CN103893818A - Osteochondral three-dimensional stent with regular interpenetrating network structure and preparation method thereof - Google Patents
Osteochondral three-dimensional stent with regular interpenetrating network structure and preparation method thereof Download PDFInfo
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Abstract
The invention discloses an osteochondral three-dimensional stent with a regular interpenetrating network structure. A biological medical high polymer material system and an in-situ generated hydrogel material system form the regular interpenetrating network structure. The osteochondral three-dimensional stent is provided with a multi-layer structure which is divided into a calcified layer, a radiation layer, a transition layer and a superficial layer. The invention further discloses a preparation method of the osteochondral three-dimensional stent. By virtue of a 3D (Three-dimensional) printing technology, the biological medical high polymer material system and the in-situ generated hydrogel material system inside the stent form the interpenetrating network structure. As a novel integral joint osteochondral three-dimensional stent, the osteochondral three-dimensional stent disclosed by the invention is combined with mechanical performance and functional requirements of the stent, can effectively promote repair of each area of the cartilage and can finally achieve an integral repair purpose of the cartilage.
Description
Technical field
The present invention relates to bone cartilage three-dimensional rack and preparation method thereof, particularly a kind of bone cartilage three-dimensional rack with regular inierpeneirating network structure and preparation method thereof.
Background technology
The nineties in 20th century, American scholar Vacant and Langer propose the regenerative medicine concept of organizational project, have formed gradually now the new subject of this of tissue engineering.Tissue engineering is to utilize principle and the method for life sciences and engineering, and research and development has the clinical practice substitute of new generation of gentrify tissue or organ dysfunction, for substituting the part or all of function of tissue or organ.The development of organizational project is also for cartilage injury's reparation provides an other approach, especially solving in the problem in transplanted tissue source, in conjunction with cytokine and tissue engineering bracket, can reduce the too much dependence to transplanted tissue, cell, simplify operation technique technology, improve repairing effect.
Articular cartilage can be divided into shallow top layer, transition zone, radiating layer and calcified cartilage layer.Articular cartilage hierarchal arrangement has reflected that the biomechanics adapting with articular cartilage function changes.Shallow top layer is mainly take shearing as main, and transition region and radiation area mainly bear pressure loading, and calcified cartilage layer is attached to articular surface on bone.The cartilaginous tissue that shallow top layer comprises 10-20%, is made up of fusiformis chondrocyte and fine collagen.。This layer is divided into again cellular layer and cell free zone, and cell free zone is bright flaggy.Be positioned at top layer, acellular, form the joint face of articular cartilage, contain proteoglycan filamentous; Cellular layer is made up of flat cell and the fine collagen that holds cell.Transition zone: or cry intermediate layer, the cartilaginous tissue that has comprised 40-60%, this layer of chondrocyte is spherical in shape, and collagen fiber are diagonal and arrange.Width is penetrated layer: its cell is large, circle perpendicular to surface, is columnar arrangement, and its collagen fiber are radial arrangement between cell.Calcification layer: be close to subcartilaginous osseous lamella, this confluent monolayer cells has all features of dead cell, has crossover phenomenon between calcification layer and subcartilaginous osseous lamella surface, this layer is separated out subchondral bone and the avascular articular cartilage of blood vessel the most deeply.
Adopt tissue engineering method to build the osteochondro tissue time not long, as desirable osteochondral tissue engineering, should comprise a three-dimensional porous rack with certain mechanical property, there is in the combination of the top of support the support that promotes chondrocyte growth simultaneously, adopt the mode of this bone cartilage frame to carry out repairing in osseocartilaginous organizational project or body.
Floor district cartilage tissue engineering rack need meet the following conditions: 1) the mechanical property difference of the each floor of support; 2) aperture of each layer of support is different with porosity; 3) the degradation property difference of each layer of support; 4) biocompatibility issues of each layer of support.
Quantity research shows greatly, and the effect that is designed for the integrated reparation of bone cartilage according to the layer plot structure of cartilage is better than the simple reparation to cartilage.By relatively finding, mechanical strength and the functional of support that the existing design for bone cartilage integration organization engineering rack mainly concentrates on support are not taken into account, so the integrated recovery support of designing has many problems to await solving.
Summary of the invention
In order to overcome the above-mentioned shortcoming and deficiency of prior art, the object of the present invention is to provide a kind of bone cartilage three-dimensional rack with regular inierpeneirating network structure, in mechanical strength and internal structure, all meet the requirement of repair of cartilage, and realized the required functionalization requirement in support Ge Ceng district.
Another object of the present invention is to provide the preparation method of above-mentioned bone cartilage three-dimensional rack.
Object of the present invention is achieved through the following technical solutions:
There is a bone cartilage three-dimensional rack for regular inierpeneirating network structure, generated the inierpeneirating network structure of hydrogel material System forming rule by biological medical polymer material system and original position;
Described biological medical polymer material system is made up of biological medical polymer material and inorganic material; Described biological medical polymer material is at least one in polylactic acid, Poly(D,L-lactide-co-glycolide, polycaprolactone, polyurethane and poly butyric ester; Described inorganic material is at least one in hydroxyapatite, tricalcium phosphate, bio-vitric, calcium silicates, silicon dioxide, Graphene;
Described original position generates hydrogel material system and is made up of original position hydrogel material and drug-supplying system.
Described original position hydrogel material is at least one in temperature-responsive hydrogel, photoresponse hydrogel, pH response water gel, pressure-responsive hydrogel, electromagnetic response hydrogel.
Described drug-supplying system is at least one in bioactive molecule or medicine carrying microballoons or drug-loaded liposome or medicine carrying microcapsule.
Described bone cartilage three-dimensional rack comprises calcification layer, emitting layer, transition zone and shallow top layer, between each layer, is interconnected;
In described calcification layer, the mass percent of biological medical polymer material system is 95%-85%, and the mass percent that original position generates hydrogel material system is 5%-15%; In the biological medical polymer material system of described calcification layer, the mass percent of inorganic matter is 30%-95%, and the mass percent of biological medical polymer material is 70%-5%;
In described emitting layer, the mass percent of biological medical polymer material system is 90%-70%, and the mass percent that original position generates hydrogel material system is 10%-30%; In the biological medical polymer material system of described emitting layer, the mass percent of inorganic matter is 5%-50%, and the mass percent of biological medical polymer material is 95%-50%;
In described transition zone, the mass percent of biological medical polymer material system is 85%-40%, and the mass percent that original position generates hydrogel material system is 15%-60%; In the biological medical polymer material system of described transition zone, the mass percent of inorganic matter is 0%-10%, and the mass percent of biological medical polymer material is 100%-90%;
In described shallow top layer, the mass percent of biological medical polymer material system is 70%-20%, and the mass percent that original position generates hydrogel material system is 30%-80%; In the biological medical polymer material system on described shallow top layer, the mass percent of inorganic matter is 0%-5%, and the mass percent of biological medical polymer material is 100%-95%.
The thickness of described calcification layer is 100-1500 μ m, and emitting layer thickness is 1000-3000 μ m, and transition region thickness is 500-2000 μ m, and shallow skin depth is 100-3000 μ m.
The porosity of described calcification layer is 30%-60%, and the porosity of emitting layer is 40%-70%, and the porosity of transition zone is 50%-85%, and the porosity on shallow top layer is 60%-95%.
The aperture of described calcification layer is 50-500 μ m, and emitting layer aperture is 50-500 μ m, and the aperture of transition zone is 20-800 μ m, and aperture, shallow top layer is 20-800 μ m.
The preparation method of above-mentioned bone cartilage three-dimensional rack, realizes the preparation of the bone cartilage three-dimensional rack with regular inierpeneirating network structure by 3D printing technique.
The process by the preparation of 3D printing technique with the bone cartilage three-dimensional rack of regular inierpeneirating network structure is specially:
Select biological medical polymer material and internal stent aperture porosity according to the mechanical strength on calcification layer, emitting layer, transition zone and shallow top layer and Growth of Cells environmental requirement, the biological medical polymer material of each layer of choosing is placed in to different hoppers, the parameter of each barrel is set; According to the district's functional requirement of cartilage different layers, the original position generation hydrogel material of choosing each layer is placed in different hoppers, and the parameter of each hopper is set.Design inierpeneirating network structure, design different materials system operation order, arranges after instrument parameter working procedure.
Compared with prior art, the present invention has the following advantages and beneficial effect:
(1) the bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present invention, be a kind of novel bone cartilage integration organization engineering rack, in this support, biological medical polymer material system mainly makes that support is necessary meets bone cartilage mechanical performance and Growth of Cells environmental requirement is met; And water gel ties up to the carrier as bioactive molecule or other drug-supplying systems in support, realize the functionalization requirement in Liao Geceng district.
(2) the present invention, by the gradient design to three-dimensional rack Ge Ceng district, meets the mechanical performance in repair of cartilage Zhong Geceng district and the requirement of internal structure, and meets the Functional Requirement in repair of cartilage Zhong Geceng district by the functional supports in design Ge Ceng district.This support has been taken into account the functional requirement in mechanical performance, Growth of Cells environment He Geceng district and these character can realize larger modification scope by changing in the selection of design, bioactive molecule of material system, procedure parameter, functional supports.
Accompanying drawing explanation
Fig. 1 is the bone cartilage three-dimensional rack overall structure schematic diagram of embodiments of the invention 1.
Fig. 2 is the profile of the bone cartilage three-dimensional rack that obtains of embodiments of the invention 1.
Fig. 3 is the electronic scanner microscope picture of the transition zone of the bone cartilage three-dimensional rack of embodiments of the invention 2.
Fig. 4 is the displacement-load diagram of the bone cartilage three-dimensional rack in embodiments of the invention 2.
The specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited to this.
The bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment: the inierpeneirating network structure that is generated hydrogel material System forming rule by biological medical polymer material system and original position, comprise calcification layer, emitting layer, transition zone and shallow top layer, between each layer, be interconnected, inner void position is square.The thickness of calcification layer is 100 μ m, and the thickness of emitting layer is 1000 μ m, and the thickness of transition zone is 500 μ m, and the thickness on shallow top layer is 100 μ m.The aperture of calcification layer and emitting layer is 50 μ m, and transition zone and shallow top layer and aperture are 20 μ m.
Wherein, biological medical polymer material system is made up of polyurethane and hydroxyapatite, and in the biological medical polymer material system of calcification layer, polyurethane content is 70wt%, and the content of hydroxyapatite is 30wt%; In emitting layer biological medical polymer material system, the content of polyurethane is 95wt%, and the content of hydroxyapatite is 5wt%; In transition zone biological medical polymer material system, polyurethane content is 100wt%, and the content of hydroxyapatite is 0wt%; In the biological medical polymer material system of shallow top layer, polyurethane content is 100wt%, and the content of hydroxyapatite is 0wt%.
The temperature-sensitive hydrogel that original position generation hydrogel material system is formed by the PECE that is mixed with TGF-β 3 albumen forms.
In described calcification layer, the mass percent of biological medical polymer material system is 90%, and the mass percent that original position generates hydrogel material system is 10%; In emitting layer, the mass percent of biological medical polymer material system is 80%, and the mass percent that original position generates hydrogel material system is 20%; In transition zone, the mass percent of biological medical polymer material system is 50%, and the mass percent that original position generates hydrogel material system is 50%; In shallow top layer, the mass percent of biological medical polymer material system is 50%, and the mass percent that original position generates hydrogel material system is 50%.
The preparation method of the bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment is as follows:
(1) setting of 3D printer parameter: the thickness of setting calcification layer is 100 μ m, and the thickness of emitting layer is 1000 μ m, and the thickness of transition zone is 500 μ m, and the thickness on shallow top layer is 100 μ m.The pore space structure of setting internal stent is square; The aperture of setting calcification layer and emitting layer is 50 μ m, and setting transition zone and shallow top layer and aperture is 20 μ m, sets after complete above-mentioned parameter, and the porosity that support is each layer is roughly determined.
(2) material of preparation is put into respectively to different hoppers, set after suitable temperature and pressure, after working procedure, can obtain having the integrated bone repair of cartilage three-dimensional rack of regular inierpeneirating network structure.
Fig. 1 is the overall structure schematic diagram of the bone cartilage three-dimensional rack that obtains of the present embodiment, comprises calcification layer 1, emitting layer 2, transition zone 3 and shallow top layer 4.Fig. 2 is the profile of the bone cartilage three-dimensional rack that obtains of the present embodiment.In figure, solid line represents biological medical polymer material system, and dotted line represents that original position generates hydrogel material system.
The bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment: the inierpeneirating network structure that is generated hydrogel material System forming rule by biological medical polymer material system and original position, comprise calcification layer, emitting layer, transition zone and shallow top layer, between each layer, be interconnected, comprise calcification layer, emitting layer, transition zone and shallow top layer, between each layer, be interconnected, inner void position is square.The thickness of calcification layer is 1500 μ m, and the thickness of emitting layer is 3000 μ m, and the thickness of transition zone is 2000 μ m, and the thickness on shallow top layer is 3000 μ m.The aperture of calcification layer and emitting layer is 500 μ m, and setting transition zone and shallow top layer and aperture is 800 μ m.
Wherein, biological medical polymer material system is made up of polylactic acid and bio-vitric; In the biological medical polymer material system of calcification layer, polylactic acid content is 5wt%, and the content of bio-vitric is 95wt%; In emitting layer biological medical polymer material system, the content of polylactic acid is 50wt%, and the content of bio-vitric is 50wt%; In transition zone biological medical polymer material system, polylactic acid content is 90wt%, and the content of bio-vitric is 10wt%; In the biological medical polymer material system of shallow top layer, polylactic acid content is 5wt%, and the content of bio-vitric is 95wt%.
The original position generation hydrogel material that original position generates in hydrogel material system is PDMAEMA (polymethylacrylic acid dimethylaminoethyl)-g-PNIPAM) (poly-N-isopropyl acrylamide), there is the dual correspondence of temperature and PH.In the hydrogel material of calcification layer and emitting layer, be mixed with BMP-2 albumen, promote calcification and skeletonization that this is two-layer; In the hydrogel material on transition zone and shallow top layer, be mixed with TGF-β 1 albumen, promote this two-layer one-tenth chondrify.
In described calcification layer, the mass percent of biological medical polymer material system is 95%, and the mass percent that original position generates hydrogel material system is 5%; In emitting layer, the mass percent of biological medical polymer material system is 85%, and the mass percent that original position generates hydrogel material system is 15%; In transition zone, the mass percent of biological medical polymer material system is 75%, and the mass percent that original position generates hydrogel material system is 25%; In shallow top layer, the mass percent of biological medical polymer material system is 65%, and the mass percent that original position generates hydrogel material system is 35%.
The preparation method of the bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment is as follows:
(1) setting of 3D printer parameter: the thickness of setting calcification layer is 1500 μ m, and the thickness of emitting layer is 3000 μ m, and the thickness of transition zone is 2000 μ m, and the thickness on shallow top layer is 3000 μ m; The pore space structure of setting internal stent is square; The aperture of setting calcification layer and emitting layer is 500 μ m, and setting transition zone and shallow top layer and aperture is 800 μ m.Set after complete above-mentioned parameter, the porosity that support is each layer is roughly determined.
(2) material of preparation is put into respectively to different hoppers, set after suitable temperature and pressure, after working procedure, can obtain having the integrated bone repair of cartilage three-dimensional rack of regular inierpeneirating network structure.
Fig. 3 is the electronic scanner microscope picture of the transition zone of the bone cartilage three-dimensional rack of the present embodiment.In designed bone cartilage three-dimensional rack, pars fibrosa outthrust is bio-vitric as seen from the figure, and the bonding that the connected sum of support mesopore is each layer is all right, is easy to adhesion and the propagation of cell.
Fig. 4 is the displacement-load diagram of the bone cartilage three-dimensional rack of the present embodiment.The support of the present embodiment is of a size of 10*10*6mm, in the time being compressed load, there is elastic deformation in support, as can be seen from the figure at deformation quantity hour, the elastic deformation of support meets Hooke's law to the support of design, and this support is surrendered when compared with large deformation amount.Be about 7MPa by the modulus of compressibility that calculates support entirety, meet the requirement of bone repair of cartilage.
The bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment: the inierpeneirating network structure that is generated hydrogel material System forming rule by biological medical polymer material system and original position, comprise calcification layer, emitting layer, transition zone and shallow top layer, between each layer, be interconnected, inner void position is square.The thickness of calcification layer is 500 μ m, and the thickness of emitting layer is 2000 μ m, and the thickness of transition zone is 1500 μ m, and the thickness on shallow top layer is 2500 μ m.The pore space structure of setting internal stent is square; The aperture of setting calcification layer and emitting layer is 450 μ m, and setting transition zone and shallow top layer and aperture is 200 μ m.
Wherein, biological medical polymer material system is made up of polycaprolactone and tricalcium phosphate; In the biological medical polymer material system of calcification layer, polycaprolactone content is 60wt%, and the content of tricalcium phosphate is 50wt%; In emitting layer biological medical polymer material system, the content of polycaprolactone is 45wt%, and the content of tricalcium phosphate is 55wt%; In transition zone biological medical polymer material system, polycaprolactone content is 95wt%, and the content of tricalcium phosphate is 5wt%; In the biological medical polymer material system of shallow top layer, polycaprolactone content is 98wt%, and the content of tricalcium phosphate is 2wt%.
In described calcification layer, the mass percent of biological medical polymer material system is 85%, and the mass percent that original position generates hydrogel material system is 15%; In emitting layer, the mass percent of biological medical polymer material system is 85%, and the mass percent that original position generates hydrogel material system is 15%; In transition zone, the mass percent of biological medical polymer material system is 80%, and the mass percent that original position generates hydrogel material system is 20%; In shallow top layer, the mass percent of biological medical polymer material system is 40%, and the mass percent that original position generates hydrogel material system is 60%.
The preparation method of the bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment is as follows:
(1) setting of 3D printer parameter: the thickness of setting calcification layer is 500 μ m, and the thickness of emitting layer is 2000 μ m, and the thickness of transition zone is 1500 μ m, and the thickness on shallow top layer is 2500 μ m.The pore space structure of setting internal stent is square; The aperture of setting calcification layer and emitting layer is 450 μ m, and setting transition zone and shallow top layer and aperture is 200 μ m.Set after complete above-mentioned parameter, the porosity that support is each layer is roughly determined.
(2) material of preparation is put into respectively to different hoppers, set after suitable temperature and pressure, after working procedure, can obtain having the integrated bone repair of cartilage three-dimensional rack of regular inierpeneirating network structure.
The bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment: the inierpeneirating network structure that is generated hydrogel material System forming rule by biological medical polymer material system and original position, comprise calcification layer, emitting layer, transition zone and shallow top layer, between each layer, be interconnected, inner void position is square.The thickness of calcification layer is 800 μ m, and the thickness of emitting layer is 1500 μ m, and the thickness of transition zone is 700 μ m, and the thickness on shallow top layer is 1500 μ m.The aperture of calcification layer and emitting layer is 350 μ m, and setting transition zone and shallow top layer and aperture is 550 μ m.
Wherein, biological medical polymer material system is made up of PLGA and calcium silicates.In the biological medical polymer material system of calcification layer, PLGA content is 65wt%, and the content of calcium silicates is 35wt%; In emitting layer biological medical polymer material system, the content of PLGA is 35wt%, and the content of calcium silicates is 65wt%; In transition zone biological medical polymer material system, PLGA content is 97wt%, and the content of calcium silicates is 3wt%; In the biological medical polymer material system of shallow top layer, PLGA content is 96wt%, and the content of calcium silicates is 4wt%.
The original position generation hydrogel material that original position generates in hydrogel material system is N-isopropylacrylic acid amide temperature-responsive response water gel, is mixed with BMP-7 albumen in the hydrogel material of calcification layer and emitting layer, promotes calcification and skeletonization that this is two-layer; In the hydrogel material on transition zone and shallow top layer, be mixed with TGF-β 1 albumen, promote this two-layer one-tenth chondrify.
In described calcification layer, the mass percent that accounts for of biological medical polymer material system is 90%, and the mass percent that original position generates hydrogel material system is 10%; In emitting layer, the mass percent of biological medical polymer material system is 70%, and the mass percent that original position generates hydrogel material system is 30%; In transition zone, the mass percent of biological medical polymer material system is 40%, and the mass percent that original position generates hydrogel material system is 60%; In shallow top layer, the mass percent of biological medical polymer material system is 20%, and the mass percent that original position generates hydrogel material system is 80%.
The preparation method of the bone cartilage three-dimensional rack with regular inierpeneirating network structure of the present embodiment is as follows:
(1) setting of 3D printer parameter: the thickness of setting calcification layer is 800 μ m, and the thickness of emitting layer is 1500 μ m, and the thickness of transition zone is 700 μ m, and the thickness on shallow top layer is 1500 μ m.The pore space structure of setting internal stent is square; The aperture of setting calcification layer and emitting layer is 350 μ m, and setting transition zone and shallow top layer and aperture is 550 μ m.Set after complete above-mentioned parameter, the porosity that support is each layer is roughly determined.
(2) material of preparation is put into respectively to different hoppers, set after suitable temperature and pressure, after working procedure, can obtain having the integrated bone repair of cartilage three-dimensional rack of regular inierpeneirating network structure.
The original position generation hydrogel material that original position generates in hydrogel material system is polyacrylamide pH response water gel, is mixed with BMP-2 albumen in the hydrogel material of calcification layer and emitting layer, promotes calcification and skeletonization that this is two-layer; In the hydrogel material on transition zone and shallow top layer, be mixed with IGF-1 albumen, promote this two-layer one-tenth chondrify.
In above-described embodiment, at least one in biological medical polymer material temperature-responsive hydrogel, photoresponse hydrogel, pH response water gel, pressure-responsive hydrogel, electromagnetic response hydrogel; Drug-supplying system be bioactive molecule or medicine carrying microballoons or drug-loaded liposome or medicine carrying microcapsule at least one.
Above-described embodiment is preferably embodiment of the present invention; but embodiments of the present invention are not limited by the examples; other any do not deviate from change, the modification done under spirit of the present invention and principle, substitutes, combination, simplify; all should be equivalent substitute mode, within being included in protection scope of the present invention.
Claims (8)
1. a bone cartilage three-dimensional rack with regular inierpeneirating network structure, is characterized in that, is generated the inierpeneirating network structure of hydrogel material System forming rule by biological medical polymer material system and original position;
Described biological medical polymer material system is made up of biological medical polymer material and inorganic material; Described biological medical polymer material is at least one in polylactic acid, Poly(D,L-lactide-co-glycolide, polycaprolactone, polyurethane and poly butyric ester; Described inorganic material is at least one in hydroxyapatite, tricalcium phosphate, bio-vitric, calcium silicates, silicon dioxide, Graphene;
Described original position generates hydrogel material system and is made up of original position hydrogel material and drug-supplying system.
2. the bone cartilage three-dimensional rack with regular inierpeneirating network structure according to claim 1, it is characterized in that, described original position hydrogel material is at least one in temperature-responsive hydrogel, photoresponse hydrogel, pH response water gel, pressure-responsive hydrogel, electromagnetic response hydrogel.
3. the bone cartilage three-dimensional rack with regular inierpeneirating network structure according to claim 1, is characterized in that, described drug-supplying system is at least one in bioactive molecule or medicine carrying microballoons or drug-loaded liposome or medicine carrying microcapsule.
4. the bone cartilage three-dimensional rack with regular inierpeneirating network structure according to claim 1, is characterized in that, described bone cartilage three-dimensional rack comprises calcification layer, emitting layer, transition zone and shallow top layer, between each layer, is interconnected;
In described calcification layer, the mass percent of biological medical polymer material system is 95%-85%, and the mass percent that original position generates hydrogel material system is 5%-15%; In the biological medical polymer material system of described calcification layer, the mass percent of inorganic matter is 30%-95%, and the mass percent of biological medical polymer material is 70%-5%;
In described emitting layer, the mass percent of biological medical polymer material system is 90%-70%, and the mass percent that original position generates hydrogel material system is 10%-30%; In the biological medical polymer material system of described emitting layer, the mass percent of inorganic matter is 5%-50%, and the mass percent of biological medical polymer material is 95%-50%;
In described transition zone, the mass percent of biological medical polymer material system is 85%-40%, and the mass percent that original position generates hydrogel material system is 15%-60%; In the biological medical polymer material system of described transition zone, the mass percent of inorganic matter is 0%-10%, and the mass percent of biological medical polymer material is 100%-90%;
In described shallow top layer, the mass percent of biological medical polymer material system is 70%-20%, and the mass percent that original position generates hydrogel material system is 30%-80%; In the biological medical polymer material system on described shallow top layer, the mass percent of inorganic matter is 0%-5%, and the mass percent of biological medical polymer material is 100%-95%.
5. the bone cartilage three-dimensional rack with regular inierpeneirating network structure according to claim 1, it is characterized in that, the thickness of described calcification layer is 100-1500 μ m, and emitting layer thickness is 1000-3000 μ m, transition region thickness is 500-2000 μ m, and shallow skin depth is 100-3000 μ m.
6. the bone cartilage three-dimensional rack with regular inierpeneirating network structure according to claim 1, it is characterized in that, the porosity of described calcification layer is 30%-60%, and the porosity of emitting layer is 40%-70%, the porosity of transition zone is 50%-85%, and the porosity on shallow top layer is 60%-95%.
7. the bone cartilage three-dimensional rack with regular inierpeneirating network structure according to claim 1, it is characterized in that, the aperture of described calcification layer is 50-500 μ m, and emitting layer aperture is 50-500 μ m, the aperture of transition zone is 20-800 μ m, and aperture, shallow top layer is 20-800 μ m.
8. the preparation method described in claim 1～7 any one with the bone cartilage three-dimensional rack of regular inierpeneirating network structure, is characterized in that, realizes the preparation of the bone cartilage three-dimensional rack with regular inierpeneirating network structure by 3D printing technique.
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| CN111450324A (en) * | 2020-05-26 | 2020-07-28 | 南京鼓楼医院 | Bone fusion hydrogel scaffold and preparation method thereof |
| CN115887775A (en) * | 2022-11-24 | 2023-04-04 | 吉林大学 | Gradient 3D printing polycaprolactone modified scaffold for growth plate repair and preparation method and application thereof |
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