CN103881921A - Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin - Google Patents

Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin Download PDF

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Publication number
CN103881921A
CN103881921A CN201210561604.4A CN201210561604A CN103881921A CN 103881921 A CN103881921 A CN 103881921A CN 201210561604 A CN201210561604 A CN 201210561604A CN 103881921 A CN103881921 A CN 103881921A
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China
Prior art keywords
parts
astaxanthin
bbm
improved
formula
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Pending
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CN201210561604.4A
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Chinese (zh)
Inventor
张述智
夏伟
朱绍辉
张�浩
王晓丽
徐权汗
李之详
许团辉
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Qingdao Zhongren Pharmaceutical Coltd
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Qingdao Zhongren Pharmaceutical Coltd
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Priority to CN201210561604.4A priority Critical patent/CN103881921A/en
Publication of CN103881921A publication Critical patent/CN103881921A/en
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Abstract

The invention discloses an improved BBM medium formula for Haematococcus pluvialis which is capable of high yielding of astaxanthin. The formula comprises, by mass, 0.9 to 1.1 parts of NaNO3, 0.9 to 1.1 parts of K2HPO4, 0.9 to 1.1 parts of MgSO4.7H2O, 0.9 to 1.1 parts of CaCl2.2H2O, 0.9 to 1.1 parts of KH2PO4, 0.9 to 1.1 parts of NaCl, 0.9 to 1.1 parts of B1, 0.225 to 0.275 part of vitamin H, 0.225 to 0.275 part of B12 and 0.9 to 1.1 parts of a trace element. According to the invention, NaHCO3, vitamin B12 and Fe3+ are added on the basis of a conventional BBM formula, the growth speed of cultured Haematococcus pluvialis is greatly increased, and astaxanthin yield is greatly improved.

Description

The Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin
 
Technical field
The invention belongs to micro-algae productive culture base technical field, be specifically related to the Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin.
 
Background technology
Haematocoocus Pluvialls is a kind of unicell green alga, and accumulation astaxanthin in a large number is under given conditions the highest biology (10-30mg/g) of occurring in nature accumulation natural astaxanthin ability.Natural astaxanthin has superpower anti-oxidant activity, there is the good reputation of " supper vitamin E ", can be used as fodder additives, natural food colour and biological health-care product, medicine etc., throughput far can not be met the need of market at present, therefore utilizes Haematococcus pluvialis production natural astaxanthin to receive much attention at home and abroad.It is slow that the culture medium prescription ubiquity of existing cultivation Haematocoocus Pluvialls is cultivated the speed of growth of Haematocoocus Pluvialls, the defect that astaxanthin productive rate is low.
 
Summary of the invention
The above-mentioned defect existing in order to overcome prior art field, the object of the invention is to, a kind of Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin is provided, it is slow that the culture medium prescription ubiquity of solution prior art cultivation Haematocoocus Pluvialls is cultivated the speed of growth of Haematocoocus Pluvialls, the problem that astaxanthin productive rate is low.
The Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin provided by the invention, its components in mass portion proportioning is: NaNO 30.9-1.1 parts, K 2hPO 40.9-1.1 parts, MgSO 47H 20.9-1.1 parts of O, CaCl 22H 20.9-1.1 parts of O, KH 2pO 40.9-1.1 parts, 0.9-1.1 parts of NaCl, 0.9-1.1 parts of B1,0.225-0.275 part of vitamin H, 0.225-0.275 part of B12,0.9-1.1 parts of trace elements; Described trace element comprises Na 2eDTA, MnCl 24H 2o, ZnCl 27H 2o, Na 2moO 42H 2o, FeCl 36 H 2o, CoCl 26H 2o.
The Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin provided by the invention, its beneficial effect is, on original BBM formula basis, adds NaHCO again 3, vitamin B12 and Fe3+, the speed of growth of cultivating Haematocoocus Pluvialls improves greatly, astaxanthin productive rate also improves greatly.
 
Embodiment
Below in conjunction with an embodiment, the Haematocoocus Pluvialls BBM improved culture medium formula of high-yield astaxanthin provided by the invention is described in detail.
 
Embodiment
The Haematocoocus Pluvialls BBM improved culture medium formula of the high-yield astaxanthin of the present embodiment, NaNO 31 part, K 2hPO 41 part, MgSO 47H 21 part of O, CaCl 22H 21 part of O, KH 2pO 41 part, 1 part of NaCl, 1 part of B1,0.25 part of vitamin H, 0.25 part of B12,1 part of trace element; Described trace element comprises Na 2eDTA, MnCl 24H 2o, ZnCl 27H 2o, Na 2moO 42H 2o, FeCl 36 H 2o, CoCl 26H 2o.Astaxanthin productive rate is 24.50mg/L.

Claims (2)

1. the Haematocoocus Pluvialls BBM improved culture medium of high-yield astaxanthin formula, is characterized in that: its components in mass portion proportioning is: NaNO 30.9-1.1 parts, K 2hPO 40.9-1.1 parts, MgSO 47H 20.9-1.1 parts of O, CaCl 22H 20.9-1.1 parts of O, KH 2pO 40.9-1.1 parts, 0.9-1.1 parts of NaCl, 0.9-1.1 parts of B1,0.225-0.275 part of vitamin H, 0.225-0.275 part of B12,0.9-1.1 parts of trace elements.
2. the Haematocoocus Pluvialls BBM improved culture medium of high-yield astaxanthin according to claim 1 formula, is characterized in that:
Described trace element comprises Na 2eDTA, MnCl 24H 2o, ZnCl 27H 2o, Na 2moO 42H 2o, FeCl 36 H 2o, CoCl 26H 2o.
CN201210561604.4A 2012-12-22 2012-12-22 Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin Pending CN103881921A (en)

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CN201210561604.4A CN103881921A (en) 2012-12-22 2012-12-22 Improved BBM medium formula for Haematococcus pluvialis capable of high yielding of astaxanthin

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104762211A (en) * 2015-05-04 2015-07-08 厦门大学 Wall-breaking method for haematococcus pluvialis
CN105420332A (en) * 2015-12-10 2016-03-23 天津科技大学 Method for achieving high yield of astaxanthin through haematococcus pluvialis
CN105567625A (en) * 2016-02-26 2016-05-11 中国水产科学研究院长江水产研究所 Haematococcus pluvialis induction medium
CN107460225A (en) * 2017-10-11 2017-12-12 广西南宁秀珀生物科技有限公司 The method of continuous culture patter of rain haematococcus accumulation astaxanthin
CN107502551A (en) * 2017-10-11 2017-12-22 广西南宁秀珀生物科技有限公司 Utilize the method for bioreactor culture patter of rain haematococcus
CN107502646A (en) * 2017-10-11 2017-12-22 广西南宁秀珀生物科技有限公司 Utilize the method for patter of rain haematococcus efficient accumulation astaxanthin
CN107523503A (en) * 2017-10-11 2017-12-29 广西南宁秀珀生物科技有限公司 The cultural method of patter of rain haematococcus

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104762211A (en) * 2015-05-04 2015-07-08 厦门大学 Wall-breaking method for haematococcus pluvialis
CN105420332A (en) * 2015-12-10 2016-03-23 天津科技大学 Method for achieving high yield of astaxanthin through haematococcus pluvialis
CN105567625A (en) * 2016-02-26 2016-05-11 中国水产科学研究院长江水产研究所 Haematococcus pluvialis induction medium
CN107460225A (en) * 2017-10-11 2017-12-12 广西南宁秀珀生物科技有限公司 The method of continuous culture patter of rain haematococcus accumulation astaxanthin
CN107502551A (en) * 2017-10-11 2017-12-22 广西南宁秀珀生物科技有限公司 Utilize the method for bioreactor culture patter of rain haematococcus
CN107502646A (en) * 2017-10-11 2017-12-22 广西南宁秀珀生物科技有限公司 Utilize the method for patter of rain haematococcus efficient accumulation astaxanthin
CN107523503A (en) * 2017-10-11 2017-12-29 广西南宁秀珀生物科技有限公司 The cultural method of patter of rain haematococcus

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Application publication date: 20140625