, and have only the heaviest material, and promptly stone could fall by slit.This pressure can water mobilization dynamic produce, be generally less than per minute pottle flow velocity.Also can use air or any other pneumatic or hydraulic means.
Peanut (and unclassified stores) enters the speed of separator from about 4000 to 10,000 Pounds Per Hours.
Peanut is by groove and fall in the water.If this nut was once crossed pre-wetted, then do not need extra dipping.Make cereal or nut pass through flotation medium.The temperature of flotation medium is unimportant.Usually, 45 to 80 temperature gets final product.Floating thing is discharged from the top, and precipitum is then discharged in the bottom of separator.
Most preferred method includes power flotation effect.In the method, pollute the foodstuff that aflatoxin is arranged and in the medium contact, stirred.This can realize by using vibratory sieve, hydraulic pressure, water turbulent flow or air pressure.Can use the flow velocity of per minute about 250 to about 350 gallons waters or liquid.Such flow velocity combines with above-mentioned charging rate, can obtain good centrifugation.
Then floating thing and precipitum are placed on the vibratory sieve removing all flotation mediums, the very easy venting of these media from the teeth outwards if desired, is also capable of circulationly used again.Can be with air circulation by the eliminating of sieve with the acceleration flotation medium.
Nut or grain total time generally were less than for 30 seconds with the flotation medium contact, but also can be how by 5 minutes.The length of time of contact depends on that the medium porous goes into foodstuff and change its density and influence time of its local flavor or quality.In addition, flotation medium infiltrates through the degraded that peanut, nut or grain can cause local flavor for a long time.Particularly water more can produce this problem.Time with the medium contact is less than 5 minutes usually.Preferably time of contact from about 5 seconds are to 180 seconds, particularly from about 5 seconds to 30 seconds.
Sedimentation products flows through a drying device immediately.Also can use any device that can evaporate flotation medium.Can applied microwave, heating means such as radiation or convection current.Product drying is arrived its original water content.Will be concerning peanut less than 10.5%, water content is between 6% to 9% usually.Importantly should be with product drying to its original water content, because this shows that the flotation medium that is stained with on the surface is all removed.As be dried to and be lower than original water content, illustrating that the moisture content of nut or seed inside also has been removed, this will change this product.
Temperature in the drier should keep low temperature, to guarantee not damage or bake nut or cereal.Concerning most of seeds, kernel, nut and cereal, about 125 to 250 temperature range is suitable.After the drying, material can make its cooling by a cooling device.
As containing salt or sugar in the flotation medium, or other material beyond dewatering, then should be earlier that it is clean before dry.
Almost all the whole grain peanut of sedimentations does not contain aflatoxin or only contains the aflatoxin of denier.Can carry out drying this moment to it.
Dried peanut can pass through a color selector or other aflatoxin detector.Nut any dark color or that pollute all can be rejected through this system the time.Then the peanut of light color, nut, cereal, kernel or seed are processed into food, oil or other esculent.
Have the food of aflatoxin darker than untainted product colour usually because pollute, therefore available color selector is rejected it.Product joins on the vibration entry table from hopper, and entry table moves on to product on two counter-rotational rollers.It is single-row that these rollers are lined up product, and every like this granule product can be observed through probe separately.When particle process probe, every granule product is observed by three photocells.These photocells detect the reverberation (brightness) of each particle, and same this base plate of contrast compares.As the shade that the shortcoming part is arranged on the fruit granule reverberation is had not simultaneously with the contrast of this base plate, this particle can be disallowable.
Probe is backlighted.Light is reflected on the photomultiplier optical element with this base plate from particle.
The selection of this base plate color and luster should make its reverberation with the reverberation of most of particle surfaces same intensity be arranged.Like this, no matter be the color and luster homogeneous granules through eyelet, perhaps be empty before the eyelet, reflex to only identical on the photomultiplier.
The floating nut that contains the higher amount aflatoxin can be by blanching after super-dry, and then carries out the look choosing.
These nuts use conventional blanching method to carry out blanching.Usually, belt or roller are arranged in the blanching device, these belts or roller are removed epidermis from nut or cereal, as are nut, also can be with its division in flakes.Concerning peanut, then in two.With a visible light aflatoxin detector these nuts that split through the branch of blanching are carried out the look choosing again.With untainted nut dark color, that pollute the same light color of nut that aflatoxin is arranged separately.Those nuts by the look choosing can be used for food processing usually.That part ofly can be used as other purposes through what the look despecking removed, perhaps whether can further process and make decision it.
Usually, preferred power density separation method can be isolated about 80% to 95% precipitum peanut or untainted product, and about floating thing of 5% to 20%.The amount of separating is decided with the degree of the original pollution of product.The aflatoxin content of floating thing is more much bigger than the content in primary sample and the precipitum.
Example 1
45,400 pounds of shelled peanuts that will contain 0.6% grating peanut sieve.Initial moisture content is 7.5%.1.4% shelled peanut in screening is because big or small, damage or broken and disallowable.Those stay 15/64 inch part on the mesh screen through a flotation unit then, and this device is the modified separator of Olney company.Feeder is the vibratory sieve of 66 pounds to 166 pounds of per minute operations.
Peanut swum in the water (per hour has 4000 to 10000 pounds of shelled peanuts in water, 300 to 350 gallons of the flow velocity per minutes of water, about 60 of water temperature).Under a plate washer, pass to carry out moistening when peanut enters separator.90% shelled peanut sedimentation is arranged approximately, and about 10% shelled peanut is floating.They were approximately for 30 seconds by the time of separator.Per hour about 5000 pounds of throughput.The final water content of floating shelled peanut about 13 ± 1%.
Then shelled peanut is placed on the vibratory sieve by a Roasting oven that contains three thermals treatment zone.The temperature in three districts is respectively 180 °F, 130 °F and 130 °F.Then with shelled peanut by a cooler to be cooled to about 80 room temperature.Usually, shelled peanut is about 45 minutes through the time of Roasting oven.What this system adjusted to shelled peanut contact high-moisture is less than about three minutes total time.Be to make the water content of product reduce to original level of recovering remaining drying time.
Be equipped with the look of visible light detector to select device with the peanut drying of sedimentation and by one then.The color of nearly 99.5% shelled peanut is qualified.
Aflatoxin total content in the original shelled peanut is on average less than 10ppb(10/1000000000ths).The about 8ppb of aflatoxin average content of qualified shelled peanut.
The shelled peanut that the subsidence part lease making is crossed the screening rejecting is about 0.5%.The aflatoxin content of rejecting whole groundnut kernels in the part is about 22ppb.
Floating shelled peanut separates after super-dry and blanching.Aflatoxin content is about 49ppb.Shelled peanut with blanching carries out the look choosing then.It is qualified that 91% color is arranged in these shelled peanuts approximately.The about 14ppb of aflatoxin content of the qualified shelled peanut of these colors.These qualified shelled peanuts are added to recycle in the charging peanut stream again and carry out FLOTATION SEPARATION.The shelled peanut of rejecting is 8.7% of an original floating part, or is the about 2% of original shelled peanut, and the aflatoxin content of the shelled peanut of rejecting is about 129ppb.
This method can be isolated the extremely low part (less than 10ppb) of about 98% aflatoxin content and the 2% highly polluted part that aflatoxin arranged (content from more than the 10ppb to 129ppb).
Example 2
Handle the shelled peanut that second batch of aflatoxin content is about 4ppb with the same method in the example 1.This a collection of shelled peanut has 98.1% sedimentation and 1.9% floating.Through look choosing and screening again, the good shelled peanut of whole grain of sedimentation has 98.8% after the drying, and its aflatoxin content is about 1ppb.The shelled peanut that splits also contains the aflatoxin of the 1ppb that has an appointment.The sedimentation shelled peanut is through the look choosing.The sedimentation shelled peanut is through the look choosing disallowable that part of about 8ppb of aflatoxin that contains in back.
In the floating thing,, there is the qualified peanut of 92.9% blanching to contain the about 5ppb of aflatoxin through the look choosing.The aflatoxin content of the floating peanut of the process blanching of about 7.1% rejecting is about 79ppb.

Claims (24)

1, a kind of foodstuff that will not contain aflatoxin in fact is with the method for polluting the foodstuff separation that aflatoxin is arranged, and this method comprises:
(a) be about 0.9 to 1.2 flotation medium with polluting the foodstuff contact that aflatoxin is arranged with a kind of density, and this foodstuff is divided into two parts, the density of a part is less than density of medium, and the density of another part is equal to or greater than density of medium;
(b) dry this two parts nut or cereal are to their original water content.
2, the described method of a kind of claim 1, wherein density is first by blanching less than the part of flotation medium, and is dry then.
3, the described method of a kind of claim 2, wherein each part is all passed through the look choosing.
4, the charging rate that the described method of a kind of claim 3, wherein said pollution have the foodstuff of aflatoxin to be added in the flotation medium is about per hour 4000 pounds to 10,000 pounds.
5, the described method of a kind of claim 4, wherein the density of flotation medium is about 1.0 to 1.05.
6, the described method of a kind of claim 5, wherein said flotation medium are water or rare salting liquid.
7, the described method of a kind of claim 6, wherein said edible material is a peanut.
8, the described method of a kind of claim 7, wherein said peanut water pre-wetted is to water content about 13%.
9, the described method of a kind of claim 8, wherein said peanut are dry under about 125 to 250 temperature.
10, the described method of a kind of claim 8, wherein said flotation are finished being less than in 30 seconds.
11, the described method of a kind of claim 3, wherein said flotation medium are selected from salt solution, edible alcohols, glycerose solution and edible oil.
12, the described method of a kind of claim 3, wherein said edible material comprise and are selected from a kind of in one group of material such as corn, oat, almond and walnut.
13, a kind of edible material that will not contain aflatoxin in fact is with the method for polluting the edible feed separation that aflatoxin is arranged, and this method comprises:
(a) be about 0.9 to 1.2 dynamic flotation medium with polluting the edible material contact that aflatoxin is arranged with a kind of density, this material is divided into two parts, the density of a part is less than density of medium, and the density of another part is equal to or greater than density of medium;
(b) dry this two parts nut or cereal are to their original water content.
14, the described method of a kind of claim 13, wherein said flotation medium are water or rare salting liquid.
15, the described method of a kind of claim 14, wherein the flow velocity of flotation medium is 200 gallons to 350 gallons of per minutes.
16, the described method of a kind of claim 15, wherein the density of flotation medium is about 1.0 to 1.05.
17, the described method of a kind of claim 16, wherein density is first by blanching less than the part of flotation medium, and is dry then.
18, the described method of a kind of claim 17, wherein each part is all passed through the look choosing.
19, the described method of a kind of claim 18, it is peanut that wherein said pollution has the edible material of aflatoxin.
20, the described method of a kind of claim 19, wherein said peanut water pre-wetted is to water content about 13%.
21, the described method of a kind of claim 20, wherein said peanut are dry under about 125 to 250 temperature.
22, the described method of a kind of claim 21, wherein said flotation are finished being less than in 30 seconds.
23, the described method of a kind of claim 16, wherein said flotation medium are selected from salt solution, edible alcohols, glycerose solution and edible oil.
24, the described method of a kind of claim 16, wherein said pollution have the edible material of aflatoxin to comprise to be selected from a kind of in one group of material such as corn, oat, almond and walnut.
CN 88103997 1988-06-30 1988-06-30 Density separation of aflatoxin-contaminated grain or nuts Expired - Fee Related CN1025418C (en)

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Application Number Priority Date Filing Date Title
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CN1025418C CN1025418C (en) 1994-07-13

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104703957A (en) * 2012-10-12 2015-06-10 嘉吉公司 Method and apparatus for reducing aflatoxin-contaminated corn
CN106311454A (en) * 2015-07-03 2017-01-11 中国科学院理化技术研究所 Fruit screening method and fruit smashing method
CN109499751A (en) * 2018-12-04 2019-03-22 中国林业科学研究院亚热带林业研究所 A kind of selected processing technology of walnut
CN111418758A (en) * 2020-05-08 2020-07-17 武汉轻工大学 Method for reducing vomitoxin content of wheat, wheat flour and flour product
CN113141809A (en) * 2021-04-13 2021-07-23 湖南省森林植物园 Pecan seed preservation and seedling raising method

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104703957A (en) * 2012-10-12 2015-06-10 嘉吉公司 Method and apparatus for reducing aflatoxin-contaminated corn
CN107080150A (en) * 2012-10-12 2017-08-22 嘉吉公司 For the method and apparatus for the corn for reducing aflatoxin contamination
CN106311454A (en) * 2015-07-03 2017-01-11 中国科学院理化技术研究所 Fruit screening method and fruit smashing method
CN106311454B (en) * 2015-07-03 2019-02-15 中国科学院理化技术研究所 A kind of screening technique and breaking method of fruit
CN109499751A (en) * 2018-12-04 2019-03-22 中国林业科学研究院亚热带林业研究所 A kind of selected processing technology of walnut
CN111418758A (en) * 2020-05-08 2020-07-17 武汉轻工大学 Method for reducing vomitoxin content of wheat, wheat flour and flour product
CN113141809A (en) * 2021-04-13 2021-07-23 湖南省森林植物园 Pecan seed preservation and seedling raising method
CN113141809B (en) * 2021-04-13 2022-06-07 湖南省森林植物园 Pecan seed preservation and seedling raising method

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